Starting /dee2/code/volunteer_pipeline.sh ERR6133441 current disk space = 1545973211136 free memory = 1440154692 ERR6133441 SRAfilesize fcf6cdaf89244ebc803015220fd63878 ERR6133441.sra ERR6133441.sra file validated ERR6133441 is single end ERR6133441 is conventional basespace ERR6133441 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133441_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.98625 37.0 33.0 37.0 33.0 37.0 2 36.22575 37.0 37.0 37.0 33.0 37.0 3 35.63875 37.0 37.0 37.0 33.0 37.0 4 35.4495 37.0 37.0 37.0 33.0 37.0 5 35.3645 37.0 37.0 37.0 33.0 37.0 6 35.635 37.0 37.0 37.0 33.0 37.0 7 37.33075 37.0 37.0 40.0 33.0 40.0 8 37.341 37.0 37.0 40.0 33.0 40.0 9 37.36 37.0 37.0 40.0 33.0 40.0 10-11 37.359875 37.0 37.0 40.0 33.0 40.0 12-13 37.21275 37.0 37.0 40.0 33.0 40.0 14-15 37.224625 37.0 37.0 40.0 33.0 40.0 16-17 37.167500000000004 37.0 37.0 40.0 33.0 40.0 18-19 37.37925 37.0 37.0 40.0 33.0 40.0 20-21 37.49325 37.0 37.0 40.0 33.0 40.0 22-23 37.407875000000004 37.0 37.0 40.0 33.0 40.0 24-25 37.370374999999996 37.0 37.0 40.0 33.0 40.0 26-27 37.176875 37.0 37.0 40.0 33.0 40.0 28-29 37.23775 37.0 37.0 40.0 33.0 40.0 30-31 37.225625 37.0 37.0 40.0 33.0 40.0 32-33 37.03425 37.0 37.0 40.0 33.0 40.0 34-35 36.87525 37.0 37.0 40.0 33.0 40.0 36-37 36.783874999999995 37.0 37.0 40.0 33.0 40.0 38-39 36.74925 37.0 37.0 40.0 33.0 40.0 40-41 36.632125 37.0 37.0 40.0 33.0 40.0 42-43 36.4815 37.0 37.0 40.0 33.0 40.0 44-45 36.21225 37.0 37.0 40.0 33.0 40.0 46-47 36.199749999999995 37.0 37.0 38.5 33.0 40.0 48-49 35.9985 37.0 35.0 37.0 33.0 40.0 50-51 35.826625 37.0 33.0 37.0 33.0 40.0 52-53 35.732875 37.0 33.0 37.0 33.0 40.0 54-55 35.518375 37.0 33.0 37.0 33.0 40.0 56-57 35.454750000000004 37.0 33.0 37.0 33.0 38.5 58-59 35.062625 37.0 33.0 37.0 33.0 37.0 60-61 34.96725 37.0 33.0 37.0 33.0 37.0 62-63 34.862625 37.0 33.0 37.0 33.0 37.0 64-65 34.38675 37.0 33.0 37.0 30.0 37.0 66-67 34.329375 37.0 33.0 37.0 27.0 37.0 68-69 33.054375 35.0 33.0 35.0 27.0 37.0 70-71 33.39952710315473 33.0 33.0 37.0 27.0 37.0 72-73 34.29251982466737 37.0 33.0 37.0 33.0 37.0 74-75 34.46892994420884 37.0 33.0 37.0 33.0 37.0 76-77 34.36029369624181 37.0 33.0 37.0 33.0 37.0 78-79 34.31978665124495 37.0 33.0 37.0 33.0 37.0 80-81 34.21697209692674 37.0 33.0 37.0 33.0 37.0 82-83 33.93628039146425 37.0 33.0 37.0 27.0 37.0 84-85 33.992977836616795 37.0 33.0 37.0 30.0 37.0 86-87 34.07204794695231 37.0 33.0 37.0 33.0 37.0 88-89 33.94108645753634 37.0 33.0 37.0 30.0 37.0 90-91 33.77926549349656 35.0 33.0 37.0 27.0 37.0 92-93 33.5828870186177 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 10.0 22 8.0 23 13.0 24 20.0 25 23.0 26 30.0 27 28.0 28 46.0 29 60.0 30 71.0 31 99.0 32 140.0 33 181.0 34 227.0 35 489.0 36 962.0 37 1141.0 38 446.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 87.35000000000001 2.4 2.4250000000000003 7.825 2 69.70455683525289 18.97846770155233 6.509764646970456 4.8072108162243365 3 35.6 39.275 14.174999999999999 10.95 4 35.875 27.1 17.775 19.25 5 25.974999999999998 31.0 23.849999999999998 19.175 6 21.325 37.2 24.325 17.150000000000002 7 36.05 29.7 19.15 15.1 8 30.45 30.55 23.0 16.0 9 25.825 28.075 27.05 19.05 10-11 26.325 27.750000000000004 26.5125 19.412499999999998 12-13 28.95 25.6125 26.724999999999998 18.712500000000002 14-15 22.537499999999998 28.625 29.475 19.3625 16-17 24.6625 31.2125 25.775 18.35 18-19 24.4 26.337500000000002 26.887499999999996 22.375 20-21 25.387500000000003 26.35 27.037499999999998 21.224999999999998 22-23 27.037499999999998 24.175 27.250000000000004 21.5375 24-25 27.474999999999998 24.1625 27.3 21.0625 26-27 25.937500000000004 24.462500000000002 29.599999999999998 20.0 28-29 26.75 26.0 26.337500000000002 20.9125 30-31 26.087500000000002 26.087500000000002 26.487500000000004 21.337500000000002 32-33 23.9875 27.950000000000003 27.075 20.9875 34-35 26.224999999999998 24.6625 27.487499999999997 21.625 36-37 25.0375 24.0625 29.475 21.425 38-39 25.687500000000004 25.4625 29.125 19.725 40-41 26.8 25.387500000000003 26.387500000000003 21.425 42-43 24.925 28.275 26.8 20.0 44-45 23.35 25.674999999999997 28.749999999999996 22.225 46-47 24.762500000000003 23.8125 28.375 23.05 48-49 25.7125 23.95 30.0875 20.25 50-51 25.974999999999998 26.174999999999997 28.249999999999996 19.6 52-53 25.7875 25.687500000000004 27.962500000000002 20.5625 54-55 24.087500000000002 28.325 28.125 19.4625 56-57 25.3125 24.712500000000002 28.9 21.075 58-59 24.087500000000002 24.125 29.75 22.037499999999998 60-61 25.025 24.099999999999998 29.7375 21.1375 62-63 23.0375 27.250000000000004 30.7375 18.975 64-65 24.0125 26.0 29.799999999999997 20.1875 66-67 25.112499999999997 25.4875 29.562500000000004 19.8375 68-69 23.75 26.950000000000003 27.787499999999998 21.512500000000003 70-71 25.69427070302727 25.01876407305479 28.471353515136354 20.815611708781585 72-73 26.42409033877039 24.680050188205772 27.854454203262236 21.041405269761608 74-75 23.840060354583176 26.103357223689173 28.957626053061738 21.09895636866591 76-77 22.82471626733922 26.82219419924338 30.56746532156368 19.78562421185372 78-79 24.3451853726433 24.851322282677465 30.266987220043024 20.536505124636214 80-81 23.81435455237129 26.946487446107025 29.710880040578243 19.528277960943445 82-83 23.60793287566743 24.75209763539283 29.82456140350877 21.815408085430967 84-85 24.059191223370327 24.569460390355914 30.271718331419823 21.099630054853936 86-87 23.603672532517216 25.847997959704156 31.446059678653405 19.102269829125223 88-89 22.736546799285897 27.582249426166793 30.209130323896964 19.472073450650345 90-91 25.172149961744456 25.197653659780666 29.890334098444278 19.739862280030604 92-93 24.509053812802854 27.824534557510837 28.71716398877837 18.94924764090793 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 5.0 18 7.0 19 2.0 20 0.5 21 2.0 22 2.5 23 4.5 24 6.5 25 3.5 26 5.5 27 10.5 28 13.0 29 14.5 30 20.5 31 25.5 32 36.5 33 58.0 34 71.5 35 79.5 36 98.0 37 132.5 38 154.5 39 157.5 40 177.5 41 186.0 42 199.0 43 222.0 44 205.0 45 196.0 46 223.5 47 210.0 48 176.5 49 175.5 50 168.0 51 163.5 52 147.5 53 139.5 54 125.0 55 93.0 56 80.0 57 75.0 58 73.0 59 58.5 60 44.0 61 45.5 62 42.5 63 32.0 64 31.0 65 35.0 66 31.0 67 22.5 68 17.0 69 13.0 70 6.5 71 2.0 72 1.0 73 0.0 74 2.0 75 3.0 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.15 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.06374298827129016 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 6.0 71 6.0 72 6.0 73 2.0 74 7.0 75 4.0 76 8.0 77 6.0 78 7.0 79 4.0 80 2.0 81 8.0 82 2.0 83 9.0 84 2.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3921.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.075 #Duplication Level Percentage of deduplicated Percentage of total 1 94.42346790589602 81.27499999999999 2 3.5724658727853615 6.15 3 0.7261109497531223 1.875 4 0.4066221318617485 1.4000000000000001 5 0.17426662794074935 0.75 6 0.17426662794074935 0.8999999999999999 7 0.02904443799012489 0.17500000000000002 8 0.02904443799012489 0.2 9 0.0 0.0 >10 0.46471100784199826 7.2749999999999995 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 40 1.0 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 39 0.975 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 22 0.5499999999999999 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 21 0.525 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 21 0.525 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 20 0.5 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 17 0.42500000000000004 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 15 0.375 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 15 0.375 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 15 0.375 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 13 0.325 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 11 0.27499999999999997 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 11 0.27499999999999997 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 11 0.27499999999999997 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 10 0.25 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 10 0.25 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 8 0.2 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 7 0.17500000000000002 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 6 0.15 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 6 0.15 No Hit GGAGTCCCATATATATATGTATAAGATGCCAGCCCGGTTTCGTCAACCAT 6 0.15 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 6 0.15 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 6 0.15 No Hit GGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTG 6 0.15 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 5 0.125 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 5 0.125 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 5 0.125 No Hit GGGGAAGAAGACCTCTTTCTGGGAGGCCGAAGCCACTTCGGCACCGGCAC 5 0.125 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 5 0.125 No Hit GGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0125 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.0625 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245086 READS because READLEN < 1 Read 245086 spots for ERR6133441.sra Written 245086 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra Rejected 245074 READS because READLEN < 1 Read 245074 spots for ERR6133441.sra Written 245074 spots for ERR6133441.sra SRR ids: ['ERR6133441.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cz7d2dp6 ERR6133441.sra spots: 4901492 blocks: [[1, 245074], [245075, 490148], [490149, 735222], [735223, 980296], [980297, 1225370], [1225371, 1470444], [1470445, 1715518], [1715519, 1960592], [1960593, 2205666], [2205667, 2450740], [2450741, 2695814], [2695815, 2940888], [2940889, 3185962], [3185963, 3431036], [3431037, 3676110], [3676111, 3921184], [3921185, 4166258], [4166259, 4411332], [4411333, 4656406], [4656407, 4901492]] ERR6133441 file size 1086209 ERR6133441 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133441 ERR6133441_1.fastq Input file: ERR6133441_1.fastq trimmed: ERR6133441-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 05:44:50 2024 >> started Sat Dec 7 05:44:53 2024 >> done (2.514s) 4901492 reads processed; of these: 1823 ( 0.04%) short reads filtered out after trimming by size control 23 ( 0.00%) empty reads filtered out after trimming by size control 4899646 (99.96%) reads available; of these: 65840 ( 1.34%) trimmed reads available after processing 4833806 (98.66%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 34 0.00% 19 59 0.00% 20 33 0.00% 21 16 0.00% 22 20 0.00% 23 14 0.00% 24 9 0.00% 25 15 0.00% 26 4 0.00% 27 15 0.00% 28 6 0.00% 29 141 0.00% 30 12 0.00% 31 7 0.00% 32 11 0.00% 33 9 0.00% 34 6 0.00% 35 5 0.00% 36 8 0.00% 37 13 0.00% 38 14 0.00% 39 24 0.00% 40 73 0.00% 41 37 0.00% 42 20 0.00% 43 73 0.00% 44 34 0.00% 45 20 0.00% 46 28 0.00% 47 26 0.00% 48 28 0.00% 49 24 0.00% 50 26 0.00% 51 37 0.00% 52 31 0.00% 53 23 0.00% 54 32 0.00% 55 22 0.00% 56 18 0.00% 57 22 0.00% 58 14 0.00% 59 26 0.00% 60 22 0.00% 61 27 0.00% 62 4 0.00% 63 5 0.00% 64 4 0.00% 65 4 0.00% 66 7 0.00% 67 11 0.00% 68 31 0.00% 69 93 0.00% 70 6243 0.13% 71 6039 0.12% 72 6370 0.13% 73 5969 0.12% 74 6192 0.13% 75 5961 0.12% 76 5523 0.11% 77 5701 0.12% 78 6298 0.13% 79 6886 0.14% 80 6279 0.13% 81 6393 0.13% 82 7035 0.14% 83 7982 0.16% 84 6217 0.13% 85 133 0.00% 86 251 0.01% 87 426 0.01% 88 795 0.02% 89 1555 0.03% 90 3682 0.08% 91 10771 0.22% 92 45545 0.93% 93 4740103 96.74% 4899646 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=6.87 fanout-score-rank=21 prefix-density=0.69 prefix-fanout=2.8 sequence=GCCGCCGCCGCCAAGGAAGGCATGTTCGTCAAGAACTACAGCTACTGATCCTAATCGCATCAAGCTTCAACGCCTGTGAGTGAAAACCAGTGATGAGAGTGCTGCTGCTAGCTAGCGCCGGCATTGATGAGCTTGAGAGGGCACTGTAGCCAGTGTGTCAGTCGTTGTTAAATTACAGGTTGAGATCATCAGCGTACTCCGATGGGAGGTGGACATCAGAAAGTATACTGTGTTTTACCACCCTAATTAAGTAAACAACTTTTGGAATGGTGATCATCTTACTGTTTTAAATAAATCTGTTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=74.92 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=1.2 sequence=CCGCCAAGGCGCTGGGCTCGTTCAGGAGCACCGGCGCTGCGACCGCACGAACCTAACCAAGGAGCGAGCACACACGTACGCATGCATCCGTGTAATCGAAGCTGCTTAATTTGGTCGGCCCGGGCGTCCCTGTTTGATTCTTGGTTCTACTACTTTGCTTGGTTCATGCT Started job on | Dec 07 05:45:12 Started mapping on | Dec 07 05:45:12 Finished on | Dec 07 05:45:19 Mapping speed, Million of reads per hour | 2519.82 Number of input reads | 4899646 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 3771405 Uniquely mapped reads % | 76.97% Average mapped length | 92.33 Number of splices: Total | 216203 Number of splices: Annotated (sjdb) | 179799 Number of splices: GT/AG | 208315 Number of splices: GC/AG | 4943 Number of splices: AT/AC | 81 Number of splices: Non-canonical | 2864 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.04% Deletion average length | 1.78 Insertion rate per base | 0.02% Insertion average length | 2.28 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1036720 % of reads mapped to multiple loci | 21.16% Number of reads mapped to too many loci | 18768 % of reads mapped to too many loci | 0.38% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.46% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 91521 91521 91521 N_multimapping 1036720 1036720 1036720 N_noFeature 207221 243163 3601664 N_ambiguous 148116 14428 359 UnstrandedReadsAssigned:3416068 PositiveStrandReadsAssigned:3513814 NegativeStrandReadsAssigned:169382 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133441 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133441-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 4,899,646 reads, 4,303,994 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,073 rounds 52973 ERR6133441.ke.tsv 35125 ERR6133441.se.tsv 88098 total ==> ERR6133441.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 152 34.578 PNS24243 293 194 0 0 KQK14069 1603 1504 342.964 71.1724 KQK14071 474 375 1.03591 0.862185 ==> ERR6133441.se.tsv <== BRADI_1g14170v3 343 BRADI_1g53295v3 112 BRADI_1g59795v3 40 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 55 BRADI_1g74790v3 44 BRADI_1g09890v3 0 BRADI_1g77505v3 107 BRADI_1g48960v3 2 ERR6133441 completed mapping pipeline successfully