Starting /dee2/code/volunteer_pipeline.sh ERR6133444 current disk space = 1545742716928 free memory = 1420665460 ERR6133444 SRAfilesize 113c82323a0caf8445a3c144c1a1eea7 ERR6133444.sra ERR6133444.sra file validated ERR6133444 is single end ERR6133444 is conventional basespace ERR6133444 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133444_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.1405 37.0 33.0 37.0 33.0 37.0 2 36.277 37.0 37.0 37.0 33.0 37.0 3 35.79575 37.0 37.0 37.0 33.0 37.0 4 35.53825 37.0 37.0 37.0 33.0 37.0 5 35.493 37.0 37.0 37.0 33.0 37.0 6 35.7425 37.0 37.0 37.0 33.0 37.0 7 37.47275 37.0 37.0 40.0 33.0 40.0 8 37.48625 37.0 37.0 40.0 33.0 40.0 9 37.5295 37.0 37.0 40.0 33.0 40.0 10-11 37.426874999999995 37.0 37.0 40.0 33.0 40.0 12-13 37.358999999999995 37.0 37.0 40.0 33.0 40.0 14-15 37.30525 37.0 37.0 40.0 33.0 40.0 16-17 37.237624999999994 37.0 37.0 40.0 33.0 40.0 18-19 37.405249999999995 37.0 37.0 40.0 33.0 40.0 20-21 37.559 37.0 37.0 40.0 33.0 40.0 22-23 37.416125 37.0 37.0 40.0 33.0 40.0 24-25 37.471999999999994 37.0 37.0 40.0 33.0 40.0 26-27 37.29075 37.0 37.0 40.0 33.0 40.0 28-29 37.3825 37.0 37.0 40.0 33.0 40.0 30-31 37.33525 37.0 37.0 40.0 33.0 40.0 32-33 37.2275 37.0 37.0 40.0 33.0 40.0 34-35 37.039500000000004 37.0 37.0 40.0 33.0 40.0 36-37 36.983375 37.0 37.0 40.0 33.0 40.0 38-39 36.83525 37.0 37.0 40.0 33.0 40.0 40-41 36.738125 37.0 37.0 40.0 33.0 40.0 42-43 36.602625 37.0 37.0 40.0 33.0 40.0 44-45 36.381375000000006 37.0 37.0 40.0 33.0 40.0 46-47 36.285375 37.0 37.0 38.5 33.0 40.0 48-49 36.05975 37.0 37.0 37.0 33.0 40.0 50-51 35.924375 37.0 37.0 37.0 33.0 40.0 52-53 35.79075 37.0 35.0 37.0 33.0 40.0 54-55 35.58975 37.0 33.0 37.0 33.0 40.0 56-57 35.48175 37.0 33.0 37.0 33.0 37.0 58-59 35.078875 37.0 33.0 37.0 33.0 37.0 60-61 35.004 37.0 33.0 37.0 33.0 37.0 62-63 34.94125 37.0 33.0 37.0 33.0 37.0 64-65 34.500875 37.0 33.0 37.0 30.0 37.0 66-67 34.447375 37.0 33.0 37.0 33.0 37.0 68-69 33.14875 35.0 33.0 35.0 30.0 37.0 70-71 33.547538267875126 33.0 33.0 37.0 27.0 37.0 72-73 34.25230189432317 37.0 33.0 37.0 33.0 37.0 74-75 34.52954562869938 37.0 33.0 37.0 33.0 37.0 76-77 34.310267567594394 37.0 33.0 37.0 33.0 37.0 78-79 34.39116617740633 37.0 33.0 37.0 33.0 37.0 80-81 34.267375201818574 37.0 33.0 37.0 33.0 37.0 82-83 34.073014615995106 37.0 33.0 37.0 30.0 37.0 84-85 34.036790589914204 37.0 33.0 37.0 33.0 37.0 86-87 33.98783712321523 37.0 33.0 37.0 30.0 37.0 88-89 33.908381808566894 37.0 33.0 37.0 27.0 37.0 90-91 33.817292437863564 35.0 33.0 37.0 27.0 37.0 92-93 33.630222104706505 35.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 8.0 22 11.0 23 12.0 24 8.0 25 21.0 26 24.0 27 35.0 28 34.0 29 55.0 30 58.0 31 98.0 32 134.0 33 167.0 34 267.0 35 474.0 36 981.0 37 1109.0 38 492.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 88.075 2.675 2.325 6.925000000000001 2 71.48936170212767 16.896120150187734 7.334167709637046 4.280350438047559 3 35.85 38.425 13.825000000000001 11.899999999999999 4 34.599999999999994 27.900000000000002 17.849999999999998 19.650000000000002 5 25.45 31.35 24.375 18.825 6 20.4 36.725 25.525 17.349999999999998 7 34.0 30.075000000000003 20.325 15.6 8 30.5 29.625 23.849999999999998 16.025 9 27.474999999999998 26.5 27.525 18.5 10-11 25.474999999999998 27.787499999999998 28.175 18.5625 12-13 29.4375 24.575 27.900000000000002 18.087500000000002 14-15 23.6375 27.950000000000003 28.812500000000004 19.6 16-17 24.65 30.0875 26.087500000000002 19.175 18-19 24.175 26.400000000000002 26.737499999999997 22.6875 20-21 24.7875 25.7 27.250000000000004 22.2625 22-23 26.437500000000004 24.087500000000002 27.3875 22.0875 24-25 27.6 23.575 27.5625 21.2625 26-27 25.112499999999997 26.275 28.962500000000002 19.650000000000002 28-29 26.075 25.8625 27.037499999999998 21.025 30-31 26.025 24.7875 28.375 20.8125 32-33 24.3125 27.037499999999998 27.962500000000002 20.6875 34-35 26.224999999999998 23.95 28.3875 21.4375 36-37 24.0375 24.375 29.325000000000003 22.2625 38-39 25.724999999999998 25.412499999999998 29.675 19.1875 40-41 25.7375 24.95 27.437499999999996 21.875 42-43 24.3 26.974999999999998 27.750000000000004 20.974999999999998 44-45 23.7 25.874999999999996 28.15 22.275 46-47 24.175 23.4625 29.7125 22.650000000000002 48-49 24.55 25.4375 29.5875 20.424999999999997 50-51 25.2125 26.0125 29.675 19.1 52-53 25.174999999999997 26.674999999999997 28.199999999999996 19.950000000000003 54-55 22.45 28.849999999999998 28.775000000000002 19.925 56-57 25.2625 24.1875 29.775000000000002 20.775 58-59 23.8375 24.4875 29.8875 21.7875 60-61 24.2625 25.15 30.075000000000003 20.5125 62-63 22.7 27.125 30.887500000000003 19.287499999999998 64-65 24.9375 25.4875 29.549999999999997 20.025000000000002 66-67 25.5375 26.787499999999998 28.000000000000004 19.675 68-69 24.1625 26.75 28.6875 20.4 70-71 25.413948820873056 24.824385348720522 29.440541896638234 20.321123933768188 72-73 24.860900354071827 24.69650986342944 29.299443601416286 21.143146181082447 74-75 23.386378103119032 26.556333545512413 29.471674092934435 20.585614258434116 76-77 22.756410256410255 25.756410256410255 31.025641025641026 20.46153846153846 78-79 24.250258531540847 25.0129265770424 30.739400206825234 19.99741468459152 80-81 23.571521541064687 27.18989977873227 29.376545620200446 19.862033060002602 82-83 23.615656499541828 24.820002618143736 30.422830213378717 21.141510668935727 84-85 25.026385224274406 24.894459102902374 29.947229551451187 20.131926121372032 86-87 23.016922263352726 26.427815970386035 30.883130618720255 19.672131147540984 88-89 22.355896351136963 27.815970386039133 31.22686409307245 18.601269169751454 90-91 25.105764145954524 26.83765203595981 28.34479111581174 19.71179270227393 92-93 22.461660497091486 29.455314648334213 29.375991538868323 18.707033315705974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 0.0 20 1.5 21 3.0 22 5.0 23 5.5 24 4.0 25 4.5 26 8.5 27 12.0 28 14.5 29 19.5 30 27.5 31 37.5 32 49.5 33 57.5 34 68.0 35 92.5 36 123.0 37 144.0 38 164.0 39 174.5 40 181.0 41 197.5 42 207.5 43 209.5 44 206.5 45 201.5 46 203.5 47 195.5 48 178.0 49 160.0 50 143.0 51 159.0 52 160.5 53 151.5 54 135.0 55 85.0 56 61.5 57 64.0 58 65.0 59 62.5 60 51.0 61 41.0 62 35.5 63 29.0 64 33.5 65 29.5 66 22.5 67 20.5 68 13.5 69 11.0 70 7.0 71 5.0 72 4.0 73 3.0 74 2.5 75 1.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.013190871916633689 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 28.0 71 12.0 72 12.0 73 15.0 74 11.0 75 15.0 76 14.0 77 17.0 78 16.0 79 11.0 80 15.0 81 8.0 82 13.0 83 14.0 84 17.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3782.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.02499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 94.71416259695489 82.425 2 2.987647227808101 5.2 3 1.2640045963803506 3.3000000000000003 4 0.2872737719046251 1.0 5 0.25854639471416263 1.125 6 0.028727377190462512 0.15 7 0.028727377190462512 0.17500000000000002 8 0.028727377190462512 0.2 9 0.0 0.0 >10 0.4021832806664752 6.425 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 40 1.0 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 35 0.8750000000000001 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 30 0.75 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 25 0.625 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 19 0.475 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 18 0.44999999999999996 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 14 0.35000000000000003 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 12 0.3 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 12 0.3 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 11 0.27499999999999997 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 11 0.27499999999999997 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 10 0.25 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 10 0.25 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 10 0.25 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 8 0.2 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 7 0.17500000000000002 No Hit GGAGAACGGCGGCTGCGGCTGCAGCACCTGCAAGTGCGGCACCAGCTGCG 6 0.15 No Hit GGAGTCCCATATATATATGTATAAGATGCCAGCCCGGTTTCGTCAACCAT 5 0.125 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 5 0.125 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 5 0.125 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 5 0.125 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 5 0.125 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 5 0.125 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 5 0.125 No Hit GGGTGTGAGCTGGAGAGACGATCGGGTCTCTCAGCCGGCGTCTTCATCAG 5 0.125 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.05 0.0 0.0 0.0 0.0 10-11 0.05 0.0 0.0 0.0 0.0 12-13 0.05 0.0 0.0 0.0 0.0 14-15 0.05 0.0 0.0 0.0 0.0 16-17 0.05 0.0 0.0 0.0 0.0 18-19 0.05 0.0 0.0 0.0 0.0 20-21 0.05 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.075 0.0 0.0 0.0 0.0 32-33 0.075 0.0 0.0 0.0 0.0 34-35 0.075 0.0 0.0 0.0 0.0 36-37 0.075 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.0875 0.0 0.0 0.0 0.0 46-47 0.125 0.0 0.0 0.0 0.0 48-49 0.125 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra Rejected 199301 READS because READLEN < 1 Read 199301 spots for ERR6133444.sra Written 199301 spots for ERR6133444.sra Rejected 199299 READS because READLEN < 1 Read 199299 spots for ERR6133444.sra Written 199299 spots for ERR6133444.sra SRR ids: ['ERR6133444.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9poo0rwr ERR6133444.sra spots: 3985982 blocks: [[1, 199299], [199300, 398598], [398599, 597897], [597898, 797196], [797197, 996495], [996496, 1195794], [1195795, 1395093], [1395094, 1594392], [1594393, 1793691], [1793692, 1992990], [1992991, 2192289], [2192290, 2391588], [2391589, 2590887], [2590888, 2790186], [2790187, 2989485], [2989486, 3188784], [3188785, 3388083], [3388084, 3587382], [3587383, 3786681], [3786682, 3985982]] ERR6133444 file size 877730 ERR6133444 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133444 ERR6133444_1.fastq Input file: ERR6133444_1.fastq trimmed: ERR6133444-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:07:57 2024 >> started Sat Dec 7 06:07:59 2024 >> done (2.539s) 3985982 reads processed; of these: 1862 ( 0.05%) short reads filtered out after trimming by size control 49 ( 0.00%) empty reads filtered out after trimming by size control 3984071 (99.95%) reads available; of these: 48176 ( 1.21%) trimmed reads available after processing 3935895 (98.79%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 43 0.00% 19 57 0.00% 20 39 0.00% 21 23 0.00% 22 17 0.00% 23 26 0.00% 24 12 0.00% 25 9 0.00% 26 10 0.00% 27 7 0.00% 28 16 0.00% 29 189 0.00% 30 13 0.00% 31 9 0.00% 32 27 0.00% 33 12 0.00% 34 8 0.00% 35 9 0.00% 36 10 0.00% 37 10 0.00% 38 27 0.00% 39 53 0.00% 40 81 0.00% 41 30 0.00% 42 30 0.00% 43 21 0.00% 44 29 0.00% 45 24 0.00% 46 28 0.00% 47 20 0.00% 48 18 0.00% 49 29 0.00% 50 26 0.00% 51 59 0.00% 52 30 0.00% 53 24 0.00% 54 38 0.00% 55 30 0.00% 56 26 0.00% 57 24 0.00% 58 22 0.00% 59 24 0.00% 60 23 0.00% 61 29 0.00% 62 2 0.00% 63 3 0.00% 64 3 0.00% 65 4 0.00% 66 12 0.00% 67 18 0.00% 68 32 0.00% 69 158 0.00% 70 16458 0.41% 71 15621 0.39% 72 16419 0.41% 73 15453 0.39% 74 15994 0.40% 75 16780 0.42% 76 15824 0.40% 77 15070 0.38% 78 16053 0.40% 79 16770 0.42% 80 15586 0.39% 81 16073 0.40% 82 17233 0.43% 83 18167 0.46% 84 15269 0.38% 85 107 0.00% 86 167 0.00% 87 285 0.01% 88 541 0.01% 89 1121 0.03% 90 2542 0.06% 91 7568 0.19% 92 32050 0.80% 93 3695397 92.75% 3984071 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=34 prefix-density=0.30 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.05 sequence-density-rank=20 fanout-score=99.59 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=21.0 sequence=TTTTTTTTTTAAGAATCCTCCATTTTTGTTCTTCCACCCATGCAATAGAGAGCAAATGGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGAGTCATGGAATAATGGTGAATTCAAATGTTTAGTTCTTTAGTATAAGAAGTATAAGAA Started job on | Dec 07 06:08:16 Started mapping on | Dec 07 06:08:16 Finished on | Dec 07 06:08:24 Mapping speed, Million of reads per hour | 1792.83 Number of input reads | 3984071 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 3195881 Uniquely mapped reads % | 80.22% Average mapped length | 91.59 Number of splices: Total | 170727 Number of splices: Annotated (sjdb) | 140173 Number of splices: GT/AG | 164726 Number of splices: GC/AG | 3251 Number of splices: AT/AC | 42 Number of splices: Non-canonical | 2708 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.04% Deletion average length | 1.67 Insertion rate per base | 0.02% Insertion average length | 1.96 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 704142 % of reads mapped to multiple loci | 17.67% Number of reads mapped to too many loci | 20219 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.57% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 84048 84048 84048 N_multimapping 704142 704142 704142 N_noFeature 182208 212910 3054189 N_ambiguous 122716 11756 467 UnstrandedReadsAssigned:2890957 PositiveStrandReadsAssigned:2971215 NegativeStrandReadsAssigned:141225 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133444 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133444-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,984,071 reads, 3,479,846 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,035 rounds 52973 ERR6133444.ke.tsv 35125 ERR6133444.se.tsv 88098 total ==> ERR6133444.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 122 34.9965 PNS24243 293 194 0 0 KQK14069 1603 1504 81 21.1961 KQK14071 474 375 0 0 ==> ERR6133444.se.tsv <== BRADI_1g14170v3 81 BRADI_1g53295v3 75 BRADI_1g59795v3 38 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 34 BRADI_1g74790v3 31 BRADI_1g09890v3 0 BRADI_1g77505v3 92 BRADI_1g48960v3 0 ERR6133444 completed mapping pipeline successfully