Starting /dee2/code/volunteer_pipeline.sh ERR6133445 current disk space = 1545682575360 free memory = 1451267896 ERR6133445 SRAfilesize e43284828cb88d05d291f2d1a710ac20 ERR6133445.sra ERR6133445.sra file validated ERR6133445 is single end ERR6133445 is conventional basespace ERR6133445 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133445_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.2345 37.0 33.0 37.0 33.0 37.0 2 36.4435 37.0 37.0 37.0 37.0 37.0 3 35.73225 37.0 37.0 37.0 33.0 37.0 4 35.364 37.0 37.0 37.0 33.0 37.0 5 35.215 37.0 37.0 37.0 33.0 37.0 6 35.6285 37.0 37.0 37.0 33.0 37.0 7 37.31575 37.0 37.0 40.0 33.0 40.0 8 37.281 37.0 37.0 40.0 33.0 40.0 9 37.26425 37.0 37.0 40.0 33.0 40.0 10-11 37.208375000000004 37.0 37.0 40.0 33.0 40.0 12-13 37.10325 37.0 37.0 40.0 33.0 40.0 14-15 37.076875 37.0 37.0 40.0 33.0 40.0 16-17 36.949 37.0 37.0 40.0 33.0 40.0 18-19 37.19225 37.0 37.0 40.0 33.0 40.0 20-21 37.31875 37.0 37.0 40.0 33.0 40.0 22-23 37.223 37.0 37.0 40.0 33.0 40.0 24-25 37.2415 37.0 37.0 40.0 33.0 40.0 26-27 36.94425 37.0 37.0 40.0 33.0 40.0 28-29 37.088 37.0 37.0 40.0 33.0 40.0 30-31 37.022375 37.0 37.0 40.0 33.0 40.0 32-33 36.84475 37.0 37.0 40.0 33.0 40.0 34-35 36.671125 37.0 37.0 40.0 33.0 40.0 36-37 36.6035 37.0 37.0 40.0 33.0 40.0 38-39 36.51325 37.0 37.0 40.0 33.0 40.0 40-41 36.356 37.0 37.0 40.0 33.0 40.0 42-43 36.223 37.0 37.0 40.0 33.0 40.0 44-45 35.953875 37.0 35.0 37.0 33.0 40.0 46-47 35.951750000000004 37.0 35.0 37.0 33.0 40.0 48-49 35.788375 37.0 35.0 37.0 33.0 40.0 50-51 35.65 37.0 33.0 37.0 33.0 40.0 52-53 35.473749999999995 37.0 33.0 37.0 33.0 40.0 54-55 35.42425 37.0 33.0 37.0 33.0 37.0 56-57 35.291375 37.0 33.0 37.0 33.0 37.0 58-59 34.81875 37.0 33.0 37.0 33.0 37.0 60-61 34.710125000000005 37.0 33.0 37.0 33.0 37.0 62-63 34.672 37.0 33.0 37.0 33.0 37.0 64-65 34.288624999999996 37.0 33.0 37.0 30.0 37.0 66-67 34.26075 37.0 33.0 37.0 30.0 37.0 68-69 32.932625 35.0 33.0 35.0 27.0 37.0 70-71 33.26650281602002 33.0 33.0 37.0 27.0 37.0 72-73 34.09470137836401 37.0 33.0 37.0 30.0 37.0 74-75 34.30630684743382 37.0 33.0 37.0 33.0 37.0 76-77 34.127783841420865 37.0 33.0 37.0 27.0 37.0 78-79 34.14302011024712 37.0 33.0 37.0 30.0 37.0 80-81 34.12775209562295 37.0 33.0 37.0 30.0 37.0 82-83 33.78920582864586 37.0 33.0 37.0 27.0 37.0 84-85 33.822785823624066 37.0 33.0 37.0 27.0 37.0 86-87 33.746417604913 35.0 33.0 37.0 27.0 37.0 88-89 33.67003582395087 35.0 33.0 37.0 27.0 37.0 90-91 33.59698055271238 33.0 33.0 37.0 27.0 37.0 92-93 33.252942681678604 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 7.0 21 17.0 22 15.0 23 14.0 24 18.0 25 25.0 26 23.0 27 33.0 28 42.0 29 57.0 30 88.0 31 80.0 32 132.0 33 185.0 34 282.0 35 578.0 36 1001.0 37 1044.0 38 351.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 90.05 2.025 2.0500000000000003 5.875 2 76.20715536652489 14.410808106079559 5.529146860145109 3.852889667250438 3 38.375 38.324999999999996 12.3 11.0 4 36.55 26.700000000000003 16.7 20.05 5 25.275 33.0 23.175 18.55 6 21.85 37.55 24.2 16.400000000000002 7 36.85 29.799999999999997 16.8 16.55 8 31.35 29.049999999999997 24.025 15.575 9 28.849999999999998 25.825 27.325 18.0 10-11 27.6625 26.1125 25.2625 20.962500000000002 12-13 31.674999999999997 23.7875 24.5125 20.025000000000002 14-15 23.8625 28.7 28.012500000000003 19.425 16-17 25.45 31.225 24.325 19.0 18-19 23.7625 27.175 24.05 25.0125 20-21 25.55 26.950000000000003 26.25 21.25 22-23 27.750000000000004 22.9625 26.187500000000004 23.1 24-25 29.7375 22.3 25.5375 22.425 26-27 27.125 25.5375 27.875 19.4625 28-29 27.987499999999997 26.1 23.925 21.987499999999997 30-31 29.425 23.9 23.9875 22.6875 32-33 24.95 29.1625 24.125 21.762500000000003 34-35 27.800000000000004 23.075000000000003 26.0625 23.0625 36-37 24.5375 23.7 28.925 22.8375 38-39 26.187500000000004 25.0125 28.787499999999998 20.0125 40-41 28.012500000000003 25.5 24.2625 22.225 42-43 25.7375 28.4125 23.6125 22.237499999999997 44-45 25.15 25.3125 25.5125 24.025 46-47 25.162499999999998 22.95 26.4125 25.474999999999998 48-49 26.087500000000002 24.087500000000002 28.675 21.15 50-51 28.3625 25.35 26.724999999999998 19.5625 52-53 26.700000000000003 27.8375 24.712500000000002 20.75 54-55 24.025 29.8375 25.025 21.1125 56-57 26.637499999999996 25.0375 26.2875 22.037499999999998 58-59 24.8125 24.3 26.85 24.0375 60-61 25.85 22.912499999999998 27.3125 23.925 62-63 23.674999999999997 27.462500000000002 30.012499999999996 18.85 64-65 25.9625 25.924999999999997 26.387500000000003 21.725 66-67 26.2125 26.450000000000003 25.95 21.3875 68-69 25.4875 26.724999999999998 25.6125 22.175 70-71 26.604127579737337 25.465916197623518 25.215759849906195 22.71419637273296 72-73 26.9717868338558 23.73667711598746 25.918495297805645 23.373040752351095 74-75 23.68024132730015 28.192559074912015 27.136752136752136 20.990447461035696 76-77 22.639606706164127 26.219589058363795 28.475986385982605 22.664817849489474 78-79 25.037917087967642 22.914560161779576 28.804347826086957 23.243174924165825 80-81 25.392802838317287 25.924987328940702 28.71262037506336 19.969589457678662 82-83 25.712468193384225 22.748091603053435 29.12213740458015 22.41730279898219 84-85 25.85677749360614 22.58312020460358 28.54219948849105 23.017902813299234 86-87 23.208802456499487 24.83367451381781 32.90685772773798 19.05066530194473 88-89 23.669396110542475 29.183725690890483 28.28812691914023 18.858751279426816 90-91 27.546059365404297 24.744114636642784 27.40532241555783 20.304503582395085 92-93 24.52661207778915 28.53121801432958 27.37973387922211 19.56243602865916 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.5 18 2.0 19 1.0 20 2.0 21 2.0 22 4.0 23 5.0 24 2.0 25 3.5 26 6.0 27 11.0 28 16.5 29 17.5 30 21.5 31 27.5 32 28.5 33 36.5 34 46.0 35 51.5 36 69.5 37 89.5 38 108.5 39 122.5 40 132.0 41 152.0 42 162.0 43 158.5 44 160.5 45 160.0 46 237.0 47 249.0 48 171.0 49 165.0 50 169.0 51 164.0 52 166.0 53 202.0 54 168.5 55 104.5 56 97.5 57 92.0 58 85.0 59 79.5 60 77.0 61 74.5 62 67.0 63 57.0 64 56.0 65 52.5 66 48.0 67 38.0 68 24.0 69 18.5 70 11.5 71 5.0 72 3.5 73 3.0 74 2.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.075 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.03834845967020325 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 5.0 71 6.0 72 3.0 73 7.0 74 2.0 75 8.0 76 5.0 77 6.0 78 4.0 79 5.0 80 6.0 81 10.0 82 6.0 83 12.0 84 7.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3908.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.525 #Duplication Level Percentage of deduplicated Percentage of total 1 92.82831418814033 74.75 2 4.43961502638932 7.1499999999999995 3 1.3970816516609748 3.375 4 0.40360136603539276 1.3 5 0.18627755355479667 0.75 6 0.18627755355479667 0.8999999999999999 7 0.09313877677739833 0.525 8 0.03104625892579944 0.2 9 0.03104625892579944 0.22499999999999998 >10 0.34150884818379384 6.425 >50 0.06209251785159888 4.3999999999999995 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 91 2.275 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 85 2.125 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 39 0.975 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 37 0.9249999999999999 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 37 0.9249999999999999 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 36 0.8999999999999999 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 29 0.7250000000000001 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 18 0.44999999999999996 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 14 0.35000000000000003 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 12 0.3 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 12 0.3 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 12 0.3 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 11 0.27499999999999997 No Hit GGGCAAGGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAA 9 0.22499999999999998 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 8 0.2 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 7 0.17500000000000002 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 7 0.17500000000000002 No Hit GGGGAAGAAGACCTCTTTCTGGGAGGCCGAAGCCACTTCGGCACCGGCAC 7 0.17500000000000002 No Hit GGAGTCCCATATATATATGTATAAGATGCCAGCCCGGTTTCGTCAACCAT 6 0.15 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 6 0.15 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 6 0.15 No Hit GGAACAAACGAGGATAAGATAAAATTGCTTTAAATTTATTTTGCCCAAGT 6 0.15 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 6 0.15 No Hit GGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTG 6 0.15 No Hit GGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTT 5 0.125 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 5 0.125 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 5 0.125 No Hit GGACTGGGTATCCATGCCAGGTGTTATACCAGTAGCTTCAGGTGGTATTC 5 0.125 No Hit GGATTCTTCTTTTTTGTGGGCCATTTGTGGCATGCAGGAAGAGCCCGAGC 5 0.125 No Hit GGGCAATGTCAAACCAGAAGACGTCTTCCAGACGGTTTCCAAGACGGGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.037500000000000006 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0125 58-59 0.05 0.0 0.0 0.0 0.025 60-61 0.05 0.0 0.0 0.0 0.025 62-63 0.05 0.0 0.0 0.0 0.025 64-65 0.05 0.0 0.0 0.0 0.025 66-67 0.05 0.0 0.0 0.0 0.025 68-69 0.05 0.0 0.0 0.0 0.025 70-71 0.05 0.0 0.0 0.0 0.025 72-73 0.05 0.0 0.0 0.0 0.025 74-75 0.05 0.0 0.0 0.0 0.025 76-77 0.05 0.0 0.0 0.0 0.025 78-79 0.05 0.0 0.0 0.0 0.025 80-81 0.05 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207306 READS because READLEN < 1 Read 207306 spots for ERR6133445.sra Written 207306 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra Rejected 207292 READS because READLEN < 1 Read 207292 spots for ERR6133445.sra Written 207292 spots for ERR6133445.sra SRR ids: ['ERR6133445.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_13znrxrj ERR6133445.sra spots: 4145854 blocks: [[1, 207292], [207293, 414584], [414585, 621876], [621877, 829168], [829169, 1036460], [1036461, 1243752], [1243753, 1451044], [1451045, 1658336], [1658337, 1865628], [1865629, 2072920], [2072921, 2280212], [2280213, 2487504], [2487505, 2694796], [2694797, 2902088], [2902089, 3109380], [3109381, 3316672], [3316673, 3523964], [3523965, 3731256], [3731257, 3938548], [3938549, 4145854]] ERR6133445 file size 917648 ERR6133445 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133445 ERR6133445_1.fastq Input file: ERR6133445_1.fastq trimmed: ERR6133445-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:10:35 2024 >> started Sat Dec 7 06:10:40 2024 >> done (4.894s) 4145854 reads processed; of these: 2047 ( 0.05%) short reads filtered out after trimming by size control 18 ( 0.00%) empty reads filtered out after trimming by size control 4143789 (99.95%) reads available; of these: 64300 ( 1.55%) trimmed reads available after processing 4079489 (98.45%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 35 0.00% 19 37 0.00% 20 33 0.00% 21 14 0.00% 22 22 0.00% 23 21 0.00% 24 13 0.00% 25 11 0.00% 26 5 0.00% 27 11 0.00% 28 3 0.00% 29 53 0.00% 30 11 0.00% 31 5 0.00% 32 13 0.00% 33 11 0.00% 34 15 0.00% 35 14 0.00% 36 8 0.00% 37 10 0.00% 38 27 0.00% 39 30 0.00% 40 43 0.00% 41 24 0.00% 42 28 0.00% 43 29 0.00% 44 26 0.00% 45 31 0.00% 46 20 0.00% 47 26 0.00% 48 29 0.00% 49 33 0.00% 50 42 0.00% 51 48 0.00% 52 28 0.00% 53 41 0.00% 54 28 0.00% 55 29 0.00% 56 23 0.00% 57 32 0.00% 58 36 0.00% 59 24 0.00% 60 28 0.00% 61 12 0.00% 62 5 0.00% 63 3 0.00% 64 4 0.00% 65 7 0.00% 66 6 0.00% 67 6 0.00% 68 29 0.00% 69 81 0.00% 70 7799 0.19% 71 6409 0.15% 72 7076 0.17% 73 6174 0.15% 74 6665 0.16% 75 6588 0.16% 76 5762 0.14% 77 5775 0.14% 78 6776 0.16% 79 7877 0.19% 80 6843 0.17% 81 7260 0.18% 82 8224 0.20% 83 9125 0.22% 84 6928 0.17% 85 128 0.00% 86 228 0.01% 87 376 0.01% 88 798 0.02% 89 1557 0.04% 90 3723 0.09% 91 11054 0.27% 92 43836 1.06% 93 3975605 95.94% 4143789 reads passed initial QC criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=5.50 fanout-score-rank=22 prefix-density=1.18 prefix-fanout=2.5 sequence=GCCGCCGCCGCCAAGGAAGGCATGTTCGTCAAGAACTACAGCTACTGATCCTAATCGCATCAAGCTTCAACGCCTGTGAGTGAAAACCAGTGATGAGAGTGCTGCTGCTAGCTAGCGCCGGCATTGATGAGCTTGAGAGGGCACTGTAGCCAGTGTGTCAGTCGTTGTTAAATTACAGGTTGAGATCATCAGCGTACTCCGATGGGAGATGGACATCAGAAAGTATACTGTGTTTTACCACCCTAATTAAGTAAACAACTTTTGGAATGGTGATTATCTTACTGTTTTAAATAAATCTGTTTC criterion=fanout-score sequence-density=0.15 sequence-density-rank=6 fanout-score=60.75 fanout-score-rank=1 prefix-density=0.77 prefix-fanout=11.7 sequence=GAAGAAGAAGAAACGCATGGTGCCCTGCTTCCGTCTGTCGGCTGCTTGCTTGGCAACGGCAGAGCAGAGCTTGGTGCAGTAAAACTACTGGTTATACTCTCTGTATGTAAAGTTAAAATTTTCACACACAGCTATGTGCTAAAGGA Started job on | Dec 07 06:11:00 Started mapping on | Dec 07 06:11:01 Finished on | Dec 07 06:11:23 Mapping speed, Million of reads per hour | 678.07 Number of input reads | 4143789 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 3037080 Uniquely mapped reads % | 73.29% Average mapped length | 92.14 Number of splices: Total | 271309 Number of splices: Annotated (sjdb) | 235051 Number of splices: GT/AG | 265820 Number of splices: GC/AG | 3487 Number of splices: AT/AC | 70 Number of splices: Non-canonical | 1932 Mismatch rate per base, % | 0.56% Deletion rate per base | 0.04% Deletion average length | 1.85 Insertion rate per base | 0.03% Insertion average length | 2.29 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1013405 % of reads mapped to multiple loci | 24.46% Number of reads mapped to too many loci | 12323 % of reads mapped to too many loci | 0.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.94% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 93304 93304 93304 N_multimapping 1013405 1013405 1013405 N_noFeature 130449 173070 2889866 N_ambiguous 115738 11405 377 UnstrandedReadsAssigned:2790893 PositiveStrandReadsAssigned:2852605 NegativeStrandReadsAssigned:146837 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133445 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133445-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 4,143,789 reads, 3,692,306 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,185 rounds 52973 ERR6133445.ke.tsv 35125 ERR6133445.se.tsv 88098 total ==> ERR6133445.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 102 26.224 PNS24243 293 194 0 0 KQK14069 1603 1504 29 6.80146 KQK14071 474 375 0 0 ==> ERR6133445.se.tsv <== BRADI_1g14170v3 29 BRADI_1g53295v3 92 BRADI_1g59795v3 14 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 62 BRADI_1g74790v3 70 BRADI_1g09890v3 0 BRADI_1g77505v3 56 BRADI_1g48960v3 0 ERR6133445 completed mapping pipeline successfully