Starting /dee2/code/volunteer_pipeline.sh ERR6133447 current disk space = 1545642131456 free memory = 1603228992 ERR6133447 SRAfilesize 5dc3ef09aa0bec884cddfe642e7947da ERR6133447.sra ERR6133447.sra file validated ERR6133447 is single end ERR6133447 is conventional basespace ERR6133447 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133447_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.2445 37.0 33.0 37.0 33.0 37.0 2 36.4045 37.0 37.0 37.0 33.0 37.0 3 35.76825 37.0 37.0 37.0 33.0 37.0 4 35.46075 37.0 37.0 37.0 33.0 37.0 5 35.3535 37.0 37.0 37.0 33.0 37.0 6 35.7055 37.0 37.0 37.0 33.0 37.0 7 37.265 37.0 37.0 40.0 33.0 40.0 8 37.33825 37.0 37.0 40.0 33.0 40.0 9 37.38525 37.0 37.0 40.0 33.0 40.0 10-11 37.29625 37.0 37.0 40.0 33.0 40.0 12-13 37.219625 37.0 37.0 40.0 33.0 40.0 14-15 37.09025 37.0 37.0 40.0 33.0 40.0 16-17 36.968625 37.0 37.0 40.0 33.0 40.0 18-19 37.062625 37.0 37.0 40.0 33.0 40.0 20-21 37.321125 37.0 37.0 40.0 33.0 40.0 22-23 36.957125000000005 37.0 37.0 40.0 33.0 40.0 24-25 37.115125 37.0 37.0 40.0 33.0 40.0 26-27 37.0215 37.0 37.0 40.0 33.0 40.0 28-29 37.1815 37.0 37.0 40.0 33.0 40.0 30-31 37.137625 37.0 37.0 40.0 33.0 40.0 32-33 36.990875 37.0 37.0 40.0 33.0 40.0 34-35 36.768625 37.0 37.0 40.0 33.0 40.0 36-37 36.7915 37.0 37.0 40.0 33.0 40.0 38-39 36.555875 37.0 37.0 40.0 33.0 40.0 40-41 36.405375 37.0 37.0 40.0 33.0 40.0 42-43 36.374875 37.0 37.0 40.0 33.0 40.0 44-45 36.119 37.0 37.0 38.5 33.0 40.0 46-47 35.99125 37.0 37.0 37.0 33.0 40.0 48-49 35.74975 37.0 35.0 37.0 33.0 40.0 50-51 35.64 37.0 33.0 37.0 33.0 40.0 52-53 35.562250000000006 37.0 33.0 37.0 33.0 40.0 54-55 35.338375 37.0 33.0 37.0 33.0 37.0 56-57 35.208 37.0 33.0 37.0 33.0 37.0 58-59 34.73175 37.0 33.0 37.0 30.0 37.0 60-61 34.647125 37.0 33.0 37.0 33.0 37.0 62-63 34.79275 37.0 33.0 37.0 33.0 37.0 64-65 34.318 37.0 33.0 37.0 27.0 37.0 66-67 34.23025 37.0 33.0 37.0 30.0 37.0 68-69 32.95425 35.0 33.0 35.0 27.0 37.0 70-71 33.15278172811638 33.0 33.0 37.0 27.0 37.0 72-73 33.97002883360534 37.0 33.0 37.0 27.0 37.0 74-75 34.325002582342464 37.0 33.0 37.0 33.0 37.0 76-77 34.225370589900116 37.0 33.0 37.0 30.0 37.0 78-79 34.12105945401896 37.0 33.0 37.0 30.0 37.0 80-81 34.055091932569034 37.0 33.0 37.0 27.0 37.0 82-83 33.875343368535795 37.0 33.0 37.0 27.0 37.0 84-85 33.915796250253955 37.0 33.0 37.0 27.0 37.0 86-87 33.737967229902715 37.0 33.0 37.0 27.0 37.0 88-89 33.769329237071176 37.0 33.0 37.0 27.0 37.0 90-91 33.675499231950845 35.0 33.0 37.0 27.0 37.0 92-93 33.37775217613927 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 17.0 22 15.0 23 13.0 24 25.0 25 20.0 26 22.0 27 51.0 28 49.0 29 58.0 30 75.0 31 90.0 32 145.0 33 174.0 34 256.0 35 466.0 36 1047.0 37 1080.0 38 392.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 91.175 1.95 1.775 5.1 2 76.21905476369092 14.753688422105526 5.1512878219554885 3.875968992248062 3 37.65 38.75 12.8 10.8 4 36.55 27.500000000000004 16.7 19.25 5 24.349999999999998 33.650000000000006 23.0 19.0 6 20.8 38.375 24.25 16.575 7 36.425000000000004 28.925 17.925 16.725 8 32.300000000000004 31.175000000000004 22.125 14.399999999999999 9 28.175 27.55 25.3 18.975 10-11 26.5625 28.012500000000003 25.624999999999996 19.8 12-13 29.6875 24.3625 27.3 18.65 14-15 24.375 28.825 28.1125 18.6875 16-17 24.6625 30.85 25.937500000000004 18.55 18-19 24.7 25.5 26.4625 23.3375 20-21 24.837500000000002 26.2125 26.987499999999997 21.9625 22-23 26.5125 23.6125 26.4625 23.4125 24-25 26.974999999999998 24.474999999999998 27.675 20.875 26-27 24.9 25.4375 30.4625 19.2 28-29 26.450000000000003 26.35 26.200000000000003 21.0 30-31 27.375 25.35 25.624999999999996 21.65 32-33 25.2 27.462500000000002 25.9875 21.349999999999998 34-35 26.75 23.4125 27.05 22.787499999999998 36-37 24.75 23.200000000000003 29.175 22.875 38-39 25.137500000000003 25.887500000000003 30.062499999999996 18.912499999999998 40-41 28.249999999999996 24.837500000000002 25.1 21.8125 42-43 25.3125 27.762500000000003 26.0375 20.8875 44-45 24.2875 25.112499999999997 28.3625 22.237499999999997 46-47 24.1125 23.05 28.299999999999997 24.5375 48-49 25.4 24.45 30.837500000000002 19.3125 50-51 27.1375 25.224999999999998 28.449999999999996 19.1875 52-53 26.2125 27.450000000000003 25.8 20.5375 54-55 23.1125 29.012500000000003 27.6625 20.2125 56-57 25.3125 25.337500000000002 28.9 20.45 58-59 24.7875 24.0125 28.65 22.55 60-61 24.2875 23.7375 29.325000000000003 22.650000000000002 62-63 21.775 27.9125 31.374999999999996 18.9375 64-65 23.9875 25.337500000000002 29.762499999999996 20.9125 66-67 25.8625 25.224999999999998 28.537499999999998 20.375 68-69 23.3375 26.0625 28.8375 21.762500000000003 70-71 25.87955427569801 24.22686866157506 28.095655440090145 21.797921622636785 72-73 26.20871530830089 23.98593494913977 27.84126585457742 21.964083887981918 74-75 23.488664987405542 26.33501259445844 29.584382871536523 20.591939546599498 76-77 23.02149178255373 25.70164348925411 29.88621997471555 21.39064475347661 78-79 23.50481500253421 24.39178915357324 30.245818550430815 21.857577293461734 80-81 23.73698908352374 26.415333841076418 30.33764914953034 19.51002792586951 82-83 24.7992863514719 23.371989295272076 31.298585446667516 20.530138906588505 84-85 25.095981571538267 23.137957512157666 30.176606091630408 21.589454824673663 86-87 23.41269841269841 25.3584229390681 32.565284178187405 18.663594470046082 88-89 21.85099846390169 28.865847414234512 30.93958013312852 18.34357398873528 90-91 25.793650793650798 25.47363031233999 28.609831029185866 20.12288786482335 92-93 23.41269841269841 28.238607270865334 29.493087557603687 18.855606758832565 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.0 20 3.0 21 4.5 22 3.5 23 4.5 24 4.0 25 4.5 26 8.5 27 12.5 28 15.5 29 16.0 30 21.5 31 25.0 32 34.0 33 53.5 34 64.5 35 71.5 36 89.5 37 109.5 38 137.0 39 163.5 40 169.5 41 181.5 42 199.0 43 204.0 44 209.0 45 200.5 46 234.0 47 226.5 48 169.0 49 164.0 50 157.5 51 163.0 52 158.0 53 164.5 54 137.0 55 84.5 56 68.5 57 57.0 58 65.0 59 70.5 60 62.0 61 45.5 62 37.5 63 44.0 64 43.5 65 37.5 66 28.5 67 21.5 68 17.5 69 11.0 70 8.5 71 8.0 72 5.0 73 3.5 74 3.5 75 1.5 76 0.5 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.012795905310300705 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 13.0 71 3.0 72 5.0 73 5.0 74 8.0 75 9.0 76 4.0 77 4.0 78 6.0 79 3.0 80 2.0 81 8.0 82 13.0 83 8.0 84 3.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3906.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.125 #Duplication Level Percentage of deduplicated Percentage of total 1 92.75494672754947 76.175 2 4.596651445966515 7.55 3 0.9436834094368342 2.325 4 0.4870624048706241 1.6 5 0.334855403348554 1.375 6 0.182648401826484 0.8999999999999999 7 0.15220700152207 0.8750000000000001 8 0.091324200913242 0.6 9 0.030441400304414005 0.22499999999999998 >10 0.365296803652968 5.625 >50 0.06088280060882801 2.75 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 55 1.375 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 55 1.375 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 33 0.8250000000000001 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 32 0.8 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 29 0.7250000000000001 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 20 0.5 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 18 0.44999999999999996 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 17 0.42500000000000004 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 16 0.4 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 16 0.4 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 12 0.3 No Hit GGAGTCCCATATATATATGTATAAGATGCCAGCCCGGTTTCGTCAACCAT 11 0.27499999999999997 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 11 0.27499999999999997 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 10 0.25 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 9 0.22499999999999998 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 8 0.2 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 8 0.2 No Hit GGAAATGGTGAAGATGATACGAATATGCCGGCCGGGTGCTGATATTGTGA 8 0.2 No Hit GGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACGA 7 0.17500000000000002 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 7 0.17500000000000002 No Hit GGAGATGGCCGCGGAGAACGGCGGCTGCGGCTGCAGCACCTGCAAGTGCG 7 0.17500000000000002 No Hit GGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAAGATGAT 7 0.17500000000000002 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 7 0.17500000000000002 No Hit GGGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACG 6 0.15 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 6 0.15 No Hit GGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTT 6 0.15 No Hit GGGCTTGACCATGCAGCCAAGATCAAGAGCCCCGCCGAAGCCGAGAAGTA 6 0.15 No Hit GGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAG 6 0.15 No Hit GGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTG 6 0.15 No Hit GGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTT 5 0.125 No Hit GGGAGGGGCTTGGCTACAATTCCGAATACGCTATTACATGTTTGCGCTAG 5 0.125 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 5 0.125 No Hit GGGCAAGGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAA 5 0.125 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 5 0.125 No Hit GGGGTGTTGTAGGTCACCGAGGCTGTTCTGAGATTGCGACCAAGCACGTA 5 0.125 No Hit GGAGCACAACAAGGTAATTTGCCCGTCCCAGAAGGTTGCACTGACCGGAA 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GGAGAATAGGGTGCCGGCTAGTGACGAGGTTGGCACCAAAAGGATTCGCC 5 0.125 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 5 0.125 No Hit GGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123224 READS because READLEN < 1 Read 123224 spots for ERR6133447.sra Written 123224 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra Rejected 123217 READS because READLEN < 1 Read 123217 spots for ERR6133447.sra Written 123217 spots for ERR6133447.sra SRR ids: ['ERR6133447.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_v_2t69x4 ERR6133447.sra spots: 2464347 blocks: [[1, 123217], [123218, 246434], [246435, 369651], [369652, 492868], [492869, 616085], [616086, 739302], [739303, 862519], [862520, 985736], [985737, 1108953], [1108954, 1232170], [1232171, 1355387], [1355388, 1478604], [1478605, 1601821], [1601822, 1725038], [1725039, 1848255], [1848256, 1971472], [1971473, 2094689], [2094690, 2217906], [2217907, 2341123], [2341124, 2464347]] ERR6133447 file size 544537 ERR6133447 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133447 ERR6133447_1.fastq Input file: ERR6133447_1.fastq trimmed: ERR6133447-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:13:38 2024 >> started Sat Dec 7 06:13:40 2024 >> done (1.240s) 2464347 reads processed; of these: 1206 ( 0.05%) short reads filtered out after trimming by size control 65 ( 0.00%) empty reads filtered out after trimming by size control 2463076 (99.95%) reads available; of these: 34164 ( 1.39%) trimmed reads available after processing 2428912 (98.61%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 27 0.00% 19 31 0.00% 20 18 0.00% 21 18 0.00% 22 10 0.00% 23 10 0.00% 24 8 0.00% 25 3 0.00% 26 5 0.00% 27 11 0.00% 28 7 0.00% 29 82 0.00% 30 9 0.00% 31 10 0.00% 32 8 0.00% 33 3 0.00% 34 4 0.00% 35 9 0.00% 36 4 0.00% 37 8 0.00% 38 14 0.00% 39 25 0.00% 40 41 0.00% 41 20 0.00% 42 15 0.00% 43 14 0.00% 44 15 0.00% 45 13 0.00% 46 14 0.00% 47 10 0.00% 48 14 0.00% 49 20 0.00% 50 17 0.00% 51 29 0.00% 52 18 0.00% 53 19 0.00% 54 19 0.00% 55 15 0.00% 56 11 0.00% 57 17 0.00% 58 17 0.00% 59 21 0.00% 60 10 0.00% 61 13 0.00% 62 2 0.00% 63 2 0.00% 64 4 0.00% 65 1 0.00% 66 1 0.00% 67 14 0.00% 68 23 0.00% 69 45 0.00% 70 4879 0.20% 71 4079 0.17% 72 4192 0.17% 73 3934 0.16% 74 4207 0.17% 75 3794 0.15% 76 3506 0.14% 77 3796 0.15% 78 4158 0.17% 79 4742 0.19% 80 4268 0.17% 81 4310 0.17% 82 4745 0.19% 83 4816 0.20% 84 3913 0.16% 85 65 0.00% 86 141 0.01% 87 180 0.01% 88 358 0.01% 89 857 0.03% 90 1970 0.08% 91 5816 0.24% 92 23171 0.94% 93 2366381 96.07% 2463076 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=12.56 fanout-score-rank=11 prefix-density=0.84 prefix-fanout=5.0 sequence=GAAGAAGAAGAAACGCATGGTGCCCTGCTTCCGTCTGTCGGCTGCTTGCTTGGCAACGGCAGAGCAGAGCTTGGTGCAGTAAAACTACTGGTTATACTCTCTGTATGTAAAGTTAAAATTTTCACACACAGCTATGTGCTAAAGGA criterion=fanout-score sequence-density=0.02 sequence-density-rank=26 fanout-score=49.07 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=6.1 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCGGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGACGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTATAGGGAAAACATAATTTATGAGACAATCATACTGTGCAACTCTTGGCTCCATCGATTAATTAATACTCCTTGTTATTGCTTGCATGGTGG Started job on | Dec 07 06:13:52 Started mapping on | Dec 07 06:13:53 Finished on | Dec 07 06:13:56 Mapping speed, Million of reads per hour | 2955.69 Number of input reads | 2463076 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 1911388 Uniquely mapped reads % | 77.60% Average mapped length | 92.26 Number of splices: Total | 108374 Number of splices: Annotated (sjdb) | 89194 Number of splices: GT/AG | 104696 Number of splices: GC/AG | 2690 Number of splices: AT/AC | 35 Number of splices: Non-canonical | 953 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 1.86 Insertion rate per base | 0.02% Insertion average length | 2.10 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 512599 % of reads mapped to multiple loci | 20.81% Number of reads mapped to too many loci | 8146 % of reads mapped to too many loci | 0.33% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.23% % of reads unmapped: other | 0.02% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 39089 39089 39089 N_multimapping 512599 512599 512599 N_noFeature 102248 121090 1827740 N_ambiguous 71540 6732 199 UnstrandedReadsAssigned:1737600 PositiveStrandReadsAssigned:1783566 NegativeStrandReadsAssigned:83449 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133447 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133447-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,463,076 reads, 2,174,978 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 985 rounds 52973 ERR6133447.ke.tsv 35125 ERR6133447.se.tsv 88098 total ==> ERR6133447.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 70 31.3814 PNS24243 293 194 0 0 KQK14069 1603 1504 67 27.4003 KQK14071 474 375 0 0 ==> ERR6133447.se.tsv <== BRADI_1g14170v3 67 BRADI_1g53295v3 14 BRADI_1g59795v3 39 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 15 BRADI_1g74790v3 13 BRADI_1g09890v3 0 BRADI_1g77505v3 63 BRADI_1g48960v3 0 ERR6133447 completed mapping pipeline successfully