Starting /dee2/code/volunteer_pipeline.sh ERR6133449 current disk space = 1545579708416 free memory = 1603163824 ERR6133449 SRAfilesize 761188792e2d19e5cba378c6f2679ec2 ERR6133449.sra ERR6133449.sra file validated ERR6133449 is single end ERR6133449 is conventional basespace ERR6133449 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133449_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.84075 37.0 33.0 37.0 33.0 37.0 2 36.137 37.0 37.0 37.0 33.0 37.0 3 35.23475 37.0 33.0 37.0 33.0 37.0 4 34.822 37.0 33.0 37.0 33.0 37.0 5 34.7315 37.0 33.0 37.0 27.0 37.0 6 35.17075 37.0 37.0 37.0 33.0 37.0 7 36.57525 37.0 37.0 40.0 33.0 40.0 8 36.78325 37.0 37.0 40.0 33.0 40.0 9 36.94625 37.0 37.0 40.0 33.0 40.0 10-11 36.89725 37.0 37.0 40.0 33.0 40.0 12-13 36.785624999999996 37.0 37.0 40.0 33.0 40.0 14-15 36.782 37.0 37.0 40.0 33.0 40.0 16-17 36.5925 37.0 37.0 40.0 33.0 40.0 18-19 36.322875 37.0 37.0 40.0 33.0 40.0 20-21 36.080124999999995 37.0 33.0 40.0 33.0 40.0 22-23 35.865875 37.0 33.0 40.0 33.0 40.0 24-25 36.002125 37.0 35.0 40.0 33.0 40.0 26-27 36.068 37.0 33.0 40.0 33.0 40.0 28-29 35.996750000000006 37.0 33.0 40.0 33.0 40.0 30-31 36.005625 37.0 35.0 40.0 33.0 40.0 32-33 35.985875 37.0 33.0 40.0 33.0 40.0 34-35 36.009625 37.0 33.0 40.0 33.0 40.0 36-37 35.860125 37.0 33.0 40.0 33.0 40.0 38-39 35.88125 37.0 33.0 40.0 33.0 40.0 40-41 35.761875 37.0 33.0 40.0 33.0 40.0 42-43 35.671375 37.0 33.0 40.0 33.0 40.0 44-45 35.451375 37.0 33.0 37.0 30.0 40.0 46-47 35.357375000000005 37.0 33.0 37.0 33.0 40.0 48-49 35.438874999999996 37.0 33.0 37.0 33.0 40.0 50-51 35.254999999999995 37.0 33.0 37.0 33.0 40.0 52-53 34.956625 37.0 33.0 37.0 30.0 40.0 54-55 34.848625 37.0 33.0 37.0 33.0 38.5 56-57 34.653125 37.0 33.0 37.0 27.0 37.0 58-59 34.1895 37.0 33.0 37.0 27.0 37.0 60-61 34.204125000000005 37.0 33.0 37.0 27.0 37.0 62-63 34.08 37.0 33.0 37.0 27.0 37.0 64-65 34.082 37.0 33.0 37.0 27.0 37.0 66-67 33.786874999999995 37.0 33.0 37.0 27.0 37.0 68-69 32.9395 35.0 33.0 37.0 27.0 37.0 70-71 33.08285662318114 33.0 33.0 37.0 27.0 37.0 72-73 33.54302220949797 37.0 33.0 37.0 27.0 37.0 74-75 33.663406706573056 37.0 33.0 37.0 27.0 37.0 76-77 33.597297659532984 37.0 33.0 37.0 27.0 37.0 78-79 33.627060953167685 37.0 33.0 37.0 27.0 37.0 80-81 33.40806799105303 37.0 33.0 37.0 27.0 37.0 82-83 33.244148508480905 33.0 33.0 37.0 27.0 37.0 84-85 33.13684525431049 33.0 33.0 37.0 27.0 37.0 86-87 32.920645161290324 33.0 33.0 37.0 27.0 37.0 88-89 32.99651612903226 33.0 33.0 37.0 27.0 37.0 90-91 32.84941935483871 33.0 33.0 37.0 27.0 37.0 92-93 32.73767741935484 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 22.0 21 14.0 22 24.0 23 32.0 24 27.0 25 31.0 26 49.0 27 54.0 28 68.0 29 70.0 30 104.0 31 140.0 32 185.0 33 233.0 34 302.0 35 566.0 36 818.0 37 859.0 38 395.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 89.225 3.225 1.8499999999999999 5.7 2 74.375 15.825 6.1 3.6999999999999997 3 36.55 39.875 13.025 10.549999999999999 4 36.425000000000004 28.225 17.8 17.549999999999997 5 25.45 33.225 22.375 18.95 6 20.674999999999997 40.400000000000006 24.3 14.625 7 36.35 29.599999999999998 18.975 15.075 8 32.475 28.849999999999998 21.55 17.125 9 26.924999999999997 28.000000000000004 26.625 18.45 10-11 27.125 26.75 26.55 19.575 12-13 29.562500000000004 24.75 26.05 19.6375 14-15 24.0375 28.8375 27.8125 19.3125 16-17 24.637500000000003 30.25 25.587500000000002 19.525000000000002 18-19 23.96549568696087 26.190773846730842 26.490811351418923 23.35291911488936 20-21 24.252657911194497 25.92870544090056 27.12945590994372 22.689180737961227 22-23 26.950000000000003 22.95 27.375 22.725 24-25 27.500000000000004 23.0875 28.249999999999996 21.1625 26-27 25.637500000000003 26.0375 28.487499999999997 19.8375 28-29 27.4125 25.112499999999997 25.5375 21.9375 30-31 27.2625 24.4125 26.5 21.825 32-33 24.8 27.212500000000002 26.400000000000002 21.587500000000002 34-35 26.85 23.9125 27.0125 22.225 36-37 24.56557069633704 24.615576947118388 28.82860357544693 21.990248781097637 38-39 25.490686335791974 24.8906113264158 29.666208276034506 19.95249406175772 40-41 27.500000000000004 25.15 25.674999999999997 21.675 42-43 26.04127579737336 27.054409005628514 25.340838023764857 21.56347717323327 44-45 24.337500000000002 24.224999999999998 28.1 23.3375 46-47 24.7 22.925 28.0875 24.2875 48-49 25.2625 25.0375 29.975 19.725 50-51 26.3 25.825 28.1625 19.7125 52-53 25.85775106436263 27.635862759829706 25.7450538442274 20.761332331580267 54-55 24.33108277069267 29.83245811452863 26.03150787696924 19.80495123780945 56-57 26.3125 25.275 26.987499999999997 21.425 58-59 25.05 23.8125 27.575 23.5625 60-61 24.0125 23.799999999999997 29.212500000000002 22.975 62-63 23.150000000000002 26.737499999999997 30.575000000000003 19.537499999999998 64-65 24.825 25.5625 28.1625 21.45 66-67 25.3125 26.0 28.262500000000003 20.424999999999997 68-69 23.7375 26.5875 26.787499999999998 22.8875 70-71 26.30853994490358 24.092161282243925 27.748559979964938 21.850738792887554 72-73 26.051902242378432 24.313429075333836 27.223481985386748 22.411186696900984 74-75 23.086654016445287 27.69133459835547 27.147375079063885 22.074636306135357 76-77 23.130633489907325 25.872794210994034 29.173543227116923 21.82302907198172 78-79 24.497327564265717 23.568337999490964 29.880376686179687 22.05395775006363 80-81 24.214157935088167 25.42806031178124 30.34755941732686 20.010222335803732 82-83 24.656831302116743 23.15586914688903 29.685695958948045 22.50160359204618 84-85 24.42325041886841 23.41796623276195 29.617218713751768 22.541564634617863 86-87 22.374193548387098 26.025806451612905 32.96774193548387 18.63225806451613 88-89 23.04516129032258 28.761290322580646 29.767741935483873 18.425806451612903 90-91 25.961290322580645 25.49677419354839 28.63225806451613 19.90967741935484 92-93 23.29032258064516 29.470967741935482 27.90967741935484 19.329032258064515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 2.5 18 2.0 19 0.5 20 1.0 21 3.5 22 4.5 23 8.0 24 8.5 25 3.5 26 4.0 27 7.0 28 17.5 29 26.0 30 28.0 31 24.5 32 29.5 33 47.5 34 58.0 35 70.0 36 94.5 37 116.5 38 145.5 39 149.0 40 150.0 41 175.0 42 186.0 43 189.5 44 201.5 45 198.0 46 211.0 47 206.0 48 162.5 49 170.5 50 162.0 51 159.0 52 175.5 53 183.0 54 152.0 55 100.0 56 78.5 57 66.0 58 74.0 59 65.0 60 42.5 61 40.5 62 48.0 63 51.5 64 44.5 65 37.0 66 31.0 67 24.0 68 20.5 69 20.0 70 14.5 71 8.0 72 6.0 73 5.0 74 3.5 75 3.5 76 3.0 77 0.5 78 1.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0125 20-21 0.0625 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0125 38-39 0.0125 40-41 0.0 42-43 0.0625 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.17500000000000002 54-55 0.025 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 14.0 71 13.0 72 8.0 73 7.0 74 11.0 75 6.0 76 5.0 77 4.0 78 6.0 79 8.0 80 10.0 81 6.0 82 9.0 83 9.0 84 9.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3875.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.075 #Duplication Level Percentage of deduplicated Percentage of total 1 94.26107641986322 79.25 2 3.4195658638120725 5.75 3 1.070472792149866 2.7 4 0.23788284269997023 0.8 5 0.17841213202497772 0.75 6 0.17841213202497772 0.8999999999999999 7 0.17841213202497772 1.05 8 0.02973535533749628 0.2 9 0.02973535533749628 0.22499999999999998 >10 0.3865596193874517 6.9750000000000005 >50 0.02973535533749628 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 56 1.4000000000000001 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 36 0.8999999999999999 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 34 0.8500000000000001 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 33 0.8250000000000001 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 32 0.8 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 24 0.6 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 22 0.5499999999999999 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 21 0.525 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 18 0.44999999999999996 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 14 0.35000000000000003 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 13 0.325 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 12 0.3 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 10 0.25 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 10 0.25 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 9 0.22499999999999998 No Hit GGGGAAGAAGACCTCTTTCTGGGAGGCCGAAGCCACTTCGGCACCGGCAC 8 0.2 No Hit GGGATGGAGATGGCCGCGGAGAACGGCGGCTGCGGCTGCAGCACCTGCAA 7 0.17500000000000002 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 7 0.17500000000000002 No Hit GGAGTCCCATATATATATGTATAAGATGCCAGCCCGGTTTCGTCAACCAT 7 0.17500000000000002 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 7 0.17500000000000002 No Hit GGAGTAAACTAGCATAAATAATTTGTTTGATGCCAGTCATCAAGGTTGAG 7 0.17500000000000002 No Hit GGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTG 7 0.17500000000000002 No Hit GAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAGTTTTTCTTA 6 0.15 No Hit GGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTT 6 0.15 No Hit GGATTTGAAGAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAG 6 0.15 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 6 0.15 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 6 0.15 No Hit GGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTG 6 0.15 No Hit GGGAGGGGCTTGGCTACAATTCCGAATACGCTATTACATGTTTGCGCTAG 5 0.125 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 5 0.125 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 5 0.125 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 5 0.125 No Hit GGGCGTGGCGTTCATGAACAAGTGAAACCTTATGGCTGGATGGGTCATCG 5 0.125 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.05 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.0625 0.0 0.0 0.0 0.0 28-29 0.075 0.0 0.0 0.0 0.0 30-31 0.075 0.0 0.0 0.0 0.0 32-33 0.075 0.0 0.0 0.0 0.0 34-35 0.075 0.0 0.0 0.0 0.0 36-37 0.0875 0.0 0.0 0.0 0.0 38-39 0.1 0.0 0.0 0.0 0.0 40-41 0.1 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.1125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCTCG 25 0.0019998178 35.927273 78-79 GAAGGTA 25 0.0019998178 35.927273 86-87 GCGATCT 30 0.005549756 29.181433 72-73 GCGCGAT 30 0.005549756 29.181433 70-71 >>END_MODULE Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67682 READS because READLEN < 1 Read 67682 spots for ERR6133449.sra Written 67682 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra Rejected 67674 READS because READLEN < 1 Read 67674 spots for ERR6133449.sra Written 67674 spots for ERR6133449.sra SRR ids: ['ERR6133449.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ytbnud4r ERR6133449.sra spots: 1353488 blocks: [[1, 67674], [67675, 135348], [135349, 203022], [203023, 270696], [270697, 338370], [338371, 406044], [406045, 473718], [473719, 541392], [541393, 609066], [609067, 676740], [676741, 744414], [744415, 812088], [812089, 879762], [879763, 947436], [947437, 1015110], [1015111, 1082784], [1082785, 1150458], [1150459, 1218132], [1218133, 1285806], [1285807, 1353488]] ERR6133449 file size 297415 ERR6133449 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133449 ERR6133449_1.fastq Input file: ERR6133449_1.fastq trimmed: ERR6133449-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:16:04 2024 >> started Sat Dec 7 06:16:05 2024 >> done (0.819s) 1353488 reads processed; of these: 242 ( 0.02%) short reads filtered out after trimming by size control 16 ( 0.00%) empty reads filtered out after trimming by size control 1353230 (99.98%) reads available; of these: 23981 ( 1.77%) trimmed reads available after processing 1329249 (98.23%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 25 0.00% 19 45 0.00% 20 23 0.00% 21 19 0.00% 22 25 0.00% 23 12 0.00% 24 17 0.00% 25 8 0.00% 26 9 0.00% 27 17 0.00% 28 26 0.00% 29 23 0.00% 30 14 0.00% 31 24 0.00% 32 21 0.00% 33 18 0.00% 34 23 0.00% 35 47 0.00% 36 460 0.03% 37 21 0.00% 38 26 0.00% 39 54 0.00% 40 31 0.00% 41 39 0.00% 42 12 0.00% 43 15 0.00% 44 38 0.00% 45 18 0.00% 46 31 0.00% 47 14 0.00% 48 6 0.00% 49 3 0.00% 50 17 0.00% 51 68 0.01% 52 17 0.00% 53 3 0.00% 54 5 0.00% 55 8 0.00% 56 21 0.00% 57 32 0.00% 58 8 0.00% 59 5 0.00% 60 10 0.00% 61 7 0.00% 62 0 0.00% 63 1 0.00% 64 2 0.00% 65 1 0.00% 66 3 0.00% 67 4 0.00% 68 16 0.00% 69 53 0.00% 70 4375 0.32% 71 4225 0.31% 72 4013 0.30% 73 3955 0.29% 74 3956 0.29% 75 4128 0.31% 76 3499 0.26% 77 3303 0.24% 78 3360 0.25% 79 3441 0.25% 80 3224 0.24% 81 3087 0.23% 82 3866 0.29% 83 4013 0.30% 84 3210 0.24% 85 77 0.01% 86 110 0.01% 87 200 0.01% 88 332 0.02% 89 584 0.04% 90 1315 0.10% 91 3995 0.30% 92 15221 1.12% 93 1274296 94.17% 1353230 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=5.52 fanout-score-rank=18 prefix-density=0.87 prefix-fanout=2.4 sequence=GCCGCCGCCGCCAAGGAAGGCATGTTCGTCAAGAACTACAGCTACTGATCCTAATCGCATCAAGCTTCAACGCCTGTGAGTGAAAACCAGTGATGAGAGTGCTGCTGCTAGCTAGCGCCGGCATTGATGAGCTTGAGAGGGCACTGTAGCCAGTGTGTCAGTCGTTGTTAAATTACAGGTTGAGATCATCAGCGTACTCCGATGGGAGGTGGACATCAGAAAGTATACTGTGTTTTACCACCCTAATTAAGTAAACAACTTTTGGAATGGTGATCATCTTACTGTTTTAAATAAATCTGTTTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=147.80 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=7.8 sequence=AGGAAGAGGAGGATGCAGTCAGGGCTCACAAACAACCTGCCACTGCCGCCATTGGCCTTCTAAAACAGGAGAGGAGGGGTTAGATAGTTTCGATCTGCAAGGGGGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGCAGAAGCTGTATGCTTATGAGCAGCTATGGTACTTATCGAGGACAGTAGCGTACT Started job on | Dec 07 06:16:27 Started mapping on | Dec 07 06:16:27 Finished on | Dec 07 06:16:31 Mapping speed, Million of reads per hour | 1217.91 Number of input reads | 1353230 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 999787 Uniquely mapped reads % | 73.88% Average mapped length | 92.21 Number of splices: Total | 70786 Number of splices: Annotated (sjdb) | 59733 Number of splices: GT/AG | 69082 Number of splices: GC/AG | 1338 Number of splices: AT/AC | 28 Number of splices: Non-canonical | 338 Mismatch rate per base, % | 0.46% Deletion rate per base | 0.03% Deletion average length | 1.82 Insertion rate per base | 0.01% Insertion average length | 1.94 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 285108 % of reads mapped to multiple loci | 21.07% Number of reads mapped to too many loci | 5672 % of reads mapped to too many loci | 0.42% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.59% % of reads unmapped: other | 0.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 68335 68335 68335 N_multimapping 285108 285108 285108 N_noFeature 55653 64432 958004 N_ambiguous 36678 3697 104 UnstrandedReadsAssigned:907456 PositiveStrandReadsAssigned:931658 NegativeStrandReadsAssigned:41679 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133449 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133449-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,353,230 reads, 1,149,243 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 908 rounds 52973 ERR6133449.ke.tsv 35125 ERR6133449.se.tsv 88098 total ==> ERR6133449.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 35 29.1019 PNS24243 293 194 0 0 KQK14069 1603 1504 7 5.30956 KQK14071 474 375 0 0 ==> ERR6133449.se.tsv <== BRADI_1g14170v3 7 BRADI_1g53295v3 12 BRADI_1g59795v3 10 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 24 BRADI_1g74790v3 3 BRADI_1g09890v3 0 BRADI_1g77505v3 26 BRADI_1g48960v3 0 ERR6133449 completed mapping pipeline successfully