Starting /dee2/code/volunteer_pipeline.sh ERR6133450 current disk space = 1545829949440 free memory = 1435328252 ERR6133450 SRAfilesize 7dbbf0489f7ab37d40da8dbbbe5f0cdc ERR6133450.sra ERR6133450.sra file validated ERR6133450 is single end ERR6133450 is conventional basespace ERR6133450 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133450_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.8375 37.0 33.0 37.0 33.0 37.0 2 36.12025 37.0 37.0 37.0 33.0 37.0 3 35.341 37.0 33.0 37.0 33.0 37.0 4 34.941 37.0 37.0 37.0 33.0 37.0 5 34.73275 37.0 33.0 37.0 27.0 37.0 6 35.2585 37.0 37.0 37.0 33.0 37.0 7 36.6915 37.0 37.0 40.0 33.0 40.0 8 36.89725 37.0 37.0 40.0 33.0 40.0 9 36.9205 37.0 37.0 40.0 33.0 40.0 10-11 36.934 37.0 37.0 40.0 33.0 40.0 12-13 36.92 37.0 37.0 40.0 33.0 40.0 14-15 36.80575 37.0 37.0 40.0 33.0 40.0 16-17 36.568375 37.0 37.0 40.0 33.0 40.0 18-19 36.2335 37.0 37.0 40.0 33.0 40.0 20-21 36.182249999999996 37.0 35.0 40.0 33.0 40.0 22-23 35.841875 37.0 33.0 40.0 33.0 40.0 24-25 36.0615 37.0 35.0 40.0 33.0 40.0 26-27 36.117000000000004 37.0 35.0 40.0 33.0 40.0 28-29 36.060500000000005 37.0 37.0 40.0 33.0 40.0 30-31 36.048500000000004 37.0 33.0 40.0 33.0 40.0 32-33 36.04775 37.0 35.0 40.0 33.0 40.0 34-35 36.09525 37.0 33.0 40.0 33.0 40.0 36-37 36.10825 37.0 35.0 40.0 33.0 40.0 38-39 36.074875 37.0 33.0 40.0 33.0 40.0 40-41 35.83 37.0 33.0 40.0 33.0 40.0 42-43 35.852875 37.0 33.0 40.0 33.0 40.0 44-45 35.648250000000004 37.0 33.0 38.5 33.0 40.0 46-47 35.585875 37.0 33.0 37.0 33.0 40.0 48-49 35.572374999999994 37.0 33.0 37.0 33.0 40.0 50-51 35.351875 37.0 33.0 37.0 33.0 40.0 52-53 35.0215 37.0 33.0 37.0 27.0 40.0 54-55 35.02875 37.0 33.0 37.0 30.0 40.0 56-57 34.867625000000004 37.0 33.0 37.0 30.0 38.5 58-59 34.27675 37.0 33.0 37.0 27.0 37.0 60-61 34.403999999999996 37.0 33.0 37.0 27.0 37.0 62-63 34.349374999999995 37.0 33.0 37.0 27.0 37.0 64-65 34.233374999999995 37.0 33.0 37.0 27.0 37.0 66-67 33.924375 37.0 33.0 37.0 27.0 37.0 68-69 33.119875 35.0 33.0 37.0 27.0 37.0 70-71 33.280660597689604 35.0 33.0 37.0 27.0 37.0 72-73 33.58684125063532 37.0 33.0 37.0 27.0 37.0 74-75 33.766740298889374 37.0 33.0 37.0 27.0 37.0 76-77 33.78220127817502 37.0 33.0 37.0 27.0 37.0 78-79 33.59870868692894 37.0 33.0 37.0 27.0 37.0 80-81 33.5603979360865 37.0 33.0 37.0 27.0 37.0 82-83 33.21481614066519 35.0 33.0 37.0 27.0 37.0 84-85 33.07625657943244 33.0 33.0 37.0 27.0 37.0 86-87 33.03945249597423 33.0 33.0 37.0 27.0 37.0 88-89 33.2337627482555 33.0 33.0 37.0 27.0 37.0 90-91 32.94216317767042 33.0 33.0 37.0 27.0 37.0 92-93 32.85185185185185 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 11.0 21 20.0 22 14.0 23 20.0 24 31.0 25 34.0 26 36.0 27 62.0 28 63.0 29 93.0 30 100.0 31 158.0 32 187.0 33 237.0 34 271.0 35 518.0 36 783.0 37 851.0 38 487.0 39 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 83.15 7.6 2.85 6.4 2 68.7 18.6 8.025 4.675 3 37.95 36.625 13.600000000000001 11.825 4 34.725 28.4 18.099999999999998 18.775 5 25.5 31.85 25.025 17.625 6 20.349999999999998 39.550000000000004 25.1 15.0 7 33.5 31.5 19.950000000000003 15.049999999999999 8 30.55 28.025 24.025 17.4 9 25.575 28.225 28.375 17.825 10-11 25.7375 27.5125 27.787499999999998 18.9625 12-13 29.475 24.575 26.625 19.325 14-15 23.6375 29.049999999999997 28.725 18.587500000000002 16-17 25.1875 30.912499999999998 25.5125 18.387500000000003 18-19 24.265533191648956 27.365920740092513 25.603200400050007 22.765345668208525 20-21 24.78418616289253 26.160390341548855 27.27386463155261 21.781558864006005 22-23 26.325 24.7375 27.0875 21.85 24-25 27.8375 23.849999999999998 27.4125 20.9 26-27 26.6125 26.237500000000004 28.625 18.525 28-29 25.637500000000003 26.437500000000004 27.5125 20.4125 30-31 28.962500000000002 25.5375 24.95 20.549999999999997 32-33 24.6 28.787499999999998 26.0375 20.575 34-35 26.1 24.087500000000002 27.275 22.537499999999998 36-37 25.028128516064506 23.365420677584698 29.59119889986248 22.015251906488313 38-39 25.828228528566072 24.8906113264158 30.90386298287286 18.37729716214527 40-41 27.187499999999996 25.6125 26.5875 20.6125 42-43 25.41906429822367 29.1343507630723 25.66925193895422 19.777332999749813 44-45 24.5375 25.4625 27.800000000000004 22.2 46-47 25.75 22.475 26.6 25.174999999999997 48-49 25.275 24.775 29.8875 20.0625 50-51 26.1125 25.3125 28.5875 19.9875 52-53 24.141819092959157 28.639438737158606 26.246554748183414 20.972187421698823 54-55 23.452931616452055 29.303662957869737 26.878359794974372 20.365045630703836 56-57 26.2875 25.674999999999997 27.3625 20.674999999999997 58-59 23.2875 24.7375 29.049999999999997 22.925 60-61 25.587500000000002 24.4875 28.549999999999997 21.375 62-63 22.3375 27.875 30.7 19.0875 64-65 24.2875 28.199999999999996 27.212500000000002 20.3 66-67 25.35 26.950000000000003 27.5625 20.1375 68-69 23.97799724965621 26.16577072134017 27.21590198774847 22.640330041255158 70-71 26.785266850413432 24.56777749937359 27.261338010523676 21.3856176396893 72-73 25.92639433413431 23.207284684456813 28.468445681042116 22.397875300366763 74-75 23.811957077158915 27.784874808380174 27.7082268778743 20.69494123658661 76-77 22.589389441073966 26.95236865883568 29.056408932489997 21.401832967600363 78-79 24.58995053371518 24.30356677948451 29.588648789377764 21.51783389742255 80-81 25.269099501181415 26.76555526384878 29.02336571278551 18.941979522184297 82-83 25.04638218923933 23.124834349324143 29.896633978266628 21.93214948316989 84-85 25.107123727905734 23.99571505088377 28.950187466523836 21.946973754686663 86-87 22.705314009661837 26.610305958132045 31.40096618357488 19.28341384863124 88-89 22.41009125067096 29.09286097691895 29.696725711218463 18.800322061191626 90-91 26.03327965646806 27.12023617820719 28.44873859366613 18.397745571658618 92-93 23.201825013419217 29.965110037573805 27.938808373590984 18.894256575415998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.5 18 5.0 19 3.0 20 5.0 21 6.0 22 5.5 23 5.0 24 8.5 25 10.5 26 9.0 27 14.0 28 22.0 29 29.0 30 36.0 31 39.5 32 42.0 33 53.5 34 67.0 35 90.0 36 118.5 37 133.5 38 148.0 39 154.0 40 156.5 41 167.5 42 165.0 43 157.5 44 162.5 45 161.5 46 211.5 47 210.0 48 171.5 49 183.0 50 167.5 51 162.0 52 165.0 53 184.0 54 177.0 55 115.0 56 78.0 57 76.5 58 69.0 59 58.0 60 44.0 61 41.5 62 43.0 63 33.0 64 31.0 65 29.0 66 23.0 67 19.5 68 17.5 69 13.5 70 7.0 71 5.0 72 3.5 73 4.0 74 3.5 75 1.0 76 1.5 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0125 20-21 0.08750000000000001 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0125 38-39 0.0125 40-41 0.0 42-43 0.075 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.22499999999999998 54-55 0.0125 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0125 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 18.0 71 19.0 72 19.0 73 26.0 74 8.0 75 26.0 76 21.0 77 13.0 78 18.0 79 12.0 80 22.0 81 16.0 82 18.0 83 22.0 84 16.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3726.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.95 #Duplication Level Percentage of deduplicated Percentage of total 1 93.05126621371217 75.325 2 4.014823965410748 6.5 3 1.266213712168005 3.075 4 0.586781964175417 1.9 5 0.2779493514515133 1.125 6 0.21618282890673254 1.05 7 0.0926497838171711 0.525 8 0.030883261272390366 0.2 9 0.030883261272390366 0.22499999999999998 >10 0.3705991352686844 6.7250000000000005 >50 0.06176652254478073 3.35 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 71 1.775 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 63 1.575 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 44 1.0999999999999999 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 35 0.8750000000000001 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 34 0.8500000000000001 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 34 0.8500000000000001 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 25 0.625 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 22 0.5499999999999999 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 17 0.42500000000000004 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 14 0.35000000000000003 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 12 0.3 No Hit GGGCAAGGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAA 11 0.27499999999999997 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 11 0.27499999999999997 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 10 0.25 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 9 0.22499999999999998 No Hit GGAGCACAACAAGGTAATTTGCCCGTCCCAGAAGGTTGCACTGACCGGAA 8 0.2 No Hit GGGAGGGGCTTGGCTACAATTCCGAATACGCTATTACATGTTTGCGCTAG 7 0.17500000000000002 No Hit GGAGTCCCATATATATATGTATAAGATGCCAGCCCGGTTTCGTCAACCAT 7 0.17500000000000002 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 7 0.17500000000000002 No Hit GGGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACG 6 0.15 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 6 0.15 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 6 0.15 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 6 0.15 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 6 0.15 No Hit GGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTG 6 0.15 No Hit GGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTG 6 0.15 No Hit GGATTTGAAGAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAG 5 0.125 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 5 0.125 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 5 0.125 No Hit AGGTCAACCTTTTGAACTGCCTGCTGAATCCATGAGCAGGCAAGAGACAA 5 0.125 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 5 0.125 No Hit GGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACC 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 5 0.125 No Hit GGGCTTGGCTACAATTCCGAATACGCTATTACATGTTTGCGCTAGTTTTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.125 0.0 0.0 0.0 0.0 42-43 0.15 0.0 0.0 0.0 0.0 44-45 0.15 0.0 0.0 0.0 0.0 46-47 0.15 0.0 0.0 0.0 0.0 48-49 0.15 0.0 0.0 0.0 0.0 50-51 0.15 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.2 0.0 0.0 0.0 0.0 58-59 0.2 0.0 0.0 0.0 0.0 60-61 0.2 0.0 0.0 0.0 0.0 62-63 0.21250000000000002 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.225 0.0 0.0 0.0 0.0 70-71 0.225 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86645 READS because READLEN < 1 Read 86645 spots for ERR6133450.sra Written 86645 spots for ERR6133450.sra Rejected 86654 READS because READLEN < 1 Read 86654 spots for ERR6133450.sra Written 86654 spots for ERR6133450.sra SRR ids: ['ERR6133450.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_oqih3n2b ERR6133450.sra spots: 1732909 blocks: [[1, 86645], [86646, 173290], [173291, 259935], [259936, 346580], [346581, 433225], [433226, 519870], [519871, 606515], [606516, 693160], [693161, 779805], [779806, 866450], [866451, 953095], [953096, 1039740], [1039741, 1126385], [1126386, 1213030], [1213031, 1299675], [1299676, 1386320], [1386321, 1472965], [1472966, 1559610], [1559611, 1646255], [1646256, 1732909]] ERR6133450 file size 379387 ERR6133450 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133450 ERR6133450_1.fastq Input file: ERR6133450_1.fastq trimmed: ERR6133450-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:18:12 2024 >> started Sat Dec 7 06:18:13 2024 >> done (1.354s) 1732909 reads processed; of these: 508 ( 0.03%) short reads filtered out after trimming by size control 14 ( 0.00%) empty reads filtered out after trimming by size control 1732387 (99.97%) reads available; of these: 31626 ( 1.83%) trimmed reads available after processing 1700761 (98.17%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 54 0.00% 19 197 0.01% 20 46 0.00% 21 64 0.00% 22 37 0.00% 23 40 0.00% 24 22 0.00% 25 25 0.00% 26 19 0.00% 27 38 0.00% 28 49 0.00% 29 52 0.00% 30 23 0.00% 31 27 0.00% 32 42 0.00% 33 27 0.00% 34 32 0.00% 35 86 0.00% 36 476 0.03% 37 40 0.00% 38 78 0.00% 39 211 0.01% 40 114 0.01% 41 122 0.01% 42 31 0.00% 43 55 0.00% 44 185 0.01% 45 106 0.01% 46 114 0.01% 47 40 0.00% 48 47 0.00% 49 13 0.00% 50 75 0.00% 51 377 0.02% 52 68 0.00% 53 15 0.00% 54 21 0.00% 55 12 0.00% 56 48 0.00% 57 234 0.01% 58 44 0.00% 59 37 0.00% 60 53 0.00% 61 22 0.00% 62 0 0.00% 63 2 0.00% 64 1 0.00% 65 6 0.00% 66 5 0.00% 67 11 0.00% 68 29 0.00% 69 103 0.01% 70 10956 0.63% 71 10391 0.60% 72 9248 0.53% 73 9396 0.54% 74 9255 0.53% 75 9511 0.55% 76 7962 0.46% 77 7790 0.45% 78 8880 0.51% 79 8159 0.47% 80 7972 0.46% 81 8049 0.46% 82 8727 0.50% 83 10840 0.63% 84 7368 0.43% 85 77 0.00% 86 122 0.01% 87 212 0.01% 88 393 0.02% 89 752 0.04% 90 1702 0.10% 91 4930 0.28% 92 18333 1.06% 93 1567687 90.49% 1732387 reads passed initial QC criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=1.80 fanout-score-rank=30 prefix-density=0.94 prefix-fanout=1.0 sequence=ATTCGAGTTCGCGCCGGTAGATACTATCGATTAATAGATAAAACTAAATATGAAGAAGGTCTAAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=71.92 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=6.3 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTAT Started job on | Dec 07 06:18:33 Started mapping on | Dec 07 06:18:33 Finished on | Dec 07 06:18:39 Mapping speed, Million of reads per hour | 1039.43 Number of input reads | 1732387 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 1076629 Uniquely mapped reads % | 62.15% Average mapped length | 92.18 Number of splices: Total | 68935 Number of splices: Annotated (sjdb) | 56098 Number of splices: GT/AG | 66530 Number of splices: GC/AG | 1663 Number of splices: AT/AC | 44 Number of splices: Non-canonical | 698 Mismatch rate per base, % | 0.48% Deletion rate per base | 0.03% Deletion average length | 1.80 Insertion rate per base | 0.03% Insertion average length | 2.27 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 446614 % of reads mapped to multiple loci | 25.78% Number of reads mapped to too many loci | 13751 % of reads mapped to too many loci | 0.79% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.18% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 209144 209144 209144 N_multimapping 446614 446614 446614 N_noFeature 62225 71683 1032279 N_ambiguous 39046 4140 153 UnstrandedReadsAssigned:975358 PositiveStrandReadsAssigned:1000806 NegativeStrandReadsAssigned:44197 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133450 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133450-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,732,387 reads, 1,328,446 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 878 rounds 52973 ERR6133450.ke.tsv 35125 ERR6133450.se.tsv 88098 total ==> ERR6133450.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 33 23.4293 PNS24243 293 194 0 0 KQK14069 1603 1504 25 16.1916 KQK14071 474 375 0 0 ==> ERR6133450.se.tsv <== BRADI_1g14170v3 25 BRADI_1g53295v3 8 BRADI_1g59795v3 14 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 11 BRADI_1g74790v3 9 BRADI_1g09890v3 0 BRADI_1g77505v3 20 BRADI_1g48960v3 0 ERR6133450 completed mapping pipeline successfully