Starting /dee2/code/volunteer_pipeline.sh ERR6133452 current disk space = 1545719259136 free memory = 1449112332 ERR6133452 SRAfilesize 9e5382db09c30172e4c7c0a6cd752d4f ERR6133452.sra ERR6133452.sra file validated ERR6133452 is single end ERR6133452 is conventional basespace ERR6133452 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133452_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.73825 37.0 33.0 37.0 33.0 37.0 2 36.13525 37.0 37.0 37.0 33.0 37.0 3 35.21675 37.0 33.0 37.0 33.0 37.0 4 34.837 37.0 33.0 37.0 33.0 37.0 5 34.6955 37.0 33.0 37.0 27.0 37.0 6 35.154 37.0 37.0 37.0 33.0 37.0 7 36.6965 37.0 37.0 40.0 33.0 40.0 8 36.823 37.0 37.0 40.0 33.0 40.0 9 36.91925 37.0 37.0 40.0 33.0 40.0 10-11 36.9345 37.0 37.0 40.0 33.0 40.0 12-13 36.81375 37.0 37.0 40.0 33.0 40.0 14-15 36.715999999999994 37.0 37.0 40.0 33.0 40.0 16-17 36.508375 37.0 37.0 40.0 33.0 40.0 18-19 36.08175 37.0 35.0 40.0 33.0 40.0 20-21 36.05975 37.0 33.0 40.0 33.0 40.0 22-23 35.660875000000004 37.0 33.0 40.0 30.0 40.0 24-25 35.844375 37.0 33.0 40.0 33.0 40.0 26-27 36.018625 37.0 35.0 40.0 33.0 40.0 28-29 35.94825 37.0 33.0 40.0 33.0 40.0 30-31 36.08 37.0 35.0 40.0 33.0 40.0 32-33 35.945125 37.0 33.0 40.0 33.0 40.0 34-35 35.933 37.0 33.0 40.0 33.0 40.0 36-37 36.01575 37.0 33.0 40.0 33.0 40.0 38-39 35.8945 37.0 33.0 40.0 33.0 40.0 40-41 35.75875 37.0 33.0 40.0 33.0 40.0 42-43 35.72225 37.0 33.0 40.0 33.0 40.0 44-45 35.521249999999995 37.0 33.0 38.5 33.0 40.0 46-47 35.44375 37.0 33.0 37.0 33.0 40.0 48-49 35.482 37.0 33.0 37.0 33.0 40.0 50-51 35.268874999999994 37.0 33.0 37.0 33.0 40.0 52-53 35.001 37.0 33.0 37.0 30.0 40.0 54-55 34.894375 37.0 33.0 37.0 30.0 38.5 56-57 34.753125 37.0 33.0 37.0 30.0 37.0 58-59 34.24325 37.0 33.0 37.0 27.0 37.0 60-61 34.29125 37.0 33.0 37.0 27.0 37.0 62-63 34.251000000000005 37.0 33.0 37.0 27.0 37.0 64-65 34.209625 37.0 33.0 37.0 27.0 37.0 66-67 33.98725 37.0 33.0 37.0 27.0 37.0 68-69 33.147875 35.0 33.0 37.0 27.0 37.0 70-71 33.36768086172344 35.0 33.0 37.0 27.0 37.0 72-73 33.709791290024256 37.0 33.0 37.0 27.0 37.0 74-75 33.82540790636676 37.0 33.0 37.0 27.0 37.0 76-77 33.8080858373477 37.0 33.0 37.0 27.0 37.0 78-79 33.793149704725394 37.0 33.0 37.0 27.0 37.0 80-81 33.713169478524335 37.0 33.0 37.0 27.0 37.0 82-83 33.51885920855436 37.0 33.0 37.0 27.0 37.0 84-85 33.35307914500211 35.0 33.0 37.0 27.0 37.0 86-87 33.18852034205753 33.0 33.0 37.0 27.0 37.0 88-89 33.26898160145116 37.0 33.0 37.0 27.0 37.0 90-91 32.93443897382742 33.0 33.0 37.0 27.0 37.0 92-93 33.00233221041721 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 10.0 21 21.0 22 22.0 23 24.0 24 29.0 25 38.0 26 41.0 27 62.0 28 96.0 29 96.0 30 86.0 31 125.0 32 157.0 33 201.0 34 310.0 35 523.0 36 844.0 37 857.0 38 448.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 88.14999999999999 2.9499999999999997 2.7 6.2 2 72.1 16.7 6.7 4.5 3 38.074999999999996 37.7 13.4 10.825 4 34.949999999999996 28.225 18.575 18.25 5 24.25 31.724999999999998 23.200000000000003 20.825 6 20.5 37.95 25.5 16.05 7 35.699999999999996 28.425 20.925 14.95 8 32.125 30.15 22.95 14.774999999999999 9 28.775000000000002 26.875 26.900000000000002 17.45 10-11 27.425 26.8 27.0 18.775 12-13 29.575000000000003 25.025 28.237499999999997 17.1625 14-15 23.1625 27.4125 29.6375 19.787499999999998 16-17 25.0125 30.1375 26.787499999999998 18.0625 18-19 24.3125 26.625 27.525 21.5375 20-21 24.60615153788447 25.756439109777446 27.894473618404604 21.742935733933482 22-23 27.425 24.2625 26.924999999999997 21.3875 24-25 25.575 24.875 28.175 21.375 26-27 24.625 26.025 29.375 19.975 28-29 25.2625 26.0 28.262500000000003 20.474999999999998 30-31 26.137500000000003 24.825 28.599999999999998 20.4375 32-33 24.5375 26.6 28.4125 20.45 34-35 26.187500000000004 24.675 28.1 21.0375 36-37 24.775 24.9125 28.6625 21.65 38-39 25.406351587896975 24.568642160540136 29.994998749687422 20.030007501875467 40-41 26.540817602200274 24.82810351293912 27.82847855981998 20.80260032504063 42-43 24.471147828263863 26.924521216672925 28.02603579922393 20.578295155839278 44-45 24.081020255063766 25.78144536134033 28.59464866216554 21.54288572143036 46-47 24.7875 24.675 28.6375 21.9 48-49 24.4 25.087500000000002 30.2375 20.275000000000002 50-51 25.05 26.075 29.562500000000004 19.3125 52-53 23.838737949167395 26.042318767997997 29.122323776136223 20.996619506698387 54-55 23.627953494186773 27.453431678959873 28.403550443805475 20.515064383047882 56-57 24.712500000000002 24.6625 30.725 19.900000000000002 58-59 24.6875 24.837500000000002 29.45 21.025 60-61 24.725 25.2125 28.9375 21.125 62-63 23.1625 27.6 31.3 17.9375 64-65 23.8375 25.387500000000003 30.525000000000002 20.25 66-67 24.2625 26.775 29.849999999999998 19.112499999999997 68-69 22.39029878734842 26.02825353169146 31.203900487560944 20.377547193399177 70-71 25.3003003003003 24.54954954954955 30.055055055055057 20.095095095095093 72-73 25.364688128772634 25.32696177062374 29.036720321931593 20.271629778672033 74-75 22.862195275988377 26.41151951496779 30.64292029809271 20.08336491095112 76-77 23.681870156269852 25.48596112311015 29.729386354973954 21.102782365646043 78-79 23.710418794688458 24.43820224719101 32.35444330949949 19.49693564862104 80-81 23.71252882398155 25.736612861901104 31.040225467589032 19.51063284652831 82-83 24.199974296362935 24.94537977123763 30.947179025832156 19.90746690656728 84-85 23.36351875808538 25.510996119016816 31.423027166882278 19.70245795601552 86-87 23.075926405804612 25.14900233221042 32.68981601451153 19.08525524747344 88-89 22.45400362788287 26.522415133454263 31.212749416947393 19.81083182171547 90-91 25.537704068411504 25.783881834672194 29.21741383778181 19.461000259134494 92-93 22.90748898678414 27.908784659238144 30.46125939362529 18.722466960352424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.0 17 3.0 18 4.5 19 2.0 20 2.0 21 2.0 22 2.0 23 3.0 24 3.0 25 3.5 26 6.5 27 11.0 28 15.0 29 16.0 30 19.5 31 28.0 32 39.5 33 60.5 34 81.0 35 96.0 36 111.0 37 143.0 38 175.5 39 193.0 40 200.0 41 189.0 42 208.5 43 236.0 44 217.0 45 196.0 46 202.0 47 203.0 48 179.5 49 170.0 50 166.5 51 147.5 52 132.5 53 138.5 54 131.5 55 89.5 56 69.5 57 73.0 58 60.0 59 44.5 60 39.0 61 35.0 62 33.5 63 31.5 64 23.5 65 18.5 66 20.5 67 16.0 68 15.0 69 12.0 70 5.0 71 4.0 72 6.5 73 7.5 74 5.0 75 3.5 76 1.5 77 0.5 78 0.5 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.025 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.025 40-41 0.0125 42-43 0.13749999999999998 44-45 0.025 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.1625 54-55 0.0125 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0125 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 8.0 71 10.0 72 12.0 73 3.0 74 17.0 75 9.0 76 11.0 77 11.0 78 6.0 79 8.0 80 4.0 81 4.0 82 13.0 83 13.0 84 12.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3859.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.075 #Duplication Level Percentage of deduplicated Percentage of total 1 94.26625035481125 83.025 2 3.6048822026681804 6.35 3 1.0786261708770934 2.85 4 0.2838489923360772 1.0 5 0.22707919386886177 1.0 6 0.05676979846721544 0.3 7 0.11353959693443089 0.7000000000000001 8 0.11353959693443089 0.8 9 0.02838489923360772 0.22499999999999998 >10 0.22707919386886177 3.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 34 0.8500000000000001 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 21 0.525 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 19 0.475 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 18 0.44999999999999996 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 16 0.4 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 16 0.4 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 14 0.35000000000000003 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 12 0.3 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 9 0.22499999999999998 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 8 0.2 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 8 0.2 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 8 0.2 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 8 0.2 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 7 0.17500000000000002 No Hit GGGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACG 7 0.17500000000000002 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 7 0.17500000000000002 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 7 0.17500000000000002 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 6 0.15 No Hit ATCTAGGGGTAAAGCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAA 6 0.15 No Hit GCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAA 5 0.125 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 5 0.125 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 5 0.125 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 5 0.125 No Hit GGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAG 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 5 0.125 No Hit GGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0125 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.0625 0.0 0.0 0.0 0.0 38-39 0.1 0.0 0.0 0.0 0.0 40-41 0.1 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAAAA 25 0.0021308267 35.471794 86-87 >>END_MODULE Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93875 READS because READLEN < 1 Read 93875 spots for ERR6133452.sra Written 93875 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra Rejected 93866 READS because READLEN < 1 Read 93866 spots for ERR6133452.sra Written 93866 spots for ERR6133452.sra SRR ids: ['ERR6133452.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ru80jjfw ERR6133452.sra spots: 1877329 blocks: [[1, 93866], [93867, 187732], [187733, 281598], [281599, 375464], [375465, 469330], [469331, 563196], [563197, 657062], [657063, 750928], [750929, 844794], [844795, 938660], [938661, 1032526], [1032527, 1126392], [1126393, 1220258], [1220259, 1314124], [1314125, 1407990], [1407991, 1501856], [1501857, 1595722], [1595723, 1689588], [1689589, 1783454], [1783455, 1877329]] ERR6133452 file size 413534 ERR6133452 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133452 ERR6133452_1.fastq Input file: ERR6133452_1.fastq trimmed: ERR6133452-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:20:59 2024 >> started Sat Dec 7 06:21:01 2024 >> done (1.287s) 1877329 reads processed; of these: 161 ( 0.01%) short reads filtered out after trimming by size control 16 ( 0.00%) empty reads filtered out after trimming by size control 1877152 (99.99%) reads available; of these: 30887 ( 1.65%) trimmed reads available after processing 1846265 (98.35%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 34 0.00% 20 11 0.00% 21 10 0.00% 22 14 0.00% 23 9 0.00% 24 10 0.00% 25 7 0.00% 26 9 0.00% 27 19 0.00% 28 106 0.01% 29 32 0.00% 30 28 0.00% 31 22 0.00% 32 33 0.00% 33 41 0.00% 34 29 0.00% 35 31 0.00% 36 568 0.03% 37 23 0.00% 38 18 0.00% 39 42 0.00% 40 33 0.00% 41 40 0.00% 42 18 0.00% 43 17 0.00% 44 14 0.00% 45 15 0.00% 46 21 0.00% 47 10 0.00% 48 5 0.00% 49 3 0.00% 50 18 0.00% 51 36 0.00% 52 5 0.00% 53 4 0.00% 54 6 0.00% 55 7 0.00% 56 13 0.00% 57 8 0.00% 58 7 0.00% 59 6 0.00% 60 6 0.00% 61 10 0.00% 62 2 0.00% 63 5 0.00% 64 2 0.00% 65 3 0.00% 66 1 0.00% 67 13 0.00% 68 15 0.00% 69 64 0.00% 70 5261 0.28% 71 5048 0.27% 72 5271 0.28% 73 4734 0.25% 74 4879 0.26% 75 5067 0.27% 76 4623 0.25% 77 4485 0.24% 78 4678 0.25% 79 4890 0.26% 80 4304 0.23% 81 4300 0.23% 82 5458 0.29% 83 5615 0.30% 84 4603 0.25% 85 76 0.00% 86 145 0.01% 87 249 0.01% 88 415 0.02% 89 791 0.04% 90 1735 0.09% 91 5045 0.27% 92 19897 1.06% 93 1774064 94.51% 1877152 reads passed initial QC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=5.57 fanout-score-rank=21 prefix-density=0.70 prefix-fanout=3.9 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=26 fanout-score=311.71 fanout-score-rank=1 prefix-density=0.68 prefix-fanout=7.7 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTTCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAA Started job on | Dec 07 06:21:19 Started mapping on | Dec 07 06:21:19 Finished on | Dec 07 06:21:24 Mapping speed, Million of reads per hour | 1351.55 Number of input reads | 1877152 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 1496730 Uniquely mapped reads % | 79.73% Average mapped length | 92.07 Number of splices: Total | 63325 Number of splices: Annotated (sjdb) | 51227 Number of splices: GT/AG | 60306 Number of splices: GC/AG | 1775 Number of splices: AT/AC | 27 Number of splices: Non-canonical | 1217 Mismatch rate per base, % | 0.49% Deletion rate per base | 0.04% Deletion average length | 1.76 Insertion rate per base | 0.03% Insertion average length | 1.78 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 309739 % of reads mapped to multiple loci | 16.50% Number of reads mapped to too many loci | 10776 % of reads mapped to too many loci | 0.57% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.13% % of reads unmapped: other | 0.06% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 70683 70683 70683 N_multimapping 309739 309739 309739 N_noFeature 83612 97969 1429196 N_ambiguous 58736 5563 168 UnstrandedReadsAssigned:1354382 PositiveStrandReadsAssigned:1393198 NegativeStrandReadsAssigned:67366 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133452 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133452-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,877,152 reads, 1,604,185 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 976 rounds 52973 ERR6133452.ke.tsv 35125 ERR6133452.se.tsv 88098 total ==> ERR6133452.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 61 37.1805 PNS24243 293 194 0 0 KQK14069 1603 1504 30 16.6807 KQK14071 474 375 0 0 ==> ERR6133452.se.tsv <== BRADI_1g14170v3 30 BRADI_1g53295v3 26 BRADI_1g59795v3 8 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 29 BRADI_1g74790v3 12 BRADI_1g09890v3 0 BRADI_1g77505v3 47 BRADI_1g48960v3 0 ERR6133452 completed mapping pipeline successfully