Starting /dee2/code/volunteer_pipeline.sh ERR6133453 current disk space = 1545689571328 free memory = 1465606108 ERR6133453 SRAfilesize c14decd6ab24b0389b67c5d18c7c41f2 ERR6133453.sra ERR6133453.sra file validated ERR6133453 is single end ERR6133453 is conventional basespace ERR6133453 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133453_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.0095 37.0 33.0 37.0 33.0 37.0 2 36.048 37.0 37.0 37.0 33.0 37.0 3 35.24025 37.0 33.0 37.0 33.0 37.0 4 34.90525 37.0 33.0 37.0 33.0 37.0 5 34.73325 37.0 33.0 37.0 27.0 37.0 6 35.28475 37.0 37.0 37.0 33.0 37.0 7 36.77425 37.0 37.0 40.0 33.0 40.0 8 37.028 37.0 37.0 40.0 33.0 40.0 9 37.0615 37.0 37.0 40.0 33.0 40.0 10-11 36.89825 37.0 37.0 40.0 33.0 40.0 12-13 36.891000000000005 37.0 37.0 40.0 33.0 40.0 14-15 36.802625 37.0 37.0 40.0 33.0 40.0 16-17 36.615875 37.0 37.0 40.0 33.0 40.0 18-19 36.331625 37.0 37.0 40.0 33.0 40.0 20-21 36.2645 37.0 37.0 40.0 33.0 40.0 22-23 35.908625 37.0 33.0 40.0 33.0 40.0 24-25 36.067750000000004 37.0 35.0 40.0 33.0 40.0 26-27 36.254000000000005 37.0 37.0 40.0 33.0 40.0 28-29 36.10925 37.0 35.0 40.0 33.0 40.0 30-31 36.13275 37.0 37.0 40.0 33.0 40.0 32-33 36.145625 37.0 37.0 40.0 33.0 40.0 34-35 36.24275 37.0 37.0 40.0 33.0 40.0 36-37 36.21925 37.0 37.0 40.0 33.0 40.0 38-39 36.11125 37.0 37.0 40.0 33.0 40.0 40-41 36.011 37.0 33.0 40.0 33.0 40.0 42-43 36.04675 37.0 35.0 40.0 33.0 40.0 44-45 35.774375 37.0 33.0 40.0 33.0 40.0 46-47 35.695 37.0 33.0 37.0 33.0 40.0 48-49 35.742 37.0 33.0 37.0 33.0 40.0 50-51 35.531000000000006 37.0 33.0 37.0 33.0 40.0 52-53 35.26175 37.0 33.0 37.0 33.0 40.0 54-55 35.21275 37.0 33.0 37.0 33.0 40.0 56-57 35.068875 37.0 33.0 37.0 33.0 40.0 58-59 34.389 37.0 33.0 37.0 27.0 37.0 60-61 34.538875000000004 37.0 33.0 37.0 27.0 37.0 62-63 34.451875 37.0 33.0 37.0 27.0 37.0 64-65 34.32525 37.0 33.0 37.0 27.0 37.0 66-67 34.203875 37.0 33.0 37.0 27.0 37.0 68-69 33.330375000000004 35.0 33.0 37.0 27.0 37.0 70-71 33.46850251004016 35.0 33.0 37.0 27.0 37.0 72-73 33.8415930399711 37.0 33.0 37.0 27.0 37.0 74-75 33.90249344094224 37.0 33.0 37.0 27.0 37.0 76-77 33.95666135666136 37.0 33.0 37.0 27.0 37.0 78-79 33.849480263533465 37.0 33.0 37.0 27.0 37.0 80-81 33.76430464326371 37.0 33.0 37.0 27.0 37.0 82-83 33.64072549124862 37.0 33.0 37.0 27.0 37.0 84-85 33.500164480947404 35.0 33.0 37.0 27.0 37.0 86-87 33.195803261160115 33.0 33.0 37.0 27.0 37.0 88-89 33.41285752472601 37.0 33.0 37.0 27.0 37.0 90-91 33.213579256883186 33.0 33.0 37.0 27.0 37.0 92-93 33.201015771184174 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 9.0 21 10.0 22 20.0 23 21.0 24 28.0 25 34.0 26 41.0 27 54.0 28 71.0 29 72.0 30 111.0 31 142.0 32 158.0 33 187.0 34 297.0 35 506.0 36 821.0 37 899.0 38 488.0 39 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 85.95 4.7 2.8000000000000003 6.550000000000001 2 68.55 17.575 9.075 4.8 3 36.9 37.175000000000004 14.575 11.35 4 33.175 28.299999999999997 20.775 17.75 5 24.099999999999998 30.599999999999998 25.874999999999996 19.425 6 19.5 37.625 26.575 16.3 7 35.175 30.15 21.025 13.65 8 30.349999999999998 32.175 22.825 14.649999999999999 9 27.575 26.6 28.575 17.25 10-11 25.5625 27.700000000000003 27.950000000000003 18.787499999999998 12-13 28.675 25.6125 28.8625 16.85 14-15 22.325 28.0875 29.862499999999997 19.725 16-17 24.4 30.562499999999996 27.175 17.8625 18-19 24.25 26.2125 28.825 20.7125 20-21 24.455841881411057 24.78108581436077 29.347010257693267 21.4160620465349 22-23 25.2 24.2875 28.875 21.637500000000003 24-25 24.975 24.625 29.675 20.724999999999998 26-27 25.2125 24.8 30.562499999999996 19.425 28-29 25.15 25.7625 28.975 20.1125 30-31 26.375 24.224999999999998 29.312500000000004 20.0875 32-33 25.0625 25.900000000000002 28.787499999999998 20.25 34-35 25.2375 24.337500000000002 30.337500000000002 20.0875 36-37 24.375 24.6625 30.1375 20.825 38-39 25.0625 25.112499999999997 30.6375 19.1875 40-41 24.9375 25.2 29.037499999999998 20.825 42-43 24.25569176882662 27.107830873154864 29.20940705529147 19.427070302727046 44-45 23.5375 25.6125 30.837500000000002 20.0125 46-47 23.962500000000002 24.55 29.349999999999998 22.1375 48-49 23.3125 26.337500000000002 31.324999999999996 19.025 50-51 24.5125 26.087500000000002 29.9875 19.412499999999998 52-53 23.13573129464845 26.60734427873167 29.853365083343775 20.4035593432761 54-55 23.40585146286572 27.106776694173547 29.80745186296574 19.679919979995 56-57 25.387500000000003 25.5625 29.7875 19.2625 58-59 23.8125 25.324999999999996 30.9 19.9625 60-61 23.5125 24.7875 31.900000000000002 19.8 62-63 22.15 26.387500000000003 32.9875 18.475 64-65 23.200000000000003 26.075 31.724999999999998 19.0 66-67 22.875 25.7 31.7 19.725 68-69 22.925 26.8125 30.425 19.8375 70-71 24.774549098196395 24.611723446893787 31.563126252505008 19.05060120240481 72-73 23.791445203745887 25.57580359402683 30.52391799544419 20.108833206783093 74-75 23.57224990417785 25.143733231123033 30.765299603935098 20.51871726076402 76-77 22.97960237541957 25.484120836560802 31.655047766589206 19.881229021430418 78-79 22.900663976044786 25.113917458664236 31.896888425986198 20.088530139304776 80-81 22.31838447416732 26.304746918436926 32.86126409651193 18.51560451088382 82-83 22.538003965631194 24.600132187706546 31.61929940515532 21.24256444150694 84-85 23.666666666666668 24.253333333333334 32.266666666666666 19.813333333333333 86-87 22.60090884790163 25.421010425020047 33.42689120556001 18.55118952151831 88-89 21.69206094627105 27.84014969259556 31.60919540229885 18.858593958834536 90-91 23.336006415396955 26.396685378241113 31.76958032611601 18.49772788024592 92-93 22.039561614541565 28.81582464581663 31.061213579256886 18.083400160384926 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 3.0 18 3.0 19 0.5 20 0.0 21 0.5 22 2.0 23 5.0 24 6.0 25 5.0 26 6.0 27 13.0 28 22.5 29 26.0 30 32.0 31 42.5 32 60.5 33 75.0 34 76.5 35 101.0 36 135.5 37 171.0 38 208.5 39 229.0 40 228.0 41 212.5 42 217.5 43 229.0 44 218.5 45 194.5 46 190.5 47 182.0 48 171.5 49 169.5 50 153.0 51 137.5 52 122.5 53 128.5 54 117.5 55 76.5 56 56.0 57 52.5 58 51.0 59 42.0 60 30.5 61 26.0 62 23.5 63 20.0 64 16.5 65 15.0 66 11.5 67 6.0 68 6.5 69 6.0 70 7.0 71 11.0 72 8.0 73 3.5 74 1.0 75 0.0 76 1.5 77 1.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.075 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.075 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.2625 54-55 0.025 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 16.0 71 22.0 72 22.0 73 22.0 74 9.0 75 24.0 76 24.0 77 14.0 78 13.0 79 15.0 80 12.0 81 15.0 82 19.0 83 14.0 84 18.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3741.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.275 #Duplication Level Percentage of deduplicated Percentage of total 1 94.37132455894708 84.25 2 3.8364603752450295 6.8500000000000005 3 0.8120974516942034 2.175 4 0.33604032483898066 1.2 5 0.11201344161299356 0.5 6 0.16802016241949033 0.8999999999999999 7 0.02800336040324839 0.17500000000000002 8 0.02800336040324839 0.2 9 0.08401008120974517 0.675 >10 0.22402688322598713 3.075 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 33 0.8250000000000001 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 23 0.575 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 13 0.325 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 12 0.3 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 11 0.27499999999999997 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 11 0.27499999999999997 No Hit GACATCTAGGGGTAAAGCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACC 10 0.25 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 10 0.25 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 9 0.22499999999999998 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 9 0.22499999999999998 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 9 0.22499999999999998 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 8 0.2 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 7 0.17500000000000002 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 6 0.15 No Hit GGGAGGGGCTTGGCTACAATTCCGAATACGCTATTACATGTTTGCGCTAG 6 0.15 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 6 0.15 No Hit GGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTT 6 0.15 No Hit ATCTAGGGGTAAAGCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAA 6 0.15 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 6 0.15 No Hit GGGACAGTGATGGATTTCTTCATAAGGACGATGCGAGAGGCATATATGAT 5 0.125 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0125 0.0 0.0 0.0 0.0 36-37 0.037500000000000006 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83373 READS because READLEN < 1 Read 83373 spots for ERR6133453.sra Written 83373 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra Rejected 83364 READS because READLEN < 1 Read 83364 spots for ERR6133453.sra Written 83364 spots for ERR6133453.sra SRR ids: ['ERR6133453.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_99isruez ERR6133453.sra spots: 1667289 blocks: [[1, 83364], [83365, 166728], [166729, 250092], [250093, 333456], [333457, 416820], [416821, 500184], [500185, 583548], [583549, 666912], [666913, 750276], [750277, 833640], [833641, 917004], [917005, 1000368], [1000369, 1083732], [1083733, 1167096], [1167097, 1250460], [1250461, 1333824], [1333825, 1417188], [1417189, 1500552], [1500553, 1583916], [1583917, 1667289]] ERR6133453 file size 365430 ERR6133453 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133453 ERR6133453_1.fastq Input file: ERR6133453_1.fastq trimmed: ERR6133453-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:21:52 2024 >> started Sat Dec 7 06:21:53 2024 >> done (1.123s) 1667289 reads processed; of these: 237 ( 0.01%) short reads filtered out after trimming by size control 14 ( 0.00%) empty reads filtered out after trimming by size control 1667038 (99.98%) reads available; of these: 25104 ( 1.51%) trimmed reads available after processing 1641934 (98.49%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 24 0.00% 19 43 0.00% 20 18 0.00% 21 21 0.00% 22 19 0.00% 23 20 0.00% 24 11 0.00% 25 11 0.00% 26 8 0.00% 27 12 0.00% 28 53 0.00% 29 51 0.00% 30 23 0.00% 31 35 0.00% 32 20 0.00% 33 35 0.00% 34 21 0.00% 35 46 0.00% 36 574 0.03% 37 32 0.00% 38 52 0.00% 39 117 0.01% 40 45 0.00% 41 40 0.00% 42 12 0.00% 43 25 0.00% 44 58 0.00% 45 29 0.00% 46 34 0.00% 47 14 0.00% 48 10 0.00% 49 11 0.00% 50 30 0.00% 51 51 0.00% 52 10 0.00% 53 10 0.00% 54 9 0.00% 55 6 0.00% 56 18 0.00% 57 89 0.01% 58 13 0.00% 59 17 0.00% 60 13 0.00% 61 14 0.00% 62 1 0.00% 63 0 0.00% 64 5 0.00% 65 3 0.00% 66 5 0.00% 67 7 0.00% 68 22 0.00% 69 96 0.01% 70 8561 0.51% 71 8377 0.50% 72 8034 0.48% 73 8205 0.49% 74 8264 0.50% 75 8903 0.53% 76 7441 0.45% 77 6816 0.41% 78 7117 0.43% 79 6767 0.41% 80 6448 0.39% 81 6805 0.41% 82 7786 0.47% 83 8099 0.49% 84 6319 0.38% 85 54 0.00% 86 112 0.01% 87 190 0.01% 88 280 0.02% 89 602 0.04% 90 1314 0.08% 91 3980 0.24% 92 15461 0.93% 93 1529160 91.73% 1667038 reads passed initial QC criterion=sequence-density sequence-density=0.67 sequence-density-rank=1 fanout-score=3.55 fanout-score-rank=30 prefix-density=0.81 prefix-fanout=2.9 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=76.39 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=4.4 sequence=GTATCTTTTTCACTCAGGACTGGGTATCCATGCCAGGTGTTATACCAGTAGCTTCAGGTGGTATTCATGTTTGGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTAATAGATAAAACTAAATATGAAGAAGGTCTAAA Started job on | Dec 07 06:22:11 Started mapping on | Dec 07 06:22:11 Finished on | Dec 07 06:22:21 Mapping speed, Million of reads per hour | 600.13 Number of input reads | 1667038 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 1281744 Uniquely mapped reads % | 76.89% Average mapped length | 91.97 Number of splices: Total | 46812 Number of splices: Annotated (sjdb) | 36656 Number of splices: GT/AG | 43552 Number of splices: GC/AG | 1752 Number of splices: AT/AC | 21 Number of splices: Non-canonical | 1487 Mismatch rate per base, % | 0.56% Deletion rate per base | 0.05% Deletion average length | 1.85 Insertion rate per base | 0.02% Insertion average length | 1.74 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 250814 % of reads mapped to multiple loci | 15.05% Number of reads mapped to too many loci | 14167 % of reads mapped to too many loci | 0.85% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.11% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 134480 134480 134480 N_multimapping 250814 250814 250814 N_noFeature 80819 91747 1224812 N_ambiguous 50725 4755 116 UnstrandedReadsAssigned:1150200 PositiveStrandReadsAssigned:1185242 NegativeStrandReadsAssigned:56816 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133453 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133453-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,667,038 reads, 1,333,883 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 958 rounds 52973 ERR6133453.ke.tsv 35125 ERR6133453.se.tsv 88098 total ==> ERR6133453.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 57 41.9029 PNS24243 293 194 0 0 KQK14069 1603 1504 25 16.7655 KQK14071 474 375 0 0 ==> ERR6133453.se.tsv <== BRADI_1g14170v3 25 BRADI_1g53295v3 28 BRADI_1g59795v3 23 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 21 BRADI_1g74790v3 9 BRADI_1g09890v3 0 BRADI_1g77505v3 48 BRADI_1g48960v3 0 ERR6133453 completed mapping pipeline successfully