Starting /dee2/code/volunteer_pipeline.sh ERR6133464 current disk space = 1545181798400 free memory = 1418326280 ERR6133464 SRAfilesize 2e5df9d87ebdc4d12173c759367dc195 ERR6133464.sra ERR6133464.sra file validated ERR6133464 is single end ERR6133464 is conventional basespace ERR6133464 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133464_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.0745 37.0 33.0 37.0 33.0 37.0 2 36.20075 37.0 37.0 37.0 33.0 37.0 3 35.35 37.0 33.0 37.0 33.0 37.0 4 34.981 37.0 37.0 37.0 33.0 37.0 5 34.7195 37.0 33.0 37.0 27.0 37.0 6 35.2495 37.0 37.0 37.0 33.0 37.0 7 36.59675 37.0 37.0 40.0 33.0 40.0 8 36.72875 37.0 37.0 40.0 33.0 40.0 9 36.93325 37.0 37.0 40.0 33.0 40.0 10-11 36.86375 37.0 37.0 40.0 33.0 40.0 12-13 36.869249999999994 37.0 37.0 40.0 33.0 40.0 14-15 36.7645 37.0 37.0 40.0 33.0 40.0 16-17 36.672875000000005 37.0 37.0 40.0 33.0 40.0 18-19 36.34287500000001 37.0 37.0 40.0 33.0 40.0 20-21 36.25 37.0 37.0 40.0 33.0 40.0 22-23 35.912625 37.0 35.0 40.0 33.0 40.0 24-25 36.121125 37.0 35.0 40.0 33.0 40.0 26-27 36.2545 37.0 37.0 40.0 33.0 40.0 28-29 36.275125 37.0 37.0 40.0 33.0 40.0 30-31 36.285625 37.0 37.0 40.0 33.0 40.0 32-33 36.24525 37.0 37.0 40.0 33.0 40.0 34-35 36.245999999999995 37.0 37.0 40.0 33.0 40.0 36-37 36.09925 37.0 35.0 40.0 33.0 40.0 38-39 36.071749999999994 37.0 37.0 40.0 33.0 40.0 40-41 35.963625 37.0 33.0 40.0 33.0 40.0 42-43 35.876875 37.0 33.0 40.0 33.0 40.0 44-45 35.68275 37.0 33.0 38.5 33.0 40.0 46-47 35.667375 37.0 33.0 37.0 33.0 40.0 48-49 35.650000000000006 37.0 33.0 37.0 33.0 40.0 50-51 35.50475 37.0 33.0 37.0 33.0 40.0 52-53 35.148250000000004 37.0 33.0 37.0 33.0 40.0 54-55 35.073125000000005 37.0 33.0 37.0 33.0 40.0 56-57 34.95825000000001 37.0 33.0 37.0 33.0 38.5 58-59 34.42825 37.0 33.0 37.0 27.0 37.0 60-61 34.602125 37.0 33.0 37.0 30.0 37.0 62-63 34.492374999999996 37.0 33.0 37.0 27.0 37.0 64-65 34.436875 37.0 33.0 37.0 27.0 37.0 66-67 34.118875 37.0 33.0 37.0 27.0 37.0 68-69 33.3725 35.0 33.0 37.0 27.0 37.0 70-71 33.592972389558234 35.0 33.0 37.0 27.0 37.0 72-73 33.96565997193525 37.0 33.0 37.0 27.0 37.0 74-75 33.97452242085755 37.0 33.0 37.0 27.0 37.0 76-77 33.92476819343452 37.0 33.0 37.0 27.0 37.0 78-79 33.86791868262456 37.0 33.0 37.0 27.0 37.0 80-81 33.762036851439206 37.0 33.0 37.0 27.0 37.0 82-83 33.63115751538126 37.0 33.0 37.0 27.0 37.0 84-85 33.54574907123563 37.0 33.0 37.0 27.0 37.0 86-87 33.38676707907477 35.0 33.0 37.0 27.0 37.0 88-89 33.401963421194196 37.0 33.0 37.0 27.0 37.0 90-91 33.23359332974718 35.0 33.0 37.0 27.0 37.0 92-93 33.282006455083376 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 12.0 21 12.0 22 16.0 23 25.0 24 29.0 25 26.0 26 48.0 27 51.0 28 58.0 29 84.0 30 110.0 31 138.0 32 155.0 33 209.0 34 283.0 35 497.0 36 853.0 37 836.0 38 537.0 39 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 89.925 2.15 2.4 5.525 2 73.275 15.275 7.1 4.35 3 37.375 40.1 12.575 9.950000000000001 4 33.7 29.349999999999998 18.825 18.125 5 23.674999999999997 31.474999999999998 26.575 18.275 6 20.974999999999998 37.675 26.424999999999997 14.924999999999999 7 36.275 29.375 20.4 13.950000000000001 8 32.025 30.275000000000002 22.875 14.825 9 26.924999999999997 28.625 27.1 17.349999999999998 10-11 24.975 29.25 27.987499999999997 17.7875 12-13 27.075 26.487500000000004 29.075 17.3625 14-15 21.912499999999998 30.1375 29.5 18.45 16-17 24.1125 30.9625 26.900000000000002 18.025 18-19 23.125 27.0625 29.5375 20.275000000000002 20-21 23.892919689767325 25.719289467100324 29.947460595446586 20.440330247685765 22-23 24.887500000000003 24.6875 29.862499999999997 20.5625 24-25 24.9375 25.087500000000002 29.425 20.549999999999997 26-27 24.3125 26.200000000000003 30.837500000000002 18.65 28-29 24.5 26.924999999999997 28.8375 19.7375 30-31 26.174999999999997 25.4625 28.875 19.4875 32-33 24.375 26.5125 28.9875 20.125 34-35 24.825 26.0375 29.125 20.0125 36-37 24.825 25.85 28.849999999999998 20.474999999999998 38-39 25.5 25.587500000000002 29.475 19.4375 40-41 25.6125 26.200000000000003 27.8125 20.375 42-43 23.84827240861292 28.617926890335504 27.41612418627942 20.11767651477216 44-45 23.377922240280036 26.690836354544317 30.24128016002 19.689961245155644 46-47 23.4875 25.2375 28.999999999999996 22.275 48-49 23.5875 25.662499999999998 30.45 20.3 50-51 23.724999999999998 27.237499999999997 28.962500000000002 20.075000000000003 52-53 24.592833876221498 27.023302430468554 27.787521924329745 20.596341768980206 54-55 22.927865983247905 28.116014501812725 30.61632704088011 18.33979247405926 56-57 24.4 27.212500000000002 29.95 18.4375 58-59 23.375 26.275 30.025000000000002 20.325 60-61 23.674999999999997 25.8625 30.25 20.2125 62-63 21.712500000000002 27.55 32.1 18.637500000000003 64-65 23.4625 26.7125 30.075000000000003 19.75 66-67 23.974999999999998 27.2625 30.2875 18.475 68-69 22.7375 27.037499999999998 30.112499999999997 20.1125 70-71 23.13376753507014 25.826653306613228 29.646793587174347 21.392785571142284 72-73 24.883103753317325 25.38860103626943 29.6221407809933 20.10615442941994 74-75 23.308654582588716 27.20194026040337 29.7676793464386 19.721725810569314 76-77 22.440284054228535 26.881859264041317 30.25177533892834 20.426081342801808 78-79 22.644951140065146 26.1628664495114 32.20846905537459 18.98371335504886 80-81 23.152774125690247 27.58348672100973 30.738890349723903 18.524848803576123 82-83 23.094259578417077 26.660479915153125 30.942595784170756 19.302664722259046 84-85 23.29263383872266 25.050315309271436 31.410170401180732 20.246880450825174 86-87 22.673480365788055 26.143087681549222 31.75094136632598 19.43249058633674 88-89 21.261430876815492 28.267885960193652 31.65680473372781 18.813878429263045 90-91 24.394835933297472 27.447552447552447 29.679935449166216 18.477676169983862 92-93 22.229693383539537 29.222700376546527 29.82786444324906 18.719741796664874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 5.5 18 6.5 19 1.0 20 0.0 21 1.0 22 2.5 23 5.0 24 6.0 25 7.0 26 11.0 27 16.5 28 23.5 29 28.0 30 34.0 31 53.5 32 71.0 33 86.0 34 106.5 35 123.0 36 155.5 37 193.5 38 195.0 39 178.5 40 187.5 41 209.5 42 233.5 43 247.5 44 223.0 45 192.0 46 203.0 47 188.5 48 163.0 49 161.5 50 143.0 51 124.0 52 118.5 53 118.0 54 102.5 55 72.5 56 52.5 57 52.0 58 46.0 59 33.0 60 31.5 61 29.5 62 25.5 63 23.0 64 24.0 65 25.0 66 14.5 67 11.0 68 10.0 69 10.0 70 9.5 71 7.0 72 5.5 73 4.0 74 3.0 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.075 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.15 44-45 0.0125 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.22499999999999998 54-55 0.0125 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 16.0 71 18.0 72 19.0 73 18.0 74 24.0 75 21.0 76 23.0 77 11.0 78 25.0 79 16.0 80 12.0 81 16.0 82 19.0 83 27.0 84 17.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3718.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.75 #Duplication Level Percentage of deduplicated Percentage of total 1 94.35158501440922 81.85 2 3.544668587896253 6.15 3 0.7204610951008645 1.875 4 0.46109510086455335 1.6 5 0.20172910662824206 0.8750000000000001 6 0.05763688760806917 0.3 7 0.05763688760806917 0.35000000000000003 8 0.028818443804034585 0.2 9 0.20172910662824206 1.575 >10 0.37463976945244953 5.225 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 30 0.75 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 29 0.7250000000000001 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 21 0.525 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 20 0.5 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 15 0.375 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 14 0.35000000000000003 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 13 0.325 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 13 0.325 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 12 0.3 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 11 0.27499999999999997 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 11 0.27499999999999997 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 10 0.25 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 10 0.25 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 9 0.22499999999999998 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 9 0.22499999999999998 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 9 0.22499999999999998 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 9 0.22499999999999998 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 9 0.22499999999999998 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 9 0.22499999999999998 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 9 0.22499999999999998 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 8 0.2 No Hit GGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTG 7 0.17500000000000002 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 7 0.17500000000000002 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 6 0.15 No Hit GGAGATCGAGTTGTTACTTGAGAGTTTGTAACCCTTTATCATGCCATGTC 6 0.15 No Hit GGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTC 5 0.125 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 5 0.125 No Hit GGAGGAGAGCGGCGGCGGTCTGTTCAAGATGGCTCAGGGCTTCATGAAGT 5 0.125 No Hit GGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGA 5 0.125 No Hit GGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCAT 5 0.125 No Hit GGGAAATTAACAGTTGGAAAGGGCGATCGGTCTTGATCCCTCTGTGTTTC 5 0.125 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128859 READS because READLEN < 1 Read 128859 spots for ERR6133464.sra Written 128859 spots for ERR6133464.sra Rejected 128873 READS because READLEN < 1 Read 128873 spots for ERR6133464.sra Written 128873 spots for ERR6133464.sra SRR ids: ['ERR6133464.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_8p4xqzcv ERR6133464.sra spots: 2577194 blocks: [[1, 128859], [128860, 257718], [257719, 386577], [386578, 515436], [515437, 644295], [644296, 773154], [773155, 902013], [902014, 1030872], [1030873, 1159731], [1159732, 1288590], [1288591, 1417449], [1417450, 1546308], [1546309, 1675167], [1675168, 1804026], [1804027, 1932885], [1932886, 2061744], [2061745, 2190603], [2190604, 2319462], [2319463, 2448321], [2448322, 2577194]] ERR6133464 file size 565205 ERR6133464 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133464 ERR6133464_1.fastq Input file: ERR6133464_1.fastq trimmed: ERR6133464-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:35:30 2024 >> started Sat Dec 7 06:35:36 2024 >> done (6.482s) 2577194 reads processed; of these: 104 ( 0.00%) short reads filtered out after trimming by size control 10 ( 0.00%) empty reads filtered out after trimming by size control 2577080 (100.00%) reads available; of these: 36714 ( 1.42%) trimmed reads available after processing 2540366 (98.58%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 13 0.00% 19 31 0.00% 20 11 0.00% 21 10 0.00% 22 14 0.00% 23 6 0.00% 24 3 0.00% 25 2 0.00% 26 7 0.00% 27 8 0.00% 28 14 0.00% 29 63 0.00% 30 5 0.00% 31 16 0.00% 32 16 0.00% 33 7 0.00% 34 20 0.00% 35 101 0.00% 36 290 0.01% 37 17 0.00% 38 18 0.00% 39 69 0.00% 40 61 0.00% 41 28 0.00% 42 10 0.00% 43 12 0.00% 44 9 0.00% 45 13 0.00% 46 7 0.00% 47 7 0.00% 48 3 0.00% 49 11 0.00% 50 9 0.00% 51 31 0.00% 52 9 0.00% 53 5 0.00% 54 7 0.00% 55 4 0.00% 56 5 0.00% 57 10 0.00% 58 9 0.00% 59 8 0.00% 60 22 0.00% 61 10 0.00% 62 3 0.00% 63 5 0.00% 64 1 0.00% 65 4 0.00% 66 6 0.00% 67 17 0.00% 68 41 0.00% 69 134 0.01% 70 13822 0.54% 71 13774 0.53% 72 14477 0.56% 73 13002 0.50% 74 13303 0.52% 75 13192 0.51% 76 12126 0.47% 77 12669 0.49% 78 13645 0.53% 79 15186 0.59% 80 13544 0.53% 81 14261 0.55% 82 15335 0.60% 83 16772 0.65% 84 13201 0.51% 85 92 0.00% 86 148 0.01% 87 280 0.01% 88 444 0.02% 89 865 0.03% 90 1912 0.07% 91 5986 0.23% 92 23650 0.92% 93 2334152 90.57% 2577080 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=11.58 fanout-score-rank=12 prefix-density=0.46 prefix-fanout=6.1 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=25 fanout-score=201.17 fanout-score-rank=1 prefix-density=0.51 prefix-fanout=8.3 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTTCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAATAGATACAGGGTCTCAAACCTTGCTATAGAGTTTTTGCTT Started job on | Dec 07 06:35:56 Started mapping on | Dec 07 06:35:56 Finished on | Dec 07 06:36:16 Mapping speed, Million of reads per hour | 463.87 Number of input reads | 2577080 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 2052192 Uniquely mapped reads % | 79.63% Average mapped length | 91.17 Number of splices: Total | 84755 Number of splices: Annotated (sjdb) | 70740 Number of splices: GT/AG | 80918 Number of splices: GC/AG | 2285 Number of splices: AT/AC | 38 Number of splices: Non-canonical | 1514 Mismatch rate per base, % | 0.45% Deletion rate per base | 0.04% Deletion average length | 1.92 Insertion rate per base | 0.02% Insertion average length | 1.75 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 467888 % of reads mapped to multiple loci | 18.16% Number of reads mapped to too many loci | 18830 % of reads mapped to too many loci | 0.73% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.42% % of reads unmapped: other | 0.06% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 57000 57000 57000 N_multimapping 467888 467888 467888 N_noFeature 136279 155571 1959542 N_ambiguous 82078 8670 332 UnstrandedReadsAssigned:1833835 PositiveStrandReadsAssigned:1887951 NegativeStrandReadsAssigned:92318 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133464 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133464-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,577,080 reads, 2,174,367 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 991 rounds 52973 ERR6133464.ke.tsv 35125 ERR6133464.se.tsv 88098 total ==> ERR6133464.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 73 33.8236 PNS24243 293 194 0 0 KQK14069 1603 1504 55 23.247 KQK14071 474 375 0 0 ==> ERR6133464.se.tsv <== BRADI_1g14170v3 55 BRADI_1g53295v3 37 BRADI_1g59795v3 17 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 24 BRADI_1g74790v3 31 BRADI_1g09890v3 0 BRADI_1g77505v3 46 BRADI_1g48960v3 0 ERR6133464 completed mapping pipeline successfully