Starting /dee2/code/volunteer_pipeline.sh ERR6133469 current disk space = 1545094496256 free memory = 1447614200 ERR6133469 SRAfilesize 4820f335e7925bb74dabeff52efa403c ERR6133469.sra ERR6133469.sra file validated ERR6133469 is single end ERR6133469 is conventional basespace ERR6133469 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133469_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.786 37.0 33.0 37.0 33.0 37.0 2 36.2035 37.0 37.0 37.0 33.0 37.0 3 35.2645 37.0 33.0 37.0 33.0 37.0 4 34.864 37.0 33.0 37.0 33.0 37.0 5 34.742 37.0 33.0 37.0 27.0 37.0 6 35.288 37.0 37.0 37.0 33.0 37.0 7 36.6745 37.0 37.0 40.0 33.0 40.0 8 36.9145 37.0 37.0 40.0 33.0 40.0 9 37.02925 37.0 37.0 40.0 33.0 40.0 10-11 36.96 37.0 37.0 40.0 33.0 40.0 12-13 36.88475 37.0 37.0 40.0 33.0 40.0 14-15 36.750875 37.0 37.0 40.0 33.0 40.0 16-17 36.519875 37.0 37.0 40.0 33.0 40.0 18-19 36.290875 37.0 37.0 40.0 33.0 40.0 20-21 36.16475 37.0 35.0 40.0 33.0 40.0 22-23 35.744125 37.0 33.0 40.0 33.0 40.0 24-25 35.82325 37.0 33.0 40.0 33.0 40.0 26-27 36.102625 37.0 33.0 40.0 33.0 40.0 28-29 35.95075 37.0 33.0 40.0 33.0 40.0 30-31 36.012249999999995 37.0 33.0 40.0 33.0 40.0 32-33 35.888999999999996 37.0 33.0 40.0 33.0 40.0 34-35 35.814 37.0 33.0 40.0 33.0 40.0 36-37 35.819875 37.0 33.0 40.0 33.0 40.0 38-39 35.708 37.0 33.0 40.0 33.0 40.0 40-41 35.686375 37.0 33.0 40.0 33.0 40.0 42-43 35.622125 37.0 33.0 40.0 33.0 40.0 44-45 35.3635 37.0 33.0 37.0 30.0 40.0 46-47 35.286249999999995 37.0 33.0 37.0 30.0 40.0 48-49 35.382125 37.0 33.0 37.0 33.0 40.0 50-51 35.072125 37.0 33.0 37.0 27.0 40.0 52-53 34.772125 37.0 33.0 37.0 27.0 40.0 54-55 34.771125 37.0 33.0 37.0 30.0 38.5 56-57 34.658875 37.0 33.0 37.0 27.0 37.0 58-59 34.154624999999996 37.0 33.0 37.0 27.0 37.0 60-61 34.334625 37.0 33.0 37.0 27.0 37.0 62-63 34.064375 37.0 33.0 37.0 27.0 37.0 64-65 33.879625000000004 37.0 33.0 37.0 27.0 37.0 66-67 33.759625 37.0 33.0 37.0 27.0 37.0 68-69 33.00475 35.0 33.0 37.0 27.0 37.0 70-71 33.12205761523046 35.0 33.0 37.0 27.0 37.0 72-73 33.514966460769 37.0 33.0 37.0 27.0 37.0 74-75 33.52979045929759 37.0 33.0 37.0 27.0 37.0 76-77 33.65338971664589 37.0 33.0 37.0 27.0 37.0 78-79 33.57751770995722 37.0 33.0 37.0 27.0 37.0 80-81 33.420990715725765 37.0 33.0 37.0 27.0 37.0 82-83 33.32325401557301 37.0 33.0 37.0 27.0 37.0 84-85 33.14902800737471 33.0 33.0 37.0 27.0 37.0 86-87 32.988401733367326 33.0 33.0 37.0 27.0 37.0 88-89 33.07838388988019 33.0 33.0 37.0 27.0 37.0 90-91 32.78103492225338 33.0 33.0 37.0 27.0 37.0 92-93 32.836732092786136 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 17.0 21 20.0 22 25.0 23 29.0 24 19.0 25 49.0 26 52.0 27 55.0 28 68.0 29 93.0 30 115.0 31 122.0 32 180.0 33 199.0 34 339.0 35 529.0 36 812.0 37 849.0 38 417.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 89.35 2.375 2.725 5.55 2 72.6 16.400000000000002 6.9750000000000005 4.025 3 36.025 40.35 12.575 11.05 4 35.075 28.375 17.9 18.65 5 23.674999999999997 33.425 24.675 18.224999999999998 6 20.575 39.875 23.849999999999998 15.7 7 36.225 29.15 19.275000000000002 15.35 8 33.25 27.05 22.5 17.2 9 27.200000000000003 29.2 25.7 17.9 10-11 26.474999999999998 28.512500000000003 25.924999999999997 19.0875 12-13 28.6375 24.85 26.650000000000002 19.8625 14-15 23.5125 29.65 27.0125 19.825 16-17 25.55 30.2 25.7125 18.5375 18-19 24.25 26.687499999999996 26.125 22.9375 20-21 25.31898924193145 26.482361771328495 26.770077558168627 21.428571428571427 22-23 28.199999999999996 23.05 27.187499999999996 21.5625 24-25 26.950000000000003 23.45 27.6125 21.987499999999997 26-27 26.237500000000004 25.825 29.549999999999997 18.387500000000003 28-29 26.8125 26.2875 25.6 21.3 30-31 28.537499999999998 25.662499999999998 25.2875 20.5125 32-33 24.925 27.075 27.6125 20.3875 34-35 27.85 24.625 26.200000000000003 21.325 36-37 25.374999999999996 24.55 27.3375 22.7375 38-39 26.200000000000003 25.1 29.4 19.3 40-41 27.85 26.224999999999998 25.4875 20.4375 42-43 25.906930197648236 28.096072054040533 25.23142356767576 20.765574180635475 44-45 24.765595699462434 25.25315664458057 27.240905113139142 22.740342542817853 46-47 25.6 23.0875 27.55 23.7625 48-49 25.874999999999996 24.7375 28.525 20.8625 50-51 26.5125 25.124999999999996 27.6375 20.724999999999998 52-53 25.849742882227517 27.605669133324973 24.771102470839082 21.773485513608428 54-55 23.952470293933708 29.04315196998124 27.10444027517198 19.89993746091307 56-57 25.8 26.150000000000002 27.6875 20.3625 58-59 25.224999999999998 24.349999999999998 27.737499999999997 22.6875 60-61 25.362499999999997 24.15 29.012500000000003 21.475 62-63 23.549999999999997 25.7625 30.525000000000002 20.1625 64-65 24.7375 27.4125 26.825 21.025 66-67 25.162499999999998 27.0875 27.775 19.975 68-69 24.128016002000248 25.9407425928241 27.778472309038634 22.152769096137018 70-71 26.539039039039036 25.06256256256256 26.5015015015015 21.896896896896898 72-73 26.222110804712962 24.091250940085235 28.16495362246177 21.521684632740033 74-75 22.87401080266298 28.564250722271073 27.923627684964202 20.638110790101745 76-77 23.8755197177775 25.33702910419554 28.713619755575152 22.07383142245181 78-79 26.449412656309207 24.49160035366932 28.31880762915246 20.740179360869014 80-81 25.548092763908247 27.19553922189836 29.273856291978202 17.982511722215182 82-83 25.7433290978399 23.989834815756037 28.767471410419315 21.499364675984754 84-85 25.452229299363054 22.777070063694268 30.178343949044585 21.59235668789809 86-87 22.814172826918174 27.083864389497837 30.818251338261533 19.283711445322457 88-89 23.591639051746114 28.804486362477693 28.38388988019373 19.219984705582462 90-91 26.650522559265866 24.891664542442008 29.25057354065766 19.207239357634464 92-93 23.591639051746114 29.39077236808565 27.5172062197298 19.50038236043844 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 6.5 18 8.5 19 2.5 20 1.5 21 2.5 22 4.0 23 6.0 24 7.0 25 5.5 26 5.5 27 13.0 28 20.5 29 22.0 30 28.0 31 29.0 32 26.5 33 38.5 34 53.5 35 68.5 36 108.5 37 131.5 38 129.5 39 128.5 40 133.5 41 160.0 42 177.5 43 179.5 44 194.0 45 189.0 46 209.5 47 215.5 48 173.5 49 156.0 50 160.0 51 183.0 52 184.5 53 184.5 54 165.5 55 115.5 56 76.5 57 60.5 58 60.5 59 61.5 60 55.5 61 47.5 62 47.0 63 46.0 64 39.0 65 34.0 66 27.0 67 24.5 68 23.5 69 13.5 70 10.5 71 12.0 72 11.5 73 7.0 74 3.0 75 2.0 76 0.5 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.075 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.075 44-45 0.0125 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.3375 54-55 0.0625 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0125 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 8.0 71 0.0 72 6.0 73 2.0 74 7.0 75 6.0 76 5.0 77 5.0 78 5.0 79 9.0 80 3.0 81 6.0 82 6.0 83 5.0 84 4.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3923.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.375 #Duplication Level Percentage of deduplicated Percentage of total 1 94.44613050075873 77.8 2 3.2776934749620636 5.4 3 0.7587253414264037 1.875 4 0.3338391502276176 1.0999999999999999 5 0.2731411229135053 1.125 6 0.12139605462822459 0.6 7 0.09104704097116845 0.525 8 0.030349013657056147 0.2 9 0.06069802731411229 0.44999999999999996 >10 0.5766312594840668 9.375 >50 0.030349013657056147 1.55 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 62 1.55 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 44 1.0999999999999999 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 36 0.8999999999999999 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 34 0.8500000000000001 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 29 0.7250000000000001 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 28 0.7000000000000001 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 27 0.675 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 23 0.575 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 19 0.475 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 17 0.42500000000000004 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 16 0.4 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 13 0.325 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 13 0.325 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 12 0.3 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 12 0.3 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 12 0.3 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 10 0.25 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 10 0.25 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 10 0.25 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 10 0.25 No Hit GGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTT 9 0.22499999999999998 No Hit GGAGAGAGTGAGCATATATATTTATACGACGAATAAAAGGCTGCCACGTG 9 0.22499999999999998 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 8 0.2 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 7 0.17500000000000002 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 7 0.17500000000000002 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 6 0.15 No Hit GGATTTGAAGAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAG 6 0.15 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 6 0.15 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 6 0.15 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 5 0.125 No Hit GGGTCGAAATATGGCTTTCAAATTAAGTTCCGAATTAGTAGATGCTGCCA 5 0.125 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 5 0.125 No Hit GGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAA 5 0.125 No Hit GGGACAGTGATGGATTTCTTCATAAGGACGATGCGAGAGGCATATATGAT 5 0.125 No Hit GATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGGG 5 0.125 No Hit GGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAA 5 0.125 No Hit GGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGT 5 0.125 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0125 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCAAT 20 0.0027517793 65.11875 1 >>END_MODULE Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179573 READS because READLEN < 1 Read 179573 spots for ERR6133469.sra Written 179573 spots for ERR6133469.sra Rejected 179576 READS because READLEN < 1 Read 179576 spots for ERR6133469.sra Written 179576 spots for ERR6133469.sra SRR ids: ['ERR6133469.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_n1qu05np ERR6133469.sra spots: 3591463 blocks: [[1, 179573], [179574, 359146], [359147, 538719], [538720, 718292], [718293, 897865], [897866, 1077438], [1077439, 1257011], [1257012, 1436584], [1436585, 1616157], [1616158, 1795730], [1795731, 1975303], [1975304, 2154876], [2154877, 2334449], [2334450, 2514022], [2514023, 2693595], [2693596, 2873168], [2873169, 3052741], [3052742, 3232314], [3232315, 3411887], [3411888, 3591463]] ERR6133469 file size 795074 ERR6133469 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133469 ERR6133469_1.fastq Input file: ERR6133469_1.fastq trimmed: ERR6133469-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:43:27 2024 >> started Sat Dec 7 06:43:29 2024 >> done (2.058s) 3591463 reads processed; of these: 189 ( 0.01%) short reads filtered out after trimming by size control 10 ( 0.00%) empty reads filtered out after trimming by size control 3591264 (99.99%) reads available; of these: 62941 ( 1.75%) trimmed reads available after processing 3528323 (98.25%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 13 0.00% 19 38 0.00% 20 23 0.00% 21 18 0.00% 22 24 0.00% 23 12 0.00% 24 14 0.00% 25 5 0.00% 26 9 0.00% 27 10 0.00% 28 14 0.00% 29 32 0.00% 30 11 0.00% 31 18 0.00% 32 28 0.00% 33 21 0.00% 34 15 0.00% 35 82 0.00% 36 571 0.02% 37 16 0.00% 38 23 0.00% 39 77 0.00% 40 48 0.00% 41 35 0.00% 42 7 0.00% 43 17 0.00% 44 9 0.00% 45 11 0.00% 46 8 0.00% 47 8 0.00% 48 5 0.00% 49 13 0.00% 50 4 0.00% 51 47 0.00% 52 7 0.00% 53 6 0.00% 54 7 0.00% 55 13 0.00% 56 8 0.00% 57 9 0.00% 58 14 0.00% 59 10 0.00% 60 18 0.00% 61 10 0.00% 62 1 0.00% 63 5 0.00% 64 9 0.00% 65 9 0.00% 66 17 0.00% 67 14 0.00% 68 28 0.00% 69 59 0.00% 70 5153 0.14% 71 4920 0.14% 72 5209 0.15% 73 4901 0.14% 74 4968 0.14% 75 4977 0.14% 76 4522 0.13% 77 4636 0.13% 78 5212 0.15% 79 5601 0.16% 80 5337 0.15% 81 5664 0.16% 82 6265 0.17% 83 6039 0.17% 84 5356 0.15% 85 207 0.01% 86 327 0.01% 87 501 0.01% 88 944 0.03% 89 1834 0.05% 90 3908 0.11% 91 11231 0.31% 92 41175 1.15% 93 3450847 96.09% 3591264 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=4.90 fanout-score-rank=20 prefix-density=0.73 prefix-fanout=3.5 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.04 sequence-density-rank=22 fanout-score=73.88 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=10.9 sequence=TGAAGAAGAATTGGAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTTCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGA Started job on | Dec 07 06:43:48 Started mapping on | Dec 07 06:43:49 Finished on | Dec 07 06:43:55 Mapping speed, Million of reads per hour | 2154.76 Number of input reads | 3591264 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 2641593 Uniquely mapped reads % | 73.56% Average mapped length | 92.27 Number of splices: Total | 233661 Number of splices: Annotated (sjdb) | 200425 Number of splices: GT/AG | 228523 Number of splices: GC/AG | 3753 Number of splices: AT/AC | 69 Number of splices: Non-canonical | 1316 Mismatch rate per base, % | 0.46% Deletion rate per base | 0.04% Deletion average length | 1.78 Insertion rate per base | 0.02% Insertion average length | 1.65 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 872878 % of reads mapped to multiple loci | 24.31% Number of reads mapped to too many loci | 24774 % of reads mapped to too many loci | 0.69% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.39% % of reads unmapped: other | 0.06% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 76793 76793 76793 N_multimapping 872878 872878 872878 N_noFeature 147437 172204 2530501 N_ambiguous 98869 12593 320 UnstrandedReadsAssigned:2395287 PositiveStrandReadsAssigned:2456796 NegativeStrandReadsAssigned:110772 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133469 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133469-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,591,264 reads, 3,118,470 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,121 rounds 52973 ERR6133469.ke.tsv 35125 ERR6133469.se.tsv 88098 total ==> ERR6133469.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 109 33.1209 PNS24243 293 194 0 0 KQK14069 1603 1504 35 9.70174 KQK14071 474 375 0 0 ==> ERR6133469.se.tsv <== BRADI_1g14170v3 35 BRADI_1g53295v3 43 BRADI_1g59795v3 26 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 47 BRADI_1g74790v3 41 BRADI_1g09890v3 0 BRADI_1g77505v3 94 BRADI_1g48960v3 0 ERR6133469 completed mapping pipeline successfully