Starting /dee2/code/volunteer_pipeline.sh ERR6133474 current disk space = 1544957952000 free memory = 1603148140 ERR6133474 SRAfilesize 00e04da5fc5a194024991320c24a66a0 ERR6133474.sra ERR6133474.sra file validated ERR6133474 is single end ERR6133474 is conventional basespace ERR6133474 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133474_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.0235 37.0 33.0 37.0 33.0 37.0 2 36.31275 37.0 37.0 37.0 33.0 37.0 3 35.2745 37.0 33.0 37.0 33.0 37.0 4 34.8205 37.0 33.0 37.0 33.0 37.0 5 34.6895 37.0 33.0 37.0 27.0 37.0 6 35.25525 37.0 37.0 37.0 33.0 37.0 7 36.7725 37.0 37.0 40.0 33.0 40.0 8 36.86125 37.0 37.0 40.0 33.0 40.0 9 36.938 37.0 37.0 40.0 33.0 40.0 10-11 36.947625 37.0 37.0 40.0 33.0 40.0 12-13 36.851124999999996 37.0 37.0 40.0 33.0 40.0 14-15 36.807125 37.0 37.0 40.0 33.0 40.0 16-17 36.711375000000004 37.0 37.0 40.0 33.0 40.0 18-19 36.45825 37.0 37.0 40.0 33.0 40.0 20-21 36.27575 37.0 37.0 40.0 33.0 40.0 22-23 35.89475 37.0 33.0 40.0 33.0 40.0 24-25 36.123999999999995 37.0 35.0 40.0 33.0 40.0 26-27 36.2395 37.0 35.0 40.0 33.0 40.0 28-29 36.20725 37.0 37.0 40.0 33.0 40.0 30-31 36.309875 37.0 37.0 40.0 33.0 40.0 32-33 36.17 37.0 37.0 40.0 33.0 40.0 34-35 36.083 37.0 37.0 40.0 33.0 40.0 36-37 36.12375 37.0 37.0 40.0 33.0 40.0 38-39 35.987375 37.0 35.0 40.0 33.0 40.0 40-41 35.926125 37.0 33.0 40.0 33.0 40.0 42-43 35.794875 37.0 33.0 40.0 33.0 40.0 44-45 35.51225 37.0 33.0 38.5 33.0 40.0 46-47 35.544375 37.0 33.0 37.0 33.0 40.0 48-49 35.57875 37.0 33.0 37.0 33.0 40.0 50-51 35.44775 37.0 33.0 37.0 33.0 40.0 52-53 35.19775 37.0 33.0 37.0 33.0 40.0 54-55 35.093875 37.0 33.0 37.0 33.0 40.0 56-57 35.003625 37.0 33.0 37.0 33.0 38.5 58-59 34.418375 37.0 33.0 37.0 27.0 37.0 60-61 34.761125 37.0 33.0 37.0 33.0 37.0 62-63 34.53825 37.0 33.0 37.0 27.0 37.0 64-65 34.24625 37.0 33.0 37.0 27.0 37.0 66-67 34.195750000000004 37.0 33.0 37.0 27.0 37.0 68-69 33.328374999999994 35.0 33.0 37.0 27.0 37.0 70-71 33.526552182639236 35.0 33.0 37.0 27.0 37.0 72-73 33.95873631181193 37.0 33.0 37.0 27.0 37.0 74-75 33.93260604582032 37.0 33.0 37.0 27.0 37.0 76-77 33.98496671847547 37.0 33.0 37.0 27.0 37.0 78-79 33.98188404749773 37.0 33.0 37.0 27.0 37.0 80-81 33.918621381984266 37.0 33.0 37.0 27.0 37.0 82-83 33.75063039258717 37.0 33.0 37.0 27.0 37.0 84-85 33.55335733436757 35.0 33.0 37.0 27.0 37.0 86-87 33.48688696564385 33.0 33.0 37.0 27.0 37.0 88-89 33.513506425386836 37.0 33.0 37.0 27.0 37.0 90-91 33.299632835038025 33.0 33.0 37.0 27.0 37.0 92-93 33.19603986362444 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 14.0 21 7.0 22 14.0 23 32.0 24 28.0 25 27.0 26 30.0 27 51.0 28 52.0 29 90.0 30 103.0 31 146.0 32 175.0 33 195.0 34 273.0 35 527.0 36 905.0 37 851.0 38 471.0 39 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 88.94999999999999 2.5 2.4 6.15 2 73.6 16.45 6.325 3.6249999999999996 3 37.5 38.574999999999996 14.075 9.85 4 32.15 29.65 18.975 19.225 5 26.025 30.8 25.575 17.599999999999998 6 19.8 38.925 25.074999999999996 16.2 7 35.25 29.95 19.675 15.125 8 30.275000000000002 29.975 22.400000000000002 17.349999999999998 9 24.925 29.15 28.299999999999997 17.625 10-11 25.674999999999997 27.975 28.725 17.625 12-13 27.400000000000002 27.0125 27.950000000000003 17.6375 14-15 21.1875 30.2625 30.362499999999997 18.1875 16-17 24.925 30.6875 25.162499999999998 19.225 18-19 24.3 27.325 28.1875 20.1875 20-21 25.25328330206379 26.31644777986241 28.742964352720453 19.687304565353344 22-23 26.5375 24.625 29.1875 19.650000000000002 24-25 25.7375 24.2875 29.599999999999998 20.375 26-27 25.275 25.0625 30.625000000000004 19.037499999999998 28-29 25.4625 27.6 27.725 19.2125 30-31 27.825 25.7375 27.175 19.2625 32-33 25.05 27.1375 27.287499999999998 20.525 34-35 24.1375 27.6375 27.375 20.849999999999998 36-37 25.453181647705964 23.740467558444806 28.216027003375423 22.59032379047381 38-39 26.338169084542272 25.625312656328163 29.777388694347174 18.25912956478239 40-41 25.36884221055264 25.35633908477119 28.969742435608904 20.305076269067268 42-43 24.9749373433584 28.057644110275685 27.74436090225564 19.223057644110277 44-45 24.27160185069401 25.4345379517319 30.598974615480806 19.694885582093285 46-47 25.025 24.3 27.950000000000003 22.725 48-49 24.3125 24.7875 31.4625 19.4375 50-51 24.9 26.625 28.3375 20.1375 52-53 23.547094188376754 26.189879759519037 28.106212424849698 22.15681362725451 54-55 23.17118919594848 28.248093034888083 29.3985244466675 19.182193322495937 56-57 25.328166020752597 25.640705088136016 29.84123015376922 19.18989873734217 58-59 23.375 25.4875 30.0875 21.05 60-61 25.7625 24.587500000000002 29.9875 19.662499999999998 62-63 22.8125 26.8 31.637500000000003 18.75 64-65 23.674999999999997 28.599999999999998 28.8875 18.8375 66-67 24.837500000000002 27.237499999999997 28.9875 18.9375 68-69 21.608103038639488 26.672502188320617 29.448543203701387 22.2708515693385 70-71 23.89181066867017 26.37114951164538 28.412221387427998 21.32481843225645 72-73 25.664608794254757 25.198437696862797 30.036537734660453 19.100415774222 74-75 23.094483195941663 27.799619530754597 29.663918833227648 19.441978440076095 76-77 22.99260769819016 26.051491205709915 29.378027020137647 21.577874075962274 78-79 24.50339612969371 25.297962322183775 30.552351659618093 19.64628988850442 80-81 22.173464119772845 29.36241610738255 30.588538977800724 17.875580795043884 82-83 23.397894189522944 26.49161575458209 28.480436760691536 21.63005329520343 84-85 23.615656499541828 25.526901426888337 31.06427542872104 19.793166644848803 86-87 21.17755048518227 25.911355887752425 32.717020718594284 20.19407290847102 88-89 21.49226330972987 28.16679779701023 31.02543928665093 19.31549960660897 90-91 26.003147128245473 26.750590086546026 29.019145030159976 18.227117755048518 92-93 22.410175714660372 29.346970889063726 29.67479674796748 18.568056648308417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 10.5 18 12.0 19 3.0 20 3.5 21 4.0 22 3.5 23 3.0 24 3.5 25 6.0 26 6.0 27 13.0 28 19.5 29 19.0 30 36.5 31 49.5 32 58.0 33 80.5 34 87.0 35 98.0 36 120.0 37 140.0 38 167.0 39 193.5 40 206.5 41 205.0 42 201.5 43 204.0 44 203.0 45 201.0 46 216.5 47 190.0 48 155.0 49 166.5 50 145.5 51 126.5 52 128.0 53 122.0 54 139.0 55 106.5 56 60.5 57 61.0 58 58.0 59 49.0 60 36.5 61 30.5 62 27.5 63 28.0 64 23.0 65 15.0 66 18.0 67 17.5 68 12.5 69 9.5 70 7.0 71 6.0 72 6.5 73 6.0 74 3.5 75 1.5 76 0.5 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0625 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0125 38-39 0.05 40-41 0.025 42-43 0.25 44-45 0.0375 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.2 54-55 0.0375 56-57 0.0125 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0375 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 14.0 71 14.0 72 7.0 73 14.0 74 17.0 75 8.0 76 6.0 77 9.0 78 19.0 79 10.0 80 16.0 81 15.0 82 9.0 83 16.0 84 13.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3813.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.55 #Duplication Level Percentage of deduplicated Percentage of total 1 93.91278013325257 77.525 2 3.785584494245912 6.25 3 0.7571168988491823 1.875 4 0.4239854633555421 1.4000000000000001 5 0.21199273167777105 0.8750000000000001 6 0.18170805572380377 0.8999999999999999 7 0.09085402786190189 0.525 8 0.06056935190793458 0.4 9 0.03028467595396729 0.22499999999999998 >10 0.514839491217444 7.925 >50 0.03028467595396729 2.1 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 84 2.1 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 34 0.8500000000000001 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 26 0.65 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 25 0.625 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 24 0.6 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 24 0.6 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 21 0.525 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 21 0.525 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 19 0.475 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 18 0.44999999999999996 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 16 0.4 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 16 0.4 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 14 0.35000000000000003 No Hit GGAAAAGAGGGGTTACTTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTT 13 0.325 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 12 0.3 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 12 0.3 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 11 0.27499999999999997 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 11 0.27499999999999997 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 9 0.22499999999999998 No Hit GGGAAAAGAGGGGTTACTTTTTTTTCATTTTTCCCTTAAAAGATAGGCTT 8 0.2 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 8 0.2 No Hit GGGTTACTTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGA 7 0.17500000000000002 No Hit CAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATAT 7 0.17500000000000002 No Hit GGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTCCGTCCAAGGC 7 0.17500000000000002 No Hit GGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCA 6 0.15 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 6 0.15 No Hit GGGGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCT 6 0.15 No Hit GGGGTTACTTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGG 6 0.15 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 6 0.15 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 6 0.15 No Hit GGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTC 5 0.125 No Hit GGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACGA 5 0.125 No Hit GGGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACG 5 0.125 No Hit CAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGG 5 0.125 No Hit GGGTGTGAGCTGGAGAGACGATCGGGTCTCTCAGCCGGCGTCTTCATCAG 5 0.125 No Hit GGAGATCGAGTTGTTACTTGAGAGTTTGTAACCCTTTATCATGCCATGTC 5 0.125 No Hit CACGAACGTAATGCTCACAACTTCCCTCTAGATCTAGCTGCTCTTGAAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0875 0.0 0.0 0.0 0.0 38-39 0.175 0.0 0.0 0.0 0.0 40-41 0.1875 0.0 0.0 0.0 0.0 42-43 0.2 0.0 0.0 0.0 0.0 44-45 0.2 0.0 0.0 0.0 0.0 46-47 0.2 0.0 0.0 0.0 0.0 48-49 0.21250000000000002 0.0 0.0 0.0 0.0 50-51 0.225 0.0 0.0 0.0 0.0 52-53 0.225 0.0 0.0 0.0 0.0 54-55 0.225 0.0 0.0 0.0 0.0 56-57 0.225 0.0 0.0 0.0 0.0 58-59 0.225 0.0 0.0 0.0 0.0 60-61 0.225 0.0 0.0 0.0 0.0 62-63 0.225 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.225 0.0 0.0 0.0 0.0 68-69 0.225 0.0 0.0 0.0 0.0 70-71 0.225 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247375 READS because READLEN < 1 Read 247375 spots for ERR6133474.sra Written 247375 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra Rejected 247364 READS because READLEN < 1 Read 247364 spots for ERR6133474.sra Written 247364 spots for ERR6133474.sra SRR ids: ['ERR6133474.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jyri59a1 ERR6133474.sra spots: 4947291 blocks: [[1, 247364], [247365, 494728], [494729, 742092], [742093, 989456], [989457, 1236820], [1236821, 1484184], [1484185, 1731548], [1731549, 1978912], [1978913, 2226276], [2226277, 2473640], [2473641, 2721004], [2721005, 2968368], [2968369, 3215732], [3215733, 3463096], [3463097, 3710460], [3710461, 3957824], [3957825, 4205188], [4205189, 4452552], [4452553, 4699916], [4699917, 4947291]] ERR6133474 file size 1092044 ERR6133474 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133474 ERR6133474_1.fastq Input file: ERR6133474_1.fastq trimmed: ERR6133474-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:48:58 2024 >> started Sat Dec 7 06:49:02 2024 >> done (3.661s) 4947291 reads processed; of these: 333 ( 0.01%) short reads filtered out after trimming by size control 108 ( 0.00%) empty reads filtered out after trimming by size control 4946850 (99.99%) reads available; of these: 87288 ( 1.76%) trimmed reads available after processing 4859562 (98.24%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 63 0.00% 20 20 0.00% 21 27 0.00% 22 38 0.00% 23 11 0.00% 24 12 0.00% 25 14 0.00% 26 13 0.00% 27 15 0.00% 28 45 0.00% 29 82 0.00% 30 53 0.00% 31 112 0.00% 32 159 0.00% 33 171 0.00% 34 244 0.00% 35 529 0.01% 36 12085 0.24% 37 179 0.00% 38 156 0.00% 39 254 0.01% 40 269 0.01% 41 196 0.00% 42 183 0.00% 43 167 0.00% 44 191 0.00% 45 157 0.00% 46 203 0.00% 47 186 0.00% 48 218 0.00% 49 149 0.00% 50 159 0.00% 51 170 0.00% 52 106 0.00% 53 98 0.00% 54 94 0.00% 55 82 0.00% 56 69 0.00% 57 56 0.00% 58 56 0.00% 59 56 0.00% 60 50 0.00% 61 31 0.00% 62 1 0.00% 63 5 0.00% 64 9 0.00% 65 9 0.00% 66 14 0.00% 67 16 0.00% 68 46 0.00% 69 163 0.00% 70 15067 0.30% 71 14146 0.29% 72 15251 0.31% 73 14559 0.29% 74 15321 0.31% 75 15369 0.31% 76 13732 0.28% 77 13887 0.28% 78 16080 0.33% 79 17991 0.36% 80 15891 0.32% 81 16938 0.34% 82 18445 0.37% 83 20466 0.41% 84 16920 0.34% 85 183 0.00% 86 310 0.01% 87 532 0.01% 88 963 0.02% 89 1798 0.04% 90 3798 0.08% 91 12279 0.25% 92 47179 0.95% 93 4622237 93.44% 4946850 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=5.68 fanout-score-rank=21 prefix-density=0.66 prefix-fanout=3.9 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.07 sequence-density-rank=16 fanout-score=71.41 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=19.2 sequence=TTTTTTTTTTAAGAATCCTCCATTTTTGTTCTTCCACCCATGCAATAGAGAGCAAATGGGAAAAGAGGGGTTACTTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGAGTCATGGAATAATGGTGAATTCAAATGTTTAGTTCTTTAGTATAAGAAGTATAAGAA Started job on | Dec 07 06:49:37 Started mapping on | Dec 07 06:49:37 Finished on | Dec 07 06:49:44 Mapping speed, Million of reads per hour | 2544.09 Number of input reads | 4946850 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 3544835 Uniquely mapped reads % | 71.66% Average mapped length | 91.72 Number of splices: Total | 144803 Number of splices: Annotated (sjdb) | 119096 Number of splices: GT/AG | 138481 Number of splices: GC/AG | 3516 Number of splices: AT/AC | 88 Number of splices: Non-canonical | 2718 Mismatch rate per base, % | 0.45% Deletion rate per base | 0.04% Deletion average length | 1.83 Insertion rate per base | 0.03% Insertion average length | 1.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1262658 % of reads mapped to multiple loci | 25.52% Number of reads mapped to too many loci | 51638 % of reads mapped to too many loci | 1.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.69% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 139357 139357 139357 N_multimapping 1262658 1262658 1262658 N_noFeature 267870 300667 3391805 N_ambiguous 137690 17444 511 UnstrandedReadsAssigned:3139275 PositiveStrandReadsAssigned:3226724 NegativeStrandReadsAssigned:152519 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133474 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133474-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 4,946,850 reads, 4,060,301 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,088 rounds 52973 ERR6133474.ke.tsv 35125 ERR6133474.se.tsv 88098 total ==> ERR6133474.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 133 31.6365 PNS24243 293 194 0 0 KQK14069 1603 1504 21 4.55682 KQK14071 474 375 0 0 ==> ERR6133474.se.tsv <== BRADI_1g14170v3 21 BRADI_1g53295v3 46 BRADI_1g59795v3 31 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 60 BRADI_1g74790v3 24 BRADI_1g09890v3 0 BRADI_1g77505v3 146 BRADI_1g48960v3 0 ERR6133474 completed mapping pipeline successfully