Starting /dee2/code/volunteer_pipeline.sh ERR6133477 current disk space = 1544939245568 free memory = 1451318396 ERR6133477 SRAfilesize ef70dbda67035dcf00a4eedd5dc00598 ERR6133477.sra ERR6133477.sra file validated ERR6133477 is single end ERR6133477 is conventional basespace ERR6133477 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133477_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.38075 37.0 33.0 37.0 33.0 37.0 2 36.45175 37.0 37.0 37.0 37.0 37.0 3 35.732 37.0 37.0 37.0 33.0 37.0 4 35.089 37.0 37.0 37.0 33.0 37.0 5 35.11575 37.0 37.0 37.0 33.0 37.0 6 35.48775 37.0 37.0 37.0 33.0 37.0 7 37.22625 37.0 37.0 40.0 33.0 40.0 8 37.28425 37.0 37.0 40.0 33.0 40.0 9 37.3245 37.0 37.0 40.0 33.0 40.0 10-11 37.3455 37.0 37.0 40.0 33.0 40.0 12-13 37.2405 37.0 37.0 40.0 33.0 40.0 14-15 37.203 37.0 37.0 40.0 33.0 40.0 16-17 36.987375 37.0 37.0 40.0 33.0 40.0 18-19 36.802375 37.0 37.0 40.0 33.0 40.0 20-21 36.7155 37.0 37.0 40.0 33.0 40.0 22-23 36.6465 37.0 37.0 40.0 33.0 40.0 24-25 36.525625000000005 37.0 37.0 40.0 33.0 40.0 26-27 36.833375000000004 37.0 37.0 40.0 33.0 40.0 28-29 36.824124999999995 37.0 37.0 40.0 33.0 40.0 30-31 36.6575 37.0 37.0 40.0 33.0 40.0 32-33 36.6385 37.0 37.0 40.0 33.0 40.0 34-35 36.583375000000004 37.0 37.0 40.0 33.0 40.0 36-37 36.371625 37.0 37.0 40.0 33.0 40.0 38-39 36.29474999999999 37.0 37.0 40.0 33.0 40.0 40-41 36.036874999999995 37.0 37.0 40.0 33.0 40.0 42-43 36.116749999999996 37.0 37.0 40.0 33.0 40.0 44-45 36.1505 37.0 37.0 40.0 33.0 40.0 46-47 35.99875 37.0 37.0 38.5 33.0 40.0 48-49 35.998875 37.0 37.0 37.0 33.0 40.0 50-51 35.82425 37.0 35.0 37.0 33.0 40.0 52-53 35.6075 37.0 33.0 37.0 33.0 40.0 54-55 35.456875 37.0 33.0 37.0 33.0 40.0 56-57 35.3245 37.0 33.0 37.0 33.0 40.0 58-59 34.347125 37.0 33.0 37.0 30.0 37.0 60-61 34.862125 37.0 33.0 37.0 33.0 37.0 62-63 34.77075000000001 37.0 33.0 37.0 33.0 37.0 64-65 34.7055 37.0 33.0 37.0 33.0 37.0 66-67 34.60625 37.0 33.0 37.0 33.0 37.0 68-69 33.801875 35.0 33.0 37.0 30.0 37.0 70-71 33.938884229030634 35.0 33.0 37.0 27.0 37.0 72-73 34.36136109681681 37.0 33.0 37.0 30.0 37.0 74-75 34.28914788850379 37.0 33.0 37.0 30.0 37.0 76-77 34.13354387569045 37.0 33.0 37.0 30.0 37.0 78-79 34.086914763869444 37.0 33.0 37.0 27.0 37.0 80-81 34.0051139605573 37.0 33.0 37.0 27.0 37.0 82-83 33.924655358046365 37.0 33.0 37.0 27.0 37.0 84-85 33.78248723709332 37.0 33.0 37.0 27.0 37.0 86-87 33.66361925248723 37.0 33.0 37.0 27.0 37.0 88-89 33.73285829524065 37.0 33.0 37.0 27.0 37.0 90-91 33.46907770906158 37.0 33.0 37.0 27.0 37.0 92-93 33.41422425383168 35.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 15.0 21 13.0 22 16.0 23 9.0 24 20.0 25 29.0 26 39.0 27 35.0 28 53.0 29 59.0 30 84.0 31 98.0 32 140.0 33 181.0 34 266.0 35 504.0 36 896.0 37 947.0 38 573.0 39 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 86.225 3.4750000000000005 3.075 7.225 2 70.025 17.4 7.95 4.625 3 37.2 37.025000000000006 14.625 11.15 4 31.874999999999996 28.95 19.900000000000002 19.275000000000002 5 27.400000000000002 29.625 24.875 18.099999999999998 6 21.175 36.4 24.775 17.65 7 36.65 29.299999999999997 19.375 14.674999999999999 8 30.575000000000003 28.95 23.825 16.650000000000002 9 25.75 29.45 28.749999999999996 16.05 10-11 25.974999999999998 28.549999999999997 28.299999999999997 17.175 12-13 26.5875 26.325 29.049999999999997 18.0375 14-15 21.6125 30.112499999999997 29.4375 18.8375 16-17 25.7375 30.025000000000002 26.05 18.1875 18-19 23.8375 26.2625 29.362500000000004 20.5375 20-21 25.61570196274534 25.25315664458057 29.16614576822103 19.964995624453056 22-23 26.424999999999997 23.974999999999998 28.762500000000003 20.837500000000002 24-25 24.4 25.374999999999996 29.675 20.549999999999997 26-27 25.5 24.25 30.275000000000002 19.975 28-29 24.075 27.750000000000004 28.475 19.7 30-31 27.700000000000003 25.05 27.0125 20.2375 32-33 23.962500000000002 25.45 29.875 20.7125 34-35 24.5625 27.462500000000002 28.0625 19.9125 36-37 24.45 25.4625 26.974999999999998 23.1125 38-39 28.17271589486858 24.69336670838548 29.499374217772218 17.634543178973715 40-41 25.960215188289755 24.17114975603653 29.901163518078317 19.967471537595397 42-43 24.309115918469427 28.435663373765163 28.173064899337252 19.082155808428162 44-45 23.8375 25.362499999999997 31.025000000000002 19.775000000000002 46-47 25.624999999999996 24.587500000000002 28.7375 21.05 48-49 24.875 24.6 29.6875 20.837500000000002 50-51 23.125 28.225 28.525 20.125 52-53 23.97045938164977 25.84804105645262 28.789585680310424 21.391913881587183 54-55 23.2875 26.674999999999997 30.2125 19.825 56-57 25.478184773096636 27.028378547318415 28.716089511188898 18.777347168396048 58-59 24.075 25.825 29.599999999999998 20.5 60-61 26.6 25.55 28.8875 18.9625 62-63 22.037499999999998 28.7375 30.562499999999996 18.6625 64-65 22.787499999999998 29.025000000000002 29.5875 18.6 66-67 24.825 27.287499999999998 29.312500000000004 18.575 68-69 22.4625 25.637500000000003 30.0875 21.8125 70-71 23.076923076923077 25.407166123778502 29.441242796291654 22.074668003006764 72-73 25.98076436345229 25.613768666160464 28.94203998987598 19.463426980511265 74-75 24.46917370171399 26.47735993860322 29.79022767971348 19.2632386799693 76-77 22.867114788004137 26.202171664943126 29.201137538779733 21.72957600827301 78-79 23.498762537449526 26.533802266510353 30.389475055360165 19.577960140679952 80-81 22.564102564102566 30.87442472057857 28.11308349769888 18.448389217619987 82-83 23.635639357323065 25.972646394901076 29.58438454388527 20.807329703890588 84-85 21.99731903485255 25.482573726541556 32.07774798927614 20.44235924932976 86-87 22.15649368109707 27.883839741866094 30.209733799408443 19.749932777628395 88-89 21.215380478623285 27.937617639150307 30.881957515461146 19.96504436676526 90-91 25.410056466792145 27.238504974455495 28.919064264587252 18.4323742941651 92-93 21.417047593439094 29.25517612261361 29.927399838666307 19.40037644528099 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.5 18 2.5 19 1.0 20 0.5 21 1.0 22 0.5 23 1.5 24 3.5 25 6.5 26 8.0 27 13.5 28 24.5 29 31.0 30 35.0 31 44.0 32 53.0 33 66.5 34 90.0 35 109.0 36 131.0 37 156.0 38 184.5 39 196.5 40 206.5 41 205.5 42 216.5 43 246.0 44 226.0 45 217.0 46 214.0 47 187.0 48 159.0 49 141.5 50 143.5 51 138.0 52 115.5 53 110.5 54 138.5 55 117.5 56 67.5 57 57.0 58 47.5 59 37.5 60 32.5 61 29.0 62 30.5 63 30.0 64 24.5 65 19.0 66 17.0 67 15.0 68 9.5 69 5.0 70 5.5 71 7.0 72 5.5 73 5.0 74 4.0 75 2.0 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0125 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.125 40-41 0.08750000000000001 42-43 0.0375 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.13749999999999998 54-55 0.0 56-57 0.0125 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 18.0 71 23.0 72 16.0 73 23.0 74 22.0 75 22.0 76 16.0 77 13.0 78 17.0 79 20.0 80 15.0 81 21.0 82 17.0 83 16.0 84 22.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3719.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.75 #Duplication Level Percentage of deduplicated Percentage of total 1 93.67164179104478 78.45 2 3.8507462686567164 6.45 3 1.0149253731343284 2.55 4 0.44776119402985076 1.5 5 0.1492537313432836 0.625 6 0.208955223880597 1.05 7 0.1791044776119403 1.05 8 0.029850746268656716 0.2 9 0.029850746268656716 0.22499999999999998 >10 0.3880597014925373 5.575 >50 0.029850746268656716 2.325 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 93 2.325 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 31 0.775 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 23 0.575 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 22 0.5499999999999999 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 20 0.5 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 18 0.44999999999999996 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 17 0.42500000000000004 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 17 0.42500000000000004 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 16 0.4 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 12 0.3 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 12 0.3 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 12 0.3 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 12 0.3 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 11 0.27499999999999997 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 9 0.22499999999999998 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 8 0.2 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 7 0.17500000000000002 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 7 0.17500000000000002 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 7 0.17500000000000002 No Hit GTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCA 7 0.17500000000000002 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 7 0.17500000000000002 No Hit GGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTG 7 0.17500000000000002 No Hit GGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCCCCACGAGGCGCC 6 0.15 No Hit CAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATAT 6 0.15 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 6 0.15 No Hit GGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTCCGTCCAAGGC 6 0.15 No Hit GGGACGCATGCAACGACCATCTACATATAGCTACTCGATCTACCGCTACC 6 0.15 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 6 0.15 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 6 0.15 No Hit GGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGAG 5 0.125 No Hit GGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAG 5 0.125 No Hit GGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAGGCAAA 5 0.125 No Hit GGAAATTAACAGTTGGAAAGGGCGATCGGTCTTGATCCCTCTGTGTTTCC 5 0.125 No Hit CAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0125 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAGAG 20 0.0028448023 64.575 1 >>END_MODULE Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205038 READS because READLEN < 1 Read 205038 spots for ERR6133477.sra Written 205038 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra Rejected 205028 READS because READLEN < 1 Read 205028 spots for ERR6133477.sra Written 205028 spots for ERR6133477.sra SRR ids: ['ERR6133477.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3jftpzbq ERR6133477.sra spots: 4100570 blocks: [[1, 205028], [205029, 410056], [410057, 615084], [615085, 820112], [820113, 1025140], [1025141, 1230168], [1230169, 1435196], [1435197, 1640224], [1640225, 1845252], [1845253, 2050280], [2050281, 2255308], [2255309, 2460336], [2460337, 2665364], [2665365, 2870392], [2870393, 3075420], [3075421, 3280448], [3280449, 3485476], [3485477, 3690504], [3690505, 3895532], [3895533, 4100570]] ERR6133477 file size 901203 ERR6133477 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133477 ERR6133477_1.fastq Input file: ERR6133477_1.fastq trimmed: ERR6133477-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:55:15 2024 >> started Sat Dec 7 06:55:18 2024 >> done (2.212s) 4100570 reads processed; of these: 287 ( 0.01%) short reads filtered out after trimming by size control 35 ( 0.00%) empty reads filtered out after trimming by size control 4100248 (99.99%) reads available; of these: 69623 ( 1.70%) trimmed reads available after processing 4030625 (98.30%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 41 0.00% 19 78 0.00% 20 43 0.00% 21 32 0.00% 22 43 0.00% 23 19 0.00% 24 16 0.00% 25 9 0.00% 26 17 0.00% 27 26 0.00% 28 110 0.00% 29 142 0.00% 30 26 0.00% 31 23 0.00% 32 36 0.00% 33 44 0.00% 34 51 0.00% 35 170 0.00% 36 879 0.02% 37 43 0.00% 38 65 0.00% 39 144 0.00% 40 104 0.00% 41 63 0.00% 42 23 0.00% 43 20 0.00% 44 28 0.00% 45 27 0.00% 46 39 0.00% 47 17 0.00% 48 19 0.00% 49 16 0.00% 50 23 0.00% 51 65 0.00% 52 30 0.00% 53 10 0.00% 54 7 0.00% 55 14 0.00% 56 16 0.00% 57 21 0.00% 58 20 0.00% 59 15 0.00% 60 35 0.00% 61 22 0.00% 62 4 0.00% 63 2 0.00% 64 2 0.00% 65 12 0.00% 66 12 0.00% 67 27 0.00% 68 44 0.00% 69 207 0.01% 70 20216 0.49% 71 20149 0.49% 72 21464 0.52% 73 19846 0.48% 74 19554 0.48% 75 20036 0.49% 76 17890 0.44% 77 18849 0.46% 78 20386 0.50% 79 22077 0.54% 80 20178 0.49% 81 21863 0.53% 82 24022 0.59% 83 25571 0.62% 84 20501 0.50% 85 151 0.00% 86 296 0.01% 87 410 0.01% 88 745 0.02% 89 1339 0.03% 90 3027 0.07% 91 9373 0.23% 92 46258 1.13% 93 3723046 90.80% 4100248 reads passed initial QC criterion=sequence-density sequence-density=0.64 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=33 prefix-density=0.64 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.03 sequence-density-rank=21 fanout-score=137.24 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=20.6 sequence=TTTTTTTTTAAGAATCCTCCATTTTTGTTCTTCCACCCATGCAATAGAGAGCAAATGGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGAGTCATGGAATAATGGTGAATTCAAATGTTTAGTTCTTTAGTATAAGAAGTATAAGAA Started job on | Dec 07 06:55:35 Started mapping on | Dec 07 06:55:35 Finished on | Dec 07 06:55:41 Mapping speed, Million of reads per hour | 2460.15 Number of input reads | 4100248 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 3076387 Uniquely mapped reads % | 75.03% Average mapped length | 91.24 Number of splices: Total | 110772 Number of splices: Annotated (sjdb) | 92190 Number of splices: GT/AG | 105679 Number of splices: GC/AG | 2389 Number of splices: AT/AC | 56 Number of splices: Non-canonical | 2648 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.03% Deletion average length | 1.67 Insertion rate per base | 0.02% Insertion average length | 1.84 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 889244 % of reads mapped to multiple loci | 21.69% Number of reads mapped to too many loci | 47782 % of reads mapped to too many loci | 1.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.05% % of reads unmapped: other | 0.07% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 134617 134617 134617 N_multimapping 889244 889244 889244 N_noFeature 189599 218477 2940425 N_ambiguous 120272 13236 359 UnstrandedReadsAssigned:2766516 PositiveStrandReadsAssigned:2844674 NegativeStrandReadsAssigned:135603 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133477 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133477-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 4,100,248 reads, 3,375,294 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,069 rounds 52973 ERR6133477.ke.tsv 35125 ERR6133477.se.tsv 88098 total ==> ERR6133477.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 98 28.4397 PNS24243 293 194 0 0 KQK14069 1603 1504 21 5.55936 KQK14071 474 375 0 0 ==> ERR6133477.se.tsv <== BRADI_1g14170v3 20 BRADI_1g53295v3 45 BRADI_1g59795v3 32 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 45 BRADI_1g74790v3 44 BRADI_1g09890v3 0 BRADI_1g77505v3 102 BRADI_1g48960v3 0 ERR6133477 completed mapping pipeline successfully