Starting /dee2/code/volunteer_pipeline.sh ERR6133478 current disk space = 1544882737152 free memory = 1598291572 ERR6133478 SRAfilesize 90e34d8231fdc5914a15c9427b71b74c ERR6133478.sra ERR6133478.sra file validated ERR6133478 is single end ERR6133478 is conventional basespace ERR6133478 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133478_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.441 37.0 33.0 37.0 33.0 37.0 2 36.45275 37.0 37.0 37.0 37.0 37.0 3 35.73525 37.0 37.0 37.0 33.0 37.0 4 35.08975 37.0 37.0 37.0 33.0 37.0 5 35.15275 37.0 37.0 37.0 33.0 37.0 6 35.50625 37.0 37.0 37.0 33.0 37.0 7 37.2035 37.0 37.0 40.0 33.0 40.0 8 37.25575 37.0 37.0 40.0 33.0 40.0 9 37.32975 37.0 37.0 40.0 33.0 40.0 10-11 37.300625 37.0 37.0 40.0 33.0 40.0 12-13 37.196375 37.0 37.0 40.0 33.0 40.0 14-15 37.194625 37.0 37.0 40.0 33.0 40.0 16-17 37.04675 37.0 37.0 40.0 33.0 40.0 18-19 36.87325 37.0 37.0 40.0 33.0 40.0 20-21 36.542500000000004 37.0 37.0 40.0 33.0 40.0 22-23 36.5775 37.0 37.0 40.0 33.0 40.0 24-25 36.486125 37.0 37.0 40.0 33.0 40.0 26-27 36.733000000000004 37.0 37.0 40.0 33.0 40.0 28-29 36.692125000000004 37.0 37.0 40.0 33.0 40.0 30-31 36.612875 37.0 37.0 40.0 33.0 40.0 32-33 36.530125 37.0 37.0 40.0 33.0 40.0 34-35 36.433125000000004 37.0 37.0 40.0 33.0 40.0 36-37 36.231125 37.0 37.0 40.0 33.0 40.0 38-39 36.241875 37.0 37.0 40.0 33.0 40.0 40-41 35.958375000000004 37.0 37.0 40.0 33.0 40.0 42-43 35.97425 37.0 35.0 40.0 33.0 40.0 44-45 36.03725 37.0 37.0 40.0 33.0 40.0 46-47 35.8605 37.0 35.0 37.0 33.0 40.0 48-49 35.802875 37.0 35.0 37.0 33.0 40.0 50-51 35.625375 37.0 33.0 37.0 33.0 40.0 52-53 35.3465 37.0 33.0 37.0 33.0 40.0 54-55 35.3565 37.0 33.0 37.0 33.0 40.0 56-57 35.145250000000004 37.0 33.0 37.0 33.0 38.5 58-59 34.179249999999996 37.0 33.0 37.0 27.0 37.0 60-61 34.751125 37.0 33.0 37.0 33.0 37.0 62-63 34.6295 37.0 33.0 37.0 33.0 37.0 64-65 34.536249999999995 37.0 33.0 37.0 30.0 37.0 66-67 34.485125 37.0 33.0 37.0 30.0 37.0 68-69 33.63475 35.0 33.0 37.0 27.0 37.0 70-71 33.85675094363362 35.0 33.0 37.0 27.0 37.0 72-73 34.176061752629195 37.0 33.0 37.0 27.0 37.0 74-75 34.168394460556286 37.0 33.0 37.0 27.0 37.0 76-77 34.109600408434595 37.0 33.0 37.0 27.0 37.0 78-79 33.9773474946125 37.0 33.0 37.0 27.0 37.0 80-81 33.84220180039063 37.0 33.0 37.0 27.0 37.0 82-83 33.69156401914374 37.0 33.0 37.0 27.0 37.0 84-85 33.38422723789304 37.0 33.0 37.0 27.0 37.0 86-87 33.39472976606615 37.0 33.0 37.0 27.0 37.0 88-89 33.50564667921484 37.0 33.0 37.0 27.0 37.0 90-91 33.21511158913687 33.0 33.0 37.0 27.0 37.0 92-93 33.23702608228018 35.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 14.0 21 12.0 22 14.0 23 21.0 24 24.0 25 23.0 26 40.0 27 38.0 28 51.0 29 68.0 30 83.0 31 116.0 32 132.0 33 196.0 34 303.0 35 539.0 36 858.0 37 910.0 38 544.0 39 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 88.825 2.725 2.4 6.05 2 72.32499999999999 15.725 7.175 4.775 3 35.725 38.625 14.924999999999999 10.725 4 33.975 27.35 19.625 19.05 5 25.0 29.9 26.025 19.075 6 19.75 38.175 24.6 17.474999999999998 7 36.525 28.325 20.05 15.1 8 32.074999999999996 29.925 22.425 15.575 9 26.924999999999997 28.349999999999998 27.650000000000002 17.075000000000003 10-11 27.6625 27.35 28.225 16.7625 12-13 28.15 25.924999999999997 28.525 17.4 14-15 22.175 29.2375 29.575000000000003 19.0125 16-17 24.5625 30.25 26.75 18.4375 18-19 24.05300662582823 27.103387923490434 29.278659832479057 19.564945618202277 20-21 25.29382345586397 25.03125781445361 29.119779944986245 20.555138784696176 22-23 26.950000000000003 24.175 28.962500000000002 19.9125 24-25 25.124999999999996 24.2625 30.2875 20.325 26-27 25.3 25.2125 29.2875 20.200000000000003 28-29 25.162499999999998 26.6625 27.825 20.349999999999998 30-31 26.275 24.962500000000002 27.8625 20.9 32-33 25.55 25.337500000000002 28.5625 20.549999999999997 34-35 25.5 26.400000000000002 27.712500000000002 20.3875 36-37 24.415551943992998 25.690711338917367 28.728591073884235 21.165145643205403 38-39 26.566604127579733 23.864915572232643 30.28142589118199 19.287054409005627 40-41 26.178861788617887 25.96622889305816 27.25453408380238 20.600375234521575 42-43 24.175 27.737499999999997 28.6375 19.45 44-45 24.2625 25.224999999999998 30.6875 19.825 46-47 24.837500000000002 25.087500000000002 28.599999999999998 21.475 48-49 24.1875 24.7375 30.362499999999997 20.7125 50-51 24.2375 27.3 29.362500000000004 19.1 52-53 24.846510462348075 25.961658939982456 28.755795013156245 20.436035584513217 54-55 23.8625 26.4625 29.9 19.775000000000002 56-57 25.174999999999997 26.4125 28.849999999999998 19.5625 58-59 24.3125 25.137500000000003 30.15 20.4 60-61 25.137500000000003 24.375 30.375000000000004 20.1125 62-63 23.25 27.5125 31.387500000000003 17.849999999999998 64-65 22.5875 28.1625 30.0875 19.162499999999998 66-67 23.625 27.05 29.8375 19.4875 68-69 22.6125 27.1 30.412499999999998 19.875 70-71 23.97792826686732 25.445196889892152 29.67143215450213 20.9054426887384 72-73 24.759249873289406 25.874303091738472 29.53623922959959 19.83020780537253 74-75 24.107942192096175 27.22854584985292 29.31321140810845 19.35030054994245 76-77 22.736652050554554 26.07686355429456 30.33273149342275 20.85375290172814 78-79 23.430580880437617 24.82417296170878 31.414430841364936 20.330815316488668 80-81 23.867664434816856 27.37298148877511 30.340028882762244 18.41932519364579 82-83 24.038206420801274 25.961793579198726 30.087556381002916 19.91244361899708 84-85 22.92728736248994 25.99946337536893 31.499865843842233 19.5733834182989 86-87 21.403603119118042 27.03683785963969 32.58940575423501 18.97015326700726 88-89 21.013713363807476 28.47539661199247 31.46006991126647 19.050820112933582 90-91 24.025275611723583 27.440172089271307 29.604732454961013 18.9298198440441 92-93 21.941382091960204 29.64506587792417 29.25517612261361 19.158375907502016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 4.5 18 4.0 19 0.0 20 0.0 21 1.5 22 2.0 23 5.5 24 5.5 25 5.0 26 13.5 27 15.5 28 19.0 29 26.0 30 33.0 31 41.0 32 50.5 33 74.5 34 98.0 35 114.5 36 141.0 37 167.5 38 189.0 39 189.0 40 190.5 41 209.0 42 225.0 43 235.0 44 213.5 45 215.5 46 210.5 47 174.0 48 147.0 49 142.0 50 131.0 51 112.0 52 117.0 53 116.0 54 120.5 55 96.5 56 67.0 57 66.5 58 66.0 59 50.5 60 36.0 61 33.5 62 30.5 63 31.5 64 25.0 65 22.5 66 24.0 67 20.5 68 18.0 69 11.5 70 5.0 71 3.0 72 3.0 73 3.0 74 3.0 75 2.5 76 1.0 77 0.0 78 0.5 79 1.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0125 20-21 0.025 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0125 38-39 0.0625 40-41 0.0625 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.2375 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 26.0 71 19.0 72 18.0 73 20.0 74 15.0 75 16.0 76 18.0 77 20.0 78 18.0 79 15.0 80 13.0 81 19.0 82 28.0 83 20.0 84 16.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3719.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.4 #Duplication Level Percentage of deduplicated Percentage of total 1 93.40277777777779 80.7 2 4.484953703703703 7.75 3 0.8680555555555556 2.25 4 0.3761574074074074 1.3 5 0.1736111111111111 0.75 6 0.11574074074074073 0.6 7 0.11574074074074073 0.7000000000000001 8 0.057870370370370364 0.4 9 0.08680555555555555 0.675 >10 0.31828703703703703 4.875 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 40 1.0 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 22 0.5499999999999999 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 22 0.5499999999999999 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 18 0.44999999999999996 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 17 0.42500000000000004 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 14 0.35000000000000003 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 14 0.35000000000000003 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 12 0.3 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 12 0.3 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 12 0.3 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 12 0.3 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 9 0.22499999999999998 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 9 0.22499999999999998 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 9 0.22499999999999998 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 8 0.2 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 8 0.2 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 7 0.17500000000000002 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 7 0.17500000000000002 No Hit GGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTCCT 7 0.17500000000000002 No Hit GGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTG 7 0.17500000000000002 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 6 0.15 No Hit GCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCT 6 0.15 No Hit GGGCTCGAGGAGCATATGTACATTTGAACCCTGACTACACATATACACAC 6 0.15 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 6 0.15 No Hit GGAAAAAATAAGAGATGTACGGTTGGTTCAAATAATTCCTGGGGTTCAAA 5 0.125 No Hit GGGATGGAGATGGCCGCGGAGAACGGCGGCTGCGGCTGCAGCACCTGCAA 5 0.125 No Hit GGATTTTATGCCTGTCATACCTCTATTCTTTTTTCTATTAGCCTTTGTTT 5 0.125 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 5 0.125 No Hit GTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATAGATCCGG 5 0.125 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.0875 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGAAG 20 0.0029709986 63.87188 1 >>END_MODULE Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104631 READS because READLEN < 1 Read 104631 spots for ERR6133478.sra Written 104631 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra Rejected 104623 READS because READLEN < 1 Read 104623 spots for ERR6133478.sra Written 104623 spots for ERR6133478.sra SRR ids: ['ERR6133478.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_fdnfs265 ERR6133478.sra spots: 2092468 blocks: [[1, 104623], [104624, 209246], [209247, 313869], [313870, 418492], [418493, 523115], [523116, 627738], [627739, 732361], [732362, 836984], [836985, 941607], [941608, 1046230], [1046231, 1150853], [1150854, 1255476], [1255477, 1360099], [1360100, 1464722], [1464723, 1569345], [1569346, 1673968], [1673969, 1778591], [1778592, 1883214], [1883215, 1987837], [1987838, 2092468]] ERR6133478 file size 459087 ERR6133478 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133478 ERR6133478_1.fastq Input file: ERR6133478_1.fastq trimmed: ERR6133478-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 06:56:41 2024 >> started Sat Dec 7 06:56:42 2024 >> done (1.220s) 2092468 reads processed; of these: 314 ( 0.02%) short reads filtered out after trimming by size control 42 ( 0.00%) empty reads filtered out after trimming by size control 2092112 (99.98%) reads available; of these: 35323 ( 1.69%) trimmed reads available after processing 2056789 (98.31%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 33 0.00% 19 49 0.00% 20 43 0.00% 21 20 0.00% 22 25 0.00% 23 12 0.00% 24 20 0.00% 25 9 0.00% 26 14 0.00% 27 52 0.00% 28 311 0.01% 29 99 0.00% 30 23 0.00% 31 21 0.00% 32 63 0.00% 33 68 0.00% 34 18 0.00% 35 55 0.00% 36 481 0.02% 37 8 0.00% 38 25 0.00% 39 54 0.00% 40 44 0.00% 41 16 0.00% 42 14 0.00% 43 51 0.00% 44 11 0.00% 45 19 0.00% 46 12 0.00% 47 10 0.00% 48 8 0.00% 49 9 0.00% 50 10 0.00% 51 8 0.00% 52 12 0.00% 53 4 0.00% 54 6 0.00% 55 1 0.00% 56 6 0.00% 57 11 0.00% 58 14 0.00% 59 4 0.00% 60 13 0.00% 61 9 0.00% 62 4 0.00% 63 4 0.00% 64 1 0.00% 65 6 0.00% 66 5 0.00% 67 11 0.00% 68 26 0.00% 69 72 0.00% 70 10236 0.49% 71 9081 0.43% 72 9757 0.47% 73 9163 0.44% 74 9610 0.46% 75 9670 0.46% 76 8763 0.42% 77 9337 0.45% 78 9822 0.47% 79 10732 0.51% 80 9545 0.46% 81 10770 0.51% 82 12003 0.57% 83 12037 0.58% 84 10532 0.50% 85 90 0.00% 86 134 0.01% 87 205 0.01% 88 373 0.02% 89 787 0.04% 90 1559 0.07% 91 4686 0.22% 92 23168 1.11% 93 1908128 91.21% 2092112 reads passed initial QC criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=33 prefix-density=0.57 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.04 sequence-density-rank=20 fanout-score=142.44 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=19.9 sequence=TTTTTTTTTTAAGAATCCTCCATTTTTGTTCTTCCACCCATGCAATAGAGAGCAAATGGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGAGTCATGGAATAATGGTGAATTCAAATGTTTAGTTCTTTAGTATAAGAAGTATAAGAA Started job on | Dec 07 06:57:03 Started mapping on | Dec 07 06:57:03 Finished on | Dec 07 06:57:08 Mapping speed, Million of reads per hour | 1506.32 Number of input reads | 2092112 Average input read length | 91 UNIQUE READS: Uniquely mapped reads number | 1641562 Uniquely mapped reads % | 78.46% Average mapped length | 91.20 Number of splices: Total | 57904 Number of splices: Annotated (sjdb) | 46774 Number of splices: GT/AG | 53997 Number of splices: GC/AG | 1880 Number of splices: AT/AC | 28 Number of splices: Non-canonical | 1999 Mismatch rate per base, % | 0.54% Deletion rate per base | 0.05% Deletion average length | 1.87 Insertion rate per base | 0.02% Insertion average length | 1.78 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 379291 % of reads mapped to multiple loci | 18.13% Number of reads mapped to too many loci | 17029 % of reads mapped to too many loci | 0.81% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.53% % of reads unmapped: other | 0.06% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 71259 71259 71259 N_multimapping 379291 379291 379291 N_noFeature 102872 120662 1565055 N_ambiguous 64994 6271 240 UnstrandedReadsAssigned:1473696 PositiveStrandReadsAssigned:1514629 NegativeStrandReadsAssigned:76267 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133478 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133478-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 2,092,112 reads, 1,731,045 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 937 rounds 52973 ERR6133478.ke.tsv 35125 ERR6133478.se.tsv 88098 total ==> ERR6133478.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 43 25.0143 PNS24243 293 194 0 0 KQK14069 1603 1504 31 16.4509 KQK14071 474 375 0 0 ==> ERR6133478.se.tsv <== BRADI_1g14170v3 31 BRADI_1g53295v3 27 BRADI_1g59795v3 16 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 23 BRADI_1g74790v3 21 BRADI_1g09890v3 0 BRADI_1g77505v3 30 BRADI_1g48960v3 1 ERR6133478 completed mapping pipeline successfully