Starting /dee2/code/volunteer_pipeline.sh ERR6133482 current disk space = 1544858243072 free memory = 1604255096 ERR6133482 SRAfilesize c7ee0e22a5d27e21711da186959eb107 ERR6133482.sra ERR6133482.sra file validated ERR6133482 is single end ERR6133482 is conventional basespace ERR6133482 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133482_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.5325 37.0 33.0 37.0 33.0 37.0 2 36.405 37.0 37.0 37.0 37.0 37.0 3 35.7575 37.0 37.0 37.0 33.0 37.0 4 35.193 37.0 37.0 37.0 33.0 37.0 5 35.13575 37.0 37.0 37.0 33.0 37.0 6 35.42275 37.0 37.0 37.0 33.0 37.0 7 37.18575 37.0 37.0 40.0 33.0 40.0 8 37.2575 37.0 37.0 40.0 33.0 40.0 9 37.376 37.0 37.0 40.0 33.0 40.0 10-11 37.251374999999996 37.0 37.0 40.0 33.0 40.0 12-13 37.195750000000004 37.0 37.0 40.0 33.0 40.0 14-15 37.198625 37.0 37.0 40.0 33.0 40.0 16-17 37.13875 37.0 37.0 40.0 33.0 40.0 18-19 36.917 37.0 37.0 40.0 33.0 40.0 20-21 36.668875 37.0 37.0 40.0 33.0 40.0 22-23 36.647125 37.0 37.0 40.0 33.0 40.0 24-25 36.564625 37.0 37.0 40.0 33.0 40.0 26-27 36.7285 37.0 37.0 40.0 33.0 40.0 28-29 36.733875 37.0 37.0 40.0 33.0 40.0 30-31 36.660375 37.0 37.0 40.0 33.0 40.0 32-33 36.494125 37.0 37.0 40.0 33.0 40.0 34-35 36.43375 37.0 37.0 40.0 33.0 40.0 36-37 36.44475 37.0 37.0 40.0 33.0 40.0 38-39 36.421 37.0 37.0 40.0 33.0 40.0 40-41 36.196625 37.0 37.0 40.0 33.0 40.0 42-43 36.129 37.0 37.0 40.0 33.0 40.0 44-45 36.117625000000004 37.0 35.0 40.0 33.0 40.0 46-47 36.063 37.0 37.0 40.0 33.0 40.0 48-49 35.957875 37.0 37.0 37.0 33.0 40.0 50-51 35.804874999999996 37.0 33.0 37.0 33.0 40.0 52-53 35.571875 37.0 33.0 37.0 33.0 40.0 54-55 35.512375000000006 37.0 33.0 37.0 33.0 40.0 56-57 35.301500000000004 37.0 33.0 37.0 33.0 40.0 58-59 34.231625 37.0 33.0 37.0 27.0 37.0 60-61 34.691375 37.0 33.0 37.0 30.0 37.0 62-63 34.663125 37.0 33.0 37.0 30.0 37.0 64-65 34.545874999999995 37.0 33.0 37.0 30.0 37.0 66-67 34.5405 37.0 33.0 37.0 33.0 37.0 68-69 33.7405 35.0 33.0 37.0 30.0 37.0 70-71 33.886277289400454 35.0 33.0 37.0 27.0 37.0 72-73 34.29197413091433 37.0 33.0 37.0 30.0 37.0 74-75 34.305097305068955 37.0 33.0 37.0 33.0 37.0 76-77 34.07726690252608 37.0 33.0 37.0 27.0 37.0 78-79 33.94084426194304 37.0 33.0 37.0 27.0 37.0 80-81 33.849094526787745 37.0 33.0 37.0 27.0 37.0 82-83 33.73303748019322 37.0 33.0 37.0 27.0 37.0 84-85 33.41175834744662 35.0 33.0 37.0 27.0 37.0 86-87 33.45760190419519 37.0 33.0 37.0 27.0 37.0 88-89 33.590895566795595 37.0 33.0 37.0 27.0 37.0 90-91 33.324010711097884 33.0 33.0 37.0 27.0 37.0 92-93 33.31285331746504 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 11.0 21 10.0 22 8.0 23 17.0 24 19.0 25 27.0 26 31.0 27 49.0 28 46.0 29 70.0 30 96.0 31 121.0 32 144.0 33 168.0 34 269.0 35 538.0 36 835.0 37 901.0 38 593.0 39 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 85.15 4.825 3.925 6.1 2 68.55 16.900000000000002 9.425 5.125 3 38.6 35.8 15.7 9.9 4 30.175 31.8 20.65 17.375 5 28.549999999999997 29.925 24.349999999999998 17.175 6 21.025 37.675 24.85 16.45 7 37.325 28.375 19.625 14.674999999999999 8 29.5 29.45 23.724999999999998 17.325 9 26.825 30.0 28.275 14.899999999999999 10-11 25.112499999999997 29.312500000000004 27.9125 17.6625 12-13 25.0625 28.275 28.6625 18.0 14-15 21.762500000000003 32.05 29.1625 17.025000000000002 16-17 24.875 30.5375 25.2875 19.3 18-19 24.4125 26.7625 30.1875 18.637500000000003 20-21 24.803100387548444 25.428178522315285 29.128641080135015 20.64008001000125 22-23 27.187499999999996 24.8 28.212500000000002 19.8 24-25 24.5625 24.4875 29.512500000000003 21.4375 26-27 26.0375 24.7875 28.537499999999998 20.6375 28-29 24.275 27.737499999999997 29.625 18.3625 30-31 29.8875 24.175 26.8625 19.075 32-33 26.5125 26.3625 26.625 20.5 34-35 24.2625 29.75 26.650000000000002 19.3375 36-37 25.4875 25.0625 26.8625 22.5875 38-39 28.48568213079905 24.70926597474053 29.14843066149806 17.65662123296236 40-41 24.5311327831958 25.018754688672168 30.745186296574147 19.70492623155789 42-43 24.90311288911114 30.12876609576197 26.840855106888363 18.12726590823853 44-45 24.6875 26.775 29.2375 19.3 46-47 26.137500000000003 24.6875 27.3625 21.8125 48-49 24.95 25.1 29.275000000000002 20.674999999999997 50-51 22.7375 29.7875 27.437499999999996 20.0375 52-53 22.436700927550763 28.002005515166704 26.873903233893202 22.68739032338932 54-55 22.440305038129765 28.9536192024003 29.5286910863858 19.077384673084136 56-57 25.209453545079402 29.310991621858197 27.147680380142553 18.331874452919845 58-59 21.9375 28.8625 30.625000000000004 18.575 60-61 28.050000000000004 26.6625 26.8125 18.475 62-63 21.3625 30.0375 30.5125 18.087500000000002 64-65 22.537499999999998 32.487500000000004 27.0625 17.9125 66-67 25.1 30.525000000000002 27.737499999999997 16.6375 68-69 22.725 27.1 29.1375 21.0375 70-71 23.77719099710801 26.845215641896143 27.10926694329184 22.268326417704014 72-73 26.167265264238072 25.64135454079015 31.0159055926116 17.175474602360186 74-75 24.851993158794897 29.3908696224181 26.746480726220234 19.010656492566767 76-77 22.301193509454205 26.833847391712485 27.705511599839078 23.159447498994233 78-79 24.815523367040175 27.739819622847772 28.819349549057122 18.625307461054934 80-81 22.375767727526522 33.65438302624232 26.549413735343386 17.42043551088777 82-83 24.73457675753228 26.800573888091822 27.50358680057389 20.96126255380201 84-85 22.902796271637815 26.527592839177395 29.94525817428614 20.624352714898652 86-87 22.07676286819399 29.02409997024695 27.982743231181196 20.916393930377865 88-89 19.860160666468314 29.767926212436773 30.42249330556382 19.94941981553109 90-91 25.022314787265692 31.121689973222256 25.72151145492413 18.13448378458792 92-93 20.380839036001188 32.80273728057126 27.5662005355549 19.25022314787266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 2.5 19 3.5 20 2.5 21 1.0 22 2.0 23 5.0 24 6.5 25 8.0 26 16.0 27 21.0 28 26.5 29 36.5 30 39.5 31 44.0 32 63.0 33 89.0 34 112.0 35 129.5 36 139.5 37 180.5 38 213.5 39 205.0 40 209.0 41 201.5 42 195.5 43 207.5 44 194.5 45 187.5 46 199.0 47 182.0 48 156.5 49 152.5 50 138.0 51 123.5 52 115.5 53 123.0 54 188.5 55 161.5 56 69.5 57 51.0 58 43.5 59 27.0 60 22.0 61 23.5 62 16.0 63 13.0 64 16.0 65 17.0 66 13.5 67 11.0 68 11.5 69 10.5 70 8.0 71 8.0 72 6.0 73 2.0 74 1.0 75 1.0 76 1.0 77 1.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0125 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0375 40-41 0.025 42-43 0.0125 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.27499999999999997 54-55 0.0125 56-57 0.0375 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 47.0 71 36.0 72 38.0 73 57.0 74 43.0 75 36.0 76 29.0 77 39.0 78 32.0 79 42.0 80 38.0 81 46.0 82 64.0 83 55.0 84 37.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3361.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.925 #Duplication Level Percentage of deduplicated Percentage of total 1 93.18110728808257 74.47500000000001 2 4.191429465123553 6.7 3 0.8758210822646231 2.1 4 0.5004691898654989 1.6 5 0.3440725680325305 1.375 6 0.06255864873318737 0.3 7 0.15639662183296843 0.8750000000000001 8 0.06255864873318737 0.4 9 0.06255864873318737 0.44999999999999996 >10 0.5317485142320926 7.6 >50 0.0 0.0 >100 0.03127932436659368 4.125 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 165 4.125 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 32 0.8 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 29 0.7250000000000001 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 27 0.675 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 23 0.575 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 22 0.5499999999999999 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 19 0.475 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 19 0.475 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 18 0.44999999999999996 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 17 0.42500000000000004 No Hit GGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTCCGTCCAAGGC 16 0.4 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 15 0.375 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 13 0.325 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 11 0.27499999999999997 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 11 0.27499999999999997 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 11 0.27499999999999997 No Hit GGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTCCT 11 0.27499999999999997 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 10 0.25 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 9 0.22499999999999998 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 9 0.22499999999999998 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 8 0.2 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 8 0.2 No Hit GGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAA 7 0.17500000000000002 No Hit GGGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCG 7 0.17500000000000002 No Hit GGAGGAGATCGAGTTGTTACTTGAGAGTTTGTAACCCTTTATCATGCCAT 7 0.17500000000000002 No Hit GGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTAATAG 7 0.17500000000000002 No Hit GGTTTCAGTATATTGAAATAGAAAGATAAAATAGAAGAGAGAGGATAGGC 7 0.17500000000000002 No Hit GGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGT 6 0.15 No Hit GTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTA 6 0.15 No Hit GGGGGAGAAGTCTTATGTTATATATGGTAATCGCCTTGCCTATAGTGCCC 5 0.125 No Hit GGGAATGCAGCCCAAATCGGGCGGTAGACTCCGTCCAAGGCTAAATACAG 5 0.125 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 5 0.125 No Hit GGATATCGTGTGTGTACATTTGAATGTACCGACATGGGCTCGAGGAGCAT 5 0.125 No Hit GGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTG 5 0.125 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 5 0.125 No Hit GGGGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCT 5 0.125 No Hit GGGCCGGGTATCTCTCTGCATGTACGTATGCCTGTAATGTACGTAGCTAC 5 0.125 No Hit GGAGATCGAGTTGTTACTTGAGAGTTTGTAACCCTTTATCATGCCATGTC 5 0.125 No Hit GTAGACACATGGATCCATACATCTCGATCGGAAAAGAATCAATAGAAGGA 5 0.125 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.037500000000000006 0.0 0.0 0.0 0.0 12-13 0.05 0.0 0.0 0.0 0.0 14-15 0.05 0.0 0.0 0.0 0.0 16-17 0.05 0.0 0.0 0.0 0.0 18-19 0.05 0.0 0.0 0.0 0.0 20-21 0.05 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.0625 0.0 0.0 0.0 0.0 32-33 0.075 0.0 0.0 0.0 0.0 34-35 0.075 0.0 0.0 0.0 0.0 36-37 0.0875 0.0 0.0 0.0 0.0 38-39 0.1 0.0 0.0 0.0 0.0 40-41 0.1125 0.0 0.0 0.0 0.0 42-43 0.125 0.0 0.0 0.0 0.0 44-45 0.125 0.0 0.0 0.0 0.0 46-47 0.125 0.0 0.0 0.0 0.0 48-49 0.15 0.0 0.0 0.0 0.0 50-51 0.15 0.0 0.0 0.0 0.0 52-53 0.15 0.0 0.0 0.0 0.0 54-55 0.15 0.0 0.0 0.0 0.0 56-57 0.15 0.0 0.0 0.0 0.0 58-59 0.15 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.15 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.16249999999999998 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42110 READS because READLEN < 1 Read 42110 spots for ERR6133482.sra Written 42110 spots for ERR6133482.sra Rejected 42128 READS because READLEN < 1 Read 42128 spots for ERR6133482.sra Written 42128 spots for ERR6133482.sra SRR ids: ['ERR6133482.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_84cuegub ERR6133482.sra spots: 842218 blocks: [[1, 42110], [42111, 84220], [84221, 126330], [126331, 168440], [168441, 210550], [210551, 252660], [252661, 294770], [294771, 336880], [336881, 378990], [378991, 421100], [421101, 463210], [463211, 505320], [505321, 547430], [547431, 589540], [589541, 631650], [631651, 673760], [673761, 715870], [715871, 757980], [757981, 800090], [800091, 842218]] ERR6133482 file size 181577 ERR6133482 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133482 ERR6133482_1.fastq Input file: ERR6133482_1.fastq trimmed: ERR6133482-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 07:01:01 2024 >> started Sat Dec 7 07:01:01 2024 >> done (0.618s) 842218 reads processed; of these: 262 ( 0.03%) short reads filtered out after trimming by size control 39 ( 0.00%) empty reads filtered out after trimming by size control 841917 (99.96%) reads available; of these: 13508 ( 1.60%) trimmed reads available after processing 828409 (98.40%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 38 0.00% 19 67 0.01% 20 45 0.01% 21 28 0.00% 22 43 0.01% 23 20 0.00% 24 21 0.00% 25 15 0.00% 26 19 0.00% 27 24 0.00% 28 169 0.02% 29 49 0.01% 30 29 0.00% 31 22 0.00% 32 39 0.00% 33 53 0.01% 34 25 0.00% 35 158 0.02% 36 447 0.05% 37 37 0.00% 38 45 0.01% 39 100 0.01% 40 60 0.01% 41 57 0.01% 42 15 0.00% 43 21 0.00% 44 20 0.00% 45 14 0.00% 46 10 0.00% 47 15 0.00% 48 14 0.00% 49 9 0.00% 50 11 0.00% 51 42 0.00% 52 16 0.00% 53 11 0.00% 54 9 0.00% 55 13 0.00% 56 16 0.00% 57 12 0.00% 58 20 0.00% 59 9 0.00% 60 13 0.00% 61 13 0.00% 62 1 0.00% 63 0 0.00% 64 1 0.00% 65 3 0.00% 66 4 0.00% 67 7 0.00% 68 22 0.00% 69 60 0.01% 70 9227 1.10% 71 8843 1.05% 72 9305 1.11% 73 8515 1.01% 74 8583 1.02% 75 8614 1.02% 76 7278 0.86% 77 7523 0.89% 78 8316 0.99% 79 9334 1.11% 80 8101 0.96% 81 8846 1.05% 82 10174 1.21% 83 10396 1.23% 84 7999 0.95% 85 25 0.00% 86 32 0.00% 87 59 0.01% 88 123 0.01% 89 239 0.03% 90 469 0.06% 91 1446 0.17% 92 7285 0.87% 93 699174 83.05% 841917 reads passed initial QC criterion=sequence-density sequence-density=1.73 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=30 prefix-density=1.72 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=23.04 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=1.0 sequence=GGTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTTGAGGCGCAGCAGTTGACTGGACATCTAGGGGTAAAGCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGACCC Started job on | Dec 07 07:01:12 Started mapping on | Dec 07 07:01:12 Finished on | Dec 07 07:01:16 Mapping speed, Million of reads per hour | 757.73 Number of input reads | 841917 Average input read length | 90 UNIQUE READS: Uniquely mapped reads number | 547923 Uniquely mapped reads % | 65.08% Average mapped length | 89.76 Number of splices: Total | 16282 Number of splices: Annotated (sjdb) | 13441 Number of splices: GT/AG | 15302 Number of splices: GC/AG | 396 Number of splices: AT/AC | 18 Number of splices: Non-canonical | 566 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.04% Deletion average length | 1.73 Insertion rate per base | 0.03% Insertion average length | 1.69 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 229303 % of reads mapped to multiple loci | 27.24% Number of reads mapped to too many loci | 16064 % of reads mapped to too many loci | 1.91% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.65% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 64691 64691 64691 N_multimapping 229303 229303 229303 N_noFeature 38646 44489 521826 N_ambiguous 22829 2556 118 UnstrandedReadsAssigned:486448 PositiveStrandReadsAssigned:500878 NegativeStrandReadsAssigned:25979 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133482 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133482-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 841,917 reads, 620,863 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 930 rounds 52973 ERR6133482.ke.tsv 35125 ERR6133482.se.tsv 88098 total ==> ERR6133482.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 21 34.4773 PNS24243 293 194 0 0 KQK14069 1603 1504 5 7.48843 KQK14071 474 375 0 0 ==> ERR6133482.se.tsv <== BRADI_1g14170v3 5 BRADI_1g53295v3 9 BRADI_1g59795v3 4 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 6 BRADI_1g74790v3 2 BRADI_1g09890v3 0 BRADI_1g77505v3 9 BRADI_1g48960v3 0 ERR6133482 completed mapping pipeline successfully