Starting /dee2/code/volunteer_pipeline.sh ERR6133496 current disk space = 1544584605696 free memory = 1447447208 ERR6133496 SRAfilesize 47ed60354aed56518e4ca0b7ed097225 ERR6133496.sra ERR6133496.sra file validated ERR6133496 is single end ERR6133496 is conventional basespace ERR6133496 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133496_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.41 37.0 33.0 37.0 33.0 37.0 2 36.4615 37.0 37.0 37.0 37.0 37.0 3 35.75125 37.0 37.0 37.0 33.0 37.0 4 35.1345 37.0 37.0 37.0 33.0 37.0 5 35.17825 37.0 37.0 37.0 33.0 37.0 6 35.53975 37.0 37.0 37.0 33.0 37.0 7 37.255 37.0 37.0 40.0 33.0 40.0 8 37.3375 37.0 37.0 40.0 33.0 40.0 9 37.38325 37.0 37.0 40.0 33.0 40.0 10-11 37.339875 37.0 37.0 40.0 33.0 40.0 12-13 37.293125 37.0 37.0 40.0 33.0 40.0 14-15 37.2705 37.0 37.0 40.0 33.0 40.0 16-17 37.178375 37.0 37.0 40.0 33.0 40.0 18-19 36.990750000000006 37.0 37.0 40.0 33.0 40.0 20-21 36.71787500000001 37.0 37.0 40.0 33.0 40.0 22-23 36.612625 37.0 37.0 40.0 33.0 40.0 24-25 36.52975 37.0 37.0 40.0 33.0 40.0 26-27 36.851124999999996 37.0 37.0 40.0 33.0 40.0 28-29 36.831125 37.0 37.0 40.0 33.0 40.0 30-31 36.764624999999995 37.0 37.0 40.0 33.0 40.0 32-33 36.644 37.0 37.0 40.0 33.0 40.0 34-35 36.634375 37.0 37.0 40.0 33.0 40.0 36-37 36.420874999999995 37.0 37.0 40.0 33.0 40.0 38-39 36.346625 37.0 37.0 40.0 33.0 40.0 40-41 36.104375 37.0 37.0 40.0 33.0 40.0 42-43 36.072625 37.0 37.0 40.0 33.0 40.0 44-45 36.108999999999995 37.0 37.0 40.0 33.0 40.0 46-47 35.969875 37.0 37.0 38.5 33.0 40.0 48-49 35.895250000000004 37.0 37.0 37.0 33.0 40.0 50-51 35.69925 37.0 35.0 37.0 33.0 40.0 52-53 35.579125000000005 37.0 33.0 37.0 33.0 40.0 54-55 35.377125 37.0 33.0 37.0 33.0 40.0 56-57 35.235375 37.0 33.0 37.0 33.0 40.0 58-59 34.278125 37.0 33.0 37.0 27.0 37.0 60-61 34.762625 37.0 33.0 37.0 33.0 37.0 62-63 34.64875 37.0 33.0 37.0 30.0 37.0 64-65 34.560375 37.0 33.0 37.0 30.0 37.0 66-67 34.529375 37.0 33.0 37.0 30.0 37.0 68-69 33.7605 35.0 33.0 37.0 30.0 37.0 70-71 33.9132859739283 35.0 33.0 37.0 27.0 37.0 72-73 34.34392741457097 37.0 33.0 37.0 30.0 37.0 74-75 34.26900180935273 37.0 33.0 37.0 27.0 37.0 76-77 34.169053957569915 37.0 33.0 37.0 27.0 37.0 78-79 34.15831223956688 37.0 33.0 37.0 27.0 37.0 80-81 33.93971087602762 37.0 33.0 37.0 27.0 37.0 82-83 33.78918324308468 37.0 33.0 37.0 27.0 37.0 84-85 33.642084162372555 37.0 33.0 37.0 27.0 37.0 86-87 33.71947109152191 37.0 33.0 37.0 27.0 37.0 88-89 33.783380865958 37.0 33.0 37.0 27.0 37.0 90-91 33.5828364013482 37.0 33.0 37.0 27.0 37.0 92-93 33.50324086077262 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 14.0 21 11.0 22 19.0 23 17.0 24 20.0 25 30.0 26 41.0 27 39.0 28 45.0 29 61.0 30 93.0 31 96.0 32 152.0 33 157.0 34 249.0 35 482.0 36 926.0 37 935.0 38 603.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 87.875 3.075 3.15 5.8999999999999995 2 71.525 16.25 7.3999999999999995 4.825 3 36.825 37.475 14.7 11.0 4 33.1 29.5 18.625 18.775 5 25.900000000000002 31.324999999999996 25.55 17.224999999999998 6 20.599999999999998 37.125 24.775 17.5 7 36.95 28.4 18.8 15.85 8 31.175000000000004 30.675 21.375 16.775000000000002 9 26.8 28.299999999999997 28.025 16.875 10-11 26.5375 27.437499999999996 27.750000000000004 18.275 12-13 28.449999999999996 25.474999999999998 27.85 18.224999999999998 14-15 21.975 29.7125 29.625 18.6875 16-17 25.0 31.087500000000002 25.974999999999998 17.9375 18-19 23.175 27.150000000000002 29.1375 20.5375 20-21 24.837500000000002 26.1625 28.549999999999997 20.45 22-23 26.974999999999998 24.0375 28.000000000000004 20.9875 24-25 25.3125 25.0125 29.299999999999997 20.375 26-27 26.437500000000004 24.1125 29.349999999999998 20.1 28-29 25.3125 27.5625 26.887499999999996 20.2375 30-31 27.250000000000004 25.35 26.937499999999996 20.4625 32-33 25.124999999999996 26.35 28.1125 20.4125 34-35 25.3125 26.887499999999996 27.762500000000003 20.0375 36-37 24.9875 24.1625 28.325 22.525000000000002 38-39 27.951475737868936 24.874937468734366 28.4392196098049 18.734367183591797 40-41 26.619154788697173 24.10602650662666 28.782195548887223 20.492623155788948 42-43 24.90311288911114 28.103512939117394 27.11588948618577 19.877484685585696 44-45 23.0875 26.3125 29.7375 20.8625 46-47 25.775 23.825 28.175 22.225 48-49 24.2375 25.087500000000002 29.075 21.6 50-51 24.625 26.6625 28.825 19.8875 52-53 24.484181568088033 26.42240840315118 27.58534450418907 21.508065524571716 54-55 24.65 26.4125 28.9125 20.025000000000002 56-57 25.124999999999996 26.174999999999997 29.862499999999997 18.8375 58-59 23.6125 26.200000000000003 29.525000000000002 20.6625 60-61 25.6 24.525 29.125 20.75 62-63 23.2125 26.174999999999997 31.0375 19.575 64-65 24.675 26.900000000000002 29.375 19.05 66-67 26.0375 27.325 27.962500000000002 18.675 68-69 23.3375 26.35 28.775000000000002 21.5375 70-71 24.521216672925274 26.311177869570663 28.21379396670422 20.95381149079985 72-73 25.93989689425374 25.436942034452407 29.37256381239784 19.25059725889601 74-75 23.7842617152962 26.487305797650624 30.42819249715801 19.30023998989516 76-77 22.85931751871115 25.941900291767094 29.024483064823038 22.174299124698717 78-79 24.688215831000253 25.362687706795622 29.54950369050649 20.399592771697634 80-81 23.08380173735309 28.69187531936638 30.05876341338784 18.165559529892693 82-83 24.688343400591183 25.806451612903224 29.494923531679735 20.010281454825858 84-85 23.3915857605178 24.880258899676374 30.834951456310677 20.893203883495147 86-87 22.115633912367123 26.004666839512574 31.345605392792326 20.534093855327974 88-89 21.27301011148561 27.663987555094632 31.164117189525538 19.89888514389422 90-91 24.9546279491833 26.74358309567021 29.232564169043297 19.069224786103188 92-93 23.061965257972517 27.27508426238009 29.608504018667357 20.054446460980035 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 7.5 18 9.5 19 3.0 20 1.0 21 0.0 22 0.5 23 2.5 24 4.0 25 5.5 26 10.0 27 12.5 28 18.5 29 26.0 30 25.5 31 23.5 32 38.0 33 69.0 34 78.5 35 105.5 36 137.0 37 142.5 38 164.5 39 173.0 40 188.0 41 202.5 42 207.0 43 216.0 44 199.0 45 186.5 46 215.0 47 202.0 48 161.5 49 158.0 50 157.0 51 154.0 52 136.5 53 124.5 54 139.5 55 110.5 56 63.5 57 58.5 58 58.0 59 47.5 60 35.0 61 32.5 62 33.0 63 33.0 64 31.0 65 24.0 66 20.0 67 19.0 68 15.0 69 13.0 70 10.0 71 8.0 72 8.5 73 8.5 74 4.0 75 2.0 76 2.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05 40-41 0.025 42-43 0.0125 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0375 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 11.0 71 9.0 72 7.0 73 9.0 74 11.0 75 6.0 76 11.0 77 3.0 78 8.0 79 7.0 80 8.0 81 14.0 82 11.0 83 17.0 84 11.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3857.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.725 #Duplication Level Percentage of deduplicated Percentage of total 1 94.40069991251093 80.925 2 3.6162146398366875 6.2 3 0.699912510936133 1.7999999999999998 4 0.23330417031204434 0.8 5 0.2916302128900554 1.25 6 0.2041411490230388 1.05 7 0.08748906386701663 0.525 8 0.08748906386701663 0.6 9 0.058326042578011085 0.44999999999999996 >10 0.2916302128900554 4.5 >50 0.029163021289005542 1.9 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 76 1.9 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 41 1.0250000000000001 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 23 0.575 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 17 0.42500000000000004 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 16 0.4 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 16 0.4 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 15 0.375 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 15 0.375 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 13 0.325 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 12 0.3 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 12 0.3 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 9 0.22499999999999998 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 9 0.22499999999999998 No Hit GGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACGA 8 0.2 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 8 0.2 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 8 0.2 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 7 0.17500000000000002 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 7 0.17500000000000002 No Hit GGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTG 7 0.17500000000000002 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 6 0.15 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 6 0.15 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 6 0.15 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 6 0.15 No Hit GGAGTGGGGGTGCCATACGCAAAAAGGAAGCGACTCATAGAATGGCAGAG 6 0.15 No Hit GTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTA 6 0.15 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 6 0.15 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 5 0.125 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 5 0.125 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 5 0.125 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 5 0.125 No Hit GTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA 5 0.125 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 5 0.125 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 5 0.125 No Hit CATTACTGATGGAGTGATGGTCCATAGAGCATTAGTTTCACTACCTTCGC 5 0.125 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 5 0.125 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.0625 0.0 0.0 0.0 0.0 44-45 0.075 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197978 READS because READLEN < 1 Read 197978 spots for ERR6133496.sra Written 197978 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra Rejected 197959 READS because READLEN < 1 Read 197959 spots for ERR6133496.sra Written 197959 spots for ERR6133496.sra SRR ids: ['ERR6133496.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__z0qq7zm ERR6133496.sra spots: 3959199 blocks: [[1, 197959], [197960, 395918], [395919, 593877], [593878, 791836], [791837, 989795], [989796, 1187754], [1187755, 1385713], [1385714, 1583672], [1583673, 1781631], [1781632, 1979590], [1979591, 2177549], [2177550, 2375508], [2375509, 2573467], [2573468, 2771426], [2771427, 2969385], [2969386, 3167344], [3167345, 3365303], [3365304, 3563262], [3563263, 3761221], [3761222, 3959199]] ERR6133496 file size 875539 ERR6133496 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133496 ERR6133496_1.fastq Input file: ERR6133496_1.fastq trimmed: ERR6133496-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 07:20:50 2024 >> started Sat Dec 7 07:20:52 2024 >> done (1.927s) 3959199 reads processed; of these: 209 ( 0.01%) short reads filtered out after trimming by size control 28 ( 0.00%) empty reads filtered out after trimming by size control 3958962 (99.99%) reads available; of these: 66378 ( 1.68%) trimmed reads available after processing 3892584 (98.32%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 33 0.00% 19 61 0.00% 20 20 0.00% 21 29 0.00% 22 41 0.00% 23 17 0.00% 24 15 0.00% 25 10 0.00% 26 8 0.00% 27 17 0.00% 28 22 0.00% 29 59 0.00% 30 12 0.00% 31 28 0.00% 32 20 0.00% 33 16 0.00% 34 19 0.00% 35 132 0.00% 36 537 0.01% 37 30 0.00% 38 34 0.00% 39 112 0.00% 40 78 0.00% 41 35 0.00% 42 12 0.00% 43 7 0.00% 44 13 0.00% 45 13 0.00% 46 7 0.00% 47 12 0.00% 48 8 0.00% 49 13 0.00% 50 7 0.00% 51 29 0.00% 52 10 0.00% 53 10 0.00% 54 8 0.00% 55 7 0.00% 56 3 0.00% 57 5 0.00% 58 6 0.00% 59 4 0.00% 60 11 0.00% 61 8 0.00% 62 2 0.00% 63 3 0.00% 64 2 0.00% 65 4 0.00% 66 11 0.00% 67 13 0.00% 68 22 0.00% 69 68 0.00% 70 7509 0.19% 71 7432 0.19% 72 7991 0.20% 73 7882 0.20% 74 7672 0.19% 75 7847 0.20% 76 7274 0.18% 77 7714 0.19% 78 8255 0.21% 79 9334 0.24% 80 8761 0.22% 81 9170 0.23% 82 9818 0.25% 83 10570 0.27% 84 8476 0.21% 85 151 0.00% 86 251 0.01% 87 418 0.01% 88 806 0.02% 89 1451 0.04% 90 3107 0.08% 91 9380 0.24% 92 46800 1.18% 93 3769190 95.21% 3958962 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=8.34 fanout-score-rank=25 prefix-density=0.54 prefix-fanout=5.1 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=24 fanout-score=279.26 fanout-score-rank=1 prefix-density=0.64 prefix-fanout=9.8 sequence=GAAGAAGAAAAGTTTTCTCAACATGGGGAGGAAGTCCCTCCGAAATTTGATTTGTTATTGTATTGTAAGGGGCTTTTTTAGTATTTATCTAAAGGAAGGAACAAACGAGGATAAGATAAAATTTCTTTAAATTTATTTTGCCCAAGTGGGATATATGGCATACTATTCTTTCCATTTTCATCTTTTTTTCTATTCCACTCCATCTAGATATAAGAAAGAACCCAATGCAATGAAATTCCACTAATATACAATACAAAAAAGAAGAA Started job on | Dec 07 07:21:09 Started mapping on | Dec 07 07:21:09 Finished on | Dec 07 07:21:15 Mapping speed, Million of reads per hour | 2375.38 Number of input reads | 3958962 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 3082774 Uniquely mapped reads % | 77.87% Average mapped length | 92.08 Number of splices: Total | 153123 Number of splices: Annotated (sjdb) | 128393 Number of splices: GT/AG | 147591 Number of splices: GC/AG | 3091 Number of splices: AT/AC | 51 Number of splices: Non-canonical | 2390 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.04% Deletion average length | 1.75 Insertion rate per base | 0.02% Insertion average length | 2.09 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 793187 % of reads mapped to multiple loci | 20.04% Number of reads mapped to too many loci | 26168 % of reads mapped to too many loci | 0.66% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.38% % of reads unmapped: other | 0.05% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 83001 83001 83001 N_multimapping 793187 793187 793187 N_noFeature 166842 197068 2941159 N_ambiguous 124399 13120 298 UnstrandedReadsAssigned:2791533 PositiveStrandReadsAssigned:2872586 NegativeStrandReadsAssigned:141317 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133496 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133496-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,958,962 reads, 3,357,824 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,028 rounds 52973 ERR6133496.ke.tsv 35125 ERR6133496.se.tsv 88098 total ==> ERR6133496.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 79 23.1374 PNS24243 293 194 0 0 KQK14069 1603 1504 91 24.3128 KQK14071 474 375 0 0 ==> ERR6133496.se.tsv <== BRADI_1g14170v3 90 BRADI_1g53295v3 95 BRADI_1g59795v3 21 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 57 BRADI_1g74790v3 64 BRADI_1g09890v3 0 BRADI_1g77505v3 86 BRADI_1g48960v3 0 ERR6133496 completed mapping pipeline successfully