Starting /dee2/code/volunteer_pipeline.sh ERR6133506 current disk space = 1544354181120 free memory = 1597823792 ERR6133506 SRAfilesize e66666b5b21d710136356de7006cf8b2 ERR6133506.sra ERR6133506.sra file validated ERR6133506 is single end ERR6133506 is conventional basespace ERR6133506 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133506_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61175 37.0 37.0 37.0 37.0 37.0 2 36.75975 37.0 37.0 37.0 37.0 37.0 3 36.507 37.0 37.0 37.0 37.0 37.0 4 35.874 37.0 37.0 37.0 33.0 37.0 5 35.94525 37.0 37.0 37.0 33.0 37.0 6 36.307 37.0 37.0 37.0 33.0 37.0 7 38.26225 40.0 37.0 40.0 37.0 40.0 8 38.3045 40.0 37.0 40.0 37.0 40.0 9 38.339 40.0 37.0 40.0 37.0 40.0 10-11 38.284875 40.0 37.0 40.0 37.0 40.0 12-13 38.204875 40.0 37.0 40.0 37.0 40.0 14-15 38.187 40.0 37.0 40.0 37.0 40.0 16-17 38.078500000000005 40.0 37.0 40.0 37.0 40.0 18-19 38.004000000000005 40.0 37.0 40.0 37.0 40.0 20-21 37.952 40.0 37.0 40.0 33.0 40.0 22-23 38.12675 40.0 37.0 40.0 37.0 40.0 24-25 38.072 40.0 37.0 40.0 37.0 40.0 26-27 37.968 40.0 37.0 40.0 35.0 40.0 28-29 37.8745 40.0 37.0 40.0 35.0 40.0 30-31 37.998875 40.0 37.0 40.0 37.0 40.0 32-33 38.024125 40.0 37.0 40.0 37.0 40.0 34-35 37.981750000000005 40.0 37.0 40.0 37.0 40.0 36-37 37.91925 37.0 37.0 40.0 37.0 40.0 38-39 37.812375 37.0 37.0 40.0 37.0 40.0 40-41 37.613125 37.0 37.0 40.0 37.0 40.0 42-43 37.589125 37.0 37.0 40.0 37.0 40.0 44-45 37.33925 37.0 37.0 40.0 35.0 40.0 46-47 37.253 37.0 37.0 40.0 33.0 40.0 48-49 37.123875 37.0 37.0 40.0 33.0 40.0 50-51 36.928125 37.0 37.0 37.0 33.0 40.0 52-53 36.5465 37.0 37.0 37.0 33.0 40.0 54-55 36.508125 37.0 37.0 37.0 33.0 40.0 56-57 36.381875 37.0 37.0 37.0 33.0 40.0 58-59 36.028125 37.0 37.0 37.0 33.0 37.0 60-61 36.12775 37.0 37.0 37.0 33.0 37.0 62-63 35.958875 37.0 37.0 37.0 33.0 37.0 64-65 35.893125 37.0 37.0 37.0 33.0 37.0 66-67 35.88225 37.0 37.0 37.0 33.0 37.0 68-69 34.97625 35.0 35.0 37.0 33.0 37.0 70-71 35.169421679197995 37.0 33.0 37.0 33.0 37.0 72-73 35.59406788350391 37.0 33.0 37.0 33.0 37.0 74-75 35.547373402598424 37.0 33.0 37.0 33.0 37.0 76-77 35.56472034707974 37.0 33.0 37.0 33.0 37.0 78-79 35.49637691303772 37.0 33.0 37.0 33.0 37.0 80-81 35.29887173287726 37.0 33.0 37.0 33.0 37.0 82-83 35.14887528086005 37.0 33.0 37.0 33.0 37.0 84-85 35.0242370750811 37.0 33.0 37.0 33.0 37.0 86-87 34.972866174920966 37.0 33.0 37.0 33.0 37.0 88-89 35.016069546891465 37.0 33.0 37.0 33.0 37.0 90-91 34.88448366701792 37.0 33.0 37.0 33.0 37.0 92-93 34.88211275026343 37.0 33.0 37.0 33.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 6.0 22 3.0 23 5.0 24 2.0 25 7.0 26 9.0 27 11.0 28 12.0 29 20.0 30 32.0 31 37.0 32 62.0 33 63.0 34 89.0 35 303.0 36 1142.0 37 1368.0 38 799.0 39 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 86.15 5.425 2.9499999999999997 5.475 2 72.95 15.625 8.200000000000001 3.225 3 36.975 38.324999999999996 13.5 11.200000000000001 4 34.050000000000004 28.375 18.425 19.15 5 25.35 31.55 25.924999999999997 17.175 6 21.425 39.35 24.025 15.2 7 35.675000000000004 29.875 19.75 14.7 8 31.374999999999996 30.599999999999998 23.05 14.975 9 27.450000000000003 27.925 27.700000000000003 16.925 10-11 26.8 26.8125 28.225 18.1625 12-13 27.575 26.9625 28.8625 16.6 14-15 23.2125 30.6875 28.725 17.375 16-17 23.65 31.924999999999997 26.4625 17.962500000000002 18-19 23.9125 27.6875 27.762500000000003 20.6375 20-21 24.587500000000002 26.674999999999997 28.575 20.1625 22-23 26.3625 23.9375 28.449999999999996 21.25 24-25 27.212500000000002 25.0 28.6375 19.15 26-27 25.5625 25.4375 31.374999999999996 17.625 28-29 26.150000000000002 27.375 27.2625 19.2125 30-31 27.525 27.400000000000002 26.400000000000002 18.675 32-33 24.175 28.15 27.8625 19.8125 34-35 25.55 26.8375 27.05 20.5625 36-37 24.3 24.2375 30.587500000000002 20.875 38-39 26.325 26.525 30.099999999999998 17.05 40-41 27.388694347173587 25.30015007503752 26.650825412706354 20.66033016508254 42-43 25.653206650831358 28.403550443805475 26.403300412551566 19.5399424928116 44-45 22.95 26.337500000000002 29.849999999999998 20.8625 46-47 23.6875 24.712500000000002 29.3875 22.2125 48-49 24.5375 25.8625 30.912499999999998 18.6875 50-51 25.15 26.85 29.125 18.875 52-53 25.457967377666247 29.121706398996235 26.70012547051443 18.72020075282309 54-55 23.48380642741028 30.198824559209704 28.323121170438913 17.994247842941103 56-57 26.125 25.924999999999997 29.037499999999998 18.912499999999998 58-59 24.175 25.3 28.512500000000003 22.0125 60-61 24.775 25.887500000000003 29.1875 20.150000000000002 62-63 21.9 28.075 31.662499999999998 18.3625 64-65 23.525 27.275 29.849999999999998 19.35 66-67 24.175 27.775 29.1125 18.9375 68-69 22.8 27.05 28.349999999999998 21.8 70-71 24.680851063829788 26.458072590738425 28.147684605757195 20.713391739674595 72-73 25.771896660365467 24.52425960932577 28.834278512917454 20.869565217391305 74-75 23.84459116302692 26.295073641442357 30.10411376333164 19.756221432199087 76-77 22.03346532124154 25.916464427129903 30.897943543236682 21.152126708391876 78-79 24.669150713092638 24.437877425157396 31.18334832326866 19.709623538481306 80-81 23.922887825074397 28.36071936861172 30.4955362918877 17.220856514426185 82-83 23.865414710485133 25.456442357850804 30.842462180490347 19.835680751173708 84-85 23.94162503286879 23.83644491191165 31.89587168025243 20.326058374967133 86-87 22.339304531085354 26.65964172813488 32.428872497365646 18.57218124341412 88-89 20.692834562697577 29.162276080084297 31.49367755532139 18.651211801896732 90-91 26.462065331928347 26.23814541622761 29.07007376185458 18.229715489989463 92-93 23.063751317175974 30.150158061116965 28.635405690200212 18.15068493150685 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.5 18 4.5 19 1.5 20 1.0 21 1.0 22 1.0 23 4.0 24 6.5 25 5.5 26 9.5 27 14.5 28 18.5 29 21.0 30 24.0 31 30.5 32 43.0 33 63.5 34 79.5 35 101.0 36 126.5 37 141.5 38 170.5 39 186.0 40 184.0 41 196.5 42 219.0 43 233.5 44 215.0 45 202.5 46 246.5 47 236.5 48 183.5 49 180.0 50 175.5 51 167.5 52 154.5 53 158.5 54 133.5 55 81.5 56 63.5 57 59.0 58 50.5 59 32.0 60 19.5 61 18.0 62 16.0 63 12.0 64 10.5 65 10.0 66 9.0 67 6.0 68 5.0 69 4.5 70 3.0 71 3.0 72 2.0 73 1.0 74 1.5 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.05 42-43 0.0125 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.375 54-55 0.0375 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 10.0 71 17.0 72 11.0 73 15.0 74 18.0 75 9.0 76 11.0 77 10.0 78 15.0 79 13.0 80 13.0 81 16.0 82 16.0 83 16.0 84 14.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3796.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.625 #Duplication Level Percentage of deduplicated Percentage of total 1 93.30232558139535 75.225 2 4.124031007751938 6.65 3 1.0852713178294573 2.625 4 0.31007751937984496 1.0 5 0.15503875968992248 0.625 6 0.15503875968992248 0.75 7 0.15503875968992248 0.8750000000000001 8 0.062015503875969 0.4 9 0.09302325581395349 0.675 >10 0.5271317829457364 9.675 >50 0.0310077519379845 1.5 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 60 1.5 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 43 1.075 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 36 0.8999999999999999 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 31 0.775 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 30 0.75 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 27 0.675 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 27 0.675 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 26 0.65 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 24 0.6 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 23 0.575 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 20 0.5 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 19 0.475 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 18 0.44999999999999996 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 16 0.4 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 13 0.325 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 13 0.325 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 11 0.27499999999999997 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 10 0.25 No Hit GGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTT 9 0.22499999999999998 No Hit GAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCG 9 0.22499999999999998 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 9 0.22499999999999998 No Hit GGGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACG 8 0.2 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 8 0.2 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 7 0.17500000000000002 No Hit GGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACGA 7 0.17500000000000002 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 7 0.17500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 7 0.17500000000000002 No Hit GGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTCCGTCCAAGGC 7 0.17500000000000002 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 6 0.15 No Hit GGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCAT 6 0.15 No Hit GGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAG 6 0.15 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 6 0.15 No Hit GGGCTTGGCTACAATTCCGAATACGCTATTACATGTTTGCGCTAGTTTTT 6 0.15 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 5 0.125 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 5 0.125 No Hit GGGCAAGGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAA 5 0.125 No Hit GTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCA 5 0.125 No Hit GGGTGTGAGCTGGAGAGACGATCGGGTCTCTCAGCCGGCGTCTTCATCAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0125 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.075 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79372 READS because READLEN < 1 Read 79372 spots for ERR6133506.sra Written 79372 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra Rejected 79356 READS because READLEN < 1 Read 79356 spots for ERR6133506.sra Written 79356 spots for ERR6133506.sra SRR ids: ['ERR6133506.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_acl_xaxe ERR6133506.sra spots: 1587136 blocks: [[1, 79356], [79357, 158712], [158713, 238068], [238069, 317424], [317425, 396780], [396781, 476136], [476137, 555492], [555493, 634848], [634849, 714204], [714205, 793560], [793561, 872916], [872917, 952272], [952273, 1031628], [1031629, 1110984], [1110985, 1190340], [1190341, 1269696], [1269697, 1349052], [1349053, 1428408], [1428409, 1507764], [1507765, 1587136]] ERR6133506 file size 348509 ERR6133506 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133506 ERR6133506_1.fastq Input file: ERR6133506_1.fastq trimmed: ERR6133506-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 07:31:37 2024 >> started Sat Dec 7 07:31:38 2024 >> done (1.155s) 1587136 reads processed; of these: 217 ( 0.01%) short reads filtered out after trimming by size control 8 ( 0.00%) empty reads filtered out after trimming by size control 1586911 (99.99%) reads available; of these: 7605 ( 0.48%) trimmed reads available after processing 1579306 (99.52%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 20 0.00% 19 66 0.00% 20 27 0.00% 21 23 0.00% 22 21 0.00% 23 15 0.00% 24 13 0.00% 25 8 0.00% 26 12 0.00% 27 11 0.00% 28 86 0.01% 29 93 0.01% 30 22 0.00% 31 23 0.00% 32 32 0.00% 33 23 0.00% 34 9 0.00% 35 67 0.00% 36 270 0.02% 37 19 0.00% 38 34 0.00% 39 81 0.01% 40 57 0.00% 41 48 0.00% 42 10 0.00% 43 11 0.00% 44 23 0.00% 45 8 0.00% 46 14 0.00% 47 10 0.00% 48 7 0.00% 49 4 0.00% 50 8 0.00% 51 38 0.00% 52 16 0.00% 53 4 0.00% 54 11 0.00% 55 6 0.00% 56 11 0.00% 57 26 0.00% 58 5 0.00% 59 6 0.00% 60 10 0.00% 61 7 0.00% 62 1 0.00% 63 1 0.00% 64 2 0.00% 65 1 0.00% 66 5 0.00% 67 2 0.00% 68 5 0.00% 69 38 0.00% 70 6542 0.41% 71 6135 0.39% 72 5895 0.37% 73 5846 0.37% 74 5671 0.36% 75 5790 0.36% 76 5104 0.32% 77 5070 0.32% 78 5604 0.35% 79 5436 0.34% 80 5198 0.33% 81 5532 0.35% 82 6206 0.39% 83 6563 0.41% 84 5273 0.33% 85 18 0.00% 86 31 0.00% 87 50 0.00% 88 93 0.01% 89 210 0.01% 90 400 0.03% 91 1097 0.07% 92 4032 0.25% 93 1493745 94.13% 1586911 reads passed initial QC criterion=sequence-density sequence-density=0.51 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=32 prefix-density=0.52 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=140.37 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=6.0 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTG Started job on | Dec 07 07:31:51 Started mapping on | Dec 07 07:31:51 Finished on | Dec 07 07:31:58 Mapping speed, Million of reads per hour | 816.13 Number of input reads | 1586911 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 1041285 Uniquely mapped reads % | 65.62% Average mapped length | 92.07 Number of splices: Total | 58993 Number of splices: Annotated (sjdb) | 48920 Number of splices: GT/AG | 56337 Number of splices: GC/AG | 1713 Number of splices: AT/AC | 34 Number of splices: Non-canonical | 909 Mismatch rate per base, % | 0.46% Deletion rate per base | 0.06% Deletion average length | 1.87 Insertion rate per base | 0.02% Insertion average length | 1.83 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 399638 % of reads mapped to multiple loci | 25.18% Number of reads mapped to too many loci | 14039 % of reads mapped to too many loci | 0.88% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.26% % of reads unmapped: other | 0.06% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 145988 145988 145988 N_multimapping 399638 399638 399638 N_noFeature 71959 81293 994965 N_ambiguous 42276 5311 117 UnstrandedReadsAssigned:927050 PositiveStrandReadsAssigned:954681 NegativeStrandReadsAssigned:46203 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133506 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133506-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,586,911 reads, 1,239,440 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 929 rounds 52973 ERR6133506.ke.tsv 35125 ERR6133506.se.tsv 88098 total ==> ERR6133506.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 45 34.8348 PNS24243 293 194 0 0 KQK14069 1603 1504 11 7.76784 KQK14071 474 375 0 0 ==> ERR6133506.se.tsv <== BRADI_1g14170v3 12 BRADI_1g53295v3 19 BRADI_1g59795v3 5 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 3 BRADI_1g74790v3 13 BRADI_1g09890v3 0 BRADI_1g77505v3 34 BRADI_1g48960v3 0 ERR6133506 completed mapping pipeline successfully