Starting /dee2/code/volunteer_pipeline.sh ERR6133519 current disk space = 1544472985600 free memory = 1451457344 ERR6133519 SRAfilesize 49865c8a12bd026267a8042144c61f1d ERR6133519.sra ERR6133519.sra file validated ERR6133519 is single end ERR6133519 is conventional basespace ERR6133519 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133519_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.631 37.0 37.0 37.0 37.0 37.0 2 36.7865 37.0 37.0 37.0 37.0 37.0 3 36.471 37.0 37.0 37.0 37.0 37.0 4 35.83075 37.0 37.0 37.0 33.0 37.0 5 35.95725 37.0 37.0 37.0 33.0 37.0 6 36.2755 37.0 37.0 37.0 33.0 37.0 7 38.27775 40.0 37.0 40.0 37.0 40.0 8 38.31925 40.0 37.0 40.0 37.0 40.0 9 38.29025 40.0 37.0 40.0 37.0 40.0 10-11 38.245125 40.0 37.0 40.0 37.0 40.0 12-13 38.203 40.0 37.0 40.0 37.0 40.0 14-15 38.217124999999996 40.0 37.0 40.0 37.0 40.0 16-17 38.076750000000004 40.0 37.0 40.0 37.0 40.0 18-19 38.012875 40.0 37.0 40.0 37.0 40.0 20-21 38.038624999999996 40.0 37.0 40.0 35.0 40.0 22-23 38.151250000000005 40.0 37.0 40.0 37.0 40.0 24-25 38.09525 40.0 37.0 40.0 37.0 40.0 26-27 38.002375 40.0 37.0 40.0 37.0 40.0 28-29 37.947375 40.0 37.0 40.0 37.0 40.0 30-31 37.99925 40.0 37.0 40.0 37.0 40.0 32-33 38.048625 40.0 37.0 40.0 37.0 40.0 34-35 37.95225 38.5 37.0 40.0 37.0 40.0 36-37 37.89775 37.0 37.0 40.0 37.0 40.0 38-39 37.8215 37.0 37.0 40.0 37.0 40.0 40-41 37.658249999999995 37.0 37.0 40.0 37.0 40.0 42-43 37.593374999999995 37.0 37.0 40.0 37.0 40.0 44-45 37.30525 37.0 37.0 40.0 35.0 40.0 46-47 37.226124999999996 37.0 37.0 40.0 33.0 40.0 48-49 37.034625 37.0 37.0 38.5 33.0 40.0 50-51 36.865125 37.0 37.0 37.0 33.0 40.0 52-53 36.447 37.0 37.0 37.0 33.0 40.0 54-55 36.439375 37.0 37.0 37.0 33.0 40.0 56-57 36.305125000000004 37.0 37.0 37.0 33.0 38.5 58-59 35.85875 37.0 37.0 37.0 33.0 37.0 60-61 36.003375 37.0 37.0 37.0 33.0 37.0 62-63 35.880875 37.0 37.0 37.0 33.0 37.0 64-65 35.7795 37.0 37.0 37.0 33.0 37.0 66-67 35.705125 37.0 37.0 37.0 33.0 37.0 68-69 34.907875000000004 35.0 35.0 37.0 33.0 37.0 70-71 35.092574536689206 37.0 33.0 37.0 33.0 37.0 72-73 35.58139273621197 37.0 33.0 37.0 33.0 37.0 74-75 35.516224368085915 37.0 33.0 37.0 33.0 37.0 76-77 35.48465358666269 37.0 33.0 37.0 33.0 37.0 78-79 35.44116719132768 37.0 33.0 37.0 33.0 37.0 80-81 35.32232760126047 37.0 33.0 37.0 33.0 37.0 82-83 35.18438714418188 37.0 33.0 37.0 33.0 37.0 84-85 35.03465047207729 37.0 33.0 37.0 33.0 37.0 86-87 35.03605337439056 37.0 33.0 37.0 33.0 37.0 88-89 35.040030792917634 37.0 33.0 37.0 33.0 37.0 90-91 34.92391583269182 37.0 33.0 37.0 33.0 37.0 92-93 34.91352322299204 37.0 33.0 37.0 33.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 3.0 21 5.0 22 7.0 23 6.0 24 7.0 25 6.0 26 5.0 27 11.0 28 13.0 29 14.0 30 35.0 31 38.0 32 50.0 33 58.0 34 122.0 35 317.0 36 1119.0 37 1370.0 38 804.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 89.64999999999999 2.225 2.65 5.475 2 75.075 15.275 6.5 3.15 3 37.0 38.5 13.100000000000001 11.4 4 33.475 29.4 18.625 18.5 5 25.5 31.4 25.624999999999996 17.474999999999998 6 20.474999999999998 39.6 24.5 15.425 7 35.875 28.7 20.175 15.25 8 29.625 29.4 24.825 16.150000000000002 9 26.25 28.925 27.325 17.5 10-11 24.887500000000003 27.462500000000002 28.9875 18.6625 12-13 26.987499999999997 27.1 27.6125 18.3 14-15 23.7 31.1 27.9375 17.2625 16-17 23.75 30.7875 25.874999999999996 19.5875 18-19 22.625 28.15 27.6125 21.6125 20-21 24.6 26.85 28.775000000000002 19.775000000000002 22-23 27.712500000000002 23.6125 27.6875 20.9875 24-25 26.674999999999997 24.625 28.1 20.599999999999998 26-27 25.874999999999996 25.087500000000002 30.55 18.4875 28-29 26.087500000000002 26.8 27.287499999999998 19.825 30-31 27.6375 26.687499999999996 26.087500000000002 19.5875 32-33 24.725 27.1375 27.55 20.5875 34-35 24.7375 26.474999999999998 28.65 20.1375 36-37 23.375 26.0 29.312500000000004 21.3125 38-39 25.775 26.1 30.1875 17.9375 40-41 27.140892611576444 25.715714464308036 26.728341042630326 20.415051881485187 42-43 25.128141017627204 29.466183272909113 26.60332541567696 18.802350293786724 44-45 23.9 25.924999999999997 29.175 21.0 46-47 24.775 23.8625 28.775000000000002 22.5875 48-49 25.0375 24.6 31.025000000000002 19.3375 50-51 25.974999999999998 27.287499999999998 27.5875 19.15 52-53 24.363636363636363 27.849529780564264 26.946708463949843 20.84012539184953 54-55 24.1375 29.375 27.762500000000003 18.725 56-57 26.35 25.5375 28.675 19.4375 58-59 24.637500000000003 24.087500000000002 29.275000000000002 22.0 60-61 24.6 25.087500000000002 29.799999999999997 20.5125 62-63 22.787499999999998 28.349999999999998 31.087500000000002 17.775 64-65 24.0 28.1375 28.599999999999998 19.2625 66-67 23.6875 27.6375 29.062500000000004 19.6125 68-69 22.6375 26.950000000000003 28.712500000000002 21.7 70-71 24.859251845364692 26.911047166270485 27.64919304391342 20.580507944451394 72-73 25.65004396432609 24.871247330737344 28.400954653937948 21.077754050998617 74-75 23.500504032258064 28.85584677419355 28.414818548387093 19.228830645161292 76-77 21.96539093090817 25.57787040545661 29.79664014146773 22.660098522167488 78-79 24.10782080485953 25.80359402682865 30.52391799544419 19.56466717286763 80-81 23.933468765870998 29.08836973082783 29.355002539360083 17.623158963941087 82-83 24.323634507401735 25.280755487493618 29.47932618683001 20.916283818274632 84-85 24.192721681189134 23.47514095335725 31.56073808303434 20.771399282419274 86-87 22.722607133692584 26.70002566076469 31.331793687451885 19.24557351809084 88-89 22.491660251475494 28.624583012573773 30.4593276879651 18.42442904798563 90-91 25.673595073133182 26.712856043110083 28.919681806517833 18.693867077238902 92-93 22.696946369001797 29.753656658968435 29.022324865280986 18.527072106748783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 4.5 18 5.5 19 1.0 20 0.0 21 0.5 22 1.5 23 6.0 24 8.0 25 5.0 26 4.5 27 8.0 28 13.5 29 18.5 30 19.5 31 24.5 32 35.0 33 48.0 34 55.5 35 66.5 36 103.5 37 135.0 38 168.5 39 187.5 40 200.0 41 220.5 42 220.0 43 217.0 44 212.0 45 213.0 46 244.0 47 232.5 48 194.0 49 181.5 50 171.0 51 169.0 52 168.5 53 172.5 54 157.5 55 108.0 56 55.5 57 37.0 58 41.0 59 38.0 60 24.5 61 16.5 62 15.0 63 16.0 64 14.5 65 10.5 66 6.5 67 3.5 68 2.0 69 3.0 70 3.0 71 1.0 72 0.5 73 0.5 74 1.0 75 1.5 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0125 42-43 0.0125 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.3125 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 7.0 71 7.0 72 11.0 73 5.0 74 4.0 75 6.0 76 3.0 77 4.0 78 4.0 79 7.0 80 8.0 81 13.0 82 6.0 83 8.0 84 10.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3897.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 92.87060301507537 73.925 2 4.5540201005025125 7.249999999999999 3 1.0050251256281406 2.4 4 0.4082914572864322 1.3 5 0.18844221105527637 0.75 6 0.18844221105527637 0.8999999999999999 7 0.06281407035175879 0.35000000000000003 8 0.09422110552763818 0.6 9 0.031407035175879394 0.22499999999999998 >10 0.5653266331658292 10.975 >50 0.031407035175879394 1.325 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 53 1.325 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 47 1.175 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 40 1.0 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 37 0.9249999999999999 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 33 0.8250000000000001 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 32 0.8 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 31 0.775 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 26 0.65 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 24 0.6 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 23 0.575 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 23 0.575 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 21 0.525 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 20 0.5 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 19 0.475 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 17 0.42500000000000004 No Hit GGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGGCCGATTAA 15 0.375 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 11 0.27499999999999997 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 10 0.25 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 10 0.25 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 9 0.22499999999999998 No Hit GGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACGA 8 0.2 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 8 0.2 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 8 0.2 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 7 0.17500000000000002 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 7 0.17500000000000002 No Hit GGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTA 6 0.15 No Hit GGGGATGGAGCGACAGAAGTATGGAAATAGGATAAGGTAGCGGCGAGACG 6 0.15 No Hit GGCGCGGCGTAAGCTATGACAATAAAAAGTGTGCTGTACCTGATGTGTCT 6 0.15 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 6 0.15 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 6 0.15 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 6 0.15 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 5 0.125 No Hit GGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAAA 5 0.125 No Hit GGATTTGAAGAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAG 5 0.125 No Hit GGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTGAAATCGGA 5 0.125 No Hit GGGTGTGAGCTGGAGAGACGATCGGGTCTCTCAGCCGGCGTCTTCATCAG 5 0.125 No Hit GCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.0625 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.125 0.0 0.0 0.0 0.0 52-53 0.125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.125 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGATG 15 8.7632105E-4 86.87499 1 >>END_MODULE Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42655 READS because READLEN < 1 Read 42655 spots for ERR6133519.sra Written 42655 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra Rejected 42641 READS because READLEN < 1 Read 42641 spots for ERR6133519.sra Written 42641 spots for ERR6133519.sra SRR ids: ['ERR6133519.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hto8d96n ERR6133519.sra spots: 852834 blocks: [[1, 42641], [42642, 85282], [85283, 127923], [127924, 170564], [170565, 213205], [213206, 255846], [255847, 298487], [298488, 341128], [341129, 383769], [383770, 426410], [426411, 469051], [469052, 511692], [511693, 554333], [554334, 596974], [596975, 639615], [639616, 682256], [682257, 724897], [724898, 767538], [767539, 810179], [810180, 852834]] ERR6133519 file size 187276 ERR6133519 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133519 ERR6133519_1.fastq Input file: ERR6133519_1.fastq trimmed: ERR6133519-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 07:47:55 2024 >> started Sat Dec 7 07:47:56 2024 >> done (0.778s) 852834 reads processed; of these: 152 ( 0.02%) short reads filtered out after trimming by size control 7 ( 0.00%) empty reads filtered out after trimming by size control 852675 (99.98%) reads available; of these: 4441 ( 0.52%) trimmed reads available after processing 848234 (99.48%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 46 0.01% 20 12 0.00% 21 21 0.00% 22 21 0.00% 23 5 0.00% 24 8 0.00% 25 4 0.00% 26 9 0.00% 27 8 0.00% 28 19 0.00% 29 62 0.01% 30 12 0.00% 31 17 0.00% 32 17 0.00% 33 15 0.00% 34 9 0.00% 35 129 0.02% 36 114 0.01% 37 19 0.00% 38 21 0.00% 39 43 0.01% 40 56 0.01% 41 32 0.00% 42 3 0.00% 43 11 0.00% 44 3 0.00% 45 8 0.00% 46 2 0.00% 47 9 0.00% 48 3 0.00% 49 5 0.00% 50 9 0.00% 51 33 0.00% 52 5 0.00% 53 1 0.00% 54 2 0.00% 55 2 0.00% 56 4 0.00% 57 10 0.00% 58 1 0.00% 59 4 0.00% 60 7 0.00% 61 7 0.00% 62 0 0.00% 63 0 0.00% 64 0 0.00% 65 0 0.00% 66 0 0.00% 67 2 0.00% 68 1 0.00% 69 12 0.00% 70 1686 0.20% 71 1563 0.18% 72 1640 0.19% 73 1475 0.17% 74 1520 0.18% 75 1451 0.17% 76 1277 0.15% 77 1402 0.16% 78 1482 0.17% 79 1598 0.19% 80 1482 0.17% 81 1699 0.20% 82 1813 0.21% 83 1688 0.20% 84 1529 0.18% 85 13 0.00% 86 22 0.00% 87 34 0.00% 88 77 0.01% 89 133 0.02% 90 245 0.03% 91 617 0.07% 92 2332 0.27% 93 825042 96.76% 852675 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=24 prefix-density=0.35 prefix-fanout=2.0 sequence=CAAGGCTAAATAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=57.64 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=5.7 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACT Started job on | Dec 07 07:48:12 Started mapping on | Dec 07 07:48:12 Finished on | Dec 07 07:48:15 Mapping speed, Million of reads per hour | 1023.21 Number of input reads | 852675 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 598592 Uniquely mapped reads % | 70.20% Average mapped length | 92.17 Number of splices: Total | 42322 Number of splices: Annotated (sjdb) | 36035 Number of splices: GT/AG | 41119 Number of splices: GC/AG | 779 Number of splices: AT/AC | 19 Number of splices: Non-canonical | 405 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.04% Deletion average length | 1.79 Insertion rate per base | 0.02% Insertion average length | 1.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 232333 % of reads mapped to multiple loci | 27.25% Number of reads mapped to too many loci | 5027 % of reads mapped to too many loci | 0.59% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.93% % of reads unmapped: other | 0.03% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 21750 21750 21750 N_multimapping 232333 232333 232333 N_noFeature 36168 41445 572818 N_ambiguous 23438 2941 62 UnstrandedReadsAssigned:538986 PositiveStrandReadsAssigned:554206 NegativeStrandReadsAssigned:25712 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133519 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133519-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 852,675 reads, 727,574 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 886 rounds 52973 ERR6133519.ke.tsv 35125 ERR6133519.se.tsv 88098 total ==> ERR6133519.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 25 32.7746 PNS24243 293 194 0 0 KQK14069 1603 1504 7 8.37147 KQK14071 474 375 0 0 ==> ERR6133519.se.tsv <== BRADI_1g14170v3 7 BRADI_1g53295v3 23 BRADI_1g59795v3 1 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 8 BRADI_1g74790v3 11 BRADI_1g09890v3 0 BRADI_1g77505v3 19 BRADI_1g48960v3 0 ERR6133519 completed mapping pipeline successfully