Starting /dee2/code/volunteer_pipeline.sh ERR6133526 current disk space = 1544429535232 free memory = 1600694816 ERR6133526 SRAfilesize 18ff398c79b48d3b6a67aaf83ef400e0 ERR6133526.sra ERR6133526.sra file validated ERR6133526 is single end ERR6133526 is conventional basespace ERR6133526 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133526_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.02175 37.0 33.0 37.0 33.0 37.0 2 36.2405 37.0 37.0 37.0 33.0 37.0 3 36.02 37.0 37.0 37.0 33.0 37.0 4 35.73575 37.0 37.0 37.0 33.0 37.0 5 35.67125 37.0 37.0 37.0 33.0 37.0 6 35.8215 37.0 37.0 37.0 33.0 37.0 7 37.70575 40.0 37.0 40.0 33.0 40.0 8 37.728 40.0 37.0 40.0 33.0 40.0 9 37.7785 40.0 37.0 40.0 33.0 40.0 10-11 37.70775 40.0 37.0 40.0 33.0 40.0 12-13 37.708124999999995 40.0 37.0 40.0 33.0 40.0 14-15 37.664375 40.0 37.0 40.0 33.0 40.0 16-17 37.52275 40.0 37.0 40.0 33.0 40.0 18-19 37.444875 40.0 37.0 40.0 33.0 40.0 20-21 37.301500000000004 40.0 37.0 40.0 33.0 40.0 22-23 37.2625 37.0 37.0 40.0 33.0 40.0 24-25 37.300375 38.5 37.0 40.0 33.0 40.0 26-27 37.24275 38.5 37.0 40.0 33.0 40.0 28-29 37.1775 38.5 37.0 40.0 33.0 40.0 30-31 37.062875 37.0 37.0 40.0 33.0 40.0 32-33 36.9475 37.0 37.0 40.0 33.0 40.0 34-35 36.852000000000004 37.0 37.0 40.0 33.0 40.0 36-37 36.756249999999994 37.0 37.0 40.0 33.0 40.0 38-39 36.580124999999995 37.0 37.0 40.0 33.0 40.0 40-41 36.287875 37.0 37.0 40.0 33.0 40.0 42-43 35.984875 37.0 37.0 40.0 33.0 40.0 44-45 35.778875 37.0 33.0 40.0 33.0 40.0 46-47 35.42274999999999 37.0 33.0 40.0 27.0 40.0 48-49 35.389624999999995 37.0 33.0 38.5 30.0 40.0 50-51 35.23125 37.0 33.0 37.0 27.0 40.0 52-53 35.073750000000004 37.0 33.0 37.0 27.0 40.0 54-55 34.85575 37.0 33.0 37.0 27.0 40.0 56-57 34.561499999999995 37.0 33.0 37.0 27.0 40.0 58-59 32.084374999999994 33.0 30.0 37.0 24.5 37.0 60-61 33.801874999999995 37.0 33.0 37.0 27.0 37.0 62-63 33.946 37.0 33.0 37.0 27.0 37.0 64-65 33.86825 37.0 33.0 37.0 27.0 37.0 66-67 33.531 37.0 33.0 37.0 27.0 37.0 68-69 32.907875000000004 35.0 33.0 37.0 27.0 37.0 70-71 33.08298903508772 35.0 33.0 37.0 27.0 37.0 72-73 33.391455962627276 37.0 33.0 37.0 27.0 37.0 74-75 33.3947504763093 37.0 33.0 37.0 27.0 37.0 76-77 33.40137380408104 37.0 33.0 37.0 27.0 37.0 78-79 33.40097375658512 37.0 33.0 37.0 27.0 37.0 80-81 33.270712098712124 37.0 33.0 37.0 27.0 37.0 82-83 32.98508930223042 35.0 33.0 37.0 27.0 37.0 84-85 32.80817557907627 33.0 33.0 37.0 27.0 37.0 86-87 32.889317889317894 33.0 33.0 37.0 27.0 37.0 88-89 33.04157014157014 35.0 33.0 37.0 27.0 37.0 90-91 32.60424710424711 33.0 33.0 37.0 27.0 37.0 92-93 32.7024453024453 33.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 19.0 21 21.0 22 22.0 23 25.0 24 31.0 25 33.0 26 45.0 27 53.0 28 65.0 29 72.0 30 111.0 31 123.0 32 146.0 33 202.0 34 265.0 35 384.0 36 752.0 37 949.0 38 670.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 79.14999999999999 6.25 6.875000000000001 7.725 2 56.599999999999994 23.175 13.850000000000001 6.375 3 28.199999999999996 38.2 17.8 15.8 4 29.925 26.35 20.424999999999997 23.3 5 23.95 28.925 30.725 16.400000000000002 6 17.299999999999997 41.349999999999994 24.075 17.275 7 33.4 28.175 22.35 16.075 8 28.9 27.325 25.424999999999997 18.35 9 23.1 28.15 26.525 22.225 10-11 23.1 28.6375 28.962500000000002 19.3 12-13 24.8125 24.75 28.975 21.462500000000002 14-15 22.1 29.6625 28.4125 19.825 16-17 24.6625 30.675 24.6625 20.0 18-19 24.025 26.387500000000003 28.787499999999998 20.8 20-21 24.56557069633704 25.015626953369168 28.60357544693087 21.81522690336292 22-23 27.325 22.8625 28.0625 21.75 24-25 24.375 26.25 29.1375 20.2375 26-27 25.7 24.5375 31.525 18.2375 28-29 25.224999999999998 27.037499999999998 27.400000000000002 20.3375 30-31 26.424999999999997 25.587500000000002 28.212500000000002 19.775000000000002 32-33 24.337500000000002 26.174999999999997 29.1625 20.325 34-35 23.87798474809351 27.91598949868734 26.140767595949495 22.06525815726966 36-37 23.74343585896474 26.944236059014752 27.481870467616904 21.8304576144036 38-39 26.072813711998 24.38383585637433 30.113849618416115 19.429500813211558 40-41 25.7125 26.224999999999998 27.8125 20.25 42-43 26.860072527197698 29.048393147430286 25.647117669125922 18.444416656246094 44-45 22.8875 27.0 29.325000000000003 20.7875 46-47 24.5 24.8625 28.95 21.6875 48-49 24.2625 24.587500000000002 28.575 22.575 50-51 22.011005502751377 27.363681840920464 28.83941970985493 21.785892946473236 52-53 24.72809101137642 27.00337542192774 25.640705088136016 22.62782847855982 54-55 25.2125 27.075 28.3875 19.325 56-57 26.075 26.775 26.9625 20.1875 58-59 23.799999999999997 24.6125 31.362499999999997 20.225 60-61 26.275 25.900000000000002 28.549999999999997 19.275000000000002 62-63 20.775 28.675 31.087500000000002 19.4625 64-65 21.3625 30.2125 29.037499999999998 19.3875 66-67 23.6375 29.2 28.325 18.8375 68-69 21.05 26.6625 28.425 23.8625 70-71 23.178973717146434 28.1351689612015 27.684605757196497 21.00125156445557 72-73 24.50672363956265 25.08483096644464 29.408068367475177 21.00037702651753 74-75 22.669357890753123 28.976914343383374 29.14091081115176 19.212816954711744 76-77 22.508851795649974 24.671219018715227 28.199291856348 24.620637329286797 78-79 26.51274895344412 25.637447672206015 27.679817328428264 20.169986045921604 80-81 23.11512990320937 29.737646459500766 30.20886398369842 16.93835965359144 82-83 24.015345268542198 25.345268542199488 30.26854219948849 20.37084398976982 84-85 22.680544847083013 23.001799023387303 33.71883834489849 20.5988177846312 86-87 21.38996138996139 27.25868725868726 30.630630630630627 20.72072072072072 88-89 20.823680823680824 29.755469755469754 29.53667953667954 19.884169884169882 90-91 24.517374517374517 27.31016731016731 29.472329472329474 18.7001287001287 92-93 21.814671814671815 29.45945945945946 29.74259974259974 18.98326898326898 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 8.0 18 12.5 19 5.0 20 1.5 21 6.0 22 8.0 23 11.0 24 12.5 25 9.0 26 14.5 27 20.5 28 30.0 29 39.5 30 32.5 31 35.5 32 52.5 33 70.0 34 79.5 35 81.5 36 126.0 37 182.5 38 206.0 39 182.0 40 167.5 41 178.5 42 195.0 43 215.5 44 189.5 45 167.0 46 179.5 47 169.0 48 134.0 49 144.5 50 177.0 51 181.5 52 161.0 53 151.5 54 158.5 55 114.0 56 59.5 57 51.0 58 47.5 59 38.5 60 33.5 61 36.0 62 31.0 63 21.5 64 21.5 65 20.0 66 14.0 67 12.0 68 10.5 69 6.5 70 6.0 71 7.0 72 5.5 73 3.0 74 2.5 75 1.5 76 1.0 77 2.0 78 1.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0125 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0125 36-37 0.025 38-39 0.08750000000000001 40-41 0.0 42-43 0.0375 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.05 52-53 0.0125 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 10.0 71 7.0 72 9.0 73 9.0 74 3.0 75 5.0 76 6.0 77 5.0 78 9.0 79 8.0 80 6.0 81 9.0 82 8.0 83 9.0 84 12.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3885.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.85 #Duplication Level Percentage of deduplicated Percentage of total 1 92.97956493078445 70.525 2 3.8562953197099534 5.8500000000000005 3 1.0217534607778511 2.325 4 0.3955174686882004 1.2 5 0.3955174686882004 1.5 6 0.2966381015161503 1.35 7 0.0988793671720501 0.525 8 0.16479894528675015 1.0 9 0.06591957811470006 0.44999999999999996 >10 0.6262359920896506 9.425 >50 0.0988793671720501 5.8500000000000005 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 94 2.35 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 82 2.0500000000000003 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 58 1.4500000000000002 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 41 1.0250000000000001 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 37 0.9249999999999999 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 32 0.8 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 31 0.775 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 24 0.6 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 22 0.5499999999999999 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 21 0.525 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 18 0.44999999999999996 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 18 0.44999999999999996 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 18 0.44999999999999996 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 16 0.4 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 14 0.35000000000000003 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 14 0.35000000000000003 No Hit TCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGA 13 0.325 No Hit GGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTC 12 0.3 No Hit GGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAGACTCCGTCCAAGGC 12 0.3 No Hit GGCTGCTTGGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTA 12 0.3 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 11 0.27499999999999997 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 11 0.27499999999999997 No Hit GGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGA 9 0.22499999999999998 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 9 0.22499999999999998 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 8 0.2 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 8 0.2 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 8 0.2 No Hit GGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTTG 8 0.2 No Hit GGCCGATTAATCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACA 8 0.2 No Hit GAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAGTTTTTCTTA 7 0.17500000000000002 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 7 0.17500000000000002 No Hit GTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAG 7 0.17500000000000002 No Hit AATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCC 6 0.15 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 6 0.15 No Hit GGGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCG 6 0.15 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 6 0.15 No Hit AACAGTAAAGCTTCATAGGGTCTTTCTGTCCAGGTGCAGGTAGTCCGCAT 6 0.15 No Hit AACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCG 6 0.15 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 6 0.15 No Hit GGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTT 6 0.15 No Hit AATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATTTCGTCGCTT 6 0.15 No Hit GGGAAAAGAGGGGTTACTTTTTTTCATTTTTCCCTTAAAAGATAGGCTTT 5 0.125 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTC 5 0.125 No Hit GAAGGAACAAACGAGGATAAGATAAAATTTCTTTAAATTTATTTTGCCCA 5 0.125 No Hit GGTCTTGATCCCTCTGTGTTTCCCGTGTAACGGCTACTGATCCAGTGGTT 5 0.125 No Hit GGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCTAAATACTCCT 5 0.125 No Hit CAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCT 5 0.125 No Hit GATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGC 5 0.125 No Hit GGTCAAGAGGAAGAGACTTATAATATTGTGGCTGCTCATGGTTATTTTGG 5 0.125 No Hit GTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAAT 5 0.125 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 5 0.125 No Hit GGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCA 5 0.125 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.09999999999999999 0.0 0.0 0.0 0.0 38-39 0.175 0.0 0.0 0.0 0.0 40-41 0.175 0.0 0.0 0.0 0.0 42-43 0.175 0.0 0.0 0.0 0.0 44-45 0.175 0.0 0.0 0.0 0.0 46-47 0.175 0.0 0.0 0.0 0.0 48-49 0.175 0.0 0.0 0.0 0.0 50-51 0.175 0.0 0.0 0.0 0.0 52-53 0.2 0.0 0.0 0.0 0.0 54-55 0.2 0.0 0.0 0.0 0.0 56-57 0.2 0.0 0.0 0.0 0.0 58-59 0.2 0.0 0.0 0.0 0.0 60-61 0.2 0.0 0.0 0.0 0.0 62-63 0.2 0.0 0.0 0.0 0.0 64-65 0.2 0.0 0.0 0.0 0.0 66-67 0.2 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313319 READS because READLEN < 1 Read 313319 spots for ERR6133526.sra Written 313319 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra Rejected 313316 READS because READLEN < 1 Read 313316 spots for ERR6133526.sra Written 313316 spots for ERR6133526.sra SRR ids: ['ERR6133526.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_5u01se38 ERR6133526.sra spots: 6266323 blocks: [[1, 313316], [313317, 626632], [626633, 939948], [939949, 1253264], [1253265, 1566580], [1566581, 1879896], [1879897, 2193212], [2193213, 2506528], [2506529, 2819844], [2819845, 3133160], [3133161, 3446476], [3446477, 3759792], [3759793, 4073108], [4073109, 4386424], [4386425, 4699740], [4699741, 5013056], [5013057, 5326372], [5326373, 5639688], [5639689, 5953004], [5953005, 6266323]] ERR6133526 file size 1386863 ERR6133526 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133526 ERR6133526_1.fastq Input file: ERR6133526_1.fastq trimmed: ERR6133526-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 07:56:35 2024 >> started Sat Dec 7 07:56:38 2024 >> done (2.976s) 6266323 reads processed; of these: 634 ( 0.01%) short reads filtered out after trimming by size control 186 ( 0.00%) empty reads filtered out after trimming by size control 6265503 (99.99%) reads available; of these: 136444 ( 2.18%) trimmed reads available after processing 6129059 (97.82%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 61 0.00% 19 168 0.00% 20 82 0.00% 21 94 0.00% 22 119 0.00% 23 32 0.00% 24 31 0.00% 25 35 0.00% 26 35 0.00% 27 50 0.00% 28 145 0.00% 29 124 0.00% 30 105 0.00% 31 180 0.00% 32 194 0.00% 33 205 0.00% 34 333 0.01% 35 1326 0.02% 36 13942 0.22% 37 280 0.00% 38 214 0.00% 39 485 0.01% 40 321 0.01% 41 265 0.00% 42 211 0.00% 43 228 0.00% 44 239 0.00% 45 213 0.00% 46 223 0.00% 47 221 0.00% 48 210 0.00% 49 179 0.00% 50 172 0.00% 51 213 0.00% 52 146 0.00% 53 115 0.00% 54 110 0.00% 55 101 0.00% 56 87 0.00% 57 97 0.00% 58 106 0.00% 59 63 0.00% 60 73 0.00% 61 48 0.00% 62 6 0.00% 63 14 0.00% 64 15 0.00% 65 17 0.00% 66 25 0.00% 67 31 0.00% 68 74 0.00% 69 197 0.00% 70 12422 0.20% 71 11887 0.19% 72 13614 0.22% 73 11669 0.19% 74 12205 0.19% 75 12368 0.20% 76 11301 0.18% 77 11849 0.19% 78 13472 0.22% 79 14179 0.23% 80 13881 0.22% 81 15881 0.25% 82 18260 0.29% 83 16149 0.26% 84 15785 0.25% 85 444 0.01% 86 716 0.01% 87 1250 0.02% 88 2188 0.03% 89 3655 0.06% 90 7388 0.12% 91 20288 0.32% 92 74915 1.20% 93 5927477 94.60% 6265503 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=10.64 fanout-score-rank=14 prefix-density=0.81 prefix-fanout=4.3 sequence=GAAGAAGAAGAAACAACTCCGGCCATGGCGGGCATCATCCACAAGATCGAGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=23.53 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=1.1 sequence=GGACATTTCTAATAATAGCAAGCCTTATTCCGATTTTGGCATTTTGGATTTCAGGGCTTTTAGCCCCGATTAGTGAAGGACCCGAAAAGCTTTCTAGTTATGAATCGGGTATAGAACCCATGGGAGGGGCTTGGCTACAATTCCGAATACGCTATTACATGTTTGCGCTAGTTTTTGTTGTTTTTGATGTGGAAACCGTCTTTCTCTACCCTTGGGCAATGAGTTTCGACGTATTGGGTGTATCCGTTTTTATCGAAGCTTTCATTTTCGTGCTTATCCTAGTTGTTGGTTTAGTTTATGCATGGCGAAAAGGAGCCTTGGAATGGTCTTAACTGAATATT Started job on | Dec 07 07:57:05 Started mapping on | Dec 07 07:57:05 Finished on | Dec 07 07:57:12 Mapping speed, Million of reads per hour | 3222.26 Number of input reads | 6265503 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 3764646 Uniquely mapped reads % | 60.09% Average mapped length | 92.00 Number of splices: Total | 220333 Number of splices: Annotated (sjdb) | 175967 Number of splices: GT/AG | 211489 Number of splices: GC/AG | 3828 Number of splices: AT/AC | 134 Number of splices: Non-canonical | 4882 Mismatch rate per base, % | 0.30% Deletion rate per base | 0.03% Deletion average length | 1.46 Insertion rate per base | 0.00% Insertion average length | 1.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 2265258 % of reads mapped to multiple loci | 36.15% Number of reads mapped to too many loci | 117819 % of reads mapped to too many loci | 1.88% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.72% % of reads unmapped: other | 0.16% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 235599 235599 235599 N_multimapping 2265258 2265258 2265258 N_noFeature 296413 337264 3605214 N_ambiguous 137452 18528 818 UnstrandedReadsAssigned:3330781 PositiveStrandReadsAssigned:3408854 NegativeStrandReadsAssigned:158614 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133526 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133526-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 6,265,503 reads, 4,876,737 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,044 rounds 52973 ERR6133526.ke.tsv 35125 ERR6133526.se.tsv 88098 total ==> ERR6133526.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 100 19.6813 PNS24243 293 194 0 0 KQK14069 1603 1504 370 66.4298 KQK14071 474 375 0 0 ==> ERR6133526.se.tsv <== BRADI_1g14170v3 370 BRADI_1g53295v3 21 BRADI_1g59795v3 33 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 46 BRADI_1g74790v3 43 BRADI_1g09890v3 1 BRADI_1g77505v3 120 BRADI_1g48960v3 0 ERR6133526 completed mapping pipeline successfully