Starting /dee2/code/volunteer_pipeline.sh ERR6133535 current disk space = 1544519954432 free memory = 1427429040 ERR6133535 SRAfilesize c78ecb3c387d5b102bad6b0b4b8a72d6 ERR6133535.sra ERR6133535.sra file validated ERR6133535 is single end ERR6133535 is conventional basespace ERR6133535 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133535_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.86325 33.0 33.0 37.0 27.0 37.0 2 35.9745 37.0 37.0 37.0 33.0 37.0 3 35.28075 37.0 33.0 37.0 33.0 37.0 4 35.30275 37.0 37.0 37.0 33.0 37.0 5 35.25675 37.0 37.0 37.0 33.0 37.0 6 35.62 37.0 37.0 37.0 33.0 37.0 7 37.44375 40.0 37.0 40.0 33.0 40.0 8 37.49575 40.0 37.0 40.0 33.0 40.0 9 37.57875 40.0 37.0 40.0 33.0 40.0 10-11 37.519125 37.0 37.0 40.0 33.0 40.0 12-13 37.47175 37.0 37.0 40.0 33.0 40.0 14-15 37.486125 37.0 37.0 40.0 33.0 40.0 16-17 37.470375 37.0 37.0 40.0 33.0 40.0 18-19 37.331500000000005 37.0 37.0 40.0 33.0 40.0 20-21 37.219750000000005 37.0 37.0 40.0 33.0 40.0 22-23 37.19525 37.0 37.0 40.0 33.0 40.0 24-25 37.236625000000004 37.0 37.0 40.0 33.0 40.0 26-27 37.211124999999996 37.0 37.0 40.0 33.0 40.0 28-29 37.078125 37.0 37.0 40.0 33.0 40.0 30-31 36.91525 37.0 37.0 40.0 33.0 40.0 32-33 36.964 37.0 37.0 40.0 33.0 40.0 34-35 36.745374999999996 37.0 37.0 40.0 33.0 40.0 36-37 36.4855 37.0 37.0 40.0 33.0 40.0 38-39 36.40375 37.0 37.0 40.0 33.0 40.0 40-41 36.088875 37.0 37.0 40.0 33.0 40.0 42-43 36.0075 37.0 37.0 40.0 33.0 40.0 44-45 35.90475 37.0 35.0 40.0 33.0 40.0 46-47 35.396375 37.0 33.0 37.0 33.0 40.0 48-49 35.380125 37.0 33.0 37.0 33.0 40.0 50-51 35.292625 37.0 33.0 37.0 33.0 40.0 52-53 35.088875 37.0 33.0 37.0 30.0 40.0 54-55 35.008625 37.0 33.0 37.0 30.0 40.0 56-57 34.692875 37.0 33.0 37.0 27.0 37.0 58-59 34.082499999999996 37.0 33.0 37.0 27.0 37.0 60-61 34.413250000000005 37.0 33.0 37.0 27.0 37.0 62-63 34.31975 37.0 33.0 37.0 27.0 37.0 64-65 34.202 37.0 33.0 37.0 27.0 37.0 66-67 34.293125 37.0 33.0 37.0 27.0 37.0 68-69 33.533500000000004 35.0 33.0 37.0 27.0 37.0 70-71 33.6130294044044 35.0 33.0 37.0 27.0 37.0 72-73 34.02031023802051 37.0 33.0 37.0 27.0 37.0 74-75 33.97158475054454 37.0 33.0 37.0 27.0 37.0 76-77 33.84213334714811 37.0 33.0 37.0 27.0 37.0 78-79 33.83279047931218 37.0 33.0 37.0 27.0 37.0 80-81 33.77152283528105 37.0 33.0 37.0 27.0 37.0 82-83 33.804971913984424 37.0 33.0 37.0 27.0 37.0 84-85 33.74767897449546 37.0 33.0 37.0 27.0 37.0 86-87 33.67573380566802 37.0 33.0 37.0 27.0 37.0 88-89 33.718243927125506 37.0 33.0 37.0 27.0 37.0 90-91 33.52593623481782 37.0 33.0 37.0 27.0 37.0 92-93 33.48608299595142 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 13.0 21 18.0 22 22.0 23 27.0 24 36.0 25 38.0 26 36.0 27 49.0 28 48.0 29 62.0 30 75.0 31 102.0 32 153.0 33 180.0 34 224.0 35 428.0 36 841.0 37 963.0 38 677.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 88.55 2.7 2.075 6.675000000000001 2 74.175 15.85 5.675 4.3 3 34.949999999999996 40.5 12.975 11.575000000000001 4 33.725 27.825 18.099999999999998 20.349999999999998 5 24.85 33.025 23.575 18.55 6 20.05 39.900000000000006 24.275 15.775 7 37.974999999999994 27.85 18.775 15.4 8 32.550000000000004 30.775000000000002 20.599999999999998 16.075 9 27.675 29.049999999999997 24.775 18.5 10-11 26.6 27.400000000000002 26.650000000000002 19.35 12-13 29.5375 25.825 27.037499999999998 17.599999999999998 14-15 22.85 30.25 27.5875 19.3125 16-17 24.7375 30.925000000000004 25.7125 18.625 18-19 24.7375 26.900000000000002 26.237500000000004 22.125 20-21 26.069017254313575 26.456614153538382 26.506626656664167 20.967741935483872 22-23 26.5375 24.337500000000002 26.525 22.6 24-25 26.1625 25.224999999999998 27.8125 20.8 26-27 25.424999999999997 25.637500000000003 29.2375 19.7 28-29 26.775 24.837500000000002 27.500000000000004 20.8875 30-31 25.912499999999998 25.424999999999997 27.1625 21.5 32-33 24.825 26.55 28.15 20.474999999999998 34-35 25.86659992491553 26.21699411838318 27.318233012138656 20.59817294456263 36-37 25.912499999999998 24.275 28.000000000000004 21.8125 38-39 26.525 25.15 28.1 20.225 40-41 26.329620823426353 25.62883243649105 26.454761606807658 21.586785133274937 42-43 25.0375751503006 26.44038076152305 26.91633266533066 21.60571142284569 44-45 23.201101238893756 26.1419096483544 28.231760730822174 22.42522838192967 46-47 25.637500000000003 23.95 28.075 22.3375 48-49 25.89721145429536 25.05939727397774 29.048393147430286 19.99499812429661 50-51 25.9875 25.7125 28.499999999999996 19.8 52-53 24.993742177722154 26.382978723404253 27.584480600750936 21.038798498122656 54-55 25.337500000000002 26.487500000000004 28.537499999999998 19.6375 56-57 25.9875 25.424999999999997 27.750000000000004 20.837500000000002 58-59 24.875 24.675 29.475 20.974999999999998 60-61 24.45 24.9875 29.225 21.337500000000002 62-63 23.5125 25.374999999999996 31.2375 19.875 64-65 23.724999999999998 25.5 30.4375 20.3375 66-67 23.8875 25.05 30.5 20.5625 68-69 23.225 26.8625 28.325 21.587500000000002 70-71 24.83741870935468 25.46273136568284 29.00200100050025 20.69784892446223 72-73 25.739348370927317 24.9749373433584 28.471177944862152 20.81453634085213 74-75 23.99598393574297 26.819779116465863 29.24196787148594 19.942269076305223 76-77 23.916048762096267 25.914289305014453 28.71685308533367 21.452808847555612 78-79 24.386252045826513 24.27294473120987 29.208107767845902 22.132695455117712 80-81 23.883984867591426 27.26355611601513 30.403530895334175 18.448928121059268 82-83 23.39269925476822 24.921055955538716 30.8323860047998 20.853858784893266 84-85 23.58669533324902 23.232578727709623 32.69255090426205 20.48817503477931 86-87 22.406376518218625 25.746457489878544 31.819331983805665 20.027834008097166 88-89 22.115384615384613 26.821862348178136 31.11082995951417 19.951923076923077 90-91 24.886133603238868 25.54402834008097 30.452935222672068 19.116902834008098 92-93 22.57085020242915 28.542510121457486 30.136639676113358 18.75 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 6.5 18 8.0 19 2.0 20 2.5 21 4.5 22 4.0 23 6.0 24 4.5 25 3.0 26 6.5 27 8.5 28 17.5 29 25.5 30 25.0 31 26.5 32 38.5 33 57.5 34 63.0 35 77.0 36 102.0 37 123.5 38 161.0 39 179.0 40 179.5 41 190.0 42 200.5 43 205.0 44 205.5 45 197.0 46 197.0 47 204.0 48 172.0 49 162.0 50 172.5 51 163.5 52 147.5 53 133.0 54 116.5 55 87.0 56 70.0 57 64.5 58 68.0 59 58.0 60 43.0 61 39.5 62 37.5 63 38.5 64 39.5 65 36.5 66 30.0 67 27.5 68 20.5 69 14.0 70 11.5 71 8.0 72 6.5 73 4.5 74 5.0 75 3.5 76 2.0 77 1.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.025 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.11249999999999999 36-37 0.0 38-39 0.0 40-41 0.11249999999999999 42-43 0.2 44-45 0.11249999999999999 46-47 0.0 48-49 0.0375 50-51 0.0 52-53 0.125 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 4.0 71 5.0 72 2.0 73 3.0 74 4.0 75 2.0 76 3.0 77 4.0 78 3.0 79 3.0 80 4.0 81 3.0 82 3.0 83 2.0 84 3.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3952.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.925 #Duplication Level Percentage of deduplicated Percentage of total 1 93.77363980215304 80.575 2 4.131510037823683 7.1 3 0.7855688100087286 2.025 4 0.3200465522257783 1.0999999999999999 5 0.17457084666860634 0.75 6 0.08728542333430317 0.44999999999999996 7 0.08728542333430317 0.525 8 0.17457084666860634 1.2 9 0.08728542333430317 0.675 >10 0.3782368344486471 5.6000000000000005 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 27 0.675 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 26 0.65 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 24 0.6 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 21 0.525 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 19 0.475 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 19 0.475 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 18 0.44999999999999996 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 16 0.4 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 12 0.3 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 12 0.3 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 10 0.25 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 10 0.25 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 10 0.25 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 9 0.22499999999999998 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 9 0.22499999999999998 No Hit GGATTCTTCTTTTTTGTGGGCCATTTGTGGCATGCAGGAAGAGCCCGAGC 9 0.22499999999999998 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 8 0.2 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 8 0.2 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 8 0.2 No Hit GGAATAAGAATAAATCGCAACTCCTTTCCACTACACATAAAAATTGATTT 8 0.2 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 8 0.2 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 8 0.2 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 7 0.17500000000000002 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 7 0.17500000000000002 No Hit GGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTG 7 0.17500000000000002 No Hit GGATTTGAAGAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAG 6 0.15 No Hit GGAGAGGGTGAGCATATATATTTATACGACGAATAAAAGGCTGCCACGTG 6 0.15 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 6 0.15 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 5 0.125 No Hit GGCGCGGCGTAAGCTATGACAATAAAAAGTGTGCTGTACCTGATGTGTCT 5 0.125 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 5 0.125 No Hit GGAGAACGGCGGCTGCGGCTGCAGCACCTGCAAGTGCGGCACCAGCTGCG 5 0.125 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 5 0.125 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307078 READS because READLEN < 1 Read 307078 spots for ERR6133535.sra Written 307078 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra Rejected 307062 READS because READLEN < 1 Read 307062 spots for ERR6133535.sra Written 307062 spots for ERR6133535.sra SRR ids: ['ERR6133535.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_mg59xci2 ERR6133535.sra spots: 6141256 blocks: [[1, 307062], [307063, 614124], [614125, 921186], [921187, 1228248], [1228249, 1535310], [1535311, 1842372], [1842373, 2149434], [2149435, 2456496], [2456497, 2763558], [2763559, 3070620], [3070621, 3377682], [3377683, 3684744], [3684745, 3991806], [3991807, 4298868], [4298869, 4605930], [4605931, 4912992], [4912993, 5220054], [5220055, 5527116], [5527117, 5834178], [5834179, 6141256]] ERR6133535 file size 1362677 ERR6133535 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133535 ERR6133535_1.fastq Input file: ERR6133535_1.fastq trimmed: ERR6133535-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 08:10:38 2024 >> started Sat Dec 7 08:10:57 2024 >> done (19.749s) 6141256 reads processed; of these: 71 ( 0.00%) short reads filtered out after trimming by size control 15 ( 0.00%) empty reads filtered out after trimming by size control 6141170 (100.00%) reads available; of these: 118582 ( 1.93%) trimmed reads available after processing 6022588 (98.07%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 11 0.00% 19 18 0.00% 20 10 0.00% 21 10 0.00% 22 15 0.00% 23 2 0.00% 24 1 0.00% 25 4 0.00% 26 4 0.00% 27 8 0.00% 28 16 0.00% 29 82 0.00% 30 11 0.00% 31 15 0.00% 32 10 0.00% 33 9 0.00% 34 18 0.00% 35 94 0.00% 36 564 0.01% 37 10 0.00% 38 19 0.00% 39 55 0.00% 40 27 0.00% 41 24 0.00% 42 10 0.00% 43 6 0.00% 44 2 0.00% 45 12 0.00% 46 5 0.00% 47 9 0.00% 48 10 0.00% 49 6 0.00% 50 2 0.00% 51 7 0.00% 52 7 0.00% 53 7 0.00% 54 7 0.00% 55 8 0.00% 56 7 0.00% 57 9 0.00% 58 10 0.00% 59 7 0.00% 60 9 0.00% 61 7 0.00% 62 5 0.00% 63 10 0.00% 64 6 0.00% 65 9 0.00% 66 11 0.00% 67 13 0.00% 68 29 0.00% 69 99 0.00% 70 5010 0.08% 71 5298 0.09% 72 5392 0.09% 73 4917 0.08% 74 5168 0.08% 75 5131 0.08% 76 4947 0.08% 77 5128 0.08% 78 5514 0.09% 79 5928 0.10% 80 5747 0.09% 81 5984 0.10% 82 6694 0.11% 83 6581 0.11% 84 6151 0.10% 85 317 0.01% 86 532 0.01% 87 913 0.01% 88 1653 0.03% 89 3086 0.05% 90 6865 0.11% 91 19811 0.32% 92 82333 1.34% 93 5940704 96.74% 6141170 reads passed initial QC criterion=sequence-density sequence-density=0.69 sequence-density-rank=1 fanout-score=4.55 fanout-score-rank=23 prefix-density=0.91 prefix-fanout=3.4 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=124.42 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=6.7 sequence=AAAAGAAGGGGTGTTCCATCTCCGGACGACGATCCTGCCTGCAGAGGAAGACATGCCGGCGATCATGTCGAGCTTCAAGAAGTTCAACGACTCATTCATGGAGCAATACCAAGACTACTCCAGGCTGTGATGTGAAGAGGGAAACAACGAGGTCATCATCGACATGATATATTGCTGCTATTTTCCACCAGCGATTAAAAGTTAAAAAATTTAGCTGTAAGCTGTAACTATCTTGAAGAAACTAAACTGGTTGCTGTGCTTGATATGTAT Started job on | Dec 07 08:12:23 Started mapping on | Dec 07 08:12:24 Finished on | Dec 07 08:13:45 Mapping speed, Million of reads per hour | 272.94 Number of input reads | 6141170 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 4784880 Uniquely mapped reads % | 77.91% Average mapped length | 92.44 Number of splices: Total | 265668 Number of splices: Annotated (sjdb) | 220697 Number of splices: GT/AG | 258198 Number of splices: GC/AG | 4981 Number of splices: AT/AC | 89 Number of splices: Non-canonical | 2400 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.04% Deletion average length | 1.76 Insertion rate per base | 0.02% Insertion average length | 1.93 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1239017 % of reads mapped to multiple loci | 20.18% Number of reads mapped to too many loci | 33460 % of reads mapped to too many loci | 0.54% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.33% % of reads unmapped: other | 0.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 117273 117273 117273 N_multimapping 1239017 1239017 1239017 N_noFeature 289003 333549 4586424 N_ambiguous 172871 18948 490 UnstrandedReadsAssigned:4323006 PositiveStrandReadsAssigned:4432383 NegativeStrandReadsAssigned:197966 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133535 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133535-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 6,141,170 reads, 5,316,459 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,092 rounds 52973 ERR6133535.ke.tsv 35125 ERR6133535.se.tsv 88098 total ==> ERR6133535.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 231 41.848 PNS24243 293 194 0 0 KQK14069 1603 1504 156 25.7806 KQK14071 474 375 0 0 ==> ERR6133535.se.tsv <== BRADI_1g14170v3 155 BRADI_1g53295v3 117 BRADI_1g59795v3 103 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 86 BRADI_1g74790v3 60 BRADI_1g09890v3 0 BRADI_1g77505v3 241 BRADI_1g48960v3 0 ERR6133535 completed mapping pipeline successfully