Starting /dee2/code/volunteer_pipeline.sh ERR6133557 current disk space = 1544488824832 free memory = 1474065540 ERR6133557 SRAfilesize 6a303b57a2d8046b1f40649af062b851 ERR6133557.sra ERR6133557.sra file validated ERR6133557 is single end ERR6133557 is conventional basespace ERR6133557 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133557_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.39575 37.0 33.0 37.0 33.0 37.0 2 36.606 37.0 37.0 37.0 37.0 37.0 3 36.01575 37.0 37.0 37.0 33.0 37.0 4 35.5345 37.0 37.0 37.0 33.0 37.0 5 35.297 37.0 37.0 37.0 33.0 37.0 6 35.74025 37.0 37.0 37.0 33.0 37.0 7 37.57125 40.0 37.0 40.0 33.0 40.0 8 37.61225 40.0 37.0 40.0 33.0 40.0 9 37.77275 40.0 37.0 40.0 33.0 40.0 10-11 37.714124999999996 40.0 37.0 40.0 33.0 40.0 12-13 37.627875 38.5 37.0 40.0 33.0 40.0 14-15 37.595 38.5 37.0 40.0 33.0 40.0 16-17 37.45825 37.0 37.0 40.0 33.0 40.0 18-19 37.458375000000004 38.5 37.0 40.0 33.0 40.0 20-21 37.287125 37.0 37.0 40.0 33.0 40.0 22-23 37.27275 37.0 37.0 40.0 33.0 40.0 24-25 37.424 37.0 37.0 40.0 33.0 40.0 26-27 37.296499999999995 37.0 37.0 40.0 33.0 40.0 28-29 37.23725 37.0 37.0 40.0 33.0 40.0 30-31 37.091875 37.0 37.0 40.0 33.0 40.0 32-33 36.90375 37.0 37.0 40.0 33.0 40.0 34-35 36.813375 37.0 37.0 40.0 33.0 40.0 36-37 36.801625 37.0 37.0 40.0 33.0 40.0 38-39 36.489125 37.0 37.0 40.0 33.0 40.0 40-41 36.31275 37.0 37.0 40.0 33.0 40.0 42-43 36.151375 37.0 37.0 40.0 33.0 40.0 44-45 35.797250000000005 37.0 35.0 38.5 33.0 40.0 46-47 35.615625 37.0 33.0 37.0 33.0 40.0 48-49 35.515249999999995 37.0 33.0 37.0 33.0 40.0 50-51 35.389125 37.0 33.0 37.0 33.0 40.0 52-53 34.96625 37.0 33.0 37.0 30.0 40.0 54-55 34.95325 37.0 33.0 37.0 30.0 38.5 56-57 34.72825 37.0 33.0 37.0 33.0 37.0 58-59 33.536375 35.0 33.0 37.0 27.0 37.0 60-61 34.159000000000006 37.0 33.0 37.0 27.0 37.0 62-63 34.205 37.0 33.0 37.0 27.0 37.0 64-65 34.203125 37.0 33.0 37.0 27.0 37.0 66-67 34.351 37.0 33.0 37.0 27.0 37.0 68-69 33.553 35.0 33.0 37.0 27.0 37.0 70-71 33.640779606910364 35.0 33.0 37.0 27.0 37.0 72-73 34.04759228924506 37.0 33.0 37.0 27.0 37.0 74-75 33.80438165063079 37.0 33.0 37.0 27.0 37.0 76-77 33.98117799999439 37.0 33.0 37.0 27.0 37.0 78-79 34.00593880906516 37.0 33.0 37.0 27.0 37.0 80-81 33.791402084027915 37.0 33.0 37.0 27.0 37.0 82-83 33.808960520483836 37.0 33.0 37.0 27.0 37.0 84-85 33.87186813595317 37.0 33.0 37.0 27.0 37.0 86-87 33.56340718105424 37.0 33.0 37.0 27.0 37.0 88-89 33.80544945250828 37.0 33.0 37.0 27.0 37.0 90-91 33.47682709447416 37.0 33.0 37.0 27.0 37.0 92-93 33.30213903743315 37.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 13.0 21 10.0 22 12.0 23 23.0 24 29.0 25 24.0 26 42.0 27 38.0 28 47.0 29 65.0 30 83.0 31 93.0 32 140.0 33 171.0 34 277.0 35 448.0 36 924.0 37 964.0 38 595.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 88.02499999999999 2.6 2.9749999999999996 6.4 2 71.39999999999999 16.35 6.875000000000001 5.375 3 35.05 39.0 14.075 11.875 4 35.3 27.925 17.724999999999998 19.05 5 26.025 32.4 23.35 18.224999999999998 6 19.125 39.425 25.95 15.5 7 37.075 28.299999999999997 18.625 16.0 8 30.575000000000003 28.375 22.2 18.85 9 25.75 27.85 26.85 19.55 10-11 24.6625 27.35 26.55 21.4375 12-13 28.425 25.1 25.412499999999998 21.0625 14-15 23.849999999999998 30.5375 26.437500000000004 19.175 16-17 26.687499999999996 29.6875 23.775 19.85 18-19 24.8 26.450000000000003 25.0625 23.6875 20-21 25.924999999999997 26.450000000000003 26.275 21.349999999999998 22-23 28.462500000000002 23.3875 26.0125 22.1375 24-25 27.5125 23.799999999999997 26.424999999999997 22.2625 26-27 25.937500000000004 26.275 28.262500000000003 19.525000000000002 28-29 26.787499999999998 26.8125 24.7875 21.6125 30-31 27.962500000000002 25.7 24.8 21.5375 32-33 26.0125 26.474999999999998 25.5375 21.975 34-35 27.275 25.5125 25.2875 21.925 36-37 25.2875 23.849999999999998 26.7625 24.099999999999998 38-39 27.193092228757354 24.32736828932549 29.783506444750348 18.696033037166814 40-41 28.1351689612015 24.993742177722154 25.193992490613265 21.67709637046308 42-43 26.300650325162582 29.177088544272134 23.986993496748372 20.535267633816908 44-45 24.725 25.624999999999996 26.887499999999996 22.7625 46-47 25.5625 22.7125 27.275 24.45 48-49 26.275 23.775 28.299999999999997 21.65 50-51 25.5625 26.1625 28.125 20.150000000000002 52-53 26.419350795839076 26.143627020929944 25.980699335756363 21.45632284747462 54-55 24.2 28.4125 26.7125 20.674999999999997 56-57 27.525 25.650000000000002 26.0625 20.7625 58-59 24.837500000000002 24.474999999999998 28.0625 22.625 60-61 25.974999999999998 23.525 29.262500000000003 21.2375 62-63 22.6375 27.8875 30.012499999999996 19.4625 64-65 24.875 27.5625 26.437500000000004 21.125 66-67 25.44386096524131 28.419604901225306 25.993998499624904 20.142535633908476 68-69 24.0125 26.3 26.825 22.8625 70-71 26.945208906680012 23.53014761070803 26.044533400050035 23.48011008256192 72-73 26.537013801756586 23.73902132998745 27.44040150564617 22.283563362609787 74-75 24.203500818536707 28.38433446669185 27.225790202745248 20.18637451202619 76-77 23.47222222222222 25.063131313131315 28.484848484848484 22.97979797979798 78-79 25.57845492476925 24.64281198634467 28.372739916550767 21.405993172335315 80-81 25.716096324461347 26.932826362484157 28.542458808618505 18.808618504435994 82-83 24.422442244224424 24.143183549124142 28.497080477278498 22.937293729372936 84-85 24.519658989693347 22.738261865377275 30.741824659625905 22.000254485303476 86-87 23.1729055258467 27.48917748917749 28.826075884899417 20.511841100076396 88-89 21.301247771836007 28.635090399796283 29.106187929717343 20.95747389865037 90-91 27.170868347338935 25.515660809778456 27.030812324929972 20.282658517952637 92-93 22.358034122740005 29.055258467023172 27.718360071301245 20.868347338935575 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.0 18 4.5 19 2.0 20 1.0 21 3.0 22 3.0 23 3.0 24 6.5 25 5.0 26 5.0 27 7.0 28 12.0 29 18.5 30 24.5 31 30.0 32 34.0 33 44.5 34 49.5 35 52.0 36 79.0 37 116.0 38 128.5 39 132.0 40 154.0 41 169.5 42 168.5 43 168.5 44 168.5 45 163.5 46 202.5 47 197.0 48 165.5 49 183.5 50 183.5 51 198.5 52 195.5 53 186.5 54 172.5 55 121.0 56 86.5 57 81.0 58 76.5 59 67.5 60 55.5 61 51.5 62 54.5 63 46.0 64 41.0 65 33.5 66 24.5 67 24.5 68 18.0 69 14.0 70 15.0 71 15.5 72 11.5 73 7.0 74 7.0 75 4.0 76 0.5 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.11249999999999999 40-41 0.125 42-43 0.05 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.2625 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.025 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.012672665061462427 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 6.0 71 5.0 72 8.0 73 7.0 74 7.0 75 5.0 76 4.0 77 2.0 78 3.0 79 7.0 80 1.0 81 4.0 82 4.0 83 5.0 84 5.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3927.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.10000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 92.57362355953906 72.3 2 4.3854033290653005 6.8500000000000005 3 1.1523687580025608 2.7 4 0.6402048655569782 2.0 5 0.22407170294494239 0.8750000000000001 6 0.06402048655569782 0.3 7 0.09603072983354673 0.525 8 0.12804097311139565 0.8 9 0.12804097311139565 0.8999999999999999 >10 0.5441741357234315 9.65 >50 0.06402048655569782 3.1 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 64 1.6 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 60 1.5 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 48 1.2 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 35 0.8750000000000001 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 34 0.8500000000000001 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 33 0.8250000000000001 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 28 0.7000000000000001 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 26 0.65 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 25 0.625 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 23 0.575 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 21 0.525 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 20 0.5 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 18 0.44999999999999996 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 16 0.4 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 15 0.375 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 14 0.35000000000000003 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 10 0.25 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 10 0.25 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 10 0.25 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 9 0.22499999999999998 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 9 0.22499999999999998 No Hit GGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGT 9 0.22499999999999998 No Hit GGGCCATTTGTGGCATGCAGGAAGAGCCCGAGCTGCTGCAGCAGGTTTTG 9 0.22499999999999998 No Hit GAAGGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGC 8 0.2 No Hit GGACTGGGTATCCATGCCAGGTGTTATACCAGTAGCTTCAGGTGGTATTC 8 0.2 No Hit GGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAACCCTCTTAACT 8 0.2 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 8 0.2 No Hit GGAAGAGCCCGAGCTGCTGCAGCAGGTTTTGAAAAGGGAATCGATCGTGA 7 0.17500000000000002 No Hit GGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAA 7 0.17500000000000002 No Hit GGTTTTGAAAAGGGAATCGATCGTGATTTAGAACCTGTTCTTTACATGAA 7 0.17500000000000002 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 6 0.15 No Hit GGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTT 6 0.15 No Hit CAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAATACTTGGGCTGATAT 5 0.125 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 5 0.125 No Hit GGAGAACGGCGGCTGCGGCTGCAGCACCTGCAAGTGCGGCACCAGCTGCG 5 0.125 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 5 0.125 No Hit GGAGAAGTACAAGTGCGGATCCAACGTCTTCTGGAAATGGTGAAGATGAT 5 0.125 No Hit GGGTATCCATGCCAGGTGTTATACCAGTAGCTTCAGGTGGTATTCATGTT 5 0.125 No Hit GGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172586 READS because READLEN < 1 Read 172586 spots for ERR6133557.sra Written 172586 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra Rejected 172584 READS because READLEN < 1 Read 172584 spots for ERR6133557.sra Written 172584 spots for ERR6133557.sra SRR ids: ['ERR6133557.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uhmp4v19 ERR6133557.sra spots: 3451682 blocks: [[1, 172584], [172585, 345168], [345169, 517752], [517753, 690336], [690337, 862920], [862921, 1035504], [1035505, 1208088], [1208089, 1380672], [1380673, 1553256], [1553257, 1725840], [1725841, 1898424], [1898425, 2071008], [2071009, 2243592], [2243593, 2416176], [2416177, 2588760], [2588761, 2761344], [2761345, 2933928], [2933929, 3106512], [3106513, 3279096], [3279097, 3451682]] ERR6133557 file size 764135 ERR6133557 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133557 ERR6133557_1.fastq Input file: ERR6133557_1.fastq trimmed: ERR6133557-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 08:34:49 2024 >> started Sat Dec 7 08:34:51 2024 >> done (1.890s) 3451682 reads processed; of these: 152 ( 0.00%) short reads filtered out after trimming by size control 28 ( 0.00%) empty reads filtered out after trimming by size control 3451502 (99.99%) reads available; of these: 72590 ( 2.10%) trimmed reads available after processing 3378912 (97.90%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 50 0.00% 20 21 0.00% 21 20 0.00% 22 24 0.00% 23 12 0.00% 24 6 0.00% 25 9 0.00% 26 7 0.00% 27 6 0.00% 28 20 0.00% 29 30 0.00% 30 12 0.00% 31 8 0.00% 32 27 0.00% 33 17 0.00% 34 9 0.00% 35 110 0.00% 36 458 0.01% 37 20 0.00% 38 30 0.00% 39 71 0.00% 40 69 0.00% 41 29 0.00% 42 7 0.00% 43 14 0.00% 44 15 0.00% 45 9 0.00% 46 8 0.00% 47 7 0.00% 48 8 0.00% 49 8 0.00% 50 7 0.00% 51 29 0.00% 52 18 0.00% 53 8 0.00% 54 5 0.00% 55 4 0.00% 56 7 0.00% 57 13 0.00% 58 4 0.00% 59 7 0.00% 60 18 0.00% 61 10 0.00% 62 1 0.00% 63 7 0.00% 64 3 0.00% 65 2 0.00% 66 4 0.00% 67 5 0.00% 68 20 0.00% 69 58 0.00% 70 5007 0.15% 71 4605 0.13% 72 4945 0.14% 73 4331 0.13% 74 4763 0.14% 75 4782 0.14% 76 4105 0.12% 77 4129 0.12% 78 4581 0.13% 79 5375 0.16% 80 4612 0.13% 81 4977 0.14% 82 5442 0.16% 83 5861 0.17% 84 4906 0.14% 85 146 0.00% 86 292 0.01% 87 486 0.01% 88 869 0.03% 89 1552 0.04% 90 3453 0.10% 91 9856 0.29% 92 53450 1.55% 93 3307587 95.83% 3451502 reads passed initial QC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=5.77 fanout-score-rank=19 prefix-density=1.14 prefix-fanout=2.5 sequence=GCCGCCGCCGCCAAGGAAGGCATGTTCGTCAAGAACTACAGCTACTGATCCTAATCGCATCAAGCTTCAACGCCTGTGAGTGAAAACCAGTGATGAGAGTGCTGCTGCTAGCTAGCGCCGGCATTGATGAGCTTGAGAGGGCACTGTAGCCAGTGTGTCAGTCGTTGTTAAATTACAGGTTGAGATCATCAGCGTACTCCGATGGGAGGTGGACATCAGAAAGTATACTGTGTTTTACCACCCTAATTAAGTAAACAACTTTTGGAATGGTGATCATCTTACTGTTTTAAATAAATCTGTTTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=19 fanout-score=39.50 fanout-score-rank=1 prefix-density=0.63 prefix-fanout=1.7 sequence=CAAGGAAGGCGTCGCCAACGGAACCCTCAAGCTCGTGGGCGGCCACTACGACTTCGTCTCCGGCAAGTTCGACACATGGGAGCTCTAAGTCCTCTCATCCGGTTAACTCCTATACATACAACGTATACTTATACATACAGATATGGAGATGACCCTACAGATCGATCCATTGATGTGGATGCGATGCCATGGAGTATATGTACTCGCTATTTTCCAGTACTGCATGCCGGATGGCTCGATGTGAATTTGTAATAAGCAATAGAAGTTTCTACCATTTTCTGACGTGGGGTTGTACTTGTGATGCGTAATTTGGTCATCTTGTGACCAAAAGACATCAACTATATAATTATAATACCATTTTCATCAAGACTC Started job on | Dec 07 08:35:15 Started mapping on | Dec 07 08:35:15 Finished on | Dec 07 08:35:20 Mapping speed, Million of reads per hour | 2485.08 Number of input reads | 3451502 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 2323144 Uniquely mapped reads % | 67.31% Average mapped length | 92.23 Number of splices: Total | 180727 Number of splices: Annotated (sjdb) | 154625 Number of splices: GT/AG | 175352 Number of splices: GC/AG | 3056 Number of splices: AT/AC | 114 Number of splices: Non-canonical | 2205 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.04% Deletion average length | 1.78 Insertion rate per base | 0.02% Insertion average length | 2.15 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1056396 % of reads mapped to multiple loci | 30.61% Number of reads mapped to too many loci | 21207 % of reads mapped to too many loci | 0.61% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.43% % of reads unmapped: other | 0.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 71962 71962 71962 N_multimapping 1056396 1056396 1056396 N_noFeature 138804 160957 2223007 N_ambiguous 87703 9670 320 UnstrandedReadsAssigned:2096637 PositiveStrandReadsAssigned:2152517 NegativeStrandReadsAssigned:99817 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133557 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133557-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 3,451,502 reads, 2,901,042 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 979 rounds 52973 ERR6133557.ke.tsv 35125 ERR6133557.se.tsv 88098 total ==> ERR6133557.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 79 25.9289 PNS24243 293 194 0 0 KQK14069 1603 1504 36 10.7787 KQK14071 474 375 0 0 ==> ERR6133557.se.tsv <== BRADI_1g14170v3 36 BRADI_1g53295v3 7 BRADI_1g59795v3 16 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 19 BRADI_1g74790v3 17 BRADI_1g09890v3 0 BRADI_1g77505v3 59 BRADI_1g48960v3 0 ERR6133557 completed mapping pipeline successfully