Starting /dee2/code/volunteer_pipeline.sh ERR6133561 current disk space = 1544490643456 free memory = 1604493024 ERR6133561 SRAfilesize 19af08dde2b61f218c149394a50831ea ERR6133561.sra ERR6133561.sra file validated ERR6133561 is single end ERR6133561 is conventional basespace ERR6133561 read1 length is 70-93 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR6133561_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 70-93 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.42725 37.0 33.0 37.0 33.0 37.0 2 36.45575 37.0 37.0 37.0 37.0 37.0 3 36.01475 37.0 37.0 37.0 33.0 37.0 4 35.43825 37.0 37.0 37.0 33.0 37.0 5 35.42725 37.0 37.0 37.0 33.0 37.0 6 35.78875 37.0 37.0 37.0 33.0 37.0 7 37.63475 40.0 37.0 40.0 33.0 40.0 8 37.6915 40.0 37.0 40.0 33.0 40.0 9 37.66425 40.0 37.0 40.0 33.0 40.0 10-11 37.64725 40.0 37.0 40.0 33.0 40.0 12-13 37.51575 38.5 37.0 40.0 33.0 40.0 14-15 37.532125 37.0 37.0 40.0 33.0 40.0 16-17 37.406000000000006 37.0 37.0 40.0 33.0 40.0 18-19 37.353750000000005 37.0 37.0 40.0 33.0 40.0 20-21 37.1585 37.0 37.0 40.0 33.0 40.0 22-23 37.126 37.0 37.0 40.0 33.0 40.0 24-25 37.320625 37.0 37.0 40.0 33.0 40.0 26-27 37.265125 37.0 37.0 40.0 33.0 40.0 28-29 37.223875 37.0 37.0 40.0 33.0 40.0 30-31 37.045875 37.0 37.0 40.0 33.0 40.0 32-33 36.951375 37.0 37.0 40.0 33.0 40.0 34-35 36.781 37.0 37.0 40.0 33.0 40.0 36-37 36.691625 37.0 37.0 40.0 33.0 40.0 38-39 36.43875 37.0 37.0 40.0 33.0 40.0 40-41 36.328 37.0 37.0 40.0 33.0 40.0 42-43 36.129625000000004 37.0 37.0 40.0 33.0 40.0 44-45 35.844125000000005 37.0 35.0 40.0 33.0 40.0 46-47 35.566125 37.0 33.0 37.0 33.0 40.0 48-49 35.560500000000005 37.0 33.0 37.0 33.0 40.0 50-51 35.363749999999996 37.0 33.0 37.0 33.0 40.0 52-53 35.12775 37.0 33.0 37.0 33.0 40.0 54-55 34.909875 37.0 33.0 37.0 27.0 40.0 56-57 34.71725 37.0 33.0 37.0 27.0 38.5 58-59 33.604625 37.0 33.0 37.0 27.0 37.0 60-61 34.178125 37.0 33.0 37.0 27.0 37.0 62-63 34.173500000000004 37.0 33.0 37.0 27.0 37.0 64-65 34.118375 37.0 33.0 37.0 27.0 37.0 66-67 34.32 37.0 33.0 37.0 27.0 37.0 68-69 33.55325 35.0 33.0 37.0 27.0 37.0 70-71 33.61283885692846 35.0 33.0 37.0 27.0 37.0 72-73 34.0799056811997 37.0 33.0 37.0 27.0 37.0 74-75 33.79368306769318 37.0 33.0 37.0 27.0 37.0 76-77 33.92861194896541 37.0 33.0 37.0 27.0 37.0 78-79 33.83878907491662 37.0 33.0 37.0 27.0 37.0 80-81 33.81079010213932 37.0 33.0 37.0 27.0 37.0 82-83 33.61995522724434 37.0 33.0 37.0 27.0 37.0 84-85 33.600783434560554 37.0 33.0 37.0 27.0 37.0 86-87 33.45235085945399 37.0 33.0 37.0 27.0 37.0 88-89 33.543857431749245 37.0 33.0 37.0 27.0 37.0 90-91 33.25631951466127 37.0 33.0 37.0 27.0 37.0 92-93 33.14168351870576 35.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 8.0 21 22.0 22 18.0 23 21.0 24 30.0 25 36.0 26 48.0 27 33.0 28 57.0 29 63.0 30 85.0 31 103.0 32 141.0 33 193.0 34 236.0 35 410.0 36 866.0 37 978.0 38 640.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 85.39999999999999 4.075 3.975 6.550000000000001 2 66.825 19.15 9.025 5.0 3 34.775 34.8 18.15 12.275 4 33.15 27.6 18.4 20.849999999999998 5 24.275 30.099999999999998 27.450000000000003 18.175 6 20.05 40.625 24.3 15.024999999999999 7 35.925000000000004 28.625 19.7 15.75 8 30.599999999999998 27.0 24.275 18.125 9 24.425 27.6 26.950000000000003 21.025 10-11 24.175 28.225 27.200000000000003 20.4 12-13 26.8625 24.2625 27.1375 21.7375 14-15 22.15 29.562500000000004 28.599999999999998 19.6875 16-17 26.400000000000002 28.9375 25.324999999999996 19.3375 18-19 23.3125 26.6125 26.337500000000002 23.7375 20-21 26.987499999999997 26.724999999999998 26.525 19.7625 22-23 28.1625 22.537499999999998 27.425 21.875 24-25 26.375 24.099999999999998 27.375 22.15 26-27 26.424999999999997 25.9625 28.625 18.987499999999997 28-29 26.5875 26.1625 27.025 20.225 30-31 28.9375 25.2125 25.1875 20.6625 32-33 25.15 28.1875 26.075 20.5875 34-35 25.4875 26.5625 26.2125 21.7375 36-37 25.35 24.4875 27.500000000000004 22.662499999999998 38-39 26.5 23.7375 30.4875 19.275000000000002 40-41 26.05 26.325 26.875 20.75 42-43 25.2125 29.037499999999998 25.724999999999998 20.025000000000002 44-45 24.462500000000002 25.3125 29.062500000000004 21.1625 46-47 26.187500000000004 22.787499999999998 27.3375 23.6875 48-49 26.174999999999997 24.2875 28.825 20.7125 50-51 24.45 26.075 27.35 22.125 52-53 25.497808390732622 26.537257357545396 25.685660613650597 22.279273638071384 54-55 24.337500000000002 27.025 28.775000000000002 19.8625 56-57 26.8125 25.924999999999997 27.0125 20.25 58-59 24.1375 24.625 29.812499999999996 21.425 60-61 26.4625 24.962500000000002 27.200000000000003 21.375 62-63 23.0 28.0875 29.8875 19.025 64-65 23.3125 27.3125 28.7 20.674999999999997 66-67 25.640705088136016 27.55344418052256 27.403425428178522 19.402425303162897 68-69 23.724999999999998 26.474999999999998 27.825 21.975 70-71 25.656414103525883 25.756439109777446 26.431607901975497 22.155538884721178 72-73 27.106548140728687 23.200200325528986 27.707524727682486 21.985726806059848 74-75 23.720521826392375 28.311590566984446 27.99799297541395 19.969894631209232 76-77 22.599296128707895 25.527903469079938 29.072398190045252 22.80040221216692 78-79 25.930583501006037 24.886820925553323 29.413983903420522 19.76861167002012 80-81 24.159637416593227 28.679340299634898 28.540853581770115 18.620168702001763 82-83 25.129239692346488 24.56184592106922 28.344471062917663 21.964443323666625 84-85 24.832554025022116 22.709465436623276 29.735877669657523 22.722102868697082 86-87 22.345803842264914 26.02376137512639 31.294236602628917 20.336198179979778 88-89 21.688574317492417 29.00657229524772 29.322548028311424 19.982305358948434 90-91 26.93377148634985 26.276541961577347 28.437815975733066 18.35187057633974 92-93 23.495955510616785 28.993933265925175 28.450455005055613 19.059656218402427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 17.5 18 20.0 19 4.0 20 1.5 21 5.5 22 7.0 23 4.5 24 6.0 25 7.0 26 7.0 27 13.5 28 18.5 29 17.5 30 22.0 31 24.0 32 30.5 33 38.5 34 51.0 35 68.0 36 82.0 37 117.5 38 153.0 39 157.5 40 159.0 41 170.0 42 178.0 43 182.0 44 169.5 45 168.5 46 212.0 47 209.5 48 160.0 49 179.5 50 208.0 51 182.5 52 155.0 53 172.5 54 184.5 55 126.0 56 74.0 57 68.0 58 61.0 59 51.0 60 44.0 61 41.0 62 39.5 63 34.0 64 27.5 65 25.5 66 26.5 67 25.0 68 23.0 69 14.5 70 7.5 71 8.0 72 8.5 73 6.5 74 3.5 75 2.0 76 1.5 77 1.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.1875 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0125 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.012542330364981815 76-77 0.0 78-79 0.0 80-81 0.012588116817724069 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 70 2.0 71 3.0 72 3.0 73 3.0 74 5.0 75 5.0 76 2.0 77 0.0 78 2.0 79 2.0 80 2.0 81 4.0 82 3.0 83 7.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 3956.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.55 #Duplication Level Percentage of deduplicated Percentage of total 1 94.50031426775612 75.175 2 2.95411690760528 4.7 3 0.8170961659333752 1.95 4 0.5342551854179761 1.7000000000000002 5 0.34569453174104336 1.375 6 0.03142677561282212 0.15 7 0.03142677561282212 0.17500000000000002 8 0.09428032683846638 0.6 9 0.03142677561282212 0.22499999999999998 >10 0.5656819610307983 8.799999999999999 >50 0.09428032683846638 5.1499999999999995 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 82 2.0500000000000003 No Hit GGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGT 71 1.775 No Hit GGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGG 53 1.325 No Hit TACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTT 40 1.0 No Hit GGAGTATCCTTGATATATAAATATATAGATAAATAGATTTAAATGGAAAA 33 0.8250000000000001 No Hit GGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTG 33 0.8250000000000001 No Hit GGCGTTCAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTC 25 0.625 No Hit GGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGG 25 0.625 No Hit GGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAA 22 0.5499999999999999 No Hit GGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAA 21 0.525 No Hit GGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTT 19 0.475 No Hit GGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTCGCGTTATTAA 19 0.475 No Hit GGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGC 18 0.44999999999999996 No Hit GGGAGTATTATGAAAGGAGATTAATCATAGATTATCAAAAACCCTAGAAA 15 0.375 No Hit GGGGATGATTCTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTG 15 0.375 No Hit GTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAA 14 0.35000000000000003 No Hit GGTCGCGTTATTAATACTTGGGCTGATATCATCAACCGTGCTAATCTTGG 13 0.325 No Hit GAGGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAAT 10 0.25 No Hit GGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGC 10 0.25 No Hit GTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGT 10 0.25 No Hit GGGGAAGTACACCAGCGACGGCGAGGCCGCCGCCGCCAAGGAAGGCATGT 10 0.25 No Hit GGCCTGTAGTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCG 9 0.22499999999999998 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCG 8 0.2 No Hit GGTAATGAAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGC 8 0.2 No Hit GGGCTGATATCATCAACCGTGCTAATCTTGGTATGGAAGTAATGCACGAA 8 0.2 No Hit GGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAA 7 0.17500000000000002 No Hit GAAAAAAAAGACTTCGATTCATTTTCTATTTATTTCGTTAGTTTTTCTTA 6 0.15 No Hit GGATCGGTTGAAGCGTGCGGCGTCTCTGTCTATGTATTACTGTTTTATGC 5 0.125 No Hit GGGCCATTCACAGACACACACAACTACACAAGAGCTCTCAGCTGCTGCCC 5 0.125 No Hit GAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTG 5 0.125 No Hit GTAGGAATCTGGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCT 5 0.125 No Hit CAACCTAAATGGATTCAATTTCAACCAATCTGTAGTTGATAGTCAAGGTC 5 0.125 No Hit GGTTCACTGCTTTAGGTATTAGTACTATGGCGTTCAACCTAAATGGATTC 5 0.125 No Hit GGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGAGCAGCTTGCAA 5 0.125 No Hit GATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGC 5 0.125 No Hit GGCATATGCCAGCTCTGACCGAAATCTTTGGGGATGATTCTGTATTACAA 5 0.125 No Hit GGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTG 5 0.125 No Hit GGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGCGAAATGGGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra Rejected 80743 READS because READLEN < 1 Read 80743 spots for ERR6133561.sra Written 80743 spots for ERR6133561.sra Rejected 80737 READS because READLEN < 1 Read 80737 spots for ERR6133561.sra Written 80737 spots for ERR6133561.sra SRR ids: ['ERR6133561.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gpjdyucx ERR6133561.sra spots: 1614746 blocks: [[1, 80737], [80738, 161474], [161475, 242211], [242212, 322948], [322949, 403685], [403686, 484422], [484423, 565159], [565160, 645896], [645897, 726633], [726634, 807370], [807371, 888107], [888108, 968844], [968845, 1049581], [1049582, 1130318], [1130319, 1211055], [1211056, 1291792], [1291793, 1372529], [1372530, 1453266], [1453267, 1534003], [1534004, 1614746]] ERR6133561 file size 356676 ERR6133561 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR6133561 ERR6133561_1.fastq Input file: ERR6133561_1.fastq trimmed: ERR6133561-trimmed.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- minimum overlap length for adapter detection (-k): inf -- number of concurrent threads (-t): 20 Sat Dec 7 08:41:49 2024 >> started Sat Dec 7 08:41:50 2024 >> done (0.956s) 1614746 reads processed; of these: 217 ( 0.01%) short reads filtered out after trimming by size control 42 ( 0.00%) empty reads filtered out after trimming by size control 1614487 (99.98%) reads available; of these: 36793 ( 2.28%) trimmed reads available after processing 1577694 (97.72%) untrimmed reads available after processing Length distribution of reads after trimming: length count percentage 18 29 0.00% 19 54 0.00% 20 38 0.00% 21 54 0.00% 22 40 0.00% 23 7 0.00% 24 13 0.00% 25 11 0.00% 26 13 0.00% 27 11 0.00% 28 36 0.00% 29 46 0.00% 30 26 0.00% 31 46 0.00% 32 18 0.00% 33 19 0.00% 34 21 0.00% 35 181 0.01% 36 228 0.01% 37 19 0.00% 38 18 0.00% 39 146 0.01% 40 38 0.00% 41 59 0.00% 42 5 0.00% 43 5 0.00% 44 7 0.00% 45 5 0.00% 46 7 0.00% 47 2 0.00% 48 2 0.00% 49 3 0.00% 50 4 0.00% 51 6 0.00% 52 1 0.00% 53 1 0.00% 54 1 0.00% 55 4 0.00% 56 6 0.00% 57 3 0.00% 58 2 0.00% 59 3 0.00% 60 4 0.00% 61 6 0.00% 62 0 0.00% 63 1 0.00% 64 2 0.00% 65 1 0.00% 66 3 0.00% 67 8 0.00% 68 8 0.00% 69 17 0.00% 70 1489 0.09% 71 1376 0.09% 72 1572 0.10% 73 1380 0.09% 74 1429 0.09% 75 1385 0.09% 76 1294 0.08% 77 1312 0.08% 78 1452 0.09% 79 1562 0.10% 80 1472 0.09% 81 1501 0.09% 82 1685 0.10% 83 1831 0.11% 84 1527 0.09% 85 93 0.01% 86 198 0.01% 87 289 0.02% 88 476 0.03% 89 859 0.05% 90 1811 0.11% 91 4854 0.30% 92 26465 1.64% 93 1555887 96.37% 1614487 reads passed initial QC criterion=sequence-density sequence-density=0.61 sequence-density-rank=1 fanout-score=4.70 fanout-score-rank=21 prefix-density=0.83 prefix-fanout=3.4 sequence=ATGCATGCATGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=29 fanout-score=66.16 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=6.0 sequence=AGGAAGAGGAGGATGCAGTCAGGGCTCACAAACAACCTGCCACTGCCGCCATTGGCCTTCTAAAACAGGAGAGGAGGGGTTAGATAGTTTCGATCTGCAAGGGGGTGGGGCAATGGGCATTCCGTTGAGTTTGTATGGTGGGTGCTGTTTTGCAGAAGCTGTATGCTTATGAGCAGCTATGGTACTTATCGAGGACAGTAGCGTACTTGAGGT Started job on | Dec 07 08:42:02 Started mapping on | Dec 07 08:42:02 Finished on | Dec 07 08:42:05 Mapping speed, Million of reads per hour | 1937.38 Number of input reads | 1614487 Average input read length | 92 UNIQUE READS: Uniquely mapped reads number | 1127459 Uniquely mapped reads % | 69.83% Average mapped length | 92.37 Number of splices: Total | 80462 Number of splices: Annotated (sjdb) | 67694 Number of splices: GT/AG | 78109 Number of splices: GC/AG | 1376 Number of splices: AT/AC | 35 Number of splices: Non-canonical | 942 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.04% Deletion average length | 1.82 Insertion rate per base | 0.02% Insertion average length | 1.91 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 452719 % of reads mapped to multiple loci | 28.04% Number of reads mapped to too many loci | 10605 % of reads mapped to too many loci | 0.66% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.42% % of reads unmapped: other | 0.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 34309 34309 34309 N_multimapping 452719 452719 452719 N_noFeature 62544 73890 1079467 N_ambiguous 41406 4765 92 UnstrandedReadsAssigned:1023509 PositiveStrandReadsAssigned:1048804 NegativeStrandReadsAssigned:47900 Dataset is classified positive stranded MeadianReadLen=93 20thPercentileLength=93 echo kmer=89 ERR6133561 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31 [quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20 [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in single-end mode [quant] will process file 1: ERR6133561-trimmed.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 1,614,487 reads, 1,370,071 reads pseudoaligned [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 997 rounds 52973 ERR6133561.ke.tsv 35125 ERR6133561.se.tsv 88098 total ==> ERR6133561.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 837 0 0 PNS24247 1044 945 0 0 PNS24249 1928 1829 0 0 PNS24246 1044 945 0 0 PNS24248 1044 945 0 0 PNS24244 1471 1372 53 36.6922 PNS24243 293 194 0 0 KQK14069 1603 1504 12 7.57854 KQK14071 474 375 0 0 ==> ERR6133561.se.tsv <== BRADI_1g14170v3 12 BRADI_1g53295v3 28 BRADI_1g59795v3 14 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 20 BRADI_1g74790v3 10 BRADI_1g09890v3 0 BRADI_1g77505v3 31 BRADI_1g48960v3 0 ERR6133561 completed mapping pipeline successfully