Starting /dee2/code/volunteer_pipeline.sh ERR9452510 current disk space = 1547634429952 free memory = 1592662524 ERR9452510 SRAfilesize 8db0fe6e8d0af178c657119f8153be04 ERR9452510.sra ERR9452510.sra file validated ERR9452510 is paired end ERR9452510 is conventional basespace ERR9452510 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR9452510_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.7035 37.0 37.0 37.0 37.0 37.0 2 35.56425 37.0 37.0 37.0 37.0 37.0 3 35.94 37.0 37.0 37.0 37.0 37.0 4 36.0085 37.0 37.0 37.0 37.0 37.0 5 36.0785 37.0 37.0 37.0 37.0 37.0 6 36.0755 37.0 37.0 37.0 37.0 37.0 7 35.988 37.0 37.0 37.0 37.0 37.0 8 36.1005 37.0 37.0 37.0 37.0 37.0 9 36.26 37.0 37.0 37.0 37.0 37.0 10-14 36.2111 37.0 37.0 37.0 37.0 37.0 15-19 36.162 37.0 37.0 37.0 37.0 37.0 20-24 36.1351 37.0 37.0 37.0 37.0 37.0 25-29 36.052400000000006 37.0 37.0 37.0 37.0 37.0 30-34 35.9434 37.0 37.0 37.0 37.0 37.0 35-39 35.9285 37.0 37.0 37.0 37.0 37.0 40-44 35.7635 37.0 37.0 37.0 37.0 37.0 45-49 35.7237 37.0 37.0 37.0 37.0 37.0 50-54 35.778800000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.8296 37.0 37.0 37.0 37.0 37.0 60-64 35.9293 37.0 37.0 37.0 37.0 37.0 65-69 35.821000000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.8327 37.0 37.0 37.0 37.0 37.0 75-79 35.7904 37.0 37.0 37.0 37.0 37.0 80-84 35.771 37.0 37.0 37.0 37.0 37.0 85-89 35.8121 37.0 37.0 37.0 37.0 37.0 90-94 35.702999999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.7031 37.0 37.0 37.0 37.0 37.0 100-104 35.657199999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.5634 37.0 37.0 37.0 37.0 37.0 110-114 35.5884 37.0 37.0 37.0 37.0 37.0 115-119 35.5607 37.0 37.0 37.0 37.0 37.0 120-124 35.544 37.0 37.0 37.0 37.0 37.0 125-129 35.576800000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.4303 37.0 37.0 37.0 37.0 37.0 135-139 35.3033 37.0 37.0 37.0 37.0 37.0 140-144 35.3591 37.0 37.0 37.0 34.6 37.0 145-149 35.3774 37.0 37.0 37.0 37.0 37.0 150 35.394 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 5.0 24 3.0 25 10.0 26 13.0 27 32.0 28 29.0 29 35.0 30 48.0 31 100.0 32 114.0 33 137.0 34 178.0 35 399.0 36 2590.0 37 306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.799999999999997 12.25 14.274999999999999 43.675000000000004 2 27.131782945736433 19.929982495623904 29.43235808952238 23.50587646911728 3 28.299999999999997 25.75 18.775 27.175 4 31.175000000000004 28.725 15.35 24.75 5 28.15 30.15 17.925 23.775 6 22.125 30.675 20.575 26.625 7 21.7 13.950000000000001 35.85 28.499999999999996 8 22.25 18.875 23.375 35.5 9 24.925 18.325 25.775 30.975 10-14 26.474999999999998 23.169999999999998 21.875 28.48 15-19 26.83 22.09 22.814999999999998 28.265 20-24 26.99 23.14 21.86 28.01 25-29 26.655 22.965 21.895 28.485 30-34 26.855 22.39 22.25 28.505000000000003 35-39 26.939999999999998 22.7 22.575 27.785 40-44 27.315 22.720000000000002 21.97 27.994999999999997 45-49 27.485 22.259999999999998 22.225 28.03 50-54 27.67 22.38 21.865000000000002 28.084999999999997 55-59 27.93 22.384999999999998 21.805 27.88 60-64 27.529999999999998 22.03 21.8 28.64 65-69 27.725 21.94 22.025 28.310000000000002 70-74 27.965 22.045 21.51 28.48 75-79 27.72 21.625 21.985 28.67 80-84 27.85 21.81 21.765 28.575 85-89 28.02 22.21 21.565 28.205000000000002 90-94 27.834999999999997 21.88 21.895 28.389999999999997 95-99 28.49 21.855 21.745 27.91 100-104 27.655 22.17 21.785 28.389999999999997 105-109 28.055000000000003 21.665 21.44 28.84 110-114 27.744999999999997 22.23 21.77 28.255000000000003 115-119 28.46 21.990000000000002 21.315 28.235 120-124 28.15 21.715 22.23 27.905 125-129 28.735 21.08 22.23 27.955000000000002 130-134 28.139999999999997 21.505 21.775 28.58 135-139 28.29 21.73 21.86 28.12 140-144 28.555000000000003 22.425 21.38 27.639999999999997 145-149 28.29 21.67 22.040000000000003 28.000000000000004 150 27.750000000000004 21.825 22.15 28.275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.0 23 0.5 24 0.5 25 0.0 26 1.0 27 2.5 28 4.0 29 5.0 30 5.0 31 5.0 32 7.5 33 15.5 34 15.0 35 16.5 36 27.5 37 32.5 38 46.5 39 68.0 40 75.5 41 80.0 42 95.5 43 110.5 44 120.0 45 123.5 46 136.0 47 139.5 48 115.5 49 104.5 50 107.5 51 106.5 52 114.0 53 116.5 54 108.5 55 100.0 56 100.5 57 96.5 58 92.0 59 97.0 60 99.0 61 105.0 62 92.0 63 83.0 64 91.5 65 87.0 66 99.5 67 108.0 68 99.0 69 95.5 70 93.0 71 89.5 72 79.5 73 72.5 74 68.5 75 56.5 76 38.5 77 32.0 78 26.0 79 25.5 80 22.0 81 13.5 82 11.0 83 5.5 84 4.0 85 2.5 86 2.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.025 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.80000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 92.99568965517241 86.3 2 6.330818965517242 11.75 3 0.5926724137931034 1.6500000000000001 4 0.08081896551724138 0.3 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.037500000000000006 0.0 0.0 0.0 0.0 100-101 0.0625 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.15 0.0 0.0 0.0 0.0 108-109 0.225 0.0 0.0 0.0 0.0 110-111 0.225 0.0 0.0 0.0 0.0 112-113 0.225 0.0 0.0 0.0 0.0 114-115 0.2875 0.0 0.0 0.0 0.0 116-117 0.3 0.0 0.0 0.0 0.0 118-119 0.3375 0.0 0.0 0.0 0.0 120-121 0.375 0.0 0.0 0.0 0.0 122-123 0.4125 0.0 0.0 0.0 0.0 124-125 0.44999999999999996 0.0 0.0 0.0 0.0 126-127 0.5 0.0 0.0 0.0 0.0 128-129 0.6125 0.0 0.0 0.0 0.0 130-131 0.675 0.0 0.0 0.0 0.0 132-133 0.8 0.0 0.0 0.0 0.0 134-135 0.8625 0.0 0.0 0.0 0.0 136-137 0.925 0.0 0.0 0.0 0.0 138 0.975 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE ERR9452510 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR9452510_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.936 37.0 37.0 37.0 37.0 37.0 2 35.8275 37.0 37.0 37.0 37.0 37.0 3 35.8825 37.0 37.0 37.0 37.0 37.0 4 35.82 37.0 37.0 37.0 37.0 37.0 5 35.827 37.0 37.0 37.0 37.0 37.0 6 35.8815 37.0 37.0 37.0 37.0 37.0 7 35.837 37.0 37.0 37.0 37.0 37.0 8 35.765 37.0 37.0 37.0 37.0 37.0 9 35.8335 37.0 37.0 37.0 37.0 37.0 10-14 35.9499 37.0 37.0 37.0 37.0 37.0 15-19 35.8583 37.0 37.0 37.0 37.0 37.0 20-24 35.827099999999994 37.0 37.0 37.0 37.0 37.0 25-29 35.9479 37.0 37.0 37.0 37.0 37.0 30-34 35.7988 37.0 37.0 37.0 37.0 37.0 35-39 35.8679 37.0 37.0 37.0 37.0 37.0 40-44 35.8146 37.0 37.0 37.0 37.0 37.0 45-49 35.763999999999996 37.0 37.0 37.0 37.0 37.0 50-54 35.727700000000006 37.0 37.0 37.0 37.0 37.0 55-59 35.801 37.0 37.0 37.0 37.0 37.0 60-64 35.5596 37.0 37.0 37.0 37.0 37.0 65-69 35.712 37.0 37.0 37.0 37.0 37.0 70-74 35.6906 37.0 37.0 37.0 37.0 37.0 75-79 35.573499999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.605999999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.5163 37.0 37.0 37.0 37.0 37.0 90-94 35.4086 37.0 37.0 37.0 37.0 37.0 95-99 35.4464 37.0 37.0 37.0 34.6 37.0 100-104 35.4718 37.0 37.0 37.0 37.0 37.0 105-109 35.43169999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.4285 37.0 37.0 37.0 34.6 37.0 115-119 35.405 37.0 37.0 37.0 37.0 37.0 120-124 35.2149 37.0 37.0 37.0 29.8 37.0 125-129 35.1974 37.0 37.0 37.0 32.2 37.0 130-134 35.0946 37.0 37.0 37.0 27.4 37.0 135-139 35.286899999999996 37.0 37.0 37.0 32.2 37.0 140-144 35.1617 37.0 37.0 37.0 27.4 37.0 145-149 34.900400000000005 37.0 37.0 37.0 25.0 37.0 150 35.3185 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 3.0 21 6.0 22 4.0 23 5.0 24 10.0 25 11.0 26 10.0 27 34.0 28 28.0 29 50.0 30 65.0 31 66.0 32 96.0 33 155.0 34 214.0 35 567.0 36 2478.0 37 197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.549999999999997 12.65 15.25 42.55 2 29.049999999999997 20.525 27.125 23.3 3 26.8 26.924999999999997 17.65 28.625 4 30.725 30.175 14.274999999999999 24.825 5 28.425 30.85 17.45 23.275000000000002 6 22.075 31.4 19.875 26.650000000000002 7 20.849999999999998 14.025000000000002 36.9 28.225 8 23.225 16.725 24.6 35.449999999999996 9 24.099999999999998 17.25 26.325 32.324999999999996 10-14 26.384999999999998 23.96 21.265 28.389999999999997 15-19 27.015 22.035 22.48 28.470000000000002 20-24 26.669999999999998 22.18 22.645 28.505000000000003 25-29 26.43 23.43 21.675 28.465 30-34 26.845000000000002 22.71 22.075 28.37 35-39 27.255000000000003 22.42 22.439999999999998 27.884999999999998 40-44 26.955000000000002 22.3 22.665 28.08 45-49 27.215 22.475 22.0 28.310000000000002 50-54 27.015 21.759999999999998 22.55 28.675 55-59 28.025 22.17 22.045 27.76 60-64 27.779999999999998 21.95 21.945 28.325 65-69 27.544999999999998 22.645 21.595 28.215 70-74 27.96 22.32 21.37 28.349999999999998 75-79 28.155 22.335 21.72 27.79 80-84 28.025 21.85 21.54 28.585 85-89 28.51 22.195 21.14 28.155 90-94 27.685 22.15 21.945 28.22 95-99 27.705000000000002 22.475 21.68 28.139999999999997 100-104 28.735 22.005 20.87 28.389999999999997 105-109 28.139999999999997 22.045 21.51 28.305000000000003 110-114 28.689999999999998 21.89 21.255 28.165000000000003 115-119 28.345 21.7 21.535 28.42 120-124 28.194999999999997 21.97 21.759999999999998 28.075 125-129 28.185 22.355 21.365000000000002 28.095 130-134 28.110000000000003 21.3 22.384999999999998 28.205000000000002 135-139 28.084999999999997 21.759999999999998 22.015 28.139999999999997 140-144 27.894999999999996 22.225 21.6 28.28 145-149 28.73 22.025 21.625 27.62 150 28.749999999999996 22.0 20.974999999999998 28.275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 1.5 26 1.5 27 1.0 28 1.5 29 2.0 30 5.5 31 7.0 32 8.5 33 13.0 34 16.5 35 21.0 36 26.5 37 36.5 38 55.5 39 62.5 40 69.5 41 82.5 42 89.0 43 99.5 44 113.5 45 132.5 46 139.0 47 133.0 48 129.5 49 122.5 50 117.0 51 115.0 52 111.0 53 109.0 54 101.5 55 88.0 56 91.0 57 96.0 58 91.0 59 94.5 60 104.0 61 109.5 62 96.0 63 88.5 64 101.5 65 105.0 66 95.5 67 94.5 68 95.5 69 89.0 70 87.0 71 82.5 72 73.0 73 68.5 74 67.0 75 59.0 76 42.0 77 35.0 78 32.5 79 19.5 80 14.0 81 14.0 82 11.5 83 8.5 84 5.5 85 4.0 86 2.5 87 1.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.27499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 93.48700080407397 87.2 2 5.842937550254623 10.9 3 0.6432591798445457 1.7999999999999998 4 0.02680246582685607 0.1 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.037500000000000006 0.0 0.0 0.0 0.0 100-101 0.0625 0.0 0.0 0.0 0.0 102-103 0.075 0.0 0.0 0.0 0.0 104-105 0.1 0.0 0.0 0.0 0.0 106-107 0.15 0.0 0.0 0.0 0.0125 108-109 0.225 0.0 0.0 0.0 0.025 110-111 0.225 0.0 0.0 0.0 0.025 112-113 0.225 0.0 0.0 0.0 0.025 114-115 0.2875 0.0 0.0 0.0 0.025 116-117 0.3 0.0 0.0 0.0 0.025 118-119 0.3375 0.0 0.0 0.0 0.025 120-121 0.375 0.0 0.0 0.0 0.025 122-123 0.4125 0.0 0.0 0.0 0.025 124-125 0.425 0.0 0.0 0.0 0.025 126-127 0.44999999999999996 0.0 0.0 0.0 0.025 128-129 0.5625 0.0 0.0 0.0 0.025 130-131 0.6 0.0 0.0 0.0 0.025 132-133 0.725 0.0 0.0 0.0 0.025 134-135 0.7875000000000001 0.0 0.0 0.0 0.025 136-137 0.85 0.0 0.0 0.0 0.025 138 0.9 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424553 spots for ERR9452510.sra Written 1424553 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra Read 1424549 spots for ERR9452510.sra Written 1424549 spots for ERR9452510.sra SRR ids: ['ERR9452510.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_dnf6ubxx ERR9452510.sra spots: 28490984 blocks: [[1, 1424549], [1424550, 2849098], [2849099, 4273647], [4273648, 5698196], [5698197, 7122745], [7122746, 8547294], [8547295, 9971843], [9971844, 11396392], [11396393, 12820941], [12820942, 14245490], [14245491, 15670039], [15670040, 17094588], [17094589, 18519137], [18519138, 19943686], [19943687, 21368235], [21368236, 22792784], [22792785, 24217333], [24217334, 25641882], [25641883, 27066431], [27066432, 28490984]] ERR9452510 file size 9577312 ERR9452510 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o ERR9452510 ERR9452510_1.fastq ERR9452510_2.fastq Input file: ERR9452510_1.fastq Paired file: ERR9452510_2.fastq trimmed: ERR9452510-trimmed-pair1.fastq, ERR9452510-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Fri Dec 6 23:56:49 2024 >> started Fri Dec 6 23:57:18 2024 >> done (29.168s) 28490984 read pairs processed; of these: 213 ( 0.00%) short read pairs filtered out after trimming by size control 1336 ( 0.00%) empty read pairs filtered out after trimming by size control 28489435 (99.99%) read pairs available; of these: 536568 ( 1.88%) trimmed read pairs available after processing 27952867 (98.12%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 22 0.00% 19 25 0.00% 20 1473 0.01% 21 23 0.00% 22 18 0.00% 23 15 0.00% 24 21 0.00% 25 12 0.00% 26 16 0.00% 27 20 0.00% 28 19 0.00% 29 28 0.00% 30 67 0.00% 31 54 0.00% 32 15 0.00% 33 31 0.00% 34 17 0.00% 35 28 0.00% 36 29 0.00% 37 30 0.00% 38 32 0.00% 39 24 0.00% 40 24 0.00% 41 29 0.00% 42 29 0.00% 43 24 0.00% 44 43 0.00% 45 40 0.00% 46 58 0.00% 47 41 0.00% 48 54 0.00% 49 57 0.00% 50 86 0.00% 51 56 0.00% 52 56 0.00% 53 90 0.00% 54 90 0.00% 55 93 0.00% 56 99 0.00% 57 126 0.00% 58 120 0.00% 59 159 0.00% 60 183 0.00% 61 171 0.00% 62 223 0.00% 63 189 0.00% 64 261 0.00% 65 220 0.00% 66 234 0.00% 67 292 0.00% 68 296 0.00% 69 330 0.00% 70 356 0.00% 71 359 0.00% 72 412 0.00% 73 399 0.00% 74 445 0.00% 75 499 0.00% 76 460 0.00% 77 526 0.00% 78 566 0.00% 79 649 0.00% 80 664 0.00% 81 710 0.00% 82 748 0.00% 83 805 0.00% 84 826 0.00% 85 866 0.00% 86 961 0.00% 87 1052 0.00% 88 1043 0.00% 89 1163 0.00% 90 1232 0.00% 91 1291 0.00% 92 1468 0.01% 93 1550 0.01% 94 1587 0.01% 95 1749 0.01% 96 1737 0.01% 97 1874 0.01% 98 2022 0.01% 99 2022 0.01% 100 2201 0.01% 101 2435 0.01% 102 2475 0.01% 103 2721 0.01% 104 2712 0.01% 105 2891 0.01% 106 3189 0.01% 107 3237 0.01% 108 3390 0.01% 109 3607 0.01% 110 3649 0.01% 111 3790 0.01% 112 4275 0.02% 113 4481 0.02% 114 4837 0.02% 115 5044 0.02% 116 5246 0.02% 117 5586 0.02% 118 5598 0.02% 119 6114 0.02% 120 6264 0.02% 121 6634 0.02% 122 6955 0.02% 123 7424 0.03% 124 8033 0.03% 125 8229 0.03% 126 8538 0.03% 127 8944 0.03% 128 9238 0.03% 129 9980 0.04% 130 10198 0.04% 131 10902 0.04% 132 11669 0.04% 133 12424 0.04% 134 12697 0.04% 135 13408 0.05% 136 14102 0.05% 137 15114 0.05% 138 15372 0.05% 139 16062 0.06% 140 16931 0.06% 141 17426 0.06% 142 18204 0.06% 143 19716 0.07% 144 20349 0.07% 145 21104 0.07% 146 22754 0.08% 147 23547 0.08% 148 25091 0.09% 149 25948 0.09% 150 27952867 98.12% 28489435 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=3.05 fanout-score-rank=25 prefix-density=0.30 prefix-fanout=2.9 sequence=GGTGTTGTCGAAGCCGATGATGCGGAC criterion=fanout-score sequence-density=0.03 sequence-density-rank=36 fanout-score=824.58 fanout-score-rank=1 prefix-density=1.08 prefix-fanout=22.8 sequence=GCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAGT criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=3.02 fanout-score-rank=26 prefix-density=0.31 prefix-fanout=2.9 sequence=GGTGTTGTCGAAGCCGATGATGCGGAC criterion=fanout-score sequence-density=0.13 sequence-density-rank=27 fanout-score=154.53 fanout-score-rank=1 prefix-density=1.06 prefix-fanout=18.6 sequence=CCGCCGCCGCCA ERR9452510 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 06 23:58:02 Started mapping on | Dec 06 23:58:02 Finished on | Dec 07 00:00:29 Mapping speed, Million of reads per hour | 697.70 Number of input reads | 28489435 Average input read length | 299 UNIQUE READS: Uniquely mapped reads number | 26771087 Uniquely mapped reads % | 93.97% Average mapped length | 298.14 Number of splices: Total | 25288227 Number of splices: Annotated (sjdb) | 23832271 Number of splices: GT/AG | 24942308 Number of splices: GC/AG | 308708 Number of splices: AT/AC | 11559 Number of splices: Non-canonical | 25652 Mismatch rate per base, % | 0.20% Deletion rate per base | 0.00% Deletion average length | 1.68 Insertion rate per base | 0.00% Insertion average length | 1.22 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 320277 % of reads mapped to multiple loci | 1.12% Number of reads mapped to too many loci | 95312 % of reads mapped to too many loci | 0.33% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.35% % of reads unmapped: other | 3.22% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1398071 1398071 1398071 N_multimapping 320277 320277 320277 N_noFeature 890317 13539888 13643997 N_ambiguous 630986 79727 78567 UnstrandedReadsAssigned:25249784 PositiveStrandReadsAssigned:13151472 NegativeStrandReadsAssigned:13048523 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 ERR9452510 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: ERR9452510-trimmed-pair1.fastq ERR9452510-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 28,489,435 reads, 26,338,585 reads pseudoaligned [quant] estimated average fragment length: 241.628 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,163 rounds 52973 ERR9452510.ke.tsv 35125 ERR9452510.se.tsv 88098 total ==> ERR9452510.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 695.635 0 0 PNS24247 1044 803.372 36.111 2.10324 PNS24249 1928 1687.37 442.744 12.2775 PNS24246 1044 803.372 36.111 2.10324 PNS24248 1044 803.372 36.111 2.10324 PNS24244 1471 1230.37 17.9225 0.681599 PNS24243 293 65.7938 66 46.9381 KQK14069 1603 1362.37 18615.5 639.361 KQK14071 474 235.577 1387.92 275.676 ==> ERR9452510.se.tsv <== BRADI_1g14170v3 21159 BRADI_1g53295v3 156 BRADI_1g59795v3 668 BRADI_1g07683v3 0 BRADI_1g00485v3 49 BRADI_1g20270v3 1035 BRADI_1g74790v3 374 BRADI_1g09890v3 5 BRADI_1g77505v3 553 BRADI_1g48960v3 5 ERR9452510 completed mapping pipeline successfully