Starting /dee2/code/volunteer_pipeline.sh SRR11389752 current disk space = 1547256807424 free memory = 1596754280 SRR11389752 SRAfilesize 153813adb0b6fb4de20ac11e7beb825d SRR11389752.sra SRR11389752.sra file validated SRR11389752 is paired end SRR11389752 is conventional basespace SRR11389752 read1 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR11389752_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.73125 32.0 32.0 32.0 32.0 32.0 2 30.7725 32.0 32.0 32.0 32.0 32.0 3 30.5975 32.0 32.0 32.0 32.0 32.0 4 30.7855 32.0 32.0 32.0 32.0 32.0 5 30.7655 32.0 32.0 32.0 32.0 32.0 6 33.82075 36.0 36.0 36.0 32.0 36.0 7 33.914 36.0 36.0 36.0 32.0 36.0 8 33.78075 36.0 36.0 36.0 32.0 36.0 9 33.77725 36.0 36.0 36.0 32.0 36.0 10-11 33.795249999999996 36.0 36.0 36.0 32.0 36.0 12-13 33.87475 36.0 36.0 36.0 32.0 36.0 14-15 33.801249999999996 36.0 36.0 36.0 32.0 36.0 16-17 33.78775 36.0 36.0 36.0 32.0 36.0 18-19 33.947625 36.0 36.0 36.0 32.0 36.0 20-21 33.912375 36.0 36.0 36.0 32.0 36.0 22-23 33.98525 36.0 36.0 36.0 32.0 36.0 24-25 33.772 36.0 36.0 36.0 32.0 36.0 26-27 33.776125 36.0 36.0 36.0 32.0 36.0 28-29 33.560625 36.0 36.0 36.0 29.5 36.0 30-31 33.583625 36.0 36.0 36.0 32.0 36.0 32-33 33.47575 36.0 36.0 36.0 29.5 36.0 34-35 33.508625 36.0 36.0 36.0 27.0 36.0 36-37 33.87683358624178 36.0 36.0 36.0 32.0 36.0 38-39 33.80652503793627 36.0 36.0 36.0 32.0 36.0 40-41 33.7692210419828 36.0 36.0 36.0 29.5 36.0 42-43 33.69094587759231 36.0 36.0 36.0 29.5 36.0 44-45 33.54653515427415 36.0 36.0 36.0 26.5 36.0 46-47 33.62873039959535 36.0 36.0 36.0 29.5 36.0 48-49 33.664011127971676 36.0 36.0 36.0 32.0 36.0 50-51 33.59099256296896 36.0 36.0 36.0 27.0 36.0 52-53 33.545029091828994 36.0 36.0 36.0 26.5 36.0 54-55 33.427829117638325 36.0 36.0 36.0 21.0 36.0 56-57 33.387019230769226 36.0 36.0 36.0 21.0 36.0 58-59 33.411488922383526 36.0 36.0 36.0 24.0 36.0 60-61 33.40668523676881 36.0 36.0 36.0 24.0 36.0 62-63 33.27776174509823 36.0 36.0 36.0 21.0 36.0 64-65 33.28447363231252 36.0 36.0 36.0 17.5 36.0 66-67 33.344844912658864 36.0 36.0 36.0 21.0 36.0 68-69 33.38792489422073 36.0 36.0 36.0 24.0 36.0 70-71 33.134159859905196 36.0 36.0 36.0 17.5 36.0 72-73 33.24064878555167 36.0 36.0 36.0 21.0 36.0 74-75 33.23307020786032 36.0 36.0 36.0 21.0 36.0 76 32.86377358490566 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 5.0 23 4.0 24 16.0 25 18.0 26 36.0 27 64.0 28 105.0 29 135.0 30 194.0 31 241.0 32 313.0 33 492.0 34 866.0 35 1465.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.65048052604957 17.020738492665654 15.275670207384925 35.053110773899846 2 22.660596863935258 15.832068791097623 36.59585230146687 24.911482043500254 3 20.713201820940817 21.901871522508852 26.783004552352047 30.60192210419828 4 25.1896813353566 29.236216489630756 20.35912999494183 25.214972180070816 5 24.355083459787558 32.144663631765305 23.469903894790086 20.030349013657055 6 21.707193515704155 32.29483282674772 26.266464032421478 19.731509625126648 7 16.5402124430956 26.403641881638844 38.08801213960547 18.968133535660094 8 18.740515933232167 24.73444613050076 32.49873545776429 24.02630247850278 9 20.839656044511887 21.699544764795146 33.96560445118867 23.4951947395043 10-11 20.928174001011634 32.39757207890743 24.0642387455741 22.610015174506827 12-13 21.863935255437532 24.911482043500254 28.578654527061204 24.64592817400101 14-15 21.09256449165402 26.833586241780477 28.08548305513404 23.98836621143146 16-17 22.572078907435507 26.960040465351543 26.707132018209407 23.76074860900354 18-19 21.231664137582197 25.78401618614062 28.27516439049064 24.709155285786547 20-21 20.8649468892261 26.997976732422863 28.90743550834598 23.22964087000506 22-23 21.901871522508852 26.5048052604957 27.807283763277695 23.786039453717756 24-25 22.255943348507838 27.111785533636823 26.681841173495197 23.950429944360142 26-27 21.282245827010623 27.035912999494183 26.884167931208903 24.79767324228629 28-29 22.685887708649467 26.6944865958523 27.453211937278706 23.166413758219527 30-31 21.421345472938796 26.820940819423367 27.503793626707136 24.2539200809307 32-33 21.59838138593829 28.287809812847748 26.985331310065757 23.128477491148207 34-35 22.06626201315124 26.125442589782498 26.80829539706626 25.0 36-37 21.56044511886697 26.378351036924634 27.541729893778456 24.519473950429944 38-39 21.699544764795146 26.226605968639355 27.035912999494183 25.03793626707132 40-41 22.205361659079415 26.795649974709157 27.21294891249368 23.786039453717756 42-43 21.56044511886697 26.795649974709157 28.072837632776938 23.57106727364694 44-45 21.889226100151745 25.77137076378351 27.807283763277695 24.53211937278705 46-47 22.344461305007588 26.036924633282755 26.833586241780477 24.785027819929187 48-49 21.75012645422357 25.531107738998482 28.325746079919067 24.393019726858878 50-51 22.435816365245984 24.471986847097508 28.291387378272415 24.80080940938409 52-53 22.43865418669365 24.323298760435115 26.38502403238047 26.853023020490767 54-55 22.26438962681847 25.515496521189117 27.94433902593295 24.275774826059457 56-57 21.31831983805668 25.885627530364374 28.365384615384613 24.430668016194332 58-59 21.27848101265823 25.544303797468356 29.08860759493671 24.088607594936708 60-61 22.499366928336286 24.71511775132945 29.159280830590024 23.62623448974424 62-63 22.558581380620645 24.02786573780874 28.144395186827104 25.26915769474351 64-65 22.582689139526043 25.06653149157268 28.37409707261437 23.976682296286906 66-67 21.610653138871275 25.948002536461633 28.065948002536462 24.375396322130626 68-69 22.195431472081218 24.885786802030456 27.093908629441625 25.824873096446698 70-71 22.457788498159196 25.288815538910754 28.03097625999746 24.222419702932587 72-73 22.94455066921606 25.430210325047803 27.342256214149142 24.282982791587 74-75 23.024891774891778 23.863636363636363 27.786796536796537 25.324675324675322 76 24.037735849056606 0.0 40.867924528301884 35.094339622641506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 46.0 1 23.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 2.0 17 4.5 18 20.5 19 41.0 20 48.0 21 53.5 22 50.5 23 39.0 24 27.0 25 20.5 26 32.5 27 57.5 28 60.5 29 56.5 30 61.0 31 60.5 32 76.5 33 88.5 34 84.5 35 93.5 36 116.0 37 141.0 38 157.0 39 157.5 40 169.0 41 178.0 42 171.0 43 170.0 44 170.0 45 164.5 46 168.0 47 157.5 48 163.0 49 166.5 50 146.0 51 133.0 52 115.5 53 100.0 54 92.5 55 105.5 56 104.5 57 88.0 58 85.0 59 93.5 60 96.0 61 90.5 62 85.5 63 80.0 64 66.5 65 60.5 66 61.0 67 55.5 68 49.0 69 43.0 70 36.0 71 29.5 72 31.5 73 32.0 74 28.0 75 25.5 76 21.0 77 12.0 78 8.0 79 7.5 80 5.5 81 3.5 82 3.5 83 3.0 84 1.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.15 2 1.15 3 1.15 4 1.15 5 1.15 6 1.3 7 1.15 8 1.15 9 1.15 10-11 1.15 12-13 1.15 14-15 1.15 16-17 1.15 18-19 1.15 20-21 1.15 22-23 1.15 24-25 1.15 26-27 1.15 28-29 1.15 30-31 1.15 32-33 1.15 34-35 1.15 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 46.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 1.0 51 0.0 52 0.0 53 0.0 54 1.0 55 0.0 56 0.0 57 1.0 58 2.0 59 0.0 60 0.0 61 1.0 62 1.0 63 1.0 64 1.0 65 2.0 66 1.0 67 1.0 68 2.0 69 0.0 70 1.0 71 6.0 72 19.0 73 61.0 74 312.0 75 890.0 76 2650.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.05 #Duplication Level Percentage of deduplicated Percentage of total 1 95.7801221543587 86.25 2 2.637423653525819 4.75 3 0.6107717934480844 1.6500000000000001 4 0.3053858967240422 1.0999999999999999 5 0.22209883398112162 1.0 6 0.13881177123820101 0.75 7 0.055524708495280406 0.35000000000000003 8 0.055524708495280406 0.4 9 0.0 0.0 >10 0.1943364797334814 3.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 46 1.15 No Hit GCCGAGTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATT 31 0.775 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 22 0.5499999999999999 No Hit GTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTA 16 0.4 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 13 0.325 TruSeq Adapter, Index 8 (97% over 36bp) CCGAGTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATTT 12 0.3 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT 10 0.25 TruSeq Adapter, Index 8 (97% over 36bp) CGTAACTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTC 8 0.2 No Hit CTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGAT 8 0.2 No Hit CAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAAGCTACTGGTATAACACCTGGCATGG 7 0.17500000000000002 No Hit GCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCT 7 0.17500000000000002 No Hit GTAACTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCT 6 0.15 No Hit GGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTTCGTT 6 0.15 No Hit ATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATT 6 0.15 No Hit CCGGGTTGTTGGGAGAGATCACATGCATAAATACAACATGAAACAAACGT 6 0.15 No Hit ATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTC 6 0.15 No Hit AGAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTT 5 0.125 No Hit CCGGAGTTTATATTACATGCCCCTCTCCCAACGATAGTTGTAGTACTCTT 5 0.125 No Hit ATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTAC 5 0.125 No Hit TTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCT 5 0.125 No Hit GAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTTC 5 0.125 No Hit GCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTT 5 0.125 No Hit GTTTATATTACATGCCCCTCTCCCAACGATAGTTGTAGTACTCTTATAAT 5 0.125 No Hit AATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.025 0.0 0.0 0.0 0.0 32 0.025 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.05 0.0 0.0 0.0 0.0 40 0.05 0.0 0.0 0.0 0.0 41 0.05 0.0 0.0 0.0 0.0 42 0.05 0.0 0.0 0.0 0.0 43 0.05 0.0 0.0 0.0 0.0 44 0.05 0.0 0.0 0.0 0.0 45 0.05 0.0 0.0 0.0 0.0 46 0.05 0.0 0.0 0.0 0.0 47 0.05 0.0 0.0 0.0 0.0 48 0.05 0.0 0.0 0.0 0.0 49 0.05 0.0 0.0 0.0 0.0 50 0.05 0.0 0.0 0.0 0.0 51 0.05 0.0 0.0 0.0 0.0 52 0.05 0.0 0.0 0.0 0.0 53 0.05 0.0 0.0 0.0 0.0 54 0.05 0.0 0.0 0.0 0.0 55 0.05 0.0 0.0 0.0 0.0 56 0.05 0.0 0.0 0.0 0.0 57 0.05 0.0 0.0 0.0 0.0 58 0.05 0.0 0.0 0.0 0.0 59 0.05 0.0 0.0 0.0 0.0 60 0.05 0.0 0.0 0.0 0.0 61 0.05 0.0 0.0 0.0 0.0 62 0.05 0.0 0.0 0.0 0.0 63 0.05 0.0 0.0 0.0 0.0 64 0.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR11389752 read2 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR11389752_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.23 32.0 32.0 32.0 21.0 32.0 2 30.016 32.0 32.0 32.0 21.0 32.0 3 29.76925 32.0 32.0 32.0 21.0 32.0 4 29.91 32.0 32.0 32.0 21.0 32.0 5 29.9725 32.0 32.0 32.0 21.0 32.0 6 32.815 36.0 36.0 36.0 21.0 36.0 7 32.986 36.0 36.0 36.0 21.0 36.0 8 32.96975 36.0 36.0 36.0 21.0 36.0 9 32.81525 36.0 36.0 36.0 21.0 36.0 10-11 32.687625 36.0 36.0 36.0 17.5 36.0 12-13 32.864875 36.0 36.0 36.0 21.0 36.0 14-15 32.82225 36.0 36.0 36.0 17.5 36.0 16-17 32.65775 36.0 36.0 36.0 14.0 36.0 18-19 32.640874999999994 36.0 36.0 36.0 14.0 36.0 20-21 32.677375 36.0 36.0 36.0 14.0 36.0 22-23 32.580625 36.0 36.0 36.0 14.0 36.0 24-25 32.52275 36.0 36.0 36.0 14.0 36.0 26-27 32.6335 36.0 36.0 36.0 14.0 36.0 28-29 32.642250000000004 36.0 36.0 36.0 14.0 36.0 30-31 32.479625 36.0 36.0 36.0 14.0 36.0 32-33 32.51575 36.0 36.0 36.0 14.0 36.0 34-35 32.50175 36.0 36.0 36.0 14.0 36.0 36-37 32.82350708502024 36.0 36.0 36.0 14.0 36.0 38-39 32.81996457489879 36.0 36.0 36.0 14.0 36.0 40-41 32.94711538461539 36.0 36.0 36.0 14.0 36.0 42-43 32.72431680161944 36.0 36.0 36.0 14.0 36.0 44-45 32.716219635627525 36.0 36.0 36.0 14.0 36.0 46-47 32.81933198380567 36.0 36.0 36.0 14.0 36.0 48-49 32.559084008097166 36.0 36.0 36.0 14.0 36.0 50-51 32.530699929142116 36.0 36.0 36.0 14.0 36.0 52-53 32.641356618577575 36.0 36.0 36.0 14.0 36.0 54-55 32.47791326663014 36.0 36.0 36.0 14.0 36.0 56-57 32.4206329113924 36.0 36.0 36.0 14.0 36.0 58-59 32.44834767173019 36.0 34.0 36.0 14.0 36.0 60-61 32.22194071446668 36.0 34.0 36.0 14.0 36.0 62-63 32.254720893019 36.0 32.0 36.0 14.0 36.0 64-65 32.10993996473068 36.0 34.0 36.0 14.0 36.0 66-67 32.22099889660293 36.0 32.0 36.0 14.0 36.0 68-69 32.06671792936979 36.0 32.0 36.0 14.0 36.0 70-71 32.09607058882838 36.0 32.0 36.0 14.0 36.0 72-73 31.974598110638823 36.0 32.0 36.0 14.0 36.0 74-75 32.05009180866567 36.0 32.0 36.0 14.0 36.0 76 31.38649592549476 36.0 32.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 0.0 11 1.0 12 1.0 13 0.0 14 3.0 15 26.0 16 16.0 17 13.0 18 17.0 19 11.0 20 7.0 21 17.0 22 21.0 23 32.0 24 41.0 25 50.0 26 56.0 27 104.0 28 113.0 29 173.0 30 203.0 31 266.0 32 331.0 33 496.0 34 842.0 35 1111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.16919959473151 24.39209726443769 11.448834853090172 29.989868287740627 2 32.03342618384401 22.56267409470752 27.32337300582426 18.08052671562421 3 24.620445344129553 28.82085020242915 22.596153846153847 23.96255060728745 4 30.531645569620252 29.822784810126585 18.37974683544304 21.265822784810126 5 29.706477732793523 32.89473684210527 19.230769230769234 18.168016194331983 6 23.355263157894736 38.132591093117405 19.078947368421055 19.4331983805668 7 24.671052631578945 19.30668016194332 33.14777327935223 22.874493927125506 8 24.46808510638298 24.240121580547115 23.93617021276596 27.35562310030395 9 26.01214574898785 23.836032388663966 25.354251012145752 24.79757085020243 10-11 28.211618782432602 29.300088596380203 20.465763827363624 22.022528793823568 12-13 27.07567499049309 22.42362783622766 24.502471796171886 25.998225377107364 14-15 25.123527175978715 26.124414037754974 24.553401748384644 24.19865703788167 16-17 27.288911495422173 24.974567650050865 23.55035605289929 24.186164801627672 18-19 26.43110435663627 25.329280648429588 23.746200607902736 24.493414387031407 20-21 26.807916772392794 25.907130170007616 24.76528799797006 22.519665059629535 22-23 27.53844198754607 24.882450120726904 24.48849917397382 23.09060871775321 24-25 25.753355279817676 25.563433780703974 24.60116485186123 24.08204608761712 26-27 26.379201014584652 25.668991756499683 25.402663284717818 22.549143944197844 28-29 26.45153093634862 26.210138483039003 23.745394486088173 23.5929360945242 30-31 25.931558935361217 26.108998732572875 24.575411913814957 23.38403041825095 32-33 25.639069057611596 25.65178684980288 25.37199542159481 23.337148670990715 34-35 27.427554651753937 25.597356380274526 23.754448398576514 23.22064056939502 36-37 26.07537114579368 25.07296028422789 24.857251617815 23.99441695216343 38-39 26.31712580931827 27.04075155516059 24.070077440649992 22.572045194871144 40-41 25.80073427016078 26.648942904165086 23.61058361817952 23.939739207494622 42-43 25.82361885453624 26.799290420679167 23.859604662949824 23.51748606183477 44-45 26.207071347104293 26.384488657964773 23.78659232036497 23.62184767456596 46-47 26.87341772151899 26.87341772151899 22.645569620253163 23.60759493670886 48-49 26.115618661257606 25.824036511156184 24.27738336713996 23.78296146044625 50-51 24.657707910750506 27.459432048681542 24.290060851926977 23.592799188640974 52-53 26.028611216609697 26.256488163058616 23.446005823521965 24.26889479680972 54-55 25.35818435400025 26.689489032585268 25.07924432610625 22.87308228730823 56-57 26.02235204470409 26.212852425704853 25.374650749301498 22.39014478028956 58-59 26.619343389529725 25.69400430979845 24.350361262517428 23.336291038154393 60-61 26.621810333883456 27.281960137108037 23.359146883331217 22.73708264567729 62-63 26.344495180111622 26.052765093860984 24.315068493150687 23.28767123287671 64-65 25.769916009162635 27.157037414100284 23.301094426062612 23.771952150674473 66-67 26.090273363000637 27.565162110616654 23.343928798474252 23.000635727908456 68-69 25.35569105691057 26.803861788617887 24.58079268292683 23.259654471544717 70-71 24.602670057215512 27.209154481881754 25.352828989192627 22.835346471710107 72-73 24.91367182504157 26.921601227778492 24.67067399923264 23.49405294794731 74-75 24.04445649227433 25.95554350772567 26.07752778530767 23.922472214692327 76 24.902874902874903 0.0 36.868686868686865 38.22843822843823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 48.0 1 24.0 2 0.0 3 0.5 4 1.0 5 1.0 6 0.5 7 1.0 8 2.0 9 1.0 10 0.5 11 1.5 12 2.0 13 2.5 14 2.5 15 2.5 16 3.5 17 3.0 18 18.5 19 27.5 20 18.5 21 16.5 22 19.5 23 21.5 24 21.5 25 25.0 26 27.5 27 28.5 28 32.5 29 37.0 30 40.0 31 48.5 32 56.5 33 57.5 34 67.0 35 80.0 36 101.5 37 124.5 38 125.5 39 124.5 40 135.5 41 149.0 42 152.5 43 149.5 44 150.5 45 150.0 46 160.5 47 158.0 48 148.5 49 163.0 50 175.0 51 164.0 52 127.5 53 111.0 54 121.5 55 122.5 56 110.5 57 112.0 58 119.5 59 114.0 60 103.0 61 106.0 62 112.0 63 100.5 64 84.5 65 81.0 66 73.5 67 64.0 68 65.5 69 62.0 70 62.5 71 58.0 72 46.5 73 35.0 74 29.0 75 28.5 76 26.0 77 23.0 78 16.5 79 11.5 80 11.0 81 9.0 82 5.5 83 5.0 84 5.0 85 6.0 86 4.0 87 0.0 88 0.0 89 0.5 90 1.5 91 2.0 92 2.0 93 2.0 94 1.0 95 1.0 96 2.0 97 1.5 98 1.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.3 2 1.275 3 1.2 4 1.25 5 1.2 6 1.2 7 1.2 8 1.3 9 1.2 10-11 1.2375 12-13 1.3875 14-15 1.3375 16-17 1.7000000000000002 18-19 1.3 20-21 1.4749999999999999 22-23 1.6375000000000002 24-25 1.275 26-27 1.4375 28-29 1.6125 30-31 1.375 32-33 1.7125000000000001 34-35 1.6500000000000001 36-37 0.2909919028340081 38-39 0.3415991902834008 40-41 0.06325910931174089 42-43 0.15182186234817813 44-45 0.1644736842105263 46-47 0.05060728744939271 48-49 0.20242914979757085 50-51 0.1898013412628116 52-53 0.037965072133637055 54-55 0.17719276041007467 56-57 0.3291139240506329 58-59 0.08865248226950355 60-61 0.21535343298707882 62-63 0.08870865543023698 64-65 0.3676936731330037 66-67 0.16501650165016502 68-69 0.0 70-71 0.025422651582560064 72-73 0.0383533623114293 74-75 0.0 76 0.11641443538998836 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 48.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 1.0 51 0.0 52 0.0 53 0.0 54 1.0 55 0.0 56 0.0 57 1.0 58 2.0 59 0.0 60 0.0 61 1.0 62 1.0 63 1.0 64 1.0 65 3.0 66 2.0 67 1.0 68 2.0 69 0.0 70 3.0 71 8.0 72 26.0 73 75.0 74 268.0 75 978.0 76 2577.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.45 #Duplication Level Percentage of deduplicated Percentage of total 1 95.40732640787314 87.25 2 2.952433023510115 5.4 3 0.7927829414980863 2.175 4 0.4100601421541826 1.5 5 0.10934937124111535 0.5 6 0.10934937124111535 0.6 7 0.10934937124111535 0.7000000000000001 8 0.054674685620557675 0.4 9 0.0 0.0 >10 0.054674685620557675 1.4749999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 48 1.2 No Hit TGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGAT 11 0.27499999999999997 No Hit ATCGATTAATAGATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAA 8 0.2 No Hit TTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGT 8 0.2 No Hit TGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTAATAGATAAAACTAAATATG 7 0.17500000000000002 No Hit CTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTATCCGA 7 0.17500000000000002 No Hit CAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAAATTAT 7 0.17500000000000002 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACA 7 0.17500000000000002 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCG 6 0.15 No Hit CCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTT 6 0.15 No Hit AAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCG 6 0.15 No Hit CTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACT 6 0.15 No Hit AGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCGATTAATAGATA 5 0.125 No Hit TTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTA 5 0.125 No Hit GATTAATAGATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAACAA 5 0.125 No Hit CGATTAATAGATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAACA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.05 0.0 0.0 0.0 0.0 40 0.05 0.0 0.0 0.0 0.0 41 0.05 0.0 0.0 0.0 0.0 42 0.05 0.0 0.0 0.0 0.0 43 0.05 0.0 0.0 0.0 0.0 44 0.05 0.0 0.0 0.0 0.0 45 0.05 0.0 0.0 0.0 0.0 46 0.05 0.0 0.0 0.0 0.0 47 0.05 0.0 0.0 0.0 0.0 48 0.05 0.0 0.0 0.0 0.0 49 0.05 0.0 0.0 0.0 0.0 50 0.05 0.0 0.0 0.0 0.0 51 0.05 0.0 0.0 0.0 0.0 52 0.05 0.0 0.0 0.0 0.0 53 0.05 0.0 0.0 0.0 0.0 54 0.05 0.0 0.0 0.0 0.0 55 0.05 0.0 0.0 0.0 0.0 56 0.05 0.0 0.0 0.0 0.0 57 0.05 0.0 0.0 0.0 0.0 58 0.05 0.0 0.0 0.0 0.0 59 0.05 0.0 0.0 0.0 0.0 60 0.05 0.0 0.0 0.0 0.0 61 0.05 0.0 0.0 0.0 0.0 62 0.05 0.0 0.0 0.0 0.0 63 0.05 0.0 0.0 0.0 0.0 64 0.05 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358884 spots for SRR11389752.sra Written 1358884 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra Read 1358882 spots for SRR11389752.sra Written 1358882 spots for SRR11389752.sra SRR ids: ['SRR11389752.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xo0qd148 SRR11389752.sra spots: 27177642 blocks: [[1, 1358882], [1358883, 2717764], [2717765, 4076646], [4076647, 5435528], [5435529, 6794410], [6794411, 8153292], [8153293, 9512174], [9512175, 10871056], [10871057, 12229938], [12229939, 13588820], [13588821, 14947702], [14947703, 16306584], [16306585, 17665466], [17665467, 19024348], [19024349, 20383230], [20383231, 21742112], [21742113, 23100994], [23100995, 24459876], [24459877, 25818758], [25818759, 27177642]] SRR11389752 file size 5150365 SRR11389752 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11389752 SRR11389752_1.fastq SRR11389752_2.fastq Input file: SRR11389752_1.fastq Paired file: SRR11389752_2.fastq trimmed: SRR11389752-trimmed-pair1.fastq, SRR11389752-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 04:05:17 2024 >> started Sat Dec 7 04:05:41 2024 >> done (24.202s) 27177642 read pairs processed; of these: 1529 ( 0.01%) short read pairs filtered out after trimming by size control 770517 ( 2.84%) empty read pairs filtered out after trimming by size control 26405596 (97.16%) read pairs available; of these: 25914 ( 0.10%) trimmed read pairs available after processing 26379682 (99.90%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 549 0.00% 19 20 0.00% 20 695 0.00% 21 20 0.00% 22 878 0.00% 23 26 0.00% 24 987 0.00% 25 28 0.00% 26 951 0.00% 27 25 0.00% 28 829 0.00% 29 20 0.00% 30 632 0.00% 31 17 0.00% 32 511 0.00% 33 17 0.00% 34 354 0.00% 35 426 0.00% 36 1250 0.00% 37 469 0.00% 38 928 0.00% 39 606 0.00% 40 915 0.00% 41 894 0.00% 42 1041 0.00% 43 1137 0.00% 44 1291 0.00% 45 1409 0.01% 46 1550 0.01% 47 1773 0.01% 48 2064 0.01% 49 2123 0.01% 50 2307 0.01% 51 2710 0.01% 52 2749 0.01% 53 3081 0.01% 54 3261 0.01% 55 3684 0.01% 56 5179 0.02% 57 4970 0.02% 58 4987 0.02% 59 5557 0.02% 60 5953 0.02% 61 6086 0.02% 62 6722 0.03% 63 7149 0.03% 64 8054 0.03% 65 8439 0.03% 66 9243 0.04% 67 10304 0.04% 68 10919 0.04% 69 11516 0.04% 70 13074 0.05% 71 16434 0.06% 72 43495 0.16% 73 267823 1.01% 74 1945014 7.37% 75 12381655 46.89% 76 11590796 43.90% 26405596 reads passed initial QC criterion=sequence-density sequence-density=1.18 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=22 prefix-density=1.14 prefix-fanout=2.0 sequence=TTCAAATGTACA criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=20.36 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=4.2 sequence=TAAAAAAAAAGATTGAGCCGAGTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGATAGTATCTACCGGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTTCGTTACGAGCTTGTACACAGGCTTCTAAAGCCACTCGATTAGCTGCTGCACCAGGTGCATTTCCCCAAGGATGTCCTAAAGTTCCTCCACCAAATTGTAATACAGAATCATCCCCAAAGATTTCGGTCAGAGCTGGCATATGCCAAACATGAATACCACCTGAAGCTACTGGTATAACACCTGGCATGGATACCCAGTCCTGAGTGAAAAAGATACCGCGAGCACGATCTTTTTCAATAA criterion=sequence-density sequence-density=0.96 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=23 prefix-density=0.94 prefix-fanout=2.0 sequence=GTGCAGTGCATCAGCTTCATCGCCTTCAAGCCACCGGGTTGTGAGGAGTCCGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=4.81 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=1.0 sequence=AAAAAAATGAAAGAAACAAACGTATTCAATAAGCAAAAGGAGAGAGAGGGATTCGAACCCTCGATAGTTCCTAGAACTATACCGGTTTTCAAGACCGGAGCTATCAACCACTCAGCCATCTCTCCACAGCTTAATCCCTATTTTACTCCTAGAAATAGAACACAGCCATATAAAAATAAAAGGATCTACTAACCTCTAGAAACATCTCAGATGCAAATCCTTTTTCGATATATTTCTGTATACTGTATACACGGATAGAAGATCCGCTATACCCGCTTTTGAAATAAAGACTAAATCCCCTACCCTAATCCCCATATCCAAATAAAAGCGGTAAGTAATAAGTTTTAATTATTAATTAAAGAGAAGAATCAATGGATTCATGATTAAACCCCTCCTACTTCTTGTATTTTTTACAATTTAGGTTAAGTGAGGGATCAAATATGTAGTCAACTTTATTTGATGATAGCTTGGAGGATTAGAAATATGACTATTGCTTTCCAATTAGCTGTTT SRR11389752 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 04:06:08 Started mapping on | Dec 07 04:06:08 Finished on | Dec 07 04:09:39 Mapping speed, Million of reads per hour | 450.52 Number of input reads | 26405596 Average input read length | 150 UNIQUE READS: Uniquely mapped reads number | 19247077 Uniquely mapped reads % | 72.89% Average mapped length | 150.22 Number of splices: Total | 4791217 Number of splices: Annotated (sjdb) | 4507048 Number of splices: GT/AG | 4727186 Number of splices: GC/AG | 52607 Number of splices: AT/AC | 994 Number of splices: Non-canonical | 10430 Mismatch rate per base, % | 0.55% Deletion rate per base | 0.01% Deletion average length | 1.58 Insertion rate per base | 0.01% Insertion average length | 1.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 4462479 % of reads mapped to multiple loci | 16.90% Number of reads mapped to too many loci | 82215 % of reads mapped to too many loci | 0.31% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.77% % of reads unmapped: other | 1.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2696048 2696048 2696048 N_multimapping 4462479 4462479 4462479 N_noFeature 938394 18595497 1164898 N_ambiguous 634017 4333 236800 UnstrandedReadsAssigned:17674666 PositiveStrandReadsAssigned:647247 NegativeStrandReadsAssigned:17845379 Dataset is classified negative stranded MeadianReadLen=76 20thPercentileLength=75 echo kmer=71 SRR11389752 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR11389752-trimmed-pair1.fastq SRR11389752-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,405,596 reads, 21,677,753 reads pseudoaligned [quant] estimated average fragment length: 183.086 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,148 rounds 52973 SRR11389752.ke.tsv 35125 SRR11389752.se.tsv 88098 total ==> SRR11389752.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 753.937 4.1062e-08 3.35919e-09 PNS24247 1044 861.914 4.7969 0.343262 PNS24249 1928 1745.91 11.168 0.394531 PNS24246 1044 861.914 4.7969 0.343262 PNS24248 1044 861.914 4.7969 0.343262 PNS24244 1471 1288.91 107.441 5.14134 PNS24243 293 120.291 0 0 KQK14069 1603 1420.91 51.6387 2.24149 KQK14071 474 292.931 2.08846 0.439733 ==> SRR11389752.se.tsv <== BRADI_1g14170v3 63 BRADI_1g53295v3 4 BRADI_1g59795v3 726 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 191 BRADI_1g74790v3 136 BRADI_1g09890v3 0 BRADI_1g77505v3 216 BRADI_1g48960v3 0 SRR11389752 completed mapping pipeline successfully