Starting /dee2/code/volunteer_pipeline.sh SRR11389805 current disk space = 1544936271872 free memory = 1451320472 SRR11389805 SRAfilesize ce47b96c305400fb187583a175d6f1cf SRR11389805.sra SRR11389805.sra file validated SRR11389805 is paired end SRR11389805 is conventional basespace SRR11389805 read1 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR11389805_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0165 32.0 32.0 32.0 32.0 32.0 2 31.0585 32.0 32.0 32.0 32.0 32.0 3 31.1175 32.0 32.0 32.0 32.0 32.0 4 31.21925 32.0 32.0 32.0 32.0 32.0 5 31.18275 32.0 32.0 32.0 32.0 32.0 6 34.30975 36.0 36.0 36.0 32.0 36.0 7 34.42625 36.0 36.0 36.0 32.0 36.0 8 34.44975 36.0 36.0 36.0 32.0 36.0 9 34.35525 36.0 36.0 36.0 32.0 36.0 10-11 34.27425 36.0 36.0 36.0 32.0 36.0 12-13 34.409875 36.0 36.0 36.0 32.0 36.0 14-15 34.333 36.0 36.0 36.0 32.0 36.0 16-17 34.241875 36.0 36.0 36.0 32.0 36.0 18-19 34.300250000000005 36.0 36.0 36.0 32.0 36.0 20-21 34.03325 36.0 36.0 36.0 32.0 36.0 22-23 34.13825 36.0 36.0 36.0 32.0 36.0 24-25 34.035375 36.0 36.0 36.0 32.0 36.0 26-27 33.871750000000006 36.0 36.0 36.0 32.0 36.0 28-29 33.791124999999994 36.0 36.0 36.0 32.0 36.0 30-31 33.870125 36.0 36.0 36.0 32.0 36.0 32-33 33.725 36.0 36.0 36.0 32.0 36.0 34-35 33.67975 36.0 36.0 36.0 29.5 36.0 36-37 34.01583312390048 36.0 36.0 36.0 32.0 36.0 38-39 33.75018848957025 36.0 36.0 36.0 29.5 36.0 40-41 33.674918321186226 36.0 36.0 36.0 24.0 36.0 42-43 33.31892435285248 36.0 36.0 36.0 20.5 36.0 44-45 32.88213118874089 36.0 36.0 36.0 17.5 36.0 46-47 33.50037697914048 36.0 36.0 36.0 27.0 36.0 48-49 33.43063583815029 36.0 36.0 36.0 21.0 36.0 50-51 33.25596883639105 36.0 36.0 36.0 21.0 36.0 52-53 33.18173095402446 36.0 36.0 36.0 21.0 36.0 54-55 32.77762983687121 36.0 32.0 36.0 14.0 36.0 56-57 32.65743125915938 36.0 32.0 36.0 14.0 36.0 58-59 32.711089316633505 36.0 34.0 36.0 14.0 36.0 60-61 32.69094729999228 36.0 32.0 36.0 14.0 36.0 62-63 32.20740927419355 36.0 32.0 36.0 14.0 36.0 64-65 32.41712438416003 36.0 32.0 36.0 14.0 36.0 66-67 32.46594598625124 36.0 32.0 36.0 14.0 36.0 68-69 32.43945904954499 36.0 32.0 36.0 14.0 36.0 70-71 32.383084520081184 36.0 32.0 36.0 14.0 36.0 72-73 31.694158366286977 36.0 32.0 36.0 14.0 36.0 74-75 31.144611501086256 36.0 32.0 36.0 14.0 36.0 76 30.433856051467632 36.0 27.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 4.0 22 10.0 23 16.0 24 13.0 25 42.0 26 51.0 27 67.0 28 117.0 29 122.0 30 184.0 31 267.0 32 380.0 33 574.0 34 1009.0 35 1123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.11183714501131 12.917818547373711 11.661221412415179 30.309122895199796 2 19.85423473234481 23.196783111334508 33.80246293038452 23.146519225936167 3 19.82910278964564 18.899220909776325 35.00879617994471 26.262880120633326 4 23.121387283236995 25.20733852726816 19.47725559185725 32.194018597637594 5 31.842171399849207 28.776074390550388 21.915054033676803 17.4667001759236 6 28.65776882397381 28.355577940065473 24.729287333165452 18.257365902795268 7 13.923096255340539 32.84744910781603 35.813018346318174 17.416436290525258 8 16.034179442070872 30.208595124403114 29.85674792661473 23.900477506911283 9 28.047248052274444 19.47725559185725 29.680824327720533 22.794672028147776 10-11 23.309876853480773 34.38049761246545 22.354863030912288 19.95476250314149 12-13 19.590349334003516 28.286001507916563 25.87333500879618 26.25031414928374 14-15 18.29605428499623 29.617994470972608 26.011560693641616 26.07439055038954 16-17 24.579039959788894 24.013571249057552 25.018848957024375 26.388539834129176 18-19 19.42699170645891 24.42824830359387 30.196029153053534 25.94873083689369 20-21 24.252324704699674 24.616737873837646 30.309122895199796 20.82181452626288 22-23 19.555108709312556 33.95752167902476 25.80118134975493 20.686188261907752 24-25 19.590349334003516 23.56119628047248 29.831615983915555 27.016838401608446 26-27 19.14037954002765 23.35050898579867 25.587532989820282 31.921578484353404 28-29 24.764299182903834 28.61093651791326 24.802011313639223 21.822752985543683 30-31 19.51740605755938 24.242805077290434 30.702526077667464 25.537262787482717 32-33 19.444444444444446 30.11563599798894 24.62292609351433 25.816993464052292 34-35 19.934640522875817 23.95676219205631 29.575163398692812 26.533433886375065 36-37 24.39361568430313 29.60914917682544 24.242805077290434 21.754430061580997 38-39 24.981151042975622 29.467202814777583 23.51093239507414 22.040713747172656 40-41 19.489821563206835 23.284744910781605 30.33425483789897 26.891178688112593 42-43 19.92963056044232 27.821060567981903 30.397084694646896 21.85222417692888 44-45 19.288766021613473 22.945463684342798 29.944709726061824 27.821060567981903 46-47 24.893189243528525 23.309876853480773 24.93088715757728 26.86604674541342 48-49 19.36416184971098 28.713244533802463 31.06308117617492 20.859512440311637 50-51 24.50364413169138 23.02085951244031 30.03267152550892 22.44282483035939 52-53 19.341460349377908 23.161995727032803 24.16739977378409 33.3291441498052 54-55 24.764299182903834 23.670647391577624 29.201759899434315 22.363293526084224 56-57 19.927034847150583 23.512391495785636 29.45024531387596 27.110328343187824 58-59 19.899307740717433 23.511642542479546 30.54751415984896 26.04153555695406 60-61 24.319556451612904 23.500504032258064 29.96471774193548 22.215221774193548 62-63 20.161290322580644 22.101814516129032 31.72883064516129 26.008064516129032 64-65 25.100908173562058 22.46468213925328 30.3481331987891 22.08627648839556 66-67 19.434272004040913 33.69112261649198 24.220229826998356 22.654375552468746 68-69 20.20227560050569 33.1858407079646 24.209860935524652 22.402022756005056 70-71 19.956918398378104 32.58996452103396 24.974657881398883 22.478459199189054 72-73 19.448698315467077 32.72077590607453 25.331801939765185 22.49872383869321 74-75 20.638002773925106 28.765603328710128 26.92094313453537 23.675450762829403 76 24.809006835544835 0.0 41.25452352231604 33.93646964213912 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29.0 1 14.5 2 1.5 3 2.0 4 1.0 5 0.5 6 1.0 7 1.0 8 0.0 9 0.0 10 1.5 11 2.0 12 1.0 13 2.5 14 2.5 15 0.5 16 1.5 17 4.0 18 18.5 19 32.5 20 35.5 21 42.0 22 48.0 23 40.5 24 26.5 25 20.5 26 27.5 27 39.0 28 41.0 29 43.0 30 53.5 31 53.5 32 56.5 33 69.5 34 82.0 35 103.5 36 111.5 37 111.5 38 121.0 39 130.0 40 137.0 41 147.0 42 157.5 43 172.5 44 208.5 45 337.5 46 403.5 47 273.0 48 180.0 49 167.0 50 140.0 51 121.5 52 120.0 53 105.5 54 87.0 55 103.5 56 113.0 57 102.0 58 94.5 59 86.5 60 76.5 61 72.0 62 70.0 63 61.0 64 50.5 65 50.0 66 43.0 67 30.0 68 31.0 69 39.0 70 38.0 71 29.0 72 19.5 73 13.5 74 16.0 75 14.5 76 12.0 77 12.5 78 8.5 79 4.5 80 3.5 81 4.0 82 4.0 83 2.0 84 3.0 85 2.5 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.525 2 0.525 3 0.525 4 0.525 5 0.525 6 0.7250000000000001 7 0.525 8 0.525 9 0.525 10-11 0.525 12-13 0.525 14-15 0.525 16-17 0.525 18-19 0.525 20-21 0.525 22-23 0.5375 24-25 0.525 26-27 0.5375 28-29 0.5625 30-31 0.5375 32-33 0.5499999999999999 34-35 0.5499999999999999 36-37 0.012565971349585321 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.012599218848431397 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 21.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 1.0 53 0.0 54 1.0 55 2.0 56 1.0 57 1.0 58 1.0 59 3.0 60 1.0 61 0.0 62 0.0 63 3.0 64 2.0 65 2.0 66 3.0 67 2.0 68 2.0 69 7.0 70 2.0 71 16.0 72 22.0 73 65.0 74 474.0 75 881.0 76 2487.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.025 #Duplication Level Percentage of deduplicated Percentage of total 1 95.12049985123475 79.925 2 3.1835763165724487 5.35 3 0.7140731925022314 1.7999999999999998 4 0.2677774471883368 0.8999999999999999 5 0.2677774471883368 1.125 6 0.08925914906277893 0.44999999999999996 7 0.0 0.0 8 0.08925914906277893 0.6 9 0.0 0.0 >10 0.20827134781315082 3.35 >50 0.02975304968759298 1.8499999999999999 >100 0.02975304968759298 4.65 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 186 4.65 TruSeq Adapter, Index 19 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT 74 1.8499999999999999 TruSeq Adapter, Index 19 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 45 1.125 TruSeq Adapter, Index 19 (97% over 38bp) GCCGAGTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATT 21 0.525 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 21 0.525 No Hit GTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTT 14 0.35000000000000003 No Hit CCGAGTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATTT 12 0.3 No Hit GTTTAATTGCAGTCAATTAGAAGAATAAAGAAGAATTACTGCATTTCGTA 11 0.27499999999999997 No Hit ATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATT 10 0.25 No Hit GTAACTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCT 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT 8 0.2 TruSeq Adapter, Index 19 (97% over 38bp) GAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTTC 8 0.2 No Hit CTTATTTTTTCTCTTTTTATTTTGTTTCTTTTTATTTAGACCTTCTTCATATTTAGTTTTATCTATTAATCGAT 6 0.15 No Hit GGCGCGAACTCGAATTTGATCGCCTTCCATACTTCACAAGCTGCGGCTAG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT 6 0.15 TruSeq Adapter, Index 19 (97% over 38bp) GCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCAAGATCGCGCCCTT 5 0.125 No Hit AGAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTTTATTTTGTTT 5 0.125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGC 5 0.125 No Hit GAAAAATAGAAAACTCAAAGCAATGTCAGAGTTGACAAAACTGAGCTGAC 5 0.125 No Hit GAAAAAGGTACATAAAATCGGTGTGACCCGTTCTTGTATTATAAGAAATC 5 0.125 No Hit CTTCACAAGCTGCGGCTAGTTCAGGACTCCATTTGCAAGCTGCTCGGATAATTTCATTACCTTCACGAGCA 5 0.125 No Hit AGAAGAATAAAGAAGAATTACTGCATTTCGTAACTTATTTTTTCTCTTTT 5 0.125 No Hit CAACAGATCAATCCAGATCAGTGAGCTGCTGTTTAGGCCTTGCCGGACTCCTCGCAGCCTGGAGGCTTGAAGGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.025 0.0 0.0 0.0 0.0 20 0.025 0.0 0.0 0.0 0.0 21 0.025 0.0 0.0 0.0 0.0 22 0.025 0.0 0.0 0.0 0.0 23 0.025 0.0 0.0 0.0 0.0 24 0.025 0.0 0.0 0.0 0.0 25 0.025 0.0 0.0 0.0 0.0 26 0.025 0.0 0.0 0.0 0.0 27 0.025 0.0 0.0 0.0 0.0 28 0.025 0.0 0.0 0.0 0.0 29 0.025 0.0 0.0 0.0 0.0 30 0.025 0.0 0.0 0.0 0.0 31 0.025 0.0 0.0 0.0 0.0 32 0.025 0.0 0.0 0.0 0.0 33 0.025 0.0 0.0 0.0 0.0 34 0.025 0.0 0.0 0.0 0.0 35 0.025 0.0 0.0 0.0 0.0 36 0.025 0.0 0.0 0.0 0.0 37 0.025 0.0 0.0 0.0 0.0 38 0.025 0.0 0.0 0.0 0.0 39 0.025 0.0 0.0 0.0 0.0 40 0.025 0.0 0.0 0.0 0.0 41 0.025 0.0 0.0 0.0 0.0 42 0.025 0.0 0.0 0.0 0.0 43 0.025 0.0 0.0 0.0 0.0 44 0.025 0.0 0.0 0.0 0.0 45 0.025 0.0 0.0 0.0 0.0 46 0.025 0.0 0.0 0.0 0.0 47 0.025 0.0 0.0 0.0 0.0 48 0.025 0.0 0.0 0.0 0.0 49 0.025 0.0 0.0 0.0 0.0 50 0.025 0.0 0.0 0.0 0.0 51 0.025 0.0 0.0 0.0 0.0 52 0.025 0.0 0.0 0.0 0.0 53 0.025 0.0 0.0 0.0 0.0 54 0.025 0.0 0.0 0.0 0.0 55 0.025 0.0 0.0 0.0 0.0 56 0.025 0.0 0.0 0.0 0.0 57 0.025 0.0 0.0 0.0 0.0 58 0.025 0.0 0.0 0.0 0.0 59 0.025 0.0 0.0 0.0 0.0 60 0.025 0.0 0.0 0.0 0.0 61 0.025 0.0 0.0 0.0 0.0 62 0.025 0.0 0.0 0.0 0.0 63 0.025 0.0 0.0 0.0 0.0 64 0.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACGTC 50 0.0 69.3718 13 ATCTCGT 50 0.0 69.3718 42 CAGTCAC 50 0.0 69.3718 27 ACGTCTG 50 0.0 69.3718 15 CCAGTCA 50 0.0 69.3718 26 AGCTATC 50 0.0 69.3718 38 GAAGCTA 50 0.0 69.3718 36 CACGTCT 50 0.0 69.3718 14 CTCCAGT 50 0.0 69.3718 24 CCTGAAG 50 0.0 69.3718 33 GTCTGAA 50 0.0 69.3718 17 ACCTGAA 50 0.0 69.3718 32 TATCTCG 50 0.0 69.3718 41 TGAAGCT 50 0.0 69.3718 35 TCTGAAC 50 0.0 69.3718 18 TCGTATG 50 0.0 69.3718 45 AAGCTAT 50 0.0 69.3718 37 CTCGTAT 50 0.0 69.3718 44 GTCACCT 45 3.6379788E-12 69.371796 29 GCTATCT 45 3.6379788E-12 69.371796 39 >>END_MODULE SRR11389805 read2 length is 35-76 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR11389805_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 35-76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.71075 32.0 32.0 32.0 32.0 32.0 2 30.32425 32.0 32.0 32.0 32.0 32.0 3 30.125 32.0 32.0 32.0 21.0 32.0 4 30.18075 32.0 32.0 32.0 21.0 32.0 5 30.14225 32.0 32.0 32.0 21.0 32.0 6 33.246 36.0 36.0 36.0 21.0 36.0 7 32.9065 36.0 36.0 36.0 14.0 36.0 8 32.44275 36.0 32.0 36.0 14.0 36.0 9 32.138 36.0 32.0 36.0 14.0 36.0 10-11 32.203625 36.0 32.0 36.0 14.0 36.0 12-13 32.230125 36.0 32.0 36.0 14.0 36.0 14-15 32.129625000000004 36.0 34.0 36.0 14.0 36.0 16-17 32.17975 36.0 34.0 36.0 14.0 36.0 18-19 31.9165 36.0 32.0 36.0 14.0 36.0 20-21 31.60925 36.0 32.0 36.0 14.0 36.0 22-23 31.79925 36.0 32.0 36.0 14.0 36.0 24-25 31.583750000000002 36.0 32.0 36.0 14.0 36.0 26-27 31.1775 36.0 32.0 36.0 14.0 36.0 28-29 30.974 36.0 32.0 36.0 14.0 36.0 30-31 31.05375 36.0 32.0 36.0 14.0 36.0 32-33 31.151375 36.0 32.0 36.0 14.0 36.0 34-35 31.129875 36.0 32.0 36.0 14.0 36.0 36-37 31.381413480885314 36.0 32.0 36.0 14.0 36.0 38-39 31.236292756539235 36.0 32.0 36.0 14.0 36.0 40-41 31.295523138832998 36.0 32.0 36.0 14.0 36.0 42-43 31.2000754527163 36.0 32.0 36.0 14.0 36.0 44-45 31.12487424547284 36.0 32.0 36.0 14.0 36.0 46-47 30.96290241448692 36.0 32.0 36.0 14.0 36.0 48-49 31.07004527162978 36.0 32.0 36.0 14.0 36.0 50-51 30.798667002012074 36.0 32.0 36.0 14.0 36.0 52-53 30.675261094871114 36.0 32.0 36.0 14.0 36.0 54-55 30.69958782400066 36.0 32.0 36.0 14.0 36.0 56-57 30.701624383781407 36.0 32.0 36.0 14.0 36.0 58-59 30.510269354499894 36.0 27.0 36.0 14.0 36.0 60-61 30.484028746234543 36.0 29.5 36.0 14.0 36.0 62-63 30.46469104665826 36.0 29.5 36.0 14.0 36.0 64-65 30.287259903425984 36.0 27.0 36.0 14.0 36.0 66-67 30.187049353227856 36.0 27.0 36.0 14.0 36.0 68-69 30.08083647115823 36.0 27.0 36.0 14.0 36.0 70-71 30.11739431137532 36.0 27.0 36.0 14.0 36.0 72-73 30.09475266496831 36.0 27.0 36.0 14.0 36.0 74-75 30.2422353988926 36.0 27.0 36.0 14.0 36.0 76 30.207688908528503 36.0 27.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 0.0 12 3.0 13 2.0 14 5.0 15 26.0 16 144.0 17 147.0 18 88.0 19 33.0 20 21.0 21 17.0 22 19.0 23 13.0 24 34.0 25 45.0 26 60.0 27 72.0 28 99.0 29 113.0 30 161.0 31 223.0 32 347.0 33 566.0 34 879.0 35 858.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.324269889224574 19.461228600201412 9.466263846928499 24.74823766364552 2 35.279315551082036 21.238047307498743 26.547559134373426 16.9350780070458 3 33.07344064386318 24.798792756539235 21.378269617706238 20.74949698189135 4 35.46277665995976 28.57142857142857 17.50503018108652 18.46076458752515 5 35.26156941649899 30.533199195171022 18.209255533199194 15.995975855130784 6 31.31287726358149 32.62072434607646 19.13983903420523 16.92655935613682 7 30.05533199195171 16.37323943661972 33.24949698189135 20.321931589537222 8 30.3472571716155 21.640664318067437 23.100150981378963 24.911927528938097 9 32.15903371917464 21.917463512833418 23.905385002516354 22.01811776547559 10-11 34.09348620385536 27.126118180672798 17.903489983621014 20.876905631850825 12-13 33.82815448484086 21.2982765127689 21.93986664989307 22.93370235249717 14-15 32.1419550391513 24.021217479161404 22.265723667592827 21.57110381409447 16-17 33.99772927967705 22.770278793995207 21.60968840671124 21.6223035196165 18-19 33.38393421884883 24.52877925363694 20.948766603415557 21.138519924098674 20-21 33.185616611800455 24.9177006837174 21.233223600911625 20.663459103570524 22-23 33.977516736137424 24.478969306555516 21.46014904635594 20.08336491095112 24-25 32.75992916772072 24.373893245636225 21.237035163167214 21.62914242347584 26-27 31.618855048654115 25.274864147605207 22.317705042335398 20.78857576140528 28-29 26.139240506329113 30.949367088607595 21.949367088607595 20.962025316455694 30-31 28.29711975745326 27.918140474987368 22.51136937847398 21.273370389085397 32-33 24.126582278481013 26.430379746835442 28.70886075949367 20.734177215189874 34-35 26.480263157894733 25.708502024291498 27.062246963562753 20.748987854251013 36-37 31.248426881449788 26.038258243141204 21.910395167379814 20.802919708029197 38-39 25.27430949678396 29.701097237987135 23.420355656451004 21.604237608777904 40-41 25.15476942514214 28.46493998736576 25.369551484523058 21.010739102969044 42-43 26.081473073527555 26.396771345693026 26.295875898600073 21.22587968217934 44-45 26.331696259916885 25.802795617680392 27.099861478403227 20.765646643999496 46-47 25.403836446239275 26.67844522968198 27.41039878849066 20.50731953558809 48-49 24.146187775677376 26.528040327662257 28.56962822936358 20.756143667296787 50-51 23.484276729559745 30.100628930817606 25.459119496855347 20.955974842767294 52-53 26.7296786389414 30.17013232514178 23.276622558286075 19.82356647763075 54-55 27.59315206445116 27.467270896273916 24.773413897280967 20.166163141993955 56-57 29.974811083123427 25.264483627204033 23.803526448362717 20.957178841309823 58-59 31.42641129032258 25.529233870967744 23.664314516129032 19.380040322580644 60-61 31.83709494389106 24.7761946791073 22.355314588324298 21.031395788677344 62-63 32.58511979823455 25.182849936948298 21.9672131147541 20.264817150063053 64-65 31.443364061118828 24.599065538578103 22.805909837100643 21.151660563202423 66-67 29.81036662452592 26.86472819216182 22.54108723135272 20.783817951959545 68-69 23.96255060728745 31.591599190283397 24.202935222672064 20.242914979757085 70-71 23.75396322130628 30.602409638554217 25.656309448319593 19.98731769181991 72-73 22.635005744925316 32.46521128558662 24.549980850248946 20.349802119239115 74-75 21.733870967741936 31.93548387096774 23.978494623655912 22.352150537634408 76 27.264692885550158 0.0 36.14670790985418 36.58859920459567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 27.0 1 14.0 2 0.5 3 0.5 4 1.0 5 2.0 6 2.5 7 2.0 8 2.0 9 1.5 10 1.0 11 1.5 12 2.0 13 1.0 14 0.0 15 2.5 16 4.5 17 4.5 18 16.0 19 26.0 20 23.0 21 23.0 22 22.5 23 17.5 24 19.0 25 23.5 26 23.5 27 21.5 28 20.5 29 26.5 30 36.5 31 50.5 32 64.0 33 65.0 34 57.5 35 67.5 36 87.0 37 97.5 38 102.5 39 100.0 40 112.5 41 136.0 42 152.0 43 163.0 44 165.5 45 158.0 46 159.0 47 171.5 48 165.0 49 160.5 50 163.5 51 150.0 52 136.0 53 129.5 54 132.5 55 144.0 56 144.0 57 121.5 58 116.5 59 127.5 60 128.5 61 130.0 62 131.5 63 113.5 64 91.0 65 89.0 66 76.5 67 58.5 68 55.5 69 61.0 70 53.5 71 41.0 72 43.0 73 38.0 74 31.0 75 27.5 76 22.0 77 19.0 78 15.5 79 12.0 80 8.5 81 4.5 82 3.5 83 3.0 84 2.5 85 3.5 86 2.5 87 1.5 88 4.0 89 4.0 90 1.0 91 0.5 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 1.0 98 0.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7000000000000001 2 0.65 3 0.6 4 0.6 5 0.6 6 0.6 7 0.6 8 0.65 9 0.65 10-11 0.7875 12-13 0.6375 14-15 1.0250000000000001 16-17 0.9125 18-19 1.1875 20-21 1.275 22-23 1.0375 24-25 1.175 26-27 1.0875 28-29 1.25 30-31 1.05 32-33 1.25 34-35 1.2 36-37 0.07545271629778671 38-39 0.2892354124748491 40-41 0.46529175050301813 42-43 0.2892354124748491 44-45 0.13832997987927564 46-47 0.35211267605633806 48-49 0.2137826961770624 50-51 0.025150905432595575 52-53 0.2012325493648598 54-55 0.0629009938357026 56-57 0.03776910487221453 58-59 0.03778813452575891 60-61 0.0 62-63 0.0 64-65 0.03786922494319616 66-67 0.03791229622140781 68-69 0.0 70-71 0.0 72-73 0.10202780257620202 74-75 0.10741138560687433 76 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 35 24.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 1.0 53 0.0 54 1.0 55 2.0 56 1.0 57 1.0 58 1.0 59 3.0 60 1.0 61 0.0 62 0.0 63 3.0 64 2.0 65 2.0 66 3.0 67 2.0 68 2.0 69 6.0 70 5.0 71 7.0 72 25.0 73 79.0 74 210.0 75 1356.0 76 2263.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.175 #Duplication Level Percentage of deduplicated Percentage of total 1 95.70700295143547 89.17500000000001 2 3.058760397102227 5.7 3 0.778105715052321 2.175 4 0.053662463107056614 0.2 5 0.16098738932116982 0.75 6 0.08049369466058491 0.44999999999999996 7 0.08049369466058491 0.525 8 0.026831231553528307 0.2 9 0.026831231553528307 0.22499999999999998 >10 0.026831231553528307 0.6 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 24 0.6 No Hit CCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTT 9 0.22499999999999998 No Hit GGAACTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTGGCTTTAGAAGCCTGTGTACA 8 0.2 No Hit CTGTATTACAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGAAATGCA 7 0.17500000000000002 No Hit CTTTAGGACATCCTTGGGGAAATGCACCTGGTGCAGCAGCTAATCGAGTG 7 0.17500000000000002 No Hit AAATTATCCGAGCAGCTTGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCG 7 0.17500000000000002 No Hit GGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGATACTATCG 6 0.15 No Hit AGCTAATCGAGTGGCTTTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGA 6 0.15 No Hit CGATTAATAGATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAACA 6 0.15 No Hit TGCAAATGGAGTCCTGAACTAGCCGCAGCTTGTGAAGTATGGAAGGCGATCAAATTCGAGTTCGCGCCGGTAGAT 5 0.125 No Hit GATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAACAAAATAAAAAGAGAAAAAATAAGTTACGAAATGC 5 0.125 No Hit ATTAATAGATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAACAAAATAAAAAGAGAAAAAATAAGTTACG 5 0.125 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAA 5 0.125 No Hit ATCGATTAATAGATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAA 5 0.125 No Hit GATTAATAGATAAAACTAAATATGAAGAAGGTCTAAATAAAAAGAAACAA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 29 0.0 0.0 0.0 0.0 0.0 30 0.0 0.0 0.0 0.0 0.0 31 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 35 0.0 0.0 0.0 0.0 0.0 36 0.0 0.0 0.0 0.0 0.0 37 0.0 0.0 0.0 0.0 0.0 38 0.0 0.0 0.0 0.0 0.0 39 0.0 0.0 0.0 0.0 0.0 40 0.0 0.0 0.0 0.0 0.0 41 0.0 0.0 0.0 0.0 0.0 42 0.0 0.0 0.0 0.0 0.0 43 0.0 0.0 0.0 0.0 0.0 44 0.0 0.0 0.0 0.0 0.0 45 0.0 0.0 0.0 0.0 0.0 46 0.0 0.0 0.0 0.0 0.0 47 0.0 0.0 0.0 0.0 0.0 48 0.0 0.0 0.0 0.0 0.0 49 0.0 0.0 0.0 0.0 0.0 50 0.0 0.0 0.0 0.0 0.0 51 0.0 0.0 0.0 0.0 0.0 52 0.0 0.0 0.0 0.0 0.0 53 0.0 0.0 0.0 0.0 0.0 54 0.0 0.0 0.0 0.0 0.0 55 0.0 0.0 0.0 0.0 0.0 56 0.0 0.0 0.0 0.0 0.0 57 0.0 0.0 0.0 0.0 0.0 58 0.0 0.0 0.0 0.0 0.0 59 0.0 0.0 0.0 0.0 0.0 60 0.0 0.0 0.0 0.0 0.0 61 0.0 0.0 0.0 0.0 0.0 62 0.0 0.0 0.0 0.0 0.0 63 0.0 0.0 0.0 0.0 0.0 64 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTTGG 15 0.0021291415 69.43589 31 GGGGAGA 15 0.0021291415 69.43589 62 >>END_MODULE Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635741 spots for SRR11389805.sra Written 1635741 spots for SRR11389805.sra Read 1635755 spots for SRR11389805.sra Written 1635755 spots for SRR11389805.sra SRR ids: ['SRR11389805.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_18zft92s SRR11389805.sra spots: 32714834 blocks: [[1, 1635741], [1635742, 3271482], [3271483, 4907223], [4907224, 6542964], [6542965, 8178705], [8178706, 9814446], [9814447, 11450187], [11450188, 13085928], [13085929, 14721669], [14721670, 16357410], [16357411, 17993151], [17993152, 19628892], [19628893, 21264633], [21264634, 22900374], [22900375, 24536115], [24536116, 26171856], [26171857, 27807597], [27807598, 29443338], [29443339, 31079079], [31079080, 32714834]] SRR11389805 file size 6210021 SRR11389805 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11389805 SRR11389805_1.fastq SRR11389805_2.fastq Input file: SRR11389805_1.fastq Paired file: SRR11389805_2.fastq trimmed: SRR11389805-trimmed-pair1.fastq, SRR11389805-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 06:58:05 2024 >> started Sat Dec 7 06:58:31 2024 >> done (25.458s) 32714834 read pairs processed; of these: 598 ( 0.00%) short read pairs filtered out after trimming by size control 4066631 (12.43%) empty read pairs filtered out after trimming by size control 28647605 (87.57%) read pairs available; of these: 62673 ( 0.22%) trimmed read pairs available after processing 28584932 (99.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 638 0.00% 19 59 0.00% 20 894 0.00% 21 65 0.00% 22 1146 0.00% 23 46 0.00% 24 1395 0.00% 25 61 0.00% 26 1486 0.01% 27 46 0.00% 28 1562 0.01% 29 48 0.00% 30 1081 0.00% 31 36 0.00% 32 783 0.00% 33 23 0.00% 34 614 0.00% 35 415 0.00% 36 1775 0.01% 37 520 0.00% 38 1193 0.00% 39 679 0.00% 40 1156 0.00% 41 1081 0.00% 42 1341 0.00% 43 1351 0.00% 44 1773 0.01% 45 2098 0.01% 46 2296 0.01% 47 2620 0.01% 48 2888 0.01% 49 3337 0.01% 50 3730 0.01% 51 4355 0.02% 52 5156 0.02% 53 5750 0.02% 54 6358 0.02% 55 7236 0.03% 56 8058 0.03% 57 9183 0.03% 58 9948 0.03% 59 11163 0.04% 60 11686 0.04% 61 13059 0.05% 62 14643 0.05% 63 16249 0.06% 64 18121 0.06% 65 18763 0.07% 66 21239 0.07% 67 23225 0.08% 68 23464 0.08% 69 26451 0.09% 70 28339 0.10% 71 35848 0.13% 72 65327 0.23% 73 306573 1.07% 74 2179896 7.61% 75 13415314 46.83% 76 12323965 43.02% 28647605 reads passed initial QC criterion=sequence-density sequence-density=1.68 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=26 prefix-density=1.59 prefix-fanout=2.0 sequence=TTCAAATGTACA criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=53.16 fanout-score-rank=1 prefix-density=0.47 prefix-fanout=1.8 sequence=TTTCTTTTCAAGATTGCCACATCTCTTTTTGGGAGATGGTGGAATTTTCCCATCTTTTCTTCTGCTCTTAAGTCTCTAAATTGAGAAAGTCTAGTTTTCGTAATTGACCAATTCGTTAACATACCACTGAACCACTTTTTATTAACATAATGACAACGAGCCCTTATTGCAGCTGATGCTACTAAATCCGCTGCTCTTTTTTTGGTACCAACAATTAAGAAACTTTTTCCCTGACTTGCTGCATCAAAAACTAAATCACAAGCTTCTGATAAAAAACGAGCCGTTCTAGCGAGATTTATAATATGAGTACCTTTACGCTTTGCCGAAATGTAAGGGGCCATTTTAGGATTCCATTTCTTAATACCATGACCAAAATGAACTCCCGCTTCTATCATTTCTTTCAAATTGATGTTCCAATATCTTCTTGTCATTTTTTCCACACTTCCTTTTTATT criterion=sequence-density sequence-density=0.74 sequence-density-rank=1 fanout-score=2.26 fanout-score-rank=24 prefix-density=0.76 prefix-fanout=2.2 sequence=CAGGTGCTCAAGGAGCTGGAGGAGGTCAAGAAGGAGTACCCGGACGCCTA criterion=fanout-score sequence-density=0.02 sequence-density-rank=29 fanout-score=41.97 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=2.5 sequence=CGGCGAGCGCGATGCTTGCGGGCGGCGCCATGGCGCAGGACGTGCTGCTGGGTGCCAACGGCGGCGTGCTGGTGTTCGAGCCGAACGAGTTCAGCGTCAAGGCCGGGGAGACGATCACGTTCAAGAACAACGCCGGGTTCCCCCACAACATCGTGTTCGACGAGGACGCCGTGCCCAGCGGCGTCGACGTCTCCAAGATCTCCCAGGAGGAGTACCTCAACGCCCCCGGCGAGACTTTCTCCGTCACGCTCACTGTCCCTGGCACCTACGGCTTCTACTGCGAGCCACATGCCGGGGCCGGCATGGTCGGCAAGGTCACCGTCAACTGATTGATGCATCGCCCGGCCCGCCTTAATTTCTCCGTTTCAAGGGTGTCAATATATGATGATGTGTTGTTATAATGTACGCGCCTGCAAACTATATACATGCAGGATCATTGATGAGCCAGCTGATACTATATATTTCTCCATCTCTGTGAGTCATATGCT SRR11389805 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 07:00:13 Started mapping on | Dec 07 07:00:14 Finished on | Dec 07 07:04:00 Mapping speed, Million of reads per hour | 456.33 Number of input reads | 28647605 Average input read length | 150 UNIQUE READS: Uniquely mapped reads number | 20743607 Uniquely mapped reads % | 72.41% Average mapped length | 150.05 Number of splices: Total | 5615045 Number of splices: Annotated (sjdb) | 5246820 Number of splices: GT/AG | 5538113 Number of splices: GC/AG | 62307 Number of splices: AT/AC | 1194 Number of splices: Non-canonical | 13431 Mismatch rate per base, % | 0.56% Deletion rate per base | 0.01% Deletion average length | 1.60 Insertion rate per base | 0.01% Insertion average length | 1.55 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 5475198 % of reads mapped to multiple loci | 19.11% Number of reads mapped to too many loci | 258340 % of reads mapped to too many loci | 0.90% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.46% % of reads unmapped: other | 3.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2428800 2428800 2428800 N_multimapping 5475198 5475198 5475198 N_noFeature 1106145 19961502 1368864 N_ambiguous 869574 7164 427954 UnstrandedReadsAssigned:18767888 PositiveStrandReadsAssigned:774941 NegativeStrandReadsAssigned:18946789 Dataset is classified negative stranded MeadianReadLen=76 20thPercentileLength=75 echo kmer=71 SRR11389805 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR11389805-trimmed-pair1.fastq SRR11389805-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 28,647,605 reads, 23,487,574 reads pseudoaligned [quant] estimated average fragment length: 174.188 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,173 rounds 52973 SRR11389805.ke.tsv 35125 SRR11389805.se.tsv 88098 total ==> SRR11389805.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 762.9 0 0 PNS24247 1044 870.812 12.0791 0.777222 PNS24249 1928 1754.81 30.2893 0.967152 PNS24246 1044 870.812 12.0791 0.777222 PNS24248 1044 870.812 12.0791 0.777222 PNS24244 1471 1297.81 201.474 8.69847 PNS24243 293 130.28 0 0 KQK14069 1603 1429.81 1656.19 64.9036 KQK14071 474 302.2 14.8554 2.75439 ==> SRR11389805.se.tsv <== BRADI_1g14170v3 1838 BRADI_1g53295v3 8 BRADI_1g59795v3 252 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 197 BRADI_1g74790v3 210 BRADI_1g09890v3 0 BRADI_1g77505v3 178 BRADI_1g48960v3 0 SRR11389805 completed mapping pipeline successfully