Starting /dee2/code/volunteer_pipeline.sh SRR12666289 current disk space = 1543303016448 free memory = 1598698412 SRR12666289 SRAfilesize 4ffc93890cccb66d357843d1825e4f25 SRR12666289.sra SRR12666289.sra file validated SRR12666289 is paired end SRR12666289 is conventional basespace SRR12666289 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR12666289_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4665 37.0 37.0 37.0 37.0 37.0 2 36.18875 37.0 37.0 37.0 37.0 37.0 3 36.4515 37.0 37.0 37.0 37.0 37.0 4 36.557 37.0 37.0 37.0 37.0 37.0 5 36.6205 37.0 37.0 37.0 37.0 37.0 6 36.5335 37.0 37.0 37.0 37.0 37.0 7 36.425 37.0 37.0 37.0 37.0 37.0 8 36.5595 37.0 37.0 37.0 37.0 37.0 9 36.5675 37.0 37.0 37.0 37.0 37.0 10-14 36.530699999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.551300000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.5509 37.0 37.0 37.0 37.0 37.0 25-29 36.47859999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.3789 37.0 37.0 37.0 37.0 37.0 35-39 36.4614 37.0 37.0 37.0 37.0 37.0 40-44 36.382799999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.327600000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.3318 37.0 37.0 37.0 37.0 37.0 55-59 36.3224 37.0 37.0 37.0 37.0 37.0 60-64 36.3241 37.0 37.0 37.0 37.0 37.0 65-69 36.2435 37.0 37.0 37.0 37.0 37.0 70-74 36.226600000000005 37.0 37.0 37.0 37.0 37.0 75-79 36.2135 37.0 37.0 37.0 37.0 37.0 80-84 36.1813 37.0 37.0 37.0 37.0 37.0 85-89 36.18309999999999 37.0 37.0 37.0 37.0 37.0 90-94 36.144099999999995 37.0 37.0 37.0 37.0 37.0 95-99 36.109300000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.1596 37.0 37.0 37.0 37.0 37.0 105-109 36.13289999999999 37.0 37.0 37.0 37.0 37.0 110-114 36.0308 37.0 37.0 37.0 37.0 37.0 115-119 35.9197 37.0 37.0 37.0 37.0 37.0 120-124 35.9271 37.0 37.0 37.0 37.0 37.0 125-129 35.9827 37.0 37.0 37.0 37.0 37.0 130-134 35.857099999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.8394 37.0 37.0 37.0 37.0 37.0 140-144 35.714 37.0 37.0 37.0 37.0 37.0 145-149 35.579600000000006 37.0 37.0 37.0 37.0 37.0 150-151 35.3575 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 2.0 24 1.0 25 3.0 26 8.0 27 5.0 28 12.0 29 23.0 30 22.0 31 47.0 32 83.0 33 98.0 34 141.0 35 296.0 36 2774.0 37 484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.85 13.15 8.025 30.975 2 21.777221526908637 16.420525657071337 36.520650813516895 25.281602002503128 3 20.549999999999997 24.825 25.900000000000002 28.725 4 26.200000000000003 30.099999999999998 20.150000000000002 23.549999999999997 5 24.175 33.074999999999996 21.45 21.3 6 22.2 33.1 23.05 21.65 7 17.825 19.825 40.849999999999994 21.5 8 19.675 19.5 28.4 32.425 9 20.775 18.8 30.85 29.575000000000003 10-14 23.59 25.465 23.96 26.985 15-19 23.435 24.445 25.255 26.865 20-24 23.515 25.679999999999996 24.555 26.25 25-29 23.07 23.96 25.685000000000002 27.284999999999997 30-34 24.145 24.169999999999998 25.335 26.35 35-39 23.175 25.06 24.85 26.915 40-44 23.965 25.03 25.490000000000002 25.515 45-49 23.32 24.285 25.259999999999998 27.134999999999998 50-54 23.05 25.66 25.28 26.009999999999998 55-59 23.54 25.174999999999997 24.529999999999998 26.755000000000003 60-64 23.119999999999997 24.709999999999997 26.035000000000004 26.135 65-69 22.58 25.374999999999996 24.490000000000002 27.555000000000003 70-74 23.77 25.14 24.84 26.25 75-79 24.21 24.349999999999998 24.595 26.845000000000002 80-84 22.720000000000002 24.945 24.88 27.455000000000002 85-89 23.69 24.98 24.83 26.5 90-94 23.165 23.71 25.515 27.61 95-99 23.150000000000002 24.8 24.740000000000002 27.310000000000002 100-104 23.74 24.82 24.755 26.685 105-109 23.35 25.3 25.480000000000004 25.869999999999997 110-114 22.71 25.635 24.77 26.884999999999998 115-119 24.745 24.575 24.05 26.63 120-124 24.529999999999998 24.52 24.685000000000002 26.265 125-129 24.59 24.29 23.68 27.439999999999998 130-134 24.48 24.3 24.515 26.705000000000002 135-139 23.97 25.019999999999996 23.97 27.04 140-144 24.095 24.65 24.41 26.845000000000002 145-149 24.41 24.03 24.325 27.235 150-151 24.025 24.462500000000002 25.1 26.4125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 1.0 27 0.5 28 0.5 29 2.5 30 6.0 31 7.0 32 8.0 33 11.5 34 17.5 35 28.5 36 38.0 37 40.5 38 47.0 39 75.5 40 93.0 41 104.5 42 125.0 43 154.5 44 172.0 45 168.5 46 185.0 47 195.0 48 188.0 49 200.5 50 216.5 51 217.5 52 195.0 53 172.5 54 152.5 55 140.5 56 127.5 57 111.5 58 120.0 59 99.5 60 78.5 61 66.5 62 56.5 63 60.5 64 42.5 65 35.5 66 38.5 67 33.5 68 35.0 69 28.0 70 22.0 71 21.0 72 20.0 73 13.0 74 6.5 75 6.5 76 5.5 77 3.0 78 1.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.75 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42201834862385 70.65 2 9.724770642201836 15.9 3 1.9571865443425076 4.8 4 0.7033639143730887 2.3 5 0.581039755351682 2.375 6 0.24464831804281345 1.2 7 0.06116207951070336 0.35000000000000003 8 0.12232415902140673 0.8 9 0.06116207951070336 0.44999999999999996 >10 0.12232415902140673 1.175 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 13 0.325 No Hit GCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGTCAGA 12 0.3 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 12 0.3 No Hit GTAGCTTCGCGCCACTGGCTTTTCAACCAAGCGCGATGACCAATTGTGTG 10 0.25 No Hit GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCC 9 0.22499999999999998 No Hit CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG 9 0.22499999999999998 No Hit GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC 8 0.2 No Hit GCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAG 8 0.2 No Hit GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 8 0.2 No Hit GGCAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTT 8 0.2 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 7 0.17500000000000002 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 7 0.17500000000000002 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 6 0.15 No Hit GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCG 6 0.15 No Hit GCCGACTTCCCTTGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTT 6 0.15 No Hit GTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTC 6 0.15 No Hit AGAACTCGTAATGGGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC 6 0.15 No Hit GGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGA 6 0.15 No Hit CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 6 0.15 No Hit GTTCAGTCATAATCCGGCACACGGTAGCTTCGCGCCACTGGCTTTTCAAC 6 0.15 No Hit GTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTT 5 0.125 No Hit GTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAGCGGATTTCGACTTC 5 0.125 No Hit CTCCTACTCATCGGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCG 5 0.125 No Hit CTCGTAATGGGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT 5 0.125 No Hit CCAGTCTTTTGCCTAGCACACTAAAATTGGGTTCTGCCCGTAGAGAAGCA 5 0.125 No Hit GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAG 5 0.125 No Hit GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG 5 0.125 No Hit CTTGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGA 5 0.125 No Hit GGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC 5 0.125 No Hit CTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGG 5 0.125 No Hit ACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGACTTCTCGCGACG 5 0.125 No Hit GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA 5 0.125 No Hit GTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCAT 5 0.125 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 5 0.125 No Hit GCGACGTGGGGCTGGATCTCAGTGGATCGTGGCAGCAAGGCCACTCTGCC 5 0.125 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 5 0.125 No Hit GTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTT 5 0.125 No Hit GAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTATCTAATAAA 5 0.125 No Hit GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2375 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4 0.0 0.0 0.0 0.0 90-91 0.45 0.0 0.0 0.0 0.0 92-93 0.5 0.0 0.0 0.0 0.0 94-95 0.575 0.0 0.0 0.0 0.0 96-97 0.7125 0.0 0.0 0.0 0.0 98-99 0.8999999999999999 0.0 0.0 0.0 0.0 100-101 1.0750000000000002 0.0 0.0 0.0 0.0 102-103 1.15 0.0 0.0 0.0 0.0 104-105 1.2875 0.0 0.0 0.0 0.0 106-107 1.475 0.0 0.0 0.0 0.0 108-109 1.625 0.0 0.0 0.0 0.0 110-111 1.7000000000000002 0.0 0.0 0.0 0.0 112-113 1.95 0.0 0.0 0.0 0.0 114-115 2.2375 0.0 0.0 0.0 0.0 116-117 2.575 0.0 0.0 0.0 0.0 118-119 2.9 0.0 0.0 0.0 0.0 120-121 3.3125 0.0 0.0 0.0 0.0 122-123 3.925 0.0 0.0 0.0 0.0 124-125 4.275 0.0 0.0 0.0 0.0 126-127 4.487500000000001 0.0 0.0 0.0 0.0 128-129 4.8375 0.0 0.0 0.0 0.0 130-131 5.2875 0.0 0.0 0.0 0.0 132-133 5.775 0.0 0.0 0.0 0.0 134-135 6.1875 0.0 0.0 0.0 0.0 136-137 6.55 0.0 0.0 0.0 0.0 138-139 7.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAACAA 10 0.006830828 145.0 1 >>END_MODULE SRR12666289 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR12666289_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.996 37.0 37.0 37.0 37.0 37.0 2 35.9345 37.0 37.0 37.0 37.0 37.0 3 35.9925 37.0 37.0 37.0 37.0 37.0 4 36.1075 37.0 37.0 37.0 37.0 37.0 5 36.0965 37.0 37.0 37.0 37.0 37.0 6 35.887 37.0 37.0 37.0 37.0 37.0 7 36.063 37.0 37.0 37.0 37.0 37.0 8 36.0385 37.0 37.0 37.0 37.0 37.0 9 36.082 37.0 37.0 37.0 37.0 37.0 10-14 35.972300000000004 37.0 37.0 37.0 37.0 37.0 15-19 35.9803 37.0 37.0 37.0 37.0 37.0 20-24 35.9582 37.0 37.0 37.0 37.0 37.0 25-29 35.8429 37.0 37.0 37.0 37.0 37.0 30-34 35.8052 37.0 37.0 37.0 37.0 37.0 35-39 35.7782 37.0 37.0 37.0 37.0 37.0 40-44 35.7577 37.0 37.0 37.0 37.0 37.0 45-49 35.70960000000001 37.0 37.0 37.0 37.0 37.0 50-54 35.6574 37.0 37.0 37.0 37.0 37.0 55-59 35.733 37.0 37.0 37.0 37.0 37.0 60-64 35.636300000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.646 37.0 37.0 37.0 37.0 37.0 70-74 35.622699999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.5963 37.0 37.0 37.0 37.0 37.0 80-84 35.5886 37.0 37.0 37.0 37.0 37.0 85-89 35.5294 37.0 37.0 37.0 37.0 37.0 90-94 35.5022 37.0 37.0 37.0 37.0 37.0 95-99 35.5268 37.0 37.0 37.0 37.0 37.0 100-104 35.4983 37.0 37.0 37.0 37.0 37.0 105-109 35.469899999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.4366 37.0 37.0 37.0 37.0 37.0 115-119 35.4186 37.0 37.0 37.0 37.0 37.0 120-124 35.403299999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.386900000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.349599999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.2172 37.0 37.0 37.0 34.6 37.0 140-144 35.04639999999999 37.0 37.0 37.0 29.8 37.0 145-149 35.0176 37.0 37.0 37.0 27.4 37.0 150-151 34.71225 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 6.0 13 13.0 14 12.0 15 8.0 16 9.0 17 3.0 18 7.0 19 4.0 20 7.0 21 8.0 22 5.0 23 12.0 24 10.0 25 10.0 26 11.0 27 10.0 28 11.0 29 19.0 30 36.0 31 37.0 32 59.0 33 95.0 34 178.0 35 463.0 36 2581.0 37 385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.225 15.950000000000001 7.449999999999999 25.374999999999996 2 30.25 19.525000000000002 28.95 21.275 3 26.474999999999998 21.275 27.925 24.325 4 29.65 30.625000000000004 19.325 20.4 5 30.625000000000004 33.575 17.474999999999998 18.325 6 25.275 34.325 19.575 20.825 7 23.575 17.325 33.975 25.124999999999996 8 24.15 21.125 22.6 32.125 9 26.3 21.25 25.074999999999996 27.375 10-14 27.91 25.215 22.015 24.86 15-19 28.395 23.96 23.43 24.215 20-24 27.634999999999998 25.245 23.575 23.544999999999998 25-29 27.79 24.315 24.115000000000002 23.78 30-34 26.845000000000002 25.650000000000002 23.775 23.73 35-39 27.145000000000003 24.9 23.775 24.18 40-44 27.3 25.365 23.93 23.405 45-49 27.525 25.055 23.77 23.65 50-54 26.900000000000002 25.040000000000003 24.310000000000002 23.75 55-59 27.105 25.22 23.84 23.835 60-64 27.689999999999998 25.045 23.24 24.025 65-69 27.49 24.51 24.14 23.86 70-74 26.595000000000002 25.080000000000002 23.985 24.34 75-79 27.93 24.545 24.169999999999998 23.355 80-84 27.229999999999997 24.14 24.985 23.645 85-89 27.089999999999996 25.3 23.845 23.765 90-94 27.125 25.56 24.265 23.05 95-99 28.544999999999998 24.490000000000002 23.885 23.080000000000002 100-104 26.765 25.295 24.915000000000003 23.025000000000002 105-109 27.825 24.84 24.395 22.939999999999998 110-114 27.889999999999997 25.779999999999998 23.315 23.015 115-119 28.13 25.53 23.52 22.82 120-124 27.63 25.074999999999996 23.905 23.39 125-129 27.744999999999997 25.380000000000003 23.235 23.64 130-134 28.275 25.6 23.09 23.035 135-139 27.665 26.16 23.715 22.46 140-144 28.595 25.88 22.689999999999998 22.835 145-149 28.555000000000003 26.565 22.720000000000002 22.16 150-151 30.349999999999998 24.8 23.400000000000002 21.45 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 1.0 10 1.0 11 0.0 12 0.5 13 0.5 14 0.5 15 1.0 16 0.5 17 0.0 18 1.0 19 2.0 20 3.0 21 2.0 22 0.5 23 2.0 24 2.0 25 0.5 26 2.0 27 3.5 28 2.5 29 2.0 30 2.0 31 3.5 32 6.5 33 11.0 34 15.5 35 26.5 36 34.0 37 34.0 38 47.0 39 67.5 40 81.5 41 96.5 42 113.0 43 133.5 44 154.5 45 156.0 46 165.5 47 196.5 48 210.0 49 201.0 50 194.5 51 170.0 52 155.0 53 180.0 54 163.0 55 132.5 56 117.5 57 100.5 58 104.5 59 87.0 60 71.5 61 77.5 62 66.0 63 62.0 64 63.0 65 57.5 66 53.5 67 72.0 68 76.0 69 41.5 70 31.0 71 27.5 72 19.5 73 14.5 74 13.5 75 15.5 76 9.0 77 4.5 78 4.0 79 3.0 80 1.5 81 0.5 82 2.0 83 1.5 84 0.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 1.5 98 1.5 99 0.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.325 #Duplication Level Percentage of deduplicated Percentage of total 1 87.87878787878788 73.225 2 8.94089408940894 14.899999999999999 3 1.8601860186018602 4.65 4 0.6000600060006 2.0 5 0.21002100210021002 0.8750000000000001 6 0.18001800180018002 0.8999999999999999 7 0.09000900090009001 0.525 8 0.030003000300030006 0.2 9 0.09000900090009001 0.675 >10 0.12001200120012002 2.0500000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGG 39 0.975 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 17 0.42500000000000004 No Hit CAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTC 14 0.35000000000000003 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 12 0.3 No Hit GGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG 9 0.22499999999999998 No Hit ATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG 9 0.22499999999999998 No Hit GTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTAT 9 0.22499999999999998 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 8 0.2 No Hit GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC 7 0.17500000000000002 No Hit GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTA 7 0.17500000000000002 No Hit GTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGCCTAT 7 0.17500000000000002 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 6 0.15 No Hit GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGT 6 0.15 No Hit GCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAA 6 0.15 No Hit GCAGAATTCACCAAGTGTTGGATTGTTCACCCACCAATAGGGAACGTGAG 6 0.15 No Hit GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC 6 0.15 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 6 0.15 No Hit GTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGA 5 0.125 No Hit CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAAC 5 0.125 No Hit AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGA 5 0.125 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 5 0.125 No Hit GTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAG 5 0.125 No Hit GTAAATCAGAGGAGAGCATTCACTTTTGCACTTTCCCTTCCACCACTGGG 5 0.125 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2375 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4 0.0 0.0 0.0 0.0 90-91 0.4625 0.0 0.0 0.0 0.0 92-93 0.525 0.0 0.0 0.0 0.0 94-95 0.6 0.0 0.0 0.0 0.0 96-97 0.7375 0.0 0.0 0.0 0.0 98-99 0.925 0.0 0.0 0.0 0.0 100-101 1.0750000000000002 0.0 0.0 0.0 0.0 102-103 1.1625 0.0 0.0 0.0 0.0 104-105 1.3125 0.0 0.0 0.0 0.0 106-107 1.5 0.0 0.0 0.0 0.0 108-109 1.65 0.0 0.0 0.0 0.0 110-111 1.725 0.0 0.0 0.0 0.0 112-113 1.9749999999999999 0.0 0.0 0.0 0.0 114-115 2.2625 0.0 0.0 0.0 0.0 116-117 2.6125 0.0 0.0 0.0 0.0 118-119 2.95 0.0 0.0 0.0 0.0 120-121 3.375 0.0 0.0 0.0 0.0 122-123 4.0125 0.0 0.0 0.0 0.0 124-125 4.387499999999999 0.0 0.0 0.0 0.0 126-127 4.612500000000001 0.0 0.0 0.0 0.0 128-129 4.95 0.0 0.0 0.0 0.0 130-131 5.3875 0.0 0.0 0.0 0.0 132-133 5.887499999999999 0.0 0.0 0.0 0.0 134-135 6.324999999999999 0.0 0.0 0.0 0.0 136-137 6.7125 0.0 0.0 0.0 0.0 138-139 7.199999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAGCT 10 0.006830828 145.0 1 AGTTGGG 10 0.006830828 145.0 7 >>END_MODULE Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785012 spots for SRR12666289.sra Written 1785012 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra Read 1785000 spots for SRR12666289.sra Written 1785000 spots for SRR12666289.sra SRR ids: ['SRR12666289.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nd_etd9u SRR12666289.sra spots: 35700012 blocks: [[1, 1785000], [1785001, 3570000], [3570001, 5355000], [5355001, 7140000], [7140001, 8925000], [8925001, 10710000], [10710001, 12495000], [12495001, 14280000], [14280001, 16065000], [16065001, 17850000], [17850001, 19635000], [19635001, 21420000], [21420001, 23205000], [23205001, 24990000], [24990001, 26775000], [26775001, 28560000], [28560001, 30345000], [30345001, 32130000], [32130001, 33915000], [33915001, 35700012]] SRR12666289 file size 12110725 SRR12666289 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR12666289 SRR12666289_1.fastq SRR12666289_2.fastq Input file: SRR12666289_1.fastq Paired file: SRR12666289_2.fastq trimmed: SRR12666289-trimmed-pair1.fastq, SRR12666289-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 12:58:13 2024 >> started Sat Dec 7 12:58:53 2024 >> done (39.884s) 35700012 read pairs processed; of these: 89 ( 0.00%) short read pairs filtered out after trimming by size control 8516 ( 0.02%) empty read pairs filtered out after trimming by size control 35691407 (99.98%) read pairs available; of these: 3905921 (10.94%) trimmed read pairs available after processing 31785486 (89.06%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 7 0.00% 19 13 0.00% 20 18 0.00% 21 16 0.00% 22 17 0.00% 23 28 0.00% 24 23 0.00% 25 29 0.00% 26 19 0.00% 27 17 0.00% 28 41 0.00% 29 31 0.00% 30 44 0.00% 31 59 0.00% 32 50 0.00% 33 51 0.00% 34 34 0.00% 35 51 0.00% 36 57 0.00% 37 54 0.00% 38 78 0.00% 39 63 0.00% 40 73 0.00% 41 62 0.00% 42 82 0.00% 43 77 0.00% 44 86 0.00% 45 86 0.00% 46 105 0.00% 47 129 0.00% 48 133 0.00% 49 124 0.00% 50 184 0.00% 51 191 0.00% 52 196 0.00% 53 220 0.00% 54 244 0.00% 55 250 0.00% 56 257 0.00% 57 288 0.00% 58 345 0.00% 59 337 0.00% 60 370 0.00% 61 404 0.00% 62 538 0.00% 63 551 0.00% 64 630 0.00% 65 696 0.00% 66 737 0.00% 67 808 0.00% 68 938 0.00% 69 1041 0.00% 70 1235 0.00% 71 1318 0.00% 72 1550 0.00% 73 1892 0.01% 74 2035 0.01% 75 2203 0.01% 76 2381 0.01% 77 2496 0.01% 78 2919 0.01% 79 3523 0.01% 80 3895 0.01% 81 4594 0.01% 82 5217 0.01% 83 5916 0.02% 84 6645 0.02% 85 6742 0.02% 86 7918 0.02% 87 8537 0.02% 88 9019 0.03% 89 9928 0.03% 90 10658 0.03% 91 12610 0.04% 92 14254 0.04% 93 15824 0.04% 94 16320 0.05% 95 19875 0.06% 96 18937 0.05% 97 19424 0.05% 98 21113 0.06% 99 21961 0.06% 100 24041 0.07% 101 24479 0.07% 102 27602 0.08% 103 30204 0.08% 104 32513 0.09% 105 32955 0.09% 106 34985 0.10% 107 36521 0.10% 108 37335 0.10% 109 39519 0.11% 110 42147 0.12% 111 45880 0.13% 112 46970 0.13% 113 47047 0.13% 114 49469 0.14% 115 53473 0.15% 116 55597 0.16% 117 55061 0.15% 118 54852 0.15% 119 58252 0.16% 120 61787 0.17% 121 60889 0.17% 122 65592 0.18% 123 67432 0.19% 124 73695 0.21% 125 73742 0.21% 126 78784 0.22% 127 78975 0.22% 128 77162 0.22% 129 80932 0.23% 130 77479 0.22% 131 80151 0.22% 132 82212 0.23% 133 89344 0.25% 134 92988 0.26% 135 93282 0.26% 136 95469 0.27% 137 107147 0.30% 138 99769 0.28% 139 97341 0.27% 140 94486 0.26% 141 99860 0.28% 142 104472 0.29% 143 104194 0.29% 144 108621 0.30% 145 108997 0.31% 146 109788 0.31% 147 114835 0.32% 148 112794 0.32% 149 106766 0.30% 150 106113 0.30% 151 31785486 89.06% 35691407 reads passed initial QC criterion=sequence-density sequence-density=1.55 sequence-density-rank=1 fanout-score=2.76 fanout-score-rank=27 prefix-density=1.72 prefix-fanout=2.5 sequence=CCCAGCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGATCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTTGCCCCGCTTCCGACCCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=175.80 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=14.8 sequence=CGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGA criterion=sequence-density sequence-density=0.93 sequence-density-rank=1 fanout-score=2.60 fanout-score-rank=29 prefix-density=0.95 prefix-fanout=2.5 sequence=TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGTGCCGGATTATGACTGAACGCCTCTAAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGCGCCCGCCGCCTGCCCCGACCCACGTTAGGGGCGCTTGCGCCCCCAAGGGCACGTGCCATTGGCTAAGCCGTTCCGGCCGACGAGCCGCGTCCGGCCGCCTCGAAGCTCCCTTCCCCACGGGCGGCGGGCTGAATCCTTTGCAGACGACTTAAATACGCGACGGGGCATTGTAAGTGGCAGAGTGGCCTTGCTGCCACGATCCACTGAGATCCAGCCCCACGTCGCACG criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=303.75 fanout-score-rank=1 prefix-density=0.59 prefix-fanout=8.8 sequence=GCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGC SRR12666289 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 12:59:37 Started mapping on | Dec 07 12:59:37 Finished on | Dec 07 13:05:13 Mapping speed, Million of reads per hour | 382.41 Number of input reads | 35691407 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 21658658 Uniquely mapped reads % | 60.68% Average mapped length | 296.46 Number of splices: Total | 22613199 Number of splices: Annotated (sjdb) | 21267017 Number of splices: GT/AG | 22303002 Number of splices: GC/AG | 252040 Number of splices: AT/AC | 15607 Number of splices: Non-canonical | 42550 Mismatch rate per base, % | 0.26% Deletion rate per base | 0.02% Deletion average length | 2.25 Insertion rate per base | 0.01% Insertion average length | 1.99 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 319430 % of reads mapped to multiple loci | 0.89% Number of reads mapped to too many loci | 1440487 % of reads mapped to too many loci | 4.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.27% % of reads unmapped: other | 29.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 13713319 13713319 13713319 N_multimapping 319430 319430 319430 N_noFeature 851432 21115396 1026194 N_ambiguous 456298 3258 88614 UnstrandedReadsAssigned:20350928 PositiveStrandReadsAssigned:540004 NegativeStrandReadsAssigned:20543850 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR12666289 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR12666289-trimmed-pair1.fastq SRR12666289-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 35,691,407 reads, 21,200,956 reads pseudoaligned [quant] estimated average fragment length: 288.351 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,175 rounds 52973 SRR12666289.ke.tsv 35125 SRR12666289.se.tsv 88098 total ==> SRR12666289.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 650.107 0 0 PNS24247 1044 756.649 104.617 9.96829 PNS24249 1928 1640.65 103.313 4.53996 PNS24246 1044 756.649 104.617 9.96829 PNS24248 1044 756.649 104.617 9.96829 PNS24244 1471 1183.65 237.835 14.4866 PNS24243 293 93.6441 0 0 KQK14069 1603 1315.65 6627.19 363.163 KQK14071 474 226.045 96.2892 30.711 ==> SRR12666289.se.tsv <== BRADI_1g14170v3 7108 BRADI_1g53295v3 105 BRADI_1g59795v3 533 BRADI_1g07683v3 0 BRADI_1g00485v3 87 BRADI_1g20270v3 1348 BRADI_1g74790v3 53 BRADI_1g09890v3 0 BRADI_1g77505v3 144 BRADI_1g48960v3 1 SRR12666289 completed mapping pipeline successfully