Starting /dee2/code/volunteer_pipeline.sh SRR13165346 current disk space = 1542252937216 free memory = 1601710756 SRR13165346 SRAfilesize d523f39e1d7849cd5658a5b2b5c04ce7 SRR13165346.sra SRR13165346.sra file validated SRR13165346 is paired end SRR13165346 is conventional basespace SRR13165346 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165346_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4835 37.0 37.0 37.0 37.0 37.0 2 36.0495 37.0 37.0 37.0 37.0 37.0 3 36.5 37.0 37.0 37.0 37.0 37.0 4 36.5605 37.0 37.0 37.0 37.0 37.0 5 36.596 37.0 37.0 37.0 37.0 37.0 6 36.5455 37.0 37.0 37.0 37.0 37.0 7 36.4845 37.0 37.0 37.0 37.0 37.0 8 36.4975 37.0 37.0 37.0 37.0 37.0 9 36.4925 37.0 37.0 37.0 37.0 37.0 10-14 36.5292 37.0 37.0 37.0 37.0 37.0 15-19 36.5089 37.0 37.0 37.0 37.0 37.0 20-24 36.5573 37.0 37.0 37.0 37.0 37.0 25-29 36.4886 37.0 37.0 37.0 37.0 37.0 30-34 36.4419 37.0 37.0 37.0 37.0 37.0 35-39 36.4269 37.0 37.0 37.0 37.0 37.0 40-44 36.4144 37.0 37.0 37.0 37.0 37.0 45-49 36.3494 37.0 37.0 37.0 37.0 37.0 50-54 36.3766 37.0 37.0 37.0 37.0 37.0 55-59 36.361000000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.367200000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.353300000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.318400000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.315599999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.26090000000001 37.0 37.0 37.0 37.0 37.0 85-89 36.2151 37.0 37.0 37.0 37.0 37.0 90-94 36.162600000000005 37.0 37.0 37.0 37.0 37.0 95-99 36.191599999999994 37.0 37.0 37.0 37.0 37.0 100-104 36.125 37.0 37.0 37.0 37.0 37.0 105-109 36.1672 37.0 37.0 37.0 37.0 37.0 110-114 36.110400000000006 37.0 37.0 37.0 37.0 37.0 115-119 36.0739 37.0 37.0 37.0 37.0 37.0 120-124 35.9641 37.0 37.0 37.0 37.0 37.0 125-129 35.9563 37.0 37.0 37.0 37.0 37.0 130-134 35.98299999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.872299999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.8703 37.0 37.0 37.0 37.0 37.0 145-149 35.6553 37.0 37.0 37.0 37.0 37.0 150-151 35.57725 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 0.0 24 1.0 25 0.0 26 7.0 27 13.0 28 10.0 29 13.0 30 25.0 31 39.0 32 55.0 33 87.0 34 128.0 35 327.0 36 2832.0 37 461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.275 10.325 6.0249999999999995 39.375 2 23.521980798383023 11.268317331985852 32.364830722587165 32.84487114704396 3 21.7 12.625 24.425 41.25 4 27.85 18.875 21.275 32.0 5 29.175 26.900000000000002 21.25 22.675 6 25.7 28.475 21.9 23.925 7 19.400000000000002 25.674999999999997 36.9 18.025 8 21.875 24.8 27.400000000000002 25.924999999999997 9 21.275 20.849999999999998 32.5 25.374999999999996 10-14 23.14 25.805 26.405 24.65 15-19 23.815 24.185000000000002 25.564999999999998 26.435 20-24 24.310000000000002 24.095 25.41 26.185000000000002 25-29 24.275 24.055 25.064999999999998 26.605 30-34 24.044999999999998 24.610000000000003 25.069999999999997 26.275 35-39 24.92 23.155 25.145 26.779999999999998 40-44 25.235000000000003 23.71 25.040000000000003 26.015 45-49 24.44 24.01 25.355 26.195 50-54 24.41 24.2 25.25 26.14 55-59 24.77 24.169999999999998 24.645 26.415 60-64 25.259999999999998 23.630000000000003 24.815 26.295 65-69 24.625 25.314999999999998 23.915 26.145000000000003 70-74 25.88 23.94 23.995 26.185000000000002 75-79 25.424999999999997 24.104999999999997 24.445 26.025 80-84 24.67 24.855 24.07 26.405 85-89 24.755 23.905 24.975 26.365 90-94 24.455 23.655 25.240000000000002 26.650000000000002 95-99 24.485 24.195 24.709999999999997 26.61 100-104 25.66 23.599999999999998 24.73 26.009999999999998 105-109 25.245 24.41 24.05 26.295 110-114 25.56 24.65 23.494999999999997 26.295 115-119 25.0 24.545 23.64 26.815 120-124 25.435000000000002 24.055 24.065 26.445 125-129 25.205 24.065 24.215 26.515 130-134 25.46 24.955 23.57 26.015 135-139 25.435000000000002 24.095 24.240000000000002 26.229999999999997 140-144 25.85 23.974999999999998 24.104999999999997 26.07 145-149 24.41 24.529999999999998 24.705 26.355 150-151 25.75 23.2625 24.125 26.8625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.5 27 2.0 28 4.5 29 8.0 30 7.5 31 8.0 32 13.5 33 20.5 34 30.0 35 33.5 36 41.0 37 45.5 38 62.5 39 91.0 40 108.5 41 125.0 42 130.5 43 128.5 44 161.5 45 190.0 46 201.5 47 204.5 48 170.5 49 157.5 50 149.0 51 141.0 52 128.5 53 112.5 54 109.5 55 96.0 56 89.5 57 89.5 58 96.0 59 102.0 60 92.5 61 86.0 62 74.0 63 64.5 64 59.5 65 51.0 66 55.0 67 61.5 68 55.5 69 56.5 70 59.0 71 47.5 72 43.5 73 39.5 74 31.5 75 23.5 76 16.5 77 10.0 78 4.5 79 3.5 80 2.0 81 0.5 82 0.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.05 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.375 #Duplication Level Percentage of deduplicated Percentage of total 1 71.29798903107861 48.75 2 18.025594149908592 24.65 3 6.764168190127971 13.875000000000002 4 2.1206581352833638 5.800000000000001 5 1.0603290676416819 3.6249999999999996 6 0.43875685557586835 1.7999999999999998 7 0.14625228519195613 0.7000000000000001 8 0.14625228519195613 0.8 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGGAAAGCCACCAGTAGGTATCACTGGATGAGATGGTAAAAATGGTGCC 8 0.2 No Hit CACCATTATCCATACTGTTATATGCACCGCTGCCAAATTTTCCACCAATA 8 0.2 No Hit CCCGATTCCATAAGTAGCATCCAAAACTTTAAGCTTTTTCCTAAGTTCTT 8 0.2 No Hit AGGTAATGCAAACCACCACAAATCCCCCTAATTATTTTGTATCTCATGTT 8 0.2 No Hit GTGCTTTCTAACACAAGACCAAAGTCCTCAGCACCAGATGCAGTGTCTAT 7 0.17500000000000002 No Hit TTCGACAATTAATATGGATCGGAGGGAGTAATAGAAAACCAAAATACAAA 7 0.17500000000000002 No Hit GCTTATTACAAATTCACATCGAGCCATCCGGCATGCAGTACTGGAAAATA 7 0.17500000000000002 No Hit ATTCCTTTGTTTTAGTTGGAGAATGGATATGGAACACTGTATGGTGGAAG 7 0.17500000000000002 No Hit CTCTCTCTTTGCGCAGACCTGCTGTAATGCTCATGCAATGGACTCCCGCT 6 0.15 No Hit GACGACATCTTTGCTCCTCTTGATCTTCACAGACCGGGCATCCTTCCTCC 6 0.15 No Hit GGTGGCGAGCCAGCTACGTCACGCGGCTCTAGCGCAGTTGGAGCCGGAAT 6 0.15 No Hit TGGGCCAAAGCACCTGAGGACCCTCTTGACAAGGTCGCCATAGGCAAGGG 6 0.15 No Hit GCCTGGTGTTGTCGAAGCCGATGATGCGGACATAGGCGTCAGGGTACTCC 6 0.15 No Hit GGCCGGTACTCTCATTTGATCCAAATATCTTTAGAGTGAACACAAAATGG 6 0.15 No Hit CATTGGCAGTAAACGCAAAACCAGATAATTGCTCACCAAAGTACTCAACC 6 0.15 No Hit GGTCTCAAACATGATCTGGGTCATCTTCTCCCTGTTAGCCTTGGGGTTCA 6 0.15 No Hit CCTCGATCGGCCACACCTGCATGCAGCTGATCCTTCCGCCGTTGCTGACG 6 0.15 No Hit CCTTGATGATATCAGCAGTTTCCCATATGCTCAGGATACCATCTCTAGCA 6 0.15 No Hit AGCAGGGCTAAGCATATCAACAATCTGAGTTTGGCTCTCACTTTCCTGCA 6 0.15 No Hit GCGCAAGCCACGACCATGCGGGTCAGCAAAGACAGAAGCCTCGGAGAAGG 6 0.15 No Hit AGCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTT 5 0.125 No Hit CCCAGACAAATGGTTAAACAAGTGAGAACTACCCCAAATGTACGACAGTC 5 0.125 No Hit CCCTGCGGTTGTTCCTGAAGGCGCAGCAGCCGACGGAGTAGACAATGACG 5 0.125 No Hit AGTCAATACCCTTCGGCCTGCGCCAGCTAGTCTTGAGGCCGATGTAGCGG 5 0.125 No Hit GTTGGTTTTGGTCCTAACTTGTTCAAGTCATTATCTGGGTAACCGATATC 5 0.125 No Hit CATGAATGAATAAGAACAACGCTGTGAGTGAGAACAGTTTCGCCATTGCT 5 0.125 No Hit CCCACTAGTTTGTGTGGCTTCAGTTGATACCTTATCTTCCACAGGATTAG 5 0.125 No Hit GTCCGGTTGGTCTCGATGATGACATGCATGTCAAATTCTTTGGTGTTCAG 5 0.125 No Hit CCTCCATTGGTTTGTCTCGCCGTTATTTGCAAATCGATCATCCTTCCTGC 5 0.125 No Hit GGCAGCAACCCACGATCACACCGACGCCCACCACGTCGCCGGCCGAGTAC 5 0.125 No Hit GTCCACACCTCCACAATGGTATCAGCAGGAGGGTCACGTTCTGATGGTAT 5 0.125 No Hit GCTCAAACCAGATTAAGCCAATCAGAAAGGGCAATCACAGAGTCAATAGA 5 0.125 No Hit GTCGCATTTCAAGTCTCTGTGGATGATTGGTGGGTCATGGCTGTGGAGGT 5 0.125 No Hit CCACGGAGCGCAGGGGCCACGAACGTAGCCTCCTCGTCCTCCTCGGACAC 5 0.125 No Hit CCCCGTTCAGATGAGGGGCCAAACTCTCCTCACACCCACTCTCATCAGGC 5 0.125 No Hit CTCAAGTATAACTGACACCCAGATGTGTTGTCGATTGATATTGTTGGTGC 5 0.125 No Hit TGTGTGTGTATCTGAGTGGTGTCCTGTACTCCGCAGTTGGTGATTCTGAA 5 0.125 No Hit GCACCGTCATCGGCTTGCTGCTCGGGTACGGCAGGTCGTCGTACTTCTTG 5 0.125 No Hit CTCCAGCATCTCCTTGCCCACCGTCTCCAGCGTGTCACAGAAGGCCGGGT 5 0.125 No Hit ATATGGATGTATATATGTCTAAAAAATGTCTAGATATATGTAATAGAAAG 5 0.125 No Hit GTGCTGGAACAGGTTGTCGAGCGGCCCTTTTCCGGTGACCGCGTGCTGCA 5 0.125 No Hit AGCGCTGTTCCATTAACTTTGTCGGCTATAGTTTCCCCGATCATGATATC 5 0.125 No Hit GCCCTATGGAACTGTAACAGGAGATGCACCGTTGGTCGGTTCACTCGTCC 5 0.125 No Hit CAGTAACTGGTCCATCTTTGATAACATGCGTATGAGCCACCACAGCAATA 5 0.125 No Hit CGTCTCGATGATGACATGCATGTCAAATTCTTTGGTGTTCAGACCAATTC 5 0.125 No Hit TGGGGCGCTTGGTCATGCTCTGAGCAATCTTGGACCAGAAGACTGTCATG 5 0.125 No Hit GCCACTGGGACAATGATGCTCGAGGAAGCTATGGTTGCTACACCATAGGC 5 0.125 No Hit GTCATATCCGAGGAGGACGGAGTTCATGGAGGCGAGGAGCGCGCAGGCGA 5 0.125 No Hit AGCGCACATAAAAAATCTACTCTTGCCAGTTGATACATGCAGTCTACCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0125 12-13 0.0 0.0 0.0 0.0 0.025 14-15 0.0 0.0 0.0 0.0 0.025 16-17 0.0 0.0 0.0 0.0 0.025 18-19 0.0 0.0 0.0 0.0 0.025 20-21 0.0 0.0 0.0 0.0 0.025 22-23 0.0 0.0 0.0 0.0 0.025 24-25 0.0 0.0 0.0 0.0 0.025 26-27 0.0 0.0 0.0 0.0 0.025 28-29 0.0 0.0 0.0 0.0 0.025 30-31 0.0 0.0 0.0 0.0 0.025 32-33 0.0 0.0 0.0 0.0 0.025 34-35 0.0 0.0 0.0 0.0 0.025 36-37 0.025 0.0 0.0 0.0 0.025 38-39 0.025 0.0 0.0 0.0 0.025 40-41 0.05 0.0 0.0 0.0 0.025 42-43 0.05 0.0 0.0 0.0 0.025 44-45 0.05 0.0 0.0 0.0 0.025 46-47 0.05 0.0 0.0 0.0 0.025 48-49 0.05 0.0 0.0 0.0 0.025 50-51 0.05 0.0 0.0 0.0 0.025 52-53 0.05 0.0 0.0 0.0 0.025 54-55 0.05 0.0 0.0 0.0 0.025 56-57 0.05 0.0 0.0 0.0 0.025 58-59 0.0625 0.0 0.0 0.0 0.025 60-61 0.075 0.0 0.0 0.0 0.025 62-63 0.0875 0.0 0.0 0.0 0.025 64-65 0.1 0.0 0.0 0.0 0.025 66-67 0.1375 0.0 0.0 0.0 0.025 68-69 0.15 0.0 0.0 0.0 0.025 70-71 0.15 0.0 0.0 0.0 0.025 72-73 0.15 0.0 0.0 0.0 0.025 74-75 0.175 0.0 0.0 0.0 0.025 76-77 0.23750000000000002 0.0 0.0 0.0 0.025 78-79 0.3125 0.0 0.0 0.0 0.025 80-81 0.475 0.0 0.0 0.0 0.025 82-83 0.55 0.0 0.0 0.0 0.025 84-85 0.65 0.0 0.0 0.0 0.025 86-87 0.8 0.0 0.0 0.0 0.025 88-89 1.0 0.0 0.0 0.0 0.025 90-91 1.2875 0.0 0.0 0.0 0.025 92-93 1.3875 0.0 0.0 0.0 0.025 94-95 1.575 0.0 0.0 0.0 0.025 96-97 1.8875 0.0 0.0 0.0 0.025 98-99 2.25 0.0 0.0 0.0 0.025 100-101 2.4625 0.0 0.0 0.0 0.025 102-103 2.7 0.0 0.0 0.0 0.025 104-105 2.825 0.0 0.0 0.0 0.025 106-107 3.275 0.0 0.0 0.0 0.025 108-109 3.6500000000000004 0.0 0.0 0.0 0.025 110-111 4.025 0.0 0.0 0.0 0.025 112-113 4.425 0.0 0.0 0.0 0.025 114-115 4.800000000000001 0.0 0.0 0.0 0.025 116-117 5.4625 0.0 0.0 0.0 0.025 118-119 5.9125 0.0 0.0 0.0 0.025 120-121 6.324999999999999 0.0 0.0 0.0 0.025 122-123 7.125 0.0 0.0 0.0 0.025 124-125 7.949999999999999 0.0 0.0 0.0 0.025 126-127 8.6875 0.0 0.0 0.0 0.025 128-129 9.4625 0.0 0.0 0.0 0.025 130-131 9.8625 0.0 0.0 0.0 0.025 132-133 10.5125 0.0 0.0 0.0 0.025 134-135 11.1875 0.0 0.0 0.0 0.025 136-137 11.925 0.0 0.0 0.0 0.025 138-139 12.725 0.0 0.0 0.0 0.025 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACGG 40 0.005621335 54.375 145 >>END_MODULE SRR13165346 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165346_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9415 37.0 37.0 37.0 37.0 37.0 2 36.3475 37.0 37.0 37.0 37.0 37.0 3 36.2615 37.0 37.0 37.0 37.0 37.0 4 36.4005 37.0 37.0 37.0 37.0 37.0 5 36.383 37.0 37.0 37.0 37.0 37.0 6 36.2835 37.0 37.0 37.0 37.0 37.0 7 36.3935 37.0 37.0 37.0 37.0 37.0 8 36.392 37.0 37.0 37.0 37.0 37.0 9 36.378 37.0 37.0 37.0 37.0 37.0 10-14 36.3358 37.0 37.0 37.0 37.0 37.0 15-19 36.3475 37.0 37.0 37.0 37.0 37.0 20-24 36.2876 37.0 37.0 37.0 37.0 37.0 25-29 36.1892 37.0 37.0 37.0 37.0 37.0 30-34 36.206399999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.15509999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.1644 37.0 37.0 37.0 37.0 37.0 45-49 36.13955 37.0 37.0 37.0 37.0 37.0 50-54 36.164300000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.1447 37.0 37.0 37.0 37.0 37.0 60-64 36.0898 37.0 37.0 37.0 37.0 37.0 65-69 36.0801 37.0 37.0 37.0 37.0 37.0 70-74 36.0339 37.0 37.0 37.0 37.0 37.0 75-79 36.0673 37.0 37.0 37.0 37.0 37.0 80-84 35.98205 37.0 37.0 37.0 37.0 37.0 85-89 35.955799999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.963800000000006 37.0 37.0 37.0 37.0 37.0 95-99 35.8389 37.0 37.0 37.0 37.0 37.0 100-104 35.8787 37.0 37.0 37.0 37.0 37.0 105-109 35.806 37.0 37.0 37.0 37.0 37.0 110-114 35.857 37.0 37.0 37.0 37.0 37.0 115-119 35.7226 37.0 37.0 37.0 37.0 37.0 120-124 35.688900000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.53545 37.0 37.0 37.0 37.0 37.0 130-134 35.383799999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.3523 37.0 37.0 37.0 37.0 37.0 140-144 35.1723 37.0 37.0 37.0 32.2 37.0 145-149 34.9467 37.0 37.0 37.0 25.0 37.0 150-151 34.652249999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.0 15 0.0 16 2.0 17 1.0 18 0.0 19 2.0 20 2.0 21 1.0 22 4.0 23 2.0 24 3.0 25 4.0 26 7.0 27 10.0 28 21.0 29 17.0 30 28.0 31 38.0 32 71.0 33 124.0 34 200.0 35 493.0 36 2686.0 37 278.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.28463476070529 19.47103274559194 7.83375314861461 31.410579345088163 2 29.25 21.349999999999998 28.95 20.45 3 22.575 23.400000000000002 27.675 26.35 4 24.725 30.575000000000003 20.225 24.474999999999998 5 28.125 33.650000000000006 16.8 21.425 6 21.05 36.425000000000004 19.525000000000002 23.0 7 22.425 18.925 32.95 25.7 8 22.575 22.7 25.674999999999997 29.049999999999997 9 23.1 21.375 26.075 29.45 10-14 25.645 25.395 22.62 26.340000000000003 15-19 26.38 24.654999999999998 23.54 25.424999999999997 20-24 25.560448358686948 25.740592473979184 22.788230584467577 25.910728582866295 25-29 25.615615615615617 25.325325325325327 22.78778778778779 26.271271271271274 30-34 25.97838054248824 23.93153838454609 24.356921229106195 25.73315984385947 35-39 26.306044835868697 24.124299439551642 23.003402722177743 26.566253002401925 40-44 26.11850665599039 24.95245721149034 22.785506956260633 26.143529176258635 45-49 26.102186858830006 24.79607666516539 23.284792073262274 25.81694440274233 50-54 26.065852682145717 25.190152121697356 23.153522818254604 25.590472377902323 55-59 26.49149149149149 23.97897897897898 23.48848848848849 26.04104104104104 60-64 26.060848678943156 23.693955164131307 23.884107285828662 26.36108887109688 65-69 26.778745121585107 24.637246072250576 23.541479035324727 25.04252977083959 70-74 26.431431431431427 24.044044044044043 23.12812812812813 26.396396396396398 75-79 26.694024622159944 23.961565408867983 22.905615053548193 26.43879491542388 80-84 26.36477358018514 23.787840880660497 23.85789342006505 25.98949211908932 85-89 26.676676676676674 23.58858858858859 23.57857857857858 26.156156156156158 90-94 26.93654923939151 24.38951160928743 22.503002401921538 26.17093674939952 95-99 26.276020816653322 24.71477181745396 23.46377101681345 25.545436349079264 100-104 27.93793793793794 24.584584584584583 22.29229229229229 25.185185185185183 105-109 27.637637637637635 23.823823823823822 23.453453453453456 25.085085085085083 110-114 27.642113690952762 24.78482786228983 23.358686949559647 24.21437149719776 115-119 27.952952952952952 25.13013013013013 22.46246246246246 24.454454454454456 120-124 28.733733733733736 24.584584584584583 22.31231231231231 24.36936936936937 125-129 27.97657774886142 25.008758320404382 23.08693258595666 23.927731344777538 130-134 29.18918918918919 24.94994994994995 21.56156156156156 24.2992992992993 135-139 29.22922922922923 24.754754754754753 22.54254254254254 23.473473473473476 140-144 30.84967974379504 24.189351481184946 22.92333867093675 22.037630104083267 145-149 30.86086086086086 24.07907907907908 22.62762762762763 22.432432432432435 150-151 30.292792792792795 24.524524524524523 21.684184184184186 23.4984984984985 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 1.5 30 5.5 31 9.0 32 12.5 33 15.5 34 21.0 35 26.5 36 31.5 37 38.0 38 52.0 39 80.0 40 96.5 41 121.5 42 151.5 43 141.0 44 135.0 45 161.0 46 168.5 47 155.5 48 158.0 49 168.5 50 156.5 51 138.5 52 120.0 53 122.5 54 128.0 55 98.5 56 103.5 57 109.5 58 99.5 59 91.0 60 75.5 61 91.0 62 105.5 63 106.0 64 99.5 65 83.5 66 79.0 67 67.5 68 50.5 69 51.5 70 52.5 71 43.0 72 42.0 73 37.5 74 30.5 75 24.5 76 12.5 77 8.0 78 4.5 79 4.5 80 4.5 81 1.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.75 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.08 25-29 0.1 30-34 0.09 35-39 0.08 40-44 0.09 45-49 0.08499999999999999 50-54 0.08 55-59 0.1 60-64 0.08 65-69 0.06999999999999999 70-74 0.1 75-79 0.09 80-84 0.075 85-89 0.1 90-94 0.08 95-99 0.08 100-104 0.1 105-109 0.1 110-114 0.08 115-119 0.1 120-124 0.1 125-129 0.095 130-134 0.1 135-139 0.1 140-144 0.08 145-149 0.1 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.4 #Duplication Level Percentage of deduplicated Percentage of total 1 71.56432748538012 48.949999999999996 2 17.763157894736842 24.3 3 6.469298245614036 13.275 4 2.448830409356725 6.7 5 1.1330409356725146 3.875 6 0.3289473684210526 1.35 7 0.10964912280701754 0.525 8 0.14619883040935672 0.8 9 0.03654970760233918 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTTGACGGAGATGGAATGATCCATGCAATGCGTATTAAAGATGGGAAAG 9 0.22499999999999998 No Hit CTTTATTGTGTCAGTTACTAGGCTTGTCTCTAGCTTCAGATGACTGACAG 8 0.2 No Hit CCCAACTCTGACCTCCAGCAAGTGGTGGCCTGGTGGCTTAGGAAAAGCTT 8 0.2 No Hit CTCAGGTTGGGTTGACAAGCCCTGGTTTTATTGGAGCAGATGTTTGCCAT 8 0.2 No Hit GCCTCTTCTCGCTTGCTCTACCTGCTGCTTGCAACCATGGCACCCACCGT 8 0.2 No Hit GAGGCTCACATGGAAGTTCATGTTGGGTCAATTGACGAGGAAGAGGATGA 7 0.17500000000000002 No Hit GGCTCGTAATGCTCGCTTCAAGGCTGGGGGTAAATCTATTGTACTGGATG 7 0.17500000000000002 No Hit GATCATTGCGGCTAGAGTTAAAAGTTTTGATTATGTGGTTTGCGCTGTAG 7 0.17500000000000002 No Hit GTGCATGACTGGGCTGGACAAGAAGAAAGCCTCTGTCTTCTTCAAGACTT 6 0.15 No Hit TGCTACTGGTTGGGCTGGATTCTTTTGGGCGATTTGGTTTGCGGCAAGAA 6 0.15 No Hit CTTTCCGTGGCCGAAGCTACTCAAGGTCTCCATCGCCTCGGTACGAACGG 6 0.15 No Hit CAGAAAATAAATTGCTAGTGAAGCAAGCTCTCGATATCCTCATGCCTGCT 6 0.15 No Hit GCCACCCACCCGTCTCCTCCTCTTCCTCCGCTCTCCGCTCTCCGCTCTTA 6 0.15 No Hit GCCCTCCTCTCTGCTCCTTCTCCGGTGCTGTTTTCTGACTCAATTGCTCC 6 0.15 No Hit GCTGCTTTGAAGGGCTCTGACCACCGACGTGCTACCACTGTTTCTGCTAG 6 0.15 No Hit GTTAGAAATATATAATGAGACAATCCGTGACTTGTTAGCACCAGGCCGCT 6 0.15 No Hit CCGCCGTCGCGAGAAGACCTCGGCGACCCCCTCACCGCCAGGAAGATGCC 6 0.15 No Hit GAGGATTACACCTGTGTTGTTTCACATGGTCCTAACCCTAGAACTACACA 5 0.125 No Hit GTTGAGTACTTTGGTGAGCAATTATCTGGTTTTGCGTTTACTGCCAATGG 5 0.125 No Hit CCAAAACTTGAGCTTCAGATGGGTCGCAAATGGGTGGTTGAAAATCAAGT 5 0.125 No Hit AGGAAAGGTTACTGAAGCTTGCATTGTCAGAGGTGAACCAGAAACCGAAG 5 0.125 No Hit AATGTCTACAAGAACGTGAAGAAGAAGATTGAAAGGGGCATTGCTTTCCC 5 0.125 No Hit CCGTACGTGATGAACACCAACGTGTGGGCCAGCGGCGACGGCAAGAAGGA 5 0.125 No Hit CCTTACTTCCCTGGAGCCTCTGGATTACATTGTTGTTGCTTGTCTACCTG 5 0.125 No Hit GTCACAATCCTCAATTGGTCTTTTGTCAGGAACGACCAGCCAAGGTTTGA 5 0.125 No Hit AGAATATTCAATCAGTTTGCTGGGATTCCGTGGGTGACTATCTCGCTTCT 5 0.125 No Hit GTCAGGCACGACGCGCTGGGCCCGGAGCTCCGGTGGGCGCTGTTCGGCCG 5 0.125 No Hit GCTAGCAGCCTCTTCTCGCTTGCTCTACCTGCTGCTTGCAACCATGGCAC 5 0.125 No Hit GTATCAAGCACAAGAAGGTAGACATCAGAGCTCTAAAGAAATGGTCAAGG 5 0.125 No Hit GACGAGAACCGGCATGGGGATTTCTTCTCCGCGCTGCTTAAGGCGCAGCC 5 0.125 No Hit TGTAAAACTGCACATATTGCAGCTTCAAAAGTTGCTAAAGATGCCGGGGC 5 0.125 No Hit ATACGAAGCAAACAATGACAGTGTTACTGCTATTGGCCGTTATCTACTAC 5 0.125 No Hit GATCACTACAATATGTAATTCACAACTATGGCTTGGTTTATGTTGAACTT 5 0.125 No Hit CAAGGGGCTATCCGATTTCAACTTGGCGCTGGACCTCGGGTTCTTGACAA 5 0.125 No Hit AAACGATCTAGTGCAGCAGCAGCTTGCTCTCTCCTCCATCTAGTAGAAGA 5 0.125 No Hit GTAATGATTAATAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGG 5 0.125 No Hit GCCAAGCAGTTCCCGCGCCCCGCTAAGCCCTCCGCGGTCACCGGGCAAAA 5 0.125 No Hit CTAATTTCTCAGTTTCCTAATTAAGTTATGAATAGTTCCTGATATTAGTA 5 0.125 No Hit GCCGAGATTCTGGATGAACTCACAACAAACCGTGGAGAGCTAAAGCACAG 5 0.125 No Hit ATGTTCCCTGCTCCTTGGTTCCAGATCCGGGCGACGGTTGCTGCCTGACT 5 0.125 No Hit GGAAGGCGTCGCCAACGGAACCCTCAAGCTCGTGGGCGGCCACTACGACT 5 0.125 No Hit GTTAGGGTTAGGGTCATCCTCTGGAAGATCAGAGATGGCGGTGCCGCTGC 5 0.125 No Hit TGAGAAGTGAAGCTTGGCACGGCCACTCGCCAGAGACCTAACGTACAAAC 5 0.125 No Hit ACAACACCCCTTGATGTTCTGAAGACAAGGCTGATGGTTCAGGGGCAAAC 5 0.125 No Hit CTGCGGCATCTGCCACACGGACATCCACCAGGCCAAGAACCACCTTGGCG 5 0.125 No Hit GTACGACGCCGGCAAGTCCACATACTTCGCCTCCTCCTCCACGCTCTTCG 5 0.125 No Hit GTTGGATCAGGACAGGTCATACCAGCTTTTGAGGAGGCTATGACAGGCAT 5 0.125 No Hit CTTAAGCAGTGCAGAAGGTCTTACTTCAGATCCATCCATTGGAAAGCATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.0625 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.0875 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1375 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.23750000000000002 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.475 0.0 0.0 0.0 0.0 82-83 0.55 0.0 0.0 0.0 0.0 84-85 0.6375 0.0 0.0 0.0 0.0 86-87 0.775 0.0 0.0 0.0 0.0 88-89 0.975 0.0 0.0 0.0 0.0 90-91 1.2625 0.0 0.0 0.0 0.0 92-93 1.3625 0.0 0.0 0.0 0.0 94-95 1.55 0.0 0.0 0.0 0.0 96-97 1.8625 0.0 0.0 0.0 0.0 98-99 2.225 0.0 0.0 0.0 0.0 100-101 2.4375 0.0 0.0 0.0 0.0 102-103 2.675 0.0 0.0 0.0 0.0 104-105 2.8 0.0 0.0 0.0 0.0 106-107 3.25 0.0 0.0 0.0 0.0 108-109 3.6125 0.0 0.0 0.0 0.0 110-111 3.975 0.0 0.0 0.0 0.0 112-113 4.3875 0.0 0.0 0.0 0.0 114-115 4.762499999999999 0.0 0.0 0.0 0.0 116-117 5.387499999999999 0.0 0.0 0.0 0.0 118-119 5.7625 0.0 0.0 0.0 0.0 120-121 6.175000000000001 0.0 0.0 0.0 0.0 122-123 6.95 0.0 0.0 0.0 0.0 124-125 7.775 0.0 0.0 0.0 0.0 126-127 8.524999999999999 0.0 0.0 0.0 0.0 128-129 9.3125 0.0 0.0 0.0 0.0 130-131 9.75 0.0 0.0 0.0 0.0 132-133 10.4375 0.0 0.0 0.0 0.0 134-135 11.100000000000001 0.0 0.0 0.0 0.0 136-137 11.8125 0.0 0.0 0.0 0.0 138-139 12.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAAAAG 10 0.0068062083 145.15189 1 GGCCCGA 10 0.007070461 143.3375 5 GTGTGGC 30 0.0017881831 72.57595 145 GGGGGGG 140 1.01642785E-4 10.367992 140-144 >>END_MODULE Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974868 spots for SRR13165346.sra Written 1974868 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra Read 1974861 spots for SRR13165346.sra Written 1974861 spots for SRR13165346.sra SRR ids: ['SRR13165346.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_isica_r_ SRR13165346.sra spots: 39497227 blocks: [[1, 1974861], [1974862, 3949722], [3949723, 5924583], [5924584, 7899444], [7899445, 9874305], [9874306, 11849166], [11849167, 13824027], [13824028, 15798888], [15798889, 17773749], [17773750, 19748610], [19748611, 21723471], [21723472, 23698332], [23698333, 25673193], [25673194, 27648054], [27648055, 29622915], [29622916, 31597776], [31597777, 33572637], [33572638, 35547498], [35547499, 37522359], [37522360, 39497227]] SRR13165346 file size 13401185 SRR13165346 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165346 SRR13165346_1.fastq SRR13165346_2.fastq Input file: SRR13165346_1.fastq Paired file: SRR13165346_2.fastq trimmed: SRR13165346-trimmed-pair1.fastq, SRR13165346-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 15:42:56 2024 >> started Sat Dec 7 15:43:38 2024 >> done (42.035s) 39497227 read pairs processed; of these: 681 ( 0.00%) short read pairs filtered out after trimming by size control 15631 ( 0.04%) empty read pairs filtered out after trimming by size control 39480915 (99.96%) read pairs available; of these: 6999447 (17.73%) trimmed read pairs available after processing 32481468 (82.27%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 32 0.00% 19 37 0.00% 20 37 0.00% 21 52 0.00% 22 63 0.00% 23 46 0.00% 24 61 0.00% 25 75 0.00% 26 85 0.00% 27 80 0.00% 28 95 0.00% 29 103 0.00% 30 92 0.00% 31 135 0.00% 32 190 0.00% 33 152 0.00% 34 150 0.00% 35 175 0.00% 36 184 0.00% 37 201 0.00% 38 206 0.00% 39 216 0.00% 40 244 0.00% 41 292 0.00% 42 390 0.00% 43 322 0.00% 44 344 0.00% 45 408 0.00% 46 386 0.00% 47 452 0.00% 48 486 0.00% 49 547 0.00% 50 671 0.00% 51 682 0.00% 52 900 0.00% 53 925 0.00% 54 917 0.00% 55 1207 0.00% 56 1102 0.00% 57 1380 0.00% 58 1399 0.00% 59 1711 0.00% 60 1780 0.00% 61 2295 0.01% 62 2459 0.01% 63 2768 0.01% 64 3012 0.01% 65 3298 0.01% 66 3715 0.01% 67 4135 0.01% 68 4588 0.01% 69 5058 0.01% 70 5671 0.01% 71 6157 0.02% 72 7316 0.02% 73 8140 0.02% 74 9123 0.02% 75 10101 0.03% 76 11286 0.03% 77 12129 0.03% 78 13605 0.03% 79 14851 0.04% 80 16190 0.04% 81 18310 0.05% 82 20023 0.05% 83 22238 0.06% 84 24820 0.06% 85 26695 0.07% 86 28647 0.07% 87 30740 0.08% 88 33027 0.08% 89 34958 0.09% 90 37530 0.10% 91 40473 0.10% 92 42086 0.11% 93 45714 0.12% 94 48678 0.12% 95 51919 0.13% 96 55088 0.14% 97 58561 0.15% 98 60865 0.15% 99 63669 0.16% 100 66953 0.17% 101 68203 0.17% 102 70779 0.18% 103 73861 0.19% 104 76348 0.19% 105 78660 0.20% 106 82241 0.21% 107 85296 0.22% 108 88615 0.22% 109 91504 0.23% 110 92392 0.23% 111 94245 0.24% 112 97326 0.25% 113 99262 0.25% 114 101288 0.26% 115 105919 0.27% 116 106862 0.27% 117 109578 0.28% 118 111534 0.28% 119 113030 0.29% 120 115900 0.29% 121 117370 0.30% 122 118364 0.30% 123 119899 0.30% 124 121694 0.31% 125 123848 0.31% 126 126421 0.32% 127 127542 0.32% 128 129521 0.33% 129 131644 0.33% 130 132356 0.34% 131 134618 0.34% 132 137333 0.35% 133 138086 0.35% 134 137647 0.35% 135 139257 0.35% 136 140228 0.36% 137 139694 0.35% 138 140186 0.36% 139 144676 0.37% 140 145336 0.37% 141 146093 0.37% 142 149356 0.38% 143 148709 0.38% 144 150370 0.38% 145 151905 0.38% 146 150431 0.38% 147 153379 0.39% 148 155054 0.39% 149 154934 0.39% 150 154750 0.39% 151 32481468 82.27% 39480915 reads passed initial QC criterion=sequence-density sequence-density=0.64 sequence-density-rank=1 fanout-score=2.33 fanout-score-rank=22 prefix-density=0.68 prefix-fanout=2.2 sequence=GTGGCGTCGGTGCACCCGAACATGGGCAGCTTCCACATTGTCCAGTACCTGCCATCATAGTACCCAGGGGAGCTGTTGTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=89.12 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=7.1 sequence=CATCAATGATAATCCCAAAGTAGGAAGTGTGTACGTAGTAGCAAGTGTTATACATACATAAAATTAAGCGATGCGTTTCGATCAAGTACTTGGCATCATCGATCACATACGTACATTTCACAGCAGGTAGCTACGTACATTACAGGCATACGTACATGCAGAGAGATACCCGGCCCTGTGTTGTGTTTGCAATTGCATAGATGAGAATGAGATGACTTGATTCTTAATTAGCCGGCGAGAGCGCTGGTGTTGTAGACGAGGAGGGCGCCGCCGCCGAGGAGGCCGGCCAGGGTGACGGCCCAGATCAGGAGCCCCGTCGTCCCACCGGCGTAGCGGTCGCCAGATTCGGACCATACCTCCGGCGTGTAGATCGGGCTGTAGCCGTCGACGTTGGCGCCGTACTTGTCAACAAACTGGTACACACCCTTTCCCGTGCCCTTCCTGCCGTTGGCGTCGATGTCCACCGTCAAGCCACCTCCGATCCCGAGGGGCTTGTCGACCTTGATCTTCT criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=3.55 fanout-score-rank=14 prefix-density=0.60 prefix-fanout=3.2 sequence=GAGTTCAGCAAGGTCGGCTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=29 fanout-score=94.75 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=10.8 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGATGTCTGCTACCTTCTCGTCCACCGTCGGAGCTCCGGCTTCTACGCCAACCAGCTTCCTTGGGAAGAAGCTCAAGAAGCAGGTGACCTCGGCCGTGAACTACCATGGCAAGAGCACCAAGGCCAACAGATTCACAGTCATGGCCAAGGAGGTGGACGAGTCAAAGCAGACTGACCAGGACAGGTGGAAGGGCCTCGCCTACGATATCTCCGACGACCAGCAGGACATCACCAGGGGGAAGGGTATCGTCGACTCGCTCTTCCAGGCGCCCATGGGCGACGGTACCCACGTGGCCGTCCTCAGCTCCCAAGAGTACATCAGCCAGGGCCTAAGGAAGTACGACTTCGACAACA SRR13165346 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 15:46:37 Started mapping on | Dec 07 15:46:37 Finished on | Dec 07 15:49:19 Mapping speed, Million of reads per hour | 877.35 Number of input reads | 39480915 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 32485313 Uniquely mapped reads % | 82.28% Average mapped length | 287.63 Number of splices: Total | 33355172 Number of splices: Annotated (sjdb) | 31278360 Number of splices: GT/AG | 32917012 Number of splices: GC/AG | 373453 Number of splices: AT/AC | 14484 Number of splices: Non-canonical | 50223 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.90 Insertion rate per base | 0.01% Insertion average length | 2.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 318589 % of reads mapped to multiple loci | 0.81% Number of reads mapped to too many loci | 86549 % of reads mapped to too many loci | 0.22% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 15.95% % of reads unmapped: other | 0.74% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6677479 6677479 6677479 N_multimapping 318589 318589 318589 N_noFeature 1041568 31669842 1285335 N_ambiguous 733137 5338 161774 UnstrandedReadsAssigned:30710608 PositiveStrandReadsAssigned:810133 NegativeStrandReadsAssigned:31038204 Dataset is classified negative stranded MeadianReadLen=143 20thPercentileLength=143 echo kmer=139 SRR13165346 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165346-trimmed-pair1.fastq SRR13165346-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 39,480,915 reads, 36,606,287 reads pseudoaligned [quant] estimated average fragment length: 235.817 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,382 rounds 52973 SRR13165346.ke.tsv 35125 SRR13165346.se.tsv 88098 total ==> SRR13165346.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 701.943 0 0 PNS24247 1044 809.183 65.9431 3.28391 PNS24249 1928 1693.18 242.547 5.77246 PNS24246 1044 809.183 65.9431 3.28391 PNS24248 1044 809.183 65.9431 3.28391 PNS24244 1471 1236.18 80.6238 2.62815 PNS24243 293 113.906 0 0 KQK14069 1603 1368.18 409.564 12.0628 KQK14071 474 258.953 2.38123 0.370553 ==> SRR13165346.se.tsv <== BRADI_1g14170v3 362 BRADI_1g53295v3 174 BRADI_1g59795v3 517 BRADI_1g07683v3 0 BRADI_1g00485v3 8 BRADI_1g20270v3 677 BRADI_1g74790v3 400 BRADI_1g09890v3 2 BRADI_1g77505v3 460 BRADI_1g48960v3 0 SRR13165346 completed mapping pipeline successfully