Starting /dee2/code/volunteer_pipeline.sh SRR13165351 current disk space = 1542269415424 free memory = 1597475736 SRR13165351 SRAfilesize 36ec8af73e0c14e7911a7e4f247eb906 SRR13165351.sra SRR13165351.sra file validated SRR13165351 is paired end SRR13165351 is conventional basespace SRR13165351 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165351_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.588 37.0 37.0 37.0 37.0 37.0 2 36.1545 37.0 37.0 37.0 37.0 37.0 3 36.5425 37.0 37.0 37.0 37.0 37.0 4 36.5525 37.0 37.0 37.0 37.0 37.0 5 36.517 37.0 37.0 37.0 37.0 37.0 6 36.596 37.0 37.0 37.0 37.0 37.0 7 36.482 37.0 37.0 37.0 37.0 37.0 8 36.549 37.0 37.0 37.0 37.0 37.0 9 36.5305 37.0 37.0 37.0 37.0 37.0 10-14 36.5441 37.0 37.0 37.0 37.0 37.0 15-19 36.5055 37.0 37.0 37.0 37.0 37.0 20-24 36.5256 37.0 37.0 37.0 37.0 37.0 25-29 36.4723 37.0 37.0 37.0 37.0 37.0 30-34 36.42739999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.3735 37.0 37.0 37.0 37.0 37.0 40-44 36.42 37.0 37.0 37.0 37.0 37.0 45-49 36.3265 37.0 37.0 37.0 37.0 37.0 50-54 36.3147 37.0 37.0 37.0 37.0 37.0 55-59 36.339800000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.2869 37.0 37.0 37.0 37.0 37.0 65-69 36.23479999999999 37.0 37.0 37.0 37.0 37.0 70-74 36.243500000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.230599999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.0977 37.0 37.0 37.0 37.0 37.0 85-89 36.1845 37.0 37.0 37.0 37.0 37.0 90-94 36.0918 37.0 37.0 37.0 37.0 37.0 95-99 36.0796 37.0 37.0 37.0 37.0 37.0 100-104 36.0603 37.0 37.0 37.0 37.0 37.0 105-109 36.1278 37.0 37.0 37.0 37.0 37.0 110-114 36.0443 37.0 37.0 37.0 37.0 37.0 115-119 36.0837 37.0 37.0 37.0 37.0 37.0 120-124 35.9368 37.0 37.0 37.0 37.0 37.0 125-129 35.926 37.0 37.0 37.0 37.0 37.0 130-134 35.9273 37.0 37.0 37.0 37.0 37.0 135-139 35.8677 37.0 37.0 37.0 37.0 37.0 140-144 35.7447 37.0 37.0 37.0 37.0 37.0 145-149 35.4967 37.0 37.0 37.0 37.0 37.0 150-151 35.30475 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 3.0 24 5.0 25 4.0 26 9.0 27 8.0 28 14.0 29 24.0 30 23.0 31 41.0 32 55.0 33 80.0 34 143.0 35 334.0 36 2840.0 37 416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.05 10.95 6.6000000000000005 32.4 2 24.031202818319073 10.241570206341217 31.202818319073984 34.52440865626573 3 20.549999999999997 14.7 26.125 38.625 4 26.150000000000002 18.85 23.35 31.65 5 28.625 25.25 23.575 22.55 6 25.575 28.999999999999996 21.349999999999998 24.075 7 18.075 25.25 37.375 19.3 8 19.1 23.95 29.575000000000003 27.375 9 20.7 21.275 33.15 24.875 10-14 23.155 26.025 25.865 24.955 15-19 23.7 24.560000000000002 25.88 25.86 20-24 23.205000000000002 25.3 25.5 25.995 25-29 23.195 25.255 25.715 25.835 30-34 23.425 24.19 26.314999999999998 26.07 35-39 23.125 24.5 25.655 26.72 40-44 23.11 25.165 25.06 26.665 45-49 23.855 24.195 25.795 26.155 50-54 24.265 23.794999999999998 25.535000000000004 26.405 55-59 23.799999999999997 24.87 25.405 25.924999999999997 60-64 23.73 24.815 25.724999999999998 25.729999999999997 65-69 23.150000000000002 25.21 25.41 26.229999999999997 70-74 24.11 24.55 25.119999999999997 26.22 75-79 24.104999999999997 24.09 25.245 26.56 80-84 24.505 24.05 25.155 26.290000000000003 85-89 24.095 24.995 24.779999999999998 26.13 90-94 23.835 25.074999999999996 25.009999999999998 26.08 95-99 24.675 24.695 24.825 25.805 100-104 25.215 24.6 24.834999999999997 25.35 105-109 25.555 24.625 23.799999999999997 26.02 110-114 24.92 24.955 24.965 25.16 115-119 24.104999999999997 25.025 24.435000000000002 26.435 120-124 25.145 24.875 24.04 25.94 125-129 24.69 24.6 24.404999999999998 26.305 130-134 24.615000000000002 24.89 24.84 25.655 135-139 25.540000000000003 25.025 24.0 25.435000000000002 140-144 25.235000000000003 25.295 23.84 25.629999999999995 145-149 25.11 24.545 24.33 26.015 150-151 24.675 24.175 24.8 26.35 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 1.0 3 2.0 4 1.5 5 0.5 6 1.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 1.5 25 2.5 26 1.5 27 2.0 28 3.0 29 3.0 30 6.0 31 16.0 32 20.5 33 20.5 34 29.0 35 40.0 36 50.0 37 62.5 38 74.0 39 82.0 40 98.0 41 120.5 42 160.0 43 171.5 44 176.0 45 204.0 46 181.5 47 166.0 48 189.5 49 174.0 50 147.0 51 141.5 52 138.5 53 128.0 54 108.5 55 87.5 56 90.0 57 110.0 58 99.0 59 88.5 60 88.5 61 81.5 62 70.5 63 63.5 64 56.5 65 53.0 66 56.5 67 49.5 68 40.5 69 32.0 70 27.5 71 29.5 72 29.0 73 23.5 74 20.5 75 17.5 76 15.5 77 12.0 78 9.0 79 6.0 80 4.5 81 5.0 82 2.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.65 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.675 #Duplication Level Percentage of deduplicated Percentage of total 1 72.82874084408789 52.2 2 19.114056505057555 27.400000000000002 3 5.3714684339030345 11.55 4 1.5695849319846529 4.5 5 0.7673526334147193 2.75 6 0.24415765608650158 1.05 7 0.06975933031042902 0.35000000000000003 8 0.03487966515521451 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGTCGATCTCGTAT 8 0.2 TruSeq Adapter, Index 3 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit GCCATAGTTGTCGTCATGGAACTCTCCTGGATCTTCATTCTCAACCACTG 7 0.17500000000000002 No Hit GTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC 6 0.15 No Hit GCTGAGTCTTGGCTAGCACCTTTCATACCAACAACATCAACTTCACTTTG 6 0.15 No Hit GGAATACATAATTTTTTATCATGCCAACAAATGGTATCCTTGTATAATGT 6 0.15 No Hit GCTGACTATAATAAATCCAGTCCTCATGCTCGCCATCAATCTTTGCGTGT 6 0.15 No Hit CCCCCTTCTGCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGAA 6 0.15 No Hit GCCCGAGCACCAGTTGCAATTATAAGTGTCTTATAGCTGATAGTTTCTCC 6 0.15 No Hit GCCTCCTTCCGATCCGTCAATACATTGTCCATTCCTTTCATCTCAATTGG 6 0.15 No Hit CTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAAT 5 0.125 No Hit CACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTCCAGCTTGGTA 5 0.125 No Hit GGGCAGCATTAACAATTTTTTCAATGTCAGAGAGTGTGCCAAATGCGAAC 5 0.125 No Hit GCCGTACATGGTGAGGCAGAGGCTGAGGATGGTGACGAGGCCCGCGGCGC 5 0.125 No Hit GTGGTGCCAAAAATCTGAAATATTCCAAGGAATGAAAAATACCCTCAGAA 5 0.125 No Hit GCTGCGGCTTCTCAAAGCTAAACGGGAGAATAGCTTTCGCCTCCAACTCT 5 0.125 No Hit CCTCGTACAGTTTACTTACTGTCTACAGATCCATTTCTGTCCATACATAG 5 0.125 No Hit CTACCGACTATGATAACAAATAGCATCACACAGCAATTCAGGACTGTCAC 5 0.125 No Hit GGGTTACACATACTACAACGTGCTCAACCGAATCCTGCAGCATATATGTG 5 0.125 No Hit GCAGGTAGAGGGGATTTTTCATGCTTTAAATCGGACTTCGTGGATTTGTG 5 0.125 No Hit GTGAGCTGCCCGGGAGTGGTGATAAACAGGTAGCTGGTCTCCCTGCCCAC 5 0.125 No Hit GCCTCAATGTCAGGTGAAGAAGACGGGACAACATGGAGTGGAGCTTTAAA 5 0.125 No Hit GGGTGTAGCAAAAGTGGGACGATACAAGACATTTCTTCGTTCAAGTTTCA 5 0.125 No Hit TACTCATGAATACAGTTAATCCGGGTTGTTGGGAGAGATCACATGCATAA 5 0.125 No Hit GTCCAATCTGTGATCTTTGCAGCAATCGCTTTTGTCTGTTCAGCAACAAC 5 0.125 No Hit GTCCGCATGTGAAGCAAGCACCACTGAAGCTTCTGTCCCTACTGCCAAAG 5 0.125 No Hit AGCTGGACCTCCTTTTGTCTTGTCCTACCACTCCTATCATTTCCAGTACC 5 0.125 No Hit GGCCGATCGCCCTTTCCAACTGTTAATTTCCCTATGCACAAATTAACCAG 5 0.125 No Hit CTCTGAACAGCTAATTTAGTATTCCCTCGGTCCGTCCAGAAATAAGTGAC 5 0.125 No Hit CCTGGTGTCAAATAAGAGCCCCCTTCATATGGATCTGTAAACTCAATAGC 5 0.125 No Hit GTAGGGACAGTCCACGTATGCATCAATCAATTTGAATCCGAACACAAAAA 5 0.125 No Hit GGTTAGTATCACCAGCTGAAAGGTTATTGCAAGATGCTTGCTGCTCCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1625 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.6499999999999999 0.0 0.0 0.0 0.0 86-87 0.85 0.0 0.0 0.0 0.0 88-89 1.0125 0.0 0.0 0.0 0.0 90-91 1.25 0.0 0.0 0.0 0.0 92-93 1.5 0.0 0.0 0.0 0.0 94-95 1.875 0.0 0.0 0.0 0.0 96-97 2.275 0.0 0.0 0.0 0.0 98-99 2.7 0.0 0.0 0.0 0.0 100-101 3.3625 0.0 0.0 0.0 0.0 102-103 3.8 0.0 0.0 0.0 0.0 104-105 4.1875 0.0 0.0 0.0 0.0 106-107 4.5375 0.0 0.0 0.0 0.0 108-109 5.0 0.0 0.0 0.0 0.0 110-111 5.45 0.0 0.0 0.0 0.0 112-113 5.9875 0.0 0.0 0.0 0.0 114-115 6.725 0.0 0.0 0.0 0.0 116-117 7.625 0.0 0.0 0.0 0.0 118-119 8.4 0.0 0.0 0.0 0.0 120-121 9.025 0.0 0.0 0.0 0.0 122-123 9.662500000000001 0.0 0.0 0.0 0.0 124-125 10.35 0.0 0.0 0.0 0.0 126-127 11.0375 0.0 0.0 0.0 0.0 128-129 11.7625 0.0 0.0 0.0 0.0 130-131 12.6 0.0 0.0 0.0 0.0 132-133 13.5625 0.0 0.0 0.0 0.0 134-135 14.2875 0.0 0.0 0.0 0.0 136-137 14.9125 0.0 0.0 0.0 0.0 138-139 15.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCTGT 10 0.006830828 145.0 1 >>END_MODULE SRR13165351 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165351_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.12625 37.0 37.0 37.0 37.0 37.0 2 36.161 37.0 37.0 37.0 37.0 37.0 3 36.275 37.0 37.0 37.0 37.0 37.0 4 36.29 37.0 37.0 37.0 37.0 37.0 5 36.3765 37.0 37.0 37.0 37.0 37.0 6 36.3635 37.0 37.0 37.0 37.0 37.0 7 36.2785 37.0 37.0 37.0 37.0 37.0 8 36.341 37.0 37.0 37.0 37.0 37.0 9 36.296 37.0 37.0 37.0 37.0 37.0 10-14 36.2529 37.0 37.0 37.0 37.0 37.0 15-19 36.2048 37.0 37.0 37.0 37.0 37.0 20-24 36.221199999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.16010000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.0659 37.0 37.0 37.0 37.0 37.0 35-39 36.0498 37.0 37.0 37.0 37.0 37.0 40-44 36.0968 37.0 37.0 37.0 37.0 37.0 45-49 36.033899999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.03079999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.0034 37.0 37.0 37.0 37.0 37.0 60-64 36.0446 37.0 37.0 37.0 37.0 37.0 65-69 35.9766 37.0 37.0 37.0 37.0 37.0 70-74 35.9482 37.0 37.0 37.0 37.0 37.0 75-79 35.9585 37.0 37.0 37.0 37.0 37.0 80-84 35.9649 37.0 37.0 37.0 37.0 37.0 85-89 35.8343 37.0 37.0 37.0 37.0 37.0 90-94 35.8386 37.0 37.0 37.0 37.0 37.0 95-99 35.8313 37.0 37.0 37.0 37.0 37.0 100-104 35.797399999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.804500000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.6619 37.0 37.0 37.0 37.0 37.0 115-119 35.6873 37.0 37.0 37.0 37.0 37.0 120-124 35.5472 37.0 37.0 37.0 37.0 37.0 125-129 35.5589 37.0 37.0 37.0 37.0 37.0 130-134 35.277300000000004 37.0 37.0 37.0 32.2 37.0 135-139 35.3146 37.0 37.0 37.0 37.0 37.0 140-144 35.028999999999996 37.0 37.0 37.0 29.8 37.0 145-149 34.917 37.0 37.0 37.0 25.0 37.0 150-151 34.47325 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 5.0 15 2.0 16 4.0 17 3.0 18 1.0 19 3.0 20 5.0 21 2.0 22 5.0 23 7.0 24 6.0 25 10.0 26 7.0 27 11.0 28 10.0 29 9.0 30 25.0 31 29.0 32 53.0 33 134.0 34 195.0 35 542.0 36 2705.0 37 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.846925972396484 20.95357590966123 9.159347553324968 24.040150564617317 2 33.074999999999996 21.65 24.975 20.3 3 25.3 25.374999999999996 28.1 21.224999999999998 4 27.800000000000004 31.775 18.775 21.65 5 29.799999999999997 31.225 18.675 20.3 6 25.474999999999998 34.8 19.0 20.724999999999998 7 26.25 20.175 31.025000000000002 22.55 8 25.374999999999996 22.575 24.224999999999998 27.825 9 24.65 21.85 26.875 26.625 10-14 26.71 26.11 22.405 24.775 15-19 26.265 25.419999999999998 23.53 24.785 20-24 26.525 24.765 24.135 24.575 25-29 27.075 24.765 23.765 24.395 30-34 26.365 24.425 24.279999999999998 24.93 35-39 26.605 25.94 23.285 24.169999999999998 40-44 26.705000000000002 25.22 24.005000000000003 24.07 45-49 26.33 25.345000000000002 23.64 24.685000000000002 50-54 26.72 25.345000000000002 23.195 24.740000000000002 55-59 27.525 24.34 23.945 24.19 60-64 26.640000000000004 25.064999999999998 23.54 24.755 65-69 26.63 25.46 23.635 24.275 70-74 27.46 24.03 23.775 24.735 75-79 26.355 24.87 23.775 25.0 80-84 26.119999999999997 25.105 23.765 25.009999999999998 85-89 26.169999999999998 25.615 23.355 24.86 90-94 26.52 25.080000000000002 23.200000000000003 25.2 95-99 26.545 25.94 23.835 23.68 100-104 27.884999999999998 25.679999999999996 23.03 23.405 105-109 27.095000000000002 25.669999999999998 23.87 23.365 110-114 27.46 25.505 23.03 24.005000000000003 115-119 28.389999999999997 25.22 23.745 22.645 120-124 28.43 25.7 23.205000000000002 22.665 125-129 28.34 25.825 22.88 22.955000000000002 130-134 28.725 25.19 23.990000000000002 22.095000000000002 135-139 29.220000000000002 24.959999999999997 23.41 22.41 140-144 29.604999999999997 25.645 23.085 21.665 145-149 30.28 25.06 23.625 21.035 150-151 31.85 24.8625 22.825 20.4625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 1.0 14 1.5 15 1.0 16 1.0 17 1.0 18 0.5 19 0.0 20 1.0 21 3.0 22 2.5 23 0.5 24 1.5 25 2.5 26 2.0 27 2.5 28 3.0 29 6.0 30 11.0 31 13.0 32 13.5 33 10.0 34 12.0 35 27.5 36 48.0 37 57.5 38 61.5 39 83.0 40 104.0 41 126.0 42 152.0 43 167.5 44 173.5 45 166.0 46 165.0 47 174.0 48 161.5 49 149.5 50 144.5 51 134.5 52 132.0 53 122.0 54 122.0 55 111.0 56 100.0 57 110.5 58 107.0 59 93.0 60 76.0 61 73.0 62 78.5 63 75.5 64 63.0 65 54.5 66 56.5 67 55.0 68 56.5 69 56.0 70 48.0 71 37.0 72 32.5 73 29.5 74 23.5 75 19.0 76 19.0 77 17.5 78 9.0 79 5.5 80 3.0 81 2.0 82 2.0 83 2.5 84 1.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 1.0 94 1.0 95 0.5 96 1.0 97 1.0 98 2.5 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.3 #Duplication Level Percentage of deduplicated Percentage of total 1 73.96265560165975 53.474999999999994 2 18.499308437067775 26.75 3 4.8063623789764875 10.424999999999999 4 1.417704011065007 4.1000000000000005 5 0.7607192254495159 2.75 6 0.4495159059474412 1.95 7 0.06915629322268327 0.35000000000000003 8 0.034578146611341634 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 8 0.2 No Hit AGGCAAGTGAAACTGTTGATGTGGAAGATGCCAAGCACATTCTGCAGAAT 7 0.17500000000000002 No Hit ATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTTACTGTT 7 0.17500000000000002 No Hit GAAAAGATGAGGTTTCAGTTATTAAATCAGGGCTGAGAAAGGTGAAAATC 6 0.15 No Hit GAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAA 6 0.15 No Hit CGAAACAAAAGCTGTACAGATTGATGACATCTCTAAAGGTTCTTCTAGGG 6 0.15 No Hit GGAAACTGCAACTGCGGGTCATCCTGCAAGTGCGGCAGCGGCTGCAACGG 6 0.15 No Hit CACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAA 6 0.15 No Hit GATACAATCTGCTGCCAGTGCTCTCATGGCCGAGCACACAAAGTTGCGCA 6 0.15 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 6 0.15 No Hit GGATTTCATACTTGTGTTGGTGCTAATGATGAGTTACTGACGACAAAATG 6 0.15 No Hit GCTTAAATGAGAAGTGTATGCCATCACAACGATGACTTTTTTCTTCTTCC 6 0.15 No Hit TGTTACTATTGTACCATTTGGTTGCGTCGGAGAAGATGACTTTCTTCAGA 6 0.15 No Hit GGGAGGGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTTTGCTCGCTG 6 0.15 No Hit GTTCGGCATCGCGTTTAACGGCGGGTTTAACCAGCCGATCATGTGCGGTG 6 0.15 No Hit GGCAGGAGCTTCAAACCTGACAATGATATTTGGTCAGATGATGACTTTTC 6 0.15 No Hit GAAGCTCACGCTGCAGCTGGATTTGGAGAAGCCCAGCCTCGGTGACGAAA 5 0.125 No Hit GGTGCTTGCTATGTCCGGAAATAAGTGAATCTTGTACAAAACGAAGTCAC 5 0.125 No Hit GTTCTGATTGCTGTGCAGATTACTGTGTAACTATGGAGTTATGGATTGCT 5 0.125 No Hit TGTGTGATACCGTGAACAGTGAACACGATGGCGTGCTTCTAGTAAAAAAA 5 0.125 No Hit GGAGACGATGACTGCCTCCTCCCCCTTGAACAATGGGCAGAGATCGCCAA 5 0.125 No Hit CAAGGAGGGCATGATGGAGAAGATCAAGGACAAGATCACCGGCGAGCACG 5 0.125 No Hit GATTAAGCATCGCCGTAGAGGATGTCGGTACCTTTTTGGTTTCTCTATTT 5 0.125 No Hit GCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTACGGGTC 5 0.125 No Hit GTCGATATCTGTAGATTTTAGGTTTTTGCTTCGTTTTTGTGGCGTTTTGA 5 0.125 No Hit CAAAGATCATCAGCCCCAAGGAATATCAGAGTTTCAGGATCGGAAGTGAT 5 0.125 No Hit GTGACCAAGAGGAAAATCTTGATTTGACATCTCTTTCATCCTTTAAAAGA 5 0.125 No Hit GCGGCGGAGAACGACGGCAAGTGCAAGTGCGGCACCAGCTGCACCTGCAC 5 0.125 No Hit ACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTCGAG 5 0.125 No Hit GGAAGCGCGTACCATTATTCATACATCTGCGTTGGTGAAGGAGTTGCATG 5 0.125 No Hit GGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAAGGCC 5 0.125 No Hit TGGAGACCCCGGTGACGTCGAGCCCGCTGGTGGCGTGGTACCTCTCGAAC 5 0.125 No Hit AGCGTCTTCACAGTCTGATGAAAGTGGAAACGGAGAGGTTTCTCTCCACA 5 0.125 No Hit AAGAAGGAGTACCCTGACGCCTATGTCCGCATCATCGGCTTCGACAACAC 5 0.125 No Hit GACATTCTCTTTCTGCCATAAGTTGTTCAGAGGGATCATAATTTCAATCA 5 0.125 No Hit AGAACCTCTTTCTGAGGCTGAGCCTGCTACTGCTGAGGCACTTGAACTCG 5 0.125 No Hit CTTGAAGGTCATTGCATGTGTTGGCGAGACTCTCGAGCAGCGGGAAGCTG 5 0.125 No Hit GTCTGGTATTGTACATATTGCAAGCACTTATAAATTGCAATGTCAAGCAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1625 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.6499999999999999 0.0 0.0 0.0 0.0 86-87 0.85 0.0 0.0 0.0 0.0 88-89 1.0125 0.0 0.0 0.0 0.0 90-91 1.25 0.0 0.0 0.0 0.0 92-93 1.5 0.0 0.0 0.0 0.0 94-95 1.875 0.0 0.0 0.0 0.0 96-97 2.25 0.0 0.0 0.0 0.0 98-99 2.675 0.0 0.0 0.0 0.0 100-101 3.3375 0.0 0.0 0.0 0.0 102-103 3.7625 0.0 0.0 0.0 0.0 104-105 4.1625 0.0 0.0 0.0 0.0 106-107 4.5125 0.0 0.0 0.0 0.0 108-109 4.9625 0.0 0.0 0.0 0.0 110-111 5.425 0.0 0.0 0.0 0.0 112-113 5.9625 0.0 0.0 0.0 0.0 114-115 6.7125 0.0 0.0 0.0 0.0 116-117 7.625 0.0 0.0 0.0 0.0 118-119 8.3875 0.0 0.0 0.0 0.0 120-121 9.025 0.0 0.0 0.0 0.0 122-123 9.662500000000001 0.0 0.0 0.0 0.0 124-125 10.35 0.0 0.0 0.0 0.0 126-127 11.0375 0.0 0.0 0.0 0.0 128-129 11.774999999999999 0.0 0.0 0.0 0.0 130-131 12.625 0.0 0.0 0.0 0.0 132-133 13.587499999999999 0.0 0.0 0.0 0.0 134-135 14.2875 0.0 0.0 0.0 0.0 136-137 14.9125 0.0 0.0 0.0 0.0 138-139 15.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCTCT 10 0.006830828 145.0 9 >>END_MODULE Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401717 spots for SRR13165351.sra Written 1401717 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra Read 1401716 spots for SRR13165351.sra Written 1401716 spots for SRR13165351.sra SRR ids: ['SRR13165351.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ygxqmesb SRR13165351.sra spots: 28034321 blocks: [[1, 1401716], [1401717, 2803432], [2803433, 4205148], [4205149, 5606864], [5606865, 7008580], [7008581, 8410296], [8410297, 9812012], [9812013, 11213728], [11213729, 12615444], [12615445, 14017160], [14017161, 15418876], [15418877, 16820592], [16820593, 18222308], [18222309, 19624024], [19624025, 21025740], [21025741, 22427456], [22427457, 23829172], [23829173, 25230888], [25230889, 26632604], [26632605, 28034321]] SRR13165351 file size 9505588 SRR13165351 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165351 SRR13165351_1.fastq SRR13165351_2.fastq Input file: SRR13165351_1.fastq Paired file: SRR13165351_2.fastq trimmed: SRR13165351-trimmed-pair1.fastq, SRR13165351-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 15:45:56 2024 >> started Sat Dec 7 15:46:42 2024 >> done (45.724s) 28034321 read pairs processed; of these: 644 ( 0.00%) short read pairs filtered out after trimming by size control 41117 ( 0.15%) empty read pairs filtered out after trimming by size control 27992560 (99.85%) read pairs available; of these: 5750872 (20.54%) trimmed read pairs available after processing 22241688 (79.46%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 36 0.00% 19 35 0.00% 20 42 0.00% 21 34 0.00% 22 39 0.00% 23 52 0.00% 24 62 0.00% 25 66 0.00% 26 82 0.00% 27 72 0.00% 28 77 0.00% 29 101 0.00% 30 115 0.00% 31 135 0.00% 32 124 0.00% 33 140 0.00% 34 131 0.00% 35 160 0.00% 36 157 0.00% 37 190 0.00% 38 178 0.00% 39 207 0.00% 40 223 0.00% 41 263 0.00% 42 282 0.00% 43 314 0.00% 44 324 0.00% 45 276 0.00% 46 364 0.00% 47 402 0.00% 48 378 0.00% 49 501 0.00% 50 584 0.00% 51 632 0.00% 52 740 0.00% 53 817 0.00% 54 828 0.00% 55 998 0.00% 56 1070 0.00% 57 1160 0.00% 58 1370 0.00% 59 1576 0.01% 60 1738 0.01% 61 1891 0.01% 62 2189 0.01% 63 2529 0.01% 64 3085 0.01% 65 3209 0.01% 66 3586 0.01% 67 3649 0.01% 68 4449 0.02% 69 4714 0.02% 70 5424 0.02% 71 6186 0.02% 72 7010 0.03% 73 8110 0.03% 74 8655 0.03% 75 9684 0.03% 76 10533 0.04% 77 11217 0.04% 78 12680 0.05% 79 14134 0.05% 80 15166 0.05% 81 17022 0.06% 82 18608 0.07% 83 20986 0.07% 84 23356 0.08% 85 25408 0.09% 86 27412 0.10% 87 28532 0.10% 88 31082 0.11% 89 32165 0.11% 90 34365 0.12% 91 36275 0.13% 92 38334 0.14% 93 41513 0.15% 94 44108 0.16% 95 47140 0.17% 96 50212 0.18% 97 52526 0.19% 98 54385 0.19% 99 56353 0.20% 100 58851 0.21% 101 60276 0.22% 102 61963 0.22% 103 63645 0.23% 104 65565 0.23% 105 67538 0.24% 106 70905 0.25% 107 72536 0.26% 108 74550 0.27% 109 76468 0.27% 110 77670 0.28% 111 78976 0.28% 112 81321 0.29% 113 81167 0.29% 114 83802 0.30% 115 86230 0.31% 116 89478 0.32% 117 90303 0.32% 118 91559 0.33% 119 92653 0.33% 120 95801 0.34% 121 95977 0.34% 122 95857 0.34% 123 97730 0.35% 124 99461 0.36% 125 101306 0.36% 126 102255 0.37% 127 103621 0.37% 128 103225 0.37% 129 105974 0.38% 130 106408 0.38% 131 106882 0.38% 132 107785 0.39% 133 108357 0.39% 134 107684 0.38% 135 109439 0.39% 136 110230 0.39% 137 109260 0.39% 138 111764 0.40% 139 114223 0.41% 140 114228 0.41% 141 114354 0.41% 142 116386 0.42% 143 115746 0.41% 144 115892 0.41% 145 117211 0.42% 146 116976 0.42% 147 120620 0.43% 148 119704 0.43% 149 123474 0.44% 150 122629 0.44% 151 22241688 79.46% 27992560 reads passed initial QC criterion=sequence-density sequence-density=0.71 sequence-density-rank=1 fanout-score=2.15 fanout-score-rank=26 prefix-density=0.72 prefix-fanout=2.1 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=29.05 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=6.8 sequence=TTGCATCAATGATAATCCCAAAGTAGGAAGTGTGTACGTAGTAGCAAGTGTTATACATACATAAAATTAAGCGATGCGTTTCGATCAAGTACTTGGCATCATCGATCACATACGTACATTTCACAGCAGGTAGCTACGTACATTACAGGCATACGTACATGCAGAGAGATACCCGGCCCTGTGTTGTGTTTGCAATTGCATAGATGAGAATGAGATGACTTGATTCTTAATTAGCCGGCGAGAGCGCTGGTGTTGTAGACGAGGAGGGCGCCGCCGCCGAGGAGGCCGGCCAGGGTGACGGCCCAGATCAGGAGCCCCGTCGTCCCACCGGCGTAGCGGTCGCCAGATTCGGACCATACCTCCGGCGTGTAGATCGGGCTGTAGCCGTCGACGTTGGCGCCGTACTTGTCAACAAACTGGTACACACCCTTTCCCGTGCCCTTCCTGCCGTTGGCGTCGATGTCCACCGTCAAGCCACCTCCGATCCCGAGGGGCTTGTCGACCTTGATCT criterion=sequence-density sequence-density=0.47 sequence-density-rank=1 fanout-score=2.50 fanout-score-rank=27 prefix-density=0.51 prefix-fanout=2.3 sequence=GGTGGTGCATGGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=34 fanout-score=67.76 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=3.9 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGATGTCTGCTACCTTCTCGTCCACCGTCGGAGCTCCGGCTTCTACGCCAACCAGCTTCCTTGGGAAGAAGCTCAAGAAGCAGGTGACCTCGGCCGTGAACTACCATGGCAAGAGCACCAAGGCCAACAGATTCACAGTCATGGCCAAGGAGGTGGACGAGTCAAAGCAGACTGACCAGGACAGGTGGAAGGGCCTCGCCTACGATATCTCCGACGACCAGCAGGACATCACCAGGGGGAAGGGTATCGTCGACTCGCTCTTCCAGGCGCCCATGGGCGACGGTACCCACGTGGCCGTCCTCAGCTCCCAAGAGTACATCAGCCAGGGCCTAAGGAAGTACGACTTCGACAACA SRR13165351 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 15:47:17 Started mapping on | Dec 07 15:47:17 Finished on | Dec 07 15:50:45 Mapping speed, Million of reads per hour | 484.49 Number of input reads | 27992560 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 25485555 Uniquely mapped reads % | 91.04% Average mapped length | 289.13 Number of splices: Total | 24505306 Number of splices: Annotated (sjdb) | 22859830 Number of splices: GT/AG | 24156600 Number of splices: GC/AG | 294145 Number of splices: AT/AC | 11157 Number of splices: Non-canonical | 43404 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.60 Insertion rate per base | 0.01% Insertion average length | 2.49 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 755365 % of reads mapped to multiple loci | 2.70% Number of reads mapped to too many loci | 163298 % of reads mapped to too many loci | 0.58% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.17% % of reads unmapped: other | 2.51% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1751955 1751955 1751955 N_multimapping 755365 755365 755365 N_noFeature 1271353 24738492 1500738 N_ambiguous 629658 4151 112351 UnstrandedReadsAssigned:23584544 PositiveStrandReadsAssigned:742912 NegativeStrandReadsAssigned:23872466 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=150 echo kmer=145 SRR13165351 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165351-trimmed-pair1.fastq SRR13165351-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,992,560 reads, 24,340,177 reads pseudoaligned [quant] estimated average fragment length: 235.568 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,220 rounds 52973 SRR13165351.ke.tsv 35125 SRR13165351.se.tsv 88098 total ==> SRR13165351.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 701.75 0 0 PNS24247 1044 809.432 87.214 6.12138 PNS24249 1928 1693.43 177.756 5.96348 PNS24246 1044 809.432 87.214 6.12138 PNS24248 1044 809.432 87.214 6.12138 PNS24244 1471 1236.43 129.602 5.95505 PNS24243 293 111.904 2 1.01538 KQK14069 1603 1368.43 1377.08 57.1716 KQK14071 474 257.201 33.5458 7.40985 ==> SRR13165351.se.tsv <== BRADI_1g14170v3 1511 BRADI_1g53295v3 125 BRADI_1g59795v3 858 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 578 BRADI_1g74790v3 626 BRADI_1g09890v3 0 BRADI_1g77505v3 400 BRADI_1g48960v3 0 SRR13165351 completed mapping pipeline successfully