Starting /dee2/code/volunteer_pipeline.sh SRR13165352 current disk space = 1542084288512 free memory = 1596233640 SRR13165352 SRAfilesize 1f1e51dfe33b8fabf79485ca7624cf9d SRR13165352.sra SRR13165352.sra file validated SRR13165352 is paired end SRR13165352 is conventional basespace SRR13165352 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165352_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5925 37.0 37.0 37.0 37.0 37.0 2 36.1325 37.0 37.0 37.0 37.0 37.0 3 36.548 37.0 37.0 37.0 37.0 37.0 4 36.587 37.0 37.0 37.0 37.0 37.0 5 36.5575 37.0 37.0 37.0 37.0 37.0 6 36.4835 37.0 37.0 37.0 37.0 37.0 7 36.5095 37.0 37.0 37.0 37.0 37.0 8 36.533 37.0 37.0 37.0 37.0 37.0 9 36.535 37.0 37.0 37.0 37.0 37.0 10-14 36.557500000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.5258 37.0 37.0 37.0 37.0 37.0 20-24 36.521 37.0 37.0 37.0 37.0 37.0 25-29 36.4245 37.0 37.0 37.0 37.0 37.0 30-34 36.4531 37.0 37.0 37.0 37.0 37.0 35-39 36.453 37.0 37.0 37.0 37.0 37.0 40-44 36.463 37.0 37.0 37.0 37.0 37.0 45-49 36.3857 37.0 37.0 37.0 37.0 37.0 50-54 36.4534 37.0 37.0 37.0 37.0 37.0 55-59 36.39 37.0 37.0 37.0 37.0 37.0 60-64 36.354 37.0 37.0 37.0 37.0 37.0 65-69 36.353899999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.298199999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.327 37.0 37.0 37.0 37.0 37.0 80-84 36.2986 37.0 37.0 37.0 37.0 37.0 85-89 36.2462 37.0 37.0 37.0 37.0 37.0 90-94 36.1957 37.0 37.0 37.0 37.0 37.0 95-99 36.21169999999999 37.0 37.0 37.0 37.0 37.0 100-104 36.2305 37.0 37.0 37.0 37.0 37.0 105-109 36.205 37.0 37.0 37.0 37.0 37.0 110-114 36.10339999999999 37.0 37.0 37.0 37.0 37.0 115-119 36.1098 37.0 37.0 37.0 37.0 37.0 120-124 36.048899999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.999199999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.96 37.0 37.0 37.0 37.0 37.0 135-139 35.8596 37.0 37.0 37.0 37.0 37.0 140-144 35.75 37.0 37.0 37.0 37.0 37.0 145-149 35.532300000000006 37.0 37.0 37.0 37.0 37.0 150-151 35.30025 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 3.0 25 1.0 26 3.0 27 8.0 28 10.0 29 14.0 30 22.0 31 47.0 32 50.0 33 76.0 34 144.0 35 323.0 36 2832.0 37 464.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.5 10.85 6.525 30.125 2 23.765120967741936 11.920362903225806 30.569556451612907 33.74495967741936 3 21.475 17.474999999999998 25.8 35.25 4 26.400000000000002 23.3 21.825 28.475 5 27.275 29.5 21.675 21.55 6 25.825 31.5 20.375 22.3 7 19.475 24.55 37.25 18.725 8 21.375 23.275000000000002 29.725 25.624999999999996 9 21.15 22.2 31.45 25.2 10-14 23.25 26.515 24.555 25.679999999999996 15-19 24.185000000000002 24.935 25.105 25.775 20-24 23.18 25.745 25.295 25.779999999999998 25-29 23.724999999999998 26.174999999999997 24.575 25.525 30-34 23.61 24.84 25.490000000000002 26.06 35-39 23.905 24.695 25.1 26.3 40-44 24.165 25.919999999999998 24.065 25.85 45-49 23.74 26.325 24.125 25.81 50-54 24.98 24.925 24.11 25.985000000000003 55-59 23.95 24.605 25.06 26.384999999999998 60-64 23.96 24.884999999999998 24.95 26.205000000000002 65-69 24.79 25.724999999999998 23.96 25.525 70-74 25.165 25.259999999999998 24.04 25.535000000000004 75-79 24.959999999999997 24.92 24.09 26.029999999999998 80-84 25.0 25.290000000000003 24.235 25.474999999999998 85-89 25.014999999999997 24.335 24.34 26.31 90-94 25.185000000000002 25.585 24.585 24.645 95-99 24.59 24.97 24.25 26.19 100-104 24.77 25.040000000000003 24.279999999999998 25.91 105-109 25.34 25.095 23.18 26.384999999999998 110-114 25.39 24.72 24.25 25.64 115-119 25.040000000000003 25.724999999999998 23.5 25.735000000000003 120-124 24.044999999999998 25.319999999999997 23.93 26.705000000000002 125-129 24.610000000000003 25.259999999999998 24.0 26.13 130-134 24.89 25.745 22.71 26.655 135-139 25.025 24.81 23.294999999999998 26.87 140-144 25.169999999999998 25.385 23.035 26.41 145-149 24.555 25.775 23.244999999999997 26.424999999999997 150-151 24.55 24.9 23.65 26.900000000000002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 0.5 26 0.0 27 3.0 28 3.0 29 3.0 30 6.5 31 11.0 32 17.0 33 24.0 34 25.5 35 29.5 36 54.5 37 69.0 38 76.0 39 90.5 40 108.0 41 134.0 42 144.0 43 152.5 44 168.5 45 167.0 46 170.0 47 186.0 48 185.0 49 168.5 50 164.5 51 156.5 52 151.5 53 138.0 54 113.5 55 112.5 56 104.5 57 101.0 58 86.0 59 62.5 60 69.0 61 75.5 62 66.0 63 67.0 64 75.5 65 62.0 66 54.0 67 56.5 68 51.5 69 41.5 70 40.5 71 35.5 72 20.5 73 19.5 74 25.0 75 24.5 76 12.5 77 3.5 78 2.5 79 3.5 80 3.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.8 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.525 #Duplication Level Percentage of deduplicated Percentage of total 1 73.50576721426076 52.575 2 18.03565186997553 25.8 3 5.627403005941979 12.075 4 1.8524991261796575 5.3 5 0.4893393918210416 1.7500000000000002 6 0.17476406850751486 0.75 7 0.13981125480601186 0.7000000000000001 8 0.10485844110450893 0.6 9 0.06990562740300593 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGATGCCCTCCCCTTTCGCCATGAGGTCGAAGGCCTTGTTGATCTCTGAG 9 0.22499999999999998 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATACGACCATCTCGTAT 9 0.22499999999999998 TruSeq Adapter, Index 27 (97% over 37bp) CTCGAGGTGGCGAAGGTCAATGGCCTGGCACAGAGCAATCAAGAACGTGG 8 0.2 No Hit GTTTCGTTCTTGTTTCAGCTTCTTCAAGCTTTTCCATCCACTTCTCAACA 8 0.2 No Hit GTCACCCCGAGGATGGGATCCTCCGGGCCCTGCGGGACGCCGGCGAAGAC 8 0.2 No Hit CAGCTTCAAAGCACCATCAACATACAAAAGATGTTCAAAAATCCAGTTTC 7 0.17500000000000002 No Hit TTCTTGGCTAATATGCGGTCAAGCAACTCACCACCCTCACATAATTCCAT 7 0.17500000000000002 No Hit GCAGAGGTTGAGCGGCACGGCGGCGAACTGGGTGGTGGCGCCGCCCTGGA 7 0.17500000000000002 No Hit CCGAGCTTGAGCTTGTAGAGGATGGTGGTCTTACCAGCGGCATCAAGACC 7 0.17500000000000002 No Hit GCCACTTTGACGCCTTGACAGACAGAAATGTAGTACAGCATCAGCATAAA 6 0.15 No Hit GCCGGATCTTGGAGGCGATCCCGAGGTGGCGACCTGGAGAGGTTCGTGCA 6 0.15 No Hit CCGTCTGTAGTGCCGAGATATCGCAAAAGTAGCATTCTTTCTCCAAAGAA 6 0.15 No Hit CAGGAGGTCTCTTCTGCATTTTACCTCTCAGCAAATTTCTCGATGCATGA 6 0.15 No Hit CCCGATGTACAATGGCCGTGTATGTATGTATGTATGTATAATTGAAGGAG 6 0.15 No Hit AGTGAGTTCACGTCCTGGTTGTGCTGCTCCGCGCTCTGGACGTGGTTGGT 5 0.125 No Hit CTCGAGGTGCCAAACCTTCCCGTCGATGTGGACTCTTGGGGAAGATCAGC 5 0.125 No Hit CGGGATTTGGCAGCGGCGAGGTCGCGGGAGCGGTGGAGGAGAGGCCTTGC 5 0.125 No Hit ATATAGATTGCTCTCCAAACAGGCTTCAACAAAACAAAATAAATCCAATA 5 0.125 No Hit GTTACATCACACTGGCAATGCTCGTAGAGAAATTCCCAGCTCTTACATGG 5 0.125 No Hit GGCCGAGAAGCTGTTCCAACTGCAGCAATCCTTCTTGCAAGCGCATCCAG 5 0.125 No Hit CCCATGGCTTCAAGATGAGCAGCAATCCTGGGGCACTTTTTAGCTAGAAG 5 0.125 No Hit GCATCATCCTGTCAGGGTACTCCTCTCGGATCTTGGAGATCAACAGTGTG 5 0.125 No Hit GTTCAGAACCAGTACTCATGATGATGAAGTCCGGCTTGTTGCCAGTTGAG 5 0.125 No Hit GCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCAC 5 0.125 No Hit GGGCTCCCCGTTTTCTCTGTGAACAGAGAGCAGCTCTTCTCTGAAGTTAT 5 0.125 No Hit GGACGGTGAAGGCCAGGGCGGTGGCGAAGCAGTAGGCGGCGAAGGCGGCG 5 0.125 No Hit CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGATGCCGGAGGCACGA 5 0.125 No Hit CAGAGTTGTAGTACAATACTGTTAACATCAAACGAACTCTTCAAAGTCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.1875 0.0 0.0 0.0 0.0 62-63 0.2375 0.0 0.0 0.0 0.0 64-65 0.2625 0.0 0.0 0.0 0.0 66-67 0.3 0.0 0.0 0.0 0.0 68-69 0.32499999999999996 0.0 0.0 0.0 0.0 70-71 0.45 0.0 0.0 0.0 0.0 72-73 0.5 0.0 0.0 0.0 0.0 74-75 0.5375 0.0 0.0 0.0 0.0 76-77 0.6499999999999999 0.0 0.0 0.0 0.0 78-79 0.9125000000000001 0.0 0.0 0.0 0.0 80-81 1.075 0.0 0.0 0.0 0.0 82-83 1.2999999999999998 0.0 0.0 0.0 0.0 84-85 1.425 0.0 0.0 0.0 0.0 86-87 1.725 0.0 0.0 0.0 0.0 88-89 2.025 0.0 0.0 0.0 0.0 90-91 2.4125 0.0 0.0 0.0 0.0 92-93 2.7375 0.0 0.0 0.0 0.0 94-95 3.0875000000000004 0.0 0.0 0.0 0.0 96-97 3.5125 0.0 0.0 0.0 0.0 98-99 3.9875 0.0 0.0 0.0 0.0 100-101 4.699999999999999 0.0 0.0 0.0 0.0 102-103 5.225 0.0 0.0 0.0 0.0 104-105 5.95 0.0 0.0 0.0 0.0 106-107 6.525 0.0 0.0 0.0 0.0 108-109 7.225 0.0 0.0 0.0 0.0 110-111 7.9 0.0 0.0 0.0 0.0 112-113 8.537500000000001 0.0 0.0 0.0 0.0 114-115 9.2125 0.0 0.0 0.0 0.0 116-117 9.7375 0.0 0.0 0.0 0.0 118-119 10.55 0.0 0.0 0.0 0.0 120-121 11.425 0.0 0.0 0.0 0.0 122-123 12.0125 0.0 0.0 0.0 0.0 124-125 12.75 0.0 0.0 0.0 0.0 126-127 13.9 0.0 0.0 0.0 0.0 128-129 14.9875 0.0 0.0 0.0 0.0 130-131 15.8625 0.0 0.0 0.0 0.0 132-133 16.6 0.0 0.0 0.0 0.0 134-135 17.35 0.0 0.0 0.0 0.0 136-137 18.275 0.0 0.0 0.0 0.0 138-139 19.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAAA 10 0.006830828 145.0 9 ATGATCC 10 0.006830828 145.0 145 CTCTTTT 10 0.006830828 145.0 145 GCAAACG 10 0.006830828 145.0 6 CCCTCTG 10 0.006830828 145.0 3 AAACGAA 10 0.006830828 145.0 8 CAAACGA 10 0.006830828 145.0 7 GGCAGGC 10 0.006830828 145.0 6 AAGCAAA 10 0.006830828 145.0 4 >>END_MODULE SRR13165352 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165352_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0505 37.0 37.0 37.0 37.0 37.0 2 36.2475 37.0 37.0 37.0 37.0 37.0 3 36.318 37.0 37.0 37.0 37.0 37.0 4 36.2495 37.0 37.0 37.0 37.0 37.0 5 36.374 37.0 37.0 37.0 37.0 37.0 6 36.4015 37.0 37.0 37.0 37.0 37.0 7 36.3375 37.0 37.0 37.0 37.0 37.0 8 36.2945 37.0 37.0 37.0 37.0 37.0 9 36.298 37.0 37.0 37.0 37.0 37.0 10-14 36.310500000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.2473 37.0 37.0 37.0 37.0 37.0 20-24 36.22025 37.0 37.0 37.0 37.0 37.0 25-29 36.173849999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.146750000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.17145 37.0 37.0 37.0 37.0 37.0 40-44 36.128550000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.0892 37.0 37.0 37.0 37.0 37.0 50-54 36.059749999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.02315 37.0 37.0 37.0 37.0 37.0 60-64 36.00705000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.01219999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.940149999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.92485 37.0 37.0 37.0 37.0 37.0 80-84 35.9894 37.0 37.0 37.0 37.0 37.0 85-89 35.851350000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.84565 37.0 37.0 37.0 37.0 37.0 95-99 35.86055 37.0 37.0 37.0 37.0 37.0 100-104 35.90915 37.0 37.0 37.0 37.0 37.0 105-109 35.814750000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.73705 37.0 37.0 37.0 37.0 37.0 115-119 35.73345 37.0 37.0 37.0 37.0 37.0 120-124 35.60845 37.0 37.0 37.0 37.0 37.0 125-129 35.5614 37.0 37.0 37.0 37.0 37.0 130-134 35.35455 37.0 37.0 37.0 37.0 37.0 135-139 35.1983 37.0 37.0 37.0 32.2 37.0 140-144 35.062650000000005 37.0 37.0 37.0 32.2 37.0 145-149 34.708549999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.377875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 3.0 15 4.0 16 1.0 17 1.0 18 2.0 19 2.0 20 2.0 21 7.0 22 13.0 23 12.0 24 9.0 25 10.0 26 5.0 27 8.0 28 13.0 29 11.0 30 26.0 31 30.0 32 69.0 33 100.0 34 215.0 35 444.0 36 2714.0 37 294.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.54705586311022 20.130850528434827 8.75691997986915 21.565173628585807 2 30.625000000000004 22.875 24.349999999999998 22.15 3 23.175 24.575 29.325000000000003 22.925 4 26.650000000000002 29.425 18.75 25.174999999999997 5 29.225 31.674999999999997 18.55 20.549999999999997 6 23.9 35.35 18.275 22.475 7 22.25 21.675 31.05 25.025 8 23.9 24.275 22.25 29.575000000000003 9 24.825 21.175 27.375 26.625 10-14 27.0 25.19 22.509999999999998 25.3 15-19 26.3 25.105 23.674999999999997 24.92 20-24 26.161308065403272 24.636231811590577 24.421221061053053 24.781239061953098 25-29 26.39659914978745 24.381095273818453 23.280820205051263 25.941485371342836 30-34 25.713857078561787 24.8887333099965 24.258638795819373 25.13877081562234 35-39 26.146307315365767 24.401220061003052 24.31621581079054 25.136256812840642 40-44 26.583987598139718 24.763714557183576 23.758563784567684 24.893734060109015 45-49 26.45764576457646 24.307430743074306 23.72737273727373 25.50755075507551 50-54 26.151307565378268 24.661233061653082 24.046202310115504 25.141257062853146 55-59 25.566391597899475 24.836209052263065 24.07101775443861 25.52638159539885 60-64 26.491324566228315 24.306215310765538 24.516225811290564 24.686234311715584 65-69 26.815 24.995 23.435 24.755 70-74 26.346586646661663 24.616154038509627 23.950987746936732 25.08627156789197 75-79 27.089063359503925 24.77871680752113 24.143621543231486 23.98859828974346 80-84 25.629999999999995 25.405 23.95 25.014999999999997 85-89 27.7569392348087 24.641160290072516 24.021005251312825 23.58089522380595 90-94 27.011350567528375 24.141207060353018 24.72123606180309 24.126206310315514 95-99 26.851342567128356 25.29126456322816 23.35116755837792 24.506225311265563 100-104 26.721680420105027 25.301325331332837 23.615903975993998 24.36109027256814 105-109 27.09177294323581 25.681420355088775 23.385846461615404 23.840960240060017 110-114 28.286414320716034 24.756237811890593 23.631181559077955 23.326166308315415 115-119 28.11702925731433 24.576144036009 23.29082270567642 24.016004001000248 120-124 27.851962990747687 25.641410352588146 23.145786446611652 23.360840210052515 125-129 28.21564312862572 26.16523304660932 22.569513902780557 23.049609921984395 130-134 29.225228830090533 25.62897013954884 22.57790226579303 22.567898764567598 135-139 29.633890167050115 25.627688306491947 23.357007102130638 21.381414424327296 140-144 30.111505575278763 24.946247312365617 22.88114405720286 22.06110305515276 145-149 30.857714428607153 25.06626656664166 23.05076269067267 21.02525631407852 150-151 31.711891959484806 24.58421908215581 22.24584219082156 21.458046767537827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.0 20 1.0 21 0.5 22 0.0 23 1.5 24 2.0 25 2.0 26 4.5 27 4.5 28 1.5 29 0.5 30 1.5 31 3.5 32 5.0 33 12.5 34 23.5 35 27.5 36 39.0 37 48.0 38 60.0 39 78.5 40 93.0 41 120.5 42 145.5 43 160.5 44 179.5 45 184.5 46 183.0 47 181.5 48 183.5 49 168.0 50 139.5 51 137.5 52 128.5 53 131.5 54 133.0 55 109.5 56 98.0 57 90.0 58 91.0 59 89.0 60 75.0 61 78.5 62 85.0 63 69.5 64 62.5 65 69.5 66 73.5 67 67.0 68 56.5 69 49.0 70 41.5 71 39.5 72 28.0 73 23.5 74 23.5 75 18.0 76 13.0 77 11.0 78 13.5 79 8.5 80 3.0 81 2.5 82 2.5 83 2.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.5 94 1.0 95 1.5 96 1.0 97 0.0 98 0.5 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.65 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.025 90-94 0.005 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.034999999999999996 135-139 0.03 140-144 0.005 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.45 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84472049689441 54.225 2 17.184265010351968 24.9 3 5.106970324361629 11.1 4 1.8978605935127675 5.5 5 0.5175983436853002 1.875 6 0.10351966873706005 0.44999999999999996 7 0.10351966873706005 0.525 8 0.2070393374741201 1.2 9 0.03450655624568668 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAATCCCAGGTGTCTTTGAGATGGTCTGGAGCCCATTCACGTTTCTTCT 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 8 0.2 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 8 0.2 No Hit AGCAACATGAACGACCTGGTTGCTGAGTATCAGCAGTACCAGGACGCCAC 8 0.2 No Hit CGCCATGGCTTCCTACTGCTCTGAGCTGCAGTTCTTGGGCAACCCGGTGA 8 0.2 No Hit GGTGGTTGACCGCAGTGTCGAATGCACTGGCAACGTCAACGCCATGATAC 8 0.2 No Hit CAGCAACAACAACAACCGCCACCTCCGCCGACTCATCAGCCTCCCTCCGA 8 0.2 No Hit CAGCCACAACCTCTCGCGTTGATTCGAGCGGTTCCAAGCATCAAACCCCC 7 0.17500000000000002 No Hit GTTCGACGCCGCCATCAAGAAGGCCGAGGCCGACCTCCGCGCGCTGCTCG 7 0.17500000000000002 No Hit TTTCGAGGCGCGGTATACGATCGGGAAGCTTCTTGGCCACGGACAGTTCG 7 0.17500000000000002 No Hit GATCTGCTGGAGAAGGGTTAGTCTAGCTAACATCGTACAGTGAAGGTCTG 6 0.15 No Hit ATTGTGCTGTGCCTTCTAGAGTCCATTTTTTGTCTGCTATTGTCCTCTCC 6 0.15 No Hit GTCAGTATGGCGTCCGGAGCATACCAACCATGATGATATTCAAGAATGGT 6 0.15 No Hit AAATTACGTAGCTGCTCTGTTGTTGCTCGTTATGAAGACAGAGGAAGATG 5 0.125 No Hit GAACTCTTGAGGTCTTCGTCTTAACCGCAAATTTTTCATTTCCCACACAC 5 0.125 No Hit ACTATCTGATGTTACCTGTGAATGCACCGAAATGTGCTCACCACAACAAC 5 0.125 No Hit AAATGATGTTGGTGGAAGGCTGCTTCTTCAGAAACATTTGCCTGTAGGAT 5 0.125 No Hit AGTCGGAGACGAAGAAGCAGGAGGCTCAGAAGCCGCGGGGCGCGCACCGA 5 0.125 No Hit GAAATCCAGAGATTTTCCCACTCTGATGTTGTCGAAGTCTATGGATAGCT 5 0.125 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 5 0.125 No Hit CCAACACCGGGAGGCCAACTCCGCAAGCTCCAATGACACATCCATGGACA 5 0.125 No Hit GGCGATCCACGGTGGTAACTTCCAGGGCACCCCCATCGGTGTCTCCATGG 5 0.125 No Hit CCGGCGCAGCGGCCGCTGGTTTTGGGCTCCGGCACGCCGATGAGGAGCAT 5 0.125 No Hit GGTCCGACCCATCAACCCATTGAGCACTTGGTGAGCTTCCGTGCAATGCC 5 0.125 No Hit GCTCGTAGTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGC 5 0.125 No Hit GTACCATGGACAGCGTCCGCACCGGGCCCTACGGCCAGATCTTCCGCCCC 5 0.125 No Hit GGAAATAATTGCAACAGCAGCAGAAGCAGAAGCTTCTTCTGTAGCAAACT 5 0.125 No Hit TAGTTGTAGCTTCTGCTATTTTCGGGAATTATGATATGATTCAACATCCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.1875 0.0 0.0 0.0 0.0 62-63 0.2375 0.0 0.0 0.0 0.0 64-65 0.2625 0.0 0.0 0.0 0.0 66-67 0.3 0.0 0.0 0.0 0.0 68-69 0.32499999999999996 0.0 0.0 0.0 0.0 70-71 0.45 0.0 0.0 0.0 0.0 72-73 0.5 0.0 0.0 0.0 0.0 74-75 0.5375 0.0 0.0 0.0 0.0 76-77 0.6499999999999999 0.0 0.0 0.0 0.0 78-79 0.9125000000000001 0.0 0.0 0.0 0.0 80-81 1.025 0.0 0.0 0.0 0.0 82-83 1.25 0.0 0.0 0.0 0.0 84-85 1.3625 0.0 0.0 0.0 0.0 86-87 1.65 0.0 0.0 0.0 0.0 88-89 1.95 0.0 0.0 0.0 0.0 90-91 2.3375 0.0 0.0 0.0 0.0 92-93 2.6624999999999996 0.0 0.0 0.0 0.0 94-95 3.0 0.0 0.0 0.0 0.0 96-97 3.4124999999999996 0.0 0.0 0.0 0.0 98-99 3.8875 0.0 0.0 0.0 0.0 100-101 4.6 0.0 0.0 0.0 0.0 102-103 5.137499999999999 0.0 0.0 0.0 0.0 104-105 5.875 0.0 0.0 0.0 0.0 106-107 6.45 0.0 0.0 0.0 0.0 108-109 7.15 0.0 0.0 0.0 0.0 110-111 7.8125 0.0 0.0 0.0 0.0 112-113 8.4375 0.0 0.0 0.0 0.0 114-115 9.1125 0.0 0.0 0.0 0.0 116-117 9.649999999999999 0.0 0.0 0.0 0.0 118-119 10.475000000000001 0.0 0.0 0.0 0.0 120-121 11.35 0.0 0.0 0.0 0.0 122-123 11.9375 0.0 0.0 0.0 0.0 124-125 12.6875 0.0 0.0 0.0 0.0 126-127 13.837499999999999 0.0 0.0 0.0 0.0 128-129 14.875 0.0 0.0 0.0 0.0 130-131 15.7625 0.0 0.0 0.0 0.0 132-133 16.475 0.0 0.0 0.0 0.0 134-135 17.2125 0.0 0.0 0.0 0.0 136-137 18.15 0.0 0.0 0.0 0.0 138-139 18.875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCCCT 10 0.006830828 145.0 9 TGAGCGG 10 0.006830828 145.0 145 >>END_MODULE Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329732 spots for SRR13165352.sra Written 1329732 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra Read 1329714 spots for SRR13165352.sra Written 1329714 spots for SRR13165352.sra SRR ids: ['SRR13165352.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_sa5n73xm SRR13165352.sra spots: 26594298 blocks: [[1, 1329714], [1329715, 2659428], [2659429, 3989142], [3989143, 5318856], [5318857, 6648570], [6648571, 7978284], [7978285, 9307998], [9307999, 10637712], [10637713, 11967426], [11967427, 13297140], [13297141, 14626854], [14626855, 15956568], [15956569, 17286282], [17286283, 18615996], [18615997, 19945710], [19945711, 21275424], [21275425, 22605138], [22605139, 23934852], [23934853, 25264566], [25264567, 26594298]] SRR13165352 file size 9016205 SRR13165352 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165352 SRR13165352_1.fastq SRR13165352_2.fastq Input file: SRR13165352_1.fastq Paired file: SRR13165352_2.fastq trimmed: SRR13165352-trimmed-pair1.fastq, SRR13165352-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 15:50:51 2024 >> started Sat Dec 7 15:51:18 2024 >> done (27.858s) 26594298 read pairs processed; of these: 683 ( 0.00%) short read pairs filtered out after trimming by size control 64055 ( 0.24%) empty read pairs filtered out after trimming by size control 26529560 (99.76%) read pairs available; of these: 6138629 (23.14%) trimmed read pairs available after processing 20390931 (76.86%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 28 0.00% 19 31 0.00% 20 37 0.00% 21 52 0.00% 22 69 0.00% 23 98 0.00% 24 99 0.00% 25 121 0.00% 26 117 0.00% 27 142 0.00% 28 154 0.00% 29 211 0.00% 30 190 0.00% 31 193 0.00% 32 202 0.00% 33 234 0.00% 34 214 0.00% 35 222 0.00% 36 311 0.00% 37 299 0.00% 38 304 0.00% 39 329 0.00% 40 388 0.00% 41 450 0.00% 42 479 0.00% 43 467 0.00% 44 515 0.00% 45 540 0.00% 46 567 0.00% 47 668 0.00% 48 833 0.00% 49 820 0.00% 50 1010 0.00% 51 1096 0.00% 52 1186 0.00% 53 1311 0.00% 54 1379 0.01% 55 1454 0.01% 56 1646 0.01% 57 1802 0.01% 58 2091 0.01% 59 2398 0.01% 60 2688 0.01% 61 3380 0.01% 62 3655 0.01% 63 3934 0.01% 64 4273 0.02% 65 4848 0.02% 66 5200 0.02% 67 5611 0.02% 68 6158 0.02% 69 6999 0.03% 70 7994 0.03% 71 8722 0.03% 72 10168 0.04% 73 11395 0.04% 74 12897 0.05% 75 13976 0.05% 76 14591 0.05% 77 15752 0.06% 78 17664 0.07% 79 19463 0.07% 80 21281 0.08% 81 23321 0.09% 82 25660 0.10% 83 28366 0.11% 84 30975 0.12% 85 33337 0.13% 86 34927 0.13% 87 37147 0.14% 88 38756 0.15% 89 40165 0.15% 90 43630 0.16% 91 45954 0.17% 92 48541 0.18% 93 52395 0.20% 94 55962 0.21% 95 57464 0.22% 96 60466 0.23% 97 62577 0.24% 98 63178 0.24% 99 65376 0.25% 100 66928 0.25% 101 69169 0.26% 102 71804 0.27% 103 73739 0.28% 104 77010 0.29% 105 79155 0.30% 106 80250 0.30% 107 81624 0.31% 108 83144 0.31% 109 84088 0.32% 110 83949 0.32% 111 86421 0.33% 112 89423 0.34% 113 89731 0.34% 114 92290 0.35% 115 94656 0.36% 116 96301 0.36% 117 97143 0.37% 118 96993 0.37% 119 97532 0.37% 120 98436 0.37% 121 99480 0.37% 122 99757 0.38% 123 101870 0.38% 124 103200 0.39% 125 104415 0.39% 126 105923 0.40% 127 106178 0.40% 128 106031 0.40% 129 107300 0.40% 130 106826 0.40% 131 106616 0.40% 132 108249 0.41% 133 108075 0.41% 134 108913 0.41% 135 109814 0.41% 136 111253 0.42% 137 109841 0.41% 138 110600 0.42% 139 111958 0.42% 140 110574 0.42% 141 111330 0.42% 142 113024 0.43% 143 111759 0.42% 144 113191 0.43% 145 115422 0.44% 146 115066 0.43% 147 116664 0.44% 148 113452 0.43% 149 114272 0.43% 150 114187 0.43% 151 20390931 76.86% 26529560 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=3.39 fanout-score-rank=25 prefix-density=0.33 prefix-fanout=2.5 sequence=TCCTGGATCTTGGCCTTCACGTTGTCGATGGTGTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=24 fanout-score=99.67 fanout-score-rank=1 prefix-density=0.46 prefix-fanout=18.2 sequence=CGGCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAA criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=5.97 fanout-score-rank=18 prefix-density=0.35 prefix-fanout=3.9 sequence=GACAATGAGGCC criterion=fanout-score sequence-density=0.13 sequence-density-rank=18 fanout-score=124.29 fanout-score-rank=1 prefix-density=0.73 prefix-fanout=21.8 sequence=GCTGCTGCTCAGGCCTCAAGGAAATCATCTCCCAGAGTGAATAATGAGGAAGTTCAGAAGGCTGCGGCTGCTTTGAAGGGCTCTGACCACCGACGTGCTACCACTGTTTCTGCTAGACTGGACGCTCAGCAGAAAAAGCTCAACCTACCTGTCCTTCCAACAACCACAATTGGTTCATT SRR13165352 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 15:52:11 Started mapping on | Dec 07 15:52:11 Finished on | Dec 07 15:54:14 Mapping speed, Million of reads per hour | 776.47 Number of input reads | 26529560 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 24694604 Uniquely mapped reads % | 93.08% Average mapped length | 286.42 Number of splices: Total | 21437238 Number of splices: Annotated (sjdb) | 20146142 Number of splices: GT/AG | 21168385 Number of splices: GC/AG | 221668 Number of splices: AT/AC | 11313 Number of splices: Non-canonical | 35872 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.02% Deletion average length | 2.91 Insertion rate per base | 0.01% Insertion average length | 2.24 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 239455 % of reads mapped to multiple loci | 0.90% Number of reads mapped to too many loci | 134045 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.22% % of reads unmapped: other | 2.29% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1595792 1595792 1595792 N_multimapping 239455 239455 239455 N_noFeature 803902 23996561 1059659 N_ambiguous 498702 3231 57584 UnstrandedReadsAssigned:23392000 PositiveStrandReadsAssigned:694812 NegativeStrandReadsAssigned:23577361 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=144 echo kmer=139 SRR13165352 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165352-trimmed-pair1.fastq SRR13165352-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 26,529,560 reads, 23,902,313 reads pseudoaligned [quant] estimated average fragment length: 230.285 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,354 rounds 52973 SRR13165352.ke.tsv 35125 SRR13165352.se.tsv 88098 total ==> SRR13165352.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 707.285 0 0 PNS24247 1044 814.715 57.8659 4.73461 PNS24249 1928 1698.71 85.2561 3.34559 PNS24246 1044 814.715 57.8659 4.73461 PNS24248 1044 814.715 57.8659 4.73461 PNS24244 1471 1241.71 129.146 6.9331 PNS24243 293 115.96 1 0.574856 KQK14069 1603 1373.71 36 1.74692 KQK14071 474 262.046 0 0 ==> SRR13165352.se.tsv <== BRADI_1g14170v3 37 BRADI_1g53295v3 177 BRADI_1g59795v3 410 BRADI_1g07683v3 0 BRADI_1g00485v3 14 BRADI_1g20270v3 512 BRADI_1g74790v3 496 BRADI_1g09890v3 8 BRADI_1g77505v3 344 BRADI_1g48960v3 0 SRR13165352 completed mapping pipeline successfully