Starting /dee2/code/volunteer_pipeline.sh SRR13165354 current disk space = 1542197604352 free memory = 1475892024 SRR13165354 SRAfilesize 434e707af370f9548d51aeb7a56502b2 SRR13165354.sra SRR13165354.sra file validated SRR13165354 is paired end SRR13165354 is conventional basespace SRR13165354 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165354_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.589 37.0 37.0 37.0 37.0 37.0 2 36.05725 37.0 37.0 37.0 37.0 37.0 3 36.486 37.0 37.0 37.0 37.0 37.0 4 36.5375 37.0 37.0 37.0 37.0 37.0 5 36.476 37.0 37.0 37.0 37.0 37.0 6 36.6005 37.0 37.0 37.0 37.0 37.0 7 36.427 37.0 37.0 37.0 37.0 37.0 8 36.661 37.0 37.0 37.0 37.0 37.0 9 36.3725 37.0 37.0 37.0 37.0 37.0 10-14 36.5338 37.0 37.0 37.0 37.0 37.0 15-19 36.4551 37.0 37.0 37.0 37.0 37.0 20-24 36.4645 37.0 37.0 37.0 37.0 37.0 25-29 36.355900000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.3724 37.0 37.0 37.0 37.0 37.0 35-39 36.3366 37.0 37.0 37.0 37.0 37.0 40-44 36.3163 37.0 37.0 37.0 37.0 37.0 45-49 36.278 37.0 37.0 37.0 37.0 37.0 50-54 36.2672 37.0 37.0 37.0 37.0 37.0 55-59 36.27890000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.3206 37.0 37.0 37.0 37.0 37.0 65-69 36.249199999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.251099999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.2587 37.0 37.0 37.0 37.0 37.0 80-84 36.1774 37.0 37.0 37.0 37.0 37.0 85-89 36.1826 37.0 37.0 37.0 37.0 37.0 90-94 36.1019 37.0 37.0 37.0 37.0 37.0 95-99 36.0577 37.0 37.0 37.0 37.0 37.0 100-104 36.1342 37.0 37.0 37.0 37.0 37.0 105-109 36.126 37.0 37.0 37.0 37.0 37.0 110-114 35.9605 37.0 37.0 37.0 37.0 37.0 115-119 36.068400000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.9621 37.0 37.0 37.0 37.0 37.0 125-129 35.917899999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.811 37.0 37.0 37.0 37.0 37.0 135-139 35.676100000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.4716 37.0 37.0 37.0 37.0 37.0 145-149 35.211400000000005 37.0 37.0 37.0 37.0 37.0 150-151 34.993 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 0.0 25 3.0 26 7.0 27 9.0 28 17.0 29 28.0 30 30.0 31 38.0 32 68.0 33 91.0 34 171.0 35 350.0 36 2826.0 37 359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.7 9.925 4.375 30.0 2 22.23342576254096 10.234434081169649 34.56012099823545 32.972019158053946 3 19.650000000000002 14.7 26.025 39.625 4 25.474999999999998 20.925 23.599999999999998 30.0 5 27.825 25.55 23.575 23.05 6 24.175 29.95 22.375 23.5 7 19.925 23.150000000000002 39.050000000000004 17.875 8 20.424999999999997 22.85 30.075000000000003 26.650000000000002 9 19.875 20.849999999999998 34.375 24.9 10-14 23.075000000000003 26.240000000000002 26.465 24.22 15-19 23.265 24.65 26.619999999999997 25.465 20-24 23.875 24.52 26.090000000000003 25.515 25-29 22.57 25.345000000000002 26.435 25.650000000000002 30-34 23.29 24.895 26.119999999999997 25.695 35-39 24.240000000000002 25.095 25.290000000000003 25.374999999999996 40-44 23.0 24.845 25.985000000000003 26.169999999999998 45-49 23.435 25.845000000000002 25.685000000000002 25.035 50-54 23.855 24.185000000000002 26.33 25.629999999999995 55-59 23.935000000000002 25.895000000000003 24.82 25.35 60-64 22.875 25.155 26.14 25.83 65-69 23.76 24.67 26.334999999999997 25.235000000000003 70-74 24.2 24.755 25.655 25.39 75-79 23.82 25.569999999999997 25.580000000000002 25.03 80-84 24.335 24.895 26.090000000000003 24.68 85-89 24.73 24.77 24.959999999999997 25.540000000000003 90-94 23.41 24.925 25.3 26.365 95-99 24.240000000000002 24.82 25.35 25.590000000000003 100-104 24.335 25.41 25.39 24.865000000000002 105-109 23.5 25.314999999999998 25.435000000000002 25.75 110-114 23.61 25.014999999999997 24.945 26.43 115-119 24.005000000000003 25.09 25.035 25.869999999999997 120-124 24.07 24.740000000000002 25.724999999999998 25.465 125-129 23.830000000000002 25.83 24.224999999999998 26.115 130-134 23.06 25.825 24.610000000000003 26.505000000000003 135-139 24.365000000000002 25.365 24.645 25.624999999999996 140-144 24.345 24.55 25.2 25.905 145-149 24.404999999999998 24.529999999999998 25.5 25.564999999999998 150-151 23.325000000000003 24.7875 24.25 27.6375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 0.5 26 4.0 27 6.0 28 2.5 29 2.5 30 6.5 31 9.5 32 10.5 33 9.5 34 24.5 35 41.0 36 45.0 37 51.0 38 74.5 39 99.0 40 116.0 41 146.0 42 166.0 43 168.5 44 182.5 45 206.0 46 208.5 47 206.5 48 200.0 49 196.5 50 173.0 51 147.0 52 132.5 53 110.0 54 108.5 55 105.5 56 115.5 57 104.0 58 76.0 59 80.0 60 86.5 61 71.5 62 53.5 63 54.5 64 57.0 65 47.0 66 49.5 67 58.0 68 43.0 69 36.0 70 27.0 71 17.5 72 18.0 73 16.5 74 16.0 75 7.0 76 2.0 77 1.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.8250000000000001 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.075 #Duplication Level Percentage of deduplicated Percentage of total 1 74.81789802289282 53.925 2 16.857440166493237 24.3 3 5.272285813388831 11.4 4 1.6996184530003469 4.9 5 0.72840790842872 2.625 6 0.4856052722858134 2.1 7 0.10405827263267431 0.525 8 0.0 0.0 9 0.0346860908775581 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGAAATGCTGGGTTTGATGTGATCTAGTATTCTTCAGCAGCGCTAGCAG 9 0.22499999999999998 No Hit GACGCCGGTGCACGTTTCGGCGGCGGCCTGGCTCTTGTAGCCGATGACGG 7 0.17500000000000002 No Hit CACCACGCTGAATCCATCTTCTTGGCCCTTTCATACCTTCAGGGCCAGCT 7 0.17500000000000002 No Hit CTTTCTTCGACAGCGTAAACTGTCCTGATCAAGCAACAACTTGTTTGACA 7 0.17500000000000002 No Hit CCTTGACGGCCTTATCACCCTGACCGACTGCGTCGAACACGACATCGTAC 6 0.15 No Hit GCCCTGGCTGATGTACTCTTGGGAGCTGAGGACGGCCACGTGGGTACCGT 6 0.15 No Hit CGGGCCGTTGGGGATGCTGACGACAGTGCGCCACTTGGCGAAGCGGGCGC 6 0.15 No Hit ATGCCGATAAAAATGCCCTGGAATTTAATATTTAGCAGAGCAACTTCATG 6 0.15 No Hit GTTCACGTTATCAGAGGCATGTTGCTCAATGTTGCGTATCCAGTTCCGTA 6 0.15 No Hit ATGCTGCCTTACCTAAGTTCATGTTTTTCTTCGTCAGAAAATACAATGAA 6 0.15 No Hit CTTCCCTTCCGATGAGCGCCTCGTCGAAGCTGCTTGCCTTCTTGTTCCTC 6 0.15 No Hit GTCCCATCTCAACCCCACCATGTGTTACCAGGACAGTGCCATCAGTATAA 6 0.15 No Hit GTGCTTTTTTCAACCATTATCCACAGCTTCATCAAGGGAACCAAACAAAT 6 0.15 No Hit GAGGGTTTTCACATATGCGATCTTTTTTTCAACACTTATTGCTGTTTCAT 6 0.15 No Hit GCCACATCAGTTGCCTCGGCACGAGTAGGCCTGAGGTTGTCAGTCATACT 6 0.15 No Hit GCCGTTTCCCTTCTTCACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGC 6 0.15 No Hit ATGATCTTGTCGAGCAGCTCGTGCCACCTCTTCTTCAGCGCGATCGTCCT 6 0.15 No Hit GGAGGCCAGCCCGCCCTGCCGGGATATGTACTGGAAGGCGTTGTCCATCA 6 0.15 No Hit ATGGCCTGTCTCATAAGCCTACAGCCCAAAACTTGGATTCTTGGAGGCAT 5 0.125 No Hit GTCGGAGACAGCGGGGCAGTCGTTACACCATTCGTGCAGGTCGCTACTTA 5 0.125 No Hit CTTCATTCCTAATGGTTGGTATTACAACACCATTCCTGGCGACTTCATCA 5 0.125 No Hit GCCGTGGCCAAATCTTTTCCTCTATTCAGTAATCGGTTCTATAAATAATG 5 0.125 No Hit GTCCGCTACACCAATATAGAAGGCCTCGGTGTCTGGGCTAAATGACAAAC 5 0.125 No Hit GGGGAATACTGCTTTGGATCCCTCTTCTGCAATGCGTGCTTTGAGTTGCT 5 0.125 No Hit CTTCAAATAATTGCAAAGACTCTTCAATAGGCCTATTCCTCCATGGACTA 5 0.125 No Hit GTAGAGGGTCTCCTCGAAGAGGATAGCACCAGAGATGTAATTTCCCAGGC 5 0.125 No Hit CTCAGCTCTAATTTTCTTCTCCTTGTTAAGAACAGCATCTGGATGAGTAT 5 0.125 No Hit GGTCGTTGCATGCGTCCCTGGCATGCAAATTAAGCTGCTGCAGCAGATTG 5 0.125 No Hit ACCACCAATGTAACCACCAATCCTAGAAGTCATCTTTGCACAGCCCTGGA 5 0.125 No Hit GTCACGTTCATTGGTGTTTTTCCCCTTCTGTTTCTTCTTCAACGGAGATT 5 0.125 No Hit GAGTGCGCCAGCCAGGTGGAAGAGGAGGGGAGCGGGTCGTGCAGTGGCGG 5 0.125 No Hit GTTCGAGTGTGTGTGTGTGTGTGAAATCTGGAGAATTTTCGGTGATCGAC 5 0.125 No Hit GCCTGGGCCGCTGCTGCCGCCGGCAGTGCTCGCCGGAGTGTCCTCGTCCA 5 0.125 No Hit GCTCTATGCCTCTCCATTGTCGTCTCGAAACCATTGTTATTGCTAGACCT 5 0.125 No Hit CTTCCATCTGTAACAACTCTTGATTCTTCTCCTTCTTCTTCTTTGCCTGG 5 0.125 No Hit CTCACGGAACACAAAGCCGACCTTGCTGAACTCAAGGCAAGGAACCCACT 5 0.125 No Hit CCCAAGGCCAATCTTCTTGTCAAGAATGAACCCTTCATCCAAAAAGGAAT 5 0.125 No Hit GCACACAGTAACAGAAAAGGATAAAAAATCTGGCACTTGCTATGAAGAGT 5 0.125 No Hit CCCAGACTCGATGTTCATCATCACCATTGTCTTCCGACACGACACGACCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.23750000000000002 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.36250000000000004 0.0 0.0 0.0 0.0 80-81 0.5125 0.0 0.0 0.0 0.0 82-83 0.7 0.0 0.0 0.0 0.0 84-85 0.875 0.0 0.0 0.0 0.0 86-87 1.0375 0.0 0.0 0.0 0.0 88-89 1.2625000000000002 0.0 0.0 0.0 0.0 90-91 1.5125000000000002 0.0 0.0 0.0 0.0 92-93 1.7375 0.0 0.0 0.0 0.0 94-95 2.0999999999999996 0.0 0.0 0.0 0.0 96-97 2.35 0.0 0.0 0.0 0.0 98-99 2.675 0.0 0.0 0.0 0.0 100-101 3.0125 0.0 0.0 0.0 0.0 102-103 3.4 0.0 0.0 0.0 0.0 104-105 3.725 0.0 0.0 0.0 0.0 106-107 4.2125 0.0 0.0 0.0 0.0 108-109 4.487500000000001 0.0 0.0 0.0 0.0 110-111 5.0 0.0 0.0 0.0 0.0 112-113 5.4 0.0 0.0 0.0 0.0 114-115 5.9125 0.0 0.0 0.0 0.0 116-117 6.6 0.0 0.0 0.0 0.0 118-119 7.575 0.0 0.0 0.0 0.0 120-121 8.024999999999999 0.0 0.0 0.0 0.0 122-123 8.6125 0.0 0.0 0.0 0.0 124-125 9.600000000000001 0.0 0.0 0.0 0.0 126-127 10.149999999999999 0.0 0.0 0.0 0.0 128-129 11.075 0.0 0.0 0.0 0.0 130-131 11.8875 0.0 0.0 0.0 0.0 132-133 12.8875 0.0 0.0 0.0 0.0 134-135 13.6125 0.0 0.0 0.0 0.0 136-137 14.3125 0.0 0.0 0.0 0.0 138-139 14.875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTGAT 10 0.006830828 145.0 6 CTGATGT 10 0.006830828 145.0 8 TAATTTT 10 0.006830828 145.0 9 TGATGTA 10 0.006830828 145.0 9 >>END_MODULE SRR13165354 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165354_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.89525 37.0 37.0 37.0 37.0 37.0 2 36.0415 37.0 37.0 37.0 37.0 37.0 3 36.047 37.0 37.0 37.0 37.0 37.0 4 36.2435 37.0 37.0 37.0 37.0 37.0 5 36.148 37.0 37.0 37.0 37.0 37.0 6 36.2315 37.0 37.0 37.0 37.0 37.0 7 36.25 37.0 37.0 37.0 37.0 37.0 8 36.33 37.0 37.0 37.0 37.0 37.0 9 36.2235 37.0 37.0 37.0 37.0 37.0 10-14 36.214200000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.1434 37.0 37.0 37.0 37.0 37.0 20-24 36.16245 37.0 37.0 37.0 37.0 37.0 25-29 36.040350000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.08375 37.0 37.0 37.0 37.0 37.0 35-39 36.01775 37.0 37.0 37.0 37.0 37.0 40-44 36.01125 37.0 37.0 37.0 37.0 37.0 45-49 36.02755 37.0 37.0 37.0 37.0 37.0 50-54 35.94775 37.0 37.0 37.0 37.0 37.0 55-59 35.973349999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.902249999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.9286 37.0 37.0 37.0 37.0 37.0 70-74 35.840050000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.86215 37.0 37.0 37.0 37.0 37.0 80-84 35.88655 37.0 37.0 37.0 37.0 37.0 85-89 35.74065 37.0 37.0 37.0 37.0 37.0 90-94 35.70675 37.0 37.0 37.0 37.0 37.0 95-99 35.730250000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.73325 37.0 37.0 37.0 37.0 37.0 105-109 35.634249999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.58585000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.60985 37.0 37.0 37.0 37.0 37.0 120-124 35.54905000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.447649999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.30845000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.22185 37.0 37.0 37.0 34.6 37.0 140-144 35.087450000000004 37.0 37.0 37.0 32.2 37.0 145-149 34.987 37.0 37.0 37.0 27.4 37.0 150-151 34.701 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 7.0 14 4.0 15 6.0 16 0.0 17 1.0 18 3.0 19 3.0 20 4.0 21 8.0 22 4.0 23 6.0 24 10.0 25 6.0 26 8.0 27 8.0 28 15.0 29 16.0 30 19.0 31 41.0 32 78.0 33 107.0 34 214.0 35 545.0 36 2649.0 37 237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.56603773584906 21.635220125786166 6.9937106918239 24.80503144654088 2 30.65 24.85 24.325 20.175 3 25.0 23.75 28.975 22.275 4 27.700000000000003 30.825000000000003 20.25 21.224999999999998 5 29.349999999999998 33.5 19.075 18.075 6 21.775 38.074999999999996 18.6 21.55 7 21.125 19.775000000000002 35.35 23.75 8 22.575 24.474999999999998 24.025 28.925 9 25.575 22.1 27.075 25.25 10-14 26.515 26.424999999999997 22.795 24.265 15-19 25.755 25.314999999999998 24.805 24.125 20-24 25.056264066016503 26.626656664166042 24.36609152288072 23.950987746936732 25-29 25.456364091022753 25.006251562890725 24.701175293823454 24.836209052263065 30-34 25.046261565391347 26.62165541385346 24.081020255063766 24.251062765691422 35-39 25.4913728432108 25.46636659164791 24.711177794448613 24.33108277069267 40-44 25.541385346336583 25.831457864466117 23.74593648412103 24.88122030507627 45-49 25.496374093523382 25.7664416104026 24.046011502875718 24.691172793198298 50-54 25.36134033508377 26.38159539884971 24.191047761940485 24.066016504126033 55-59 26.5666416604151 25.34133533383346 24.111027756939237 23.980995248812203 60-64 25.831457864466117 25.716429107276817 24.151037759439863 24.301075268817204 65-69 25.81016203240648 25.31006201240248 24.7999599919984 24.079815963192637 70-74 25.586396599149786 25.041260315078766 25.221305326331585 24.151037759439863 75-79 25.78144536134033 25.101275318829707 24.841210302575647 24.276069017254315 80-84 25.081270317579396 26.586646661665412 23.995998999749936 24.336084021005252 85-89 26.62165541385346 25.63140785196299 24.15603900975244 23.59089772443111 90-94 25.481370342585645 25.541385346336583 24.91122780695174 24.066016504126033 95-99 26.116529132283073 25.586396599149786 24.221055263815956 24.07601900475119 100-104 26.6816704176044 26.461615403850963 23.490872718179546 23.365841460365093 105-109 25.621405351337835 26.611652913228305 24.381095273818453 23.385846461615404 110-114 27.6419104776194 26.556639159789945 23.705926481620406 22.09552388097024 115-119 27.321830457614404 26.76669167291823 23.680920230057513 22.230557639409852 120-124 27.081770442610654 26.76169042260565 23.090772693173292 23.065766441610403 125-129 27.09677419354839 26.701675418854716 23.465866466616657 22.735683920980247 130-134 28.09326061940261 26.006904487917147 23.665382498624105 22.234452394056138 135-139 27.742484117853035 26.241808813966284 24.16087239257666 21.85483467560402 140-144 28.1470367591898 25.90147536884221 24.031007751937985 21.92048012003001 145-149 29.148744623387017 25.797739321796538 23.462038611583473 21.59147744323297 150-151 28.085106382978726 26.99624530663329 23.46683354192741 21.451814768460576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 1.0 9 0.0 10 0.0 11 0.5 12 1.5 13 1.0 14 0.0 15 0.0 16 0.5 17 2.0 18 2.0 19 1.0 20 1.0 21 0.5 22 0.0 23 1.5 24 2.0 25 1.5 26 2.5 27 1.5 28 2.5 29 5.0 30 6.0 31 7.0 32 9.0 33 15.5 34 25.0 35 31.5 36 42.0 37 57.0 38 75.5 39 95.5 40 122.5 41 134.0 42 143.5 43 176.5 44 184.5 45 191.5 46 191.5 47 187.0 48 183.0 49 182.0 50 173.0 51 146.0 52 137.5 53 117.5 54 109.0 55 103.0 56 96.0 57 92.5 58 85.0 59 78.5 60 78.5 61 81.5 62 77.0 63 73.0 64 61.0 65 55.0 66 52.5 67 46.0 68 47.5 69 49.0 70 42.0 71 38.0 72 25.0 73 14.0 74 10.5 75 4.5 76 1.5 77 0.5 78 1.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.625 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.02 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.065 135-139 0.045 140-144 0.025 145-149 0.03 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.075 #Duplication Level Percentage of deduplicated Percentage of total 1 75.30350329517863 54.275 2 16.441207075962538 23.7 3 5.064169268123483 10.95 4 1.6996184530003469 4.9 5 0.9712105445716268 3.5000000000000004 6 0.2428026361429067 1.05 7 0.1387443635102324 0.7000000000000001 8 0.0 0.0 9 0.10405827263267431 0.675 >10 0.0346860908775581 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAA 10 0.25 No Hit GAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAA 9 0.22499999999999998 No Hit CAGATATGAACCAGTCAACGAACCATCTCTTGATAATTGCTTTGTCTCAG 9 0.22499999999999998 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 9 0.22499999999999998 No Hit GGGAAATTACATCTCTGGTGCTATCCTCTTCGAGGAGACCCTCTACCAAT 7 0.17500000000000002 No Hit AAGGAAATGAACGGAGGTTGACAGGGTTACATGAGACAGCTAGCATATCA 7 0.17500000000000002 No Hit CACGAGCGAGTTGTCCCGTTGGTCCCTGTACCGCGCCGCCATCGCCGAGT 7 0.17500000000000002 No Hit TGGCAGAAAAGCAGGTAGCGATTTAGAGGATAAGACTGTAACGTTTAGTA 7 0.17500000000000002 No Hit GGAAGCTCTTCATCTTTACCATCAGCGTATACTAGCCCTGATGTGCCTGG 6 0.15 No Hit GCCGGAACGAGCTGTTCCGGGAGCTCATCGCCGGGAACGCGGCCGTGTTC 6 0.15 No Hit CTGCTGGTCAGGAGCGATTCAGGACTATCACAACAGCATACTACAGGGGA 6 0.15 No Hit CCTCGGTGGCGCCGGCGGTGTAGGGTCCCTTATCATCCAGTTGGCGAAAC 6 0.15 No Hit AGAGCGTGGACAGTCATCGGCTGGCCACCAACAGGTTCGCGGACCTCACG 6 0.15 No Hit AGAGGGTTTTAGCAGAGAATATTCATGTGAGTTAAGTGTTAACCATGTTT 6 0.15 No Hit GGGAGGAGAGCGGCGGCTGCGACTGCACCACCTGCAAGTGCGGCACCGCC 6 0.15 No Hit GCGAAACAGCAGAGTCCAACTCCCTCTGTCCCTCCTAGTCAGTCACCGCA 5 0.125 No Hit GCAGCATCGGGCTAATTCTACTGGGGAAACCAAATAATATCATATCTAAC 5 0.125 No Hit ATCTATGTTTAAAAAGAGTCTAGATACATGTAATATTTCGGCAAGTAATT 5 0.125 No Hit CTCTGGGGCTCTGGTTCTGTGTGTAGAAAGAGAGCACAATACTAGGTGGG 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit CAGGAGTAGTGGAAATGTGAGAACTACTTCACAACCCAGTTCCTCCAAAA 5 0.125 No Hit CTATTATTGCAGGTTACAGAATGGCTGCTGAGTGTGCCAGAAATGCTTTG 5 0.125 No Hit TGGCAACATCTTGGCTACTGGGAGTCAAGACACAACATGTCGGCTGTGGG 5 0.125 No Hit ATTTCATCTCTCTCCCCCTTTCTTCCTTCCTCTCCGTGTGTTGAGAGAGG 5 0.125 No Hit ATTCGTTGGATCGAGTAGGCGATGCCGAATTTGGTGTCAATTTGTTGGAT 5 0.125 No Hit GCGGCACATACCAGGGGGTAGCGACTGTTTATTAAAAACACAGGACTCTG 5 0.125 No Hit GGGACAACTAGGTACTTCATTGTTAAGAGTTGCAATCGGGAGAACCTGGA 5 0.125 No Hit GAATAATACAGGATCATTCCCTCGAGGCTTGTCACTGATGCTTCGTTCGA 5 0.125 No Hit GTCTGACAGAAGCCGAGAGAAATTTTCTCATACAGTTTCTTCCAAGTGAG 5 0.125 No Hit CTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGA 5 0.125 No Hit CCGGAAGAGGATCGTCATCGAGATCGACCGACTCTTCTTCAAAATCATCA 5 0.125 No Hit GCTTTACATGAGCATGCAGTTGAGTTAATTCGGAAAGGACTAGCCTATGT 5 0.125 No Hit CTTAGAGTTCAACGTAACTAAACGAGAATCAAGTGGTTGCAAGGCTGCAT 5 0.125 No Hit GGAGAACGGCGGCTGCGGCTGCAGCACCTGCAAGTGCGGCACCAGCTGCG 5 0.125 No Hit CTGCAATTGCAAGCTTGTGTCAAAGAAGAGGGTAGCACCTGATCCTCTTG 5 0.125 No Hit GGTAATACTTGCATTTGGTCTAGTCCTTGGTGTCCTTTCTGGAATAACAT 5 0.125 No Hit GCCAGCGCATACGCTGATGAACTTGTCAAGACCGCGAAAACCATCGCATC 5 0.125 No Hit GATGAACTAAAGGCATCTTGCAATTTTGTGGAAGCTCGCAAAGATGTAAA 5 0.125 No Hit CTCCGGTGGTGATGGTGGCAGCTCAATCCTAACTGATGGTGACTCACCCG 5 0.125 No Hit GTTTAAATTAGGACTTCCAATGTGGGAGGATTGTCTGATGGCAGCTATTG 5 0.125 No Hit ATTGAACAGAACCGTGGGCTTTGTGCATCGACGTCAGAAGGAATGAGGAA 5 0.125 No Hit CACCTACAGACAAGGAAGGTGCTATCACACTTGTAGTTCGCATGCCTGAT 5 0.125 No Hit ACAAGATCGCCTCCAAGCTCCCAGAGGCTGACAAGAAGAAGATCGAGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.23750000000000002 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.36250000000000004 0.0 0.0 0.0 0.0 80-81 0.5125 0.0 0.0 0.0 0.0 82-83 0.7 0.0 0.0 0.0 0.0 84-85 0.875 0.0 0.0 0.0 0.0 86-87 1.0375 0.0 0.0 0.0 0.0 88-89 1.2625000000000002 0.0 0.0 0.0 0.0 90-91 1.5125000000000002 0.0 0.0 0.0 0.0 92-93 1.7375 0.0 0.0 0.0 0.0 94-95 2.0999999999999996 0.0 0.0 0.0 0.0 96-97 2.35 0.0 0.0 0.0 0.0 98-99 2.675 0.0 0.0 0.0 0.0 100-101 3.0125 0.0 0.0 0.0 0.0 102-103 3.4 0.0 0.0 0.0 0.0 104-105 3.7375 0.0 0.0 0.0 0.0 106-107 4.237500000000001 0.0 0.0 0.0 0.0 108-109 4.512499999999999 0.0 0.0 0.0 0.0 110-111 5.0625 0.0 0.0 0.0 0.0 112-113 5.487500000000001 0.0 0.0 0.0 0.0 114-115 6.012499999999999 0.0 0.0 0.0 0.0 116-117 6.6875 0.0 0.0 0.0 0.0 118-119 7.625 0.0 0.0 0.0 0.0 120-121 8.075 0.0 0.0 0.0 0.0 122-123 8.6625 0.0 0.0 0.0 0.0 124-125 9.649999999999999 0.0 0.0 0.0 0.0 126-127 10.2 0.0 0.0 0.0 0.0 128-129 11.125 0.0 0.0 0.0 0.0 130-131 11.9375 0.0 0.0 0.0 0.0 132-133 12.9375 0.0 0.0 0.0 0.0 134-135 13.6625 0.0 0.0 0.0 0.0 136-137 14.3875 0.0 0.0 0.0 0.0 138-139 14.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCCA 10 0.006830828 145.0 6 >>END_MODULE Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694251 spots for SRR13165354.sra Written 1694251 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra Read 1694232 spots for SRR13165354.sra Written 1694232 spots for SRR13165354.sra SRR ids: ['SRR13165354.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__rh6e1fj SRR13165354.sra spots: 33884659 blocks: [[1, 1694232], [1694233, 3388464], [3388465, 5082696], [5082697, 6776928], [6776929, 8471160], [8471161, 10165392], [10165393, 11859624], [11859625, 13553856], [13553857, 15248088], [15248089, 16942320], [16942321, 18636552], [18636553, 20330784], [20330785, 22025016], [22025017, 23719248], [23719249, 25413480], [25413481, 27107712], [27107713, 28801944], [28801945, 30496176], [30496177, 32190408], [32190409, 33884659]] SRR13165354 file size 11493789 SRR13165354 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165354 SRR13165354_1.fastq SRR13165354_2.fastq Input file: SRR13165354_1.fastq Paired file: SRR13165354_2.fastq trimmed: SRR13165354-trimmed-pair1.fastq, SRR13165354-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:03:39 2024 >> started Sat Dec 7 16:08:02 2024 >> done (263.832s) 33884659 read pairs processed; of these: 778 ( 0.00%) short read pairs filtered out after trimming by size control 24182 ( 0.07%) empty read pairs filtered out after trimming by size control 33859699 (99.93%) read pairs available; of these: 6181927 (18.26%) trimmed read pairs available after processing 27677772 (81.74%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 54 0.00% 19 44 0.00% 20 76 0.00% 21 63 0.00% 22 70 0.00% 23 87 0.00% 24 97 0.00% 25 111 0.00% 26 128 0.00% 27 121 0.00% 28 174 0.00% 29 168 0.00% 30 178 0.00% 31 203 0.00% 32 211 0.00% 33 174 0.00% 34 226 0.00% 35 190 0.00% 36 208 0.00% 37 277 0.00% 38 267 0.00% 39 306 0.00% 40 325 0.00% 41 305 0.00% 42 314 0.00% 43 349 0.00% 44 365 0.00% 45 398 0.00% 46 406 0.00% 47 490 0.00% 48 538 0.00% 49 584 0.00% 50 613 0.00% 51 736 0.00% 52 835 0.00% 53 908 0.00% 54 962 0.00% 55 1125 0.00% 56 1168 0.00% 57 1335 0.00% 58 1528 0.00% 59 1618 0.00% 60 2045 0.01% 61 2217 0.01% 62 2652 0.01% 63 2854 0.01% 64 3172 0.01% 65 3390 0.01% 66 3743 0.01% 67 4119 0.01% 68 4451 0.01% 69 5162 0.02% 70 5941 0.02% 71 6340 0.02% 72 7794 0.02% 73 8398 0.02% 74 9112 0.03% 75 10121 0.03% 76 11154 0.03% 77 12197 0.04% 78 13802 0.04% 79 15020 0.04% 80 16178 0.05% 81 17601 0.05% 82 19994 0.06% 83 22020 0.07% 84 24844 0.07% 85 26527 0.08% 86 28643 0.08% 87 30075 0.09% 88 31839 0.09% 89 33447 0.10% 90 36492 0.11% 91 38661 0.11% 92 40945 0.12% 93 43810 0.13% 94 47022 0.14% 95 49956 0.15% 96 53223 0.16% 97 55566 0.16% 98 56643 0.17% 99 59608 0.18% 100 62382 0.18% 101 64278 0.19% 102 66618 0.20% 103 68198 0.20% 104 70499 0.21% 105 72637 0.21% 106 75112 0.22% 107 76289 0.23% 108 79213 0.23% 109 82073 0.24% 110 81639 0.24% 111 84468 0.25% 112 87595 0.26% 113 87228 0.26% 114 90729 0.27% 115 92715 0.27% 116 94676 0.28% 117 96103 0.28% 118 97062 0.29% 119 99097 0.29% 120 101034 0.30% 121 101106 0.30% 122 103246 0.30% 123 104823 0.31% 124 106962 0.32% 125 109371 0.32% 126 108760 0.32% 127 111608 0.33% 128 110245 0.33% 129 112004 0.33% 130 112958 0.33% 131 114187 0.34% 132 116763 0.34% 133 116388 0.34% 134 116369 0.34% 135 119074 0.35% 136 118524 0.35% 137 118575 0.35% 138 118577 0.35% 139 121789 0.36% 140 122246 0.36% 141 122028 0.36% 142 125415 0.37% 143 124933 0.37% 144 126188 0.37% 145 129073 0.38% 146 131519 0.39% 147 140112 0.41% 148 132526 0.39% 149 137335 0.41% 150 134465 0.40% 151 27677772 81.74% 33859699 reads passed initial QC criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=2.72 fanout-score-rank=27 prefix-density=0.76 prefix-fanout=2.4 sequence=GCAGGTGCAGCTGGTGC criterion=fanout-score sequence-density=0.05 sequence-density-rank=35 fanout-score=125.72 fanout-score-rank=1 prefix-density=0.51 prefix-fanout=12.5 sequence=GGCGGCGGCCTCGCCGTCGCTGGTGTACTTCCCCAGCTGCGCCAGGGAGTTTGCCTTGGCGCGCAGCAGCAGTGCCTCCTGGGCCGCCGCCACGTTCTCCGGCCGTCCTCCCCACGTCTTCAGGCACGTGTTCTGCAGCGCCCTCGCGTATGAGAAGGACACGTGCCACGGGTTCGGCGACTGGTTCATCGCGTTCAGGTTCAGCGTTGCCTCCACCTCTGACTGCCCGCCCGACAGGAACATGATGCCGGGGACGGAAGGAGGGATCCTCCTCTGGAGGAGCTTGAGGGTGTAGCTGGCGACTTGCTCAGGGGTGGCCCGCTCCTTGCACTCGGCGCCGGGTGTCACCATGCTGGGCTTGAGGAGGATGCCCTCGAACAAGACGTTGTTCTGGGCCATGTAGTAGAAAGTCTCCGCCCACACCTTCTGCGCCACCTCGAAGGTCCTGTCGATGCCGTGCTCGCCGTCCAGCAGGATCTCCGGCTCCACAATCGGCACCAGACCGTTGTCC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.91 fanout-score-rank=24 prefix-density=0.39 prefix-fanout=2.5 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.09 sequence-density-rank=22 fanout-score=112.09 fanout-score-rank=1 prefix-density=0.64 prefix-fanout=16.3 sequence=GCCGCCGCCGCC SRR13165354 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:13:06 Started mapping on | Dec 07 16:13:07 Finished on | Dec 07 16:44:20 Mapping speed, Million of reads per hour | 65.08 Number of input reads | 33859699 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 31673789 Uniquely mapped reads % | 93.54% Average mapped length | 290.38 Number of splices: Total | 33481560 Number of splices: Annotated (sjdb) | 31257096 Number of splices: GT/AG | 33025741 Number of splices: GC/AG | 390117 Number of splices: AT/AC | 14075 Number of splices: Non-canonical | 51627 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.59 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 564814 % of reads mapped to multiple loci | 1.67% Number of reads mapped to too many loci | 103555 % of reads mapped to too many loci | 0.31% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.25% % of reads unmapped: other | 1.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1621456 1621456 1621456 N_multimapping 564814 564814 564814 N_noFeature 1468731 30816298 1716612 N_ambiguous 735017 5109 126275 UnstrandedReadsAssigned:29470041 PositiveStrandReadsAssigned:852382 NegativeStrandReadsAssigned:29830902 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165354 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165354-trimmed-pair1.fastq SRR13165354-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 33,859,699 reads, 30,286,390 reads pseudoaligned [quant] estimated average fragment length: 250.76 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,237 rounds 52973 SRR13165354.ke.tsv 35125 SRR13165354.se.tsv 88098 total ==> SRR13165354.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 687.156 0 0 PNS24247 1044 794.24 118.067 7.34718 PNS24249 1928 1678.24 76.3517 2.24859 PNS24246 1044 794.24 118.067 7.34718 PNS24248 1044 794.24 118.067 7.34718 PNS24244 1471 1221.24 178.448 7.22198 PNS24243 293 110.689 0 0 KQK14069 1603 1353.24 2326.43 84.9689 KQK14071 474 250.758 39.8356 7.85167 ==> SRR13165354.se.tsv <== BRADI_1g14170v3 2684 BRADI_1g53295v3 318 BRADI_1g59795v3 886 BRADI_1g07683v3 0 BRADI_1g00485v3 11 BRADI_1g20270v3 620 BRADI_1g74790v3 680 BRADI_1g09890v3 0 BRADI_1g77505v3 340 BRADI_1g48960v3 0 SRR13165354 completed mapping pipeline successfully