Starting /dee2/code/volunteer_pipeline.sh SRR13165355 current disk space = 1542178017280 free memory = 1606486196 SRR13165355 SRAfilesize 5345e41d90632c8f29d903867fda4d53 SRR13165355.sra SRR13165355.sra file validated SRR13165355 is paired end SRR13165355 is conventional basespace SRR13165355 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165355_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5095 37.0 37.0 37.0 37.0 37.0 2 36.098 37.0 37.0 37.0 37.0 37.0 3 36.49 37.0 37.0 37.0 37.0 37.0 4 36.43 37.0 37.0 37.0 37.0 37.0 5 36.605 37.0 37.0 37.0 37.0 37.0 6 36.5765 37.0 37.0 37.0 37.0 37.0 7 36.573 37.0 37.0 37.0 37.0 37.0 8 36.5645 37.0 37.0 37.0 37.0 37.0 9 36.533 37.0 37.0 37.0 37.0 37.0 10-14 36.5427 37.0 37.0 37.0 37.0 37.0 15-19 36.5347 37.0 37.0 37.0 37.0 37.0 20-24 36.486599999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4785 37.0 37.0 37.0 37.0 37.0 30-34 36.3751 37.0 37.0 37.0 37.0 37.0 35-39 36.4071 37.0 37.0 37.0 37.0 37.0 40-44 36.4106 37.0 37.0 37.0 37.0 37.0 45-49 36.3307 37.0 37.0 37.0 37.0 37.0 50-54 36.3486 37.0 37.0 37.0 37.0 37.0 55-59 36.28060000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.2774 37.0 37.0 37.0 37.0 37.0 65-69 36.1983 37.0 37.0 37.0 37.0 37.0 70-74 36.251400000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.2617 37.0 37.0 37.0 37.0 37.0 80-84 36.200599999999994 37.0 37.0 37.0 37.0 37.0 85-89 36.2727 37.0 37.0 37.0 37.0 37.0 90-94 36.1715 37.0 37.0 37.0 37.0 37.0 95-99 36.070800000000006 37.0 37.0 37.0 37.0 37.0 100-104 36.1424 37.0 37.0 37.0 37.0 37.0 105-109 36.169799999999995 37.0 37.0 37.0 37.0 37.0 110-114 36.1005 37.0 37.0 37.0 37.0 37.0 115-119 36.0912 37.0 37.0 37.0 37.0 37.0 120-124 35.9855 37.0 37.0 37.0 37.0 37.0 125-129 35.952999999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.918400000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.8278 37.0 37.0 37.0 37.0 37.0 140-144 35.634100000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.333600000000004 37.0 37.0 37.0 34.6 37.0 150-151 35.17075 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 0.0 22 0.0 23 4.0 24 1.0 25 5.0 26 4.0 27 13.0 28 17.0 29 16.0 30 26.0 31 43.0 32 47.0 33 93.0 34 145.0 35 334.0 36 2788.0 37 462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.60000000000001 8.275 5.025 31.1 2 23.0517848164907 9.778783308195074 33.43388637506284 33.73554550025138 3 21.425 13.700000000000001 25.4 39.475 4 28.000000000000004 21.125 22.0 28.875 5 28.875 25.674999999999997 23.175 22.275 6 26.724999999999998 28.975 20.825 23.474999999999998 7 21.15 22.85 35.175 20.825 8 21.025 22.875 29.525000000000002 26.575 9 21.0 19.625 32.35 27.025 10-14 24.975 25.215 24.775 25.035 15-19 25.185000000000002 23.595 24.355 26.865 20-24 24.83 23.880000000000003 24.87 26.419999999999998 25-29 25.290000000000003 23.635 24.32 26.755000000000003 30-34 26.47 23.799999999999997 23.885 25.845000000000002 35-39 24.94 23.695 24.884999999999998 26.479999999999997 40-44 24.645 24.52 23.974999999999998 26.86 45-49 24.69 23.705000000000002 24.4 27.205000000000002 50-54 25.424999999999997 23.07 24.03 27.474999999999998 55-59 25.5 22.81 24.98 26.71 60-64 24.925 23.445 24.39 27.24 65-69 24.779999999999998 23.705000000000002 24.32 27.195000000000004 70-74 25.705 23.599999999999998 23.72 26.974999999999998 75-79 25.374999999999996 23.62 23.735 27.27 80-84 25.275 23.72 24.169999999999998 26.834999999999997 85-89 25.75 24.4 23.21 26.640000000000004 90-94 25.53 23.915 23.195 27.36 95-99 25.46 23.485 23.56 27.495000000000005 100-104 25.69 23.325000000000003 23.425 27.560000000000002 105-109 26.040000000000003 22.78 23.865 27.315 110-114 26.31 23.955000000000002 22.875 26.86 115-119 26.43 23.315 23.355 26.900000000000002 120-124 25.540000000000003 24.525 22.82 27.115000000000002 125-129 25.745 23.98 22.425 27.85 130-134 26.009999999999998 23.805 22.91 27.275 135-139 26.224999999999998 23.965 22.205 27.605 140-144 25.515 23.845 22.96 27.68 145-149 26.029999999999998 23.990000000000002 23.54 26.44 150-151 27.1375 23.425 22.625 26.8125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 0.5 20 0.5 21 0.5 22 1.0 23 1.0 24 0.5 25 0.5 26 0.5 27 0.5 28 1.5 29 2.5 30 4.0 31 9.0 32 14.5 33 14.5 34 14.0 35 20.5 36 32.0 37 45.5 38 68.0 39 86.0 40 91.5 41 99.0 42 125.5 43 140.0 44 135.0 45 150.0 46 157.5 47 152.5 48 144.5 49 142.0 50 128.0 51 139.0 52 163.0 53 138.0 54 121.5 55 127.0 56 138.0 57 140.5 58 124.5 59 109.0 60 103.0 61 90.0 62 79.0 63 82.0 64 67.0 65 48.5 66 59.0 67 63.5 68 61.0 69 60.5 70 52.5 71 44.5 72 40.0 73 39.5 74 31.5 75 22.0 76 20.0 77 19.0 78 12.0 79 6.5 80 4.5 81 2.5 82 2.5 83 1.5 84 0.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5499999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.77499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66212826943747 50.7 2 17.87889645288427 24.95 3 5.446076675026872 11.4 4 2.4005732712289505 6.7 5 1.0032246506628448 3.5000000000000004 6 0.42995342171264783 1.7999999999999998 7 0.10748835542816196 0.525 8 0.03582945180938731 0.2 9 0.03582945180938731 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATACAATAACATCAACAGGAAGTCCTCGCTGAACCATATCATGGAACC 9 0.22499999999999998 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 8 0.2 No Hit CCCTCGGGACTGTCCTTTTGACTTCAACCTGAGCAGCTCATAAGTTGAGA 7 0.17500000000000002 No Hit GCGTCGCCGGCACGAGGGCCGTGCGATCCGTCGAGTTATCATGAATCATC 7 0.17500000000000002 No Hit CTAGTGGTGGTTGACGTAGCGGCATTGCTTCCTGATCTCTCCCTGGCTGC 7 0.17500000000000002 No Hit AGTAAACCTTGCAGTTGATCCTTCCCATTGATGATGATGAAGATGTTCTT 6 0.15 No Hit CCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT 6 0.15 No Hit GTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTC 6 0.15 No Hit GTGACGGAGAAAGTCTCGCCGGGGGCGTTGAGGTACTCCTCCTGGGAGAT 6 0.15 No Hit GCCGAGGGAGGTCGGATCCGGTGGCGGGACGGGTCATGGCCGACGGATCC 6 0.15 No Hit CCCTGGTCCGGTTGAGGCAACCTCCTCGACGCTTGAAGTGGCCCAGGACG 6 0.15 No Hit GACCTCCTCCAGCTCCTTGAGCACCTGTGTGGCGTCGGTGCACCCGAACA 6 0.15 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 6 0.15 No Hit CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA 6 0.15 No Hit GTCGTAGAAGAGGTCGTCGTCGATGGCGTCGGGGAGGAGGCCGGCGACGT 6 0.15 No Hit CCGGAAAGGAAAAACGCAAAGCAAAATGCCATGGTTGACGAAACCGGGCT 6 0.15 No Hit CCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAAT 6 0.15 No Hit GTCAACCCAACCTGAGCATTCATGTTAGCTCCATCCATATAGACCTGCCC 5 0.125 No Hit GCCGTCTTCTCGATAATCACGCGGATGTCGGGCGGGGGAAGGATGATGCC 5 0.125 No Hit GCGACATTTTCAAGAGAAACTCTAGCATCAGCATTAGCATATGAGTCCAT 5 0.125 No Hit GTGGAATTTCCTTTAGGCAGACGCCGGCTTGTCAAACTTGAGCGGCTTGA 5 0.125 No Hit GGTAGAAGAGGTGGCGGTGGACGGCCCAGTTGAGGGTGTCCTTGAGCGCG 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit CCCATGGCGATGAGATTCCCTTCTCATCCGACTCATTTCTTAGCCTCCGT 5 0.125 No Hit CATTTGGATTCCAATTGTTGAAGTCACCTACTAATGCTGCAGAATGTGCT 5 0.125 No Hit GTCTGGAGTGGGCAATAAACCAATTCCTGTGAAAAAAAAAATGGGTTCGC 5 0.125 No Hit GTCGTGGATCTCGTAGTCGCCGAGGGTGATGTGGTCCTTGTAGATGGTGT 5 0.125 No Hit TGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGT 5 0.125 No Hit ACTTTGTATTGGGTAGTCATCATCGGGCAGGTAAAAGTGTGTGGAAGCCA 5 0.125 No Hit GCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGC 5 0.125 No Hit CACGTAACTCTGTCTGCCACACCAAAATACCACTCACTAGACGCCAATGC 5 0.125 No Hit GTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCC 5 0.125 No Hit CAACTTAATCTGAAGCAAAGCAGGGCAAAGAGGTTCCAACAAAAAACTCC 5 0.125 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 5 0.125 No Hit GTCCTTGTCCTCCTTCCAGGAGACCTTGGGGCGGTAGGAGGTGACGAGGC 5 0.125 No Hit CTTTCCCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCC 5 0.125 No Hit GGCACGACAAGATTAGAGAAAGAAAGAGGCACACCACCATCCACGCTACT 5 0.125 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 5 0.125 No Hit GTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCTTTTAT 5 0.125 No Hit CCTCATTGTGCTTCATGATCCTTCCTTCTTGGCACTGGCGAAGGAGAGGA 5 0.125 No Hit GTCCGCCACCCGCTGCGCCGCCATCGCCGCGTCGGAGGTCGCCAGCGAGC 5 0.125 No Hit GTTGTTCATCAGCGATGTCAAAGCCAGCAAACCAATCTTGTTTGCTTAAT 5 0.125 No Hit ATCTTGCACAAGTTGTTCACTTTTATTCCCAGCAATTGCACACATCTCAA 5 0.125 No Hit GCCAAGTCGAGAAACCATGAAGTTACAGAAAACTGAAGTATACTGACAAC 5 0.125 No Hit AGCGGCTCGAAGACATCCGGCTTCTTGTCGTAGACCTCGGTCTTGAACTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0125 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.05 0.0 0.0 56-57 0.025 0.0 0.05 0.0 0.0 58-59 0.025 0.0 0.05 0.0 0.0 60-61 0.025 0.0 0.05 0.0 0.0 62-63 0.05 0.0 0.05 0.0 0.0 64-65 0.075 0.0 0.05 0.0 0.0 66-67 0.0875 0.0 0.05 0.0 0.0 68-69 0.125 0.0 0.05 0.0 0.0 70-71 0.125 0.0 0.05 0.0 0.0 72-73 0.1375 0.0 0.05 0.0 0.0 74-75 0.16249999999999998 0.0 0.05 0.0 0.0 76-77 0.1875 0.0 0.05 0.0 0.0 78-79 0.25 0.0 0.05 0.0 0.0 80-81 0.4125 0.0 0.05 0.0 0.0 82-83 0.6000000000000001 0.0 0.05 0.0 0.0 84-85 0.7625 0.0 0.05 0.0 0.0 86-87 1.0875 0.0 0.05 0.0 0.0 88-89 1.3 0.0 0.05 0.0 0.0 90-91 1.5125000000000002 0.0 0.05 0.0 0.0 92-93 1.7875 0.0 0.05 0.0 0.0 94-95 2.05 0.0 0.05 0.0 0.0 96-97 2.3625 0.0 0.05 0.0 0.0 98-99 2.85 0.0 0.05 0.0 0.0 100-101 3.3625 0.0 0.05 0.0 0.0 102-103 3.7375 0.0 0.05 0.0 0.0 104-105 4.05 0.0 0.05 0.0 0.0 106-107 4.4375 0.0 0.05 0.0 0.0 108-109 4.975 0.0 0.05 0.0 0.0 110-111 5.5375 0.0 0.05 0.0 0.0 112-113 6.2875 0.0 0.05 0.0 0.0 114-115 6.625 0.0 0.05 0.0 0.0 116-117 7.0125 0.0 0.05 0.0 0.0 118-119 7.5625 0.0 0.05 0.0 0.0 120-121 8.35 0.0 0.05 0.0 0.0 122-123 8.95 0.0 0.05 0.0 0.0 124-125 9.587499999999999 0.0 0.05 0.0 0.0 126-127 10.3125 0.0 0.05 0.0 0.0 128-129 11.0375 0.0 0.05 0.0 0.0 130-131 11.95 0.0 0.05 0.0 0.0 132-133 12.75 0.0 0.05 0.0 0.0 134-135 13.4375 0.0 0.05 0.0 0.0 136-137 14.125 0.0 0.05 0.0 0.0 138-139 14.7 0.0 0.05 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGGTC 10 0.006830828 145.0 145 CATTTGG 10 0.006830828 145.0 1 TTCTTTT 10 0.006830828 145.0 4 TGGATTC 10 0.006830828 145.0 5 TAACAAC 10 0.006830828 145.0 9 GATTCCA 10 0.006830828 145.0 7 >>END_MODULE SRR13165355 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165355_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2525 37.0 37.0 37.0 37.0 37.0 2 36.1675 37.0 37.0 37.0 37.0 37.0 3 36.202 37.0 37.0 37.0 37.0 37.0 4 36.233 37.0 37.0 37.0 37.0 37.0 5 36.332 37.0 37.0 37.0 37.0 37.0 6 36.2745 37.0 37.0 37.0 37.0 37.0 7 36.2645 37.0 37.0 37.0 37.0 37.0 8 36.193 37.0 37.0 37.0 37.0 37.0 9 36.2745 37.0 37.0 37.0 37.0 37.0 10-14 36.30839999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.2123 37.0 37.0 37.0 37.0 37.0 20-24 36.1913 37.0 37.0 37.0 37.0 37.0 25-29 36.1438 37.0 37.0 37.0 37.0 37.0 30-34 36.11800000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.072799999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.0572 37.0 37.0 37.0 37.0 37.0 45-49 35.98995000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.0262 37.0 37.0 37.0 37.0 37.0 55-59 35.998900000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.965700000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.97975 37.0 37.0 37.0 37.0 37.0 70-74 35.987 37.0 37.0 37.0 37.0 37.0 75-79 35.89465 37.0 37.0 37.0 37.0 37.0 80-84 35.939949999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.8757 37.0 37.0 37.0 37.0 37.0 90-94 35.84525 37.0 37.0 37.0 37.0 37.0 95-99 35.8529 37.0 37.0 37.0 37.0 37.0 100-104 35.7923 37.0 37.0 37.0 37.0 37.0 105-109 35.8124 37.0 37.0 37.0 37.0 37.0 110-114 35.6645 37.0 37.0 37.0 37.0 37.0 115-119 35.614 37.0 37.0 37.0 37.0 37.0 120-124 35.5407 37.0 37.0 37.0 37.0 37.0 125-129 35.38885 37.0 37.0 37.0 37.0 37.0 130-134 35.1841 37.0 37.0 37.0 34.6 37.0 135-139 35.053399999999996 37.0 37.0 37.0 32.2 37.0 140-144 34.906499999999994 37.0 37.0 37.0 25.0 37.0 145-149 34.616200000000006 37.0 37.0 37.0 25.0 37.0 150-151 34.208 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 3.0 14 7.0 15 4.0 16 1.0 17 1.0 18 2.0 19 1.0 20 5.0 21 6.0 22 7.0 23 10.0 24 6.0 25 4.0 26 7.0 27 9.0 28 16.0 29 17.0 30 29.0 31 45.0 32 63.0 33 133.0 34 214.0 35 488.0 36 2632.0 37 289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.94279979929754 18.991470145509282 6.874059207225288 26.191670847967885 2 31.574999999999996 21.7 24.325 22.400000000000002 3 24.65 24.2 26.0 25.15 4 29.15 28.050000000000004 19.475 23.325000000000003 5 28.675 31.7 18.625 21.0 6 25.474999999999998 35.85 16.625 22.05 7 23.575 18.825 31.15 26.450000000000003 8 24.5 22.6 22.725 30.175 9 25.775 21.525 25.95 26.75 10-14 26.935 25.995 21.12 25.95 15-19 27.26 24.834999999999997 22.58 25.324999999999996 20-24 26.96809042712814 24.257277183154947 22.88186455936781 25.892767830349108 25-29 27.208604302151073 24.33216608304152 22.526263131565784 25.93296648324162 30-34 26.735694277711087 24.499799919967987 22.268907563025213 26.49559823929572 35-39 27.093127938381517 24.59737921376413 23.036911073321996 25.27258177453236 40-44 26.87074829931973 25.200080032012806 22.128851540616246 25.80032012805122 45-49 27.159505827039464 23.83834342019707 23.5032261291452 25.49892462361827 50-54 27.81834550365109 24.037211163349003 22.506752025607682 25.637691307392217 55-59 27.908954477238616 24.102051025512754 21.98599299649825 26.00300150075038 60-64 27.42822846854056 23.49704911473442 23.001900570171053 26.072821846553968 65-69 26.42160540135034 24.68617154288572 22.67066766691673 26.22155538884721 70-74 27.26863431715858 23.816908454227114 22.78139069534767 26.133066533266636 75-79 27.85253364013806 23.760692311540193 22.8652893802211 25.521484668100648 80-84 27.061765441360343 23.460865216304075 22.8607151787947 26.616654163540886 85-89 26.67333666833417 24.482241120560282 22.291145572786395 26.55327663831916 90-94 27.51463012054219 24.618616515780523 22.222777972290302 25.643975391386988 95-99 27.978393518055416 23.60708212463739 23.136941082324697 25.277583274982497 100-104 28.479239619809903 24.092046023011505 22.791395697848923 24.637318659329665 105-109 29.754877438719358 23.25662831415708 22.311155577788895 24.677338669334667 110-114 28.82364709412824 24.46734020206062 22.071621486445935 24.63739121736521 115-119 29.47473736868434 24.242121060530263 21.85592796398199 24.427213606803402 120-124 29.05452726363182 24.01700850425213 22.10105052526263 24.827413706853427 125-129 29.278175178830473 24.43599619828923 21.84483017357811 24.440998449302185 130-134 29.610727509256478 24.35704993495447 21.610127088962276 24.42209546682678 135-139 30.398238943366017 23.724234540724435 21.407844706824093 24.46968180908545 140-144 31.319395818745623 23.036911073321996 22.511753526057817 23.131939581874562 145-149 32.09425655393236 23.193916349809886 21.27276365819492 23.43906343806284 150-151 32.232232232232235 23.71121121121121 22.334834834834837 21.72172172172172 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 0.5 22 1.0 23 2.0 24 1.0 25 0.5 26 0.5 27 0.0 28 0.5 29 2.5 30 4.0 31 6.5 32 11.5 33 13.0 34 15.5 35 25.5 36 35.0 37 50.0 38 65.5 39 76.5 40 99.0 41 116.0 42 123.5 43 128.0 44 124.5 45 127.5 46 141.0 47 146.0 48 139.5 49 134.0 50 117.5 51 126.0 52 140.0 53 122.5 54 114.0 55 133.0 56 144.0 57 112.5 58 102.0 59 102.5 60 104.0 61 105.0 62 94.5 63 97.0 64 96.0 65 77.5 66 68.0 67 70.0 68 68.5 69 64.5 70 60.0 71 45.5 72 41.0 73 45.0 74 33.0 75 26.5 76 19.5 77 16.5 78 10.0 79 5.5 80 4.5 81 2.5 82 1.5 83 2.0 84 3.5 85 1.5 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.5 97 1.0 98 2.0 99 2.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.04 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.025 70-74 0.05 75-79 0.045 80-84 0.025 85-89 0.05 90-94 0.034999999999999996 95-99 0.03 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.06999999999999999 135-139 0.06 140-144 0.03 145-149 0.06 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.625 #Duplication Level Percentage of deduplicated Percentage of total 1 73.14183123877918 50.925 2 17.127468581687612 23.849999999999998 3 5.780969479353681 12.075 4 2.2262118491921004 6.2 5 1.1490125673249552 4.0 6 0.2513464991023339 1.05 7 0.21543985637342908 1.05 8 0.0 0.0 9 0.0718132854578097 0.44999999999999996 >10 0.03590664272890485 0.4 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 16 0.4 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 9 0.22499999999999998 No Hit GGAAAACTTGTGGATCGTTTCTCATGTTCTAAGTTGATAAGTGACCTTTG 9 0.22499999999999998 No Hit GCTTCTTAGAGGGACTATCGCCGTTTAGGCGACGGAAGTTTGAGGCAATA 7 0.17500000000000002 No Hit GCCAAAGCCTCTTGCTAAGCGTACCACACTATACAGACGTGCGCGCGCAG 7 0.17500000000000002 No Hit GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA 7 0.17500000000000002 No Hit AGGACAAGCATACTAAGATGCCTCGTGGATTTGGATTTGTTACATTTTCT 7 0.17500000000000002 No Hit ACCTGATCCGCTCCAAGTGGGTTCCTTGCCTGGAGTTCAGCAAGGTCGGC 7 0.17500000000000002 No Hit GGATCGTCACGTTCAACTCCGACTGGGAGCTGCTGACGGAGAAGGAGGCG 7 0.17500000000000002 No Hit CGGTTATCAGCAGACACAAATGTTATTAATGTTGATGGAGGTTCTCCAAT 6 0.15 No Hit CGATCACGTTCAAGAACAACGCCGGGTTCCCCCACAACATCGTGTTCGAC 6 0.15 No Hit CCTTTCCGGTCAACCTCCATCTGGGGCCGAAACCCTAGAGACCACCGCCA 6 0.15 No Hit AACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTC 6 0.15 No Hit GAAATCATGAGCGCCATGGTCGCGTCGTCGCTGAACCCGGAGGCGCCGCT 6 0.15 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 6 0.15 No Hit ATTGACATGACCGCAAATGTTCTGGGATTCAAGCTCTCAATGCCCATCAT 6 0.15 No Hit GTTTGGCTGTGATAGTACAGCAGTTACAAATATACTTGGTCACCGCGACT 5 0.125 No Hit ATCCAACCTCTTCTGGAAGCACTTGCCATATTATTAGTTTGACAAAGGCG 5 0.125 No Hit GTTGAGGAAAGCTGCTGAAGCAAATAAGGACAACTTGTCTGCTCTGATGG 5 0.125 No Hit GCTCGTGACGCGAGGAGCTAGGGTTCGGGAGGGAAGGAGAAGCGGGCGAA 5 0.125 No Hit GCTCAGGAAGCACGGGTTGACCAACCACGCCGGCAGCGGCATAAGCAAGA 5 0.125 No Hit GCCAGATTCAAGGGCCTTTGTTGCAAGAAATCGGCAGGTACGGAGAAAGT 5 0.125 No Hit GCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTT 5 0.125 No Hit GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTA 5 0.125 No Hit CAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAGAAAATGCCTCG 5 0.125 No Hit GTTAAAGGAACCGGAGATATGGTGAATATGCATGTGTTTCCGGAGGATAA 5 0.125 No Hit CTCACTCCACCGTCCACTCCTCCGATGGCGTTCCTCCCGCTCCACCTCCC 5 0.125 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 5 0.125 No Hit AAATCCCCCAATCTCCTCCCGAACTCCCTTCTCTTCTCCCTGCCCGAAGC 5 0.125 No Hit AGTCTCTCCTGAAGCAGATCGAGTACCTGATCCGCTCCAAGTGGGTTCCT 5 0.125 No Hit CGCGATTTCCTTCACTTGTTCCTTGCAATAATTCCTGGACTGCTTTGTCC 5 0.125 No Hit GCCGGCGTCGAGCCGGAGCCTAAGCCTATGCCGCAGCCAGAGCCAAAGCC 5 0.125 No Hit TCCAAAAGCTAAGTCCGATACTGTTTCACCTCTGTGTCCAAAATCATGTA 5 0.125 No Hit CCACCAACGCAGAGGAGAGGAGAGAGAAGCTTCGAGAAGAAGAAGCGGTT 5 0.125 No Hit AGAAGGGTGGCAACAGGTTCATCAAGACCGCTGCCTACGGTCACTTTGGC 5 0.125 No Hit GTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCG 5 0.125 No Hit GTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTAC 5 0.125 No Hit CGGGAGCAGCCCAGCTTGAGAATCGGGCGGCCGTGCCGTCCGAATTGTAG 5 0.125 No Hit GGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATAGATC 5 0.125 No Hit GCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAA 5 0.125 No Hit CGGGTGGGTGCTCTTCAACATCCTCCAGCCGGCCCTCAACCAGCTCGAGA 5 0.125 No Hit GCCTGGCATGATCAGCATCAACCTTGACCTCAAGAAAGGCGGCAACAGGT 5 0.125 No Hit GAAGGATTTCGGAGCCATCTTGACTACCGATACAGCGAATACAAGAGAAT 5 0.125 No Hit ACGCGGGCTTTGCTCGCTGATCCGATGATTCATGATAACTCGACGGATCG 5 0.125 No Hit CCGCGGCCAACTGGTGCTACGCAACCGTCGCGCCCCGCGCTAAGAGCGTC 5 0.125 No Hit CTTTGATATCTGCTGATTTGCTTGATTTAGATGGTTTAGGAGGTGAGATT 5 0.125 No Hit GTTCTTGGGAGCGAGAAGTGGAAAGCTAGCGAGCTCTAGACGCAGATCAA 5 0.125 No Hit GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0125 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.16249999999999998 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.4125 0.0 0.0 0.0 0.0 82-83 0.6000000000000001 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 1.0875 0.0 0.0 0.0 0.0 88-89 1.3 0.0 0.0 0.0 0.0 90-91 1.5125000000000002 0.0 0.0 0.0 0.0 92-93 1.7875 0.0 0.0 0.0 0.0 94-95 2.0375 0.0 0.0 0.0 0.0 96-97 2.3875 0.0 0.0 0.0 0.0 98-99 2.8875 0.0 0.0 0.0 0.0 100-101 3.4125 0.0 0.0 0.0 0.0 102-103 3.7875 0.0 0.0 0.0 0.0 104-105 4.1 0.0 0.0 0.0 0.0 106-107 4.4875 0.0 0.0 0.0 0.0 108-109 5.0375 0.0 0.0 0.0 0.0 110-111 5.5875 0.0 0.0 0.0 0.0 112-113 6.325 0.0 0.0 0.0 0.0 114-115 6.675000000000001 0.0 0.0 0.0 0.0 116-117 7.075 0.0 0.0 0.0 0.0 118-119 7.6375 0.0 0.0 0.0 0.0 120-121 8.45 0.0 0.0 0.0 0.0 122-123 9.125 0.0 0.0 0.0 0.0 124-125 9.75 0.0 0.0 0.0 0.0 126-127 10.4875 0.0 0.0 0.0 0.0 128-129 11.2375 0.0 0.0 0.0 0.0 130-131 12.175 0.0 0.0 0.0 0.0 132-133 12.975000000000001 0.0 0.0 0.0 0.0 134-135 13.6625 0.0 0.0 0.0 0.0 136-137 14.3875 0.0 0.0 0.0 0.0 138-139 15.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGATTT 10 0.006830828 145.0 3 GGATTTC 10 0.006830828 145.0 4 GATTTCG 10 0.006830828 145.0 5 AAGGATT 10 0.006830828 145.0 2 ATTTCGG 10 0.006830828 145.0 6 GAAGGAT 10 0.006830828 145.0 1 >>END_MODULE Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500783 spots for SRR13165355.sra Written 1500783 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra Read 1500778 spots for SRR13165355.sra Written 1500778 spots for SRR13165355.sra SRR ids: ['SRR13165355.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1b36m132 SRR13165355.sra spots: 30015565 blocks: [[1, 1500778], [1500779, 3001556], [3001557, 4502334], [4502335, 6003112], [6003113, 7503890], [7503891, 9004668], [9004669, 10505446], [10505447, 12006224], [12006225, 13507002], [13507003, 15007780], [15007781, 16508558], [16508559, 18009336], [18009337, 19510114], [19510115, 21010892], [21010893, 22511670], [22511671, 24012448], [24012449, 25513226], [25513227, 27014004], [27014005, 28514782], [28514783, 30015565]] SRR13165355 file size 10178901 SRR13165355 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165355 SRR13165355_1.fastq SRR13165355_2.fastq Input file: SRR13165355_1.fastq Paired file: SRR13165355_2.fastq trimmed: SRR13165355-trimmed-pair1.fastq, SRR13165355-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:00:35 2024 >> started Sat Dec 7 16:01:14 2024 >> done (39.556s) 30015565 read pairs processed; of these: 829 ( 0.00%) short read pairs filtered out after trimming by size control 46626 ( 0.16%) empty read pairs filtered out after trimming by size control 29968110 (99.84%) read pairs available; of these: 5849196 (19.52%) trimmed read pairs available after processing 24118914 (80.48%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 55 0.00% 19 66 0.00% 20 70 0.00% 21 75 0.00% 22 62 0.00% 23 106 0.00% 24 128 0.00% 25 113 0.00% 26 152 0.00% 27 168 0.00% 28 153 0.00% 29 224 0.00% 30 225 0.00% 31 228 0.00% 32 232 0.00% 33 261 0.00% 34 275 0.00% 35 233 0.00% 36 307 0.00% 37 274 0.00% 38 329 0.00% 39 362 0.00% 40 389 0.00% 41 448 0.00% 42 412 0.00% 43 460 0.00% 44 503 0.00% 45 487 0.00% 46 550 0.00% 47 602 0.00% 48 661 0.00% 49 828 0.00% 50 870 0.00% 51 968 0.00% 52 1124 0.00% 53 1213 0.00% 54 1209 0.00% 55 1387 0.00% 56 1483 0.00% 57 1594 0.01% 58 1779 0.01% 59 1953 0.01% 60 2345 0.01% 61 2644 0.01% 62 2879 0.01% 63 3307 0.01% 64 3518 0.01% 65 3874 0.01% 66 4318 0.01% 67 4763 0.02% 68 5535 0.02% 69 6010 0.02% 70 6767 0.02% 71 7481 0.02% 72 8433 0.03% 73 9324 0.03% 74 10378 0.03% 75 11261 0.04% 76 12364 0.04% 77 13597 0.05% 78 14442 0.05% 79 16375 0.05% 80 17665 0.06% 81 19487 0.07% 82 21315 0.07% 83 23698 0.08% 84 25723 0.09% 85 27698 0.09% 86 30512 0.10% 87 31848 0.11% 88 33930 0.11% 89 35271 0.12% 90 37614 0.13% 91 40777 0.14% 92 43416 0.14% 93 45810 0.15% 94 47942 0.16% 95 51091 0.17% 96 53744 0.18% 97 55487 0.19% 98 57676 0.19% 99 59961 0.20% 100 61419 0.20% 101 64142 0.21% 102 64999 0.22% 103 67096 0.22% 104 69010 0.23% 105 69558 0.23% 106 72139 0.24% 107 74612 0.25% 108 75122 0.25% 109 79170 0.26% 110 79160 0.26% 111 80977 0.27% 112 84316 0.28% 113 84419 0.28% 114 87082 0.29% 115 89936 0.30% 116 90990 0.30% 117 90702 0.30% 118 90612 0.30% 119 91773 0.31% 120 97628 0.33% 121 96201 0.32% 122 96642 0.32% 123 100595 0.34% 124 101832 0.34% 125 102583 0.34% 126 103461 0.35% 127 103173 0.34% 128 102943 0.34% 129 105506 0.35% 130 103463 0.35% 131 106441 0.36% 132 107952 0.36% 133 107910 0.36% 134 107847 0.36% 135 109885 0.37% 136 111175 0.37% 137 108722 0.36% 138 108920 0.36% 139 110643 0.37% 140 109757 0.37% 141 110787 0.37% 142 112600 0.38% 143 113506 0.38% 144 115563 0.39% 145 115746 0.39% 146 114175 0.38% 147 116746 0.39% 148 115270 0.38% 149 115837 0.39% 150 115155 0.38% 151 24118914 80.48% 29968110 reads passed initial QC criterion=sequence-density sequence-density=0.71 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=28 prefix-density=0.70 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=23.03 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=3.4 sequence=AGGAGAGAGCACTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATAACTGGTACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACGA criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=3.44 fanout-score-rank=13 prefix-density=0.57 prefix-fanout=3.1 sequence=GAGTTCAGCAAGGTCGGCTTCGTCTTCCGCGAGCACAACAG criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=89.47 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=7.9 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGATGTCTGCTACCTTCTCGTCCACCGTCGGAGCTCCGGCTTCTACGCCAACCAGCTTCCTTGGGAAGAAGCTCAAGAAGCAGGTGACCTCGGCCGTGAACTACCATGGCAAGAGCACCAAGGCCAACAGATTCACAGTCATGGCCAAGGAGGTGGACGAGTCAAAGCAGACTGACCAGGACAGGTGGAAGGGCCTCGCCTACGATATCTCCGACGACCAGCAGGACATCACCAGGGGGAAGGGTATCGTCGACTCGCTCTTCCAGGCGCCCATGGGCGACGGTACCCACGTGGCCGTCCTCAGCTCCCAAGAGTACATCAGCCAGGGCCTAAGGAAGTACGACTTCGACAACA SRR13165355 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:03:18 Started mapping on | Dec 07 16:03:18 Finished on | Dec 07 16:06:30 Mapping speed, Million of reads per hour | 561.90 Number of input reads | 29968110 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 25200162 Uniquely mapped reads % | 84.09% Average mapped length | 289.64 Number of splices: Total | 22486172 Number of splices: Annotated (sjdb) | 21136791 Number of splices: GT/AG | 22180573 Number of splices: GC/AG | 255869 Number of splices: AT/AC | 9466 Number of splices: Non-canonical | 40264 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.85 Insertion rate per base | 0.01% Insertion average length | 2.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1421727 % of reads mapped to multiple loci | 4.74% Number of reads mapped to too many loci | 437692 % of reads mapped to too many loci | 1.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.99% % of reads unmapped: other | 6.72% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3346509 3346509 3346509 N_multimapping 1421727 1421727 1421727 N_noFeature 1595791 24508985 1842428 N_ambiguous 571650 3538 128335 UnstrandedReadsAssigned:23032721 PositiveStrandReadsAssigned:687639 NegativeStrandReadsAssigned:23229399 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165355 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165355-trimmed-pair1.fastq SRR13165355-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,968,110 reads, 23,936,497 reads pseudoaligned [quant] estimated average fragment length: 251.264 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,194 rounds 52973 SRR13165355.ke.tsv 35125 SRR13165355.se.tsv 88098 total ==> SRR13165355.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 686.33 0 0 PNS24247 1044 793.736 48.932 3.44171 PNS24249 1928 1677.74 243.572 8.10514 PNS24246 1044 793.736 48.932 3.44171 PNS24248 1044 793.736 48.932 3.44171 PNS24244 1471 1220.74 53.6324 2.4528 PNS24243 293 112.22 0 0 KQK14069 1603 1352.74 344.607 14.2222 KQK14071 474 250.786 2.30571 0.513285 ==> SRR13165355.se.tsv <== BRADI_1g14170v3 353 BRADI_1g53295v3 66 BRADI_1g59795v3 359 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 409 BRADI_1g74790v3 370 BRADI_1g09890v3 0 BRADI_1g77505v3 265 BRADI_1g48960v3 0 SRR13165355 completed mapping pipeline successfully