Starting /dee2/code/volunteer_pipeline.sh SRR13165356 current disk space = 1542153175040 free memory = 1606679072 SRR13165356 SRAfilesize b0577ffc772b87e03ac375840608bea6 SRR13165356.sra SRR13165356.sra file validated SRR13165356 is paired end SRR13165356 is conventional basespace SRR13165356 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165356_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6145 37.0 37.0 37.0 37.0 37.0 2 36.15925 37.0 37.0 37.0 37.0 37.0 3 36.53 37.0 37.0 37.0 37.0 37.0 4 36.5035 37.0 37.0 37.0 37.0 37.0 5 36.4985 37.0 37.0 37.0 37.0 37.0 6 36.5245 37.0 37.0 37.0 37.0 37.0 7 36.4845 37.0 37.0 37.0 37.0 37.0 8 36.595 37.0 37.0 37.0 37.0 37.0 9 36.5865 37.0 37.0 37.0 37.0 37.0 10-14 36.531800000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.4775 37.0 37.0 37.0 37.0 37.0 20-24 36.4927 37.0 37.0 37.0 37.0 37.0 25-29 36.3999 37.0 37.0 37.0 37.0 37.0 30-34 36.3719 37.0 37.0 37.0 37.0 37.0 35-39 36.393 37.0 37.0 37.0 37.0 37.0 40-44 36.3669 37.0 37.0 37.0 37.0 37.0 45-49 36.3196 37.0 37.0 37.0 37.0 37.0 50-54 36.29729999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.294 37.0 37.0 37.0 37.0 37.0 60-64 36.276700000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.1959 37.0 37.0 37.0 37.0 37.0 70-74 36.2505 37.0 37.0 37.0 37.0 37.0 75-79 36.233799999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.1554 37.0 37.0 37.0 37.0 37.0 85-89 36.160700000000006 37.0 37.0 37.0 37.0 37.0 90-94 36.142399999999995 37.0 37.0 37.0 37.0 37.0 95-99 36.038900000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.069399999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.0079 37.0 37.0 37.0 37.0 37.0 110-114 36.016299999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.993700000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.8994 37.0 37.0 37.0 37.0 37.0 125-129 35.85790000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.866499999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.8233 37.0 37.0 37.0 37.0 37.0 140-144 35.612199999999994 37.0 37.0 37.0 37.0 37.0 145-149 35.5144 37.0 37.0 37.0 37.0 37.0 150-151 35.21425 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 1.0 23 2.0 24 1.0 25 5.0 26 6.0 27 12.0 28 28.0 29 19.0 30 24.0 31 44.0 32 40.0 33 103.0 34 134.0 35 320.0 36 2844.0 37 415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.4 8.9 5.825 35.875 2 22.540880503144653 10.515723270440251 35.16981132075472 31.77358490566038 3 21.075 12.75 25.55 40.625 4 28.025 20.9 21.575 29.5 5 29.5 26.400000000000002 22.75 21.349999999999998 6 24.175 30.85 21.275 23.7 7 21.05 22.7 35.675000000000004 20.575 8 21.125 24.025 29.125 25.724999999999998 9 21.15 20.575 34.2 24.075 10-14 23.43 26.97 24.455 25.145 15-19 24.4 24.9 24.990000000000002 25.71 20-24 24.825 24.73 24.349999999999998 26.095000000000002 25-29 23.945 24.205 25.900000000000002 25.95 30-34 24.22 24.834999999999997 24.959999999999997 25.985000000000003 35-39 24.21 25.25 24.87 25.669999999999998 40-44 23.91 25.624999999999996 25.085 25.380000000000003 45-49 24.29 25.480000000000004 24.505 25.724999999999998 50-54 23.895 25.495 24.5 26.11 55-59 24.154999999999998 25.4 24.465 25.979999999999997 60-64 24.575 25.615 23.985 25.825 65-69 24.39 24.535 25.21 25.865 70-74 25.264999999999997 24.29 24.75 25.695 75-79 24.81 24.75 24.48 25.96 80-84 24.915000000000003 24.87 24.67 25.545 85-89 25.119999999999997 24.990000000000002 24.555 25.335 90-94 25.045 24.485 25.009999999999998 25.46 95-99 24.235 24.7 25.15 25.915 100-104 25.505 24.825 24.035 25.635 105-109 24.47 25.45 23.91 26.169999999999998 110-114 25.009999999999998 24.884999999999998 23.76 26.345000000000002 115-119 24.83 24.57 24.425 26.174999999999997 120-124 25.174999999999997 24.64 23.880000000000003 26.305 125-129 24.83 24.92 23.380000000000003 26.87 130-134 25.55 25.040000000000003 23.73 25.679999999999996 135-139 24.855 25.05 23.405 26.69 140-144 24.75 24.46 23.849999999999998 26.939999999999998 145-149 24.154999999999998 24.465 24.02 27.36 150-151 23.962500000000002 23.6625 23.799999999999997 28.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 1.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 1.0 26 1.0 27 2.5 28 4.0 29 4.0 30 4.5 31 11.5 32 15.0 33 14.5 34 26.5 35 41.0 36 45.0 37 46.5 38 64.5 39 89.5 40 101.0 41 112.0 42 142.0 43 165.0 44 182.5 45 196.0 46 198.5 47 188.5 48 178.0 49 176.5 50 166.5 51 157.5 52 140.5 53 129.5 54 123.5 55 102.0 56 95.0 57 79.5 58 73.0 59 81.5 60 82.5 61 85.5 62 70.5 63 58.0 64 55.0 65 63.0 66 61.0 67 55.0 68 49.0 69 33.5 70 40.0 71 42.5 72 28.0 73 22.5 74 23.0 75 22.0 76 19.5 77 13.5 78 6.5 79 3.0 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.625 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.55 #Duplication Level Percentage of deduplicated Percentage of total 1 72.92700212615166 51.449999999999996 2 17.46987951807229 24.65 3 6.2012756909992905 13.125 4 2.2324592487597448 6.3 5 0.7795889440113395 2.75 6 0.28348688873139616 1.2 7 0.10630758327427356 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCGAGGATTAGAGGGCGGCCGGCGTTGAGGGGAGCCGTGTAGCGGGAGG 7 0.17500000000000002 No Hit CTCGAATAAGACCAGTGGTCATTAGGTCCTTCAATTTTTATTAAAGCGAA 7 0.17500000000000002 No Hit GCCACGATCTCTCTGGCGAGGTTACTCGGACGGGGCAGGCATATCATGGT 7 0.17500000000000002 No Hit TTCCCCTCACTAGTTTGGCGTAGAACAATCTGCCGTATCAGTCTTGATAA 6 0.15 No Hit GAAAGAATTCGTGTTTCAATCCGACTTTGCTCCCCTAAAAGTATTCGTCG 6 0.15 No Hit GTAGAAATGAAAAAGAAATTTGGGAGGTACTACCTAAAAGAAAAGGAAAA 6 0.15 No Hit GGGTACTCCTTCTTCACCTCCTCCAGCTCCTTTAGCACCTGCGTGGCGTC 6 0.15 No Hit CCTCGAACGTGCAGAAGTTTCAGCTTCACGTTGTAGTGCAGAATGGTTCT 6 0.15 No Hit TGGCAGTGGGAGAAATCATGATGGGCATGGATATCTTGAATCCAAGAACA 6 0.15 No Hit CTGCACCATTTGATCCCAGTTCAGCATTTTGAGCTTCTGCTCCATTGCCA 6 0.15 No Hit GCCAGGACACATTCCTGACTGCACAGCAAAATTAAATTATGAAGGAATTC 6 0.15 No Hit GTCCATCTTAATACGGAGCTGTCCTTGGTTGTTTATGCCTTGCCGGATTC 5 0.125 No Hit CATTTGGCCAACCTTCTGTGGCAGTGCCAACAGCCAAGGAGACAAAAGCA 5 0.125 No Hit CTGCTGATGTAGGTCATGCAAGATGGAGACTCCAAGATCTTCAGTCTCCA 5 0.125 No Hit GGCGGCGGAGAGCGCGTTCAGCCCTCCCTGCGCGACACGGAAGTTGGCAT 5 0.125 No Hit CTCCAGTAAGATTCTGGAACTCAGGCCAGACGTCAATCTCAGATAATCCT 5 0.125 No Hit TCTACCCACGGTCCAGTTTCTAAGTCTTTCAGGACCACCTGATCTAATGT 5 0.125 No Hit TGCTAAGGTAGCCAAGTCCATCCTTCAGCACCCAATTTATCGCGGCTGCG 5 0.125 No Hit CTACAATCTCCCACTTTCCTAGCAGTTGATGCCACAGGTTTGGATATTCG 5 0.125 No Hit GCTCGTCACACACCACCTCCCAGAACTTGGCACCAATCTGGTTGCCACAC 5 0.125 No Hit GTGCAGCTGAACCAATGGCGAAACCCTTCCCAATAGCAGCAGTCGTGTTG 5 0.125 No Hit GCTACATGTGTCTTCCTAGCTTCGCTTCAGCTGGCTGCAGGTTAGCTCCT 5 0.125 No Hit GCCATATGTGAGCCTATAAGAGAAGGGGCCTCTTTCCCTTTCAGAGAATC 5 0.125 No Hit CGTACGGCTGCGTACAGCAACGACACCACACCACACGGCAACCATAACGC 5 0.125 No Hit CTACTGGCAAGAGAGTTGTTCTGCGCTTCTCTTGAGAGTAATTGTTGATT 5 0.125 No Hit CGTATGAATAAAGTTTCTAATAATACAATCTTTCAAGTGATCGAAGAAGG 5 0.125 No Hit GCCCGAGTCAATCCGTTCACGAGCAGTCTTATTAAACTTCTGAAGCACGA 5 0.125 No Hit GCCGACGTGCCGGTGATGTCGTTGTACTCGATCTTCTCCACCAGCGTCAC 5 0.125 No Hit CCCCATCAGATGAATTAATACTGCGCGATCTACACAACCACACACCACAT 5 0.125 No Hit CCCAGCAATTTGATTAACAGCCTATTGTGACATTCTCAATTCATATGAAT 5 0.125 No Hit GTCCTCACCCTCAAAAACTCCAGAACGAGATGAATGTGAAGTTCTAATCC 5 0.125 No Hit GTCACTGCAGAATGCGATTTTAGCTGTAGAGATGTACATAACTCCCATGA 5 0.125 No Hit GCCTTGTTCCAAATTCCAAAGAAGGAAAAAAGGAAGACAAACACACAGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.0625 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.21250000000000002 0.0 0.0 0.0 0.0 70-71 0.225 0.0 0.0 0.0 0.0 72-73 0.2375 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.325 0.0 0.0 0.0 0.0 80-81 0.3375 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.6 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 0.95 0.0 0.0 0.0 0.0 90-91 1.1124999999999998 0.0 0.0 0.0 0.0 92-93 1.35 0.0 0.0 0.0 0.0 94-95 1.625 0.0 0.0 0.0 0.0 96-97 1.9625000000000001 0.0 0.0 0.0 0.0 98-99 2.3125 0.0 0.0 0.0 0.0 100-101 2.6375 0.0 0.0 0.0 0.0 102-103 3.0875 0.0 0.0 0.0 0.0 104-105 3.625 0.0 0.0 0.0 0.0 106-107 4.1125 0.0 0.0 0.0 0.0 108-109 4.525 0.0 0.0 0.0 0.0 110-111 5.2 0.0 0.0 0.0 0.0 112-113 5.699999999999999 0.0 0.0 0.0 0.0 114-115 6.4875 0.0 0.0 0.0 0.0 116-117 7.199999999999999 0.0 0.0 0.0 0.0 118-119 7.9125 0.0 0.0 0.0 0.0 120-121 8.6875 0.0 0.0 0.0 0.0 122-123 9.4375 0.0 0.0 0.0 0.0 124-125 10.0875 0.0 0.0 0.0 0.0 126-127 10.9 0.0 0.0 0.0 0.0 128-129 11.625 0.0 0.0 0.0 0.0 130-131 12.3375 0.0 0.0 0.0 0.0 132-133 13.0875 0.0 0.0 0.0 0.0 134-135 13.912500000000001 0.0 0.0 0.0 0.0 136-137 14.625 0.0 0.0 0.0 0.0 138-139 15.399999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAAATC 10 0.006830828 145.0 3 AACGTGC 10 0.006830828 145.0 6 CCTCGAA 10 0.006830828 145.0 1 CGAACGT 10 0.006830828 145.0 4 ACGTGCA 10 0.006830828 145.0 7 >>END_MODULE SRR13165356 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165356_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.99275 37.0 37.0 37.0 37.0 37.0 2 36.2395 37.0 37.0 37.0 37.0 37.0 3 36.2055 37.0 37.0 37.0 37.0 37.0 4 36.254 37.0 37.0 37.0 37.0 37.0 5 36.298 37.0 37.0 37.0 37.0 37.0 6 36.2985 37.0 37.0 37.0 37.0 37.0 7 36.247 37.0 37.0 37.0 37.0 37.0 8 36.3225 37.0 37.0 37.0 37.0 37.0 9 36.32 37.0 37.0 37.0 37.0 37.0 10-14 36.290299999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.26649999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.22945 37.0 37.0 37.0 37.0 37.0 25-29 36.11305 37.0 37.0 37.0 37.0 37.0 30-34 36.08 37.0 37.0 37.0 37.0 37.0 35-39 36.13755 37.0 37.0 37.0 37.0 37.0 40-44 36.095150000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.153800000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.02995 37.0 37.0 37.0 37.0 37.0 55-59 36.01455 37.0 37.0 37.0 37.0 37.0 60-64 36.01995000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.979 37.0 37.0 37.0 37.0 37.0 70-74 35.91435 37.0 37.0 37.0 37.0 37.0 75-79 35.9441 37.0 37.0 37.0 37.0 37.0 80-84 35.9556 37.0 37.0 37.0 37.0 37.0 85-89 35.80545 37.0 37.0 37.0 37.0 37.0 90-94 35.8253 37.0 37.0 37.0 37.0 37.0 95-99 35.85325 37.0 37.0 37.0 37.0 37.0 100-104 35.819449999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.777249999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.759550000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.697050000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.60725 37.0 37.0 37.0 37.0 37.0 125-129 35.5957 37.0 37.0 37.0 37.0 37.0 130-134 35.42615 37.0 37.0 37.0 37.0 37.0 135-139 35.40715 37.0 37.0 37.0 37.0 37.0 140-144 35.20885 37.0 37.0 37.0 34.6 37.0 145-149 35.04835 37.0 37.0 37.0 29.8 37.0 150-151 34.56425 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 2.0 15 5.0 16 3.0 17 4.0 18 2.0 19 1.0 20 2.0 21 2.0 22 5.0 23 5.0 24 7.0 25 16.0 26 9.0 27 4.0 28 16.0 29 14.0 30 29.0 31 47.0 32 52.0 33 98.0 34 200.0 35 475.0 36 2699.0 37 300.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.22947526989706 19.73386894300778 8.385638965603816 29.65101682149134 2 30.075000000000003 22.45 26.85 20.625 3 22.125 22.975 30.049999999999997 24.85 4 27.175 29.075 19.75 24.0 5 27.925 31.924999999999997 18.7 21.45 6 23.724999999999998 32.975 20.75 22.55 7 24.2 18.475 32.574999999999996 24.75 8 22.325 21.45 24.4 31.825 9 22.675 23.200000000000003 25.775 28.349999999999998 10-14 26.305 25.590000000000003 22.575 25.53 15-19 25.515 25.53 23.265 25.69 20-24 26.023903585537834 24.623693554033103 23.603540531079663 25.748862329349404 25-29 25.914435826870154 25.063797848386287 23.947960970728047 25.073805354015512 30-34 26.91076430572229 24.204681872749102 23.694477791116448 25.19007603041217 35-39 26.498974846226936 24.958743811571736 23.39850977646647 25.143771565734863 40-44 26.326847081186532 24.24591065979691 23.81071482166975 25.616527437346807 45-49 26.272881864559366 24.942482744823447 23.662098629588876 25.122536761028307 50-54 25.953893083962594 24.528679301895284 23.683552532879933 25.833875081262192 55-59 26.554916187140353 24.203152364273205 24.113084813610207 25.12884663497623 60-64 25.54383157473621 24.298644796719508 25.448817322598387 24.708706305945892 65-69 26.76 25.019999999999996 23.165 25.055 70-74 26.79009256942707 24.08306229672254 23.722792094070552 25.404053039779832 75-79 26.028014007003502 24.14207103551776 23.991995997999 25.83791895947974 80-84 26.465 25.135 23.27 25.130000000000003 85-89 27.17266223044979 24.62600690448792 23.715415019762844 24.485915845299445 90-94 25.65513102620524 24.689937987597517 24.10482096419284 25.550110022004404 95-99 26.281314065703288 24.16120806040302 24.46122306115306 25.096254812740636 100-104 26.449837378033525 24.59844883662747 24.218163622717036 24.733550162621967 105-109 26.30973229922442 24.238178633975483 25.088816612459347 24.363272454340756 110-114 27.45411811771766 24.773716057408613 23.47852177826674 24.293644046606993 115-119 26.9975484064642 24.786110972131887 23.750437784559963 24.46590283684395 120-124 27.36552414310733 25.339004253189895 23.667750813109834 23.627720790592946 125-129 28.32699619771863 24.734840904542725 23.504102461476887 23.43406043626176 130-134 28.49637227920941 24.713535151363523 23.732799599699774 23.057292969727293 135-139 28.656492369276958 24.303227420565424 23.737803352514387 23.30247685764323 140-144 28.869330399559935 24.953743061459218 22.99844976746512 23.178476771515726 145-149 28.996747560670507 24.49337002752064 23.462596947710786 23.047285464098074 150-151 28.7215411558669 24.54340755566675 23.705278959219413 23.029772329246935 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.5 9 0.5 10 0.0 11 1.0 12 2.0 13 1.5 14 1.0 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 0.5 25 2.0 26 5.5 27 4.0 28 3.0 29 6.0 30 8.0 31 8.0 32 9.5 33 17.0 34 20.5 35 29.0 36 41.0 37 43.5 38 57.0 39 72.5 40 82.5 41 106.5 42 149.0 43 168.0 44 164.5 45 171.5 46 185.5 47 180.0 48 165.5 49 165.5 50 148.0 51 143.0 52 121.5 53 103.0 54 118.5 55 107.5 56 89.5 57 94.5 58 98.5 59 87.0 60 85.5 61 84.5 62 90.0 63 88.5 64 77.0 65 65.5 66 70.0 67 78.0 68 62.5 69 52.5 70 46.5 71 46.0 72 41.5 73 34.5 74 28.5 75 18.5 76 13.5 77 7.0 78 3.0 79 5.0 80 4.0 81 3.0 82 2.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.015 25-29 0.075 30-34 0.04 35-39 0.015 40-44 0.045 45-49 0.03 50-54 0.015 55-59 0.075 60-64 0.015 65-69 0.0 70-74 0.075 75-79 0.05 80-84 0.0 85-89 0.065 90-94 0.02 95-99 0.005 100-104 0.075 105-109 0.075 110-114 0.015 115-119 0.065 120-124 0.075 125-129 0.06 130-134 0.075 135-139 0.075 140-144 0.015 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.92500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 73.35213253436729 52.025 2 17.37751145576313 24.65 3 5.851251321818823 12.45 4 2.2559041240747266 6.4 5 0.7754670426506873 2.75 6 0.28198801550934083 1.2 7 0.10574550581600281 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTCTTGCAAATTGGCAATCACACAGCAGCAGCAAGATCAAGATCAAGA 7 0.17500000000000002 No Hit TCTTAGTCTTTCATTTCTTGTGTTTAACTCAAAACTACTCCTGTCTAGAA 7 0.17500000000000002 No Hit AGTTCCCAAACCAACACAAGCTAGCTAAAATGTGGCAATCCAAAGCCATC 7 0.17500000000000002 No Hit CTTGCCTCGAGTTCAGCAAGGTCGGTTTCGTCTTCCGTGAGCACGGCAAC 6 0.15 No Hit GCCTGTTTTGCGAAGGTCCGCCTCATGGACAAACTAAGATATCGACGAAT 6 0.15 No Hit GTTCCAGATATTATGGGCCGGGAAGGGAAGGTGCCCAACCCTGAGGAGGA 6 0.15 No Hit GAACAGTACTATTTCAGGGGCACTTGAAGCAAAAGTTGCTAGAACTGATC 6 0.15 No Hit AAAGAAAAGGGAGATCTCAATTTCATTGGCTGTACCACAAATGTGGCTCC 6 0.15 No Hit TCTTCTTCTTCTGCTTCTCCTGGTTTTGTTGGTTGCAGAGTACGTGGTGT 6 0.15 No Hit CATTGACTCCCATATTGACAATTCTGTTGCTGCGACCGCGCAAGCAAAAG 6 0.15 No Hit GGTAACACAACATTGATTAGTTTTCTGTAACTGTACCGTCCATATGTGTA 6 0.15 No Hit GTCCACACCCCCGCTCCCTCTCCCTCGCCGCCGACGGTTTCTCCTGCGCA 5 0.125 No Hit TTCTAGAGTGTGCCAAGAAAACTGACCAAACTGTGGTCTCAATTGCTCTT 5 0.125 No Hit AGTTCTTTATGATAAATATGAAAGGGTTTCCTTCATTAAATATGTGCTAG 5 0.125 No Hit CTGCAGCGCTACGTCAACTCGCCCAACGGGAAGAAAGTCATGGCCGCCTG 5 0.125 No Hit TGCAGCCCATGTTCTGCAAAACCGTTAAGGAGAACGGGAAAGTATCATTC 5 0.125 No Hit GCTACTAATGTCATCTTCGGTCTTGCTCTGGGATACAAGTCAGTTATCAT 5 0.125 No Hit CTTGAATCGGAGGCAGGAAATATATGGAGTAAACAAGTTTGCTGAGAGTG 5 0.125 No Hit GACCTTCCGTGTGGAGTTCTCCGTTTACAACCTAAAGGAGGATCTGGACG 5 0.125 No Hit CTGCACTCTCTCTCACCACCAACACAGAGGAATTAAGCAGAGGAAGATAC 5 0.125 No Hit CATTATTTGAGGATTTCTGAGGTTGCTGGAGCTGACCGCTCGTCAATAAT 5 0.125 No Hit CTGATACATGGACGTCTTCGAAAGACCGCCCAACATTACGTGGTGTGACC 5 0.125 No Hit AGGTTCTTGACTGGGAGCCCAAGGTCGTCCTGCGTGACGGCTTGGTGCTC 5 0.125 No Hit CAACAGCAAAGCTGTTCGTGAAGCGATGTGCTTCATGATGGACCCTTCTG 5 0.125 No Hit AGGCCTGCAAGGGGTCGCCAGCCAGATCAGCGGCGTGCTCTCCACTCAGG 5 0.125 No Hit CCTAGCTCTTCTCCGGCGCCCTCTTCCGTCCGTGATCGCTCTAAGAAAAA 5 0.125 No Hit GCAAGTTGTTGTCCGTGATATCGATCTTTTCTCATACTGTGAGTCGTGCT 5 0.125 No Hit CGTCGGAGATCAAATCCGGCATCGATGGCGCCGAGGCACTGATAAGGAAG 5 0.125 No Hit GCCACGGCATCAGTATCGGAGGCTTGGGCGAGAACGGCAGTCTGCACAAG 5 0.125 No Hit CAGAACCGCACACCGACAATTGTCATCGTCTGGGCTATTCTCCTTGCTTC 5 0.125 No Hit AAGAAGGGAAAGGGTAAGGTTGCAAACGGAGCAGCCCATCAAAGGGCAAA 5 0.125 No Hit TTTCTCTCCCAGTGTGATAAGTCGTGTGGGATGAAGGTTGCGAACCGGCT 5 0.125 No Hit CGGGAACCCTAAACCCAACTACCACTACTCGTCGTCGTCGCCGTGGTCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.0625 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.21250000000000002 0.0 0.0 0.0 0.0 70-71 0.225 0.0 0.0 0.0 0.0 72-73 0.2375 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.325 0.0 0.0 0.0 0.0 80-81 0.3375 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.6 0.0 0.0 0.0 0.0 86-87 0.8 0.0 0.0 0.0 0.0 88-89 0.95 0.0 0.0 0.0 0.0 90-91 1.1124999999999998 0.0 0.0 0.0 0.0 92-93 1.35 0.0 0.0 0.0 0.0 94-95 1.6375000000000002 0.0 0.0 0.0 0.0 96-97 1.9875 0.0 0.0 0.0 0.0 98-99 2.3375000000000004 0.0 0.0 0.0 0.0 100-101 2.6500000000000004 0.0 0.0 0.0 0.0 102-103 3.0875 0.0 0.0 0.0 0.0 104-105 3.6375 0.0 0.0 0.0 0.0 106-107 4.1375 0.0 0.0 0.0 0.0 108-109 4.5375 0.0 0.0 0.0 0.0 110-111 5.2 0.0 0.0 0.0 0.0 112-113 5.699999999999999 0.0 0.0 0.0 0.0 114-115 6.4875 0.0 0.0 0.0 0.0 116-117 7.225 0.0 0.0 0.0 0.0 118-119 7.95 0.0 0.0 0.0 0.0 120-121 8.7375 0.0 0.0 0.0 0.0 122-123 9.4875 0.0 0.0 0.0 0.0 124-125 10.1375 0.0 0.0 0.0 0.0 126-127 10.95 0.0 0.0 0.0 0.0 128-129 11.675 0.0 0.0 0.0 0.0 130-131 12.4375 0.0 0.0 0.0 0.0 132-133 13.2125 0.0 0.0 0.0 0.0 134-135 14.037500000000001 0.0 0.0 0.0 0.0 136-137 14.775 0.0 0.0 0.0 0.0 138-139 15.600000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGTAC 10 0.006830828 145.0 3 CAGTACT 10 0.006830828 145.0 4 AGTACTA 10 0.006830828 145.0 5 ATTGAGC 10 0.006830828 145.0 5 TACTATT 10 0.006830828 145.0 7 ACTATTT 10 0.006830828 145.0 8 CTATTTC 20 3.5877043E-4 108.75 9 >>END_MODULE Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456675 spots for SRR13165356.sra Written 1456675 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra Read 1456661 spots for SRR13165356.sra Written 1456661 spots for SRR13165356.sra SRR ids: ['SRR13165356.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_djtwmjj3 SRR13165356.sra spots: 29133234 blocks: [[1, 1456661], [1456662, 2913322], [2913323, 4369983], [4369984, 5826644], [5826645, 7283305], [7283306, 8739966], [8739967, 10196627], [10196628, 11653288], [11653289, 13109949], [13109950, 14566610], [14566611, 16023271], [16023272, 17479932], [17479933, 18936593], [18936594, 20393254], [20393255, 21849915], [21849916, 23306576], [23306577, 24763237], [24763238, 26219898], [26219899, 27676559], [27676560, 29133234]] SRR13165356 file size 9879047 SRR13165356 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165356 SRR13165356_1.fastq SRR13165356_2.fastq Input file: SRR13165356_1.fastq Paired file: SRR13165356_2.fastq trimmed: SRR13165356-trimmed-pair1.fastq, SRR13165356-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:02:49 2024 >> started Sat Dec 7 16:03:25 2024 >> done (35.913s) 29133234 read pairs processed; of these: 649 ( 0.00%) short read pairs filtered out after trimming by size control 9401 ( 0.03%) empty read pairs filtered out after trimming by size control 29123184 (99.97%) read pairs available; of these: 5603473 (19.24%) trimmed read pairs available after processing 23519711 (80.76%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 36 0.00% 19 34 0.00% 20 40 0.00% 21 42 0.00% 22 41 0.00% 23 46 0.00% 24 49 0.00% 25 59 0.00% 26 79 0.00% 27 69 0.00% 28 83 0.00% 29 92 0.00% 30 97 0.00% 31 110 0.00% 32 131 0.00% 33 138 0.00% 34 123 0.00% 35 138 0.00% 36 139 0.00% 37 148 0.00% 38 210 0.00% 39 202 0.00% 40 217 0.00% 41 218 0.00% 42 264 0.00% 43 282 0.00% 44 311 0.00% 45 317 0.00% 46 365 0.00% 47 375 0.00% 48 461 0.00% 49 525 0.00% 50 616 0.00% 51 604 0.00% 52 772 0.00% 53 871 0.00% 54 879 0.00% 55 872 0.00% 56 1012 0.00% 57 1144 0.00% 58 1368 0.00% 59 1555 0.01% 60 1736 0.01% 61 1908 0.01% 62 2184 0.01% 63 2299 0.01% 64 2632 0.01% 65 2890 0.01% 66 3340 0.01% 67 3567 0.01% 68 3870 0.01% 69 4397 0.02% 70 5186 0.02% 71 5452 0.02% 72 6394 0.02% 73 7164 0.02% 74 8045 0.03% 75 9088 0.03% 76 9817 0.03% 77 10664 0.04% 78 11718 0.04% 79 12947 0.04% 80 14210 0.05% 81 15872 0.05% 82 17239 0.06% 83 19247 0.07% 84 21445 0.07% 85 23048 0.08% 86 24786 0.09% 87 26284 0.09% 88 28184 0.10% 89 30349 0.10% 90 32163 0.11% 91 34443 0.12% 92 36605 0.13% 93 39320 0.14% 94 42321 0.15% 95 44692 0.15% 96 47098 0.16% 97 49453 0.17% 98 50883 0.17% 99 53404 0.18% 100 56077 0.19% 101 57278 0.20% 102 60003 0.21% 103 62288 0.21% 104 64950 0.22% 105 66915 0.23% 106 69180 0.24% 107 71444 0.25% 108 73396 0.25% 109 75817 0.26% 110 76264 0.26% 111 77663 0.27% 112 79572 0.27% 113 81835 0.28% 114 84358 0.29% 115 86419 0.30% 116 88096 0.30% 117 89705 0.31% 118 90536 0.31% 119 90943 0.31% 120 92785 0.32% 121 93835 0.32% 122 95563 0.33% 123 96439 0.33% 124 98126 0.34% 125 99437 0.34% 126 99808 0.34% 127 101012 0.35% 128 101809 0.35% 129 104418 0.36% 130 104492 0.36% 131 104144 0.36% 132 104978 0.36% 133 107668 0.37% 134 106760 0.37% 135 108759 0.37% 136 109037 0.37% 137 108778 0.37% 138 110283 0.38% 139 111842 0.38% 140 111602 0.38% 141 112411 0.39% 142 112923 0.39% 143 112986 0.39% 144 114429 0.39% 145 116292 0.40% 146 113588 0.39% 147 115730 0.40% 148 116021 0.40% 149 116638 0.40% 150 116663 0.40% 151 23519711 80.76% 29123184 reads passed initial QC criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=31 prefix-density=0.43 prefix-fanout=2.0 sequence=TAGGCGTCCGGGTACTCCTTCTTGACCTCCTCCAGCTCCTTGAGCACCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=86.63 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=7.4 sequence=GAGAAGAGGAATAATCATGTCATTACATCACTTGTATATATGCAACCTTTTAAGAGCAGCATTATTAATAGTACAGTTCGGTCCGACAGACGTCGACACAACCATTAGAAATTCAGGGTGATCAGCAGATCATGGAGAAAACGAAAGAAAAACAGAGGAAAAGCCAAGCAAAAAGTACATATGCAGATCGACCAAGGCAGAAGTGGAGATCGATCTCATCTCATAATCTCAAATGGATGGATGATTTGATCTACTTGGTGAACTCGATGGCCCACGCGTTGGCGTAGATGTATGTGGCCTTGACGCCGGTGAACCCGGCGCCCCGGGCGAGCTCCTCGAACTCCCTCTCGTACCTCTCCTTGCCCCCCGGGTTGTGGGCGAGCATGATCATGTCGACGTGGAACACCCCTTGTGCCTTTGGGGTGGCCTCCGGGTTCACCGGCAGGATGCACTCGACGATGACCACTTTCCCGTGTGCGGGGAGCGCGTCGTAGCAGTTCTTGAGCAGCGT criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=4.12 fanout-score-rank=15 prefix-density=0.47 prefix-fanout=3.6 sequence=GAGTTCAGCAAGGTCGGCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=128.08 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=10.9 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAG SRR13165356 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:04:09 Started mapping on | Dec 07 16:04:09 Finished on | Dec 07 16:06:30 Mapping speed, Million of reads per hour | 743.57 Number of input reads | 29123184 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 28098700 Uniquely mapped reads % | 96.48% Average mapped length | 289.85 Number of splices: Total | 25955748 Number of splices: Annotated (sjdb) | 24456513 Number of splices: GT/AG | 25630637 Number of splices: GC/AG | 270599 Number of splices: AT/AC | 12766 Number of splices: Non-canonical | 41746 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 3.08 Insertion rate per base | 0.01% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 216610 % of reads mapped to multiple loci | 0.74% Number of reads mapped to too many loci | 27878 % of reads mapped to too many loci | 0.10% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.26% % of reads unmapped: other | 0.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 808230 808230 808230 N_multimapping 216610 216610 216610 N_noFeature 926843 27374923 1176566 N_ambiguous 541745 3405 68553 UnstrandedReadsAssigned:26630112 PositiveStrandReadsAssigned:720372 NegativeStrandReadsAssigned:26853581 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165356 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165356-trimmed-pair1.fastq SRR13165356-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,123,184 reads, 27,047,706 reads pseudoaligned [quant] estimated average fragment length: 240.95 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,207 rounds 52973 SRR13165356.ke.tsv 35125 SRR13165356.se.tsv 88098 total ==> SRR13165356.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 696.552 0 0 PNS24247 1044 804.05 31.1942 2.24616 PNS24249 1928 1688.05 145.492 4.99004 PNS24246 1044 804.05 31.1942 2.24616 PNS24248 1044 804.05 31.1942 2.24616 PNS24244 1471 1231.05 53.9251 2.53609 PNS24243 293 111.014 0 0 KQK14069 1603 1363.05 0 0 KQK14071 474 254.228 0 0 ==> SRR13165356.se.tsv <== BRADI_1g14170v3 0 BRADI_1g53295v3 51 BRADI_1g59795v3 381 BRADI_1g07683v3 0 BRADI_1g00485v3 28 BRADI_1g20270v3 949 BRADI_1g74790v3 424 BRADI_1g09890v3 4 BRADI_1g77505v3 305 BRADI_1g48960v3 0 SRR13165356 completed mapping pipeline successfully