Starting /dee2/code/volunteer_pipeline.sh SRR13165357 current disk space = 1542148620288 free memory = 1602317860 SRR13165357 SRAfilesize a5cdac19b7a074f03b25920f091c667b SRR13165357.sra SRR13165357.sra file validated SRR13165357 is paired end SRR13165357 is conventional basespace SRR13165357 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165357_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6115 37.0 37.0 37.0 37.0 37.0 2 36.17175 37.0 37.0 37.0 37.0 37.0 3 36.5755 37.0 37.0 37.0 37.0 37.0 4 36.563 37.0 37.0 37.0 37.0 37.0 5 36.597 37.0 37.0 37.0 37.0 37.0 6 36.526 37.0 37.0 37.0 37.0 37.0 7 36.466 37.0 37.0 37.0 37.0 37.0 8 36.588 37.0 37.0 37.0 37.0 37.0 9 36.512 37.0 37.0 37.0 37.0 37.0 10-14 36.547900000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.557 37.0 37.0 37.0 37.0 37.0 20-24 36.518899999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.449200000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.4183 37.0 37.0 37.0 37.0 37.0 35-39 36.360600000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.3177 37.0 37.0 37.0 37.0 37.0 45-49 35.9211 37.0 37.0 37.0 37.0 37.0 50-54 36.1717 37.0 37.0 37.0 37.0 37.0 55-59 35.7243 37.0 37.0 37.0 37.0 37.0 60-64 35.7019 37.0 37.0 37.0 37.0 37.0 65-69 35.4513 37.0 37.0 37.0 37.0 37.0 70-74 35.761799999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.2428 37.0 37.0 37.0 37.0 37.0 80-84 36.1253 37.0 37.0 37.0 37.0 37.0 85-89 36.1652 37.0 37.0 37.0 37.0 37.0 90-94 36.15069999999999 37.0 37.0 37.0 37.0 37.0 95-99 36.0715 37.0 37.0 37.0 37.0 37.0 100-104 36.124 37.0 37.0 37.0 37.0 37.0 105-109 36.128699999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.997 37.0 37.0 37.0 37.0 37.0 115-119 36.093599999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.9308 37.0 37.0 37.0 37.0 37.0 125-129 35.8537 37.0 37.0 37.0 37.0 37.0 130-134 35.8327 37.0 37.0 37.0 37.0 37.0 135-139 35.719 37.0 37.0 37.0 37.0 37.0 140-144 35.612 37.0 37.0 37.0 37.0 37.0 145-149 35.258799999999994 37.0 37.0 37.0 32.2 37.0 150-151 35.179500000000004 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 3.0 22 3.0 23 3.0 24 6.0 25 5.0 26 5.0 27 15.0 28 16.0 29 16.0 30 33.0 31 39.0 32 52.0 33 93.0 34 269.0 35 348.0 36 2708.0 37 385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.37499999999999 12.475 5.5 28.65 2 22.37339380196523 14.36130007558579 29.428067523305618 33.83723859914336 3 17.849999999999998 14.025000000000002 28.275 39.85 4 22.825 19.175 21.475 36.525 5 29.875 24.55 21.725 23.849999999999998 6 29.2 26.950000000000003 19.475 24.375 7 17.775 29.049999999999997 35.199999999999996 17.974999999999998 8 19.3 28.799999999999997 28.775000000000002 23.125 9 24.224999999999998 21.9 28.925 24.95 10-14 22.11 28.325 22.935 26.63 15-19 22.900000000000002 25.185000000000002 24.765 27.150000000000002 20-24 22.905 26.39 24.605 26.1 25-29 23.335 25.16 23.94 27.565 30-34 21.92 25.874999999999996 24.445 27.76 35-39 23.22 25.900000000000002 24.69 26.19 40-44 23.115 25.795 24.445 26.645000000000003 45-49 23.335 25.025 24.93 26.71 50-54 23.555 24.165 25.035 27.245 55-59 22.435 24.575 26.31 26.68 60-64 23.315 23.580000000000002 25.314999999999998 27.79 65-69 23.84 26.974999999999998 23.14 26.045 70-74 26.985 23.145 23.585 26.284999999999997 75-79 27.189999999999998 23.84 22.595000000000002 26.375 80-84 27.24 23.885 23.3 25.575 85-89 27.07 23.965 22.795 26.169999999999998 90-94 27.685 24.305 22.89 25.119999999999997 95-99 27.215 23.825 22.86 26.1 100-104 26.700000000000003 23.235 23.76 26.305 105-109 27.805000000000003 23.830000000000002 22.285 26.08 110-114 27.52 23.5 22.735 26.245 115-119 28.194999999999997 22.535 22.73 26.540000000000003 120-124 27.779999999999998 23.799999999999997 22.025 26.395000000000003 125-129 27.450000000000003 24.265 21.89 26.395000000000003 130-134 27.389999999999997 23.150000000000002 22.365 27.095000000000002 135-139 28.21 23.285 22.37 26.135 140-144 27.98 23.064999999999998 22.189999999999998 26.765 145-149 27.925 22.13 23.155 26.790000000000003 150-151 27.975 22.85 22.45 26.724999999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 1.5 2 1.0 3 0.5 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 1.5 10 2.0 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.5 23 1.0 24 1.0 25 0.5 26 0.0 27 0.5 28 3.5 29 4.5 30 3.0 31 6.0 32 9.5 33 7.0 34 18.0 35 32.0 36 32.5 37 42.5 38 53.5 39 69.5 40 93.0 41 111.0 42 132.5 43 151.5 44 169.5 45 164.5 46 156.5 47 174.5 48 180.5 49 196.5 50 191.5 51 165.0 52 155.5 53 158.0 54 135.5 55 122.0 56 121.0 57 100.0 58 98.5 59 83.5 60 70.5 61 59.0 62 49.0 63 45.5 64 49.0 65 92.5 66 116.0 67 90.0 68 56.5 69 32.5 70 23.5 71 24.0 72 30.0 73 25.5 74 14.5 75 13.5 76 13.5 77 13.0 78 11.0 79 4.5 80 3.5 81 3.0 82 1.5 83 1.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.775 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.75 #Duplication Level Percentage of deduplicated Percentage of total 1 70.49429657794677 46.35 2 18.935361216730037 24.9 3 6.692015209125475 13.200000000000001 4 2.2053231939163496 5.800000000000001 5 1.1787072243346008 3.875 6 0.22813688212927757 0.8999999999999999 7 0.1520912547528517 0.7000000000000001 8 0.0 0.0 9 0.03802281368821293 0.22499999999999998 >10 0.0 0.0 >50 0.07604562737642585 4.05 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCTCGTAT 97 2.4250000000000003 TruSeq Adapter, Index 8 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCGCGTAT 65 1.625 TruSeq Adapter, Index 8 (97% over 39bp) GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 9 0.22499999999999998 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 7 0.17500000000000002 No Hit CCCCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTA 7 0.17500000000000002 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 7 0.17500000000000002 No Hit CTGGTGAGCATCTCGTTCAGCTCGGCGTAGAACACGCCATCGCTCACAAA 7 0.17500000000000002 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 6 0.15 No Hit GCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGC 6 0.15 No Hit CCCGCCGAAGGGGATCGCCGCGTCGAAGATGTCGAAGCAGTTCACCCAGA 6 0.15 No Hit CCGTGGATCAGTAAATTGCCTGGAGGGGTCTTCGTCTCAAACTCCAGAGT 6 0.15 No Hit GGCAGCTTAATTAGACGTGCGTGCAGGATTTCTTGAGGGAGACGGGCTTG 6 0.15 No Hit AGGGTGATCAGCAGATCATGGAGAAAACGAAAGAAAAACAGAGGAAAAGC 6 0.15 No Hit GGCAGCAACAGACTGCATAACCATCTAGTTCACACCATATAAAACGATGG 5 0.125 No Hit GCAGGAATGGTTCACCAAGGCTAGAAATACATCAAGAACGAATCTTTGCG 5 0.125 No Hit GCCTGGGAGGTACCGGTAATCATGTTCTTGATGAAGTCACGGTGTCCAGG 5 0.125 No Hit ACTGACCATAGTTCACAACGAGCAATCCAAAATACCAGAGCACACGTCCA 5 0.125 No Hit GTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATC 5 0.125 No Hit AGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGC 5 0.125 No Hit GTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCG 5 0.125 No Hit CTCCCGATTCCATGGCGCGGCTCACCGGAGCAGCCGCGCCGTCCTACCTA 5 0.125 No Hit GTTCAAAGACTCGATGGTTCGCGGGATTCTGCAATTCACACCAGGTATCG 5 0.125 No Hit GTTCTGACTATCAATTTAGTAAACAGAAAAGAGTAGATAAAATAAAATCA 5 0.125 No Hit GGTGCTTCTTGCAAAGTGCCAAAGTTTATTCCTTAAGAGGTCCCTTCTGG 5 0.125 No Hit ACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAG 5 0.125 No Hit GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 5 0.125 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 5 0.125 No Hit GGTTGATGTTAATTTTCCATGGTTGATGATTTGTTCCAAGTAACAGCATA 5 0.125 No Hit GGCAGGAAGAAATATATGACCAATTAGATCAATTGCTGCAGACCGTGACT 5 0.125 No Hit CCCATTAAACCGAACAGGTGTATGAAACATAGGGTGCATGCTGATAACTG 5 0.125 No Hit AGCTTGGATTCATTAATCATCGCTGAGCGCGAGCAGCAGAACAAGGGAAC 5 0.125 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 5 0.125 No Hit CTGGTCATACTCACATTCTCTTCTCTCCAAGTACTGCATTGCCTCTCGCT 5 0.125 No Hit GTACCCTGTGGCCAATTACATTACAGTGAGCTAGCGAACGCGACTCGGCG 5 0.125 No Hit GGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCA 5 0.125 No Hit GTCTAATCTAAGTTTTCCATCATGGCGAAATATCCGGTGAAAAAGAAAAC 5 0.125 No Hit AGCATGTCAGGATCATTCCATCCACCAGGTCCGGCGTAGGAGGCCCATCT 5 0.125 No Hit GTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTGGGGAAGGGAGCTTCGAG 5 0.125 No Hit GTCCCGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCAGGGTCG 5 0.125 No Hit CGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCC 5 0.125 No Hit GTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTA 5 0.125 No Hit CTTAGATCACAAACTCGCAGTCCGTTTCGAGTGACCTTGCTGGAATAACA 5 0.125 No Hit GGGGAAGGGAGCTTCGAGGCGGCCGGACGCGGCTCGTCGGCCGGAACGGC 5 0.125 No Hit GGGCAGGCGCCACACCAACAAAAGAAGCGACAGGATCTGAAAAGAACGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.037500000000000006 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.0625 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.175 0.0 0.0 0.0 0.0 64-65 0.2375 0.0 0.0 0.0 0.0 66-67 0.3875 0.0 0.0 0.0 0.0 68-69 0.42500000000000004 0.0 0.0 0.0 0.0 70-71 0.4625 0.0 0.0 0.0 0.0 72-73 0.4875 0.0 0.0 0.0 0.0 74-75 0.5875 0.0 0.0 0.0 0.0 76-77 1.0125000000000002 0.0 0.0 0.0 0.0 78-79 1.2374999999999998 0.0 0.0 0.0 0.0 80-81 1.35 0.0 0.0 0.0 0.0 82-83 1.625 0.0 0.0 0.0 0.0 84-85 1.875 0.0 0.0 0.0 0.0 86-87 2.1500000000000004 0.0 0.0 0.0 0.0 88-89 2.8 0.0 0.0 0.0 0.0 90-91 3.1625 0.0 0.0 0.0 0.0 92-93 3.45 0.0 0.0 0.0 0.0 94-95 4.0 0.0 0.0 0.0 0.0 96-97 4.699999999999999 0.0 0.0 0.0 0.0 98-99 5.300000000000001 0.0 0.0 0.0 0.0 100-101 6.1125 0.0 0.0 0.0 0.0 102-103 6.825 0.0 0.0 0.0 0.0 104-105 7.5875 0.0 0.0 0.0 0.0 106-107 8.2 0.0 0.0 0.0 0.0 108-109 8.925 0.0 0.0 0.0 0.0 110-111 9.649999999999999 0.0 0.0 0.0 0.0 112-113 10.337499999999999 0.0 0.0 0.0 0.0 114-115 10.9875 0.0 0.0 0.0 0.0 116-117 11.7 0.0 0.0 0.0 0.0 118-119 12.75 0.0 0.0 0.0 0.0 120-121 13.6875 0.0 0.0 0.0 0.0 122-123 14.5125 0.0 0.0 0.0 0.0 124-125 15.274999999999999 0.0 0.0 0.0 0.0 126-127 16.0625 0.0 0.0 0.0 0.0 128-129 16.8625 0.0 0.0 0.0 0.0 130-131 17.9375 0.0 0.0 0.0 0.0 132-133 18.5625 0.0 0.0 0.0 0.0 134-135 19.5625 0.0 0.0 0.0 0.0 136-137 20.475 0.0 0.0 0.0 0.0 138-139 21.4625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13165357 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165357_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0685 37.0 37.0 37.0 37.0 37.0 2 36.2385 37.0 37.0 37.0 37.0 37.0 3 36.1025 37.0 37.0 37.0 37.0 37.0 4 36.1185 37.0 37.0 37.0 37.0 37.0 5 36.225 37.0 37.0 37.0 37.0 37.0 6 36.175 37.0 37.0 37.0 37.0 37.0 7 36.141 37.0 37.0 37.0 37.0 37.0 8 35.853 37.0 37.0 37.0 37.0 37.0 9 35.858 37.0 37.0 37.0 37.0 37.0 10-14 35.7136 37.0 37.0 37.0 37.0 37.0 15-19 35.6194 37.0 37.0 37.0 37.0 37.0 20-24 35.56915 37.0 37.0 37.0 37.0 37.0 25-29 35.183749999999996 37.0 37.0 37.0 37.0 37.0 30-34 35.106399999999994 37.0 37.0 37.0 37.0 37.0 35-39 34.97255 37.0 37.0 37.0 34.6 37.0 40-44 34.99635000000001 37.0 37.0 37.0 37.0 37.0 45-49 34.9302 37.0 37.0 37.0 34.6 37.0 50-54 34.977050000000006 37.0 37.0 37.0 37.0 37.0 55-59 35.072649999999996 37.0 37.0 37.0 34.6 37.0 60-64 35.27785000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.122150000000005 37.0 37.0 37.0 37.0 37.0 70-74 34.881150000000005 37.0 37.0 37.0 27.4 37.0 75-79 34.81544999999999 37.0 37.0 37.0 27.4 37.0 80-84 34.98395000000001 37.0 37.0 37.0 32.2 37.0 85-89 35.2271 37.0 37.0 37.0 34.6 37.0 90-94 35.4529 37.0 37.0 37.0 37.0 37.0 95-99 35.564949999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.57845 37.0 37.0 37.0 37.0 37.0 105-109 35.57695 37.0 37.0 37.0 37.0 37.0 110-114 35.471349999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.367850000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.301249999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.17685 37.0 37.0 37.0 37.0 37.0 130-134 34.97345 37.0 37.0 37.0 27.4 37.0 135-139 34.75405 37.0 37.0 37.0 25.0 37.0 140-144 34.52375 37.0 37.0 37.0 25.0 37.0 145-149 34.15815 37.0 37.0 37.0 25.0 37.0 150-151 33.933 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 4.0 13 11.0 14 10.0 15 18.0 16 8.0 17 5.0 18 1.0 19 7.0 20 3.0 21 8.0 22 20.0 23 24.0 24 25.0 25 31.0 26 43.0 27 32.0 28 25.0 29 19.0 30 20.0 31 50.0 32 70.0 33 103.0 34 227.0 35 441.0 36 2534.0 37 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.43963782696177 20.497987927565394 6.3128772635814885 20.74949698189135 2 36.425000000000004 22.025 21.975 19.575 3 30.125 24.099999999999998 24.775 21.0 4 31.7 28.875 18.05 21.375 5 32.95 30.775000000000002 17.575 18.7 6 30.375000000000004 32.025 18.075 19.525000000000002 7 28.675 20.5 28.525 22.3 8 28.825 20.325 23.05 27.800000000000004 9 29.5 21.475 23.400000000000002 25.624999999999996 10-14 30.955 24.58 20.43 24.035 15-19 31.2 23.825 22.0 22.975 20-24 31.45415436946626 23.110399679855938 21.714771647241257 23.720674303436546 25-29 31.48936170212766 23.81476846057572 21.536921151439298 23.15894868585732 30-34 30.35231708537684 23.421078971073968 22.44520068061255 23.781403262936642 35-39 30.118553349007055 24.120854384473013 22.380071031964384 23.38052123455555 40-44 30.95631286593605 23.735174898663864 21.778511734974728 23.53000050042536 45-49 28.810167116981887 24.54217952566797 23.53647553287301 23.11117782447713 50-54 29.02806262818268 23.505577509879448 23.465559501775797 24.00080036016207 55-59 31.193992490613265 23.709637046307886 22.29787234042553 22.798498122653317 60-64 31.239057575909158 23.000350157570907 21.80981441648742 23.950777850032516 65-69 30.19754938734684 23.945986496624155 22.445611402850712 23.410852713178297 70-74 30.683354192740925 23.644555694618273 22.62828535669587 23.04380475594493 75-79 29.070524050252764 24.110315831623204 22.753891586165473 24.065268531958555 80-84 29.66241560390098 24.191047761940485 22.920730182545636 23.225806451612904 85-89 31.142370845014018 23.30296355626752 23.0326391670004 22.52202643171806 90-94 30.90854512707625 23.949369621773066 22.198318991394835 22.943766259755854 95-99 31.374118353258968 24.59106597969086 22.490120554249412 21.54469511280076 100-104 31.919899874843555 24.0 22.367959949937422 21.712140175219023 105-109 31.854818523153945 23.83979974968711 22.513141426783477 21.79224030037547 110-114 32.274523535591015 24.01580711320094 22.260017007653442 21.449652343554597 115-119 32.94117647058823 23.46433041301627 22.287859824780977 21.306633291614517 120-124 32.76595744680851 23.684605757196493 22.708385481852318 20.84105131414268 125-129 32.95960758796737 23.890084588818258 22.278392311927526 20.87191551128685 130-134 33.65707133917397 23.429286608260323 22.18773466833542 20.72590738423029 135-139 33.9874843554443 23.038798498122652 22.11764705882353 20.856070087609513 140-144 35.53599119603822 23.32049422240008 21.359611825321394 19.783902756240305 145-149 36.13016270337923 21.712140175219023 21.892365456821025 20.265331664580728 150-151 35.98247809762203 22.07759699624531 23.416770963704632 18.523153942428035 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.5 8 1.5 9 1.5 10 0.5 11 0.5 12 1.0 13 0.5 14 1.0 15 1.5 16 1.0 17 0.5 18 1.0 19 1.5 20 1.0 21 1.5 22 1.0 23 0.0 24 0.5 25 1.0 26 2.5 27 3.0 28 2.0 29 1.0 30 0.0 31 1.5 32 1.5 33 3.5 34 10.0 35 20.5 36 19.5 37 25.0 38 44.0 39 54.0 40 70.0 41 102.5 42 117.0 43 112.5 44 157.5 45 177.0 46 137.0 47 156.5 48 198.5 49 195.0 50 183.0 51 175.5 52 160.5 53 145.5 54 123.0 55 105.0 56 111.0 57 101.5 58 107.0 59 116.5 60 86.5 61 71.0 62 78.5 63 63.5 64 52.5 65 48.5 66 46.5 67 57.0 68 49.5 69 44.5 70 45.5 71 39.0 72 36.0 73 38.5 74 22.5 75 8.0 76 13.5 77 21.0 78 17.5 79 7.5 80 7.0 81 8.0 82 5.5 83 2.5 84 1.5 85 2.0 86 5.0 87 6.0 88 3.5 89 6.0 90 7.0 91 4.5 92 9.0 93 14.0 94 16.5 95 18.5 96 15.0 97 14.0 98 15.5 99 12.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.045 25-29 0.125 30-34 0.09 35-39 0.045 40-44 0.08499999999999999 45-49 0.06999999999999999 50-54 0.045 55-59 0.125 60-64 0.045 65-69 0.025 70-74 0.125 75-79 0.105 80-84 0.025 85-89 0.12 90-94 0.06 95-99 0.045 100-104 0.125 105-109 0.125 110-114 0.045 115-119 0.125 120-124 0.125 125-129 0.105 130-134 0.125 135-139 0.125 140-144 0.045 145-149 0.125 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.325 #Duplication Level Percentage of deduplicated Percentage of total 1 72.12405337179949 50.0 2 17.526144969347275 24.3 3 6.887847097006852 14.325 4 2.307969707897584 6.4 5 0.7573025604038947 2.625 6 0.288496213487198 1.2 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.10818608005769925 1.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 25 0.625 No Hit GAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGCAG 11 0.27499999999999997 No Hit GTCCCAACCGCAAAACCCTAGCCGCCGCTCTCCCTGCTCGCCGCCGCCGG 10 0.25 No Hit GGGCCATCGAGTTCACCAAGTAGATCAAATCATCCATCCATTTGAGATTA 6 0.15 No Hit CTTGTTTTCACTTGGCTACAAAATATAGATCTAGTTATGTCTATATAGTG 6 0.15 No Hit AGATAATGGAAAATGGTTGCCACAACATTCAGTGTGCAATATTATGTAGA 6 0.15 No Hit AGAGGAACCGTTCATTCAGATAATTGGTTTACGCGGCTGTCTGATCAGGC 6 0.15 No Hit GTGAAATTGATGGTCTTCAATCCAATGGAGTCAGTTGCGATGGAAGAATA 6 0.15 No Hit AGGGCGAACGCGAGCCGCTACGGATTGGCCGCCGGTGTTTTCACCAACAA 6 0.15 No Hit CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC 6 0.15 No Hit GGGCAGCAAGGTGCCGCTCTACGGCGGCGGCGCCGGGCTGAGCAGCACCC 6 0.15 No Hit GGGAGACTGGCCGTTCCCGCGGGTTCGGCTTCGTCACCTTCGCCAGCGAG 5 0.125 No Hit GAACTGATTTCATGTTTACCTCTTCACTTTGAGCCCGAGAAAGATTGTGC 5 0.125 No Hit GGACGGCGCTCCGGAACGCCGAGGGGTTCGGCCTGTTCGCCTCGGTCCAC 5 0.125 No Hit GGCAGAGGGAAGCCACAGCCATTGCTTGCAGGTTTGTGCGCCGTGGAGCC 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 5 0.125 No Hit AGCATACCTCTGGCATTTGGATCTGCCCCATTCTCCAGAAGCAGCTTGAC 5 0.125 No Hit GATCGATGGAAGAAAAAGGCGGCGGTGGCCTGGCTTATTACTGCCTGTTC 5 0.125 No Hit GAAAACCCTGCTACCTGGGGTGCTGGTATGGGTAACAGCTGGAGAACAAC 5 0.125 No Hit GCTCACCGTGCTGTTTATCCTCCGCCCCTTTCTTGCCGTTGCCTTCGTCA 5 0.125 No Hit GTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTAT 5 0.125 No Hit GCTTGGATGCCTTCCACCATTTCCACGAGCACCGAAGAATATTTACCACC 5 0.125 No Hit GACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTG 5 0.125 No Hit AGCTAGCAAGCGGCGCCTGCGCCCGCCGCCTGCCCCGACCCACGTTAGGG 5 0.125 No Hit ACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGAA 5 0.125 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 5 0.125 No Hit GAGTCTTACAGAGATTCTCATCCCAGAGCCCCTGTTGTTGTGCCCTGGAT 5 0.125 No Hit GTGAACATCATGTATCAATACAACATCATTCTTGGCCGGGTGTACCTCAA 5 0.125 No Hit GCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCG 5 0.125 No Hit GAGACGCAGTCGAGTCAACTCCAATCCTTACTCCTCCTCCTCCTGCCCCG 5 0.125 No Hit GGGAGTACAGGGGAGCTAGATCCAGAGAGAAGTAAAATGATCCGAAGGCT 5 0.125 No Hit CACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.037500000000000006 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.0625 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.175 0.0 0.0 0.0 0.0 64-65 0.2 0.0 0.0 0.0 0.0 66-67 0.3125 0.0 0.0 0.0 0.0 68-69 0.35 0.0 0.0 0.0 0.0 70-71 0.3875 0.0 0.0 0.0 0.0 72-73 0.4125 0.0 0.0 0.0 0.0 74-75 0.5125 0.0 0.0 0.0 0.0 76-77 0.9125 0.0 0.0 0.0 0.0 78-79 1.1375000000000002 0.0 0.0 0.0 0.0 80-81 1.25 0.0 0.0 0.0 0.0 82-83 1.525 0.0 0.0 0.0 0.0 84-85 1.775 0.0 0.0 0.0 0.0 86-87 2.0625 0.0 0.0 0.0 0.0 88-89 2.7249999999999996 0.0 0.0 0.0 0.0 90-91 3.0875 0.0 0.0 0.0 0.0 92-93 3.375 0.0 0.0 0.0 0.0 94-95 3.9375 0.0 0.0 0.0 0.0 96-97 4.65 0.0 0.0 0.0 0.0 98-99 5.25 0.0 0.0 0.0 0.0 100-101 6.0625 0.0 0.0 0.0 0.0 102-103 6.775 0.0 0.0 0.0 0.0 104-105 7.5375 0.0 0.0 0.0 0.0 106-107 8.1875 0.0 0.0 0.0 0.0 108-109 8.787500000000001 0.0 0.0 0.0 0.0 110-111 9.5375 0.0 0.0 0.0 0.0 112-113 10.2625 0.0 0.0 0.0 0.0 114-115 10.9625 0.0 0.0 0.0 0.0 116-117 11.7125 0.0 0.0 0.0 0.0 118-119 12.8 0.0 0.0 0.0 0.0 120-121 13.75 0.0 0.0 0.0 0.0 122-123 14.575 0.0 0.0 0.0 0.0 124-125 15.3375 0.0 0.0 0.0 0.0 126-127 16.15 0.0 0.0 0.0 0.0 128-129 17.0125 0.0 0.0 0.0 0.0 130-131 18.1125 0.0 0.0 0.0 0.0 132-133 18.762500000000003 0.0 0.0 0.0 0.0 134-135 19.875 0.0 0.0 0.0 0.0 136-137 20.799999999999997 0.0 0.0 0.0 0.0 138-139 21.799999999999997 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027795 spots for SRR13165357.sra Written 1027795 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra Read 1027786 spots for SRR13165357.sra Written 1027786 spots for SRR13165357.sra SRR ids: ['SRR13165357.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__21s2kxo SRR13165357.sra spots: 20555729 blocks: [[1, 1027786], [1027787, 2055572], [2055573, 3083358], [3083359, 4111144], [4111145, 5138930], [5138931, 6166716], [6166717, 7194502], [7194503, 8222288], [8222289, 9250074], [9250075, 10277860], [10277861, 11305646], [11305647, 12333432], [12333433, 13361218], [13361219, 14389004], [14389005, 15416790], [15416791, 16444576], [16444577, 17472362], [17472363, 18500148], [18500149, 19527934], [19527935, 20555729]] SRR13165357 file size 6964035 SRR13165357 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165357 SRR13165357_1.fastq SRR13165357_2.fastq Input file: SRR13165357_1.fastq Paired file: SRR13165357_2.fastq trimmed: SRR13165357-trimmed-pair1.fastq, SRR13165357-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:01:48 2024 >> started Sat Dec 7 16:02:31 2024 >> done (43.112s) 20555729 read pairs processed; of these: 583 ( 0.00%) short read pairs filtered out after trimming by size control 772027 ( 3.76%) empty read pairs filtered out after trimming by size control 19783119 (96.24%) read pairs available; of these: 5588289 (28.25%) trimmed read pairs available after processing 14194830 (71.75%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 39 0.00% 19 66 0.00% 20 117 0.00% 21 93 0.00% 22 178 0.00% 23 226 0.00% 24 311 0.00% 25 220 0.00% 26 284 0.00% 27 331 0.00% 28 455 0.00% 29 424 0.00% 30 600 0.00% 31 513 0.00% 32 615 0.00% 33 646 0.00% 34 581 0.00% 35 651 0.00% 36 735 0.00% 37 744 0.00% 38 976 0.00% 39 798 0.00% 40 1064 0.01% 41 1061 0.01% 42 1015 0.01% 43 1260 0.01% 44 1262 0.01% 45 1274 0.01% 46 1354 0.01% 47 1476 0.01% 48 1828 0.01% 49 1733 0.01% 50 1821 0.01% 51 2183 0.01% 52 2368 0.01% 53 2702 0.01% 54 2698 0.01% 55 2858 0.01% 56 2730 0.01% 57 3439 0.02% 58 3452 0.02% 59 4024 0.02% 60 4171 0.02% 61 4912 0.02% 62 4763 0.02% 63 5939 0.03% 64 6264 0.03% 65 6219 0.03% 66 6619 0.03% 67 7038 0.04% 68 7795 0.04% 69 8295 0.04% 70 9185 0.05% 71 10794 0.05% 72 11689 0.06% 73 12568 0.06% 74 14629 0.07% 75 15164 0.08% 76 15911 0.08% 77 17195 0.09% 78 17909 0.09% 79 20436 0.10% 80 21143 0.11% 81 23793 0.12% 82 25921 0.13% 83 28510 0.14% 84 30676 0.16% 85 32338 0.16% 86 35390 0.18% 87 36805 0.19% 88 38411 0.19% 89 39027 0.20% 90 42430 0.21% 91 45182 0.23% 92 46180 0.23% 93 51686 0.26% 94 52843 0.27% 95 56662 0.29% 96 57367 0.29% 97 59815 0.30% 98 62136 0.31% 99 65389 0.33% 100 64929 0.33% 101 65747 0.33% 102 66202 0.33% 103 67655 0.34% 104 69284 0.35% 105 69447 0.35% 106 71478 0.36% 107 71657 0.36% 108 71594 0.36% 109 75536 0.38% 110 76410 0.39% 111 77405 0.39% 112 77923 0.39% 113 78419 0.40% 114 81799 0.41% 115 83515 0.42% 116 85307 0.43% 117 83732 0.42% 118 82832 0.42% 119 85597 0.43% 120 87168 0.44% 121 85627 0.43% 122 86736 0.44% 123 88510 0.45% 124 90946 0.46% 125 90982 0.46% 126 92665 0.47% 127 92015 0.47% 128 92635 0.47% 129 94882 0.48% 130 92053 0.47% 131 94063 0.48% 132 95059 0.48% 133 95932 0.48% 134 96609 0.49% 135 98354 0.50% 136 100025 0.51% 137 97446 0.49% 138 99696 0.50% 139 98127 0.50% 140 98811 0.50% 141 100920 0.51% 142 100730 0.51% 143 101428 0.51% 144 104025 0.53% 145 101376 0.51% 146 102719 0.52% 147 99386 0.50% 148 97722 0.49% 149 98459 0.50% 150 96311 0.49% 151 14194830 71.75% 19783119 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=21.64 fanout-score-rank=6 prefix-density=0.29 prefix-fanout=21.6 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGATCATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=25 fanout-score=130.16 fanout-score-rank=1 prefix-density=0.78 prefix-fanout=13.0 sequence=CCTTCTTCTTCTG criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.42 fanout-score-rank=34 prefix-density=0.44 prefix-fanout=2.3 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.09 sequence-density-rank=17 fanout-score=211.86 fanout-score-rank=1 prefix-density=0.81 prefix-fanout=24.0 sequence=GAAGAAGAAGAGG SRR13165357 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:03:50 Started mapping on | Dec 07 16:03:50 Finished on | Dec 07 16:06:30 Mapping speed, Million of reads per hour | 445.12 Number of input reads | 19783119 Average input read length | 283 UNIQUE READS: Uniquely mapped reads number | 15462987 Uniquely mapped reads % | 78.16% Average mapped length | 284.35 Number of splices: Total | 11568987 Number of splices: Annotated (sjdb) | 10759652 Number of splices: GT/AG | 11380984 Number of splices: GC/AG | 152434 Number of splices: AT/AC | 8865 Number of splices: Non-canonical | 26704 Mismatch rate per base, % | 0.26% Deletion rate per base | 0.02% Deletion average length | 2.13 Insertion rate per base | 0.02% Insertion average length | 2.24 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 291909 % of reads mapped to multiple loci | 1.48% Number of reads mapped to too many loci | 619039 % of reads mapped to too many loci | 3.13% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.09% % of reads unmapped: other | 12.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4028420 4028420 4028420 N_multimapping 291909 291909 291909 N_noFeature 435246 14942972 584780 N_ambiguous 428773 1910 58525 UnstrandedReadsAssigned:14598968 PositiveStrandReadsAssigned:518105 NegativeStrandReadsAssigned:14819682 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=135 echo kmer=131 SRR13165357 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165357-trimmed-pair1.fastq SRR13165357-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,783,119 reads, 15,233,226 reads pseudoaligned [quant] estimated average fragment length: 212.141 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,191 rounds 52973 SRR13165357.ke.tsv 35125 SRR13165357.se.tsv 88098 total ==> SRR13165357.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 725.05 0 0 PNS24247 1044 832.859 52.7344 5.28307 PNS24249 1928 1716.86 82.8305 4.0255 PNS24246 1044 832.859 52.7344 5.28307 PNS24248 1044 832.859 52.7344 5.28307 PNS24244 1471 1259.86 435.966 28.8732 PNS24243 293 118.268 1 0.705497 KQK14069 1603 1391.86 18102 1085.16 KQK14071 474 272.222 153.157 46.9439 ==> SRR13165357.se.tsv <== BRADI_1g14170v3 18357 BRADI_1g53295v3 305 BRADI_1g59795v3 552 BRADI_1g07683v3 0 BRADI_1g00485v3 13 BRADI_1g20270v3 612 BRADI_1g74790v3 352 BRADI_1g09890v3 0 BRADI_1g77505v3 467 BRADI_1g48960v3 0 SRR13165357 completed mapping pipeline successfully