Starting /dee2/code/volunteer_pipeline.sh SRR13165359 current disk space = 1542054031360 free memory = 1461970308 SRR13165359 SRAfilesize ad1b4010a80bc7cde2f7f8f8178c28ba SRR13165359.sra SRR13165359.sra file validated SRR13165359 is paired end SRR13165359 is conventional basespace SRR13165359 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165359_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5395 37.0 37.0 37.0 37.0 37.0 2 36.14275 37.0 37.0 37.0 37.0 37.0 3 36.577 37.0 37.0 37.0 37.0 37.0 4 36.593 37.0 37.0 37.0 37.0 37.0 5 36.5775 37.0 37.0 37.0 37.0 37.0 6 36.625 37.0 37.0 37.0 37.0 37.0 7 36.5085 37.0 37.0 37.0 37.0 37.0 8 36.634 37.0 37.0 37.0 37.0 37.0 9 36.535 37.0 37.0 37.0 37.0 37.0 10-14 36.5712 37.0 37.0 37.0 37.0 37.0 15-19 36.507 37.0 37.0 37.0 37.0 37.0 20-24 36.5043 37.0 37.0 37.0 37.0 37.0 25-29 36.48700000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.449 37.0 37.0 37.0 37.0 37.0 35-39 36.4168 37.0 37.0 37.0 37.0 37.0 40-44 36.382600000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.2417 37.0 37.0 37.0 37.0 37.0 50-54 36.3155 37.0 37.0 37.0 37.0 37.0 55-59 36.2049 37.0 37.0 37.0 37.0 37.0 60-64 36.2667 37.0 37.0 37.0 37.0 37.0 65-69 36.107600000000005 37.0 37.0 37.0 37.0 37.0 70-74 36.1609 37.0 37.0 37.0 37.0 37.0 75-79 36.2741 37.0 37.0 37.0 37.0 37.0 80-84 36.1971 37.0 37.0 37.0 37.0 37.0 85-89 36.1999 37.0 37.0 37.0 37.0 37.0 90-94 36.1149 37.0 37.0 37.0 37.0 37.0 95-99 36.1254 37.0 37.0 37.0 37.0 37.0 100-104 36.1075 37.0 37.0 37.0 37.0 37.0 105-109 36.1493 37.0 37.0 37.0 37.0 37.0 110-114 36.082499999999996 37.0 37.0 37.0 37.0 37.0 115-119 36.0214 37.0 37.0 37.0 37.0 37.0 120-124 35.9803 37.0 37.0 37.0 37.0 37.0 125-129 35.8056 37.0 37.0 37.0 37.0 37.0 130-134 35.7324 37.0 37.0 37.0 37.0 37.0 135-139 35.6276 37.0 37.0 37.0 37.0 37.0 140-144 35.4975 37.0 37.0 37.0 37.0 37.0 145-149 35.110200000000006 37.0 37.0 37.0 29.8 37.0 150-151 34.85 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 0.0 20 1.0 21 0.0 22 1.0 23 3.0 24 1.0 25 1.0 26 4.0 27 9.0 28 20.0 29 13.0 30 26.0 31 41.0 32 68.0 33 90.0 34 183.0 35 378.0 36 2767.0 37 392.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.475 8.774999999999999 9.125 40.625 2 25.939944486500128 12.414837244511734 28.8922533434267 32.75296492556144 3 21.6 17.65 23.25 37.5 4 26.825 23.025000000000002 18.375 31.775 5 29.25 27.750000000000004 20.225 22.775000000000002 6 25.674999999999997 33.15 19.825 21.349999999999998 7 19.25 27.1 34.725 18.925 8 23.525 25.724999999999998 24.75 26.0 9 20.05 24.625 29.575000000000003 25.75 10-14 23.849999999999998 27.284999999999997 24.060000000000002 24.805 15-19 23.35 25.6 25.215 25.835 20-24 23.735 25.72 24.64 25.905 25-29 24.075 25.525 24.79 25.61 30-34 24.565 25.255 24.055 26.125 35-39 23.965 26.090000000000003 24.060000000000002 25.885 40-44 24.04 25.69 24.0 26.27 45-49 24.355 25.705 23.525 26.415 50-54 24.224999999999998 25.564999999999998 23.935000000000002 26.275 55-59 24.695 25.119999999999997 23.955000000000002 26.229999999999997 60-64 23.75 25.569999999999997 24.79 25.89 65-69 24.855 26.52 23.11 25.515 70-74 25.045 25.44 23.62 25.895000000000003 75-79 25.585 25.355 23.52 25.540000000000003 80-84 24.5 25.215 24.01 26.275 85-89 24.93 25.4 23.375 26.295 90-94 25.585 25.385 23.150000000000002 25.88 95-99 25.605 24.585 23.64 26.169999999999998 100-104 25.965 25.665 22.235 26.135 105-109 25.355 25.419999999999998 22.825 26.400000000000002 110-114 26.419999999999998 25.55 21.78 26.25 115-119 26.119999999999997 26.584999999999997 22.06 25.235000000000003 120-124 25.455 25.64 23.044999999999998 25.86 125-129 25.900000000000002 25.014999999999997 22.665 26.419999999999998 130-134 27.150000000000002 24.465 22.545 25.840000000000003 135-139 25.590000000000003 24.099999999999998 24.02 26.290000000000003 140-144 26.55 24.185000000000002 23.25 26.015 145-149 27.015 24.33 23.235 25.419999999999998 150-151 26.174999999999997 23.6125 24.25 25.9625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 1.0 25 1.0 26 0.0 27 0.0 28 1.0 29 1.5 30 1.0 31 5.5 32 13.5 33 19.0 34 24.0 35 36.0 36 57.0 37 75.5 38 83.0 39 90.5 40 99.0 41 138.0 42 166.5 43 155.5 44 158.0 45 159.5 46 163.0 47 183.0 48 186.0 49 166.0 50 164.5 51 163.5 52 139.5 53 122.0 54 122.5 55 104.0 56 86.0 57 92.5 58 93.5 59 82.0 60 64.5 61 67.5 62 64.5 63 54.5 64 60.5 65 72.5 66 69.5 67 58.5 68 52.5 69 41.0 70 34.5 71 33.5 72 33.0 73 33.0 74 28.0 75 16.0 76 12.0 77 13.0 78 11.0 79 11.0 80 8.0 81 2.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.9249999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.02499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59215550156196 53.725 2 17.459215550156195 25.15 3 5.206525511975009 11.25 4 1.5966678236723362 4.6 5 0.8330440819160014 3.0 6 0.10413051023950018 0.44999999999999996 7 0.03471017007983339 0.17500000000000002 8 0.06942034015966678 0.4 9 0.03471017007983339 0.22499999999999998 >10 0.06942034015966678 1.0250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGGCTATCTCGTAT 30 0.75 TruSeq Adapter, Index 9 (97% over 36bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGGCTATCGCGTAT 11 0.27499999999999997 TruSeq Adapter, Index 9 (97% over 36bp) TCTGCTACCTCGATGAGATTTTTGATCTGTGCAACCCTCTTAGACACTTC 9 0.22499999999999998 No Hit GTTCACCGCGGACCCGCCGCCGACCGTGGAGCCCGTGAACACCATGGTCG 8 0.2 No Hit CAAGTAGTTGACTTATATTGACCAGTTGTAATTGCAACCGTGCGGCAAAC 8 0.2 No Hit ACGGAACTTGAGAGCATGGAAAGCTACCCTGCACCTAAGTCTTTGAAGTT 7 0.17500000000000002 No Hit CTCTGCCACTCCCAACTTATGTTTCTTATTGCTACCGACACACAGACAGC 6 0.15 No Hit CCTGGATGGTCCAACACCTACAAACATTTCCATGAAATCGGAACCAGAAA 6 0.15 No Hit TGCCAAGAAGCCAATTTGCATGCAGAGTTTTTCGGTAGGACCAGCTAAAC 6 0.15 No Hit GCGAGAGAGTGAGAGAGAGAGGGAGAAGAAGTGACGAATCGAAGCACAAC 5 0.125 No Hit GCTATATGGAAGAGACCACATGATGATACTCTAGCTGTAAACCATTCACC 5 0.125 No Hit GGCATGCCATAAAATTCATCGTATGTAACACATATTCACCCGATTATCGT 5 0.125 No Hit TCACGGTCAAATGCAAGGTTCATCCCGCACATCGGAAACAAGGTGCCCTT 5 0.125 No Hit GCGAGAAGAGGCTGCTTTGCTTCGTCCCACTTGTCTAGTCTTCTCCTCGC 5 0.125 No Hit TGAACAGAAAAGAGTATGAACCCTCGGTCTTAAATTCACGCCTGCCAGAC 5 0.125 No Hit GCACCCAGTAGCATAGTTCATCACCAATAACCCATGTTCCCTACACACAT 5 0.125 No Hit TACAGGTACAACCCCGATACAACAGAGAGAAGAACTAAAGGAAAAACTAG 5 0.125 No Hit AGCACGAGTAGATAGTGTCCTAACAACCTCTCCTTTGGTCATGTATAGCT 5 0.125 No Hit GCTGTCTCTCTTCTAGTAGAAGGGCCTCCCCTTCTTTTACAGTCTCTTCT 5 0.125 No Hit CATGCCACCAGGAGGAGGAGCAGCAGCAGCAAGCACGCGGAGGCGAGGGC 5 0.125 No Hit GTCGAGAAATGGGTAAGGCCACCAGGATAAACCTCCGCAAGCATGAAGAA 5 0.125 No Hit AGGCACCTTCCCCTCTGGGTTGATCTTCAGGAACCACTCGGGCTTGTTGC 5 0.125 No Hit GTCCACAACATGTGCATCCCTCACTCTCTCCAACATAAACCCACAAATAA 5 0.125 No Hit CAGACACAACCACTTCATTTCTCTTTATTATTTTCTTACTAGTATCTATC 5 0.125 No Hit CCTCGATCGGCCACACCTGCATGCAGCTGATCCTTCCGCCGTTGCTGACG 5 0.125 No Hit GTCCCAATAGCCCAGACAGGCTCATATGCAATTACAACGTCTGTCCAGTT 5 0.125 No Hit GCACGGGTGGATGTTCTGTTAGCCGGTCCGTGCAGAGCATTGTGCCTGAG 5 0.125 No Hit CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC 5 0.125 No Hit CTGACACGAAATTACATTAATCACCTGGCTGGAGAGCCGCCGGCGCACGG 5 0.125 No Hit GGGTGCTCCTTGGTGACGCAGCTGGCGAGCATCCTGGTGCCGTCGAGCGG 5 0.125 No Hit CCATGCTTATGGAAGTAGTTGTGGTTGTAGTGGTGCTTGAATCAATTGAC 5 0.125 No Hit GCCACCTGAGGATTTATCTTGTCGAGCTGTAAGACAACTTCACCCAGGAA 5 0.125 No Hit GTCCTCAACGCCATTGATATCTTTGTTCTCGAGCACTGCTCCATCTGGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0125 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.037500000000000006 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.125 0.0 0.0 0.0 0.0 50-51 0.15 0.0 0.0 0.0 0.0 52-53 0.15 0.0 0.0 0.0 0.0 54-55 0.1875 0.0 0.0 0.0 0.0 56-57 0.21250000000000002 0.0 0.0 0.0 0.0 58-59 0.275 0.0 0.0 0.0 0.0 60-61 0.325 0.0 0.0 0.0 0.0 62-63 0.45 0.0 0.0 0.0 0.0 64-65 0.575 0.0 0.0 0.0 0.0 66-67 0.6875 0.0 0.0 0.0 0.0 68-69 0.825 0.0 0.0 0.0 0.0 70-71 1.0125 0.0 0.0 0.0 0.0 72-73 1.125 0.0 0.0 0.0 0.0 74-75 1.2875 0.0 0.0 0.0 0.0 76-77 1.5875 0.0 0.0 0.0 0.0 78-79 1.8 0.0 0.0 0.0 0.0 80-81 2.175 0.0 0.0 0.0 0.0 82-83 2.6875 0.0 0.0 0.0 0.0 84-85 3.0875000000000004 0.0 0.0 0.0 0.0 86-87 3.4875 0.0 0.0 0.0 0.0 88-89 4.112500000000001 0.0 0.0 0.0 0.0 90-91 4.612500000000001 0.0 0.0 0.0 0.0 92-93 5.4125 0.0 0.0 0.0 0.0 94-95 6.1 0.0 0.0 0.0 0.0 96-97 7.1 0.0 0.0 0.0 0.0 98-99 8.0 0.0 0.0 0.0 0.0 100-101 8.8625 0.0 0.0 0.0 0.0 102-103 9.6875 0.0 0.0 0.0 0.0 104-105 10.587499999999999 0.0 0.0 0.0 0.0 106-107 11.6625 0.0 0.0 0.0 0.0 108-109 12.4375 0.0 0.0 0.0 0.0 110-111 13.3 0.0 0.0 0.0 0.0 112-113 14.3125 0.0 0.0 0.0 0.0 114-115 15.3875 0.0 0.0 0.0 0.0 116-117 16.6875 0.0 0.0 0.0 0.0 118-119 17.762500000000003 0.0 0.0 0.0 0.0 120-121 18.85 0.0 0.0 0.0 0.0 122-123 19.5375 0.0 0.0 0.0 0.0 124-125 20.55 0.0 0.0 0.0 0.0 126-127 21.3375 0.0 0.0 0.0 0.0 128-129 22.0375 0.0 0.0 0.0 0.0 130-131 22.887500000000003 0.0 0.0 0.0 0.0 132-133 23.625 0.0 0.0 0.0 0.0 134-135 24.4875 0.0 0.0 0.0 0.0 136-137 25.2375 0.0 0.0 0.0 0.0 138-139 25.85 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTCCT 10 0.006830828 145.0 8 GGGGGGG 275 2.922292E-6 7.9090905 140-144 >>END_MODULE SRR13165359 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165359_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9885 37.0 37.0 37.0 37.0 37.0 2 36.238 37.0 37.0 37.0 37.0 37.0 3 36.193 37.0 37.0 37.0 37.0 37.0 4 36.309 37.0 37.0 37.0 37.0 37.0 5 36.303 37.0 37.0 37.0 37.0 37.0 6 36.297 37.0 37.0 37.0 37.0 37.0 7 36.327 37.0 37.0 37.0 37.0 37.0 8 36.2845 37.0 37.0 37.0 37.0 37.0 9 36.1595 37.0 37.0 37.0 37.0 37.0 10-14 36.17959999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.094899999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.07145 37.0 37.0 37.0 37.0 37.0 25-29 35.88485000000001 37.0 37.0 37.0 37.0 37.0 30-34 35.85965 37.0 37.0 37.0 37.0 37.0 35-39 35.79475 37.0 37.0 37.0 37.0 37.0 40-44 35.82815000000001 37.0 37.0 37.0 37.0 37.0 45-49 35.774150000000006 37.0 37.0 37.0 37.0 37.0 50-54 35.78365 37.0 37.0 37.0 37.0 37.0 55-59 35.839549999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.83655 37.0 37.0 37.0 37.0 37.0 65-69 35.7692 37.0 37.0 37.0 37.0 37.0 70-74 35.63395 37.0 37.0 37.0 37.0 37.0 75-79 35.599149999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.660000000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.66995 37.0 37.0 37.0 37.0 37.0 90-94 35.7066 37.0 37.0 37.0 37.0 37.0 95-99 35.7582 37.0 37.0 37.0 37.0 37.0 100-104 35.655150000000006 37.0 37.0 37.0 37.0 37.0 105-109 35.5924 37.0 37.0 37.0 37.0 37.0 110-114 35.47975 37.0 37.0 37.0 37.0 37.0 115-119 35.30355 37.0 37.0 37.0 37.0 37.0 120-124 35.09355 37.0 37.0 37.0 29.8 37.0 125-129 34.9642 37.0 37.0 37.0 27.4 37.0 130-134 34.73455 37.0 37.0 37.0 25.0 37.0 135-139 34.479549999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.257549999999995 37.0 37.0 37.0 25.0 37.0 145-149 33.89375 37.0 37.0 37.0 25.0 37.0 150-151 33.374375 37.0 37.0 37.0 18.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 4.0 14 7.0 15 7.0 16 5.0 17 5.0 18 7.0 19 2.0 20 3.0 21 11.0 22 8.0 23 5.0 24 7.0 25 6.0 26 14.0 27 24.0 28 23.0 29 23.0 30 25.0 31 49.0 32 100.0 33 131.0 34 269.0 35 547.0 36 2482.0 37 234.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.777050830397584 16.960241570206342 13.89028686462003 30.372420734776046 2 33.275 21.7 23.150000000000002 21.875 3 26.325 23.45 24.95 25.275 4 28.625 28.95 20.674999999999997 21.75 5 30.8 27.725 18.55 22.925 6 27.025 30.95 19.6 22.425 7 24.55 17.549999999999997 33.475 24.425 8 27.025 21.325 22.475 29.175 9 26.025 20.875 26.875 26.224999999999998 10-14 28.005000000000003 25.014999999999997 21.43 25.55 15-19 27.384999999999998 24.095 23.89 24.63 20-24 26.76401460219033 24.298644796719508 23.038455768365253 25.89888483272491 25-29 27.615711783837877 24.048036027020263 23.432574430823117 24.90367775831874 30-34 27.212245510479715 24.586063728677903 23.205442449102094 24.99624831174028 35-39 26.989048357253587 23.838575786367954 23.908586287943194 25.263789568435264 40-44 27.14721624731129 24.185883647641436 23.275473963283478 25.391426141763795 45-49 26.94943230130546 24.033411694092933 24.37853248637023 24.63862351823138 50-54 27.189078361754266 24.113617042556385 24.1186177926689 24.578686803020453 55-59 27.280460345258945 23.60270202651989 23.892919689767325 25.223917938453837 60-64 27.279091863779563 24.093614042106314 23.57353603040456 25.05375806370956 65-69 27.525 23.849999999999998 23.79 24.834999999999997 70-74 27.06529897423067 24.028021015761823 23.9729797348011 24.933700275206405 75-79 27.890339686827755 24.078243033668517 24.468457651708437 23.562959627795287 80-84 27.589999999999996 24.14 23.45 24.82 85-89 27.84309801370891 24.776104467904137 22.629709311052185 24.751088207334767 90-94 27.900580116023203 24.8249649929986 23.179635927185437 24.094818963792758 95-99 28.16781678167817 23.887388738873888 23.62736273627363 24.317431743174318 100-104 29.787340505379035 24.55841881411058 22.516887665749312 23.137353014761068 105-109 28.88521965375763 24.80236165315721 23.58150705493846 22.730911638146704 110-114 31.11466720008001 25.118767815172276 22.013301995299294 21.75326298944842 115-119 29.81938259868915 25.466553259618752 22.11437434332316 22.599689798368942 120-124 30.71303477608206 24.878658994245683 23.002251688766577 21.40605454090568 125-129 32.31438863317991 24.249549729837902 22.68861316790074 20.747448469081448 130-134 32.27743582044738 23.935345043286794 23.439923935345046 20.347295200920783 135-139 33.3800350262697 23.592694520890667 23.532649487115336 19.494620965724295 140-144 34.615192278841825 23.49352402860429 22.608391258688805 19.28289243386508 145-149 35.236427320490364 21.806354766074556 22.972229171878908 19.98498874155617 150-151 36.04403853371701 21.919179281871635 22.294507694232454 19.742274490178907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.5 6 1.0 7 1.0 8 1.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.0 16 0.5 17 0.0 18 0.5 19 0.5 20 0.5 21 0.5 22 0.0 23 0.0 24 0.0 25 2.0 26 2.5 27 1.0 28 1.5 29 4.5 30 6.0 31 7.0 32 14.5 33 22.0 34 27.0 35 26.0 36 26.5 37 38.0 38 48.5 39 66.0 40 94.0 41 117.5 42 129.5 43 142.5 44 157.5 45 166.5 46 166.0 47 176.0 48 191.5 49 169.0 50 143.0 51 142.5 52 145.0 53 129.5 54 113.0 55 97.0 56 76.0 57 75.0 58 79.0 59 84.0 60 92.0 61 96.0 62 99.5 63 85.0 64 72.5 65 76.0 66 64.5 67 51.5 68 61.5 69 64.5 70 54.5 71 46.5 72 40.5 73 34.5 74 29.0 75 23.0 76 13.0 77 9.0 78 6.5 79 6.0 80 6.0 81 3.0 82 2.0 83 4.0 84 4.5 85 3.0 86 2.0 87 1.0 88 0.5 89 2.0 90 3.5 91 3.5 92 3.5 93 4.0 94 4.0 95 5.0 96 4.0 97 1.5 98 2.5 99 4.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.65 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.015 25-29 0.075 30-34 0.045 35-39 0.015 40-44 0.045 45-49 0.034999999999999996 50-54 0.015 55-59 0.075 60-64 0.015 65-69 0.0 70-74 0.075 75-79 0.055 80-84 0.0 85-89 0.065 90-94 0.02 95-99 0.01 100-104 0.075 105-109 0.06999999999999999 110-114 0.015 115-119 0.065 120-124 0.075 125-129 0.06 130-134 0.08499999999999999 135-139 0.075 140-144 0.015 145-149 0.075 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.475 #Duplication Level Percentage of deduplicated Percentage of total 1 75.74004763525008 55.65 2 16.80843824430078 24.7 3 4.865600544402858 10.725 4 1.5651582170806397 4.6 5 0.6805035726437564 2.5 6 0.13610071452875128 0.6 7 0.06805035726437564 0.35000000000000003 8 0.10207553589656344 0.6 9 0.0 0.0 >10 0.03402517863218782 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 11 0.27499999999999997 No Hit CCACAGGGTGCAGTGCGACGCCGTGATCGTGGGTTCAGGCTGCGGCGGGG 8 0.2 No Hit AGGAACTGTCATCAGAGATGAGGTGGGCCTGTCACTTGACAAGGCAGACA 8 0.2 No Hit GCGGTACGACAGGCTGAGGAGCCTCGGGGCGCGGATACAGGAGATGGTCG 8 0.2 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 7 0.17500000000000002 No Hit CTTCAGAATATTACACCACAGAAGTCTTGCCCAGGCTTGCCAAATCAAAA 7 0.17500000000000002 No Hit AGCCTCTGATATCGAAGGACCATGAGCGTGCCTACTTCGACTCCGCAGAC 6 0.15 No Hit CAGCCTCTTCTCGCTTGCTCTACCTGCTGCTTGCAACCATGGCACCCACC 6 0.15 No Hit CACAAGAGTTGCTCACAGTGTAAAGTTCGCTCCTGGTGTGTGCTTATTAG 6 0.15 No Hit GCTGGCTGTGATGAAGCTAAACAAGAAATAATGGAGTTTGTACATTTCCT 6 0.15 No Hit GAGACAGCTGAGAAGTGTGTTGCTGATAATGGCATTGAGCGCCCTTCGAT 5 0.125 No Hit GTTCGAGGACCCGTTCAACACGTACCAGAGGATCGCCCTGTTTGATCACC 5 0.125 No Hit CCATCGCGTGGGCTGAGAAAAATATTGACAAGGATTGGAAGGCATGGACT 5 0.125 No Hit TGGAAATGTAGCTAATGTTCGAAAGTTGTTGTCCCATCCTGCTTTTGACA 5 0.125 No Hit TACTAATGAAGTGCAAAGTAACCAAGCAAACACAGTGGGCACTGATGCTC 5 0.125 No Hit GGCTTTGTTACACGCAATTCAGCTAGAACAAATCCATCAAATCCCTCAAT 5 0.125 No Hit GTTGGACTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACC 5 0.125 No Hit ATCCGTATATCCATCCATCGTTGGATTGATCCATTTATTCCCTTCTTTTA 5 0.125 No Hit GCAACTCGTAAATGGAATTTCATTCCTTTGATACCTATAAAACGATAATC 5 0.125 No Hit GTCGCCAGCCAAAACCCTCGCCGGAGTAGACCAGCCACAGCACTCCTCCC 5 0.125 No Hit GAAGGTGCTACTAGTAGCAGCGCGAGAGGAAAATGTTGCAGCAAAGAAAG 5 0.125 No Hit CTTTTCCCTGGTACCGGATGGCTTTCTTCGTATTCTGGAGCTGTGCCTAT 5 0.125 No Hit GCTTACCCAATTTGCAACATGTAATATTGAATGATAATCAACTAAACGAT 5 0.125 No Hit GTTCCAGATGAGCTTCGACTACGAGTCCATCTACGTGTCCGACTGGAGCA 5 0.125 No Hit GTCAAAATCTTAAGGTTATGGCATGCATAGGAGAACTGTTGGAAGAGAGG 5 0.125 No Hit CTGGGCCCTTCGGCAACGTGCAGTCGGAGGTGCTCATGCGCAAGGACGAC 5 0.125 No Hit ACTTCATCGGCGTGCCCCTTGGCGTACTCTTGGGATTCAAATTTGACTAT 5 0.125 No Hit GGCACTTGGGGAAGAAAGAACCAGGAAGGAACTGATTCCCTTTCTCAGTG 5 0.125 No Hit CTCGGCCAGCCAAAGCCAACCCATGGCGCACTCCCTCGCCGCCGTCTCCT 5 0.125 No Hit CAATGTTGATTGGTGCGAGTCTCACAAGCCGGACACCATACGTACCAATG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0125 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.037500000000000006 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.125 0.0 0.0 0.0 0.0 50-51 0.15 0.0 0.0 0.0 0.0 52-53 0.15 0.0 0.0 0.0 0.0 54-55 0.1875 0.0 0.0 0.0 0.0 56-57 0.21250000000000002 0.0 0.0 0.0 0.0 58-59 0.275 0.0 0.0 0.0 0.0 60-61 0.325 0.0 0.0 0.0 0.0 62-63 0.45 0.0 0.0 0.0 0.0 64-65 0.55 0.0 0.0 0.0 0.0 66-67 0.6625 0.0 0.0 0.0 0.0 68-69 0.8 0.0 0.0 0.0 0.0 70-71 0.9874999999999999 0.0 0.0 0.0 0.0 72-73 1.1 0.0 0.0 0.0 0.0 74-75 1.2625 0.0 0.0 0.0 0.0 76-77 1.5625 0.0 0.0 0.0 0.0 78-79 1.775 0.0 0.0 0.0 0.0 80-81 2.1875 0.0 0.0 0.0 0.0 82-83 2.7375 0.0 0.0 0.0 0.0 84-85 3.15 0.0 0.0 0.0 0.0 86-87 3.525 0.0 0.0 0.0 0.0 88-89 4.137499999999999 0.0 0.0 0.0 0.0 90-91 4.6625 0.0 0.0 0.0 0.0 92-93 5.45 0.0 0.0 0.0 0.0 94-95 6.125 0.0 0.0 0.0 0.0 96-97 7.15 0.0 0.0 0.0 0.0 98-99 8.075 0.0 0.0 0.0 0.0 100-101 8.9375 0.0 0.0 0.0 0.0 102-103 9.787500000000001 0.0 0.0 0.0 0.0 104-105 10.6875 0.0 0.0 0.0 0.0 106-107 11.7875 0.0 0.0 0.0 0.0 108-109 12.7 0.0 0.0 0.0 0.0 110-111 13.55 0.0 0.0 0.0 0.0 112-113 14.55 0.0 0.0 0.0 0.0 114-115 15.625 0.0 0.0 0.0 0.0 116-117 16.875 0.0 0.0 0.0 0.0 118-119 17.95 0.0 0.0 0.0 0.0 120-121 19.012500000000003 0.0 0.0 0.0 0.0 122-123 19.7625 0.0 0.0 0.0 0.0 124-125 20.775 0.0 0.0 0.0 0.0 126-127 21.5625 0.0 0.0 0.0 0.0 128-129 22.262500000000003 0.0 0.0 0.0 0.0 130-131 23.0875 0.0 0.0 0.0 0.0 132-133 23.825 0.0 0.0 0.0 0.0 134-135 24.6875 0.0 0.0 0.0 0.0 136-137 25.4125 0.0 0.0 0.0 0.0 138-139 26.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATGAC 10 0.006830828 145.0 1 CCCCATG 10 0.006830828 145.0 5 GGGGGGG 575 0.0 10.086956 145 >>END_MODULE Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra Read 1245559 spots for SRR13165359.sra Written 1245559 spots for SRR13165359.sra SRR ids: ['SRR13165359.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_qd3vw7uc SRR13165359.sra spots: 24911180 blocks: [[1, 1245559], [1245560, 2491118], [2491119, 3736677], [3736678, 4982236], [4982237, 6227795], [6227796, 7473354], [7473355, 8718913], [8718914, 9964472], [9964473, 11210031], [11210032, 12455590], [12455591, 13701149], [13701150, 14946708], [14946709, 16192267], [16192268, 17437826], [17437827, 18683385], [18683386, 19928944], [19928945, 21174503], [21174504, 22420062], [22420063, 23665621], [23665622, 24911180]] SRR13165359 file size 8444208 SRR13165359 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165359 SRR13165359_1.fastq SRR13165359_2.fastq Input file: SRR13165359_1.fastq Paired file: SRR13165359_2.fastq trimmed: SRR13165359-trimmed-pair1.fastq, SRR13165359-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:08:08 2024 >> started Sat Dec 7 16:11:19 2024 >> done (190.567s) 24911180 read pairs processed; of these: 559 ( 0.00%) short read pairs filtered out after trimming by size control 142568 ( 0.57%) empty read pairs filtered out after trimming by size control 24768053 (99.43%) read pairs available; of these: 7608759 (30.72%) trimmed read pairs available after processing 17159294 (69.28%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 30 0.00% 19 20 0.00% 20 15 0.00% 21 27 0.00% 22 29 0.00% 23 43 0.00% 24 38 0.00% 25 42 0.00% 26 62 0.00% 27 79 0.00% 28 100 0.00% 29 116 0.00% 30 104 0.00% 31 174 0.00% 32 192 0.00% 33 180 0.00% 34 220 0.00% 35 282 0.00% 36 324 0.00% 37 373 0.00% 38 462 0.00% 39 520 0.00% 40 649 0.00% 41 784 0.00% 42 912 0.00% 43 812 0.00% 44 888 0.00% 45 992 0.00% 46 1194 0.00% 47 1323 0.01% 48 1606 0.01% 49 1910 0.01% 50 2294 0.01% 51 2628 0.01% 52 2921 0.01% 53 3135 0.01% 54 3358 0.01% 55 3471 0.01% 56 4018 0.02% 57 4317 0.02% 58 5261 0.02% 59 5631 0.02% 60 6673 0.03% 61 7654 0.03% 62 8446 0.03% 63 9527 0.04% 64 10476 0.04% 65 10755 0.04% 66 11559 0.05% 67 12736 0.05% 68 14305 0.06% 69 15445 0.06% 70 17524 0.07% 71 19768 0.08% 72 22481 0.09% 73 24685 0.10% 74 27116 0.11% 75 28913 0.12% 76 30384 0.12% 77 32712 0.13% 78 35019 0.14% 79 37377 0.15% 80 40779 0.16% 81 44154 0.18% 82 48949 0.20% 83 52616 0.21% 84 56047 0.23% 85 60200 0.24% 86 63049 0.25% 87 65290 0.26% 88 68145 0.28% 89 70460 0.28% 90 74011 0.30% 91 78110 0.32% 92 81103 0.33% 93 85801 0.35% 94 90756 0.37% 95 93884 0.38% 96 94987 0.38% 97 97739 0.39% 98 97776 0.39% 99 100687 0.41% 100 101398 0.41% 101 102631 0.41% 102 104148 0.42% 103 107147 0.43% 104 109669 0.44% 105 111277 0.45% 106 111902 0.45% 107 113202 0.46% 108 113188 0.46% 109 114190 0.46% 110 113365 0.46% 111 112406 0.45% 112 114309 0.46% 113 113625 0.46% 114 117030 0.47% 115 117910 0.48% 116 117811 0.48% 117 118259 0.48% 118 117019 0.47% 119 117105 0.47% 120 115059 0.46% 121 116539 0.47% 122 115203 0.47% 123 115261 0.47% 124 116909 0.47% 125 116860 0.47% 126 117280 0.47% 127 116293 0.47% 128 115356 0.47% 129 115056 0.46% 130 113437 0.46% 131 112861 0.46% 132 112088 0.45% 133 113083 0.46% 134 111455 0.45% 135 111373 0.45% 136 111849 0.45% 137 111576 0.45% 138 110991 0.45% 139 110681 0.45% 140 109697 0.44% 141 108567 0.44% 142 107702 0.43% 143 107156 0.43% 144 108026 0.44% 145 107709 0.43% 146 106728 0.43% 147 107041 0.43% 148 106999 0.43% 149 107049 0.43% 150 105650 0.43% 151 17159294 69.28% 24768053 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=24.26 fanout-score-rank=5 prefix-density=0.29 prefix-fanout=24.3 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGGCTATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=fanout-score sequence-density=0.06 sequence-density-rank=33 fanout-score=175.82 fanout-score-rank=1 prefix-density=0.57 prefix-fanout=17.8 sequence=GGCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAG criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=6.71 fanout-score-rank=15 prefix-density=0.31 prefix-fanout=4.5 sequence=AAGGAGCTGGAGGAGGTCAAGAAGGAGTA criterion=fanout-score sequence-density=0.12 sequence-density-rank=15 fanout-score=25.37 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=8.5 sequence=GAGAAGCTCCTGTCC SRR13165359 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:15:28 Started mapping on | Dec 07 16:15:38 Finished on | Dec 07 16:32:48 Mapping speed, Million of reads per hour | 86.57 Number of input reads | 24768053 Average input read length | 280 UNIQUE READS: Uniquely mapped reads number | 22916615 Uniquely mapped reads % | 92.52% Average mapped length | 278.63 Number of splices: Total | 17494897 Number of splices: Annotated (sjdb) | 16363688 Number of splices: GT/AG | 17271255 Number of splices: GC/AG | 180033 Number of splices: AT/AC | 10923 Number of splices: Non-canonical | 32686 Mismatch rate per base, % | 0.21% Deletion rate per base | 0.01% Deletion average length | 2.66 Insertion rate per base | 0.01% Insertion average length | 2.19 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 260065 % of reads mapped to multiple loci | 1.05% Number of reads mapped to too many loci | 150943 % of reads mapped to too many loci | 0.61% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.62% % of reads unmapped: other | 2.19% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1591649 1591649 1591649 N_multimapping 260065 260065 260065 N_noFeature 752115 22238901 994205 N_ambiguous 487133 2899 52025 UnstrandedReadsAssigned:21677367 PositiveStrandReadsAssigned:674815 NegativeStrandReadsAssigned:21870385 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=127 echo kmer=123 SRR13165359 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165359-trimmed-pair1.fastq SRR13165359-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,768,053 reads, 22,220,706 reads pseudoaligned [quant] estimated average fragment length: 211.58 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,227 rounds 52973 SRR13165359.ke.tsv 35125 SRR13165359.se.tsv 88098 total ==> SRR13165359.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 725.7 0 0 PNS24247 1044 833.42 27.2681 2.23393 PNS24249 1928 1717.42 87.4466 3.47653 PNS24246 1044 833.42 27.2681 2.23393 PNS24248 1044 833.42 27.2681 2.23393 PNS24244 1471 1260.42 77.7491 4.21172 PNS24243 293 126.337 0 0 KQK14069 1603 1392.42 1.24816 0.0612039 KQK14071 474 276.841 2.75184 0.678693 ==> SRR13165359.se.tsv <== BRADI_1g14170v3 4 BRADI_1g53295v3 14 BRADI_1g59795v3 269 BRADI_1g07683v3 0 BRADI_1g00485v3 85 BRADI_1g20270v3 4967 BRADI_1g74790v3 72 BRADI_1g09890v3 84 BRADI_1g77505v3 158 BRADI_1g48960v3 0 SRR13165359 completed mapping pipeline successfully