Starting /dee2/code/volunteer_pipeline.sh SRR13165360 current disk space = 1542054031360 free memory = 1461975888 SRR13165360 SRAfilesize e371bb0ae982de4ae5f2fcf70b9ae9a7 SRR13165360.sra SRR13165360.sra file validated SRR13165360 is paired end SRR13165360 is conventional basespace SRR13165360 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165360_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.494 37.0 37.0 37.0 37.0 37.0 2 36.28825 37.0 37.0 37.0 37.0 37.0 3 36.4595 37.0 37.0 37.0 37.0 37.0 4 36.511 37.0 37.0 37.0 37.0 37.0 5 36.5575 37.0 37.0 37.0 37.0 37.0 6 36.5785 37.0 37.0 37.0 37.0 37.0 7 36.5085 37.0 37.0 37.0 37.0 37.0 8 36.5445 37.0 37.0 37.0 37.0 37.0 9 36.5505 37.0 37.0 37.0 37.0 37.0 10-14 36.54469999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.4833 37.0 37.0 37.0 37.0 37.0 20-24 36.232099999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.398300000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.3774 37.0 37.0 37.0 37.0 37.0 35-39 36.3423 37.0 37.0 37.0 37.0 37.0 40-44 36.3164 37.0 37.0 37.0 37.0 37.0 45-49 34.97160000000001 37.0 37.0 37.0 29.8 37.0 50-54 35.0951 37.0 37.0 37.0 32.2 37.0 55-59 34.4101 37.0 37.0 37.0 25.0 37.0 60-64 34.514700000000005 37.0 37.0 37.0 27.4 37.0 65-69 34.3605 37.0 37.0 37.0 22.2 37.0 70-74 34.863600000000005 37.0 37.0 37.0 29.8 37.0 75-79 36.028 37.0 37.0 37.0 37.0 37.0 80-84 36.11409999999999 37.0 37.0 37.0 37.0 37.0 85-89 36.1097 37.0 37.0 37.0 37.0 37.0 90-94 36.094199999999994 37.0 37.0 37.0 37.0 37.0 95-99 36.0951 37.0 37.0 37.0 37.0 37.0 100-104 36.0115 37.0 37.0 37.0 37.0 37.0 105-109 36.0602 37.0 37.0 37.0 37.0 37.0 110-114 35.9644 37.0 37.0 37.0 37.0 37.0 115-119 35.923899999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.7804 37.0 37.0 37.0 37.0 37.0 125-129 35.576 37.0 37.0 37.0 37.0 37.0 130-134 35.406400000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.1783 37.0 37.0 37.0 34.6 37.0 140-144 34.893 37.0 37.0 37.0 27.4 37.0 145-149 34.4962 37.0 37.0 37.0 25.0 37.0 150-151 34.042500000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 1.0 23 2.0 24 5.0 25 4.0 26 9.0 27 12.0 28 16.0 29 32.0 30 46.0 31 56.0 32 182.0 33 355.0 34 178.0 35 381.0 36 2369.0 37 350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.9 9.55 6.275 34.275 2 21.27606129113288 15.87540818889726 27.98291886460688 34.86561165536297 3 17.675 10.025 33.85 38.45 4 24.075 15.4 19.900000000000002 40.625 5 33.575 20.849999999999998 21.15 24.425 6 33.525 22.85 20.200000000000003 23.425 7 19.225 26.75 33.95 20.075000000000003 8 17.925 27.025 28.799999999999997 26.25 9 30.349999999999998 17.125 27.775 24.75 10-14 24.11 23.375 22.345000000000002 30.17 15-19 24.375 20.849999999999998 24.654999999999998 30.12 20-24 25.16 22.925 24.9 27.015 25-29 24.955 20.595 24.52 29.93 30-34 22.650000000000002 20.215 26.325 30.81 35-39 23.66 22.735 24.175 29.43 40-44 26.82 19.665 24.474999999999998 29.04 45-49 24.51 19.869999999999997 26.33 29.29 50-54 25.290000000000003 18.955 25.314999999999998 30.44 55-59 22.075 19.46 28.110000000000003 30.354999999999997 60-64 25.72 18.75 27.694999999999997 27.834999999999997 65-69 24.6 24.535 24.055 26.810000000000002 70-74 31.605 18.509999999999998 22.045 27.839999999999996 75-79 31.424999999999997 19.78 21.915000000000003 26.88 80-84 31.435000000000002 20.07 22.07 26.424999999999997 85-89 31.78 19.245 21.735 27.24 90-94 31.04 20.044999999999998 21.555 27.36 95-99 30.349999999999998 20.525 21.33 27.794999999999998 100-104 31.514999999999997 20.565 21.575 26.345000000000002 105-109 31.935000000000002 20.395 21.43 26.240000000000002 110-114 31.619999999999997 19.939999999999998 21.82 26.619999999999997 115-119 31.924999999999997 20.65 20.935000000000002 26.490000000000002 120-124 32.269999999999996 21.735 20.765 25.230000000000004 125-129 31.395 20.985 20.419999999999998 27.200000000000003 130-134 32.1 20.22 21.09 26.590000000000003 135-139 32.095 20.7 21.21 25.995 140-144 32.12 20.715 20.880000000000003 26.284999999999997 145-149 32.795 20.445 21.07 25.69 150-151 32.675 20.5125 21.3875 25.424999999999997 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 1.0 28 1.0 29 0.0 30 0.0 31 0.0 32 1.0 33 1.5 34 2.5 35 5.0 36 6.5 37 10.0 38 11.5 39 12.5 40 16.5 41 24.5 42 29.0 43 33.0 44 40.5 45 57.5 46 78.5 47 102.0 48 112.0 49 136.5 50 181.5 51 184.0 52 190.0 53 212.0 54 229.5 55 284.5 56 319.5 57 275.0 58 222.0 59 171.0 60 110.5 61 89.5 62 80.5 63 80.5 64 82.0 65 83.5 66 101.0 67 104.5 68 83.5 69 53.0 70 40.0 71 28.0 72 23.5 73 23.5 74 14.0 75 11.0 76 11.0 77 8.0 78 9.5 79 6.0 80 0.5 81 1.0 82 1.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.5 #Duplication Level Percentage of deduplicated Percentage of total 1 61.067961165048544 31.45 2 20.631067961165048 21.25 3 7.766990291262135 12.0 4 4.320388349514563 8.9 5 2.7184466019417477 7.000000000000001 6 1.5048543689320388 4.65 7 0.7766990291262136 2.8000000000000003 8 0.1941747572815534 0.8 9 0.2912621359223301 1.35 >10 0.6796116504854369 6.9 >50 0.0 0.0 >100 0.04854368932038835 2.9000000000000004 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCTCGTAT 116 2.9000000000000004 TruSeq Adapter, Index 19 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCTCGTTT 50 1.25 TruSeq Adapter, Index 19 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCTCGGAT 47 1.175 TruSeq Adapter, Index 19 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCGCGTAT 30 0.75 TruSeq Adapter, Index 19 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCTCGGTT 25 0.625 TruSeq Adapter, Index 19 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCGCGTTT 20 0.5 TruSeq Adapter, Index 19 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCGCGGAT 17 0.42500000000000004 TruSeq Adapter, Index 19 (97% over 37bp) GTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGCCT 13 0.325 No Hit GTTCGCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCAGTTTAAA 12 0.3 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 11 0.27499999999999997 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 11 0.27499999999999997 No Hit GCCCAGATGGCCGGGTGTGGGTCGCGCGCTTTAGCGCCATCCATTTTCGG 10 0.25 No Hit CCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAG 10 0.25 No Hit GCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTC 10 0.25 No Hit CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCT 10 0.25 No Hit GTTAGCTACAGCACTGCACGGGTCGAGTCGCACAGCACCTAGTATCCATC 9 0.22499999999999998 No Hit CGTGGGTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATT 9 0.22499999999999998 No Hit GCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCC 9 0.22499999999999998 No Hit CTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGTAGCGCCTGGTATG 9 0.22499999999999998 No Hit GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTA 9 0.22499999999999998 No Hit GGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCG 9 0.22499999999999998 No Hit GTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATT 8 0.2 No Hit GTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTAC 8 0.2 No Hit GTCGGTTCGGACCTCTGCTTAGTTTCATCCAAGCTTCATCCTGGTCATGG 8 0.2 No Hit GCCGATGCTTATTCCTCAGATACCGTCATTGTTTCTTCTCCGAGAAAAGA 8 0.2 No Hit GCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGT 7 0.17500000000000002 No Hit CCCTAATTCTCCGTCACCCGTCACCACCATGGTAGGCCCCTATCCTACCA 7 0.17500000000000002 No Hit ATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAG 7 0.17500000000000002 No Hit GGCGATAATAAATACAGAAGTTGCGGTCAATAAGGTAGGGATCATCAAAA 7 0.17500000000000002 No Hit GCCGACTTTCGTCTCTGCTCGACGGGTGAGTCTTGCAGTCAAGCTCCCTT 7 0.17500000000000002 No Hit CTCATCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCT 7 0.17500000000000002 No Hit ACCTAGTATCCATCGTTTACGGCTAGGACTACTGGGGTCTCTAATCCCAT 7 0.17500000000000002 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 7 0.17500000000000002 No Hit GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATC 7 0.17500000000000002 No Hit ATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCTGGCACAGA 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCGCGGTT 7 0.17500000000000002 TruSeq Adapter, Index 19 (97% over 37bp) GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCG 7 0.17500000000000002 No Hit CGTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACA 7 0.17500000000000002 No Hit GCCCAATCATTCCGGATAACGCTTGCATCCTCTGTCTTACCGCGGCTGCT 7 0.17500000000000002 No Hit CCGTCATTGTTTCTTCTCCGAGAAAAGAAGTTGACGACCCGTAGGCCTTC 7 0.17500000000000002 No Hit GGTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTG 7 0.17500000000000002 No Hit CTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAAGACTTT 6 0.15 No Hit CTCCAGGTGGCGAAGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATG 6 0.15 No Hit ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC 6 0.15 No Hit GTCGGATGGGGAGCCCGCAGGCCGTTGCAGCGCAGTGCCCCGAGGGACAC 6 0.15 No Hit GGATAGATCACCCAGGTTCGGGTCCATAAGCAGTGACAATCGCCCTATGA 6 0.15 No Hit GTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGCCGT 6 0.15 No Hit GTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCAT 6 0.15 No Hit GCACTCTTTAAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGCTGTCTTTG 6 0.15 No Hit GTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGG 6 0.15 No Hit CCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTC 6 0.15 No Hit AGCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCT 6 0.15 No Hit GGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTAC 6 0.15 No Hit GGGGTAGTACAGGAATATTGACCTGTTGTCCATCGACTACGCCTTTCGGC 6 0.15 No Hit GCTAATCAGACGCGAGCCCCTCCTTGGGCGGATTTCTCCTTTTGCTCCTC 6 0.15 No Hit GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA 6 0.15 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 6 0.15 No Hit GTTCGCTGGAGTAGGCTGCGAGGCCCAAGCGGCCAAGGAGTGCAGCGGCC 6 0.15 No Hit CGGCAACGAGTTTGAGACAGATGTTAAGGCTATGCTTCTGAGGGCTAACC 6 0.15 No Hit CCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAA 6 0.15 No Hit CCCCTAACCACAACTCATCCGCTGATTCTTCAACATCAGTCGGTTCGGAC 6 0.15 No Hit GTGGGTCGGGGCAGGCGGCGGGCGCAGGCGCCGCTTGCTAGCTTGGATTC 6 0.15 No Hit CTCTTACTCATGTCCGGGACGCGGTCGGCGGAGATGCTGCCGGCCATGCA 6 0.15 No Hit GTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT 6 0.15 No Hit GGCAACTAAACACGAGGGTTGCGCTCGTTGCGAGACTTAACCCAACACCT 6 0.15 No Hit GATCGGAAGAGCACACGTCGGAACTCCAGTCACTTGACAGGATCTCGTAT 6 0.15 TruSeq Adapter, Index 3 (97% over 35bp) CCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGG 6 0.15 No Hit TGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCT 6 0.15 No Hit GCCTCATCCTCTCCTTCCTCCGGCTTAACACCGGCGGTCTGTTCAGGGTT 6 0.15 No Hit GCTCCCTTCTGCCTTTGCACTCGAGGACCAATGTCCGTCTGGCCCGAGGA 6 0.15 No Hit CTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGG 6 0.15 No Hit GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT 6 0.15 No Hit CCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCG 5 0.125 No Hit GCCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCT 5 0.125 No Hit GCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTA 5 0.125 No Hit GCGGCACGGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAA 5 0.125 No Hit TTCTTGGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCG 5 0.125 No Hit CCTTGGCCCGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGT 5 0.125 No Hit GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGT 5 0.125 No Hit GCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTCG 5 0.125 No Hit GTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCT 5 0.125 No Hit GCTTTCAGTGGGTTCAATCATAAGTGTGCCTGGAACAGGCCATGACATGG 5 0.125 No Hit CACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTCCAGCTTGGTA 5 0.125 No Hit CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC 5 0.125 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 5 0.125 No Hit GTGGTATTTCACTTGCGCCCGTAAAGGCTCCCACTTATCCTACACCTCTC 5 0.125 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 5 0.125 No Hit CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCT 5 0.125 No Hit GGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTT 5 0.125 No Hit AACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGG 5 0.125 No Hit GCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCAGCGCTCGGGGAAAG 5 0.125 No Hit CTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAA 5 0.125 No Hit ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATT 5 0.125 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 5 0.125 No Hit CCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAATATTGACCTGTT 5 0.125 No Hit CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT 5 0.125 No Hit CTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAG 5 0.125 No Hit GGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTT 5 0.125 No Hit GCCCCATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCGCGGGTCTGA 5 0.125 No Hit GTCCGTCTGGCCCGAGGAAACCTTTGCACGCCTCCGTTACCTTTTGGGAG 5 0.125 No Hit GCCGCTACTACGGGAATCGCTTTTGCTTTCTTTTCCTCTGGCTACTAAGA 5 0.125 No Hit TTACAATGCCCCGTCGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGC 5 0.125 No Hit GGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCA 5 0.125 No Hit GGCAGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTC 5 0.125 No Hit ATTCAGAGTTTGCCTCGATTTGGTACCGCTCGCGCAGCCCGCACCGAAAC 5 0.125 No Hit CCCGTAAAGGCTCCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGT 5 0.125 No Hit GGCAGTTTAAAAGGTTGACCTATTTGGGAATCTCCGGATCTATGCTTATT 5 0.125 No Hit GCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT 5 0.125 No Hit GTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATT 5 0.125 No Hit GCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGC 5 0.125 No Hit GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA 5 0.125 No Hit GTGACGGGCGGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGC 5 0.125 No Hit CCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTA 5 0.125 No Hit CGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGG 5 0.125 No Hit CGAGAAAAGAAGTTGACGACCCGTAGGCCTTCCACCTCCACGCGGCATTG 5 0.125 No Hit GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT 5 0.125 No Hit GGGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATG 5 0.125 No Hit CCCGTCCCTTAGGATCGGCTTACCCATGTGCAAGTGCCGTTCACATGGAA 5 0.125 No Hit GCCGTTGGTGTTCTTTCCGATCTCAATGCATTTCACCGCTCCACCGGAAA 5 0.125 No Hit CTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCC 5 0.125 No Hit CTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT 5 0.125 No Hit CTCATACTTCAGCGCTGCAGCGCTTGGTACTCGGACCTCGTCTCGAGGCA 5 0.125 No Hit CCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGAC 5 0.125 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 5 0.125 No Hit CCCGGATCGGCCCGGTCAGACCGGGCCTTGGAGCCAAAAGGAGGGGACTT 5 0.125 No Hit GCTCCCCTACCGATGCATTTTGACATCCCACAGCTTCGGCAGATCGCTTA 5 0.125 No Hit ACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTC 5 0.125 No Hit CCCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0125 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.037500000000000006 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.0625 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.175 0.0 0.0 0.0 0.0 60-61 0.2375 0.0 0.0 0.0 0.0 62-63 0.275 0.0 0.0 0.0 0.0 64-65 0.35 0.0 0.0 0.0 0.0 66-67 0.425 0.0 0.0 0.0 0.0 68-69 0.475 0.0 0.0 0.0 0.0 70-71 0.5375000000000001 0.0 0.0 0.0 0.0 72-73 0.7125 0.0 0.0 0.0 0.0 74-75 0.8374999999999999 0.0 0.0 0.0 0.0 76-77 1.15 0.0 0.0 0.0 0.0 78-79 1.4625 0.0 0.0 0.0 0.0 80-81 1.975 0.0 0.0 0.0 0.0 82-83 2.4125 0.0 0.0 0.0 0.0 84-85 2.9375 0.0 0.0 0.0 0.0 86-87 3.45 0.0 0.0 0.0 0.0 88-89 3.8875 0.0 0.0 0.0 0.0 90-91 4.6 0.0 0.0 0.0 0.0 92-93 5.199999999999999 0.0 0.0 0.0 0.0 94-95 6.1625 0.0 0.0 0.0 0.0 96-97 6.975 0.0 0.0 0.0 0.0 98-99 7.6 0.0 0.0 0.0 0.0 100-101 8.5125 0.0 0.0 0.0 0.0 102-103 9.5 0.0 0.0 0.0 0.0 104-105 10.287500000000001 0.0 0.0 0.0 0.0 106-107 11.4875 0.0 0.0 0.0 0.0 108-109 12.725000000000001 0.0 0.0 0.0 0.0 110-111 13.9 0.0 0.0 0.0 0.0 112-113 14.7625 0.0 0.0 0.0 0.0 114-115 15.9625 0.0 0.0 0.0 0.0 116-117 17.0625 0.0 0.0 0.0 0.0 118-119 18.15 0.0 0.0 0.0 0.0 120-121 19.275 0.0 0.0 0.0 0.0 122-123 20.225 0.0 0.0 0.0 0.0 124-125 21.2 0.0 0.0 0.0 0.0 126-127 22.1875 0.0 0.0 0.0 0.0 128-129 23.35 0.0 0.0 0.0 0.0 130-131 24.325 0.0 0.0 0.0 0.0 132-133 25.5125 0.0 0.0 0.0 0.0 134-135 26.85 0.0 0.0 0.0 0.0 136-137 27.8375 0.0 0.0 0.0 0.0 138-139 28.9 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGAGCA 165 3.8658982E-8 35.151516 7 GAGCACA 165 3.8658982E-8 35.151516 9 AGAGCAC 165 3.8658982E-8 35.151516 8 GAAGAGC 170 5.0296876E-8 34.11765 6 CGGAAGA 175 6.4930646E-8 33.142857 4 GGAAGAG 175 6.4930646E-8 33.142857 5 GATCGGA 185 1.0589247E-7 31.351349 1 TCGGAAG 185 1.0589247E-7 31.351349 3 ATCGGAA 185 1.0589247E-7 31.351349 2 AAGGGGG 25 4.977651E-4 29.0 65-69 AAAAGGG 20 0.00593511 29.0 65-69 AAAGGGG 25 4.977651E-4 29.0 65-69 TGAAAAG 20 0.00593511 29.0 60-64 AGGGGGG 40 0.0076550315 18.125 65-69 CAGTCAC 120 0.0036335043 18.125 145 TGCCGTC 40 0.0076550315 18.125 50-54 CCGTCTT 40 0.0076550315 18.125 50-54 GCCGTCT 40 0.0076550315 18.125 50-54 GATCTCG 60 0.004491891 14.500001 40-44 AGGATCT 65 0.0076375785 13.384615 35-39 >>END_MODULE SRR13165360 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165360_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.06975 37.0 37.0 37.0 37.0 37.0 2 36.199 37.0 37.0 37.0 37.0 37.0 3 35.9815 37.0 37.0 37.0 37.0 37.0 4 35.9295 37.0 37.0 37.0 37.0 37.0 5 36.0535 37.0 37.0 37.0 37.0 37.0 6 36.1015 37.0 37.0 37.0 37.0 37.0 7 35.838 37.0 37.0 37.0 37.0 37.0 8 35.3895 37.0 37.0 37.0 37.0 37.0 9 35.316 37.0 37.0 37.0 37.0 37.0 10-14 35.1586 37.0 37.0 37.0 29.8 37.0 15-19 35.08710000000001 37.0 37.0 37.0 27.4 37.0 20-24 35.000099999999996 37.0 37.0 37.0 27.4 37.0 25-29 34.2505 37.0 37.0 37.0 25.0 37.0 30-34 34.1524 37.0 37.0 37.0 22.2 37.0 35-39 33.967 37.0 37.0 37.0 16.6 37.0 40-44 34.1822 37.0 37.0 37.0 25.0 37.0 45-49 33.924850000000006 37.0 37.0 37.0 11.0 37.0 50-54 34.009750000000004 37.0 37.0 37.0 19.4 37.0 55-59 34.3245 37.0 37.0 37.0 25.0 37.0 60-64 34.6711 37.0 37.0 37.0 25.0 37.0 65-69 34.286199999999994 37.0 37.0 37.0 25.0 37.0 70-74 33.853899999999996 37.0 37.0 37.0 16.6 37.0 75-79 33.85865 37.0 37.0 37.0 16.6 37.0 80-84 34.50155 37.0 37.0 37.0 25.0 37.0 85-89 35.1321 37.0 37.0 37.0 32.2 37.0 90-94 35.30505 37.0 37.0 37.0 37.0 37.0 95-99 35.561699999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.5329 37.0 37.0 37.0 37.0 37.0 105-109 35.5246 37.0 37.0 37.0 37.0 37.0 110-114 35.453199999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.424099999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.146100000000004 37.0 37.0 37.0 34.6 37.0 125-129 35.05135 37.0 37.0 37.0 34.6 37.0 130-134 34.7789 37.0 37.0 37.0 25.0 37.0 135-139 34.609500000000004 37.0 37.0 37.0 25.0 37.0 140-144 34.2633 37.0 37.0 37.0 25.0 37.0 145-149 33.9878 37.0 37.0 37.0 25.0 37.0 150-151 33.441500000000005 37.0 37.0 37.0 18.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 11.0 14 15.0 15 9.0 16 4.0 17 6.0 18 9.0 19 6.0 20 17.0 21 14.0 22 25.0 23 34.0 24 38.0 25 43.0 26 55.0 27 78.0 28 68.0 29 32.0 30 20.0 31 43.0 32 75.0 33 124.0 34 221.0 35 483.0 36 2347.0 37 220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.100677880994226 15.89254330906352 7.331157419030881 23.675621390911374 2 39.65 21.075 21.525 17.75 3 38.375 19.075 22.225 20.325 4 36.925000000000004 29.525000000000002 15.174999999999999 18.375 5 38.0 28.525 15.9 17.575 6 33.525 31.874999999999996 15.625 18.975 7 33.074999999999996 18.6 26.8 21.525 8 34.575 19.5 21.224999999999998 24.7 9 35.125 21.775 20.775 22.325 10-14 35.535 24.26 18.145 22.06 15-19 36.69 23.369999999999997 19.055 20.885 20-24 35.91795897948975 23.84192096048024 18.60430215107554 21.635817908954476 25-29 35.918878317476214 22.70405608412619 18.893340010015024 22.483725588382576 30-34 34.13071764588129 23.621259133219898 21.008908017215493 21.239115203683316 35-39 32.311155577788895 25.287643821910955 21.200600300150075 21.200600300150075 40-44 34.55955955955956 23.85885885885886 20.025025025025027 21.556556556556554 45-49 31.46860145108832 23.512634475856892 22.61195896922692 22.40680510382787 50-54 33.90525736581462 23.965784603071384 20.664298934520534 21.464659096593465 55-59 34.59689534301452 23.00951427140711 20.771156735102654 21.622433650475713 60-64 35.907953976988495 22.681340670335167 19.054527263631815 22.356178089044523 65-69 35.7121424284857 23.36967393478696 18.76375275055011 22.154430886177234 70-74 34.481722583875815 23.85578367551327 19.464196294441663 22.198297446169253 75-79 33.840532559187146 23.409580059062016 20.53656339156114 22.2133239901897 80-84 35.02875718929733 23.335833958489623 19.419854963740935 22.215553888472115 85-89 35.43106037849204 22.62941824371683 18.809452287974366 23.130069089816764 90-94 36.58512181699935 23.808094451948573 18.85036770223623 20.75641602881585 95-99 37.15986394557823 22.759103641456583 18.262304921968788 21.818727490996398 100-104 36.88032048072108 24.58187280921382 17.95192789183776 20.58587881822734 105-109 37.702783897456435 23.382735830162225 18.44582415381534 20.468656118565992 110-114 37.51375687843922 24.627313656828413 18.28914457228614 19.569784892446222 115-119 37.93932111745269 23.665765495143688 18.15359967958346 20.241313707820165 120-124 39.16374561842764 23.84576865297947 17.781672508763144 19.208813219829747 125-129 38.753441802252816 23.339173967459324 18.07759699624531 19.829787234042552 130-134 38.89834752128192 22.42363545317977 18.377566349524287 20.30045067601402 135-139 40.30545818728093 22.844266399599398 18.5678517776665 18.282423635453178 140-144 41.76088044022011 22.141070535267634 18.659329664832416 17.43871935967984 145-149 41.517275913870805 22.158237356034054 18.202303455182776 18.122183274912366 150-151 43.56534802203305 22.69654481722584 17.676514772158235 16.061592388582874 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 1.0 9 1.5 10 0.5 11 1.0 12 1.0 13 0.5 14 0.5 15 0.5 16 1.5 17 2.0 18 1.5 19 1.5 20 2.0 21 1.0 22 1.0 23 3.0 24 3.0 25 1.0 26 1.5 27 2.5 28 2.5 29 2.0 30 1.5 31 3.0 32 6.0 33 7.5 34 7.0 35 9.5 36 10.5 37 13.0 38 14.5 39 24.5 40 30.5 41 34.5 42 40.5 43 50.5 44 62.0 45 70.0 46 79.0 47 114.0 48 129.0 49 126.5 50 150.0 51 160.0 52 157.5 53 190.5 54 264.5 55 274.0 56 260.5 57 228.0 58 161.0 59 158.0 60 139.0 61 94.5 62 90.0 63 79.5 64 61.0 65 42.5 66 31.0 67 37.0 68 45.5 69 41.0 70 30.0 71 18.5 72 14.0 73 14.5 74 13.0 75 12.5 76 17.0 77 13.5 78 7.0 79 8.0 80 6.0 81 3.0 82 3.5 83 6.5 84 7.0 85 6.0 86 6.5 87 12.0 88 20.0 89 21.5 90 18.0 91 19.5 92 22.5 93 24.5 94 25.0 95 24.0 96 21.5 97 20.0 98 19.0 99 12.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.15 30-34 0.09 35-39 0.05 40-44 0.1 45-49 0.075 50-54 0.045 55-59 0.15 60-64 0.05 65-69 0.02 70-74 0.15 75-79 0.105 80-84 0.025 85-89 0.13 90-94 0.055 95-99 0.04 100-104 0.15 105-109 0.13999999999999999 110-114 0.05 115-119 0.13 120-124 0.15 125-129 0.125 130-134 0.15 135-139 0.15 140-144 0.05 145-149 0.15 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.724999999999994 #Duplication Level Percentage of deduplicated Percentage of total 1 66.72247802427795 39.85 2 18.334030975303474 21.9 3 7.157806613645877 12.825000000000001 4 3.1393888656341566 7.5 5 1.9673503557974048 5.875 6 1.1720385098367516 4.2 7 0.6278777731268314 2.625 8 0.3348681456676434 1.6 9 0.2092925910422771 1.125 >10 0.3348681456676434 2.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 24 0.6 No Hit GTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCGTGTTTAGTTGCCACTA 13 0.325 No Hit CGGACATTGGTCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGAC 12 0.3 No Hit CCTTACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCC 11 0.27499999999999997 No Hit GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC 10 0.25 No Hit GGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGG 10 0.25 No Hit CAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAA 10 0.25 No Hit ATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAA 10 0.25 No Hit GTGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTT 9 0.22499999999999998 No Hit GTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAACCTAGT 9 0.22499999999999998 No Hit GCCGATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATCACGCGTA 9 0.22499999999999998 No Hit CGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACTGG 9 0.22499999999999998 No Hit GTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCCTCAGTTCG 9 0.22499999999999998 No Hit GGGGCGCTTGCGCCCCCAAGGGCACGTGCCATTGGCTAAGCCGTTCCGGC 8 0.2 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCC 8 0.2 No Hit AGCTTACCAAGGCGATGATCAGTAGCTGGTCCGAGAGGATGATCAGCCAC 8 0.2 No Hit TAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGGAGGGG 8 0.2 No Hit GAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAA 8 0.2 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 8 0.2 No Hit GGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATAGATC 8 0.2 No Hit AGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGC 8 0.2 No Hit CTGAACGCCTCTAAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGCGCCCG 7 0.17500000000000002 No Hit GTGAAAAGAACCCCCAGTGGGTAGTGAAATAGAACGTGAAACCGTGCTGA 7 0.17500000000000002 No Hit GGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAA 7 0.17500000000000002 No Hit GGAGATTCCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAG 7 0.17500000000000002 No Hit GTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTAA 7 0.17500000000000002 No Hit GGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCA 7 0.17500000000000002 No Hit GTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAA 7 0.17500000000000002 No Hit GTTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAA 7 0.17500000000000002 No Hit AGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGA 7 0.17500000000000002 No Hit CGTATGCGGAACGGCTTTTGCTGGTCCGCCGCTCGGCTCGGGGCGTGGAC 7 0.17500000000000002 No Hit GGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCG 7 0.17500000000000002 No Hit CCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACACCAACGGCGAA 7 0.17500000000000002 No Hit GCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCG 7 0.17500000000000002 No Hit CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGAGGATGAGGCCAA 7 0.17500000000000002 No Hit GGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGC 7 0.17500000000000002 No Hit CCGAATCCTCTCTATGGGCCAAGCACCCTTTGTTGCCAAAAGAAAAGCTA 6 0.15 No Hit CCTGGACAGAAAGACCCTATGAAGCTTTACTGTTCCCTGGGATTGGCTTT 6 0.15 No Hit GGCTGATCTTCCCCAAGAGTCCACATCGACGGGAAGGTTTGGCACCTCGA 6 0.15 No Hit CGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTGCCGTAAGGTGTTGG 6 0.15 No Hit CCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAAAGTGATCTCTGACCGCGT 6 0.15 No Hit GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAA 6 0.15 No Hit CAAAAGGGTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACGAACC 6 0.15 No Hit CCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTG 6 0.15 No Hit GGGAGTTGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTT 6 0.15 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 6 0.15 No Hit TACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGA 6 0.15 No Hit TGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGGAAGTGGTGT 6 0.15 No Hit GAGCTCCACCTTGCACCTAGCCATGGCTTCCACCGCGCTCTCCACCGCCT 6 0.15 No Hit CTGACACTGAGAGACGAAAGCTAGGGGAGCAAATGGGATTAGAGACCCCA 6 0.15 No Hit TGTACCCGAAACCGACACAGGTGGGTAGGTAGAGAATACCTAGGGGCGCG 6 0.15 No Hit CTTGGTTAAGGGAACGGAACCCACCGGAGCCGTAGCGAAAGCGAGTCTTC 6 0.15 No Hit GTGGGAACTTAGTTTCCGTTTGGGTATGCGCCCTTGGATTGCTGTTGCAT 6 0.15 No Hit TCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGGAGGGGCGCATTT 6 0.15 No Hit GACAAAGGGTCGCGATCTCGCGAGGGTGAGCTAACTCCAAAAACCCGTCC 6 0.15 No Hit GGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATCCGCCCAAG 6 0.15 No Hit GTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTCAACG 6 0.15 No Hit CACACGTGCTACAATGGGCGGGACAAAGGGTCGCGATCTCGCGAGGGTGA 6 0.15 No Hit GGAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGA 6 0.15 No Hit GGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCC 6 0.15 No Hit GTCGTGCCTCCGGCATCGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAA 6 0.15 No Hit CGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGCTAAA 6 0.15 No Hit GCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACCCTCG 6 0.15 No Hit ATCCAATGCCCCGAAAACCCAAGGTTTCCTCCGCAAGGTTCGTCCACGGA 6 0.15 No Hit GGGAAAGTGATCTCTGACCGCGTGCCTGTTGAAGAATGAGCCGGCGACTC 5 0.125 No Hit AAGAACACCAACGGCGAAAGCACTCTGCTGGGCCGACACTGACACTGAGA 5 0.125 No Hit CAAACCCCGACTTCTGGGAGGGGCGCATTTATTAGATAAAAGGCTGACGC 5 0.125 No Hit GCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAAC 5 0.125 No Hit GGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGC 5 0.125 No Hit GACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTA 5 0.125 No Hit CTCTTCGCCACCTGGAGCTGTAGGTGGTTCCAAGGGTTGGGCTGTTCGCC 5 0.125 No Hit GAAGCTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTT 5 0.125 No Hit ATGGATACTAGGTGCTGTGCGACTCGACCCGTGCAGTGCTGTAGCTAACG 5 0.125 No Hit TTCCCTAGTACGAGAGGACCGGGAAGGACGCACCTCTGGTGTACCAGTTA 5 0.125 No Hit CGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGG 5 0.125 No Hit AACCGTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGG 5 0.125 No Hit GCCACCCTTTAAAGAGTGCGTAATAGCTCACTGATCGAGCGCCCTTGCGC 5 0.125 No Hit ACTACCCCTTGTTGGTACGGAGGGACGGAGGAGGCTAGGTTAGCCGAAAG 5 0.125 No Hit CGAACCGTGAAAGCGTGGCCTATCGATCCTTTAGATCTTCGGAGTTTGAA 5 0.125 No Hit TGAAACTAAGCAGAGGTCCGAACCGACTGATGTTGAAGAATCAGCGGATG 5 0.125 No Hit GTCGAACGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAA 5 0.125 No Hit GCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTTGAGGCGC 5 0.125 No Hit CTTGCAATTTGAATAAACCAAGATCTTACCATGACTGCAATTTTAGAGAG 5 0.125 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 5 0.125 No Hit AGAAGGCCCCCTTCCGGGGGGGCCCGAGCCATCAGTGAGATACCACTCTG 5 0.125 No Hit GACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTG 5 0.125 No Hit GAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCC 5 0.125 No Hit GTGGAAGGCCTACGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGA 5 0.125 No Hit GGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCA 5 0.125 No Hit TGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCCCA 5 0.125 No Hit GAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTA 5 0.125 No Hit GGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTC 5 0.125 No Hit GTTGGCCTTCGGGATCGGAGTAATGATTAATAGGGACAGTCGGGGGCATT 5 0.125 No Hit CATTATTGACTTAAGAGGGTTTAAGGCAACTGCTGGTATAGAGCCTGAGG 5 0.125 No Hit GTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACC 5 0.125 No Hit AACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTAAAG 5 0.125 No Hit GGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGC 5 0.125 No Hit GGAGAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCA 5 0.125 No Hit CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGC 5 0.125 No Hit GGGACGGAGGAGGCTAGGTTAGCCGAAAGATGGTTATAGGTTTAAGGACA 5 0.125 No Hit GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA 5 0.125 No Hit GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAG 5 0.125 No Hit AATGGATTAACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCA 5 0.125 No Hit GTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGGGTCGGAA 5 0.125 No Hit GTGAAATAGAACGTGAAACCGTGCTGAGCTCCCAAGCAGTGGGAGGGGAA 5 0.125 No Hit CCCAGCTTGAGAATCGGGCGGCCGTGCCGTCCGAATTGTAGTCTGGAGAG 5 0.125 No Hit TGATTAATAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAA 5 0.125 No Hit CTCATAGGCAGTGGCTTGGTTAAGGGAACGGAACCCACCGGAGCCGTAGC 5 0.125 No Hit GGGATGTCAAAATGCATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAGCA 5 0.125 No Hit CGGGTCGTCAACTTCTTTTCTCGGAGAAGAAACAATGACGGTATCTGAGG 5 0.125 No Hit GTCAGCTCGTGCCGTAAGGTGTTGGGTTAAGTCTCGCAACGAGCGCAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0125 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.037500000000000006 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.0625 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.175 0.0 0.0 0.0 0.0 60-61 0.2375 0.0 0.0 0.0 0.0 62-63 0.2625 0.0 0.0 0.0 0.0 64-65 0.3 0.0 0.0 0.0 0.0 66-67 0.35 0.0 0.0 0.0 0.0 68-69 0.4 0.0 0.0 0.0 0.0 70-71 0.4625 0.0 0.0 0.0 0.0 72-73 0.6375 0.0 0.0 0.0 0.0 74-75 0.7625 0.0 0.0 0.0 0.0 76-77 1.075 0.0 0.0 0.0 0.0 78-79 1.3875000000000002 0.0 0.0 0.0 0.0 80-81 1.9125 0.0 0.0 0.0 0.0 82-83 2.3625 0.0 0.0 0.0 0.0 84-85 2.8875 0.0 0.0 0.0 0.0 86-87 3.4000000000000004 0.0 0.0 0.0 0.0 88-89 3.8375 0.0 0.0 0.0 0.0 90-91 4.55 0.0 0.0 0.0 0.0 92-93 5.1375 0.0 0.0 0.0 0.0 94-95 6.1125 0.0 0.0 0.0 0.0 96-97 6.925 0.0 0.0 0.0 0.0 98-99 7.6 0.0 0.0 0.0 0.0 100-101 8.537500000000001 0.0 0.0 0.0 0.0 102-103 9.55 0.0 0.0 0.0 0.0 104-105 10.35 0.0 0.0 0.0 0.0 106-107 11.5875 0.0 0.0 0.0 0.0 108-109 12.8375 0.0 0.0 0.0 0.0 110-111 14.025 0.0 0.0 0.0 0.0 112-113 14.899999999999999 0.0 0.0 0.0 0.0 114-115 16.0875 0.0 0.0 0.0 0.0 116-117 17.200000000000003 0.0 0.0 0.0 0.0 118-119 18.3 0.0 0.0 0.0 0.0 120-121 19.4 0.0 0.0 0.0 0.0 122-123 20.35 0.0 0.0 0.0 0.0 124-125 21.325 0.0 0.0 0.0 0.0 126-127 22.3125 0.0 0.0 0.0 0.0 128-129 23.45 0.0 0.0 0.0 0.0 130-131 24.45 0.0 0.0 0.0 0.0 132-133 25.6625 0.0 0.0 0.0 0.0 134-135 27.0 0.0 0.0 0.0 0.0 136-137 28.012500000000003 0.0 0.0 0.0 0.0 138-139 29.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCCAG 10 0.006830828 145.0 6 GGGGGGT 75 0.0012377208 13.533334 25-29 >>END_MODULE Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157257 spots for SRR13165360.sra Written 1157257 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra Read 1157254 spots for SRR13165360.sra Written 1157254 spots for SRR13165360.sra SRR ids: ['SRR13165360.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_o1l5zvoq SRR13165360.sra spots: 23145083 blocks: [[1, 1157254], [1157255, 2314508], [2314509, 3471762], [3471763, 4629016], [4629017, 5786270], [5786271, 6943524], [6943525, 8100778], [8100779, 9258032], [9258033, 10415286], [10415287, 11572540], [11572541, 12729794], [12729795, 13887048], [13887049, 15044302], [15044303, 16201556], [16201557, 17358810], [17358811, 18516064], [18516065, 19673318], [19673319, 20830572], [20830573, 21987826], [21987827, 23145083]] SRR13165360 file size 7844011 SRR13165360 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165360 SRR13165360_1.fastq SRR13165360_2.fastq Input file: SRR13165360_1.fastq Paired file: SRR13165360_2.fastq trimmed: SRR13165360-trimmed-pair1.fastq, SRR13165360-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:07:59 2024 >> started Sat Dec 7 16:11:22 2024 >> done (203.276s) 23145083 read pairs processed; of these: 1059 ( 0.00%) short read pairs filtered out after trimming by size control 1759014 ( 7.60%) empty read pairs filtered out after trimming by size control 21385010 (92.40%) read pairs available; of these: 7722162 (36.11%) trimmed read pairs available after processing 13662848 (63.89%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 38 0.00% 19 23 0.00% 20 46 0.00% 21 62 0.00% 22 57 0.00% 23 68 0.00% 24 143 0.00% 25 107 0.00% 26 79 0.00% 27 178 0.00% 28 199 0.00% 29 216 0.00% 30 449 0.00% 31 312 0.00% 32 410 0.00% 33 422 0.00% 34 424 0.00% 35 319 0.00% 36 510 0.00% 37 546 0.00% 38 605 0.00% 39 651 0.00% 40 789 0.00% 41 936 0.00% 42 922 0.00% 43 994 0.00% 44 1022 0.00% 45 1209 0.01% 46 1235 0.01% 47 1374 0.01% 48 1701 0.01% 49 1767 0.01% 50 2061 0.01% 51 2334 0.01% 52 2634 0.01% 53 3141 0.01% 54 3192 0.01% 55 3621 0.02% 56 3829 0.02% 57 4219 0.02% 58 4584 0.02% 59 5289 0.02% 60 5621 0.03% 61 6914 0.03% 62 7999 0.04% 63 8441 0.04% 64 9181 0.04% 65 9936 0.05% 66 10201 0.05% 67 11479 0.05% 68 13157 0.06% 69 13795 0.06% 70 14727 0.07% 71 16588 0.08% 72 19194 0.09% 73 22390 0.10% 74 24010 0.11% 75 24494 0.11% 76 25251 0.12% 77 28012 0.13% 78 29779 0.14% 79 35820 0.17% 80 36982 0.17% 81 40774 0.19% 82 43101 0.20% 83 45632 0.21% 84 50899 0.24% 85 54102 0.25% 86 57266 0.27% 87 59806 0.28% 88 64771 0.30% 89 66121 0.31% 90 67323 0.31% 91 72255 0.34% 92 77551 0.36% 93 84846 0.40% 94 82119 0.38% 95 88429 0.41% 96 91429 0.43% 97 94175 0.44% 98 99562 0.47% 99 103826 0.49% 100 102324 0.48% 101 102543 0.48% 102 102485 0.48% 103 102331 0.48% 104 104956 0.49% 105 100634 0.47% 106 103458 0.48% 107 109690 0.51% 108 107378 0.50% 109 116354 0.54% 110 112611 0.53% 111 114049 0.53% 112 115651 0.54% 113 106902 0.50% 114 112295 0.53% 115 116891 0.55% 116 117900 0.55% 117 111642 0.52% 118 111595 0.52% 119 110637 0.52% 120 120299 0.56% 121 117282 0.55% 122 115984 0.54% 123 121920 0.57% 124 118896 0.56% 125 123125 0.58% 126 124666 0.58% 127 125076 0.58% 128 119361 0.56% 129 124823 0.58% 130 118831 0.56% 131 122105 0.57% 132 120672 0.56% 133 123004 0.58% 134 122045 0.57% 135 120575 0.56% 136 123946 0.58% 137 121850 0.57% 138 123972 0.58% 139 121247 0.57% 140 118721 0.56% 141 124539 0.58% 142 121294 0.57% 143 123794 0.58% 144 129722 0.61% 145 123814 0.58% 146 123143 0.58% 147 121390 0.57% 148 119687 0.56% 149 115607 0.54% 150 117771 0.55% 151 13662848 63.89% 21385010 reads passed initial QC criterion=sequence-density sequence-density=3.46 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=24 prefix-density=3.41 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.39 sequence-density-rank=27 fanout-score=29.35 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=29.4 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGACAGGATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=sequence-density sequence-density=2.89 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=30 prefix-density=3.02 prefix-fanout=1.9 sequence=AGCCAGAGGAAA criterion=fanout-score sequence-density=0.03 sequence-density-rank=33 fanout-score=61.40 fanout-score-rank=1 prefix-density=1.55 prefix-fanout=1.0 sequence=GTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTATAACGAGCGAAACCCTCGTTTTGTGTTGCTGAGACATGCGCCTAAGGAGAAATTGCCACCGAAGTGAGCCGAGGAGCCGAGTGACGTGCCAGCGCTACTACTTGATTGAGTGCCAGCACGTAGCTGTGCTTTCAGCAAGAATTTCACCATTGGGAGCCGGTGCCTTTCGAAGCACTTTCACGTGTGAACCGAAGTCGTCTTGCCGAACTCAAGACCCACGGAGACCTACCTATAGTGACGTCAAAGTACCAGTGAGCATGGAGGTTTGGTTAGGCTTGGTTACGACGACGTCGAGTTGGCGGCGGAGGAAGACTCGGCATGAAGGCCAGCCGCCCGGTGGTGTGGTACGTAGTGGTAATAGTACGCGCCCCGCTCCGAAACAAAGAAAAAGGTGCGTGCCGCACTCACGAGGGACTGCCAGTGAGATACTGGAGGAAGGTGGGGATGACGTCAAGTCCGCATGGCCCTTATGGGC Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x GTATTTAGCCTTG -y AGCCAGAGGAAA -o SRR13165360 SRR13165360_1.fastq SRR13165360_2.fastq Input file: SRR13165360_1.fastq Paired file: SRR13165360_2.fastq trimmed: SRR13165360-trimmed-pair1.fastq, SRR13165360-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): GTATTTAGCCTTG -- paired 3' end adapter sequence (-y): AGCCAGAGGAAA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:21:55 2024 >> started Sat Dec 7 16:22:54 2024 >> done (58.591s) 10692505 read pairs processed; of these: 451 ( 0.00%) short read pairs filtered out after trimming by size control 1751 ( 0.02%) empty read pairs filtered out after trimming by size control 10690303 (99.98%) read pairs available; of these: 90 ( 0.00%) trimmed read pairs available after processing 10690213 (100.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 23 0.00% 19 10 0.00% 20 27 0.00% 21 34 0.00% 22 20 0.00% 23 32 0.00% 24 72 0.00% 25 53 0.00% 26 44 0.00% 27 70 0.00% 28 91 0.00% 29 107 0.00% 30 222 0.00% 31 152 0.00% 32 218 0.00% 33 208 0.00% 34 201 0.00% 35 154 0.00% 36 260 0.00% 37 266 0.00% 38 293 0.00% 39 337 0.00% 40 395 0.00% 41 444 0.00% 42 432 0.00% 43 516 0.00% 44 516 0.00% 45 606 0.01% 46 628 0.01% 47 669 0.01% 48 853 0.01% 49 902 0.01% 50 1036 0.01% 51 1162 0.01% 52 1351 0.01% 53 1598 0.01% 54 1574 0.01% 55 1811 0.02% 56 1937 0.02% 57 2066 0.02% 58 2266 0.02% 59 2577 0.02% 60 2715 0.03% 61 3461 0.03% 62 3985 0.04% 63 4203 0.04% 64 4606 0.04% 65 4837 0.05% 66 5163 0.05% 67 5729 0.05% 68 6401 0.06% 69 6943 0.06% 70 7216 0.07% 71 8306 0.08% 72 9526 0.09% 73 11084 0.10% 74 11976 0.11% 75 12095 0.11% 76 12581 0.12% 77 14021 0.13% 78 14882 0.14% 79 17781 0.17% 80 18400 0.17% 81 20239 0.19% 82 21403 0.20% 83 22757 0.21% 84 25449 0.24% 85 26940 0.25% 86 28763 0.27% 87 30067 0.28% 88 32436 0.30% 89 33195 0.31% 90 33607 0.31% 91 36366 0.34% 92 38806 0.36% 93 42697 0.40% 94 40953 0.38% 95 44247 0.41% 96 45925 0.43% 97 47153 0.44% 98 49748 0.47% 99 51736 0.48% 100 51244 0.48% 101 51003 0.48% 102 51113 0.48% 103 51256 0.48% 104 52175 0.49% 105 50143 0.47% 106 51915 0.49% 107 54852 0.51% 108 53718 0.50% 109 57941 0.54% 110 56114 0.52% 111 57000 0.53% 112 58408 0.55% 113 53225 0.50% 114 56343 0.53% 115 58283 0.55% 116 59078 0.55% 117 55980 0.52% 118 55508 0.52% 119 55262 0.52% 120 59930 0.56% 121 58338 0.55% 122 58222 0.54% 123 60992 0.57% 124 59380 0.56% 125 61606 0.58% 126 62510 0.58% 127 62511 0.58% 128 59642 0.56% 129 62571 0.59% 130 59618 0.56% 131 60840 0.57% 132 60264 0.56% 133 61618 0.58% 134 61373 0.57% 135 60221 0.56% 136 62161 0.58% 137 60819 0.57% 138 62176 0.58% 139 60721 0.57% 140 59401 0.56% 141 62401 0.58% 142 60299 0.56% 143 62073 0.58% 144 64910 0.61% 145 61903 0.58% 146 61457 0.57% 147 60395 0.56% 148 60197 0.56% 149 57748 0.54% 150 58591 0.55% 151 6830223 63.89% criterion=sequence-density sequence-density=3.40 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=24 prefix-density=3.36 prefix-fanout=2.0 sequence=GTATTTAGCCTTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=250.34 fanout-score-rank=1 prefix-density=1.35 prefix-fanout=1.0 sequence=CTCGCTTTCTTTTCTTCAAAAATTCTTATATGTTAGCGGAAAAACCTTATCCATTAATAGCGGGAACTTCAAGAGCAGCTAGATCTAGAGGGAAGTTGTGAGCATTACGTTCGTGCATTACTTCCATACCAAGATTAGCACGGTTGATGATATCAGCCCAAGTATTAATAACGCGACCTTGACTATCAACTACAGATTGGTTGAAATTGAATCCATTTAGGTTGAACGCCATAGTACTAATACCTAAAGCAGTGAACCAGATTCCTACTACAGGCCAAGCAGCCAAGAAGAAGTGTAAAGAACGAGAGTTGTTGAAACTAGCATATTGGAAGATTAATCGGCCAAAATAACCATGAGCAGCCACAATATTATAAGTCTCTTCCTCTTGACCAAATTTGTAACCCTCATTAGCAGATTCATTTTCAGTAGTTTCCCTGATCAAACTAGAGGTTACCAAGGAACCATGCATAGCACTGAATAGGGAACCGCCGAAAACACCAGCTACACCTAA criterion=sequence-density sequence-density=2.87 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=30 prefix-density=3.01 prefix-fanout=2.0 sequence=AGCCAGAGGAAA criterion=fanout-score sequence-density=0.03 sequence-density-rank=33 fanout-score=58.75 fanout-score-rank=1 prefix-density=1.55 prefix-fanout=1.0 sequence=GTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTATAACGAGCGAAACCCTCGTTTTGTGTTGCTGAGACATGCGCCTAAGGAGAAATTGCCACCGAAGTGAGCCGAGGAGCCGAGTGACGTGCCAGCGCTACTACTTGATTGAGTGCCAGCACGTAGCTGTGCTTTCAGCAAGAATTTCACCATTGGGAGCCGGTGCCTTTCGAAGCACTTTCACGTGTGAACCGAAGTCGTCTTGCCGAACTCAAGACCCACGGAGACCTACCTATAGTGACGTCAAAGTACCAGTGAGCATGGAGGTTTGGTTAGGCTTGGTTACGACGACGTCGAGTTGGCGGCGGAGGAAGACTCGGCATGAAGGCCAGCCGCCCGGTGGTGTGGTACGTAGTGGTAATAGTACGCGCCCCGCTCCGAAACAAAGAAAAAGGTGCGTGCCGCACTCACGAGGGACTGCCAGTGAGATACTGGAGGAAGGTGGGGATGACGTCAAGTCCGCATGGCCCTTATGGGC SRR13165360 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:31:17 Started mapping on | Dec 07 16:31:18 Finished on | Dec 07 16:58:47 Mapping speed, Million of reads per hour | 46.68 Number of input reads | 21382808 Average input read length | 277 UNIQUE READS: Uniquely mapped reads number | 5561567 Uniquely mapped reads % | 26.01% Average mapped length | 287.20 Number of splices: Total | 1666471 Number of splices: Annotated (sjdb) | 1549998 Number of splices: GT/AG | 1633707 Number of splices: GC/AG | 20168 Number of splices: AT/AC | 886 Number of splices: Non-canonical | 11710 Mismatch rate per base, % | 0.18% Deletion rate per base | 0.01% Deletion average length | 2.02 Insertion rate per base | 0.00% Insertion average length | 2.05 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 5924058 % of reads mapped to multiple loci | 27.70% Number of reads mapped to too many loci | 1698070 % of reads mapped to too many loci | 7.94% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.93% % of reads unmapped: other | 34.41% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9897329 9897329 9897329 N_multimapping 5924058 5924058 5924058 N_noFeature 3405308 5486645 3434942 N_ambiguous 70725 506 25483 UnstrandedReadsAssigned:2085534 PositiveStrandReadsAssigned:74416 NegativeStrandReadsAssigned:2101142 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=122 echo kmer=117 SRR13165360 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165360-trimmed-pair1.fastq SRR13165360-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,382,808 reads, 3,258,326 reads pseudoaligned [quant] estimated average fragment length: 190.138 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,020 rounds 52973 SRR13165360.ke.tsv 35125 SRR13165360.se.tsv 88098 total ==> SRR13165360.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 746.988 0 0 PNS24247 1044 854.862 8.52693 1.96283 PNS24249 1928 1738.86 7.6099 0.86119 PNS24246 1044 854.862 8.52693 1.96283 PNS24248 1044 854.862 8.52693 1.96283 PNS24244 1471 1281.86 13.8093 2.1199 PNS24243 293 129.82 0 0 KQK14069 1603 1413.86 63.0242 8.77173 KQK14071 474 292.18 1.9758 1.33069 ==> SRR13165360.se.tsv <== BRADI_1g14170v3 60 BRADI_1g53295v3 14 BRADI_1g59795v3 8 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 40 BRADI_1g74790v3 57 BRADI_1g09890v3 1 BRADI_1g77505v3 38 BRADI_1g48960v3 0 SRR13165360 completed mapping pipeline successfully