Starting /dee2/code/volunteer_pipeline.sh SRR13165361 current disk space = 1541874221056 free memory = 1470153404 SRR13165361 SRAfilesize 2096c2dc999900909026c7ae2a5fe5c4 SRR13165361.sra SRR13165361.sra file validated SRR13165361 is paired end SRR13165361 is conventional basespace SRR13165361 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165361_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.551 37.0 37.0 37.0 37.0 37.0 2 36.17425 37.0 37.0 37.0 37.0 37.0 3 36.41 37.0 37.0 37.0 37.0 37.0 4 36.6215 37.0 37.0 37.0 37.0 37.0 5 36.6425 37.0 37.0 37.0 37.0 37.0 6 36.554 37.0 37.0 37.0 37.0 37.0 7 36.3955 37.0 37.0 37.0 37.0 37.0 8 36.56 37.0 37.0 37.0 37.0 37.0 9 36.528 37.0 37.0 37.0 37.0 37.0 10-14 36.5327 37.0 37.0 37.0 37.0 37.0 15-19 36.499399999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.491200000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.472899999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.45700000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.399899999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.4229 37.0 37.0 37.0 37.0 37.0 45-49 36.3829 37.0 37.0 37.0 37.0 37.0 50-54 36.3312 37.0 37.0 37.0 37.0 37.0 55-59 36.363600000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.3861 37.0 37.0 37.0 37.0 37.0 65-69 36.301899999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.3153 37.0 37.0 37.0 37.0 37.0 75-79 36.275600000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.193799999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.2184 37.0 37.0 37.0 37.0 37.0 90-94 36.17450000000001 37.0 37.0 37.0 37.0 37.0 95-99 36.169 37.0 37.0 37.0 37.0 37.0 100-104 36.174400000000006 37.0 37.0 37.0 37.0 37.0 105-109 36.1416 37.0 37.0 37.0 37.0 37.0 110-114 36.129599999999996 37.0 37.0 37.0 37.0 37.0 115-119 36.159800000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.9683 37.0 37.0 37.0 37.0 37.0 125-129 36.0317 37.0 37.0 37.0 37.0 37.0 130-134 35.96939999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.8512 37.0 37.0 37.0 37.0 37.0 140-144 35.75429999999999 37.0 37.0 37.0 37.0 37.0 145-149 35.51090000000001 37.0 37.0 37.0 37.0 37.0 150-151 35.393 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 2.0 23 2.0 24 6.0 25 4.0 26 7.0 27 7.0 28 12.0 29 14.0 30 17.0 31 29.0 32 62.0 33 72.0 34 119.0 35 358.0 36 2846.0 37 441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 60.175 10.15 5.0 24.675 2 23.042054898010576 9.695290858725762 32.76252833039537 34.5001259128683 3 20.125 17.1 27.650000000000002 35.125 4 26.650000000000002 23.075000000000003 22.525000000000002 27.750000000000004 5 28.9 27.275 22.475 21.349999999999998 6 24.65 31.15 22.575 21.625 7 19.375 25.85 38.45 16.325 8 19.85 23.875 29.349999999999998 26.924999999999997 9 21.75 21.025 31.075000000000003 26.150000000000002 10-14 23.24 27.18 25.215 24.365000000000002 15-19 24.265 24.740000000000002 25.505 25.490000000000002 20-24 24.065 25.235000000000003 24.8 25.900000000000002 25-29 24.22 26.025 24.59 25.165 30-34 23.630000000000003 26.135 23.845 26.39 35-39 23.74 25.669999999999998 24.605 25.985000000000003 40-44 24.224999999999998 25.305 24.75 25.72 45-49 24.12 25.669999999999998 24.755 25.455 50-54 24.36 25.040000000000003 25.069999999999997 25.53 55-59 24.515 24.884999999999998 24.585 26.015 60-64 24.29 25.44 24.88 25.39 65-69 24.585 25.66 24.035 25.72 70-74 24.59 24.85 24.709999999999997 25.85 75-79 23.805 25.66 24.44 26.095000000000002 80-84 24.795 24.595 24.84 25.77 85-89 24.965 24.87 24.545 25.619999999999997 90-94 24.91 24.965 24.224999999999998 25.900000000000002 95-99 24.025 25.825 23.68 26.47 100-104 24.615000000000002 25.455 24.67 25.259999999999998 105-109 25.035 25.169999999999998 23.674999999999997 26.119999999999997 110-114 25.525 24.13 24.32 26.025 115-119 24.8 26.224999999999998 23.055 25.919999999999998 120-124 24.965 25.515 23.29 26.229999999999997 125-129 25.385 25.385 23.455000000000002 25.775 130-134 25.695 25.645 23.275000000000002 25.385 135-139 25.845000000000002 25.05 23.465 25.64 140-144 25.009999999999998 25.790000000000003 23.385 25.814999999999998 145-149 25.679999999999996 25.224999999999998 23.9 25.195 150-151 25.650000000000002 25.575 22.5 26.275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 2.0 26 3.0 27 2.0 28 4.5 29 7.5 30 9.5 31 8.5 32 13.0 33 22.5 34 31.5 35 53.5 36 58.5 37 43.5 38 67.0 39 97.0 40 106.5 41 131.0 42 156.0 43 167.0 44 171.5 45 181.0 46 198.5 47 200.0 48 180.0 49 159.0 50 152.0 51 147.5 52 131.5 53 119.5 54 114.5 55 86.0 56 74.5 57 93.0 58 96.0 59 83.0 60 69.0 61 71.0 62 61.5 63 59.5 64 69.5 65 71.5 66 61.5 67 58.5 68 64.5 69 54.0 70 49.0 71 36.5 72 23.5 73 16.0 74 16.0 75 17.5 76 8.0 77 7.0 78 6.5 79 2.5 80 1.0 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.7250000000000001 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.3 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8243430152144 53.37499999999999 2 18.01521438450899 26.05 3 5.8437067773167355 12.675 4 1.313969571230982 3.8 5 0.5186721991701244 1.875 6 0.3457814661134163 1.5 7 0.1037344398340249 0.525 8 0.034578146611341634 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACCTGCTGGGTCTTGCCGTCGTCGGTGACCCGGACGTGGTCGCCGGAC 8 0.2 No Hit GTCACGTTCGTCGGCGGGCCCGCGTCGGCGGCGGCGGCTCCGGGACCGTC 7 0.17500000000000002 No Hit GCGAGGAAAGGATTGACCCGGACCCAGAGGAGGGAGAGGATCGAAGCCAG 7 0.17500000000000002 No Hit GCCCTTGGGGGCGCAAGCGCCCCTAACGTGGGTCGGGGCAGGCGGCGGGC 7 0.17500000000000002 No Hit GCTTCCTGCGCGGCGACCAGGCCGCTGATGTGCGCCATGTCGCAGAGGAG 6 0.15 No Hit GTCGGGAGGAAGGAGATGATGTCGCCGAGGATGGCGTCGGGGAGTTTGCT 6 0.15 No Hit GTTGGTGTCAAACCACTTGGTCATCTCCATAGCAGGGACAGTGGCATTTC 6 0.15 No Hit GTTTTGTTAATCATTGCAATAATACTTGGAATTTTGGCTTTGATCACAGC 6 0.15 No Hit GGACGCGAACCACAGCTGCCTGTTATCATTTTGCTTGGCAGGGGGGCTGG 6 0.15 No Hit GGCTCGTCGAGGGTGTAGGAGACGACCCCCTTGGGGATGATGCCCGGGGG 6 0.15 No Hit GCCTCATCCTCATAGTAATGTGCAGTAACCCCTGCAGCTTGTGCAGCATT 6 0.15 No Hit GTCTCTTATCCGCTTCAGGGTGTAGGCTTGGCAAACATTCAAGGTTGTGA 6 0.15 No Hit GTTCATATCAAATAGTTCCATAATGGCAACAAGCAAATAAACAAACTACT 6 0.15 No Hit CAAATAAATAGCACCAACAAAAACAGGTTCTAGAGCTGACAAAATCAAAT 6 0.15 No Hit GCTCGTACTCGCCGTCGGCGCCGGTTGGGGTTACCACCGTCTCCGCCGAG 5 0.125 No Hit CCCCGGCTTGCACTCCTCCGTCATCTCCAGGTGCTCGGCCGCGCAGCGCA 5 0.125 No Hit GCAGCACCTTCCTTTCCTCCAACACAAGTCCATCTCTTCACCATGGCCTC 5 0.125 No Hit CGTATAAGAGCATTCAAGATGAAGAAAATAACAAAAGCAATCCCCGGAAA 5 0.125 No Hit GCTGTATTAATCCTCCTTTAGTGAAGTTGAAAGACTCGAATTGAGGTAAA 5 0.125 No Hit CTCAGCACCGACGTCTCCCTCAGAGAATCGAACATGTACTCGTAGCAGAT 5 0.125 No Hit GGATGGCATCGCCCGAGGGGACCTTCTGGAACATGTCACCGCCCACGTGG 5 0.125 No Hit GCCGATGCACATACACAGCAACAAAGTGTTCTTGGTACAAGAATCGACCG 5 0.125 No Hit ATACGTACACCTCTTATTCGAACAGGGACCAAGTCGAAGGCACCCTTCAT 5 0.125 No Hit CGGGCAATGTCGCCGGTTCACTTCCAGAGAACATCATAATAACGGGCTCC 5 0.125 No Hit GCCCTATGCAGACATGGTATGGGGTATTTGCAGCAATAGGCTCAATCTGG 5 0.125 No Hit GCCAACAACAACAACACGAACACTACCCAGCTCTCCCAAATCAAGCATTC 5 0.125 No Hit GCCACCTGCATCACGCAGTTCCTCACCGCTGTCTTCATGTTCTCCTCGAG 5 0.125 No Hit GCCATTTCACAATCCTCCCTATCAGCTTCTCAACCTCCTCATCGGAGAGG 5 0.125 No Hit GCCAGTTTGGAATGTAGTCCCTGTTAAAGACCTCATCGCGTTGCATATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.037500000000000006 0.0 0.0 0.0 0.0 48-49 0.0625 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.0875 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.3875 0.0 0.0 0.0 0.0 80-81 0.48750000000000004 0.0 0.0 0.0 0.0 82-83 0.6000000000000001 0.0 0.0 0.0 0.0 84-85 0.7375 0.0 0.0 0.0 0.0 86-87 1.0125 0.0 0.0 0.0 0.0 88-89 1.1625 0.0 0.0 0.0 0.0 90-91 1.4125 0.0 0.0 0.0 0.0 92-93 1.6125 0.0 0.0 0.0 0.0 94-95 2.1125 0.0 0.0 0.0 0.0 96-97 2.4375 0.0 0.0 0.0 0.0 98-99 2.7375 0.0 0.0 0.0 0.0 100-101 3.0375 0.0 0.0 0.0 0.0 102-103 3.575 0.0 0.0 0.0 0.0 104-105 4.025 0.0 0.0 0.0 0.0 106-107 4.375 0.0 0.0 0.0 0.0 108-109 5.1375 0.0 0.0 0.0 0.0 110-111 5.65 0.0 0.0 0.0 0.0 112-113 5.862500000000001 0.0 0.0 0.0 0.0 114-115 6.2125 0.0 0.0 0.0 0.0 116-117 6.699999999999999 0.0 0.0 0.0 0.0 118-119 7.324999999999999 0.0 0.0 0.0 0.0 120-121 7.887499999999999 0.0 0.0 0.0 0.0 122-123 8.55 0.0 0.0 0.0 0.0 124-125 9.35 0.0 0.0 0.0 0.0 126-127 10.175 0.0 0.0 0.0 0.0 128-129 10.837499999999999 0.0 0.0 0.0 0.0 130-131 11.45 0.0 0.0 0.0 0.0 132-133 11.925 0.0 0.0 0.0 0.0 134-135 12.875 0.0 0.0 0.0 0.0 136-137 13.7625 0.0 0.0 0.0 0.0 138-139 14.7 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAAA 10 0.006830828 145.0 7 GTCAAAA 10 0.006830828 145.0 1 GATGCAG 10 0.006830828 145.0 145 ATAAAAT 10 0.006830828 145.0 7 AAAATAA 10 0.006830828 145.0 4 GACTAAA 10 0.006830828 145.0 145 >>END_MODULE SRR13165361 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165361_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.84025 37.0 37.0 37.0 37.0 37.0 2 36.1875 37.0 37.0 37.0 37.0 37.0 3 36.0555 37.0 37.0 37.0 37.0 37.0 4 36.0795 37.0 37.0 37.0 37.0 37.0 5 36.212 37.0 37.0 37.0 37.0 37.0 6 36.1145 37.0 37.0 37.0 37.0 37.0 7 36.0935 37.0 37.0 37.0 37.0 37.0 8 36.2035 37.0 37.0 37.0 37.0 37.0 9 36.0515 37.0 37.0 37.0 37.0 37.0 10-14 36.051300000000005 37.0 37.0 37.0 37.0 37.0 15-19 35.9959 37.0 37.0 37.0 37.0 37.0 20-24 35.93605 37.0 37.0 37.0 37.0 37.0 25-29 35.89235000000001 37.0 37.0 37.0 37.0 37.0 30-34 35.890249999999995 37.0 37.0 37.0 37.0 37.0 35-39 35.78915 37.0 37.0 37.0 37.0 37.0 40-44 35.79965 37.0 37.0 37.0 37.0 37.0 45-49 35.76605 37.0 37.0 37.0 37.0 37.0 50-54 35.70795 37.0 37.0 37.0 37.0 37.0 55-59 35.733250000000005 37.0 37.0 37.0 37.0 37.0 60-64 35.70375 37.0 37.0 37.0 37.0 37.0 65-69 35.7032 37.0 37.0 37.0 37.0 37.0 70-74 35.64834999999999 37.0 37.0 37.0 37.0 37.0 75-79 35.60185 37.0 37.0 37.0 37.0 37.0 80-84 35.62785 37.0 37.0 37.0 37.0 37.0 85-89 35.52535 37.0 37.0 37.0 37.0 37.0 90-94 35.53805 37.0 37.0 37.0 37.0 37.0 95-99 35.53065 37.0 37.0 37.0 37.0 37.0 100-104 35.560950000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.53985 37.0 37.0 37.0 37.0 37.0 110-114 35.358850000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.37445 37.0 37.0 37.0 37.0 37.0 120-124 35.254650000000005 37.0 37.0 37.0 34.6 37.0 125-129 35.19365 37.0 37.0 37.0 29.8 37.0 130-134 35.09694999999999 37.0 37.0 37.0 27.4 37.0 135-139 35.12325 37.0 37.0 37.0 27.4 37.0 140-144 34.90835 37.0 37.0 37.0 25.0 37.0 145-149 34.79025 37.0 37.0 37.0 25.0 37.0 150-151 34.5745 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 4.0 13 11.0 14 10.0 15 10.0 16 6.0 17 4.0 18 4.0 19 1.0 20 1.0 21 7.0 22 8.0 23 9.0 24 5.0 25 10.0 26 7.0 27 9.0 28 14.0 29 28.0 30 25.0 31 33.0 32 63.0 33 91.0 34 238.0 35 604.0 36 2580.0 37 215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.58657954259864 20.960040211108318 5.554159336516713 18.899220909776325 2 30.225 21.75 23.95 24.075 3 24.45 23.1 29.175 23.275000000000002 4 27.55 31.574999999999996 18.975 21.9 5 29.025000000000002 31.55 19.325 20.1 6 25.1 34.525 18.05 22.325 7 23.325000000000003 19.225 33.900000000000006 23.549999999999997 8 24.925 22.425 24.0 28.65 9 24.825 21.275 26.25 27.650000000000002 10-14 26.590000000000003 24.945 22.78 25.685000000000002 15-19 26.57 24.085 23.7 25.645 20-24 26.10457843382537 24.263197398048536 24.89867400550413 24.733550162621967 25-29 26.099574681010758 24.288216162121593 23.692769577182887 25.91943957968476 30-34 26.760070052539405 24.49837378033525 23.867900925694272 24.873655241431074 35-39 26.459844883662747 25.023767825869403 23.932949712284213 24.583437578183638 40-44 25.969477107830873 25.303977983487613 23.777833375031275 24.948711533650236 45-49 26.82011508631474 23.837878408806603 24.193144858643983 25.14886164623468 50-54 26.189642231673755 25.04378283712785 24.313234926194646 24.453340005003753 55-59 26.970227670753065 24.36827620715537 23.522641981486114 25.138854140605453 60-64 25.283962972229173 24.258193645233924 25.1138353765324 25.344008006004504 65-69 26.210726435861513 24.444666800080046 23.989393636181706 25.355213127876723 70-74 26.705028771578682 24.843632724543408 23.74781085814361 24.7035276457343 75-79 25.69427070302727 24.733550162621967 24.303227420565424 25.268951713785338 80-84 26.14961220915687 25.879409557167875 23.81285964473355 24.158118588941708 85-89 26.32974731048286 24.628471353515135 23.96297222917188 25.078809106830125 90-94 26.229672254190646 25.238929196897676 24.06805103827871 24.463347510632975 95-99 26.509882411808857 24.798598949211907 23.677758318739052 25.01376032024018 100-104 26.464848636477356 24.998749061796346 24.34826119589692 24.188141105829374 105-109 26.464848636477356 24.818613960470355 24.043032274205654 24.673505128846635 110-114 26.79509632224168 25.469101826369776 23.967975981986488 23.767825869402053 115-119 27.72079059294471 24.858643982987243 23.827870903177384 23.592694520890667 120-124 26.925193895421568 25.509131848886664 23.387540655491616 24.178133600200148 125-129 28.181135851888918 25.649236927695775 23.2424318238679 22.92719539654741 130-134 28.98173630222667 25.088816612459347 22.40680510382787 23.522641981486114 135-139 29.306980235176383 25.00375281461096 23.212409306980238 22.476857643232425 140-144 30.167625719289465 24.723542656992745 22.727045283962973 22.381786339754818 145-149 31.49362021516137 24.69352014010508 22.531898924193143 21.280960720540406 150-151 32.161621215911936 24.73104828621466 22.166624968726545 20.94070552914686 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.5 10 1.0 11 1.0 12 0.5 13 0.5 14 2.0 15 1.5 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 1.0 22 1.5 23 2.5 24 2.0 25 1.5 26 3.5 27 3.0 28 3.5 29 3.5 30 6.5 31 9.5 32 12.5 33 15.5 34 14.0 35 25.0 36 35.0 37 46.5 38 64.0 39 77.5 40 106.0 41 118.5 42 136.0 43 180.5 44 177.5 45 178.5 46 194.0 47 177.0 48 157.0 49 152.5 50 153.0 51 121.0 52 102.0 53 121.0 54 134.5 55 113.0 56 95.0 57 97.5 58 85.5 59 73.0 60 82.0 61 84.0 62 78.0 63 77.0 64 71.0 65 71.5 66 74.5 67 74.5 68 76.5 69 67.5 70 47.5 71 46.0 72 40.5 73 25.0 74 16.5 75 9.0 76 5.5 77 6.0 78 6.0 79 4.5 80 2.5 81 2.0 82 1.5 83 1.5 84 2.0 85 1.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.5 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.075 25-29 0.075 30-34 0.075 35-39 0.075 40-44 0.075 45-49 0.075 50-54 0.075 55-59 0.075 60-64 0.075 65-69 0.06 70-74 0.075 75-79 0.075 80-84 0.075 85-89 0.075 90-94 0.075 95-99 0.075 100-104 0.075 105-109 0.075 110-114 0.075 115-119 0.075 120-124 0.075 125-129 0.075 130-134 0.075 135-139 0.075 140-144 0.075 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.175 #Duplication Level Percentage of deduplicated Percentage of total 1 75.02562350529553 54.900000000000006 2 17.287324906047147 25.3 3 5.329689101469081 11.700000000000001 4 1.3324222753672703 3.9 5 0.5807994533652203 2.125 6 0.30748206354629315 1.35 7 0.10249402118209772 0.525 8 0.0341646737273659 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACTGCACTCTCTCTCACCACCAACACAGAGGAATTAAGCAGAGGAAGA 8 0.2 No Hit GCAAAACAGGACCCCAACAATCGTCATCGTCTGGTCCATCCTTCTGGCTT 7 0.17500000000000002 No Hit CCCACCTTCTTCGCCAACAATGGCGCCGCCGTCGCGGGTCCTCCTCCTAG 7 0.17500000000000002 No Hit GACAGGTTAGTTTTACCCTACTGATGACCGTGCCGCGATAGTAATTCAAC 7 0.17500000000000002 No Hit GGCTGAAGATCTGCAATCCTTTGGGGAGCAGTTGAGAAACAACTTTGAAG 6 0.15 No Hit GCTCTCTCGTCCTTCCTCCAGCCTCCGCCCCCTCGCCGCCGCGCTCCTCC 6 0.15 No Hit CGTGATCCACTTCTGCTACGGAAGTTCTTGGCGATACACAGACGTATTCA 6 0.15 No Hit GGACAATAGCCCTAGTCCCAAGAAGAGGAAACTAGCGGCGTCAATGATCC 6 0.15 No Hit ATCTCCCCGAAAGCAGGAGCTAGAGGAGGAGCTTGCAGGAACAATGGCGG 6 0.15 No Hit GTTCAGTTGAATTGAGTCTGTGTCTGTGTTTTCTGACTTAATTCTGTGAG 6 0.15 No Hit TGACAAGCCAAACTCAATCATATTGGCCATCTCTCCAGCAAACCAAGATA 6 0.15 No Hit CCTGCCGGTGAACCCGGAGGCCACCCCAAAGGCACAAGGGGTGTTCCACG 6 0.15 No Hit CATGGACTTCCGCCCCAAGCTCATCATCTGCGGCGGCAGCGCCTACCCCA 6 0.15 No Hit AGCAGACTGCGGCTCTTCCTAAGTCCAGGATACCCTTACGAAGAAATATT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit CAGCGACTGGATCCGTTCACAAGAATTCCCAAGCGATATTACCATCCAAG 5 0.125 No Hit GCTCATCATCTGGGTTTGATGGCCTGACGGAGTAAAGTGATCCGTGGTCT 5 0.125 No Hit TATGTTGGCACCGGAGTCCAATTCTTTGGCATGCTGCTCGTTACGATGAT 5 0.125 No Hit AGTGACTCGGGAGGATGGAGCAAGACATACAGCTTTATTTCACGTGACAG 5 0.125 No Hit AGAAGAAGTCACCAAACTTATGAATCAAAACGAGAAGCTTGCTTCTGAGT 5 0.125 No Hit GCGAGGGTATGTGGAGGCCCGGACTGTGGATTCCGGGAATACTTCTGGGG 5 0.125 No Hit GCTGGTGTGGTGTATACGTAGTAGTATACGATGCTCCGCATTTGATTGAT 5 0.125 No Hit AGAGCCCTCGCCAAACACTGACCTCAGATCTCTCTCGCCTTACGCCGCCG 5 0.125 No Hit GCAGTTCTTGGGCAACCCGGTGACCAACCACGTCCAGAGCGCGGAGCAGC 5 0.125 No Hit CACACACACACACAGGTGTGTAAGCCAGCTCAGTAGCTAGCTCCTCGAGC 5 0.125 No Hit GGGGAAGACGGTGCTGGAGCTCGGCGCCGGGATCGGGCGCTTCACCGGGG 5 0.125 No Hit TAGGGGGGTCCATGGATCCCTTGCTCCCTGAGCTTCTTAGCTATGGTGTA 5 0.125 No Hit GCAAATTCTTCGACGACAATGTTGATAAGGAGCATTACCACTGCAAGGAC 5 0.125 No Hit CGGGGATGATCTGCTCACACCTGGTAAATCTTTCCACGCTCTTCGGTGCA 5 0.125 No Hit TTTTTTTTTTTTGCATCAAAGCTTTTTCTATACAACCGTTTGTGTTTGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.037500000000000006 0.0 0.0 0.0 0.0 48-49 0.0625 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.0875 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.7125 0.0 0.0 0.0 0.0 86-87 0.9875 0.0 0.0 0.0 0.0 88-89 1.1375 0.0 0.0 0.0 0.0 90-91 1.3875 0.0 0.0 0.0 0.0 92-93 1.5875 0.0 0.0 0.0 0.0 94-95 2.0875 0.0 0.0 0.0 0.0 96-97 2.4124999999999996 0.0 0.0 0.0 0.0 98-99 2.7125 0.0 0.0 0.0 0.0 100-101 3.025 0.0 0.0 0.0 0.0 102-103 3.575 0.0 0.0 0.0 0.0 104-105 4.025 0.0 0.0 0.0 0.0 106-107 4.375 0.0 0.0 0.0 0.0 108-109 5.1125 0.0 0.0 0.0 0.0 110-111 5.65 0.0 0.0 0.0 0.0 112-113 5.8375 0.0 0.0 0.0 0.0 114-115 6.1875 0.0 0.0 0.0 0.0 116-117 6.6625 0.0 0.0 0.0 0.0 118-119 7.262499999999999 0.0 0.0 0.0 0.0 120-121 7.8375 0.0 0.0 0.0 0.0 122-123 8.5125 0.0 0.0 0.0 0.0 124-125 9.325 0.0 0.0 0.0 0.0 126-127 10.175 0.0 0.0 0.0 0.0 128-129 10.8125 0.0 0.0 0.0 0.0 130-131 11.425 0.0 0.0 0.0 0.0 132-133 11.9 0.0 0.0 0.0 0.0 134-135 12.837499999999999 0.0 0.0 0.0 0.0 136-137 13.75 0.0 0.0 0.0 0.0 138-139 14.7 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCTCG 10 0.006830828 145.0 5 AGCTCGG 10 0.006830828 145.0 6 TCGGCAT 10 0.006830828 145.0 9 TTATCAT 10 0.006830828 145.0 6 ATTATCA 10 0.006830828 145.0 5 ATCACAG 10 0.006830828 145.0 1 TTGGTCC 10 0.006830828 145.0 145 TCACAGC 10 0.006830828 145.0 2 CACAGCT 10 0.006830828 145.0 3 >>END_MODULE Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543481 spots for SRR13165361.sra Written 1543481 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra Read 1543471 spots for SRR13165361.sra Written 1543471 spots for SRR13165361.sra SRR ids: ['SRR13165361.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9pwxfbwj SRR13165361.sra spots: 30869430 blocks: [[1, 1543471], [1543472, 3086942], [3086943, 4630413], [4630414, 6173884], [6173885, 7717355], [7717356, 9260826], [9260827, 10804297], [10804298, 12347768], [12347769, 13891239], [13891240, 15434710], [15434711, 16978181], [16978182, 18521652], [18521653, 20065123], [20065124, 21608594], [21608595, 23152065], [23152066, 24695536], [24695537, 26239007], [26239008, 27782478], [27782479, 29325949], [29325950, 30869430]] SRR13165361 file size 10469082 SRR13165361 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165361 SRR13165361_1.fastq SRR13165361_2.fastq Input file: SRR13165361_1.fastq Paired file: SRR13165361_2.fastq trimmed: SRR13165361-trimmed-pair1.fastq, SRR13165361-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:08:29 2024 >> started Sat Dec 7 16:09:07 2024 >> done (38.394s) 30869430 read pairs processed; of these: 665 ( 0.00%) short read pairs filtered out after trimming by size control 27802 ( 0.09%) empty read pairs filtered out after trimming by size control 30840963 (99.91%) read pairs available; of these: 5772353 (18.72%) trimmed read pairs available after processing 25068610 (81.28%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 55 0.00% 19 54 0.00% 20 42 0.00% 21 55 0.00% 22 84 0.00% 23 79 0.00% 24 86 0.00% 25 113 0.00% 26 111 0.00% 27 117 0.00% 28 143 0.00% 29 179 0.00% 30 182 0.00% 31 161 0.00% 32 180 0.00% 33 189 0.00% 34 202 0.00% 35 204 0.00% 36 195 0.00% 37 257 0.00% 38 174 0.00% 39 265 0.00% 40 278 0.00% 41 295 0.00% 42 269 0.00% 43 397 0.00% 44 369 0.00% 45 350 0.00% 46 398 0.00% 47 424 0.00% 48 557 0.00% 49 597 0.00% 50 720 0.00% 51 723 0.00% 52 762 0.00% 53 903 0.00% 54 934 0.00% 55 1093 0.00% 56 1146 0.00% 57 1264 0.00% 58 1412 0.00% 59 1591 0.01% 60 1951 0.01% 61 2111 0.01% 62 2603 0.01% 63 2878 0.01% 64 3091 0.01% 65 3297 0.01% 66 3586 0.01% 67 3995 0.01% 68 4375 0.01% 69 4984 0.02% 70 5545 0.02% 71 6401 0.02% 72 7587 0.02% 73 8620 0.03% 74 9226 0.03% 75 10240 0.03% 76 11134 0.04% 77 11864 0.04% 78 13350 0.04% 79 14514 0.05% 80 15752 0.05% 81 17652 0.06% 82 19675 0.06% 83 21311 0.07% 84 23847 0.08% 85 25679 0.08% 86 27199 0.09% 87 29035 0.09% 88 30618 0.10% 89 32044 0.10% 90 35064 0.11% 91 37138 0.12% 92 39405 0.13% 93 42535 0.14% 94 45474 0.15% 95 47650 0.15% 96 50555 0.16% 97 52771 0.17% 98 53717 0.17% 99 55901 0.18% 100 58028 0.19% 101 59884 0.19% 102 62650 0.20% 103 64777 0.21% 104 67316 0.22% 105 69161 0.22% 106 71235 0.23% 107 73428 0.24% 108 75366 0.24% 109 76504 0.25% 110 77227 0.25% 111 79728 0.26% 112 81462 0.26% 113 82485 0.27% 114 85655 0.28% 115 87990 0.29% 116 89646 0.29% 117 91095 0.30% 118 91651 0.30% 119 92858 0.30% 120 94588 0.31% 121 94987 0.31% 122 95679 0.31% 123 97613 0.32% 124 100125 0.32% 125 100031 0.32% 126 103461 0.34% 127 103184 0.33% 128 104023 0.34% 129 106120 0.34% 130 106042 0.34% 131 104876 0.34% 132 107760 0.35% 133 108792 0.35% 134 108443 0.35% 135 110701 0.36% 136 110320 0.36% 137 110932 0.36% 138 112201 0.36% 139 112885 0.37% 140 113400 0.37% 141 113499 0.37% 142 115329 0.37% 143 115641 0.37% 144 117563 0.38% 145 118689 0.38% 146 117992 0.38% 147 120344 0.39% 148 118384 0.38% 149 118803 0.39% 150 119817 0.39% 151 25068610 81.28% 30840963 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=3.37 fanout-score-rank=21 prefix-density=0.33 prefix-fanout=2.5 sequence=TCCTGGATCTTGGCCTTCACGTTGTCGATGGTGTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=44 fanout-score=81.39 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=10.0 sequence=GAGAAGAGGAATAATCATGTCATTACATCACTTGTATATATGCAACCTTTTAAGAGCAGCATTATTAATAGTACAGTTCGGTCCGACAGACGTCGACACAACCATTAGAAATTCAGGGTGATCAGCAGATCATGGAGAAAACGAAAGAAAAACAGAGGAAAAGCCAAGCAAAAAGTACATATGCAGATCGACCAAGGCAGAAGTGGAGATCGATCTCATCTCATAATCTCAAATGGATGGATGATTTGATCTACTTGGTGAACTCGATGGCCCACGCGTTGGCGTAGATGTATGTGGCCTTGACGCCGGTGAACCCGGCGCCCCGGGCGAGCTCCTCGAACTCCCTCTCGTACCTCTCCTTGCCCCCCGGGTTGTGGGCGAGCATGATCATGTCGACGTGGAACACCCCTTGTGCCTTTGGGGTGGCCTCCGGGTTCACCGGCAGGATGCACTCGACGATGACCACTTTCCCGTGTGCGGGGAGCGCGTCGTAGCAGTTCTTGAGCAGCGT criterion=sequence-density sequence-density=0.25 sequence-density-rank=1 fanout-score=8.26 fanout-score-rank=7 prefix-density=0.39 prefix-fanout=5.3 sequence=GGCAAGACCATCACCCTTGAGGTGGAGTCATCTGACACCATCGACAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=42 fanout-score=42.75 fanout-score-rank=1 prefix-density=0.59 prefix-fanout=5.5 sequence=GGAGGAGGTGATGATGCTTTCAACACCTTCTTCAGTGAGACTGGTGCGGGGAAGCATGTCCCCCGTGCTGTCTTTGTGGATCTGGAACCCACTGTGATTGATGAGGTGCGGACTGGCACTTACCGCCAGCTCTTCCACCCTGAGCAGCTCATCAGTGGCAAGGAGGATGCAGCCAACAACTTTGCCCGCGGTCACTATACCATTGGCAAGGAGATTGTTGATCTCTGTCTCGACCGCATCAGGAAGCTTGCAGACAACTGCACTGGTCTCCAAGGCTTCCTTGTGTTCAATGCTGTTGGGGGTGGAACTGGATCCGGTCTTGGTTCTCTTCTCCTTGAGCGGCTCTCTGTTGACTATGGAAAGAAGTCCAAGCTTGGGTTCACCGTGTACCCATCTCCTCAGGTCTCCACCTCTGTCGTTGAGCCATACAACAGTGTCCTGTCCACCCACTCTCTTCTTGAGCACACTGATGTGGCTGTCCTTCTCGACAATGAGGCCATCTATGACATCT SRR13165361 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:11:59 Started mapping on | Dec 07 16:12:00 Finished on | Dec 07 16:15:02 Mapping speed, Million of reads per hour | 610.04 Number of input reads | 30840963 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 29106263 Uniquely mapped reads % | 94.38% Average mapped length | 289.68 Number of splices: Total | 26572579 Number of splices: Annotated (sjdb) | 24960887 Number of splices: GT/AG | 26246305 Number of splices: GC/AG | 266023 Number of splices: AT/AC | 14356 Number of splices: Non-canonical | 45895 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 2.94 Insertion rate per base | 0.01% Insertion average length | 2.27 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 237883 % of reads mapped to multiple loci | 0.77% Number of reads mapped to too many loci | 44581 % of reads mapped to too many loci | 0.14% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.02% % of reads unmapped: other | 0.69% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1497168 1497168 1497168 N_multimapping 237883 237883 237883 N_noFeature 949046 28320639 1213166 N_ambiguous 589280 3954 68613 UnstrandedReadsAssigned:27567937 PositiveStrandReadsAssigned:781670 NegativeStrandReadsAssigned:27824484 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165361 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165361-trimmed-pair1.fastq SRR13165361-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,840,963 reads, 28,252,046 reads pseudoaligned [quant] estimated average fragment length: 241.747 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,109 rounds 52973 SRR13165361.ke.tsv 35125 SRR13165361.se.tsv 88098 total ==> SRR13165361.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 695.867 0 0 PNS24247 1044 803.253 42.4868 2.98317 PNS24249 1928 1687.25 126.787 4.23809 PNS24246 1044 803.253 42.4868 2.98317 PNS24248 1044 803.253 42.4868 2.98317 PNS24244 1471 1230.25 94.7527 4.34383 PNS24243 293 110.156 0 0 KQK14069 1603 1362.25 18.0043 0.745407 KQK14071 474 253.764 0 0 ==> SRR13165361.se.tsv <== BRADI_1g14170v3 18 BRADI_1g53295v3 99 BRADI_1g59795v3 412 BRADI_1g07683v3 0 BRADI_1g00485v3 63 BRADI_1g20270v3 2757 BRADI_1g74790v3 253 BRADI_1g09890v3 51 BRADI_1g77505v3 225 BRADI_1g48960v3 0 SRR13165361 completed mapping pipeline successfully