Starting /dee2/code/volunteer_pipeline.sh SRR13165364 current disk space = 1516103086080 free memory = 1607712964 SRR13165364 SRAfilesize 991cae1060e40facf40e6777ec3cf91a SRR13165364.sra SRR13165364.sra file validated SRR13165364 is paired end SRR13165364 is conventional basespace SRR13165364 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165364_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.486 37.0 37.0 37.0 37.0 37.0 2 36.2105 37.0 37.0 37.0 37.0 37.0 3 36.518 37.0 37.0 37.0 37.0 37.0 4 36.5015 37.0 37.0 37.0 37.0 37.0 5 36.5735 37.0 37.0 37.0 37.0 37.0 6 36.5655 37.0 37.0 37.0 37.0 37.0 7 36.5045 37.0 37.0 37.0 37.0 37.0 8 36.531 37.0 37.0 37.0 37.0 37.0 9 36.477 37.0 37.0 37.0 37.0 37.0 10-14 36.570100000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.480399999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.5068 37.0 37.0 37.0 37.0 37.0 25-29 36.44879999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.4409 37.0 37.0 37.0 37.0 37.0 35-39 36.411500000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.4135 37.0 37.0 37.0 37.0 37.0 45-49 36.407599999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.389799999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.3538 37.0 37.0 37.0 37.0 37.0 60-64 36.3928 37.0 37.0 37.0 37.0 37.0 65-69 36.2801 37.0 37.0 37.0 37.0 37.0 70-74 36.311 37.0 37.0 37.0 37.0 37.0 75-79 36.2825 37.0 37.0 37.0 37.0 37.0 80-84 36.265499999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.239000000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.2353 37.0 37.0 37.0 37.0 37.0 95-99 36.193200000000004 37.0 37.0 37.0 37.0 37.0 100-104 36.136 37.0 37.0 37.0 37.0 37.0 105-109 36.176 37.0 37.0 37.0 37.0 37.0 110-114 36.143299999999996 37.0 37.0 37.0 37.0 37.0 115-119 36.1243 37.0 37.0 37.0 37.0 37.0 120-124 36.064499999999995 37.0 37.0 37.0 37.0 37.0 125-129 36.0136 37.0 37.0 37.0 37.0 37.0 130-134 36.036500000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.9333 37.0 37.0 37.0 37.0 37.0 140-144 35.8267 37.0 37.0 37.0 37.0 37.0 145-149 35.6156 37.0 37.0 37.0 37.0 37.0 150-151 35.44125 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 1.0 24 1.0 25 2.0 26 7.0 27 12.0 28 12.0 29 17.0 30 25.0 31 39.0 32 51.0 33 68.0 34 116.0 35 325.0 36 2890.0 37 432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.825 10.325 5.875 33.975 2 22.50125565042692 11.175288799598192 32.169763937719736 34.15369161225515 3 20.349999999999998 16.775000000000002 25.074999999999996 37.8 4 25.924999999999997 21.95 21.5 30.625000000000004 5 24.975 27.200000000000003 24.05 23.775 6 24.6 30.375000000000004 22.825 22.2 7 19.55 24.5 38.4 17.549999999999997 8 20.200000000000003 23.05 31.2 25.55 9 21.45 21.8 30.75 26.0 10-14 23.595 26.44 25.295 24.67 15-19 23.235 23.294999999999998 27.26 26.21 20-24 24.39 25.03 24.86 25.72 25-29 23.695 25.575 25.014999999999997 25.715 30-34 23.169999999999998 25.545 24.915000000000003 26.369999999999997 35-39 23.415 24.69 25.575 26.32 40-44 24.435000000000002 25.495 24.605 25.465 45-49 23.955000000000002 24.945 25.545 25.555 50-54 24.295 25.135 24.68 25.89 55-59 24.060000000000002 24.565 25.775 25.6 60-64 23.44 24.795 24.745 27.02 65-69 23.380000000000003 25.94 24.775 25.905 70-74 23.645 25.679999999999996 24.8 25.874999999999996 75-79 24.560000000000002 24.474999999999998 25.215 25.75 80-84 24.69 24.91 24.81 25.590000000000003 85-89 24.26 24.990000000000002 24.51 26.240000000000002 90-94 24.315 24.04 25.525 26.119999999999997 95-99 23.715 25.095 24.545 26.645000000000003 100-104 23.580000000000002 26.525 24.565 25.330000000000002 105-109 24.88 25.795 23.810000000000002 25.515 110-114 24.735 25.89 23.64 25.735000000000003 115-119 24.36 25.369999999999997 24.305 25.965 120-124 24.245 26.005 23.86 25.89 125-129 24.104999999999997 25.56 23.47 26.865 130-134 24.75 24.765 23.745 26.740000000000002 135-139 23.96 25.669999999999998 24.165 26.205000000000002 140-144 23.645 25.174999999999997 24.52 26.66 145-149 24.14 24.759999999999998 24.279999999999998 26.82 150-151 24.075 24.325 23.825 27.775 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.0 24 0.5 25 0.5 26 1.5 27 3.5 28 3.0 29 5.0 30 7.5 31 10.0 32 14.5 33 15.5 34 24.0 35 34.0 36 57.5 37 79.5 38 81.0 39 95.5 40 120.0 41 131.5 42 149.0 43 176.0 44 178.5 45 175.0 46 191.0 47 194.0 48 176.5 49 164.5 50 160.0 51 160.5 52 137.0 53 107.0 54 96.5 55 102.0 56 112.0 57 91.5 58 76.0 59 89.5 60 96.0 61 74.5 62 53.5 63 56.5 64 53.5 65 47.0 66 48.5 67 43.5 68 41.0 69 38.5 70 34.0 71 37.5 72 32.0 73 25.0 74 22.0 75 21.0 76 20.5 77 11.0 78 7.5 79 5.5 80 2.5 81 3.5 82 1.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.0 #Duplication Level Percentage of deduplicated Percentage of total 1 72.10714285714286 50.475 2 18.535714285714285 25.95 3 5.821428571428571 12.225 4 2.4285714285714284 6.800000000000001 5 0.6071428571428571 2.125 6 0.25 1.05 7 0.17857142857142858 0.8750000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.07142857142857142 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAATATCAGGCTCTTTGGTCCAGTCATGTACAGAATCAGGAAGGCCCCT 10 0.25 No Hit GCCTAAATCTCCAAATACCACACCAAGGCTTTGGAAAGCTAGTTGTAGCA 10 0.25 No Hit GAAAGATTTGGAATTTTTTGGTGGAGAAAGAACAGTTCTAGGTACGGAAC 7 0.17500000000000002 No Hit GCTCTCTTAAAATATCACTGATATTGATCCCAACATATCGAATTCCAGTC 7 0.17500000000000002 No Hit GTCCAACATATTCTCGAACAGGAGGAAATAACCCATCCATTCAGAGATTA 7 0.17500000000000002 No Hit GCCTCATATTGGTTGTCTTGGGACTCATCAAATAACTTATTTGAGGTTCT 7 0.17500000000000002 No Hit GCCTCTCATGCTCCCTCATTTCCACAAAAACTTGGCTCCTAGTAACGTCC 7 0.17500000000000002 No Hit GCCGAGGTAGTCAAGGCCTCCCTCGCTGAAGATCTGGGAGCCGGCCTTGA 6 0.15 No Hit GCTTGGAGATGGCGGAGCGTAGCTCGGAGGCGGTGACGACGTCGTCGAGG 6 0.15 No Hit GCAGGCAGAGTCGGTGTGGCTGTTGGCCTGGTAGTAGGCGTTCATAACGT 6 0.15 No Hit CCCTCACCAGTTCATTATAAAGCAATCCAATTCCAGGGGCTCTTACTGTA 6 0.15 No Hit CTGCTGGTGATGCGCAGAGGCGTAGAATGAGTTGTTCATCGGCTGCTGCT 6 0.15 No Hit GTCCTCTGCTGGCAATGCTTCGCCGACCCCTCTTCTGAAAGTGAAGTTTG 6 0.15 No Hit CTGGAGAAGGAGGACTGCAGGGGCCTCCCCAGCAGCTGCCTCCCAGATAG 6 0.15 No Hit GCACACTTGGTATATTTTAAGTTGTAAGAAGCAACAGGAGGGTGGTGCTT 5 0.125 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 5 0.125 No Hit GTTGTCGAAGTCGTACTTCCTTAGGCCCTGGCTGATGTACTCTTGGGAGC 5 0.125 No Hit CTGCTGCTTCAGATTCACTTGAGGTTCTGGTCAAACCAGCTAGTCATGTC 5 0.125 No Hit GTTGCATTTTGTTAGTAATATCCTGACCTAATCAGGAAACCGAATGATTT 5 0.125 No Hit GGCTCTAGTTGCAGGTGCAGCAGGAGCAGCCGCAGGCGGTGCCGCACTTG 5 0.125 No Hit GGGATGCTGACGACAGTGCGCCACTTGGCGAAGCGGGCGCCTTGCTGGTA 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit CTTGCCGTCGTTCTCCGCCGCGGACTCCTGCACCTCGAAGTGGCTCTTCT 5 0.125 No Hit GTCTTCTGATTCAACTTCCCGACGTGCTTGCAAAATACCTGAAATCTTGC 5 0.125 No Hit GTGGTATTCTACTCAGCTCTCCAAATCGTAAACATTCTTATAACAGCAGA 5 0.125 No Hit GTCCTTGGTGACCCCGCTTACTTGAAGTGCTTTATCAGGAGAGCCTGTAT 5 0.125 No Hit GTAGCCATGAAAGCCAGTCCAATGCCTGTGTTGCCACTGGTTGGTTCAAT 5 0.125 No Hit ACATGTTGCAGCCGTTGCAGCCGCTGCCGCACTTGCAGGATGACCCGCAG 5 0.125 No Hit CCGTAATGTCCTTCCTTTGGCGAGGATCAGATGTTGGTAAATACATTATT 5 0.125 No Hit CTACTGTACAAGAATAGGTTGTGCAATGGTTCTACTAACCTTTGCCAGGC 5 0.125 No Hit GTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.1375 0.0 0.0 0.0 0.0 68-69 0.16249999999999998 0.0 0.0 0.0 0.0 70-71 0.25 0.0 0.0 0.0 0.0 72-73 0.2875 0.0 0.0 0.0 0.0 74-75 0.3875 0.0 0.0 0.0 0.0 76-77 0.42500000000000004 0.0 0.0 0.0 0.0 78-79 0.6375 0.0 0.0 0.0 0.0 80-81 0.7125 0.0 0.0 0.0 0.0 82-83 0.8125 0.0 0.0 0.0 0.0 84-85 1.0 0.0 0.0 0.0 0.0 86-87 1.3125 0.0 0.0 0.0 0.0 88-89 1.6375000000000002 0.0 0.0 0.0 0.0 90-91 1.8875 0.0 0.0 0.0 0.0 92-93 2.1125 0.0 0.0 0.0 0.0 94-95 2.45 0.0 0.0 0.0 0.0 96-97 2.7375 0.0 0.0 0.0 0.0 98-99 3.35 0.0 0.0 0.0 0.0 100-101 3.95 0.0 0.0 0.0 0.0 102-103 4.6875 0.0 0.0 0.0 0.0 104-105 5.4125 0.0 0.0 0.0 0.0 106-107 6.112500000000001 0.0 0.0 0.0 0.0 108-109 6.65 0.0 0.0 0.0 0.0 110-111 7.375 0.0 0.0 0.0 0.0 112-113 8.025 0.0 0.0 0.0 0.0 114-115 9.024999999999999 0.0 0.0 0.0 0.0 116-117 9.65 0.0 0.0 0.0 0.0 118-119 10.337499999999999 0.0 0.0 0.0 0.0 120-121 11.05 0.0 0.0 0.0 0.0 122-123 11.5625 0.0 0.0 0.0 0.0 124-125 12.3875 0.0 0.0 0.0 0.0 126-127 13.4125 0.0 0.0 0.0 0.0 128-129 14.1 0.0 0.0 0.0 0.0 130-131 15.0375 0.0 0.0 0.0 0.0 132-133 15.9875 0.0 0.0 0.0 0.0 134-135 16.725 0.0 0.0 0.0 0.0 136-137 17.4 0.0 0.0 0.0 0.0 138-139 18.012500000000003 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTGGT 10 0.006830828 145.0 8 TCTACTC 10 0.006830828 145.0 8 CTTGGTC 10 0.006830828 145.0 9 TTCTACT 10 0.006830828 145.0 7 TCTCCTT 10 0.006830828 145.0 5 GTGGTAT 10 0.006830828 145.0 1 GCATTTT 10 0.006830828 145.0 4 CTACTCA 10 0.006830828 145.0 9 AAAAAAA 65 0.0076375785 13.384615 75-79 >>END_MODULE SRR13165364 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165364_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0765 37.0 37.0 37.0 37.0 37.0 2 36.18 37.0 37.0 37.0 37.0 37.0 3 36.095 37.0 37.0 37.0 37.0 37.0 4 36.1095 37.0 37.0 37.0 37.0 37.0 5 36.214 37.0 37.0 37.0 37.0 37.0 6 36.1385 37.0 37.0 37.0 37.0 37.0 7 36.248 37.0 37.0 37.0 37.0 37.0 8 36.2595 37.0 37.0 37.0 37.0 37.0 9 36.2505 37.0 37.0 37.0 37.0 37.0 10-14 36.2262 37.0 37.0 37.0 37.0 37.0 15-19 36.166199999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.086650000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.103449999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.01085 37.0 37.0 37.0 37.0 37.0 35-39 36.014250000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.00255 37.0 37.0 37.0 37.0 37.0 45-49 35.95405 37.0 37.0 37.0 37.0 37.0 50-54 35.904250000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.86985 37.0 37.0 37.0 37.0 37.0 60-64 35.89334999999999 37.0 37.0 37.0 37.0 37.0 65-69 35.81415 37.0 37.0 37.0 37.0 37.0 70-74 35.77935000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.811350000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.83555 37.0 37.0 37.0 37.0 37.0 85-89 35.70935 37.0 37.0 37.0 37.0 37.0 90-94 35.69965 37.0 37.0 37.0 37.0 37.0 95-99 35.73135 37.0 37.0 37.0 37.0 37.0 100-104 35.62885000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.67115 37.0 37.0 37.0 37.0 37.0 110-114 35.593650000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.560449999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.463049999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.42665000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.24305 37.0 37.0 37.0 32.2 37.0 135-139 35.13445 37.0 37.0 37.0 29.8 37.0 140-144 35.021049999999995 37.0 37.0 37.0 25.0 37.0 145-149 34.68085 37.0 37.0 37.0 25.0 37.0 150-151 34.49575 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 2.0 14 8.0 15 7.0 16 3.0 17 2.0 18 3.0 19 7.0 20 6.0 21 8.0 22 4.0 23 4.0 24 11.0 25 8.0 26 5.0 27 16.0 28 11.0 29 17.0 30 22.0 31 33.0 32 73.0 33 107.0 34 188.0 35 584.0 36 2638.0 37 231.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.310776942355886 21.278195488721803 8.847117794486216 25.563909774436087 2 31.624999999999996 21.85 25.424999999999997 21.099999999999998 3 22.900000000000002 24.75 29.65 22.7 4 28.15 28.625 20.875 22.35 5 27.224999999999998 33.25 19.1 20.424999999999997 6 23.625 33.675 20.5 22.2 7 22.575 19.975 33.45 24.0 8 24.125 24.125 24.45 27.3 9 24.825 22.05 24.925 28.199999999999996 10-14 25.81 26.224999999999998 22.875 25.09 15-19 26.435 24.795 23.805 24.965 20-24 26.046511627906977 25.566391597899475 24.17604401100275 24.2110527631908 25-29 26.061515378844714 24.93623405851463 24.271067766941734 24.731182795698924 30-34 26.121530382595648 25.881470367591895 23.900975243810954 24.0960240060015 35-39 25.701425356339087 25.676419104776194 24.001000250062514 24.621155288822205 40-44 26.03150787696924 26.116529132283073 23.61090272568142 24.241060265066267 45-49 25.68642160540135 25.056264066016503 24.63115778944736 24.626156539134783 50-54 27.181795448862218 23.980995248812203 24.701175293823454 24.136034008502126 55-59 26.941735433858465 24.866216554138536 23.540885221305327 24.651162790697676 60-64 26.78169542385596 24.351087771942986 24.28607151787947 24.58114528632158 65-69 26.736684171042764 25.656414103525883 23.465866466616657 24.141035258814703 70-74 26.89172293073268 25.14128532133033 23.735933983495876 24.23105776444111 75-79 26.426606651662915 24.946236559139784 24.72118029507377 23.905976494123532 80-84 26.47161790447612 25.346336584146034 24.466116529132282 23.71592898224556 85-89 26.846711677919483 25.43635908977244 23.940985246311577 23.775943985996502 90-94 26.241560390097522 25.271317829457363 24.381095273818453 24.10602650662666 95-99 26.696674168542135 25.87146786696674 23.950987746936732 23.48087021755439 100-104 26.71167791947987 24.746186546636658 24.616154038509627 23.925981495373843 105-109 27.611902975743934 25.561390347586897 22.99074768692173 23.835958989747436 110-114 27.71692923230808 26.021505376344084 23.05076269067267 23.210802700675167 115-119 28.537134283570893 25.171292823205803 23.460865216304075 22.83070767691923 120-124 28.527131782945737 25.541385346336583 22.61565391347837 23.315828957239308 125-129 28.912228057014254 25.921480370092524 22.34558639659915 22.820705176294073 130-134 29.327331832958244 24.88122030507627 23.13078269567392 22.660665166291576 135-139 30.207551887971995 25.43635908977244 22.75068767191798 21.605401350337583 140-144 30.72268067016754 26.446611652913226 21.47536884221055 21.355338834708675 145-149 31.34783695923981 24.561140285071268 22.34558639659915 21.745436359089773 150-151 32.52063015753939 24.956239059764943 21.417854463615903 21.10527631907977 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 1.0 10 0.5 11 1.0 12 1.5 13 1.0 14 0.5 15 0.5 16 0.5 17 0.5 18 1.5 19 1.5 20 1.5 21 1.5 22 0.5 23 1.0 24 1.0 25 2.0 26 1.5 27 0.0 28 6.0 29 13.0 30 17.0 31 15.5 32 10.5 33 14.5 34 26.0 35 40.5 36 47.0 37 52.5 38 74.0 39 84.0 40 94.0 41 137.5 42 158.0 43 161.0 44 186.5 45 187.5 46 175.0 47 167.5 48 155.5 49 155.0 50 151.0 51 144.5 52 127.0 53 118.0 54 124.5 55 103.5 56 87.0 57 105.0 58 106.0 59 86.0 60 77.5 61 76.5 62 61.0 63 53.0 64 60.5 65 56.0 66 60.5 67 49.0 68 35.5 69 39.5 70 41.5 71 36.0 72 34.5 73 36.5 74 29.5 75 24.0 76 14.0 77 10.5 78 10.0 79 4.5 80 4.0 81 3.0 82 1.0 83 2.0 84 2.0 85 0.5 86 0.0 87 0.5 88 1.0 89 1.0 90 0.5 91 1.5 92 2.0 93 1.0 94 1.0 95 1.5 96 1.0 97 0.0 98 1.0 99 3.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.025 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.025 135-139 0.025 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.175 #Duplication Level Percentage of deduplicated Percentage of total 1 72.96045600285001 51.2 2 17.91948699679373 25.15 3 5.7356608478802995 12.075 4 2.1375133594584965 6.0 5 0.6412540078375489 2.25 6 0.21375133594584966 0.8999999999999999 7 0.21375133594584966 1.05 8 0.035625222657641606 0.2 9 0.0 0.0 >10 0.14250089063056642 1.175 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 14 0.35000000000000003 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 13 0.325 No Hit ATCCAATCACGATGGGTGCACATGACATCGAGGGTGCCTTAGCCTGGAAA 10 0.25 No Hit TACTGCTGCCAATTGGTCTGAACTCATGGAGACCATTTCAAGTTCAACAA 10 0.25 No Hit CACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTT 8 0.2 No Hit ATGCAAGCTGGTCTGGTCTGTGATTTGTGTTATATACTAAATCTAGTATA 7 0.17500000000000002 No Hit ATCGTCTCCGTTCCTCGCCGATTGAAGCACCGACGCCCCCCAATTCTTGC 7 0.17500000000000002 No Hit CACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAG 7 0.17500000000000002 No Hit CGTGATAACGAGTTTTTTGCAACTGCTGGTGTAAATAAGAAGATAAAAGT 7 0.17500000000000002 No Hit GGAACGGGCCCGAACTTAAAGTACTATGCAAGTGCTGATGGAGTAAACGT 7 0.17500000000000002 No Hit GCAGGCTTGCTGACTGCTGCTAGCATGAGTTAGTGTTTTCCCTTTTTCTA 7 0.17500000000000002 No Hit GCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTT 6 0.15 No Hit CTGTATTTGATAATGTACATATGGAACTATGGAACCAAGTTGAAGTATGA 6 0.15 No Hit AGTGATGGCAGAAGAAAAGTTAGTGCTAAGCTTCAGAAAACAATAGATAA 6 0.15 No Hit TGAGGGTTTGCAGCAGTGGCAAGGGGACGCCGTTGATGCCGAATAGGACT 6 0.15 No Hit CGGTGAAGGTGCCGCCGAACTCGGCGTCGATGGAGGAGGCGCGGCACCGC 6 0.15 No Hit AAGAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGC 6 0.15 No Hit GTTGATATGGCTGAGGGCAGGAAAAGATTAGGATCCAACATAGCAGTTCA 5 0.125 No Hit AGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAG 5 0.125 No Hit TGGAAGGTAAATTGAGGGAATAAAATTGTTGGCTACTCTTGTTTTCTTTT 5 0.125 No Hit AGAAACATCCTTAACTGAGCTCCTCACTCACTCACTGCAGCTAGCCTCTT 5 0.125 No Hit GGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAG 5 0.125 No Hit GGAACTTTGGCGTTTGTGTTCCTGCCGCACAGAAGGCGTCAGATCCCATC 5 0.125 No Hit ATGGAGGAATTGCTGCAGTTAATTGAGAAGGAAGATATACCTGCACCTGC 5 0.125 No Hit GTACAACTGAAACCCCGCGACACGAGAAGTACGTGCCATTCAAACTCCAG 5 0.125 No Hit GCCGGGGGAACGGGTACCAGAACCTCTTCGACGCCCTCGTCGACACGTTC 5 0.125 No Hit GTGTCCGCGTCCGGCAGCCCGTGGTACGGCTCCGACCGCGTGCTCTACCT 5 0.125 No Hit AGCTCAGGGAGATCGGCTTCATCGTCTACTCCATCGGCAAGCCGCTCGAC 5 0.125 No Hit GTTCTATCCTCCAACTCAGGGGTTGGTCACTTTGTGAAGATTTTCCCTGG 5 0.125 No Hit CGCTTGTCGAGGGCGGCGGGATGGGGGAGGCTGCGACGCCGGCGATCGCC 5 0.125 No Hit GCTCTGGGAACTTTTAGAGTCAACAGATTGAGAAGAGCATAAGTATTTAA 5 0.125 No Hit CCCAGAATAGCTTTCTTTTCAAGGACAAAATTGTCCTTGATGTTGGTGCT 5 0.125 No Hit CCACACACTGCACTTTCCTCATCTCCCCGAAAGCAGGAGCTAGAGGAGGA 5 0.125 No Hit GTTGAAGAATCAGCGGATGAGTTGTGGTTAGGGGTGAAATGCCACTCGAA 5 0.125 No Hit CAAGGTTGACAAGGGTCTTGTGCCACTCGTTGGTTCCAACGACGAGTCAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.037500000000000006 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.16249999999999998 0.0 0.0 0.0 0.0 68-69 0.1875 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.3125 0.0 0.0 0.0 0.0 74-75 0.4125 0.0 0.0 0.0 0.0 76-77 0.44999999999999996 0.0 0.0 0.0 0.0 78-79 0.6625 0.0 0.0 0.0 0.0 80-81 0.7375 0.0 0.0 0.0 0.0 82-83 0.8374999999999999 0.0 0.0 0.0 0.0 84-85 1.025 0.0 0.0 0.0 0.0 86-87 1.3250000000000002 0.0 0.0 0.0 0.0 88-89 1.6375000000000002 0.0 0.0 0.0 0.0 90-91 1.8875 0.0 0.0 0.0 0.0 92-93 2.1375 0.0 0.0 0.0 0.0 94-95 2.4749999999999996 0.0 0.0 0.0 0.0 96-97 2.7625 0.0 0.0 0.0 0.0 98-99 3.3375 0.0 0.0 0.0 0.0 100-101 3.9125 0.0 0.0 0.0 0.0 102-103 4.625 0.0 0.0 0.0 0.0 104-105 5.3375 0.0 0.0 0.0 0.0 106-107 6.012499999999999 0.0 0.0 0.0 0.0 108-109 6.550000000000001 0.0 0.0 0.0 0.0 110-111 7.275 0.0 0.0 0.0 0.0 112-113 7.9125000000000005 0.0 0.0 0.0 0.0 114-115 8.875 0.0 0.0 0.0 0.0 116-117 9.5 0.0 0.0 0.0 0.0 118-119 10.175 0.0 0.0 0.0 0.0 120-121 10.875 0.0 0.0 0.0 0.0 122-123 11.3875 0.0 0.0 0.0 0.0 124-125 12.2 0.0 0.0 0.0 0.0 126-127 13.225 0.0 0.0 0.0 0.0 128-129 13.9 0.0 0.0 0.0 0.0 130-131 14.8375 0.0 0.0 0.0 0.0 132-133 15.75 0.0 0.0 0.0 0.0 134-135 16.5 0.0 0.0 0.0 0.0 136-137 17.1625 0.0 0.0 0.0 0.0 138-139 17.7875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTTT 10 0.006830828 145.0 9 TTCAAAG 10 0.006830828 145.0 6 GAACTTT 10 0.006830828 145.0 2 TGCTTAA 10 0.006830828 145.0 3 TATTCAA 10 0.006830828 145.0 4 GCTGAGG 10 0.006830828 145.0 145 TTTGGCG 10 0.006830828 145.0 6 TTGGCGT 10 0.006830828 145.0 7 TGGCGTT 10 0.006830828 145.0 8 CTTTGGC 10 0.006830828 145.0 5 AAAGTAC 10 0.006830828 145.0 9 >>END_MODULE Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386517 spots for SRR13165364.sra Written 1386517 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra Read 1386514 spots for SRR13165364.sra Written 1386514 spots for SRR13165364.sra SRR ids: ['SRR13165364.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6mgm02rc SRR13165364.sra spots: 27730283 blocks: [[1, 1386514], [1386515, 2773028], [2773029, 4159542], [4159543, 5546056], [5546057, 6932570], [6932571, 8319084], [8319085, 9705598], [9705599, 11092112], [11092113, 12478626], [12478627, 13865140], [13865141, 15251654], [15251655, 16638168], [16638169, 18024682], [18024683, 19411196], [19411197, 20797710], [20797711, 22184224], [22184225, 23570738], [23570739, 24957252], [24957253, 26343766], [26343767, 27730283]] SRR13165364 file size 9402262 SRR13165364 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165364 SRR13165364_1.fastq SRR13165364_2.fastq Input file: SRR13165364_1.fastq Paired file: SRR13165364_2.fastq trimmed: SRR13165364-trimmed-pair1.fastq, SRR13165364-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 02:16:57 2024 >> started Thu Dec 12 02:17:42 2024 >> done (45.007s) 27730283 read pairs processed; of these: 502 ( 0.00%) short read pairs filtered out after trimming by size control 30932 ( 0.11%) empty read pairs filtered out after trimming by size control 27698849 (99.89%) read pairs available; of these: 6105310 (22.04%) trimmed read pairs available after processing 21593539 (77.96%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 29 0.00% 19 22 0.00% 20 38 0.00% 21 38 0.00% 22 52 0.00% 23 53 0.00% 24 55 0.00% 25 102 0.00% 26 100 0.00% 27 123 0.00% 28 111 0.00% 29 109 0.00% 30 123 0.00% 31 173 0.00% 32 181 0.00% 33 183 0.00% 34 186 0.00% 35 211 0.00% 36 212 0.00% 37 224 0.00% 38 281 0.00% 39 283 0.00% 40 299 0.00% 41 350 0.00% 42 431 0.00% 43 432 0.00% 44 437 0.00% 45 438 0.00% 46 534 0.00% 47 598 0.00% 48 681 0.00% 49 781 0.00% 50 825 0.00% 51 942 0.00% 52 1144 0.00% 53 1066 0.00% 54 1259 0.00% 55 1319 0.00% 56 1544 0.01% 57 1583 0.01% 58 1963 0.01% 59 2173 0.01% 60 2524 0.01% 61 2817 0.01% 62 3167 0.01% 63 3825 0.01% 64 3920 0.01% 65 4233 0.02% 66 4567 0.02% 67 4899 0.02% 68 5844 0.02% 69 6122 0.02% 70 7139 0.03% 71 8030 0.03% 72 9335 0.03% 73 10487 0.04% 74 11457 0.04% 75 12537 0.05% 76 13764 0.05% 77 14943 0.05% 78 16062 0.06% 79 17613 0.06% 80 19316 0.07% 81 21731 0.08% 82 23789 0.09% 83 26347 0.10% 84 29333 0.11% 85 31164 0.11% 86 33009 0.12% 87 34645 0.13% 88 36352 0.13% 89 37925 0.14% 90 40466 0.15% 91 42553 0.15% 92 45950 0.17% 93 48925 0.18% 94 52851 0.19% 95 55057 0.20% 96 57590 0.21% 97 59591 0.22% 98 60361 0.22% 99 63373 0.23% 100 65321 0.24% 101 66590 0.24% 102 69284 0.25% 103 71131 0.26% 104 74576 0.27% 105 75340 0.27% 106 78571 0.28% 107 78845 0.28% 108 80262 0.29% 109 82486 0.30% 110 83042 0.30% 111 84863 0.31% 112 86781 0.31% 113 87037 0.31% 114 91486 0.33% 115 92534 0.33% 116 95073 0.34% 117 95031 0.34% 118 96242 0.35% 119 96296 0.35% 120 98098 0.35% 121 98770 0.36% 122 99864 0.36% 123 101251 0.37% 124 103884 0.38% 125 105713 0.38% 126 105628 0.38% 127 107308 0.39% 128 107268 0.39% 129 108510 0.39% 130 107848 0.39% 131 108330 0.39% 132 109490 0.40% 133 111062 0.40% 134 111465 0.40% 135 112130 0.40% 136 112982 0.41% 137 112651 0.41% 138 112073 0.40% 139 113888 0.41% 140 114762 0.41% 141 114113 0.41% 142 115840 0.42% 143 115543 0.42% 144 116520 0.42% 145 118231 0.43% 146 119811 0.43% 147 124588 0.45% 148 119442 0.43% 149 122199 0.44% 150 119956 0.43% 151 21593539 77.96% 27698849 reads passed initial QC criterion=sequence-density sequence-density=0.93 sequence-density-rank=1 fanout-score=2.28 fanout-score-rank=27 prefix-density=0.94 prefix-fanout=2.3 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.12 sequence-density-rank=13 fanout-score=18.40 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=6.8 sequence=GCAGCTGCAGCT criterion=sequence-density sequence-density=0.47 sequence-density-rank=1 fanout-score=2.90 fanout-score-rank=30 prefix-density=0.56 prefix-fanout=2.5 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.11 sequence-density-rank=16 fanout-score=80.34 fanout-score-rank=1 prefix-density=0.64 prefix-fanout=14.1 sequence=GCCGCCGCCGCCA SRR13165364 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 02:18:54 Started mapping on | Dec 12 02:18:54 Finished on | Dec 12 02:22:43 Mapping speed, Million of reads per hour | 435.44 Number of input reads | 27698849 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 21248838 Uniquely mapped reads % | 76.71% Average mapped length | 289.40 Number of splices: Total | 22305576 Number of splices: Annotated (sjdb) | 20948663 Number of splices: GT/AG | 21990403 Number of splices: GC/AG | 268763 Number of splices: AT/AC | 10088 Number of splices: Non-canonical | 36322 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.54 Insertion rate per base | 0.01% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 361767 % of reads mapped to multiple loci | 1.31% Number of reads mapped to too many loci | 102650 % of reads mapped to too many loci | 0.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 20.25% % of reads unmapped: other | 1.36% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6088545 6088545 6088545 N_multimapping 361767 361767 361767 N_noFeature 892920 20642485 1067616 N_ambiguous 572041 4183 142225 UnstrandedReadsAssigned:19783877 PositiveStrandReadsAssigned:602170 NegativeStrandReadsAssigned:20038997 Dataset is classified negative stranded MeadianReadLen=147 20thPercentileLength=142 echo kmer=137 SRR13165364 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165364-trimmed-pair1.fastq SRR13165364-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,698,849 reads, 24,244,154 reads pseudoaligned [quant] estimated average fragment length: 227.553 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,217 rounds 52973 SRR13165364.ke.tsv 35125 SRR13165364.se.tsv 88098 total ==> SRR13165364.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 709.865 0 0 PNS24247 1044 817.447 73.8105 5.24864 PNS24249 1928 1701.45 224.85 7.68179 PNS24246 1044 817.447 73.8105 5.24864 PNS24248 1044 817.447 73.8105 5.24864 PNS24244 1471 1244.45 96.7186 4.51775 PNS24243 293 117.526 0 0 KQK14069 1603 1376.45 1506.23 63.6093 KQK14071 474 263.669 36.356 8.01505 ==> SRR13165364.se.tsv <== BRADI_1g14170v3 1394 BRADI_1g53295v3 175 BRADI_1g59795v3 532 BRADI_1g07683v3 0 BRADI_1g00485v3 3 BRADI_1g20270v3 463 BRADI_1g74790v3 601 BRADI_1g09890v3 1 BRADI_1g77505v3 342 BRADI_1g48960v3 0 SRR13165364 completed mapping pipeline successfully