Starting /dee2/code/volunteer_pipeline.sh SRR13165365 current disk space = 1541905829888 free memory = 1607481804 SRR13165365 SRAfilesize 579162829855a1b54aef267d0b0484cb SRR13165365.sra SRR13165365.sra file validated SRR13165365 is paired end SRR13165365 is conventional basespace SRR13165365 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165365_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5155 37.0 37.0 37.0 37.0 37.0 2 36.26925 37.0 37.0 37.0 37.0 37.0 3 36.4285 37.0 37.0 37.0 37.0 37.0 4 36.5745 37.0 37.0 37.0 37.0 37.0 5 36.5515 37.0 37.0 37.0 37.0 37.0 6 36.5255 37.0 37.0 37.0 37.0 37.0 7 36.4535 37.0 37.0 37.0 37.0 37.0 8 36.4995 37.0 37.0 37.0 37.0 37.0 9 36.508 37.0 37.0 37.0 37.0 37.0 10-14 36.5359 37.0 37.0 37.0 37.0 37.0 15-19 36.5227 37.0 37.0 37.0 37.0 37.0 20-24 36.5162 37.0 37.0 37.0 37.0 37.0 25-29 36.4694 37.0 37.0 37.0 37.0 37.0 30-34 36.4627 37.0 37.0 37.0 37.0 37.0 35-39 36.3714 37.0 37.0 37.0 37.0 37.0 40-44 36.3507 37.0 37.0 37.0 37.0 37.0 45-49 36.1413 37.0 37.0 37.0 37.0 37.0 50-54 36.278299999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.064499999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.013999999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.8895 37.0 37.0 37.0 37.0 37.0 70-74 36.0788 37.0 37.0 37.0 37.0 37.0 75-79 36.2164 37.0 37.0 37.0 37.0 37.0 80-84 36.244299999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.247 37.0 37.0 37.0 37.0 37.0 90-94 36.207499999999996 37.0 37.0 37.0 37.0 37.0 95-99 36.218199999999996 37.0 37.0 37.0 37.0 37.0 100-104 36.133900000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.1669 37.0 37.0 37.0 37.0 37.0 110-114 36.168 37.0 37.0 37.0 37.0 37.0 115-119 36.0757 37.0 37.0 37.0 37.0 37.0 120-124 36.0356 37.0 37.0 37.0 37.0 37.0 125-129 35.933499999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.900099999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.823699999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.7068 37.0 37.0 37.0 37.0 37.0 145-149 35.4524 37.0 37.0 37.0 37.0 37.0 150-151 35.110749999999996 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 1.0 25 6.0 26 5.0 27 6.0 28 17.0 29 26.0 30 38.0 31 30.0 32 67.0 33 80.0 34 203.0 35 340.0 36 2716.0 37 463.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.349999999999994 10.174999999999999 5.7 31.775 2 25.371255977850492 11.880191291215706 29.599798640825576 33.14875409010823 3 21.05 13.375 27.975 37.6 4 25.6 18.875 22.675 32.85 5 29.725 23.549999999999997 23.175 23.549999999999997 6 28.625 26.525 20.65 24.2 7 19.400000000000002 26.5 36.925000000000004 17.175 8 21.6 23.7 28.025 26.674999999999997 9 23.25 20.0 30.725 26.025 10-14 23.22 26.05 24.81 25.919999999999998 15-19 23.580000000000002 24.145 25.14 27.134999999999998 20-24 24.38 24.349999999999998 25.235000000000003 26.035000000000004 25-29 23.73 24.82 24.705 26.745 30-34 23.905 24.075 24.27 27.750000000000004 35-39 23.41 24.58 25.1 26.91 40-44 24.595 24.38 25.03 25.995 45-49 23.655 23.665 25.19 27.49 50-54 24.115000000000002 23.175 24.64 28.07 55-59 24.0 23.585 25.235000000000003 27.18 60-64 24.3 23.905 25.555 26.240000000000002 65-69 24.325 24.695 24.605 26.375 70-74 26.3 23.86 23.724999999999998 26.115 75-79 26.369999999999997 23.810000000000002 23.880000000000003 25.94 80-84 26.045 23.235 24.185000000000002 26.534999999999997 85-89 26.790000000000003 23.78 23.51 25.919999999999998 90-94 26.700000000000003 23.87 23.36 26.07 95-99 25.715 22.85 24.05 27.384999999999998 100-104 26.625 23.150000000000002 23.47 26.755000000000003 105-109 27.3 22.665 23.625 26.41 110-114 25.965 24.2 23.69 26.145000000000003 115-119 25.935000000000002 23.62 24.060000000000002 26.384999999999998 120-124 26.505000000000003 23.919999999999998 23.06 26.515 125-129 26.39 22.8 23.3 27.51 130-134 26.545 23.89 23.200000000000003 26.365 135-139 26.85 22.71 23.23 27.21 140-144 27.0 23.369999999999997 23.505000000000003 26.125 145-149 26.265 23.53 23.285 26.919999999999998 150-151 26.3125 23.799999999999997 23.599999999999998 26.2875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 1.0 25 2.0 26 1.5 27 2.5 28 2.0 29 2.0 30 3.0 31 5.0 32 10.0 33 10.5 34 11.0 35 19.0 36 35.5 37 41.0 38 51.0 39 84.5 40 105.0 41 125.0 42 140.5 43 156.0 44 166.0 45 155.0 46 162.5 47 170.0 48 157.5 49 143.5 50 156.5 51 161.5 52 161.5 53 148.5 54 125.0 55 140.0 56 135.0 57 99.5 58 89.0 59 88.5 60 86.5 61 85.0 62 66.0 63 55.0 64 65.5 65 80.0 66 84.5 67 74.5 68 56.5 69 41.0 70 29.5 71 26.5 72 28.0 73 35.0 74 32.0 75 21.5 76 19.0 77 13.5 78 9.5 79 12.0 80 8.5 81 1.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.675 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.80000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 71.01769911504425 48.15 2 19.358407079646017 26.25 3 5.678466076696165 11.55 4 2.1386430678466075 5.800000000000001 5 0.995575221238938 3.375 6 0.5162241887905604 2.1 7 0.11061946902654868 0.525 8 0.11061946902654868 0.6 9 0.0 0.0 >10 0.07374631268436578 1.6500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATTCGTATCTCGTAT 43 1.075 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATTCGTATCGCGTAT 23 0.575 TruSeq Adapter, Index 2 (97% over 37bp) GAGAGAGAGAGAACAAATATTGGAGCTAAGAAATTAAAAAGGACACAGTC 8 0.2 No Hit CATGACATGAGATTATTACGTCCATAAGGAAGCGAGCAAATACATATAAT 8 0.2 No Hit ACCTTGTCGAACAGCTTCTGCTGGTACTGGTCGAGCGCGAGCATCTCCAG 8 0.2 No Hit AGCCAATTAAACTTTCAGGGTTTTTTGCACGACCACGTACGTGACAGACA 7 0.17500000000000002 No Hit CCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTGGA 7 0.17500000000000002 No Hit GTCCGGGTCGAGCCGTTCGAGCGCGGCGATCTCCTCGTCGAGCTTGTCCT 7 0.17500000000000002 No Hit CCGTCGAGTTATCATGAATCATCGGATCAGCGAGCAAAGCCCGCGTCAGC 6 0.15 No Hit CCTTGTAAGTAGTAACCAAATCAGGGTGTCATATATTTACTGTTTGGGGT 6 0.15 No Hit CAGCCTTTTATCTAATAAATGCGCCCCTCCCAGAAGTCGGGGTTTGTTGC 6 0.15 No Hit GTCTGGGTACATCTTCCCGCAAGTGCAGTTTGAGCCACAGTTGCAGCTTG 6 0.15 No Hit CTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCAT 6 0.15 No Hit GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG 6 0.15 No Hit CCCGGGCGACGACGGCGGGGGCGCAGCTCCTCGCCGCGCCCTGCTGCTGC 6 0.15 No Hit CTTGTATACACTGAAATATTGCAGAGTGCTCAGAATCAGCAGGAAGTATT 6 0.15 No Hit GCGGAGAAGGAATAGGAGGAGGTGTGCTTAGTGGTAGCATACGCCGCCGT 6 0.15 No Hit CAATAATCAGGTACTACCACACGTTCGGCAAATGAGTAACACACCTCACA 6 0.15 No Hit CTACCGTCTATGTCAAGAACATGTGACTGACCACTAGATGAGCCTTCGGA 6 0.15 No Hit CTCCGAATGCTCCAAGCTGTTCTCAGCAGTGGAATCAGCGTCGTACACGG 6 0.15 No Hit GTCTGGACCTCGGCTTCAGGGTACATGTTGCAGCCGTTGCAGCCGCTGCC 6 0.15 No Hit ACCGACGTAGAAATTAATTCATGGCAAAAACAATCGCGACCGATCGATCT 6 0.15 No Hit GTAACATCTCGAGATCTCCACCAAAACGACAGTCAGGTAAACTACCACCA 5 0.125 No Hit GCCATGCCCATCTGCTCGTCCTCCGAGTCAGTCTCGTACTCAGACTCTTC 5 0.125 No Hit CAGCTCATGGAACCCCAACAATCTCAAACATAGTAGCTTACATGGCAAGG 5 0.125 No Hit GCCAGAAGGAACCCTTTGCACCGTCCGCTGGAAATCTGGCTTGAAGCGCT 5 0.125 No Hit TGCTCCTCCACGTTCTTGTCGTACGCCGACGCGTGCACGGCCCTCCTCGG 5 0.125 No Hit CCAGCCTCCATCTCACAGCATAAGTAATCATCAAATCCACCCATTGGTCC 5 0.125 No Hit GTCGCCTCTCGTAGTGCTTGTTGAGTAAAGAACGCTACTACGAAGTAACC 5 0.125 No Hit GCTACTCGCAAAAGATGAAACTGACAAATCACTCTTTTGAACTTTTGGAA 5 0.125 No Hit CTCTGCTTTTGCTAGGCGTATTTCAACCTTTGTGGATAATACAAAATATT 5 0.125 No Hit GGCGGAACACACACAAACCCAGGAGGCCTAGGATCAACTAGGAAGACAAA 5 0.125 No Hit TGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCT 5 0.125 No Hit GGTGACATTTGTAACATTTCTGGTGAGTCGTGACATTTGTAACATTTCCT 5 0.125 No Hit CCGGCGTACTTGTTCTTGCAGTAGGACGGGACCATGTTGGCGATGTTGCG 5 0.125 No Hit GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCC 5 0.125 No Hit GCTGTAGTTTGAGTTGAAGCTGCTTGTAATCGCCAAGCAGAGATTTCTGC 5 0.125 No Hit ACCATGATTTAGCTTGTAGCTGATCCTGTTTCCTGGTCCAATCCTTTGTT 5 0.125 No Hit CTCCTTGCTCATACGGTCAGCAATACCCGGGAACATGGTTGAGCCACCAC 5 0.125 No Hit GGCTCAAGCTCCTTTTCGTAATCGTCTTCAGTGTATTCCAGCTTCTCAAA 5 0.125 No Hit CCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAG 5 0.125 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 5 0.125 No Hit ACTCACTCGAAGCTCCCGATCAAAAACACAAACAACACGACGACTGACAC 5 0.125 No Hit GGTTTATTGACGACTCCGTGATTAACTCATTAGTCATATGTATATATAAC 5 0.125 No Hit AATAGTTTCTTGGAACCACCAAAGCTAGTTTACCCACTTCATCTGGCGTG 5 0.125 No Hit CCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTACAATTCGGACGGCACG 5 0.125 No Hit CCTGAGTGTTCTGGAAGTAGTGCCTCCACAGGGGCCTGATCTTGTCCTGG 5 0.125 No Hit CCCAGTTGAATCTCGAAATACATACATGAGCAAGCATATGACTCACAGAG 5 0.125 No Hit GCTGGATCTTTCTCAATATGCTTTCTGTACTCATCCAGAGTTGTGGCACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.0625 0.0 0.0 0.0 0.0 58-59 0.0875 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1875 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.38749999999999996 0.0 0.0 0.0 0.0 78-79 0.5625 0.0 0.0 0.0 0.0 80-81 0.7125 0.0 0.0 0.0 0.0 82-83 1.075 0.0 0.0 0.0 0.0 84-85 1.4125 0.0 0.0 0.0 0.0 86-87 1.5875 0.0 0.0 0.0 0.0 88-89 1.7625000000000002 0.0 0.0 0.0 0.0 90-91 2.05 0.0 0.0 0.0 0.0 92-93 2.2750000000000004 0.0 0.0 0.0 0.0 94-95 2.6375 0.0 0.0 0.0 0.0 96-97 3.1875 0.0 0.0 0.0 0.0 98-99 3.525 0.0 0.0 0.0 0.0 100-101 3.9499999999999997 0.0 0.0 0.0 0.0 102-103 4.625 0.0 0.0 0.0 0.0 104-105 5.4875 0.0 0.0 0.0 0.0 106-107 6.275 0.0 0.0 0.0 0.0 108-109 7.050000000000001 0.0 0.0 0.0 0.0 110-111 7.5375 0.0 0.0 0.0 0.0 112-113 8.0625 0.0 0.0 0.0 0.0 114-115 8.3375 0.0 0.0 0.0 0.0 116-117 8.8 0.0 0.0 0.0 0.0 118-119 9.5875 0.0 0.0 0.0 0.0 120-121 10.274999999999999 0.0 0.0 0.0 0.0 122-123 10.9375 0.0 0.0 0.0 0.0 124-125 11.6625 0.0 0.0 0.0 0.0 126-127 12.4375 0.0 0.0 0.0 0.0 128-129 12.8875 0.0 0.0 0.0 0.0 130-131 13.7 0.0 0.0 0.0 0.0 132-133 14.8875 0.0 0.0 0.0 0.0 134-135 15.5875 0.0 0.0 0.0 0.0 136-137 16.3375 0.0 0.0 0.0 0.0 138-139 17.1125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAATT 10 0.006830828 145.0 9 ACGTAGA 10 0.006830828 145.0 5 TTCGTAT 40 0.0076550315 18.125 140-144 TCGTATC 40 0.0076550315 18.125 140-144 ACGGATT 40 0.0076550315 18.125 135-139 CAGTCAC 55 0.0025160722 15.818182 130-134 ACTCCAG 55 0.0025160722 15.818182 125-129 AACTCCA 55 0.0025160722 15.818182 125-129 AGTCACG 55 0.0025160722 15.818182 130-134 TCTGAAC 60 0.004491891 14.500001 120-124 AGAGCAC 95 0.007278115 10.684211 110-114 >>END_MODULE SRR13165365 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165365_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.052 37.0 37.0 37.0 37.0 37.0 2 36.109 37.0 37.0 37.0 37.0 37.0 3 36.049 37.0 37.0 37.0 37.0 37.0 4 36.147 37.0 37.0 37.0 37.0 37.0 5 36.2475 37.0 37.0 37.0 37.0 37.0 6 36.196 37.0 37.0 37.0 37.0 37.0 7 36.097 37.0 37.0 37.0 37.0 37.0 8 36.1325 37.0 37.0 37.0 37.0 37.0 9 35.95 37.0 37.0 37.0 37.0 37.0 10-14 36.0486 37.0 37.0 37.0 37.0 37.0 15-19 35.96220000000001 37.0 37.0 37.0 37.0 37.0 20-24 35.90915 37.0 37.0 37.0 37.0 37.0 25-29 35.72865 37.0 37.0 37.0 37.0 37.0 30-34 35.6057 37.0 37.0 37.0 37.0 37.0 35-39 35.55365 37.0 37.0 37.0 37.0 37.0 40-44 35.67165 37.0 37.0 37.0 37.0 37.0 45-49 35.7068 37.0 37.0 37.0 37.0 37.0 50-54 35.710249999999995 37.0 37.0 37.0 37.0 37.0 55-59 35.78855 37.0 37.0 37.0 37.0 37.0 60-64 35.83375 37.0 37.0 37.0 37.0 37.0 65-69 35.7354 37.0 37.0 37.0 37.0 37.0 70-74 35.57575 37.0 37.0 37.0 37.0 37.0 75-79 35.532050000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.5517 37.0 37.0 37.0 37.0 37.0 85-89 35.55050000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.59054999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.6802 37.0 37.0 37.0 37.0 37.0 100-104 35.636050000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.67925 37.0 37.0 37.0 37.0 37.0 110-114 35.53085 37.0 37.0 37.0 37.0 37.0 115-119 35.5362 37.0 37.0 37.0 37.0 37.0 120-124 35.362350000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.330600000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.271249999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.15255 37.0 37.0 37.0 32.2 37.0 140-144 34.99585 37.0 37.0 37.0 27.4 37.0 145-149 34.7472 37.0 37.0 37.0 25.0 37.0 150-151 34.4675 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 3.0 14 9.0 15 5.0 16 2.0 17 5.0 18 2.0 19 11.0 20 6.0 21 6.0 22 15.0 23 11.0 24 14.0 25 11.0 26 14.0 27 18.0 28 16.0 29 26.0 30 26.0 31 48.0 32 48.0 33 91.0 34 217.0 35 525.0 36 2600.0 37 267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.98994974874372 20.502512562814072 6.909547738693467 24.597989949748744 2 33.25 22.275 23.25 21.224999999999998 3 25.4 23.625 28.050000000000004 22.925 4 28.675 29.7 19.7 21.925 5 30.725 31.175000000000004 17.4 20.7 6 25.35 35.175 18.45 21.025 7 26.25 20.925 28.925 23.9 8 27.6 22.75 21.425 28.225 9 28.375 20.974999999999998 23.875 26.775 10-14 29.32 26.06 20.3 24.32 15-19 28.895 24.884999999999998 21.985 24.235 20-24 28.56571114112762 24.40342188203512 22.442343288808843 24.588523688028417 25-29 28.62646985238929 24.808606454841133 21.816362271703778 24.748561421065798 30-34 29.202521512907744 24.83490094056434 22.15829497698619 23.804282569541723 35-39 28.410625844214316 23.983190754915203 23.03767071889539 24.568512681975086 40-44 28.118276879971983 24.63101015660179 22.4145694701556 24.836143493270626 45-49 28.987392435461278 24.034420652391436 22.638583149889936 24.339603762257354 50-54 29.321126619640804 24.23332833058182 22.377307519135524 24.068237530641852 55-59 29.70227670753065 23.642732049036777 22.316737553164874 24.3382536902677 60-64 28.660763419880936 23.838110961028566 22.417329531242185 25.083796087848313 65-69 28.88366509952986 24.382314694408322 21.84655396618986 24.887466239871962 70-74 29.28696522391794 23.64773580185139 22.336752564423318 24.728546409807358 75-79 29.234002101365885 23.85550607895132 22.594686546255065 24.315805273427728 80-84 28.559279639819913 23.406703351675837 23.151575787893947 24.882441220610303 85-89 29.52066446512559 24.547183028119683 21.790253177224056 24.141899329530673 90-94 29.406173395367453 24.933713542448345 22.097153434388915 23.562959627795287 95-99 29.309654827413706 24.957478739369684 22.00600300150075 23.72686343171586 100-104 29.45709281961471 24.358268701526146 22.216662496872654 23.967975981986488 105-109 29.331998999249436 25.17888416312234 22.36177132849637 23.12734550913185 110-114 30.696883285807193 24.873680524288357 22.052128670768923 22.377307519135524 115-119 31.006704693285297 24.602221555088562 21.75522866006204 22.635845091564093 120-124 30.48786589942457 24.263197398048536 22.581936452339253 22.66700025018764 125-129 31.607124987491243 25.212648854197937 21.47002902031422 21.710197137996598 130-134 32.170562033932235 24.808568139732746 21.10004504279065 21.92082478354437 135-139 32.282440074062954 23.970374818595808 22.338988139918932 21.408196967422306 140-144 33.708539696833256 23.472910100555307 21.201660913502426 21.61688928910901 145-149 34.75280224179343 23.053442754203363 20.916733386709367 21.277021617293833 150-151 36.070087609511894 24.167709637046308 19.78723404255319 19.97496871088861 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 1.0 8 1.5 9 1.0 10 1.0 11 1.0 12 1.0 13 1.0 14 1.0 15 1.0 16 0.5 17 1.0 18 1.5 19 0.5 20 0.0 21 1.5 22 2.0 23 2.0 24 2.0 25 1.5 26 1.5 27 4.0 28 4.0 29 1.5 30 1.5 31 3.0 32 4.0 33 7.0 34 10.5 35 8.5 36 13.5 37 23.5 38 40.0 39 63.0 40 84.0 41 104.5 42 127.0 43 150.0 44 155.0 45 148.0 46 168.5 47 175.0 48 142.5 49 150.0 50 168.0 51 166.5 52 144.5 53 128.5 54 135.5 55 129.0 56 122.0 57 116.5 58 112.0 59 108.0 60 88.5 61 78.0 62 70.0 63 68.5 64 81.0 65 67.5 66 54.0 67 52.5 68 57.5 69 68.0 70 62.5 71 38.0 72 27.5 73 33.0 74 30.0 75 26.5 76 21.0 77 11.5 78 9.5 79 6.0 80 3.0 81 3.0 82 2.0 83 0.5 84 0.5 85 0.5 86 1.5 87 2.0 88 1.0 89 0.5 90 0.0 91 1.0 92 2.0 93 4.0 94 3.5 95 1.0 96 4.5 97 7.0 98 10.5 99 16.0 100 24.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.075 30-34 0.06 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.03 70-74 0.075 75-79 0.065 80-84 0.05 85-89 0.06999999999999999 90-94 0.055 95-99 0.05 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.06999999999999999 120-124 0.075 125-129 0.06999999999999999 130-134 0.095 135-139 0.08499999999999999 140-144 0.055 145-149 0.08 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.125 #Duplication Level Percentage of deduplicated Percentage of total 1 72.76672694394215 50.3 2 17.93851717902351 24.8 3 5.605786618444846 11.625 4 1.9891500904159132 5.5 5 0.9403254972875227 3.25 6 0.3978300180831827 1.6500000000000001 7 0.108499095840868 0.525 8 0.14466546112115733 0.8 9 0.0 0.0 >10 0.108499095840868 1.55 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 35 0.8750000000000001 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 16 0.4 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 11 0.27499999999999997 No Hit CGACCAACAACTACGCCCTGGACGAGATCAAGGAAGTTTAACATCAGAGA 8 0.2 No Hit GATCGAAGTGTACTAGCAGAGCAATACTTGTATTCAGCAAGCAAGCTGGC 8 0.2 No Hit AGTAGCGGCGAGCGAAATGGGAGCAGCCTAAACCGTGAAAACGGGGTTGT 8 0.2 No Hit CAGTACCGGACCCGCCTCTGCAAGGACGAGGTCGGCTGCGCCCGCCGCAT 8 0.2 No Hit CACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAG 7 0.17500000000000002 No Hit GCGTAAGCTATGACAATAAAAAGTGTGCTGTACCTGATGTGTCTGTGTGT 7 0.17500000000000002 No Hit CTACACTGCACGCTCTCCCCTCTTCCGATCTCGGTCTGCATCCTCCCGTC 7 0.17500000000000002 No Hit ACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAT 6 0.15 No Hit CCAACAGGCCGCTCCTCTCCTCCCCGCCCCGCCTCCCGAGAACCGCCGGC 6 0.15 No Hit GTTTCCTTGGCGCGCGACCGAGGAAAGGATGCTCCTGCTGCGCGCCAAGC 6 0.15 No Hit CTCATCATCTTGTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATTT 6 0.15 No Hit GGTGCTCCAGGCCATGGAGTCCCTGCAGGAGGTTGACGCGCTGGAGCGGT 6 0.15 No Hit CTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGT 6 0.15 No Hit CGTCACATACTGGTTATTGTTGATACAGGTAGTAGGATGTTTGATCTCGT 6 0.15 No Hit GTTGTAAGAGCCTAACCTGGAAGGAGTATGGTTCCGTGTCATATGCAACC 6 0.15 No Hit GGATGGCCCAAAGCCTATTTCAATTGTTGGTTCAACTGGTTCCATAGGAA 6 0.15 No Hit CAGAAACTGAAACCCTCTTCTCCCCACAGTTTCGCAGCAGCCTCTCCATC 6 0.15 No Hit GAGGGCCGCTGTTTGGGTTCACCAAGTCGAACGAGCTGTTCGTGGGGCGG 6 0.15 No Hit CACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTT 5 0.125 No Hit GATACTTGAACTGGAAGCTCTTAGCAGGACGCGGGCATGGAGAAGTGCAA 5 0.125 No Hit ATTGATGAAGTGCACACTTTGATTGGTGCTGGTGCAGCTGAGGGTGCAAT 5 0.125 No Hit GTCCGGATCGTGTGCGTCTGCCATGGGAACTTCCTGACCCCCGCCGAGTT 5 0.125 No Hit GTCTTGAACATTCTATACTTCGGTTTCTACATACTGTGCATTGTGGTCAC 5 0.125 No Hit GGAAATTGTAAGGGATATCAAGGAGAAGCTCGCATATGTGGCTCTTGACT 5 0.125 No Hit GTTTAGAACCAAGCGATTACAAAAAGATCGGAGCAAAGTTGAGGGATCTT 5 0.125 No Hit GCTTCAATCTCTGCAATTCATACGAAGATTACACGAGGATCCAGCTAAAT 5 0.125 No Hit ACTGCCCTTGCACCAAGCAGCATGAAGATTAAGGTGGTGGCACCGCCTGA 5 0.125 No Hit GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAA 5 0.125 No Hit CTAAGACCGTGGAAGAGGTTATGAGGATAGCAGATAAAGGGGCTGATTTT 5 0.125 No Hit CTTTGGGCCGGGTCGGCCGGTCCGCCTCACGGCGAGCACCGACCTACTCG 5 0.125 No Hit AGCAAGCACGCAAGGATATGAAAGTTCCTTGCGAAATCATCAGAGTCCCA 5 0.125 No Hit ACGACCCAATGTTCTACTACTGAGCTGCTCCTTGAGAGGACCTAACAAGG 5 0.125 No Hit AGCATCCAAGCCCGGTATCTGTCCTTGAACCTCTAGCTGAAGATTTCTCT 5 0.125 No Hit AACGGCTATACTTTGAACAGCGCCGGCCTCAAATGCAGTTCCAGTTAGCT 5 0.125 No Hit CCCAAATAGGTCAACCTTTTAAACTGCCTGCTGAATCCATGAGCAGGCAA 5 0.125 No Hit CCTGATGGTGGGGCTCGACGCCGCCGGGAAGACCACCATCCTCTACAAGC 5 0.125 No Hit GCTTATAGGACTCCGCCGGCACCTTATGAGAAATCAAAGTCTTTGGGTTC 5 0.125 No Hit GCCAACTGGTGCTACGCAACCGTCGCGCCCCGCGCTAAGAGCGTCGTCGT 5 0.125 No Hit GTTTGGAACATGGTTGAAAAGCATAAGAAGGGCGACTACACCTCTATTAT 5 0.125 No Hit ACTCAGTCATAGTTCACCTGCGTTGTACAAGCTTTCTTGTTACATATACA 5 0.125 No Hit AGCAAGCCCAAATACAGGCATGACTTCTACAATAGTACCACAGAAGTGGT 5 0.125 No Hit GTCCATGTCCCCGCTGCTGCTGCTGCCAAGGGCGCCGCCACTGCATAGAT 5 0.125 No Hit TGTCCCTGGCACCTACGGCTTCTACTGCGAGCCACATGCCGGGGCCGGCA 5 0.125 No Hit CAGGAGGAGAGGAGGAGGAGGATTAGGGCCAGGCAGCTCTTGAGGCAGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.0625 0.0 0.0 0.0 0.0 58-59 0.0875 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2875 0.0 0.0 0.0 0.0 76-77 0.4125 0.0 0.0 0.0 0.0 78-79 0.575 0.0 0.0 0.0 0.0 80-81 0.7125 0.0 0.0 0.0 0.0 82-83 1.075 0.0 0.0 0.0 0.0 84-85 1.425 0.0 0.0 0.0 0.0 86-87 1.6 0.0 0.0 0.0 0.0 88-89 1.7625000000000002 0.0 0.0 0.0 0.0 90-91 2.05 0.0 0.0 0.0 0.0 92-93 2.2750000000000004 0.0 0.0 0.0 0.0 94-95 2.6375 0.0 0.0 0.0 0.0 96-97 3.1875 0.0 0.0 0.0 0.0 98-99 3.6 0.0 0.0 0.0 0.0 100-101 4.025 0.0 0.0 0.0 0.0 102-103 4.7125 0.0 0.0 0.0 0.0 104-105 5.575 0.0 0.0 0.0 0.0 106-107 6.387499999999999 0.0 0.0 0.0 0.0 108-109 7.225 0.0 0.0 0.0 0.0 110-111 7.7625 0.0 0.0 0.0 0.0 112-113 8.2625 0.0 0.0 0.0 0.0 114-115 8.5625 0.0 0.0 0.0 0.0 116-117 9.0125 0.0 0.0 0.0 0.0 118-119 9.8375 0.0 0.0 0.0 0.0 120-121 10.5125 0.0 0.0 0.0 0.0 122-123 11.1875 0.0 0.0 0.0 0.0 124-125 11.9625 0.0 0.0 0.0 0.0 126-127 12.7625 0.0 0.0 0.0 0.0 128-129 13.2625 0.0 0.0 0.0 0.0 130-131 14.0625 0.0 0.0 0.0 0.0 132-133 15.2625 0.0 0.0 0.0 0.0 134-135 16.012500000000003 0.0 0.0 0.0 0.0 136-137 16.7125 0.0 0.0 0.0 0.0 138-139 17.475 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAGTT 10 0.006830828 145.0 3 >>END_MODULE Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313351 spots for SRR13165365.sra Written 1313351 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra Read 1313346 spots for SRR13165365.sra Written 1313346 spots for SRR13165365.sra SRR ids: ['SRR13165365.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_lr138d_r SRR13165365.sra spots: 26266925 blocks: [[1, 1313346], [1313347, 2626692], [2626693, 3940038], [3940039, 5253384], [5253385, 6566730], [6566731, 7880076], [7880077, 9193422], [9193423, 10506768], [10506769, 11820114], [11820115, 13133460], [13133461, 14446806], [14446807, 15760152], [15760153, 17073498], [17073499, 18386844], [18386845, 19700190], [19700191, 21013536], [21013537, 22326882], [22326883, 23640228], [23640229, 24953574], [24953575, 26266925]] SRR13165365 file size 8904949 SRR13165365 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165365 SRR13165365_1.fastq SRR13165365_2.fastq Input file: SRR13165365_1.fastq Paired file: SRR13165365_2.fastq trimmed: SRR13165365-trimmed-pair1.fastq, SRR13165365-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:23:50 2024 >> started Sat Dec 7 16:24:47 2024 >> done (57.212s) 26266925 read pairs processed; of these: 1076 ( 0.00%) short read pairs filtered out after trimming by size control 400059 ( 1.52%) empty read pairs filtered out after trimming by size control 25865790 (98.47%) read pairs available; of these: 6371571 (24.63%) trimmed read pairs available after processing 19494219 (75.37%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 88 0.00% 19 84 0.00% 20 147 0.00% 21 171 0.00% 22 176 0.00% 23 206 0.00% 24 266 0.00% 25 272 0.00% 26 320 0.00% 27 319 0.00% 28 413 0.00% 29 428 0.00% 30 518 0.00% 31 499 0.00% 32 586 0.00% 33 582 0.00% 34 584 0.00% 35 670 0.00% 36 626 0.00% 37 650 0.00% 38 727 0.00% 39 688 0.00% 40 859 0.00% 41 904 0.00% 42 888 0.00% 43 1029 0.00% 44 1113 0.00% 45 1191 0.00% 46 1183 0.00% 47 1268 0.00% 48 1495 0.01% 49 1476 0.01% 50 1774 0.01% 51 1985 0.01% 52 2082 0.01% 53 2187 0.01% 54 2409 0.01% 55 2482 0.01% 56 2822 0.01% 57 3052 0.01% 58 3203 0.01% 59 3542 0.01% 60 4120 0.02% 61 4273 0.02% 62 5175 0.02% 63 5387 0.02% 64 5775 0.02% 65 5960 0.02% 66 6590 0.03% 67 6931 0.03% 68 7496 0.03% 69 8391 0.03% 70 9118 0.04% 71 10160 0.04% 72 11641 0.05% 73 13217 0.05% 74 14486 0.06% 75 15064 0.06% 76 16102 0.06% 77 17711 0.07% 78 19140 0.07% 79 21029 0.08% 80 22703 0.09% 81 25171 0.10% 82 27291 0.11% 83 30254 0.12% 84 33586 0.13% 85 34788 0.13% 86 38587 0.15% 87 38955 0.15% 88 40893 0.16% 89 42997 0.17% 90 45616 0.18% 91 48649 0.19% 92 51399 0.20% 93 55133 0.21% 94 58480 0.23% 95 62260 0.24% 96 63257 0.24% 97 65518 0.25% 98 67074 0.26% 99 70025 0.27% 100 71311 0.28% 101 72891 0.28% 102 75196 0.29% 103 76350 0.30% 104 79295 0.31% 105 79935 0.31% 106 81741 0.32% 107 83857 0.32% 108 84972 0.33% 109 87479 0.34% 110 86770 0.34% 111 89150 0.34% 112 91736 0.35% 113 89991 0.35% 114 94121 0.36% 115 96928 0.37% 116 97610 0.38% 117 98831 0.38% 118 98714 0.38% 119 97571 0.38% 120 102104 0.39% 121 102190 0.40% 122 101880 0.39% 123 104431 0.40% 124 104439 0.40% 125 107155 0.41% 126 107598 0.42% 127 109374 0.42% 128 107788 0.42% 129 110512 0.43% 130 107349 0.42% 131 108649 0.42% 132 110799 0.43% 133 110475 0.43% 134 110734 0.43% 135 112683 0.44% 136 113476 0.44% 137 112406 0.43% 138 112173 0.43% 139 113398 0.44% 140 113954 0.44% 141 113506 0.44% 142 115018 0.44% 143 114305 0.44% 144 116777 0.45% 145 118893 0.46% 146 119944 0.46% 147 122216 0.47% 148 118197 0.46% 149 119768 0.46% 150 118565 0.46% 151 19494219 75.37% 25865790 reads passed initial QC criterion=sequence-density sequence-density=0.97 sequence-density-rank=1 fanout-score=2.25 fanout-score-rank=20 prefix-density=0.97 prefix-fanout=2.2 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.25 sequence-density-rank=16 fanout-score=20.94 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=20.9 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATTCGTATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=sequence-density sequence-density=0.63 sequence-density-rank=1 fanout-score=2.21 fanout-score-rank=28 prefix-density=0.66 prefix-fanout=2.1 sequence=GGTGGTGCATGGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=81.93 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=2.5 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGATGTCTGCTACCTTCTCGTCCACCGTCGGAGCTCCGGCTTCTACGCCAACCAGCTTCCTTGGGAAGAAGCTCAAGAAGCAGGTGACCTCGGCCGTGAACTACCATGGCAAGAGCACCAAGGCCAACAGATTCACAGTCATGGCCAAGGAGGTGGACGAGTCAAAGCAGACTGACCAGGACAGGTGGAAGGGCCTCGCCTACGATATCTCCGACGACCAGCAGGACATCACCAGGGGGAAGGGTATCGTCGACTCGCTCTTCCAGGCGCCCATGGGCGACGGTACCCACGTGGCCGTCCTCAGCTCCCAAGAGTACATCAGCCAGGGCCTAAGGAAGTACGACTTCGACAACA SRR13165365 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:48:57 Started mapping on | Dec 07 17:48:59 Finished on | Dec 07 17:53:51 Mapping speed, Million of reads per hour | 318.89 Number of input reads | 25865790 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 22106421 Uniquely mapped reads % | 85.47% Average mapped length | 286.04 Number of splices: Total | 19969408 Number of splices: Annotated (sjdb) | 18658972 Number of splices: GT/AG | 19691847 Number of splices: GC/AG | 236241 Number of splices: AT/AC | 9214 Number of splices: Non-canonical | 32106 Mismatch rate per base, % | 0.21% Deletion rate per base | 0.01% Deletion average length | 2.63 Insertion rate per base | 0.01% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1351320 % of reads mapped to multiple loci | 5.22% Number of reads mapped to too many loci | 318319 % of reads mapped to too many loci | 1.23% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.03% % of reads unmapped: other | 5.05% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2408311 2408311 2408311 N_multimapping 1351320 1351320 1351320 N_noFeature 1326233 21452649 1523744 N_ambiguous 549510 3242 94305 UnstrandedReadsAssigned:20230678 PositiveStrandReadsAssigned:650530 NegativeStrandReadsAssigned:20488372 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=141 echo kmer=137 SRR13165365 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165365-trimmed-pair1.fastq SRR13165365-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,865,790 reads, 21,092,158 reads pseudoaligned [quant] estimated average fragment length: 224.027 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,199 rounds 52973 SRR13165365.ke.tsv 35125 SRR13165365.se.tsv 88098 total ==> SRR13165365.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 713.27 0 0 PNS24247 1044 820.973 42.8023 3.23163 PNS24249 1928 1704.97 191.863 6.97523 PNS24246 1044 820.973 42.8023 3.23163 PNS24248 1044 820.973 42.8023 3.23163 PNS24244 1471 1247.97 175.73 8.72821 PNS24243 293 116.907 0 0 KQK14069 1603 1379.97 2047.29 91.9586 KQK14071 474 264.978 8.28229 1.93743 ==> SRR13165365.se.tsv <== BRADI_1g14170v3 2163 BRADI_1g53295v3 161 BRADI_1g59795v3 395 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 409 BRADI_1g74790v3 662 BRADI_1g09890v3 0 BRADI_1g77505v3 384 BRADI_1g48960v3 0 SRR13165365 completed mapping pipeline successfully