Starting /dee2/code/volunteer_pipeline.sh SRR13165366 current disk space = 1516105830400 free memory = 1607727400 SRR13165366 SRAfilesize dc5037d6bf0f5376f185582539d38835 SRR13165366.sra SRR13165366.sra file validated SRR13165366 is paired end SRR13165366 is conventional basespace SRR13165366 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165366_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.591 37.0 37.0 37.0 37.0 37.0 2 36.22375 37.0 37.0 37.0 37.0 37.0 3 36.4885 37.0 37.0 37.0 37.0 37.0 4 36.519 37.0 37.0 37.0 37.0 37.0 5 36.549 37.0 37.0 37.0 37.0 37.0 6 36.51 37.0 37.0 37.0 37.0 37.0 7 36.4495 37.0 37.0 37.0 37.0 37.0 8 36.587 37.0 37.0 37.0 37.0 37.0 9 36.558 37.0 37.0 37.0 37.0 37.0 10-14 36.5207 37.0 37.0 37.0 37.0 37.0 15-19 36.4865 37.0 37.0 37.0 37.0 37.0 20-24 36.5154 37.0 37.0 37.0 37.0 37.0 25-29 36.422 37.0 37.0 37.0 37.0 37.0 30-34 36.414699999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.3752 37.0 37.0 37.0 37.0 37.0 40-44 36.399 37.0 37.0 37.0 37.0 37.0 45-49 36.323800000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.3342 37.0 37.0 37.0 37.0 37.0 55-59 36.3368 37.0 37.0 37.0 37.0 37.0 60-64 36.3 37.0 37.0 37.0 37.0 37.0 65-69 36.2698 37.0 37.0 37.0 37.0 37.0 70-74 36.249 37.0 37.0 37.0 37.0 37.0 75-79 36.2411 37.0 37.0 37.0 37.0 37.0 80-84 36.1985 37.0 37.0 37.0 37.0 37.0 85-89 36.209900000000005 37.0 37.0 37.0 37.0 37.0 90-94 36.1815 37.0 37.0 37.0 37.0 37.0 95-99 36.0925 37.0 37.0 37.0 37.0 37.0 100-104 36.1191 37.0 37.0 37.0 37.0 37.0 105-109 36.038 37.0 37.0 37.0 37.0 37.0 110-114 36.0264 37.0 37.0 37.0 37.0 37.0 115-119 36.0296 37.0 37.0 37.0 37.0 37.0 120-124 35.895799999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.8063 37.0 37.0 37.0 37.0 37.0 130-134 35.7518 37.0 37.0 37.0 37.0 37.0 135-139 35.6114 37.0 37.0 37.0 37.0 37.0 140-144 35.450599999999994 37.0 37.0 37.0 37.0 37.0 145-149 35.1638 37.0 37.0 37.0 27.4 37.0 150-151 34.8575 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 2.0 21 1.0 22 1.0 23 0.0 24 3.0 25 4.0 26 5.0 27 12.0 28 11.0 29 32.0 30 29.0 31 51.0 32 47.0 33 86.0 34 155.0 35 342.0 36 2781.0 37 437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.199999999999996 10.05 5.7 37.05 2 23.39622641509434 10.08805031446541 32.57861635220126 33.937106918238996 3 20.849999999999998 13.750000000000002 25.6 39.800000000000004 4 26.724999999999998 20.0 21.75 31.525 5 29.099999999999998 27.575 23.150000000000002 20.175 6 24.349999999999998 31.4 22.35 21.9 7 20.225 23.775 35.975 20.025000000000002 8 20.875 23.825 29.725 25.575 9 20.075000000000003 21.15 32.7 26.075 10-14 23.45 25.25 25.75 25.55 15-19 24.240000000000002 23.96 25.34 26.46 20-24 24.215 24.654999999999998 24.69 26.44 25-29 24.525 24.645 24.79 26.040000000000003 30-34 24.09 24.69 24.715 26.505000000000003 35-39 24.04 23.785 25.080000000000002 27.095000000000002 40-44 24.245 24.79 24.895 26.07 45-49 24.645 23.415 25.39 26.55 50-54 24.2 24.595 25.124999999999996 26.08 55-59 24.81 24.18 25.255 25.755 60-64 24.52 24.16 24.834999999999997 26.484999999999996 65-69 24.87 24.115000000000002 25.085 25.929999999999996 70-74 24.995 24.055 24.435000000000002 26.515 75-79 24.41 24.455 24.349999999999998 26.784999999999997 80-84 24.245 24.295 24.98 26.479999999999997 85-89 25.415 24.005000000000003 24.38 26.200000000000003 90-94 24.335 24.735 24.12 26.810000000000002 95-99 24.285 24.48 24.13 27.105 100-104 24.355 25.480000000000004 24.610000000000003 25.555 105-109 23.985 24.57 24.36 27.084999999999997 110-114 24.52 24.925 23.335 27.22 115-119 24.68 25.629999999999995 23.145 26.545 120-124 24.5 25.485000000000003 23.505000000000003 26.51 125-129 23.935000000000002 25.509999999999998 23.54 27.015 130-134 24.04 24.93 23.835 27.195000000000004 135-139 23.89 24.605 24.295 27.21 140-144 24.169999999999998 25.729999999999997 23.53 26.57 145-149 23.95 24.355 24.0 27.694999999999997 150-151 25.2125 23.45 23.6125 27.725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.0 27 2.5 28 4.5 29 3.0 30 6.5 31 11.0 32 11.0 33 14.0 34 20.0 35 26.5 36 43.0 37 59.5 38 74.5 39 93.0 40 100.0 41 118.0 42 136.0 43 154.0 44 152.0 45 175.5 46 206.0 47 193.5 48 187.0 49 175.5 50 157.0 51 143.5 52 141.5 53 130.0 54 119.5 55 108.5 56 100.0 57 92.0 58 79.5 59 89.0 60 92.0 61 71.5 62 55.0 63 57.5 64 62.5 65 66.5 66 60.0 67 44.0 68 44.5 69 38.5 70 38.0 71 43.0 72 32.5 73 30.5 74 36.0 75 30.0 76 19.0 77 15.0 78 12.0 79 8.5 80 6.5 81 3.5 82 1.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.625 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.75 #Duplication Level Percentage of deduplicated Percentage of total 1 73.27526132404181 52.575 2 18.60627177700348 26.700000000000003 3 5.087108013937282 10.95 4 1.8815331010452963 5.4 5 0.8710801393728222 3.125 6 0.20905923344947736 0.8999999999999999 7 0.06968641114982578 0.35000000000000003 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCAAAGTCCCTCCCACCAAGAGACCGGTCATATGCATGTGATAACATCT 7 0.17500000000000002 No Hit GGTCACCGGCCCCCATGTATACTCGTTCCCGTTGTCCTTGAGCGTCTTTA 7 0.17500000000000002 No Hit GGGGCCCACTGTATGGATGACCTTCCTTGCAGGAAGATCATATGCATTGG 6 0.15 No Hit GGCCGATTCATGGAGCTGGTGTTGTAGGTGGCGAAGAACTCGAGCCGCAG 6 0.15 No Hit GTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGT 6 0.15 No Hit GCCATCAAGTCCTCGTACTGCCCAGTAGCAGCATTGTAGCCAAACTTGAC 6 0.15 No Hit GTTCGGAGAGGGAGACTAGACGCCCTGTGCGGATCTTGGTCAGGCCTTCC 6 0.15 No Hit CCCTGGAACAGACAATTGCTCCAACACCTCAAGCAAGGTACTTGTGCTCA 6 0.15 No Hit CTCCTCTTGACCATGCTCATCAAGCCATGGATATCCTCTACAGATCGAAA 5 0.125 No Hit ACTTCCTCAATACACACATCCAAAAAAATTGTATGGGCTACTGAGTCCCA 5 0.125 No Hit GGCATACTCAGTGAAATACTTAAATTTGACAGGACATTAGAAGACAACAT 5 0.125 No Hit GTGATTCCTCACCAGCAGAATTCAGACGAATAGCCTCATCTATTACATTC 5 0.125 No Hit GGGCATGCAGATCGTTCACCCACTTGAGGAGCCGGAAGACTTTGCGAGCC 5 0.125 No Hit TGTTGCAAAGCCTTGATCAGTGGCTTGCCCCGGTAACTGACATGGAATTT 5 0.125 No Hit GTTCGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAA 5 0.125 No Hit GGCTGGTCAAAGAAGAAATAATGTATTTGCAACAGGTCAGCTAACTCAGC 5 0.125 No Hit GCCGGACATGGCGTCGGGGCCGGCGCCGCGGCCGCCGCCGAAGAAGGGGA 5 0.125 No Hit GCCGGACTTGGTCAGGCTGGGCTCGCTGACCACCTGCGCCAGCCGCTTCC 5 0.125 No Hit GGGCTCTTATTCGCTGTACTCAGACGAATACGAGGTAGCGCCTTCATCTT 5 0.125 No Hit GCTGTCCATGAGCTCCTGCACCTTGGAAGGATCATTGGCACGAGTTCGCA 5 0.125 No Hit CCTTGCTATTTCCAATCTTGAACATTGCAGCAATCCCTTCGTACTGACTC 5 0.125 No Hit TGATGAGCTAGATTGAGATTGAGACTGCGGTGGCCATGGAGTGTTCCAAG 5 0.125 No Hit CTGGGGAAATTGAAAATTGATAGATGCTGCAAGCTGCAGTCCTTCAGCCC 5 0.125 No Hit CTCCATAGCACATCAAATTAGTTCAACGGAAAACCCCGGAGGCACGCGAC 5 0.125 No Hit ATCGCTTCCTCGACAGTCATATCAGGACGATACAACTTGTCCATCAGTGA 5 0.125 No Hit CTTTGATCTTTTAGCATACCGTAAAACTATAGCACATGTACAGTTTACTA 5 0.125 No Hit CCAGCATCCAGTCTCCATCCTTGTCTTCGTAAGTCAACACATATTCACCA 5 0.125 No Hit GTCTAGTTCAGCCTTGCTTTCGCTTTCAGTGGGTTCAATCATAAGTGTGC 5 0.125 No Hit ACTTGTTGTAGTTTGCCAGGCTCTCGATGCCACCACCCTGTGTAGACTTG 5 0.125 No Hit CGGCAAGCTCGACTACTTCTACTGGATCGTGACGCTGCTGCAGGTCTTGA 5 0.125 No Hit CCACAAGTAGCTTGGTTATAAGGTCAACAACTTCCACGCTGAATGTTGAC 5 0.125 No Hit GCCCTTCTTGCCCCTGGCGTACCGCTCATTCCTGTACTGCGGAGGGTTGC 5 0.125 No Hit GGAAGGTAATTTTTTGACGAACGTTTTGAAGGTAATTGAACTAGCGGCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1875 0.0 0.0 0.0 0.0 66-67 0.3125 0.0 0.0 0.0 0.0 68-69 0.4875 0.0 0.0 0.0 0.0 70-71 0.525 0.0 0.0 0.0 0.0 72-73 0.6375 0.0 0.0 0.0 0.0 74-75 0.775 0.0 0.0 0.0 0.0 76-77 1.1125 0.0 0.0 0.0 0.0 78-79 1.225 0.0 0.0 0.0 0.0 80-81 1.4125 0.0 0.0 0.0 0.0 82-83 1.8625 0.0 0.0 0.0 0.0 84-85 2.375 0.0 0.0 0.0 0.0 86-87 2.6500000000000004 0.0 0.0 0.0 0.0 88-89 3.1375 0.0 0.0 0.0 0.0 90-91 3.7625 0.0 0.0 0.0 0.0 92-93 4.2875 0.0 0.0 0.0 0.0 94-95 4.85 0.0 0.0 0.0 0.0 96-97 5.55 0.0 0.0 0.0 0.0 98-99 6.325 0.0 0.0 0.0 0.0 100-101 6.949999999999999 0.0 0.0 0.0 0.0 102-103 7.7 0.0 0.0 0.0 0.0 104-105 8.4375 0.0 0.0 0.0 0.0 106-107 9.575 0.0 0.0 0.0 0.0 108-109 10.3 0.0 0.0 0.0 0.0 110-111 11.05 0.0 0.0 0.0 0.0 112-113 11.787500000000001 0.0 0.0 0.0 0.0 114-115 12.825 0.0 0.0 0.0 0.0 116-117 13.7 0.0 0.0 0.0 0.0 118-119 14.7375 0.0 0.0 0.0 0.0 120-121 15.625 0.0 0.0 0.0 0.0 122-123 16.5375 0.0 0.0 0.0 0.0 124-125 17.112499999999997 0.0 0.0 0.0 0.0 126-127 18.075000000000003 0.0 0.0 0.0 0.0 128-129 18.975 0.0 0.0 0.0 0.0 130-131 20.0 0.0 0.0 0.0 0.0 132-133 20.925 0.0 0.0 0.0 0.0 134-135 22.0 0.0 0.0 0.0 0.0 136-137 22.875 0.0 0.0 0.0 0.0 138-139 23.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAGG 10 0.006830828 145.0 4 AACAGGA 10 0.006830828 145.0 5 GCTTCCT 10 0.006830828 145.0 9 >>END_MODULE SRR13165366 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165366_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.07925 37.0 37.0 37.0 37.0 37.0 2 36.258 37.0 37.0 37.0 37.0 37.0 3 36.3035 37.0 37.0 37.0 37.0 37.0 4 36.3535 37.0 37.0 37.0 37.0 37.0 5 36.411 37.0 37.0 37.0 37.0 37.0 6 36.3385 37.0 37.0 37.0 37.0 37.0 7 36.426 37.0 37.0 37.0 37.0 37.0 8 36.439 37.0 37.0 37.0 37.0 37.0 9 36.4495 37.0 37.0 37.0 37.0 37.0 10-14 36.356899999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.3196 37.0 37.0 37.0 37.0 37.0 20-24 36.38015 37.0 37.0 37.0 37.0 37.0 25-29 36.30675 37.0 37.0 37.0 37.0 37.0 30-34 36.29515 37.0 37.0 37.0 37.0 37.0 35-39 36.27455 37.0 37.0 37.0 37.0 37.0 40-44 36.268649999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.2492 37.0 37.0 37.0 37.0 37.0 50-54 36.16295 37.0 37.0 37.0 37.0 37.0 55-59 36.22175 37.0 37.0 37.0 37.0 37.0 60-64 36.14735 37.0 37.0 37.0 37.0 37.0 65-69 36.0945 37.0 37.0 37.0 37.0 37.0 70-74 36.10625 37.0 37.0 37.0 37.0 37.0 75-79 36.0603 37.0 37.0 37.0 37.0 37.0 80-84 36.0788 37.0 37.0 37.0 37.0 37.0 85-89 36.025549999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.9542 37.0 37.0 37.0 37.0 37.0 95-99 35.99565 37.0 37.0 37.0 37.0 37.0 100-104 35.91225 37.0 37.0 37.0 37.0 37.0 105-109 35.81885 37.0 37.0 37.0 37.0 37.0 110-114 35.72535 37.0 37.0 37.0 37.0 37.0 115-119 35.676249999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.51925 37.0 37.0 37.0 37.0 37.0 125-129 35.436400000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.06335 37.0 37.0 37.0 27.4 37.0 135-139 34.9079 37.0 37.0 37.0 25.0 37.0 140-144 34.68195000000001 37.0 37.0 37.0 25.0 37.0 145-149 34.4088 37.0 37.0 37.0 25.0 37.0 150-151 34.19375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 1.0 15 6.0 16 1.0 17 0.0 18 1.0 19 0.0 20 0.0 21 2.0 22 2.0 23 1.0 24 6.0 25 4.0 26 9.0 27 5.0 28 23.0 29 16.0 30 33.0 31 38.0 32 71.0 33 140.0 34 231.0 35 513.0 36 2604.0 37 290.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.1792618629174 18.227466733617874 9.942254581973387 29.65101682149134 2 30.025000000000002 23.175 24.0 22.8 3 23.35 24.025 29.75 22.875 4 27.05 28.275 21.4 23.275000000000002 5 27.55 32.05 19.400000000000002 21.0 6 22.6 36.275 17.849999999999998 23.275000000000002 7 23.525 18.875 33.575 24.025 8 23.25 22.175 23.35 31.225 9 24.825 21.4 26.075 27.700000000000003 10-14 25.89 26.174999999999997 22.425 25.509999999999998 15-19 26.46 24.265 24.145 25.130000000000003 20-24 26.441322066103307 25.171258562928145 23.811190559527976 24.576228811440572 25-29 26.18154538634659 24.66116529132283 23.925981495373843 25.23130782695674 30-34 25.92888933340001 24.748712306846027 24.378656798519778 24.943741561234184 35-39 25.501275063753187 25.226261313065653 23.941197059852993 25.331266563328164 40-44 25.09876481472221 25.023753563034457 23.723558533780068 26.153923088463273 45-49 26.257625762576257 24.202420242024203 24.122412241224122 25.417541754175417 50-54 25.656282814140706 24.70123506175309 24.331216560828043 25.311265563278162 55-59 26.971742935733932 24.346086521630408 23.885971492873217 24.79619904976244 60-64 26.761338066903345 24.53122656132807 23.491174558727938 25.216260813040652 65-69 26.545 25.540000000000003 23.45 24.465 70-74 27.121780445111277 24.07601900475119 24.541135283820957 24.261065266316578 75-79 27.520504100820165 24.41488297659532 23.2746549309862 24.789957991598317 80-84 26.82 25.540000000000003 23.32 24.32 85-89 27.836959239809957 24.45111277819455 23.025756439109777 24.68617154288572 90-94 26.687668766876687 25.04250425042504 23.98739873987399 24.282428242824285 95-99 28.376418820941048 25.49127456372819 22.541127056352817 23.59117955897795 100-104 28.13703425856464 25.926481620405102 22.50562640660165 23.43085771442861 105-109 28.637159289822456 25.206301575393848 23.470867716929234 22.685671417854465 110-114 29.066453322666135 26.071303565178262 22.456122806140307 22.4061203060153 115-119 30.307576894223555 24.831207801950487 22.420605151287823 22.440610152538135 120-124 29.127281820455114 25.35633908477119 22.535633908477116 22.980745186296573 125-129 30.971194238847772 25.18003600720144 22.744548909781955 21.104220844168832 130-134 31.045866053118594 25.083779322762968 22.222777972290302 21.64757665182814 135-139 31.884565369610883 24.59737921376413 22.681804541362407 20.83625087526258 140-144 32.921646082304115 24.23121156057803 21.826091304565228 21.02105105255263 145-149 33.38501550465139 24.227268180454136 21.491447434230267 20.8962688806642 150-151 35.71785892946473 23.449224612306153 20.635317658829415 20.1975987993997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 1.0 27 2.5 28 6.0 29 6.0 30 5.0 31 5.0 32 7.5 33 13.0 34 18.5 35 26.5 36 37.0 37 46.0 38 73.0 39 94.5 40 102.5 41 113.5 42 120.5 43 144.5 44 181.5 45 205.0 46 183.0 47 163.5 48 166.5 49 177.0 50 151.5 51 134.0 52 137.5 53 114.0 54 124.5 55 115.5 56 107.5 57 108.0 58 87.5 59 82.0 60 85.0 61 82.0 62 64.0 63 57.0 64 59.5 65 70.5 66 80.5 67 59.5 68 44.5 69 48.5 70 44.5 71 42.0 72 42.0 73 41.0 74 31.5 75 21.0 76 18.5 77 13.5 78 9.5 79 6.5 80 3.0 81 1.0 82 1.5 83 1.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.02 80-84 0.0 85-89 0.025 90-94 0.01 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.034999999999999996 135-139 0.03 140-144 0.005 145-149 0.03 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.75 #Duplication Level Percentage of deduplicated Percentage of total 1 74.00696864111498 53.1 2 17.526132404181187 25.15 3 5.331010452961673 11.475 4 1.9163763066202089 5.5 5 0.8710801393728222 3.125 6 0.20905923344947736 0.8999999999999999 7 0.06968641114982578 0.35000000000000003 8 0.06968641114982578 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 8 0.2 No Hit CACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAG 8 0.2 No Hit CTCGACCCGGACTTCGACAAGAAGGCCTTCCGCCACAACCTCACACGCAG 7 0.17500000000000002 No Hit CAGCTGAATGTCGCATTTGTTGATGTCGGGCATGCCAGCATGCAGGTCAG 7 0.17500000000000002 No Hit CGGAAGCAGATATCAAGCCCAAAATCAGGAGGTACGAGCGCCGCGCCATG 6 0.15 No Hit GTCCTGGGCTGCACGCTGCAGCAGGTTCAGGGCTCGCGGAGGAATGCACC 6 0.15 No Hit GCGTGGACAGTCATCGGCTGGCCACCAACAGGTTCGCGGACCTCACGGAC 6 0.15 No Hit GTTTACACTCGCATTGAACTTGAGCAGGTGACCGTGGAGCATGCTTTGGA 6 0.15 No Hit TCGGGATCGGAGTAATGATTAATAGGGACAGTCGGGGGCATTCGTATTTC 6 0.15 No Hit CTTAAAGAGAAAAAGTTGAGAGTTGAGGATGCTCTAAATGCAACTAAGGC 6 0.15 No Hit CATGCCGCTCAGCACCGCCGCCACCGCCAATTTCACCCGCGGCGAGCTGG 5 0.125 No Hit AGCTGCAGGGGCTTGAACGTGTAGCGCCTGGCGCATATGACGTAGTAGAC 5 0.125 No Hit CCTAGTGGTCGTGTTAAGCTTGCAGACTTTGGAATGGCTAAACATATCAA 5 0.125 No Hit GTCTCTCCGTTTCTCCTCTGACGCTGCTGGTTCTGTCTCTCCGTTTCTCC 5 0.125 No Hit GACAGAAAGGCCGTGTGGAAATGGCTGCAGCGGCCGCGGGAGCACCGATG 5 0.125 No Hit GGGTGACCTTCGCGTCGCTCTACCCGGGCTGCATCGCCACCACGGGGCTC 5 0.125 No Hit ATGTGGCCATTCAGCTGCGCACCTTCACCTGCCTATTTCACCTCAAGTGT 5 0.125 No Hit GGTGAACTTAGCTGATGTTGGGATCGTCGGCGGCCTCAGTGATGGATCTG 5 0.125 No Hit GATCCACAGAATTCCGTAGGTTGTGTGAGATTATCAGAAACCATCTTGCA 5 0.125 No Hit CTTCTGCCCGCCAAGGGCGCCAAGCGCGTGTTCGCTGACGAGGTCGCGCC 5 0.125 No Hit GTCGGAATACCTGACTAATAAGTACGGTTGGATTGATTGGCTTGCATGAC 5 0.125 No Hit AGGACATACACAGTATGTGGCATTGCTGACTCGCTAGCTCCAGAAATCGT 5 0.125 No Hit CTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGA 5 0.125 No Hit CAGGGTCTGGAGAGAACACCCCGAGCGGATGGTCGGCTTTTACCCACGGA 5 0.125 No Hit GTACGACTGACAATTATTAACAACATGCTTGAGTATCATCCTGAAACTAG 5 0.125 No Hit TGAGATTTCGTCGGAGCCACCGTACACAGAATATGTGTCAACTCGCTGGT 5 0.125 No Hit CAGGAGAGCTCTTAAGCTCACTCCTGTTTGCTTGTCTTATATTTTGGCCA 5 0.125 No Hit CGGAGGGTGATGAGGACGGGGAAGACGATGACGTCAACCATGCTGCTGCT 5 0.125 No Hit GCTCTATCCATCCCCTCCCTTCGCTTGCGGCTCCAGTTCCAGCTAGCGAG 5 0.125 No Hit GAGTTGAACCGCAACCGATTGATTGCTTTGATGTCTGCCATAGTTCTTGA 5 0.125 No Hit CCTCACGGCGAGCACCGACCTACTCGACCCTTCAGCCGGCGATGCGCTCC 5 0.125 No Hit GTGGAATTGTGAATTCGTCTGTTTGTTCGGTAGATTGCGTGTGTAATGTT 5 0.125 No Hit CAATGGCAGCACCTATTTCTAATTATTGCCCTTTCTTTTCTCATGAATAT 5 0.125 No Hit AAAGCATGACAGCAACCAATGGGCATTAATCAAGAGTGAAATAAGCAAGG 5 0.125 No Hit AAAAACTCGATCCGTTCCCCTTCTCGCCCCGCGGCTTGCGAACCCTAGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1875 0.0 0.0 0.0 0.0 66-67 0.3125 0.0 0.0 0.0 0.0 68-69 0.4875 0.0 0.0 0.0 0.0 70-71 0.525 0.0 0.0 0.0 0.0 72-73 0.6375 0.0 0.0 0.0 0.0 74-75 0.775 0.0 0.0 0.0 0.0 76-77 1.1125 0.0 0.0 0.0 0.0 78-79 1.275 0.0 0.0 0.0 0.0 80-81 1.4625 0.0 0.0 0.0 0.0 82-83 1.9124999999999999 0.0 0.0 0.0 0.0 84-85 2.425 0.0 0.0 0.0 0.0 86-87 2.7 0.0 0.0 0.0 0.0 88-89 3.1875 0.0 0.0 0.0 0.0 90-91 3.8125 0.0 0.0 0.0 0.0 92-93 4.3375 0.0 0.0 0.0 0.0 94-95 4.875 0.0 0.0 0.0 0.0 96-97 5.575 0.0 0.0 0.0 0.0 98-99 6.324999999999999 0.0 0.0 0.0 0.0 100-101 6.949999999999999 0.0 0.0 0.0 0.0 102-103 7.7 0.0 0.0 0.0 0.0 104-105 8.425 0.0 0.0 0.0 0.0 106-107 9.5625 0.0 0.0 0.0 0.0 108-109 10.3 0.0 0.0 0.0 0.0 110-111 11.024999999999999 0.0 0.0 0.0 0.0 112-113 11.8 0.0 0.0 0.0 0.0 114-115 12.825 0.0 0.0 0.0 0.0 116-117 13.7 0.0 0.0 0.0 0.0 118-119 14.775 0.0 0.0 0.0 0.0 120-121 15.725000000000001 0.0 0.0 0.0 0.0 122-123 16.6875 0.0 0.0 0.0 0.0 124-125 17.262500000000003 0.0 0.0 0.0 0.0 126-127 18.25 0.0 0.0 0.0 0.0 128-129 19.15 0.0 0.0 0.0 0.0 130-131 20.174999999999997 0.0 0.0 0.0 0.0 132-133 21.1 0.0 0.0 0.0 0.0 134-135 22.2125 0.0 0.0 0.0 0.0 136-137 23.075 0.0 0.0 0.0 0.0 138-139 23.862499999999997 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGGGG 515 0.0 12.669903 145 >>END_MODULE Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562737 spots for SRR13165366.sra Written 1562737 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra Read 1562726 spots for SRR13165366.sra Written 1562726 spots for SRR13165366.sra SRR ids: ['SRR13165366.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ikqfnbuf SRR13165366.sra spots: 31254531 blocks: [[1, 1562726], [1562727, 3125452], [3125453, 4688178], [4688179, 6250904], [6250905, 7813630], [7813631, 9376356], [9376357, 10939082], [10939083, 12501808], [12501809, 14064534], [14064535, 15627260], [15627261, 17189986], [17189987, 18752712], [18752713, 20315438], [20315439, 21878164], [21878165, 23440890], [23440891, 25003616], [25003617, 26566342], [26566343, 28129068], [28129069, 29691794], [29691795, 31254531]] SRR13165366 file size 10599956 SRR13165366 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165366 SRR13165366_1.fastq SRR13165366_2.fastq Input file: SRR13165366_1.fastq Paired file: SRR13165366_2.fastq trimmed: SRR13165366-trimmed-pair1.fastq, SRR13165366-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Thu Dec 12 02:14:59 2024 >> started Thu Dec 12 02:15:36 2024 >> done (37.203s) 31254531 read pairs processed; of these: 639 ( 0.00%) short read pairs filtered out after trimming by size control 25068 ( 0.08%) empty read pairs filtered out after trimming by size control 31228824 (99.92%) read pairs available; of these: 9394320 (30.08%) trimmed read pairs available after processing 21834504 (69.92%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 23 0.00% 19 17 0.00% 20 30 0.00% 21 49 0.00% 22 47 0.00% 23 47 0.00% 24 50 0.00% 25 63 0.00% 26 76 0.00% 27 75 0.00% 28 108 0.00% 29 102 0.00% 30 136 0.00% 31 157 0.00% 32 172 0.00% 33 213 0.00% 34 188 0.00% 35 205 0.00% 36 264 0.00% 37 274 0.00% 38 361 0.00% 39 370 0.00% 40 467 0.00% 41 519 0.00% 42 575 0.00% 43 645 0.00% 44 719 0.00% 45 740 0.00% 46 824 0.00% 47 997 0.00% 48 1209 0.00% 49 1501 0.00% 50 1744 0.01% 51 1978 0.01% 52 2198 0.01% 53 2385 0.01% 54 2607 0.01% 55 2814 0.01% 56 3177 0.01% 57 3631 0.01% 58 4256 0.01% 59 4906 0.02% 60 5627 0.02% 61 6539 0.02% 62 7487 0.02% 63 8497 0.03% 64 9300 0.03% 65 10159 0.03% 66 11135 0.04% 67 12209 0.04% 68 13907 0.04% 69 15256 0.05% 70 17296 0.06% 71 19259 0.06% 72 21881 0.07% 73 24858 0.08% 74 27183 0.09% 75 29869 0.10% 76 32397 0.10% 77 34840 0.11% 78 37988 0.12% 79 41038 0.13% 80 44032 0.14% 81 48304 0.15% 82 53207 0.17% 83 58661 0.19% 84 65903 0.21% 85 68788 0.22% 86 74380 0.24% 87 75849 0.24% 88 79998 0.26% 89 82455 0.26% 90 85673 0.27% 91 90463 0.29% 92 94277 0.30% 93 99111 0.32% 94 104886 0.34% 95 109254 0.35% 96 111730 0.36% 97 116111 0.37% 98 117925 0.38% 99 121019 0.39% 100 124405 0.40% 101 123872 0.40% 102 127472 0.41% 103 129587 0.41% 104 132214 0.42% 105 133652 0.43% 106 136771 0.44% 107 135768 0.43% 108 138227 0.44% 109 139740 0.45% 110 138370 0.44% 111 139623 0.45% 112 141637 0.45% 113 141103 0.45% 114 144036 0.46% 115 146473 0.47% 116 146888 0.47% 117 145896 0.47% 118 147751 0.47% 119 145787 0.47% 120 149001 0.48% 121 145849 0.47% 122 145580 0.47% 123 147792 0.47% 124 148140 0.47% 125 149975 0.48% 126 148838 0.48% 127 150557 0.48% 128 148095 0.47% 129 147870 0.47% 130 147602 0.47% 131 147655 0.47% 132 145387 0.47% 133 147474 0.47% 134 145462 0.47% 135 145087 0.46% 136 145260 0.47% 137 143438 0.46% 138 142505 0.46% 139 142924 0.46% 140 143469 0.46% 141 142535 0.46% 142 143327 0.46% 143 141459 0.45% 144 141287 0.45% 145 143030 0.46% 146 141281 0.45% 147 146145 0.47% 148 142666 0.46% 149 144761 0.46% 150 140927 0.45% 151 21834504 69.92% 31228824 reads passed initial QC criterion=sequence-density sequence-density=0.99 sequence-density-rank=1 fanout-score=2.38 fanout-score-rank=29 prefix-density=0.99 prefix-fanout=2.4 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=73.25 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=6.3 sequence=AATATCTTTATATATGAACAAAAACTTTTCATGCCCAGCAATTGCTTGGATGCAATGCGGTACTTAGGTACAAAGAGTGAAACATCAGAATAATTAAAGTGGCATGCTTAAAAGGTGTAAAGGCAGCTGCCGTCGTCACTCCTTGCTGTTGGGTCGTAGTTCTCGGCATTCCGGTCAGTGCAACCTTCTGGGACGGGCAAATTACCTTGTTGTGCTCCTTTACCTCCTCCTATGCAGCTAGAGATGGTGTGTGTATGAAGAGTGTTCTAACCGTAGAAGGAACCAGTCTTCATGGCATCTGAGTTAGCATCTCCCAGAGCAGCCTCGCTCATGTACTTGTCAGCAAGCTGCACACGCTTGACATTGTCCTGCTCTTGGACGAGCATGTGGCCGTACTCCAGGAGCTTCTCGATTGTCATCTTTGGCTGCTCAAAGGACACCGGTCCATCCTTCGAGTTCACCAGCTTCTTGCCGATGTTCTCTATTCCGGTTGAGCTGACCCACTTGCGCA criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.99 fanout-score-rank=27 prefix-density=0.50 prefix-fanout=2.5 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.11 sequence-density-rank=14 fanout-score=124.38 fanout-score-rank=1 prefix-density=0.75 prefix-fanout=18.0 sequence=GCCGCCGCCGCCA SRR13165366 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 12 02:16:24 Started mapping on | Dec 12 02:16:24 Finished on | Dec 12 02:18:47 Mapping speed, Million of reads per hour | 786.18 Number of input reads | 31228824 Average input read length | 281 UNIQUE READS: Uniquely mapped reads number | 29245981 Uniquely mapped reads % | 93.65% Average mapped length | 280.37 Number of splices: Total | 28693309 Number of splices: Annotated (sjdb) | 26856957 Number of splices: GT/AG | 28290656 Number of splices: GC/AG | 341590 Number of splices: AT/AC | 14037 Number of splices: Non-canonical | 47026 Mismatch rate per base, % | 0.21% Deletion rate per base | 0.01% Deletion average length | 2.58 Insertion rate per base | 0.01% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 606156 % of reads mapped to multiple loci | 1.94% Number of reads mapped to too many loci | 159588 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.12% % of reads unmapped: other | 1.77% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1377042 1377042 1377042 N_multimapping 606156 606156 606156 N_noFeature 1212406 28421281 1490492 N_ambiguous 649240 4473 103149 UnstrandedReadsAssigned:27384335 PositiveStrandReadsAssigned:820227 NegativeStrandReadsAssigned:27652340 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=129 echo kmer=125 SRR13165366 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165366-trimmed-pair1.fastq SRR13165366-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,228,824 reads, 27,975,594 reads pseudoaligned [quant] estimated average fragment length: 217.194 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,235 rounds 52973 SRR13165366.ke.tsv 35125 SRR13165366.se.tsv 88098 total ==> SRR13165366.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 720.285 0 0 PNS24247 1044 827.806 69.7044 4.32168 PNS24249 1928 1711.81 236.505 7.09099 PNS24246 1044 827.806 69.7044 4.32168 PNS24248 1044 827.806 69.7044 4.32168 PNS24244 1471 1254.81 114.382 4.67845 PNS24243 293 125.877 0 0 KQK14069 1603 1386.81 670.307 24.8073 KQK14071 474 273.732 36.0387 6.75718 ==> SRR13165366.se.tsv <== BRADI_1g14170v3 747 BRADI_1g53295v3 197 BRADI_1g59795v3 614 BRADI_1g07683v3 0 BRADI_1g00485v3 8 BRADI_1g20270v3 447 BRADI_1g74790v3 943 BRADI_1g09890v3 1 BRADI_1g77505v3 412 BRADI_1g48960v3 0 SRR13165366 completed mapping pipeline successfully