Starting /dee2/code/volunteer_pipeline.sh SRR13165367 current disk space = 1541912166400 free memory = 1533670480 SRR13165367 SRAfilesize 1956b6036b3b0a36a80efc3d32b05c1a SRR13165367.sra SRR13165367.sra file validated SRR13165367 is paired end SRR13165367 is conventional basespace SRR13165367 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165367_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5225 37.0 37.0 37.0 37.0 37.0 2 36.0975 37.0 37.0 37.0 37.0 37.0 3 36.4765 37.0 37.0 37.0 37.0 37.0 4 36.526 37.0 37.0 37.0 37.0 37.0 5 36.4295 37.0 37.0 37.0 37.0 37.0 6 36.5625 37.0 37.0 37.0 37.0 37.0 7 36.504 37.0 37.0 37.0 37.0 37.0 8 36.4655 37.0 37.0 37.0 37.0 37.0 9 36.495 37.0 37.0 37.0 37.0 37.0 10-14 36.5078 37.0 37.0 37.0 37.0 37.0 15-19 36.478300000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.4234 37.0 37.0 37.0 37.0 37.0 25-29 36.427200000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.351099999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.3408 37.0 37.0 37.0 37.0 37.0 40-44 36.328199999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.307500000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.294399999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3093 37.0 37.0 37.0 37.0 37.0 60-64 36.258799999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.1914 37.0 37.0 37.0 37.0 37.0 70-74 36.241499999999995 37.0 37.0 37.0 37.0 37.0 75-79 36.2097 37.0 37.0 37.0 37.0 37.0 80-84 36.09590000000001 37.0 37.0 37.0 37.0 37.0 85-89 36.187400000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.1161 37.0 37.0 37.0 37.0 37.0 95-99 36.0381 37.0 37.0 37.0 37.0 37.0 100-104 36.069 37.0 37.0 37.0 37.0 37.0 105-109 36.0749 37.0 37.0 37.0 37.0 37.0 110-114 36.0714 37.0 37.0 37.0 37.0 37.0 115-119 35.9854 37.0 37.0 37.0 37.0 37.0 120-124 35.937 37.0 37.0 37.0 37.0 37.0 125-129 35.8709 37.0 37.0 37.0 37.0 37.0 130-134 35.935300000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.834700000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.7361 37.0 37.0 37.0 37.0 37.0 145-149 35.5944 37.0 37.0 37.0 37.0 37.0 150-151 35.51475 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 1.0 25 10.0 26 6.0 27 7.0 28 19.0 29 31.0 30 39.0 31 49.0 32 58.0 33 74.0 34 124.0 35 299.0 36 2836.0 37 445.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.650000000000006 9.525 3.775 27.05 2 26.136363636363637 9.04040404040404 32.55050505050505 32.27272727272727 3 22.425 15.8 25.25 36.525 4 28.4 17.925 23.625 30.049999999999997 5 27.400000000000002 26.125 22.625 23.849999999999998 6 26.5 29.275000000000002 20.5 23.724999999999998 7 20.625 23.849999999999998 38.0 17.525 8 22.125 25.074999999999996 28.499999999999996 24.3 9 21.775 21.075 32.5 24.65 10-14 25.480000000000004 24.66 25.15 24.709999999999997 15-19 25.345000000000002 24.154999999999998 24.645 25.855 20-24 24.915000000000003 24.285 24.535 26.265 25-29 24.36 25.259999999999998 24.240000000000002 26.14 30-34 25.06 23.96 23.830000000000002 27.150000000000002 35-39 24.235 24.45 25.445 25.869999999999997 40-44 25.27 23.855 23.595 27.279999999999998 45-49 24.169999999999998 24.709999999999997 23.885 27.235 50-54 25.019999999999996 24.060000000000002 24.215 26.705000000000002 55-59 25.174999999999997 24.52 24.275 26.029999999999998 60-64 25.285000000000004 23.98 24.63 26.105 65-69 25.495 24.5 23.65 26.355 70-74 25.185000000000002 24.855 23.669999999999998 26.290000000000003 75-79 24.88 24.39 24.22 26.51 80-84 24.89 24.23 24.29 26.590000000000003 85-89 24.725 24.575 23.965 26.735 90-94 25.395 23.71 23.845 27.05 95-99 25.56 24.22 23.855 26.365 100-104 25.1 25.019999999999996 23.330000000000002 26.55 105-109 25.874999999999996 23.87 23.825 26.43 110-114 25.105 24.77 23.09 27.034999999999997 115-119 24.375 24.245 24.255 27.125 120-124 26.08 23.794999999999998 23.445 26.68 125-129 24.845 24.5 23.515 27.139999999999997 130-134 25.455 24.89 23.080000000000002 26.575 135-139 25.019999999999996 24.145 23.275000000000002 27.560000000000002 140-144 25.94 24.445 24.085 25.53 145-149 25.03 24.16 23.77 27.04 150-151 25.1 24.349999999999998 23.0375 27.5125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 3.5 26 5.0 27 3.0 28 3.5 29 3.0 30 4.5 31 8.0 32 8.5 33 13.0 34 24.0 35 34.0 36 42.0 37 47.0 38 67.5 39 87.5 40 101.0 41 110.5 42 133.0 43 167.5 44 160.0 45 164.5 46 177.0 47 173.5 48 152.5 49 136.0 50 137.5 51 129.0 52 127.5 53 111.5 54 105.0 55 95.0 56 97.0 57 114.5 58 121.0 59 116.0 60 107.0 61 89.0 62 71.0 63 71.0 64 70.0 65 80.5 66 82.0 67 67.5 68 54.5 69 52.5 70 49.5 71 43.0 72 43.0 73 43.5 74 25.5 75 16.0 76 14.0 77 9.0 78 8.5 79 6.0 80 4.0 81 3.0 82 3.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.89999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 72.74330042313117 51.575 2 17.983074753173483 25.5 3 6.558533145275034 13.950000000000001 4 1.6572637517630464 4.7 5 0.5994358251057829 2.125 6 0.2820874471086037 1.2 7 0.10578279266572638 0.525 8 0.03526093088857546 0.2 9 0.03526093088857546 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTTCATATCCACGGTAGCTCTTCCTTTGAGAAGAGGCATGCTGCTGTAT 9 0.22499999999999998 No Hit GCATGTCCTCATCCACCCCAAGCGCCCGGAGCACATAGGAAGGCTCCAAG 8 0.2 No Hit GTGTGATGATGCAGTAGTCAGACCCTTCTTCGACATGTGCTCCCAGCTTT 7 0.17500000000000002 No Hit GGCCCAGAAGCATGGCGCATTACACCAGAGAAGTCATTCATCCATGATGG 7 0.17500000000000002 No Hit GTTGCCATCTGAGTGATGATCCAAAAATTCTAGAACAATAACTGAAAAGC 7 0.17500000000000002 No Hit CCCTAGTAAGGTCAATACAAGTAGATATATGCCTTTTTCTCCCTCCCTCT 6 0.15 No Hit ATCGCTTCGAGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGC 6 0.15 No Hit GCACTGTGAAGTTGAGGATCAGTGGCACTGGGATGTAGCCGAGGATGGCA 6 0.15 No Hit GTGTGGGAGCTCGGACCTTCCCACTCTTAATTGCAGTTTTAAGATCCTCA 6 0.15 No Hit GCTTGAGTTCAGCAGGTTGTCCATGAAAGTCAAATTTCACAACCTTATCC 6 0.15 No Hit GGTGGGATACAGGGATTCGACAGATACATTATCTTTTGATGGGATACGGG 6 0.15 No Hit CCTCGAACTGCTCCTGTTTCTTCTCCGGCGCCATGCCGAGAACCACCACC 6 0.15 No Hit CTCGGAAGCAGGGATTCCACTTTTCTTTGACATCCAGTCTACTACTTCTC 6 0.15 No Hit GTCCGTCTATACAATCACAACTTTGAGTGTTTGAACTCGAAAAGCTATAC 5 0.125 No Hit GTCGCAGACAAACAAGACGCACCTTTGTATGGCATGATACCAAGCCAGTC 5 0.125 No Hit GGGTGCGGAGGGTGAGGAGGAAGAGGAAGGACGCGGCGGAGGCGGCGGCG 5 0.125 No Hit AGGAAAACATCATCCAAATCGGCAACCACCATCATTTGAGGTTGACTCAA 5 0.125 No Hit ATCCAGCTTTTGTTTTCAGGAAGTATAGGCATCTGGCAGGTTGAACTCCG 5 0.125 No Hit GTCGGAGGTGATATCGCACCTGCTCCAAGCCCCTCTTGCGCCAACAACTA 5 0.125 No Hit GGGCGCTTGGTCATGCTCTGAGCAATCTTGGACCAGAAGACTGTCATGGG 5 0.125 No Hit CCTTCATTCTGTGCTATGTATGTACGATAGTATATTCATTCACCAATGTG 5 0.125 No Hit GCCCTTTCCGGCGGTCTTGTGAGATTTCGTCACCTCCACCTTCTTTGCTC 5 0.125 No Hit GTTGGCGACGGGGTCATCAAGGTGGTCGAAGAGGTTCTCCAAGGGCCCCT 5 0.125 No Hit CTCTTCTTTCAGTGCTGACAGCCTCAAATCCAGTGCCCGAAGCAATTCAT 5 0.125 No Hit GGACCAGGTGCAGAGTGGACTCCTTCTGGATGTTGTAGTCCGCAAGGGTC 5 0.125 No Hit GCTGCTGATACGCGCTGTTCTCGCGGTGGTAGCGATCCTTGTAACGGCTG 5 0.125 No Hit GGCCTTGAGCGGCTCGAAGACATCCGGCTTCTTGTCGTAGACCTCGGTCT 5 0.125 No Hit CCCTTGTGGGTGGCGGTGGCCATGACCACGGTGGTCATCGTGGCGCCGGC 5 0.125 No Hit CAGGTCAACAGCATCGCCTTGCTTATAAAAGCCTATGGATGGAGAGAAGT 5 0.125 No Hit CGATGAGGTTGAGGAGGATGGCTGACACCTGCACGATGATGGACGCCATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0125 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.35 0.0 0.0 0.0 0.0 82-83 0.4375 0.0 0.0 0.0 0.0 84-85 0.725 0.0 0.0 0.0 0.0 86-87 0.8625 0.0 0.0 0.0 0.0 88-89 1.0375 0.0 0.0 0.0 0.0 90-91 1.2125 0.0 0.0 0.0 0.0 92-93 1.4125 0.0 0.0 0.0 0.0 94-95 1.7625000000000002 0.0 0.0 0.0 0.0 96-97 2.0125 0.0 0.0 0.0 0.0 98-99 2.3875 0.0 0.0 0.0 0.0 100-101 2.8125 0.0 0.0 0.0 0.0 102-103 3.0875 0.0 0.0 0.0 0.0 104-105 3.3625 0.0 0.0 0.0 0.0 106-107 3.7625 0.0 0.0 0.0 0.0 108-109 4.225 0.0 0.0 0.0 0.0 110-111 4.65 0.0 0.0 0.0 0.0 112-113 4.9625 0.0 0.0 0.0 0.0 114-115 5.6 0.0 0.0 0.0 0.0 116-117 6.1875 0.0 0.0 0.0 0.0 118-119 6.675000000000001 0.0 0.0 0.0 0.0 120-121 7.3 0.0 0.0 0.0 0.0 122-123 7.9125 0.0 0.0 0.0 0.0 124-125 8.412500000000001 0.0 0.0 0.0 0.0 126-127 9.037500000000001 0.0 0.0 0.0 0.0 128-129 9.45 0.0 0.0 0.0 0.0 130-131 9.925 0.0 0.0 0.0 0.0 132-133 10.55 0.0 0.0 0.0 0.0 134-135 11.2 0.0 0.0 0.0 0.0 136-137 11.9 0.0 0.0 0.0 0.0 138-139 12.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCTA 10 0.006830828 145.0 8 GGCAGAA 10 0.006830828 145.0 1 >>END_MODULE SRR13165367 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165367_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.605 37.0 37.0 37.0 37.0 37.0 2 35.9485 37.0 37.0 37.0 37.0 37.0 3 35.9095 37.0 37.0 37.0 37.0 37.0 4 35.964 37.0 37.0 37.0 37.0 37.0 5 36.147 37.0 37.0 37.0 37.0 37.0 6 36.0655 37.0 37.0 37.0 37.0 37.0 7 36.0275 37.0 37.0 37.0 37.0 37.0 8 36.06 37.0 37.0 37.0 37.0 37.0 9 36.0915 37.0 37.0 37.0 37.0 37.0 10-14 36.0286 37.0 37.0 37.0 37.0 37.0 15-19 35.980599999999995 37.0 37.0 37.0 37.0 37.0 20-24 35.96055 37.0 37.0 37.0 37.0 37.0 25-29 35.88615 37.0 37.0 37.0 37.0 37.0 30-34 35.89885 37.0 37.0 37.0 37.0 37.0 35-39 35.89815 37.0 37.0 37.0 37.0 37.0 40-44 35.87165 37.0 37.0 37.0 37.0 37.0 45-49 35.872699999999995 37.0 37.0 37.0 37.0 37.0 50-54 35.82645 37.0 37.0 37.0 37.0 37.0 55-59 35.78005 37.0 37.0 37.0 37.0 37.0 60-64 35.73805 37.0 37.0 37.0 37.0 37.0 65-69 35.688399999999994 37.0 37.0 37.0 37.0 37.0 70-74 35.626450000000006 37.0 37.0 37.0 37.0 37.0 75-79 35.66205 37.0 37.0 37.0 37.0 37.0 80-84 35.6531 37.0 37.0 37.0 37.0 37.0 85-89 35.608000000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.58695 37.0 37.0 37.0 37.0 37.0 95-99 35.54615 37.0 37.0 37.0 37.0 37.0 100-104 35.53025 37.0 37.0 37.0 37.0 37.0 105-109 35.50555000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.47935 37.0 37.0 37.0 37.0 37.0 115-119 35.365750000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.38315 37.0 37.0 37.0 37.0 37.0 125-129 35.347500000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.21145 37.0 37.0 37.0 34.6 37.0 135-139 35.197199999999995 37.0 37.0 37.0 32.2 37.0 140-144 35.09295 37.0 37.0 37.0 27.4 37.0 145-149 34.994150000000005 37.0 37.0 37.0 27.4 37.0 150-151 34.6435 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 5.0 14 10.0 15 9.0 16 6.0 17 5.0 18 2.0 19 2.0 20 6.0 21 9.0 22 11.0 23 4.0 24 13.0 25 9.0 26 10.0 27 12.0 28 15.0 29 22.0 30 35.0 31 36.0 32 70.0 33 86.0 34 184.0 35 572.0 36 2603.0 37 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.157523905385005 20.130850528434827 6.089582284851535 22.622043281328637 2 31.025000000000002 22.900000000000002 24.725 21.349999999999998 3 24.7 24.15 28.65 22.5 4 27.625 29.575000000000003 20.075000000000003 22.725 5 28.075 32.275 17.375 22.275 6 23.9 34.675 18.025 23.400000000000002 7 24.125 20.325 30.4 25.15 8 24.45 22.225 23.575 29.75 9 26.224999999999998 20.9 24.85 28.025 10-14 26.484999999999996 25.365 22.605 25.545 15-19 26.490000000000002 24.505 22.91 26.095000000000002 20-24 26.769723347841314 24.613537445595078 22.872579918955427 25.744159287608188 25-29 26.314736052039027 24.75356517388041 22.727045283962973 26.20465349011759 30-34 26.07695001751138 25.296442687747035 22.664732075849304 25.961875218892278 35-39 26.564610535794685 24.933713542448345 23.147731252188702 25.353944669568264 40-44 26.43718416971031 24.605993896032423 22.60969630259669 26.347125631660578 45-49 26.295777466479887 24.824894936962178 22.743646187712628 26.135681408845308 50-54 26.514583020661362 24.29836410025514 23.387863324828658 25.79918955425484 55-59 26.95521641230923 24.08806604953715 22.626970227670753 26.32974731048286 60-64 26.499574766121363 24.303366851768473 23.84811646405523 25.34894191805493 65-69 26.55062024809924 24.52981192476991 22.40396158463385 26.515606242496997 70-74 27.24543407555667 24.403302476857643 22.942206654991242 25.40905679259445 75-79 26.867463851503476 23.810476809926453 23.295141842197427 26.026917496372644 80-84 26.29814907453727 24.192096048024013 23.52176088044022 25.987993996998497 85-89 27.279095366756728 23.82667867507255 23.241268888221754 25.65295706994896 90-94 26.409525238881386 24.873680524288357 22.90259642803542 25.814197808794837 95-99 27.375056280954524 23.788083445895243 23.467907349041973 25.368952924108264 100-104 27.6257192894671 24.423317488116087 22.60695521641231 25.344008006004504 105-109 26.599949962471854 24.883662747060296 22.807105328996748 25.709281961471103 110-114 27.73025163840112 23.968182500375207 22.917604682575416 25.383961178648256 115-119 28.461346009507132 24.75856892669502 22.386790092569427 24.39329497122842 120-124 28.381285964473356 25.103827870903178 22.526895171378534 23.987990993244935 125-129 27.56429500650455 25.48283798659061 22.38066646652657 24.572200540378265 130-134 28.579292398538758 24.520842716308863 22.764349697242654 24.13551518790972 135-139 29.828863090472378 24.814851881505202 22.062650120096077 23.293634907926343 140-144 29.76136875281405 24.898694281855022 22.457351543348842 22.88258542198209 145-149 31.05829372029022 24.428321240930696 21.260945709281962 23.252439329497125 150-151 31.006006006006004 25.513013013013015 21.50900900900901 21.97197197197197 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 1.5 9 2.5 10 1.0 11 1.5 12 2.5 13 1.5 14 0.5 15 0.0 16 1.0 17 1.0 18 1.0 19 2.0 20 1.5 21 1.0 22 1.0 23 0.5 24 1.0 25 2.0 26 2.0 27 1.5 28 1.0 29 2.5 30 5.5 31 10.5 32 14.5 33 17.0 34 22.5 35 26.0 36 34.0 37 46.5 38 66.0 39 78.0 40 84.5 41 107.5 42 122.0 43 128.0 44 145.0 45 161.5 46 164.5 47 154.5 48 133.5 49 143.5 50 136.5 51 122.0 52 149.0 53 139.5 54 126.5 55 121.0 56 93.0 57 84.5 58 104.5 59 98.5 60 82.0 61 85.5 62 86.0 63 103.5 64 94.0 65 69.5 66 61.0 67 63.0 68 68.0 69 60.0 70 60.5 71 60.0 72 52.5 73 46.0 74 37.0 75 22.5 76 13.5 77 15.0 78 12.5 79 5.5 80 4.0 81 2.5 82 2.0 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 1.5 97 1.0 98 0.5 99 1.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.65 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.075 30-34 0.065 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.04 70-74 0.075 75-79 0.065 80-84 0.05 85-89 0.06999999999999999 90-94 0.055 95-99 0.055 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.08499999999999999 135-139 0.08 140-144 0.055 145-149 0.075 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.15 #Duplication Level Percentage of deduplicated Percentage of total 1 73.47153900210822 52.275000000000006 2 17.673928320449754 25.15 3 6.184118060435699 13.200000000000001 4 1.510892480674631 4.3 5 0.5621925509486999 2.0 6 0.28109627547434995 1.2 7 0.14054813773717498 0.7000000000000001 8 0.035137034434293744 0.2 9 0.035137034434293744 0.22499999999999998 >10 0.10541110330288123 0.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 10 0.25 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 10 0.25 No Hit GCCTCTTCTCGCTTGCTCTACCTGCTGCTTGCAACCATGGCACCCACCGT 10 0.25 No Hit GAGAAGAATTACTGGATAAATTAGCTGTTGGACTTGAAGAGCTTCAACGA 9 0.22499999999999998 No Hit GCTCATCATCTTGTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATT 8 0.2 No Hit AACGGATTGGCCATGACAGGAATGTCCTTTTTCAAGGCAGTTGCTCCTGC 7 0.17500000000000002 No Hit GCCGAAAGGATTCCTCCCCCTCTGCCATGGAAGAGGACTGCAGCAATTCA 7 0.17500000000000002 No Hit GGCGAGTCACTGCTCTGCAGCAGCGTCTGCTTGATGGCATCCGTGGAAAG 7 0.17500000000000002 No Hit GGCACCACCAGTTTGCAGGGTGATGTGAAATTCGCTGAGGTACTTGAGAT 7 0.17500000000000002 No Hit GAAAAACTTGCTGTTGCAGAGGAACTATGGCCCCTTGCTATTTCAGAACT 6 0.15 No Hit TGAAGCTGATGTACCGTGCACCCGTAACATCTAGCTATCACCAGAGCACA 6 0.15 No Hit GTTTGGCCACGCTGGGGCGAAGAGTGGTGGTGAGTTGGAATCAGCGCAAG 6 0.15 No Hit AAGAAAAGGAATGTTGAGTTACCCAAGGACACAAGTGAACTGAAACAGAT 6 0.15 No Hit GAAACATCCTTAACTGAGCTCCTCACTCACTCACTGCAGCTAGCCTCTTC 6 0.15 No Hit GTTTACTCTTCATATCTTGCGCTGGACCTGGATGAGGTGGAACCTTGCAT 6 0.15 No Hit CTGATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAA 6 0.15 No Hit GGCAATCTCGTCTTCTCCAATCGCCTCGAAGCTCGTCGAATCCTCCCCCA 6 0.15 No Hit GTTCTTGTCCAAGTGAAGTGAGCACCACTGCTGCAGAGAGAGAGATCGAG 5 0.125 No Hit AGAAGAAGAGCACGTCGTCTCCGCCGCCGTCTGCTGCCGCGTCGGCCACC 5 0.125 No Hit TATAGTACCTGACAAAGACCATGCGAAACTGCAAGTGGAGGTGAAGAGCA 5 0.125 No Hit GTGGCATCGGATTGTTGGTGGAGTGGGTTAGAGTCCCTCGAGTTCGTGGT 5 0.125 No Hit ATTACGTTTTTTGTGATGCTGATAGGTAGGGTGACATGTATTTTAGTAGC 5 0.125 No Hit CTGAGGCTGGGATCAAGTACATCCCCAGCAACACCTTCTCATACTATGAC 5 0.125 No Hit AAGTGGGCTGCTAGAGGTGGTTGCGAAGACCATTAAATCATGCCTTGATG 5 0.125 No Hit CAGAAACTGATGCCTCCGCCGTCCGATCTTCCCAAGTGTTAGAAATCTAA 5 0.125 No Hit AAGTGCTAACACGGGCAGACAGACGGGGGATACACGACATGACGAGCCCC 5 0.125 No Hit GCCAACTGGTGCTACGCAACCGTCGCGCCCCGCGCTAAGAGCGTCGTCGT 5 0.125 No Hit CAACTCTCCCCAATCCTCCACTCGCCTCAGAGCAATGGCGTCCATCATCG 5 0.125 No Hit TACAGGAAGCAGCACAAGAAGGATATCCACGCTGAGGCTGCCAAGAAAAG 5 0.125 No Hit GAATTTTACAGGAGATGTCTCAACTGGAGGAAACTAGGAAGTTTCATGAT 5 0.125 No Hit CAAGATCCAGGAGGAGCTTGACATTGATGTGCTAGTGCATGGAGAGCCTG 5 0.125 No Hit GCAGGTGCTCCTCATGGGCCTCGTCGAAGGGTTCCGCATCAACGGGCTCG 5 0.125 No Hit GGAAGGCAGAACTAATCGAGAGCTTGAAGGCCCTTGCCCACGGCCACCCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0125 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.037500000000000006 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.44999999999999996 0.0 0.0 0.0 0.0 84-85 0.725 0.0 0.0 0.0 0.0 86-87 0.8625 0.0 0.0 0.0 0.0 88-89 1.0375 0.0 0.0 0.0 0.0 90-91 1.2125 0.0 0.0 0.0 0.0 92-93 1.4 0.0 0.0 0.0 0.0 94-95 1.7374999999999998 0.0 0.0 0.0 0.0 96-97 1.9874999999999998 0.0 0.0 0.0 0.0 98-99 2.3625 0.0 0.0 0.0 0.0 100-101 2.7875 0.0 0.0 0.0 0.0 102-103 3.0625 0.0 0.0 0.0 0.0 104-105 3.3375000000000004 0.0 0.0 0.0 0.0 106-107 3.7375 0.0 0.0 0.0 0.0 108-109 4.225 0.0 0.0 0.0 0.0 110-111 4.65 0.0 0.0 0.0 0.0 112-113 4.9625 0.0 0.0 0.0 0.0 114-115 5.5875 0.0 0.0 0.0 0.0 116-117 6.15 0.0 0.0 0.0 0.0 118-119 6.675000000000001 0.0 0.0 0.0 0.0 120-121 7.2875 0.0 0.0 0.0 0.0 122-123 7.8875 0.0 0.0 0.0 0.0 124-125 8.3875 0.0 0.0 0.0 0.0 126-127 9.0125 0.0 0.0 0.0 0.0 128-129 9.425 0.0 0.0 0.0 0.0 130-131 9.9 0.0 0.0 0.0 0.0 132-133 10.524999999999999 0.0 0.0 0.0 0.0 134-135 11.2 0.0 0.0 0.0 0.0 136-137 11.9 0.0 0.0 0.0 0.0 138-139 12.600000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACCGA 10 0.007070461 143.3375 4 GGGGGGG 75 0.001227009 19.353588 145 >>END_MODULE Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984578 spots for SRR13165367.sra Written 1984578 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra Read 1984561 spots for SRR13165367.sra Written 1984561 spots for SRR13165367.sra SRR ids: ['SRR13165367.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_e195b3s0 SRR13165367.sra spots: 39691237 blocks: [[1, 1984561], [1984562, 3969122], [3969123, 5953683], [5953684, 7938244], [7938245, 9922805], [9922806, 11907366], [11907367, 13891927], [13891928, 15876488], [15876489, 17861049], [17861050, 19845610], [19845611, 21830171], [21830172, 23814732], [23814733, 25799293], [25799294, 27783854], [27783855, 29768415], [29768416, 31752976], [31752977, 33737537], [33737538, 35722098], [35722099, 37706659], [37706660, 39691237]] SRR13165367 file size 13467118 SRR13165367 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165367 SRR13165367_1.fastq SRR13165367_2.fastq Input file: SRR13165367_1.fastq Paired file: SRR13165367_2.fastq trimmed: SRR13165367-trimmed-pair1.fastq, SRR13165367-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:21:06 2024 >> started Sat Dec 7 16:28:59 2024 >> done (472.667s) 39691237 read pairs processed; of these: 996 ( 0.00%) short read pairs filtered out after trimming by size control 99827 ( 0.25%) empty read pairs filtered out after trimming by size control 39590414 (99.75%) read pairs available; of these: 6389823 (16.14%) trimmed read pairs available after processing 33200591 (83.86%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 71 0.00% 19 86 0.00% 20 93 0.00% 21 136 0.00% 22 179 0.00% 23 166 0.00% 24 233 0.00% 25 264 0.00% 26 295 0.00% 27 339 0.00% 28 347 0.00% 29 311 0.00% 30 364 0.00% 31 344 0.00% 32 395 0.00% 33 393 0.00% 34 382 0.00% 35 364 0.00% 36 416 0.00% 37 422 0.00% 38 426 0.00% 39 445 0.00% 40 422 0.00% 41 478 0.00% 42 517 0.00% 43 530 0.00% 44 592 0.00% 45 559 0.00% 46 580 0.00% 47 577 0.00% 48 765 0.00% 49 755 0.00% 50 943 0.00% 51 1084 0.00% 52 1109 0.00% 53 1162 0.00% 54 1256 0.00% 55 1316 0.00% 56 1329 0.00% 57 1663 0.00% 58 1838 0.00% 59 1894 0.00% 60 2356 0.01% 61 2543 0.01% 62 2813 0.01% 63 3339 0.01% 64 3767 0.01% 65 3646 0.01% 66 4129 0.01% 67 4492 0.01% 68 4918 0.01% 69 5804 0.01% 70 6241 0.02% 71 6996 0.02% 72 8311 0.02% 73 8994 0.02% 74 9939 0.03% 75 11084 0.03% 76 11709 0.03% 77 13006 0.03% 78 14287 0.04% 79 15674 0.04% 80 16724 0.04% 81 18554 0.05% 82 20656 0.05% 83 22599 0.06% 84 24998 0.06% 85 27548 0.07% 86 28627 0.07% 87 30587 0.08% 88 32747 0.08% 89 33723 0.09% 90 36896 0.09% 91 38891 0.10% 92 40985 0.10% 93 43660 0.11% 94 47307 0.12% 95 50041 0.13% 96 52932 0.13% 97 55371 0.14% 98 56554 0.14% 99 59796 0.15% 100 60657 0.15% 101 62907 0.16% 102 65224 0.16% 103 67720 0.17% 104 70171 0.18% 105 73252 0.19% 106 75687 0.19% 107 76080 0.19% 108 80348 0.20% 109 81852 0.21% 110 83163 0.21% 111 84152 0.21% 112 87379 0.22% 113 88552 0.22% 114 92422 0.23% 115 93592 0.24% 116 96158 0.24% 117 98989 0.25% 118 99088 0.25% 119 99726 0.25% 120 102611 0.26% 121 103824 0.26% 122 103601 0.26% 123 106970 0.27% 124 110426 0.28% 125 110007 0.28% 126 113293 0.29% 127 113086 0.29% 128 113564 0.29% 129 117445 0.30% 130 116819 0.30% 131 118431 0.30% 132 121447 0.31% 133 122626 0.31% 134 121964 0.31% 135 125908 0.32% 136 124689 0.31% 137 124903 0.32% 138 126599 0.32% 139 130386 0.33% 140 131143 0.33% 141 131774 0.33% 142 133721 0.34% 143 133692 0.34% 144 136769 0.35% 145 139877 0.35% 146 137676 0.35% 147 143825 0.36% 148 140294 0.35% 149 143818 0.36% 150 142432 0.36% 151 33200591 83.86% 39590414 reads passed initial QC criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=33 prefix-density=0.70 prefix-fanout=2.0 sequence=TAGGCGTCCGGGTACTCCTTCTTGACCTCCTCCAGCTCCTTGAGCACCTGTGTGGCGTCGGT criterion=fanout-score sequence-density=0.03 sequence-density-rank=33 fanout-score=380.70 fanout-score-rank=1 prefix-density=0.63 prefix-fanout=15.8 sequence=GGCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAG criterion=sequence-density sequence-density=0.56 sequence-density-rank=1 fanout-score=3.44 fanout-score-rank=12 prefix-density=0.61 prefix-fanout=3.2 sequence=GAGTTCAGCAAGGTCGGCTT criterion=fanout-score sequence-density=0.03 sequence-density-rank=27 fanout-score=71.01 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=8.3 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGATGTCTGCTACCTTCTCGTCCACCGTCGGAGCTCCGGCTTCTACGCCAACCAGCTTCCTTGGGAAGAAGCTCAAGAAGCAGGTGACCTCGGCCGTGAACTACCATGGCAAGAGCACCAAGGCCAACAGATTCACAGTCATGGCCAAGGAGGTGGACGAGTCAAAGCAGACTGACCAGGACAGGTGGAAGGGCCTCGCCTACGATATCTCCGACGACCAGCAGGACATCACCAGGGGGAAGGGTATCGTCGACTCGCTCTTCCAGGCGCCCATGGGCGACGGTACCCACGTGGCCGTCCTCAGCTCCCAAGAGTACATCAGCCAGGGCCTAAGGAAGTACGACTTCGACAACA SRR13165367 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:35:08 Started mapping on | Dec 07 16:35:09 Finished on | Dec 07 17:20:10 Mapping speed, Million of reads per hour | 52.77 Number of input reads | 39590414 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 37423403 Uniquely mapped reads % | 94.53% Average mapped length | 291.54 Number of splices: Total | 35650705 Number of splices: Annotated (sjdb) | 33376837 Number of splices: GT/AG | 35175598 Number of splices: GC/AG | 400077 Number of splices: AT/AC | 14739 Number of splices: Non-canonical | 60291 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.01% Deletion average length | 3.00 Insertion rate per base | 0.01% Insertion average length | 2.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 399763 % of reads mapped to multiple loci | 1.01% Number of reads mapped to too many loci | 75670 % of reads mapped to too many loci | 0.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.41% % of reads unmapped: other | 0.86% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1767682 1767682 1767682 N_multimapping 399763 399763 399763 N_noFeature 1182624 36412476 1515741 N_ambiguous 820088 5312 143417 UnstrandedReadsAssigned:35420691 PositiveStrandReadsAssigned:1005615 NegativeStrandReadsAssigned:35764245 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165367 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165367-trimmed-pair1.fastq SRR13165367-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 39,590,414 reads, 36,340,785 reads pseudoaligned [quant] estimated average fragment length: 252.494 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,230 rounds 52973 SRR13165367.ke.tsv 35125 SRR13165367.se.tsv 88098 total ==> SRR13165367.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 685.275 0 0 PNS24247 1044 792.506 81.5505 4.01573 PNS24249 1928 1676.51 234.656 5.46219 PNS24246 1044 792.506 81.5505 4.01573 PNS24248 1044 792.506 81.5505 4.01573 PNS24244 1471 1219.51 113.692 3.6382 PNS24243 293 106.173 0 0 KQK14069 1603 1351.51 747.73 21.5907 KQK14071 474 246.168 5.42287 0.859681 ==> SRR13165367.se.tsv <== BRADI_1g14170v3 781 BRADI_1g53295v3 112 BRADI_1g59795v3 684 BRADI_1g07683v3 0 BRADI_1g00485v3 5 BRADI_1g20270v3 758 BRADI_1g74790v3 500 BRADI_1g09890v3 0 BRADI_1g77505v3 458 BRADI_1g48960v3 0 SRR13165367 completed mapping pipeline successfully