Starting /dee2/code/volunteer_pipeline.sh SRR13165368 current disk space = 1541901602816 free memory = 1595965624 SRR13165368 SRAfilesize 6c630b9d34b051de79223de49c27c104 SRR13165368.sra SRR13165368.sra file validated SRR13165368 is paired end SRR13165368 is conventional basespace SRR13165368 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165368_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5735 37.0 37.0 37.0 37.0 37.0 2 36.067 37.0 37.0 37.0 37.0 37.0 3 36.539 37.0 37.0 37.0 37.0 37.0 4 36.557 37.0 37.0 37.0 37.0 37.0 5 36.605 37.0 37.0 37.0 37.0 37.0 6 36.5405 37.0 37.0 37.0 37.0 37.0 7 36.5405 37.0 37.0 37.0 37.0 37.0 8 36.5855 37.0 37.0 37.0 37.0 37.0 9 36.597 37.0 37.0 37.0 37.0 37.0 10-14 36.548 37.0 37.0 37.0 37.0 37.0 15-19 36.5297 37.0 37.0 37.0 37.0 37.0 20-24 36.4764 37.0 37.0 37.0 37.0 37.0 25-29 36.43829999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.392100000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.327600000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.33540000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.1178 37.0 37.0 37.0 37.0 37.0 50-54 36.33 37.0 37.0 37.0 37.0 37.0 55-59 36.1182 37.0 37.0 37.0 37.0 37.0 60-64 36.097699999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.9335 37.0 37.0 37.0 37.0 37.0 70-74 36.0932 37.0 37.0 37.0 37.0 37.0 75-79 36.17999999999999 37.0 37.0 37.0 37.0 37.0 80-84 36.1793 37.0 37.0 37.0 37.0 37.0 85-89 36.1584 37.0 37.0 37.0 37.0 37.0 90-94 36.159800000000004 37.0 37.0 37.0 37.0 37.0 95-99 36.0921 37.0 37.0 37.0 37.0 37.0 100-104 36.0695 37.0 37.0 37.0 37.0 37.0 105-109 36.097699999999996 37.0 37.0 37.0 37.0 37.0 110-114 36.025999999999996 37.0 37.0 37.0 37.0 37.0 115-119 36.0536 37.0 37.0 37.0 37.0 37.0 120-124 35.939800000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.9072 37.0 37.0 37.0 37.0 37.0 130-134 35.940599999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.8149 37.0 37.0 37.0 37.0 37.0 140-144 35.6802 37.0 37.0 37.0 37.0 37.0 145-149 35.50450000000001 37.0 37.0 37.0 37.0 37.0 150-151 35.239000000000004 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 1.0 21 1.0 22 1.0 23 0.0 24 1.0 25 4.0 26 7.0 27 12.0 28 16.0 29 30.0 30 31.0 31 27.0 32 61.0 33 76.0 34 188.0 35 330.0 36 2821.0 37 391.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.875 10.475 5.65 34.0 2 21.695257315842582 12.714429868819375 32.84561049445005 32.74470232088799 3 20.0 15.1 26.700000000000003 38.2 4 27.175 17.7 21.4 33.725 5 28.275 25.825 22.25 23.65 6 24.45 30.725 21.0 23.825 7 18.4 27.075 34.875 19.650000000000002 8 19.400000000000002 26.825 28.275 25.5 9 21.275 22.0 31.75 24.975 10-14 23.150000000000002 26.66 24.685000000000002 25.505 15-19 23.625 24.81 25.28 26.284999999999997 20-24 23.57 25.119999999999997 25.369999999999997 25.94 25-29 23.175 24.43 25.575 26.82 30-34 23.645 24.425 25.790000000000003 26.14 35-39 23.75 24.865000000000002 25.2 26.185000000000002 40-44 24.055 25.22 24.560000000000002 26.165 45-49 23.07 24.995 25.290000000000003 26.645000000000003 50-54 23.330000000000002 24.08 25.185000000000002 27.405 55-59 23.22 24.07 25.615 27.095000000000002 60-64 24.37 24.04 25.81 25.779999999999998 65-69 24.77 24.865000000000002 24.85 25.515 70-74 25.915 24.310000000000002 24.42 25.355 75-79 25.695 23.895 24.095 26.314999999999998 80-84 25.185000000000002 24.07 24.815 25.929999999999996 85-89 25.945 23.61 24.585 25.86 90-94 25.759999999999998 24.455 24.23 25.555 95-99 25.645 24.235 24.33 25.790000000000003 100-104 25.924999999999997 24.610000000000003 23.94 25.525 105-109 25.669999999999998 25.064999999999998 23.835 25.430000000000003 110-114 25.564999999999998 24.585 24.33 25.52 115-119 25.89 24.945 22.830000000000002 26.334999999999997 120-124 25.419999999999998 24.595 23.66 26.325 125-129 25.729999999999997 24.52 24.21 25.540000000000003 130-134 25.61 24.81 22.939999999999998 26.640000000000004 135-139 26.400000000000002 24.705 23.345 25.55 140-144 27.185 25.09 22.2 25.525 145-149 26.3 24.2 23.625 25.874999999999996 150-151 26.3125 22.8125 23.8625 27.0125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 0.5 25 1.0 26 1.0 27 1.0 28 2.0 29 2.5 30 2.0 31 2.5 32 7.0 33 17.0 34 31.5 35 33.5 36 43.0 37 59.0 38 66.0 39 84.5 40 108.0 41 130.0 42 155.5 43 163.0 44 172.5 45 183.0 46 184.5 47 183.0 48 153.0 49 148.5 50 163.0 51 158.0 52 150.5 53 130.0 54 121.5 55 121.5 56 127.0 57 107.5 58 84.5 59 94.0 60 78.0 61 69.5 62 62.0 63 51.0 64 64.0 65 73.5 66 69.0 67 59.0 68 39.5 69 28.5 70 35.0 71 30.0 72 22.5 73 22.5 74 22.5 75 20.0 76 14.5 77 14.0 78 11.5 79 6.5 80 5.0 81 2.5 82 0.5 83 0.5 84 0.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.8999999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.75 #Duplication Level Percentage of deduplicated Percentage of total 1 73.72759856630825 51.425 2 16.738351254480285 23.35 3 6.021505376344086 12.6 4 1.935483870967742 5.4 5 0.8960573476702508 3.125 6 0.2867383512544803 1.2 7 0.21505376344086022 1.05 8 0.07168458781362007 0.4 9 0.0 0.0 >10 0.10752688172043011 1.4500000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCAGGAGATCTCGTAT 31 0.775 TruSeq Adapter, Index 1 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCAGGAGATCGCGTAT 17 0.42500000000000004 TruSeq Adapter, Index 1 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCGGAGATCTCGTAT 10 0.25 TruSeq Adapter, Index 18 (97% over 38bp) CAGGCATAAGGTTTCGTTGTGAAGAATTCACTTTCAAGGGCAGAAGCCGC 8 0.2 No Hit GGAAATCTTAGCAACAGGATCATATTCAGGCATGAGGGCACCGACAAGGG 8 0.2 No Hit TCTTTCTCGAACCCAATTGTTGGGAACTGTTTCGCATATTCCTCCACATC 7 0.17500000000000002 No Hit GTCGATTCTGCCATCTCTTTGAGGAAGGAGACGACGTCTTCAGATGAGTT 7 0.17500000000000002 No Hit CAGACAAACAAGCATGAATACGCTGACTGACAGACACTACAGTACTACAC 7 0.17500000000000002 No Hit CTTAACACCAAAGAAGAGTTCGACCCAATGCTTTATTTCTGTCTTAGTGA 7 0.17500000000000002 No Hit GCCATCCTTCACGGCTCCCTCGACGTCCTGTGGTGACCCATAAAGCTCCT 7 0.17500000000000002 No Hit GTGGCTAGCTTGATCTTATGACATATACTCATTATAGTTATAAGCTAGAT 7 0.17500000000000002 No Hit GCCCTATCTAAATGTTCCTACAAGGAGTCCTATTAGTTAGTTAGGGACAG 6 0.15 No Hit CCTTGCTGAATGCTGCTCTCTACTAATTACTGGTAGAAGGAAAGAAGGAA 6 0.15 No Hit GACCAGGCAAATGTACATGAACTCAGTGAAAAAGCAGCTGAGTTTGATAC 6 0.15 No Hit CCGGTCAATGCGGCATTGAGCGCACTGTGAACATCCACTCCGATCTGAGC 6 0.15 No Hit AAGGGCGCAAATTAATGGAAAGAACCTCATTACTGTTCCCCAACAGCAGC 6 0.15 No Hit GGCACAATGTATAAAAAGTACAACATATTAGGTTTTAACCACACGATGCT 6 0.15 No Hit GCTTCCATGTATCTATATATTAGCTGGATGAGCATAACTCCATTCCCCTT 6 0.15 No Hit GGATTCCATTGCACATGGCCATGGCCTCGGCGGCGGCGGTGGCCTCGTCA 6 0.15 No Hit GTCTCGCGATCTCCATGTGCCATACAAATTGGAATGTCTGGGTACAAATG 5 0.125 No Hit CCAGAAACAAGAGCGCCAGCCAGAACACCAGAAAGAGTTTCCACACCAAA 5 0.125 No Hit ACGTCATCACTTGCTAGCTTACAGAAACATCGTATTATCAGGCAATTCAT 5 0.125 No Hit CGTGCTTAGGATGAGCGAGACGATTATACATATTTTACGCGATTACACGG 5 0.125 No Hit GGCTGTAGTTACATGAGTGTCCTTGTAATCCATAAGTTTGGCCAGTCCAA 5 0.125 No Hit GGCAAAAGGAGATTTGTACAATTCATTCTGAAGGTCCAAATAGAATTGCC 5 0.125 No Hit GTGCTAGCACCCTACTTGAGGCTAAAGAAGATAAGTAAAAGGTAATTGCA 5 0.125 No Hit GATCCGTCTGCTTGGATCAAACACCAGCATTCTCTCTAACAGATCAACTG 5 0.125 No Hit GTTCAAACAGAGAAAATAAGACAATGTCTAGAAGAGCAACCAAGTTTCCC 5 0.125 No Hit CAGCAACAGTTCAAATGCAGCAGTACTACTGCTACTGCTAGTTCTATTGA 5 0.125 No Hit GTGGGCTGAGAGTGAGCAAAACACATGCGGATCTGACACATCTGCAACGC 5 0.125 No Hit GTGACAATCGCCCTATGAAGACTCGCTTTCGCTACGGCTCCGGTGGGTTC 5 0.125 No Hit CGTCGCGCGAGATCCGGAGCCCGTGCGGCCGCCGCAGCAGCAAGGGGGAG 5 0.125 No Hit GCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTG 5 0.125 No Hit TGCAGATACTTCCCTTGTTCTTCAATTCGCAACTGCAATTTTCTCTGAGT 5 0.125 No Hit CTCCCATCCTGATGCCTCCTGGTACCATAGCTGAAACATCACCAGGAACT 5 0.125 No Hit GGGCCAAGAAGTGTCCCTACAATACGTCCATATGTAGCCCCAGCAAGTAT 5 0.125 No Hit GTTCGGTTTCTGTTTGTGCTCCCACCTGAATGACAGCAACACCACCAGCA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCGGAGATCGCGTAT 5 0.125 TruSeq Adapter, Index 18 (97% over 38bp) CAGGTTCCAGCTTCAGATACCATGGATAATAGCCTAACAGGAATCATAAT 5 0.125 No Hit GTGGGAATTCAGCTGGAGAAGTAGGTCCCAGACTGACTAGGGGTCTGGAT 5 0.125 No Hit CTCTGCATAACAATGAGCGACTTTTGCATACTTTCTGTAAGCTCCTGATC 5 0.125 No Hit CCCGCCGTGGCGTTCTGCGCGGCGGAAGCGACGCACGCCATGCTGGCGGC 5 0.125 No Hit GTTGGATGATCTTGTTGGAACATCTCGATTCTTCCGTGCTTTCTTCGATG 5 0.125 No Hit CTTATCTGCAAAGCACGTGTGCCCAGGATGCGTGCACGCTCATACTTAGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0125 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.16249999999999998 0.0 0.0 0.0 0.0 62-63 0.225 0.0 0.0 0.0 0.0 64-65 0.25 0.0 0.0 0.0 0.0 66-67 0.3 0.0 0.0 0.0 0.0 68-69 0.325 0.0 0.0 0.0 0.0 70-71 0.3625 0.0 0.0 0.0 0.0 72-73 0.425 0.0 0.0 0.0 0.0 74-75 0.5125 0.0 0.0 0.0 0.0 76-77 0.625 0.0 0.0 0.0 0.0 78-79 0.8375 0.0 0.0 0.0 0.0 80-81 0.9 0.0 0.0 0.0 0.0 82-83 0.975 0.0 0.0 0.0 0.0 84-85 1.0375 0.0 0.0 0.0 0.0 86-87 1.275 0.0 0.0 0.0 0.0 88-89 1.6375 0.0 0.0 0.0 0.0 90-91 1.9875 0.0 0.0 0.0 0.0 92-93 2.4000000000000004 0.0 0.0 0.0 0.0 94-95 2.6500000000000004 0.0 0.0 0.0 0.0 96-97 3.0875 0.0 0.0 0.0 0.0 98-99 3.4375 0.0 0.0 0.0 0.0 100-101 3.7125 0.0 0.0 0.0 0.0 102-103 4.300000000000001 0.0 0.0 0.0 0.0 104-105 5.1625 0.0 0.0 0.0 0.0 106-107 6.025 0.0 0.0 0.0 0.05 108-109 6.5875 0.0 0.0 0.0 0.05 110-111 7.137499999999999 0.0 0.0 0.0 0.05 112-113 7.762499999999999 0.0 0.0 0.0 0.05 114-115 8.325 0.0 0.0 0.0 0.05 116-117 9.0625 0.0 0.0 0.0 0.05 118-119 9.625 0.0 0.0 0.0 0.05 120-121 10.3375 0.0 0.0 0.0 0.05 122-123 10.9125 0.0 0.0 0.0 0.05 124-125 11.75 0.0 0.0 0.0 0.05 126-127 12.7625 0.0 0.0 0.0 0.05 128-129 13.4875 0.0 0.0 0.0 0.05 130-131 14.399999999999999 0.0 0.0 0.0 0.05 132-133 15.212499999999999 0.0 0.0 0.0 0.05 134-135 16.2125 0.0 0.0 0.0 0.05 136-137 17.2125 0.0 0.0 0.0 0.05 138-139 18.0375 0.0 0.0 0.0 0.05 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGAAC 10 0.006830828 145.0 1 ATAATAA 10 0.006830828 145.0 5 TCTGCCA 10 0.006830828 145.0 7 GTCCATA 10 0.006830828 145.0 1 CCATAAT 10 0.006830828 145.0 3 TAATAAG 10 0.006830828 145.0 6 CTGCCAT 10 0.006830828 145.0 8 AATAAGG 10 0.006830828 145.0 7 CATAATA 10 0.006830828 145.0 4 TAAGGCG 10 0.006830828 145.0 9 ATAAGGC 20 3.5877043E-4 108.75 8 >>END_MODULE SRR13165368 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165368_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.01425 37.0 37.0 37.0 37.0 37.0 2 36.144 37.0 37.0 37.0 37.0 37.0 3 36.0445 37.0 37.0 37.0 37.0 37.0 4 36.1455 37.0 37.0 37.0 37.0 37.0 5 36.176 37.0 37.0 37.0 37.0 37.0 6 36.218 37.0 37.0 37.0 37.0 37.0 7 36.1615 37.0 37.0 37.0 37.0 37.0 8 36.098 37.0 37.0 37.0 37.0 37.0 9 35.9965 37.0 37.0 37.0 37.0 37.0 10-14 36.016099999999994 37.0 37.0 37.0 37.0 37.0 15-19 35.963100000000004 37.0 37.0 37.0 37.0 37.0 20-24 35.96445 37.0 37.0 37.0 37.0 37.0 25-29 35.77785 37.0 37.0 37.0 37.0 37.0 30-34 35.69945 37.0 37.0 37.0 37.0 37.0 35-39 35.65285 37.0 37.0 37.0 37.0 37.0 40-44 35.643150000000006 37.0 37.0 37.0 37.0 37.0 45-49 35.5687 37.0 37.0 37.0 37.0 37.0 50-54 35.59745 37.0 37.0 37.0 37.0 37.0 55-59 35.71385 37.0 37.0 37.0 37.0 37.0 60-64 35.73245000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.60745 37.0 37.0 37.0 37.0 37.0 70-74 35.48175 37.0 37.0 37.0 37.0 37.0 75-79 35.45885 37.0 37.0 37.0 37.0 37.0 80-84 35.56849999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.606849999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.65965 37.0 37.0 37.0 37.0 37.0 95-99 35.65715 37.0 37.0 37.0 37.0 37.0 100-104 35.66165 37.0 37.0 37.0 37.0 37.0 105-109 35.634550000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.54055 37.0 37.0 37.0 37.0 37.0 115-119 35.53245 37.0 37.0 37.0 37.0 37.0 120-124 35.43805 37.0 37.0 37.0 37.0 37.0 125-129 35.433299999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.27145 37.0 37.0 37.0 34.6 37.0 135-139 35.10360000000001 37.0 37.0 37.0 29.8 37.0 140-144 34.950250000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.7268 37.0 37.0 37.0 25.0 37.0 150-151 34.36575 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 7.0 14 6.0 15 4.0 16 3.0 17 5.0 18 4.0 19 6.0 20 7.0 21 9.0 22 8.0 23 5.0 24 9.0 25 12.0 26 19.0 27 21.0 28 31.0 29 25.0 30 32.0 31 37.0 32 69.0 33 95.0 34 198.0 35 541.0 36 2609.0 37 236.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.4556198139301 18.883580588383204 9.303495096806639 27.35730450088006 2 31.55 23.375 23.925 21.15 3 24.4 24.224999999999998 27.975 23.400000000000002 4 30.85 28.725 18.95 21.475 5 30.725 30.425 19.55 19.3 6 26.55 34.599999999999994 17.525 21.325 7 25.45 19.425 31.75 23.375 8 24.775 23.849999999999998 23.9 27.474999999999998 9 25.650000000000002 23.65 26.625 24.075 10-14 28.110000000000003 26.145000000000003 21.38 24.365000000000002 15-19 28.005000000000003 25.09 22.7 24.205 20-24 29.19105508029416 24.263344839661812 22.27725248886888 24.268347591175147 25-29 28.111083312484364 25.098824118088565 23.047285464098074 23.742807105328996 30-34 27.437834592485117 24.73107519887927 23.475258918296895 24.35583129033872 35-39 27.980389214067735 23.768072439841916 24.06323477912852 24.18830356696183 40-44 28.033221594036124 24.06564266773403 22.92490118577075 24.976234552459097 45-49 27.376425855513308 24.484690814488694 24.06443866319792 24.07444466680008 50-54 27.660213117214465 24.413427385061784 23.617989894441944 24.308369603281804 55-59 28.271203402551915 24.048036027020263 22.8771578684013 24.80360270202652 60-64 28.125469007954372 24.698584221321727 22.977637700735404 24.198309069988493 65-69 27.209523333166608 24.95373380683239 23.403191116890913 24.433551743110087 70-74 28.361270953214913 24.763572679509632 23.312484363272453 23.562672004003 75-79 27.587932155901335 24.87616951018162 23.50027517886626 24.035623155050782 80-84 28.134067033516757 24.807403701850923 23.121560780390197 23.936968484242122 85-89 28.13109832374281 25.449086815111333 23.072304228171127 23.34751063297473 90-94 28.105458001901045 24.093251288208513 23.60798439141528 24.19330631847516 95-99 27.885336935314424 25.103807093901647 23.307819300615336 23.703036670168594 100-104 28.56642481861396 25.033775331498624 22.586940205153866 23.81285964473355 105-109 28.701526144608458 24.33324993745309 23.037277958468852 23.9279459594696 110-114 28.955925759167545 24.958727300015006 23.22777527640202 22.85757166441543 115-119 30.047535651738805 25.364023017262948 22.852139104328245 21.736302226670002 120-124 30.29271953965474 24.798598949211907 23.252439329497125 21.656242181636227 125-129 30.47132993095167 24.772340638446913 23.151205844090864 21.605123586510558 130-134 31.716959415503176 24.89115748386128 22.16884351698944 21.2230395836461 135-139 30.76961569255404 24.284427542033626 23.37369895916733 21.572257806244995 140-144 33.02816549102006 25.013757566661667 21.19665816198909 20.76141878032918 145-149 34.25740592473979 23.45876701361089 21.757405924739793 20.52642113690953 150-151 34.12162162162162 23.1981981981982 22.284784784784783 20.395395395395397 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 1.0 20 1.0 21 0.5 22 0.5 23 1.0 24 1.5 25 1.5 26 2.0 27 3.5 28 5.0 29 7.5 30 7.0 31 4.0 32 3.5 33 7.0 34 18.0 35 33.0 36 32.5 37 45.5 38 60.5 39 84.5 40 119.0 41 125.5 42 132.5 43 145.0 44 147.0 45 171.0 46 188.5 47 167.5 48 168.5 49 185.0 50 168.0 51 146.5 52 130.0 53 106.0 54 107.0 55 113.0 56 112.0 57 109.0 58 89.5 59 73.0 60 72.5 61 73.5 62 76.0 63 70.5 64 60.0 65 54.0 66 56.5 67 58.5 68 62.5 69 60.5 70 43.0 71 38.0 72 32.0 73 26.0 74 29.5 75 26.0 76 19.5 77 10.5 78 5.0 79 4.0 80 5.5 81 4.5 82 3.0 83 2.5 84 0.0 85 1.5 86 2.0 87 1.0 88 0.5 89 1.0 90 1.5 91 2.0 92 3.0 93 4.0 94 5.0 95 6.5 96 8.5 97 7.5 98 9.0 99 10.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.575 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.075 30-34 0.065 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.034999999999999996 70-74 0.075 75-79 0.065 80-84 0.05 85-89 0.075 90-94 0.055 95-99 0.055 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.08499999999999999 135-139 0.08 140-144 0.055 145-149 0.08 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.3 #Duplication Level Percentage of deduplicated Percentage of total 1 74.82468443197756 53.349999999999994 2 15.918653576437588 22.7 3 5.925666199158485 12.675 4 1.9284712482468442 5.5 5 0.876577840112202 3.125 6 0.24544179523141654 1.05 7 0.21037868162692847 1.05 8 0.03506311360448808 0.2 9 0.0 0.0 >10 0.03506311360448808 0.35000000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 14 0.35000000000000003 No Hit GTTTGATCGACAGGTCACCGTCGACCGTCCAGATGTCGCTGGTCGTGTCA 8 0.2 No Hit GAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAA 7 0.17500000000000002 No Hit CAGGAGGATGTACCCCTGATTAATATTATAAACTAATACATACGTGGCCC 7 0.17500000000000002 No Hit GCCAATTGGTCTCTTCATAAAGCGGTCATCAATCATCTTCCGCTCAGACT 7 0.17500000000000002 No Hit CCGCCATCGGCCTGCTCATCGTCAGCAGCTACTGATCCAACCAAGCACAA 7 0.17500000000000002 No Hit CAAGGACACAGAAATCACAGGTCGCACCGGAGAGGTCGTCTTCTTCCAGC 7 0.17500000000000002 No Hit AACTCGAATTCTTATCGATACTGGAACTCAGAGCATAGGAGGGAAAGTCG 7 0.17500000000000002 No Hit TGGTAAGAGTGGATTAAACAAGTCCAGAGTCCTACAATCCCCAGGCCTTC 6 0.15 No Hit TGTGGAGGAAGACTTTGCCAAGGTTGCTGACTTCTTTGATTCAGCAGTGA 6 0.15 No Hit CGTATGTTGTTAAAAGAAGGCTCAGAAGGTTTCTGTCATCAGGACCCACT 6 0.15 No Hit GGAGGAGGATCCGGTGGTAATGGCGGTGGTTATCCTGGAGGTGGATATGG 6 0.15 No Hit GTTCAAGATGGGCGTCCGCATCGGCAGGCCCCTGAGCAAGGACGTCGTGC 6 0.15 No Hit AAGTGGTGCTGTTGAGCTTTTGGGCGGAGGTTCACTACAACTCGATTTTT 6 0.15 No Hit GCCGTCTCGAGTCGCTGCTCAATTACCAGACCATGGTCGCCGACCTCACC 6 0.15 No Hit ATTCAACAACTAAAATTGATCACTGAGTTGATAGGGTCACCAGATGATTC 5 0.125 No Hit GCCTGTTGAAGAATGAGCCGGCGACTCATAGGCAGTGGCTTGGTTAAGGG 5 0.125 No Hit TGGAGGAGTGCCCTACCATTGGTCGTTCTGGAAATTTTAAGAACTTCCAG 5 0.125 No Hit GCGGAACCAGCGGCGGCGGGCGCCATGGCGGACGATGATTACAATGAAGT 5 0.125 No Hit GGGGAACGGACGAGGAGAAGATCGGGAAAAACGGGGTCACAAGAATTTTA 5 0.125 No Hit GCCTGGTGATGGCAAGTTCATTGTCAACGACAAGCAGTTTGATGTCTACT 5 0.125 No Hit GGTGTATTTGTTCAATGGTGACTCTGGACAGTTGTCTGTTTTGAATAGGC 5 0.125 No Hit TGTGGATAAGAAGATAGATAAATAATGGATGCACAAGACACTCCTATGAC 5 0.125 No Hit CACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAA 5 0.125 No Hit CTTACTGAAGCCCTGCGCCTTCAGATGGAGGTTCAGAAACGTCTTCATGA 5 0.125 No Hit CCTGCGTAACAGGAGCAGAAGGAGAACTCGGCAATACATCATCATTGGAG 5 0.125 No Hit GGTGGGAGCACCTCGAGAAAGGCACGCTTCAAGAGGCCGTGGACATGGAG 5 0.125 No Hit TTTGTCACTAATTTCGTTCATTATTGTATTAAATTTAGCTTTTCCACTCG 5 0.125 No Hit TGTTTCCACAATCGCAGAGCTGAAGCCTGGTGTTCTATCTGTACATGAGG 5 0.125 No Hit GAACAGCATTCCCCAACGGCCGCAGCCGCCGCTCCCTGCCTTCTCTCCAA 5 0.125 No Hit AGTACAAGTACCTTAAGTCAGCCCTGAAGGTGGCTTTGAAAGGGAAATGA 5 0.125 No Hit GTTGATTGTTGGAGCTATGCTCCCATACTGGTTCTCTGCCATGACCATGA 5 0.125 No Hit GCGCTCTCCGGCCCCAACTACGGCGTGTCCGCCCGCACGGGGCTCTTCCT 5 0.125 No Hit GGAAAGACGGACAGCATGAGGGTGGAGATCAAGAGCCGGCAGGCGCAGAA 5 0.125 No Hit GGGGACCATATTTTCACAGATGTAAGCCAATCGAAAGTTCATTTAAGATG 5 0.125 No Hit CAAGAATAAGGGGATAGATGGTTCAAGAGTGGAGAAGGTTTTAGAAAATG 5 0.125 No Hit GGAATAAACAGTGCCAAGATTGGTGGTGAGGGTGGTGAGGTTAAGTTGGC 5 0.125 No Hit GAGGTGGGCCTGTCACTTGACAAGGCAGACAAATCAGTTCTAGGAACGGC 5 0.125 No Hit AAGTTGCTAAAGAAGATGACGCTGATGATATAGAGAGAGAATTATACATC 5 0.125 No Hit AGAAGCTGCTGCACTGTTGATGCGTGATCGTGCTGCTTATGAACAAAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0125 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.125 0.0 0.0 0.0 0.0 60-61 0.16249999999999998 0.0 0.0 0.0 0.0 62-63 0.2 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.275 0.0 0.0 0.0 0.0 68-69 0.3 0.0 0.0 0.0 0.0 70-71 0.3375 0.0 0.0 0.0 0.0 72-73 0.4 0.0 0.0 0.0 0.0 74-75 0.4875 0.0 0.0 0.0 0.0 76-77 0.6 0.0 0.0 0.0 0.0 78-79 0.8375 0.0 0.0 0.0 0.0 80-81 0.925 0.0 0.0 0.0 0.0 82-83 1.0 0.0 0.0 0.0 0.0 84-85 1.0625 0.0 0.0 0.0 0.0 86-87 1.2999999999999998 0.0 0.0 0.0 0.0 88-89 1.6875 0.0 0.0 0.0 0.0 90-91 2.05 0.0 0.0 0.0 0.0 92-93 2.4749999999999996 0.0 0.0 0.0 0.0 94-95 2.7625 0.0 0.0 0.0 0.0 96-97 3.1875 0.0 0.0 0.0 0.0 98-99 3.5375 0.0 0.0 0.0 0.0 100-101 3.8125 0.0 0.0 0.0 0.0 102-103 4.4 0.0 0.0 0.0 0.0 104-105 5.225 0.0 0.0 0.0 0.0 106-107 6.025 0.0 0.0 0.0 0.0 108-109 6.6 0.0 0.0 0.0 0.0 110-111 7.175000000000001 0.0 0.0 0.0 0.0 112-113 7.8125 0.0 0.0 0.0 0.0 114-115 8.3625 0.0 0.0 0.0 0.0 116-117 9.1125 0.0 0.0 0.0 0.0 118-119 9.675 0.0 0.0 0.0 0.0 120-121 10.3875 0.0 0.0 0.0 0.0 122-123 10.9625 0.0 0.0 0.0 0.0 124-125 11.775 0.0 0.0 0.0 0.0 126-127 12.7875 0.0 0.0 0.0 0.0 128-129 13.5125 0.0 0.0 0.0 0.0 130-131 14.425 0.0 0.0 0.0 0.0 132-133 15.2375 0.0 0.0 0.0 0.0 134-135 16.2375 0.0 0.0 0.0 0.0 136-137 17.25 0.0 0.0 0.0 0.0 138-139 18.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCTTCC 10 0.006830828 145.0 9 GTATTTG 10 0.006830828 145.0 3 ATTTGCC 10 0.006830828 145.0 5 >>END_MODULE Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490436 spots for SRR13165368.sra Written 1490436 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra Read 1490435 spots for SRR13165368.sra Written 1490435 spots for SRR13165368.sra SRR ids: ['SRR13165368.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_sesobpax SRR13165368.sra spots: 29808701 blocks: [[1, 1490435], [1490436, 2980870], [2980871, 4471305], [4471306, 5961740], [5961741, 7452175], [7452176, 8942610], [8942611, 10433045], [10433046, 11923480], [11923481, 13413915], [13413916, 14904350], [14904351, 16394785], [16394786, 17885220], [17885221, 19375655], [19375656, 20866090], [20866091, 22356525], [22356526, 23846960], [23846961, 25337395], [25337396, 26827830], [26827831, 28318265], [28318266, 29808701]] SRR13165368 file size 10108600 SRR13165368 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165368 SRR13165368_1.fastq SRR13165368_2.fastq Input file: SRR13165368_1.fastq Paired file: SRR13165368_2.fastq trimmed: SRR13165368-trimmed-pair1.fastq, SRR13165368-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:18:32 2024 >> started Sat Dec 7 16:19:05 2024 >> done (33.134s) 29808701 read pairs processed; of these: 1206 ( 0.00%) short read pairs filtered out after trimming by size control 448503 ( 1.50%) empty read pairs filtered out after trimming by size control 29358992 (98.49%) read pairs available; of these: 6580369 (22.41%) trimmed read pairs available after processing 22778623 (77.59%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 74 0.00% 19 86 0.00% 20 74 0.00% 21 87 0.00% 22 106 0.00% 23 141 0.00% 24 173 0.00% 25 139 0.00% 26 179 0.00% 27 207 0.00% 28 246 0.00% 29 262 0.00% 30 298 0.00% 31 318 0.00% 32 319 0.00% 33 279 0.00% 34 345 0.00% 35 356 0.00% 36 359 0.00% 37 356 0.00% 38 379 0.00% 39 398 0.00% 40 501 0.00% 41 599 0.00% 42 596 0.00% 43 655 0.00% 44 577 0.00% 45 604 0.00% 46 802 0.00% 47 823 0.00% 48 953 0.00% 49 1046 0.00% 50 1204 0.00% 51 1387 0.00% 52 1558 0.01% 53 1675 0.01% 54 1897 0.01% 55 1942 0.01% 56 2070 0.01% 57 2282 0.01% 58 2616 0.01% 59 2999 0.01% 60 3379 0.01% 61 3765 0.01% 62 4225 0.01% 63 4765 0.02% 64 5095 0.02% 65 5416 0.02% 66 5822 0.02% 67 6647 0.02% 68 7345 0.03% 69 8056 0.03% 70 9086 0.03% 71 10070 0.03% 72 11600 0.04% 73 12670 0.04% 74 14144 0.05% 75 15439 0.05% 76 16905 0.06% 77 18445 0.06% 78 19547 0.07% 79 21399 0.07% 80 23345 0.08% 81 25408 0.09% 82 28052 0.10% 83 30608 0.10% 84 34162 0.12% 85 36451 0.12% 86 39279 0.13% 87 40325 0.14% 88 42962 0.15% 89 44367 0.15% 90 47432 0.16% 91 49063 0.17% 92 51985 0.18% 93 55503 0.19% 94 59413 0.20% 95 61918 0.21% 96 65063 0.22% 97 67370 0.23% 98 69093 0.24% 99 71415 0.24% 100 72844 0.25% 101 74595 0.25% 102 76966 0.26% 103 77495 0.26% 104 80990 0.28% 105 81828 0.28% 106 85044 0.29% 107 85002 0.29% 108 87382 0.30% 109 90020 0.31% 110 89906 0.31% 111 91959 0.31% 112 93084 0.32% 113 93964 0.32% 114 95624 0.33% 115 98255 0.33% 116 100572 0.34% 117 101567 0.35% 118 102267 0.35% 119 103658 0.35% 120 104767 0.36% 121 106454 0.36% 122 105251 0.36% 123 107681 0.37% 124 108559 0.37% 125 110907 0.38% 126 111827 0.38% 127 112218 0.38% 128 111858 0.38% 129 115203 0.39% 130 114142 0.39% 131 114927 0.39% 132 115412 0.39% 133 115573 0.39% 134 115915 0.39% 135 117096 0.40% 136 117523 0.40% 137 117223 0.40% 138 116931 0.40% 139 119291 0.41% 140 120987 0.41% 141 121091 0.41% 142 120751 0.41% 143 120679 0.41% 144 122259 0.42% 145 123637 0.42% 146 123691 0.42% 147 126605 0.43% 148 124973 0.43% 149 128319 0.44% 150 126596 0.43% 151 22778623 77.59% 29358992 reads passed initial QC criterion=sequence-density sequence-density=0.84 sequence-density-rank=1 fanout-score=2.39 fanout-score-rank=23 prefix-density=0.84 prefix-fanout=2.4 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=50.10 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=7.9 sequence=TTGCATCAATGATAATCCCAAAGTAGGAAGTGTGTACGTAGTAGCAAGTGTTATACATACATAAAATTAAGCGATGCGTTTCGATCAAGTACTTGGCATCATCGATCACATACGTACATTTCACAGCAGGTAGCTACGTACATTACAGGCATACGTACATGCAGAGAGATACCCGGCCCTGTGTTGTGTTTGCAATTGCATAGATGAGAATGAGATGACTTGATTCTTAATTAGCCGGCGAGAGCGCTGGTGTTGTAGACGAGGAGGGCGCCGCCGCCGAGGAGGCCGGCCAGGGTGACGGCCCAGATCAGGAGCCCCGTCGTCCCACCGGCGTAGCGGTCGCCAGATTCGGACCATACCTCCGGCGTGTAGATCGGGCTGTAGCCGTCGACGTTGGCGCCGTACTTGTCAACAAACTGGTACACACCCTTTCCCGTGCCCTTCCTGCCGTTGGCGTCGATGTCCACCGTCAAGCCACCTCCGATCCCGA criterion=sequence-density sequence-density=0.45 sequence-density-rank=1 fanout-score=3.04 fanout-score-rank=22 prefix-density=0.53 prefix-fanout=2.6 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.11 sequence-density-rank=14 fanout-score=99.06 fanout-score-rank=1 prefix-density=0.70 prefix-fanout=15.9 sequence=GCCGCCGCCGCCA SRR13165368 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:19:53 Started mapping on | Dec 07 16:19:53 Finished on | Dec 07 16:22:29 Mapping speed, Million of reads per hour | 677.52 Number of input reads | 29358992 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 26915177 Uniquely mapped reads % | 91.68% Average mapped length | 287.24 Number of splices: Total | 25739449 Number of splices: Annotated (sjdb) | 23975570 Number of splices: GT/AG | 25380616 Number of splices: GC/AG | 308568 Number of splices: AT/AC | 12533 Number of splices: Non-canonical | 37732 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.66 Insertion rate per base | 0.01% Insertion average length | 2.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 781677 % of reads mapped to multiple loci | 2.66% Number of reads mapped to too many loci | 178926 % of reads mapped to too many loci | 0.61% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.47% % of reads unmapped: other | 2.58% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1662448 1662448 1662448 N_multimapping 781677 781677 781677 N_noFeature 1162669 26139107 1398486 N_ambiguous 651454 4323 111625 UnstrandedReadsAssigned:25101054 PositiveStrandReadsAssigned:771747 NegativeStrandReadsAssigned:25405066 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR13165368 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165368-trimmed-pair1.fastq SRR13165368-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,358,992 reads, 25,851,438 reads pseudoaligned [quant] estimated average fragment length: 230.919 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,210 rounds 52973 SRR13165368.ke.tsv 35125 SRR13165368.se.tsv 88098 total ==> SRR13165368.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 706.787 0 0 PNS24247 1044 814.081 57.8064 3.83846 PNS24249 1928 1698.08 178.169 5.67182 PNS24246 1044 814.081 57.8064 3.83846 PNS24248 1044 814.081 57.8064 3.83846 PNS24244 1471 1241.08 176.412 7.68379 PNS24243 293 114.854 0 0 KQK14069 1603 1373.08 454.639 17.8986 KQK14071 474 260.193 13.2938 2.76186 ==> SRR13165368.se.tsv <== BRADI_1g14170v3 481 BRADI_1g53295v3 175 BRADI_1g59795v3 536 BRADI_1g07683v3 0 BRADI_1g00485v3 4 BRADI_1g20270v3 462 BRADI_1g74790v3 927 BRADI_1g09890v3 0 BRADI_1g77505v3 396 BRADI_1g48960v3 1 SRR13165368 completed mapping pipeline successfully