Starting /dee2/code/volunteer_pipeline.sh SRR13165370 current disk space = 1541762691072 free memory = 1599100156 SRR13165370 SRAfilesize 78c26bbef72aa297dc56ceb85d75e015 SRR13165370.sra SRR13165370.sra file validated SRR13165370 is paired end SRR13165370 is conventional basespace SRR13165370 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165370_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5425 37.0 37.0 37.0 37.0 37.0 2 36.32275 37.0 37.0 37.0 37.0 37.0 3 36.524 37.0 37.0 37.0 37.0 37.0 4 36.568 37.0 37.0 37.0 37.0 37.0 5 36.569 37.0 37.0 37.0 37.0 37.0 6 36.5375 37.0 37.0 37.0 37.0 37.0 7 36.49 37.0 37.0 37.0 37.0 37.0 8 36.53 37.0 37.0 37.0 37.0 37.0 9 36.563 37.0 37.0 37.0 37.0 37.0 10-14 36.5155 37.0 37.0 37.0 37.0 37.0 15-19 36.4985 37.0 37.0 37.0 37.0 37.0 20-24 36.504200000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.458800000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.460100000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.412400000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.3913 37.0 37.0 37.0 37.0 37.0 45-49 36.3158 37.0 37.0 37.0 37.0 37.0 50-54 36.3361 37.0 37.0 37.0 37.0 37.0 55-59 36.242999999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.3116 37.0 37.0 37.0 37.0 37.0 65-69 36.268899999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.294200000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.2702 37.0 37.0 37.0 37.0 37.0 80-84 36.2096 37.0 37.0 37.0 37.0 37.0 85-89 36.259699999999995 37.0 37.0 37.0 37.0 37.0 90-94 36.1668 37.0 37.0 37.0 37.0 37.0 95-99 36.159299999999995 37.0 37.0 37.0 37.0 37.0 100-104 36.147200000000005 37.0 37.0 37.0 37.0 37.0 105-109 36.166399999999996 37.0 37.0 37.0 37.0 37.0 110-114 36.03070000000001 37.0 37.0 37.0 37.0 37.0 115-119 36.0801 37.0 37.0 37.0 37.0 37.0 120-124 36.0069 37.0 37.0 37.0 37.0 37.0 125-129 36.0238 37.0 37.0 37.0 37.0 37.0 130-134 35.9323 37.0 37.0 37.0 37.0 37.0 135-139 35.8948 37.0 37.0 37.0 37.0 37.0 140-144 35.735200000000006 37.0 37.0 37.0 37.0 37.0 145-149 35.4885 37.0 37.0 37.0 37.0 37.0 150-151 35.33775 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 3.0 25 3.0 26 7.0 27 10.0 28 15.0 29 13.0 30 28.0 31 40.0 32 49.0 33 81.0 34 171.0 35 317.0 36 2831.0 37 431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.9 9.925 5.7250000000000005 34.449999999999996 2 21.595184349134687 10.835214446952596 34.13594181088538 33.43365939302734 3 21.2 14.424999999999999 26.125 38.25 4 27.750000000000004 19.625 23.7 28.925 5 28.775000000000002 26.875 22.5 21.85 6 24.075 30.049999999999997 22.075 23.799999999999997 7 19.25 25.75 36.725 18.275 8 19.2 22.0 32.6 26.200000000000003 9 21.325 21.425 31.85 25.4 10-14 23.26 25.66 25.835 25.245 15-19 23.505000000000003 24.15 26.13 26.215 20-24 23.765 24.975 25.130000000000003 26.13 25-29 23.794999999999998 24.044999999999998 25.319999999999997 26.840000000000003 30-34 23.755000000000003 24.375 25.94 25.929999999999996 35-39 23.535 24.6 25.845000000000002 26.02 40-44 23.21 23.94 26.045 26.805 45-49 22.73 25.35 25.505 26.415 50-54 23.825 24.8 25.46 25.915 55-59 23.84 25.09 24.685000000000002 26.384999999999998 60-64 23.7 24.565 25.145 26.590000000000003 65-69 23.53 25.335 25.369999999999997 25.765 70-74 25.095 24.060000000000002 24.775 26.07 75-79 23.990000000000002 24.665 25.465 25.88 80-84 24.055 24.435000000000002 25.1 26.41 85-89 25.2 25.465 23.835 25.5 90-94 24.365000000000002 24.474999999999998 25.5 25.66 95-99 24.505 23.905 25.11 26.479999999999997 100-104 24.044999999999998 25.27 24.905 25.779999999999998 105-109 24.415 25.09 24.11 26.384999999999998 110-114 25.135 24.959999999999997 24.125 25.779999999999998 115-119 24.765 24.975 23.845 26.415 120-124 24.37 25.264999999999997 23.369999999999997 26.995 125-129 24.990000000000002 24.545 23.165 27.3 130-134 24.075 24.775 23.580000000000002 27.57 135-139 24.54 24.315 24.44 26.705000000000002 140-144 24.39 25.009999999999998 24.044999999999998 26.555 145-149 24.2 24.610000000000003 24.075 27.115000000000002 150-151 24.3875 24.15 23.9 27.5625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 0.5 26 0.5 27 3.5 28 4.0 29 4.5 30 14.0 31 19.0 32 17.5 33 12.5 34 19.0 35 30.0 36 46.5 37 70.5 38 75.0 39 85.0 40 105.5 41 130.0 42 149.5 43 181.0 44 179.0 45 176.5 46 186.0 47 170.0 48 174.5 49 166.5 50 154.5 51 136.0 52 110.5 53 132.5 54 153.0 55 129.0 56 114.0 57 97.5 58 83.0 59 88.5 60 88.0 61 76.5 62 69.5 63 69.0 64 57.5 65 47.5 66 56.5 67 52.5 68 39.0 69 40.0 70 38.5 71 27.5 72 23.5 73 19.5 74 12.5 75 15.0 76 18.5 77 13.5 78 7.5 79 3.0 80 1.0 81 2.0 82 1.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.325 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.55 #Duplication Level Percentage of deduplicated Percentage of total 1 69.91117690599555 47.225 2 18.985936343449296 25.650000000000002 3 6.994818652849741 14.174999999999999 4 2.7387120651369354 7.3999999999999995 5 0.7401924500370096 2.5 6 0.1850481125092524 0.75 7 0.1850481125092524 0.8750000000000001 8 0.22205773501110287 1.2 9 0.03700962250185048 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGGTTCAGATCGAATATATAAATACTGTCGCTATCCCTCAAGTTACAAT 9 0.22499999999999998 No Hit CTAGATAATTCGAGCTCGTCCTCTGGAATAAGCTCCAACGCTTCGCATAT 8 0.2 No Hit GGTGTTGGAAGATTTAACTGCACTAGAAGAGTAACCATTTGTACAATGCT 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATCGCGTAT 8 0.2 TruSeq Adapter, Index 18 (97% over 38bp) CTCCAGATTAGCCTTAAACCCCGTCCCTTCCTTAATCGCGTCCCTGATGC 8 0.2 No Hit GGGGTGGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTT 8 0.2 No Hit AGCTGATTACTTTTGGGACTTCAGTGCATCTGGCTGTATAGGCATTGATC 8 0.2 No Hit CTCTCCTTCCTCCGGCTTAACACCGGCGGTCTGTTCAGGGTTCCAAACTC 7 0.17500000000000002 No Hit GCCCACGTAACAAATGATGTTTTGCCCCTTTCTGAATCTGGGCACATGGT 7 0.17500000000000002 No Hit TACACACACACTGAGCTGAGCTGAACAAAACATACAAGGCAACAAACCAC 7 0.17500000000000002 No Hit CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT 7 0.17500000000000002 No Hit GGAGCTTTGGCCTGCCTATCAAATCCCATTGCTTCTAGGTATCTCTGGAA 7 0.17500000000000002 No Hit GGGGCGCTGAGGTGCGGGAGGTGGCCCTCCGTGGGCAGAGGCTCGATGGT 6 0.15 No Hit CTTGGGGAAGATCAGCCTGTTATCCCTAGAGTAACTTTTATCCGTTGAGC 6 0.15 No Hit CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGA 6 0.15 No Hit GTCAGTAATGGGGAAGCAAAATCGGTAAACGGGTATGCACCTCTAAAGGC 6 0.15 No Hit GGGCTATCGCTTCCTCTGCTACTTCCTCTAGTTTCCAAACGCTCCATCAT 6 0.15 No Hit CCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCA 5 0.125 No Hit GCTGCATAAGGATGTTGTGCTCTGCCTGGAATACAATCATAAAGTTGAAA 5 0.125 No Hit GGTTAGCGTAATTAAGCTGGGGTTACTAGTAGTGTAGTACGGGTAGAGGC 5 0.125 No Hit TCTTGCATGGTTGACATGTTCTCCACTGGTTTGTGTTCACTGAAGAACAT 5 0.125 No Hit CTGGATAGCTAGTTTTTAGCAGATGCTTGATAAAAGTTGTTTTCCCCGTA 5 0.125 No Hit GCTCACGGCGCCCAGGGCTTTCGACTCCGACTCCGGGCGCCGGTCCATCA 5 0.125 No Hit CCTGGCTCGAGCGAGAAAAACAGGAGGCAATATTTCATCAACAACAGCAC 5 0.125 No Hit TTTCAGAGCTTAAATCATCTGTGGCATCATGATGGCGTCTTTTATGCTTC 5 0.125 No Hit GCCAGCTCCTATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGGAT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGATCATCTCGTAT 5 0.125 TruSeq Adapter, Index 18 (97% over 38bp) GCATTCAATTTTGTTATTGGAAAAAACATTAGGAACAATTGAGTATAAAA 5 0.125 No Hit CCCAAACATAGCTGTTCTTCTTCCTCCACGAGCTTTTCCTCTGCAAAAAG 5 0.125 No Hit GCTTGTATTGCTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCA 5 0.125 No Hit GTGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTC 5 0.125 No Hit CTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCT 5 0.125 No Hit CAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCAC 5 0.125 No Hit GTCCTGACGACGCCGCACATGACGGCGTCCCTGGGCACTTTGAGGTCACG 5 0.125 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 5 0.125 No Hit GCCGTTTCCCTTCTTCACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGC 5 0.125 No Hit CCAGCTCGGCAAACTTCACGGCAATGTGGGAGGTGTGGCAGTCCAGCACT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.07500000000000001 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.48750000000000004 0.0 0.0 0.0 0.0 82-83 0.6625 0.0 0.0 0.0 0.0 84-85 0.7875000000000001 0.0 0.0 0.0 0.0 86-87 0.9125 0.0 0.0 0.0 0.0 88-89 1.225 0.0 0.0 0.0 0.0 90-91 1.5375 0.0 0.0 0.0 0.0 92-93 2.0125 0.0 0.0 0.0 0.0 94-95 2.45 0.0 0.0 0.0 0.0 96-97 2.9125 0.0 0.0 0.0 0.0 98-99 3.1624999999999996 0.0 0.0 0.0 0.0 100-101 3.6625 0.0 0.0 0.0 0.0 102-103 4.0125 0.0 0.0 0.0 0.0 104-105 4.6375 0.0 0.0 0.0 0.0 106-107 5.375 0.0 0.0 0.0 0.0 108-109 6.1875 0.0 0.0 0.0 0.0 110-111 6.775 0.0 0.0 0.0 0.0 112-113 7.525 0.0 0.0 0.0 0.0 114-115 8.399999999999999 0.0 0.0 0.0 0.0 116-117 9.0875 0.0 0.0 0.0 0.0 118-119 9.899999999999999 0.0 0.0 0.0 0.0 120-121 10.850000000000001 0.0 0.0 0.0 0.0 122-123 11.85 0.0 0.0 0.0 0.0 124-125 12.8625 0.0 0.0 0.0 0.0 126-127 13.8875 0.0 0.0 0.0 0.0 128-129 15.2125 0.0 0.0 0.0 0.0 130-131 16.1875 0.0 0.0 0.0 0.0 132-133 16.737499999999997 0.0 0.0 0.0 0.0 134-135 17.7625 0.0 0.0 0.0 0.0 136-137 18.4 0.0 0.0 0.0 0.0 138-139 19.387500000000003 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13165370 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165370_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.18275 37.0 37.0 37.0 37.0 37.0 2 36.2985 37.0 37.0 37.0 37.0 37.0 3 36.1675 37.0 37.0 37.0 37.0 37.0 4 36.3235 37.0 37.0 37.0 37.0 37.0 5 36.461 37.0 37.0 37.0 37.0 37.0 6 36.341 37.0 37.0 37.0 37.0 37.0 7 36.2685 37.0 37.0 37.0 37.0 37.0 8 36.342 37.0 37.0 37.0 37.0 37.0 9 36.3285 37.0 37.0 37.0 37.0 37.0 10-14 36.3382 37.0 37.0 37.0 37.0 37.0 15-19 36.326499999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.286 37.0 37.0 37.0 37.0 37.0 25-29 36.213100000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.17075 37.0 37.0 37.0 37.0 37.0 35-39 36.159499999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.1382 37.0 37.0 37.0 37.0 37.0 45-49 36.08305 37.0 37.0 37.0 37.0 37.0 50-54 36.11225 37.0 37.0 37.0 37.0 37.0 55-59 36.063399999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.144099999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.0392 37.0 37.0 37.0 37.0 37.0 70-74 35.898 37.0 37.0 37.0 37.0 37.0 75-79 35.96575 37.0 37.0 37.0 37.0 37.0 80-84 35.96155 37.0 37.0 37.0 37.0 37.0 85-89 35.8971 37.0 37.0 37.0 37.0 37.0 90-94 35.98805 37.0 37.0 37.0 37.0 37.0 95-99 36.0187 37.0 37.0 37.0 37.0 37.0 100-104 35.9079 37.0 37.0 37.0 37.0 37.0 105-109 35.90575 37.0 37.0 37.0 37.0 37.0 110-114 35.7952 37.0 37.0 37.0 37.0 37.0 115-119 35.7078 37.0 37.0 37.0 37.0 37.0 120-124 35.6183 37.0 37.0 37.0 37.0 37.0 125-129 35.56935 37.0 37.0 37.0 37.0 37.0 130-134 35.3436 37.0 37.0 37.0 34.6 37.0 135-139 35.27034999999999 37.0 37.0 37.0 34.6 37.0 140-144 35.03920000000001 37.0 37.0 37.0 29.8 37.0 145-149 34.77775 37.0 37.0 37.0 25.0 37.0 150-151 34.45825 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 4.0 15 4.0 16 0.0 17 2.0 18 2.0 19 2.0 20 1.0 21 1.0 22 6.0 23 1.0 24 9.0 25 7.0 26 9.0 27 9.0 28 16.0 29 14.0 30 26.0 31 34.0 32 71.0 33 110.0 34 189.0 35 475.0 36 2731.0 37 272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.04767879548306 20.125470514429107 8.48180677540778 25.345043914680048 2 31.025000000000002 23.25 25.424999999999997 20.3 3 24.474999999999998 25.0 27.975 22.55 4 28.299999999999997 30.7 19.7 21.3 5 30.225 32.225 18.3 19.25 6 23.974999999999998 36.55 17.849999999999998 21.625 7 25.55 20.424999999999997 31.05 22.975 8 24.6 23.849999999999998 22.55 28.999999999999996 9 24.275 22.475 26.35 26.900000000000002 10-14 26.595000000000002 26.27 22.49 24.645 15-19 27.284999999999997 24.834999999999997 23.845 24.035 20-24 26.99929936943249 24.802322089880892 23.796416775097587 24.401961765589032 25-29 27.04056084126189 24.321482223335003 23.09464196294442 25.543314972458685 30-34 26.215147419532464 25.549381789057417 23.52705611453171 24.70841467687841 35-39 25.758182364127713 25.29776799119207 23.55620058052247 25.38784906415774 40-44 26.962354825790953 24.819783740488585 22.932519022827393 25.28534241089307 45-49 26.883227388758197 24.846088392812455 24.26047349717203 24.01021072125732 50-54 26.77776109693239 24.44077465845969 24.04043436921383 24.741029875394087 55-59 27.03054581872809 25.172759138708063 23.795693540310467 24.00100150225338 60-64 26.313682314082676 25.13261935742168 24.597137423681314 23.956560904814335 65-69 27.329130391273893 24.792354648253777 23.70659461623136 24.17192034424097 70-74 27.806710065097644 25.15773660490736 23.01952929394091 24.01602403605408 75-79 27.79974968710889 25.201501877346683 23.294117647058822 23.704630788485606 80-84 27.31548661496122 25.55416562421816 23.45258944208156 23.677758318739052 85-89 28.234528339675546 24.58942519527338 23.277588624073704 23.89845784097737 90-94 27.02066963615435 24.5583304138932 24.383164005805515 24.03783594414694 95-99 27.261809447558043 25.535428342674138 23.383706965572458 23.819055244195354 100-104 28.057085628442664 24.877315973960943 23.16975463194792 23.895843765648472 105-109 27.81032497120825 25.341745531019978 22.773020880276402 24.074908617495367 110-114 28.16534881393254 25.277749974977482 23.0307276548894 23.52617355620058 115-119 29.11576206689365 24.844782695774086 23.257560584818744 22.781894652513518 120-124 28.85327991987982 25.633450175262894 22.493740610916372 23.01952929394091 125-129 29.695088369298556 24.88359285034797 22.97601762379212 22.44530115656136 130-134 30.09917852133841 25.465838509316768 22.625726307353236 21.809256661991586 135-139 30.40016026443632 25.487053638503532 22.992938348274652 21.119847748785496 140-144 31.72355119607647 25.222700430387345 22.470223200880792 20.58352517265539 145-149 31.837531927680672 24.93614463865378 22.992938348274652 20.233385085390896 150-151 32.94913555499875 25.181658732147334 22.012027060886997 19.857178651966926 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.5 8 0.5 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 1.0 19 1.0 20 0.0 21 0.0 22 0.0 23 0.5 24 1.0 25 0.5 26 0.5 27 1.0 28 4.0 29 4.5 30 4.0 31 10.5 32 15.5 33 25.0 34 27.0 35 24.5 36 40.5 37 52.0 38 75.5 39 99.5 40 96.5 41 113.0 42 135.5 43 160.0 44 167.5 45 152.0 46 167.0 47 149.0 48 150.0 49 166.5 50 153.0 51 153.0 52 135.0 53 124.5 54 137.5 55 131.0 56 104.5 57 109.0 58 102.5 59 89.0 60 88.0 61 80.0 62 73.5 63 64.5 64 66.0 65 64.0 66 60.5 67 53.5 68 48.5 69 54.5 70 49.5 71 48.5 72 43.5 73 23.5 74 14.5 75 13.0 76 14.0 77 10.0 78 2.5 79 1.5 80 2.5 81 2.5 82 0.5 83 0.0 84 0.0 85 0.0 86 1.0 87 1.0 88 0.0 89 0.5 90 1.5 91 2.5 92 3.0 93 3.5 94 2.0 95 0.0 96 0.0 97 1.5 98 1.5 99 2.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.09 25-29 0.15 30-34 0.11499999999999999 35-39 0.09 40-44 0.12 45-49 0.105 50-54 0.08499999999999999 55-59 0.15 60-64 0.09 65-69 0.06999999999999999 70-74 0.15 75-79 0.125 80-84 0.075 85-89 0.13999999999999999 90-94 0.095 95-99 0.08 100-104 0.15 105-109 0.145 110-114 0.09 115-119 0.13999999999999999 120-124 0.15 125-129 0.135 130-134 0.18 135-139 0.165 140-144 0.09 145-149 0.165 150-151 0.22499999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.4 #Duplication Level Percentage of deduplicated Percentage of total 1 71.16228070175438 48.675000000000004 2 18.055555555555554 24.7 3 6.761695906432749 13.875000000000002 4 2.814327485380117 7.7 5 0.6944444444444444 2.375 6 0.07309941520467836 0.3 7 0.25584795321637427 1.225 8 0.10964912280701754 0.6 9 0.03654970760233918 0.22499999999999998 >10 0.03654970760233918 0.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 13 0.325 No Hit GAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAA 9 0.22499999999999998 No Hit GCAAAGAATTAGATGCTATACTGATACACCCGAAGCCAGTTAGCTGCTCC 8 0.2 No Hit GACTGAGCAAGTGGATCGGGAGGACCGGATTCCAGTCCTCAAGGCATCAC 8 0.2 No Hit GGTTTACTTAATACGCTCAAAAGTCTGAGTTGTGCTAGGAATAATTAAAC 8 0.2 No Hit GTATCGTCCATTCAGGGTTCTCCCCAGAGTCTGGTCACTACTATGCATAT 7 0.17500000000000002 No Hit GGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGG 7 0.17500000000000002 No Hit GTGGACCTTTTCCGGGCACATGATCCCCGATACGTGAAACCCAGATGGCC 7 0.17500000000000002 No Hit GCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACG 7 0.17500000000000002 No Hit GGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGCAAG 7 0.17500000000000002 No Hit GGTCATTAAAGTCTTAAAGCCTGGTATTGAAGATACTTTGGTTGCCGATC 7 0.17500000000000002 No Hit AGTAGCGGTGCTGTGCTTTGCTGCCGTCTGTATATTATTAAGTAGAAATG 7 0.17500000000000002 No Hit CAATCACCTTTTGCAAACAAATTGGTTCAGCTCAAAATCAGCATAGAAGA 6 0.15 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAA 6 0.15 No Hit GTATGCGCCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTGCG 5 0.125 No Hit GTCAAGGAGAGAGGAAATGGAGATCGATGGAGCTGACATGGAGACTCATG 5 0.125 No Hit ACTATATGTCGAAAAATTGAAGCCTTTGGAAGTTACATACAAATTCAATG 5 0.125 No Hit ACTGGTCTTACCACTGAGGTCAAGTCCGTTGAGATGCACCATGAGGCTCT 5 0.125 No Hit CCTTTAACAAGTTGCATTGACAGCGGTATCAGTTGCAACTACACCTTCTT 5 0.125 No Hit GTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC 5 0.125 No Hit CTGGAAACGGTTGCTAATACCCCGTAGGCTGAGGAGCAAAAGGAGAAATC 5 0.125 No Hit CAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATT 5 0.125 No Hit GAGCGGCTGCGGCAACTGCGACTGCGCTGACAAGACCCAGTGTGTGAAGA 5 0.125 No Hit CCTGAACGCCAACTGGGGCACGCTGTCGTGCAAGAACAAGAAGAGCTTCA 5 0.125 No Hit ATTAAATAAGATGGTGATTGCTAGCAGGTAGTTGTGTTCTACTCCCTATT 5 0.125 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 5 0.125 No Hit GGAGGGACAACGACAGCGAGACCCACCCGTGGCACCTCCACGGGCACGAC 5 0.125 No Hit TGGACCGCCATGATAAGGAAAAAGAGATGGCGTCAATTCTGTTGTCTGCC 5 0.125 No Hit ACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTCGAG 5 0.125 No Hit CAGAGTTCCATACTGATGAAAGCTGCGAGCCATGCCGAACTGCAGATATT 5 0.125 No Hit CACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAG 5 0.125 No Hit AATCAACAACTAGCAAAGAAGAGCAGAGCAGAGCGAGGGGAATAAAGCAG 5 0.125 No Hit GCAAGACCTTCTTCATCCCGATCTCCACGCTGCTGCTCGACCGCGTCGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.07500000000000001 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.48750000000000004 0.0 0.0 0.0 0.0 82-83 0.6625 0.0 0.0 0.0 0.0 84-85 0.7875000000000001 0.0 0.0 0.0 0.0 86-87 0.9125 0.0 0.0 0.0 0.0 88-89 1.2125 0.0 0.0 0.0 0.0 90-91 1.5125 0.0 0.0 0.0 0.0 92-93 1.9875 0.0 0.0 0.0 0.0 94-95 2.3875 0.0 0.0 0.0 0.0 96-97 2.85 0.0 0.0 0.0 0.0 98-99 3.1125 0.0 0.0 0.0 0.0 100-101 3.625 0.0 0.0 0.0 0.0 102-103 4.025 0.0 0.0 0.0 0.0 104-105 4.6875 0.0 0.0 0.0 0.0 106-107 5.425000000000001 0.0 0.0 0.0 0.0 108-109 6.2375 0.0 0.0 0.0 0.0 110-111 6.8375 0.0 0.0 0.0 0.0 112-113 7.575 0.0 0.0 0.0 0.0 114-115 8.45 0.0 0.0 0.0 0.0 116-117 9.1375 0.0 0.0 0.0 0.0 118-119 9.9875 0.0 0.0 0.0 0.0 120-121 10.95 0.0 0.0 0.0 0.0 122-123 11.95 0.0 0.0 0.0 0.0 124-125 12.9625 0.0 0.0 0.0 0.0 126-127 14.0 0.0 0.0 0.0 0.0 128-129 15.3125 0.0 0.0 0.0 0.0 130-131 16.3125 0.0 0.0 0.0 0.0 132-133 16.862499999999997 0.0 0.0 0.0 0.0 134-135 17.9 0.0 0.0 0.0 0.0 136-137 18.575 0.0 0.0 0.0 0.0 138-139 19.5875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111437 spots for SRR13165370.sra Written 1111437 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra Read 1111428 spots for SRR13165370.sra Written 1111428 spots for SRR13165370.sra SRR ids: ['SRR13165370.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uryjj78j SRR13165370.sra spots: 22228569 blocks: [[1, 1111428], [1111429, 2222856], [2222857, 3334284], [3334285, 4445712], [4445713, 5557140], [5557141, 6668568], [6668569, 7779996], [7779997, 8891424], [8891425, 10002852], [10002853, 11114280], [11114281, 12225708], [12225709, 13337136], [13337137, 14448564], [14448565, 15559992], [15559993, 16671420], [16671421, 17782848], [17782849, 18894276], [18894277, 20005704], [20005705, 21117132], [21117133, 22228569]] SRR13165370 file size 7532539 SRR13165370 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165370 SRR13165370_1.fastq SRR13165370_2.fastq Input file: SRR13165370_1.fastq Paired file: SRR13165370_2.fastq trimmed: SRR13165370-trimmed-pair1.fastq, SRR13165370-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:23:21 2024 >> started Sat Dec 7 16:23:47 2024 >> done (26.016s) 22228569 read pairs processed; of these: 462 ( 0.00%) short read pairs filtered out after trimming by size control 24485 ( 0.11%) empty read pairs filtered out after trimming by size control 22203622 (99.89%) read pairs available; of these: 5449870 (24.54%) trimmed read pairs available after processing 16753752 (75.46%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 27 0.00% 19 30 0.00% 20 33 0.00% 21 28 0.00% 22 52 0.00% 23 62 0.00% 24 63 0.00% 25 45 0.00% 26 70 0.00% 27 71 0.00% 28 73 0.00% 29 81 0.00% 30 98 0.00% 31 110 0.00% 32 128 0.00% 33 114 0.00% 34 141 0.00% 35 136 0.00% 36 148 0.00% 37 177 0.00% 38 196 0.00% 39 207 0.00% 40 237 0.00% 41 219 0.00% 42 286 0.00% 43 318 0.00% 44 304 0.00% 45 330 0.00% 46 333 0.00% 47 384 0.00% 48 504 0.00% 49 563 0.00% 50 694 0.00% 51 727 0.00% 52 862 0.00% 53 896 0.00% 54 969 0.00% 55 1094 0.00% 56 1194 0.01% 57 1363 0.01% 58 1548 0.01% 59 1809 0.01% 60 2080 0.01% 61 2424 0.01% 62 2666 0.01% 63 2847 0.01% 64 3246 0.01% 65 3548 0.02% 66 4003 0.02% 67 4458 0.02% 68 4936 0.02% 69 5461 0.02% 70 6340 0.03% 71 6709 0.03% 72 7985 0.04% 73 9113 0.04% 74 9962 0.04% 75 11047 0.05% 76 12345 0.06% 77 12882 0.06% 78 14469 0.07% 79 16037 0.07% 80 17358 0.08% 81 18950 0.09% 82 20871 0.09% 83 23587 0.11% 84 25982 0.12% 85 27969 0.13% 86 30256 0.14% 87 32342 0.15% 88 33936 0.15% 89 35656 0.16% 90 38017 0.17% 91 40106 0.18% 92 41857 0.19% 93 45260 0.20% 94 47391 0.21% 95 50258 0.23% 96 53128 0.24% 97 55218 0.25% 98 57169 0.26% 99 59602 0.27% 100 60813 0.27% 101 62441 0.28% 102 63462 0.29% 103 65729 0.30% 104 68126 0.31% 105 68427 0.31% 106 71790 0.32% 107 73169 0.33% 108 74780 0.34% 109 77191 0.35% 110 76430 0.34% 111 77144 0.35% 112 79644 0.36% 113 79007 0.36% 114 81678 0.37% 115 84572 0.38% 116 85745 0.39% 117 86273 0.39% 118 87907 0.40% 119 87351 0.39% 120 90732 0.41% 121 89906 0.40% 122 90323 0.41% 123 92485 0.42% 124 93036 0.42% 125 94359 0.42% 126 94331 0.42% 127 94928 0.43% 128 95497 0.43% 129 96172 0.43% 130 96938 0.44% 131 97137 0.44% 132 96982 0.44% 133 97684 0.44% 134 96386 0.43% 135 98089 0.44% 136 99073 0.45% 137 97452 0.44% 138 98001 0.44% 139 99227 0.45% 140 100480 0.45% 141 99646 0.45% 142 101687 0.46% 143 99944 0.45% 144 100104 0.45% 145 101149 0.46% 146 100986 0.45% 147 102868 0.46% 148 102438 0.46% 149 103797 0.47% 150 102159 0.46% 151 16753752 75.46% 22203622 reads passed initial QC criterion=sequence-density sequence-density=0.78 sequence-density-rank=1 fanout-score=2.25 fanout-score-rank=19 prefix-density=0.78 prefix-fanout=2.2 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.25 sequence-density-rank=14 fanout-score=14.50 fanout-score-rank=1 prefix-density=1.07 prefix-fanout=3.4 sequence=CCGCACTTGCACTTGCCGTCGTTCTCCGCCGCGGACTCCTGCACCTCGAAGTGGCTCTTCTCGGTGTCAACCATGACGATGCCGTAGCCGTTTCCCTTCTTCACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGCCGCAGCCGCTCGACATGGTGGCCTTAACTTGCTGGGGAGATCGAGTACACGAATCAGCTGTGTTTTGCCTGTGTGTGAT criterion=sequence-density sequence-density=0.78 sequence-density-rank=1 fanout-score=2.34 fanout-score-rank=29 prefix-density=0.82 prefix-fanout=2.2 sequence=GGTGGTGCATGGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=37 fanout-score=50.98 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=3.1 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGATGTCTGCTACCTTCTCGTCCACCGTCGGAGCTCCGGCTTCTACGCCAACCAGCTTCCTTGGGAAGAAGCTCAAGAAGCAGGTGACCTCGGCCGTGAACTACCATGGCAAGAGCACCAAGGCCAACAGATTCACAGTCATGGCCAAGGAGGTGGACGAGTCAAAGCAGACTGACCAGGACAGGTGGAAGGGCCTCGCCTACGATATCTCCGACGACCAGCAGGACATCACCAGGGGGAAGGGTATCGTCGACTCGCTCTTCCAGGCGCCCATGGGCGACGGTACCCACGTGGCCGTCCTCAGCTCCCAAGAGTACATCAGCCAGGGCCTAAGGAAGTACGACTTCGACAACA SRR13165370 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:24:39 Started mapping on | Dec 07 16:24:39 Finished on | Dec 07 16:27:01 Mapping speed, Million of reads per hour | 562.91 Number of input reads | 22203622 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 19492631 Uniquely mapped reads % | 87.79% Average mapped length | 286.29 Number of splices: Total | 18126253 Number of splices: Annotated (sjdb) | 16905470 Number of splices: GT/AG | 17866938 Number of splices: GC/AG | 218426 Number of splices: AT/AC | 8191 Number of splices: Non-canonical | 32698 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.55 Insertion rate per base | 0.01% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 991476 % of reads mapped to multiple loci | 4.47% Number of reads mapped to too many loci | 225897 % of reads mapped to too many loci | 1.02% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.56% % of reads unmapped: other | 4.16% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1719766 1719766 1719766 N_multimapping 991476 991476 991476 N_noFeature 1252302 18921542 1441159 N_ambiguous 460869 3272 79233 UnstrandedReadsAssigned:17779460 PositiveStrandReadsAssigned:567817 NegativeStrandReadsAssigned:17972239 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=141 echo kmer=137 SRR13165370 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165370-trimmed-pair1.fastq SRR13165370-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,203,622 reads, 18,438,233 reads pseudoaligned [quant] estimated average fragment length: 227.3 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,209 rounds 52973 SRR13165370.ke.tsv 35125 SRR13165370.se.tsv 88098 total ==> SRR13165370.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 710.055 0 0 PNS24247 1044 817.7 51.2194 4.63579 PNS24249 1928 1701.7 173.2 7.53263 PNS24246 1044 817.7 51.2194 4.63579 PNS24248 1044 817.7 51.2194 4.63579 PNS24244 1471 1244.7 90.1422 5.35978 PNS24243 293 117.781 0 0 KQK14069 1603 1376.7 713.125 38.3362 KQK14071 474 264.992 7.36099 2.05583 ==> SRR13165370.se.tsv <== BRADI_1g14170v3 750 BRADI_1g53295v3 102 BRADI_1g59795v3 678 BRADI_1g07683v3 0 BRADI_1g00485v3 1 BRADI_1g20270v3 457 BRADI_1g74790v3 326 BRADI_1g09890v3 0 BRADI_1g77505v3 300 BRADI_1g48960v3 0 SRR13165370 completed mapping pipeline successfully