Starting /dee2/code/volunteer_pipeline.sh SRR13165372 current disk space = 1541752573952 free memory = 1602324080 SRR13165372 SRAfilesize e79405e22109e618ae939c8b268fa4bc SRR13165372.sra SRR13165372.sra file validated SRR13165372 is paired end SRR13165372 is conventional basespace SRR13165372 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165372_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.551 37.0 37.0 37.0 37.0 37.0 2 36.15375 37.0 37.0 37.0 37.0 37.0 3 36.4955 37.0 37.0 37.0 37.0 37.0 4 36.581 37.0 37.0 37.0 37.0 37.0 5 36.513 37.0 37.0 37.0 37.0 37.0 6 36.6365 37.0 37.0 37.0 37.0 37.0 7 36.3675 37.0 37.0 37.0 37.0 37.0 8 36.523 37.0 37.0 37.0 37.0 37.0 9 36.4245 37.0 37.0 37.0 37.0 37.0 10-14 36.535700000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.5165 37.0 37.0 37.0 37.0 37.0 20-24 36.504000000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.491 37.0 37.0 37.0 37.0 37.0 30-34 36.3977 37.0 37.0 37.0 37.0 37.0 35-39 36.387299999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.3984 37.0 37.0 37.0 37.0 37.0 45-49 36.3724 37.0 37.0 37.0 37.0 37.0 50-54 36.361399999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3131 37.0 37.0 37.0 37.0 37.0 60-64 36.2808 37.0 37.0 37.0 37.0 37.0 65-69 36.2525 37.0 37.0 37.0 37.0 37.0 70-74 36.2822 37.0 37.0 37.0 37.0 37.0 75-79 36.267900000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.175200000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.2188 37.0 37.0 37.0 37.0 37.0 90-94 36.10510000000001 37.0 37.0 37.0 37.0 37.0 95-99 36.089999999999996 37.0 37.0 37.0 37.0 37.0 100-104 36.1216 37.0 37.0 37.0 37.0 37.0 105-109 36.1065 37.0 37.0 37.0 37.0 37.0 110-114 36.0001 37.0 37.0 37.0 37.0 37.0 115-119 35.987 37.0 37.0 37.0 37.0 37.0 120-124 35.896 37.0 37.0 37.0 37.0 37.0 125-129 35.761 37.0 37.0 37.0 37.0 37.0 130-134 35.7397 37.0 37.0 37.0 37.0 37.0 135-139 35.6526 37.0 37.0 37.0 37.0 37.0 140-144 35.45 37.0 37.0 37.0 37.0 37.0 145-149 35.0824 37.0 37.0 37.0 27.4 37.0 150-151 34.90725 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 1.0 24 1.0 25 5.0 26 5.0 27 13.0 28 17.0 29 21.0 30 33.0 31 35.0 32 50.0 33 113.0 34 158.0 35 381.0 36 2754.0 37 412.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.15 10.525 4.725 28.599999999999998 2 25.70997738125157 10.630811761749182 31.741643629052525 31.91756722794672 3 21.775 15.725 26.5 36.0 4 28.7 21.325 22.175 27.800000000000004 5 28.95 27.400000000000002 22.075 21.575 6 25.474999999999998 29.375 21.7 23.45 7 21.75 23.225 37.724999999999994 17.299999999999997 8 21.15 23.225 29.349999999999998 26.275 9 20.575 22.45 31.75 25.224999999999998 10-14 24.305 25.97 24.985 24.740000000000002 15-19 23.565 25.605 25.11 25.72 20-24 24.73 24.45 25.540000000000003 25.28 25-29 23.645 24.87 25.685000000000002 25.8 30-34 24.795 24.93 24.23 26.045 35-39 24.62 24.62 25.169999999999998 25.590000000000003 40-44 24.85 24.285 25.14 25.724999999999998 45-49 23.7 25.305 24.2 26.795 50-54 23.955000000000002 24.455 25.595000000000002 25.995 55-59 24.305 25.215 25.374999999999996 25.105 60-64 24.055 25.080000000000002 24.77 26.095000000000002 65-69 23.915 24.925 24.575 26.584999999999997 70-74 25.15 24.45 24.125 26.275 75-79 24.72 25.1 24.54 25.64 80-84 25.4 24.235 24.745 25.619999999999997 85-89 25.495 24.224999999999998 25.025 25.255 90-94 25.095 24.825 24.325 25.755 95-99 24.695 25.52 24.310000000000002 25.474999999999998 100-104 24.945 25.52 24.205 25.330000000000002 105-109 24.985 25.09 23.825 26.1 110-114 25.679999999999996 25.44 23.11 25.77 115-119 25.490000000000002 24.59 23.775 26.145000000000003 120-124 24.55 25.569999999999997 23.31 26.57 125-129 24.740000000000002 25.374999999999996 23.89 25.995 130-134 25.430000000000003 24.845 23.315 26.41 135-139 25.595000000000002 24.834999999999997 22.830000000000002 26.740000000000002 140-144 25.080000000000002 24.709999999999997 24.05 26.16 145-149 25.759999999999998 24.635 23.41 26.195 150-151 25.224999999999998 24.775 23.7375 26.2625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 1.0 24 0.0 25 0.0 26 1.5 27 2.0 28 2.5 29 3.0 30 6.5 31 14.0 32 19.0 33 16.5 34 26.5 35 36.5 36 35.5 37 49.5 38 71.0 39 92.5 40 113.0 41 127.0 42 153.0 43 180.0 44 192.5 45 181.0 46 188.0 47 198.5 48 169.5 49 157.0 50 145.5 51 133.5 52 140.5 53 130.5 54 116.5 55 99.0 56 80.5 57 77.0 58 75.0 59 85.5 60 87.5 61 88.5 62 78.5 63 66.0 64 68.5 65 66.0 66 53.5 67 41.5 68 47.0 69 53.5 70 57.0 71 44.5 72 30.0 73 26.5 74 18.5 75 13.5 76 14.5 77 13.0 78 6.5 79 3.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.0 #Duplication Level Percentage of deduplicated Percentage of total 1 72.46478873239437 51.449999999999996 2 18.767605633802816 26.650000000000002 3 5.915492957746479 12.6 4 1.795774647887324 5.1 5 0.6690140845070423 2.375 6 0.24647887323943662 1.05 7 0.07042253521126761 0.35000000000000003 8 0.035211267605633804 0.2 9 0.035211267605633804 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGAAGAACTTGAGTGCTCCTGATACTGGAAATGATGTTATGTTAGGAAG 9 0.22499999999999998 No Hit GGGAGCGCAGCACTGAGTTCTGGGTCTCCGCTGCCGCGTAGCTCTGCACG 8 0.2 No Hit GGAGGCGATGGAGTAGAGCCTGAGCTTGTGGGGCTTGCCGTTCTTGTCGA 7 0.17500000000000002 No Hit CAAGCTGAAGAAGAGGATGGCGTGGACGATGATGGATATGGCGCTGGTGT 7 0.17500000000000002 No Hit CGCGTATGAGAAGGACACGTGCCACGGGTTCGGCGACTGGTTCATCGCGT 6 0.15 No Hit CCCTTATCGAGCTTATCAAAAATTGTTATCAGAAACTGCTGCAGCATGTC 6 0.15 No Hit CATGGCAGCAGCCAAAAGGACATCTAGGCCACTCCTGAATCCACCATCCA 6 0.15 No Hit GGGGATGAAAAATAGAAAACTCAAAGCAATGTCAGAGTTGACAAAACTGA 6 0.15 No Hit TGGTATTACTGATGCAGTTTCAGCATACTAAAAAATAACAGGACGACTGA 6 0.15 No Hit CAGCACTCACTTAACCCTAGTTCTATGTAGACACATAAAACCCATACAAT 6 0.15 No Hit CGAGGCGTCGATGGTGGAGGAGACGTCGGAGCAGCGGAAGTCGACGGAGA 6 0.15 No Hit GGTGTCACGAACGATCTTCTCATAGTCAACAGTGGCCTTGGTGGTGATCT 5 0.125 No Hit GGCAGGGAAGAGGAGGGTGCCGGATTCCTGCATCTGGTAGAGCCTCTTGA 5 0.125 No Hit CGGCGGTGCATAATCGGCTTGAATTGATAGATCATGAGATGACGAACACC 5 0.125 No Hit GCTGTCTACTGCACTCCTCTGAATGCTTGATGACGCTGTGTCACTATCAT 5 0.125 No Hit AGCGTATCCAACATCCTCGTACCCATCATCCTACGAATATAAGAAATATC 5 0.125 No Hit GTCCCACCAATGTTGTGTTCGAAGCCATTCGAGAATCTCGCTGACCAGAT 5 0.125 No Hit CCTTTTCAAACTTAGTCTCATCAAGGGAATTTCTAAGCTTGAAAACTTCA 5 0.125 No Hit CAGAGAACTCTCCCTCCTCCATGCCCTCACCCACATACCAGTGGACAAAG 5 0.125 No Hit CCTCACCCACAGGACCTGCGCTGCATGACGTGGGAGGGTCCTTCTGCAGG 5 0.125 No Hit CCGGGAAGTGGGAGGAGGCGGCTGCTCTGGTGAGAGAGCCCAGGCACAGG 5 0.125 No Hit GTATTCAGGTGCGCTTTAATCCTTGTAAGGATCATCTCTCTCTGCTGATC 5 0.125 No Hit GTTGGACAGAACTCTGCTGTGGCGCTTGAGAACTAGTGTACACGAACTTT 5 0.125 No Hit GCACTTTCAAGCCAAAACAATCAAACAAGACACTGGAATAAGATCAATCT 5 0.125 No Hit CGGAAGTGTAGTACATGATCAGAACACTCCGAGAAAGAGACATCATCCCG 5 0.125 No Hit TCCAGATTCAATACAACACCTAACATGGAAGATCGCAGCAATTTCACATC 5 0.125 No Hit GTTCCAATAACCTTCTCCAGACGCTGGCCGCATGTAGTCCCGACAGCATC 5 0.125 No Hit CTCTTGACTTGTCAACTGAAGTATCTATTTCTGTTGCTTTCGAATTATCA 5 0.125 No Hit GGGAGTTTGGCTTGCGCGGGGGCTTTGTGCTGTGGCCTGTGGCCGGAGCG 5 0.125 No Hit GCTCCGGATAGTACCTTCAAATAGTACGGGCTCTTGAGGGATGATACCGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.1625 0.0 0.0 0.0 0.0 60-61 0.2375 0.0 0.0 0.0 0.0 62-63 0.25 0.0 0.0 0.0 0.0 64-65 0.3125 0.0 0.0 0.0 0.0 66-67 0.38749999999999996 0.0 0.0 0.0 0.0 68-69 0.4625 0.0 0.0 0.0 0.0 70-71 0.5875 0.0 0.0 0.0 0.0 72-73 0.7 0.0 0.0 0.0 0.0 74-75 0.8 0.0 0.0 0.0 0.0 76-77 0.9125000000000001 0.0 0.0 0.0 0.0 78-79 1.0875 0.0 0.0 0.0 0.0 80-81 1.2000000000000002 0.0 0.0 0.0 0.0 82-83 1.4875 0.0 0.0 0.0 0.0 84-85 1.7000000000000002 0.0 0.0 0.0 0.0 86-87 1.7875 0.0 0.0 0.0 0.0 88-89 2.0125 0.0 0.0 0.0 0.0 90-91 2.325 0.0 0.0 0.0 0.0 92-93 2.75 0.0 0.0 0.0 0.0 94-95 3.1875 0.0 0.0 0.0 0.0 96-97 3.7125000000000004 0.0 0.0 0.0 0.0 98-99 4.4625 0.0 0.0 0.0 0.0 100-101 4.9625 0.0 0.0 0.0 0.0 102-103 5.575 0.0 0.0 0.0 0.0 104-105 6.2625 0.0 0.0 0.0 0.0 106-107 6.9 0.0 0.0 0.0 0.0 108-109 7.6875 0.0 0.0 0.0 0.0 110-111 8.375 0.0 0.0 0.0 0.0 112-113 8.975000000000001 0.0 0.0 0.0 0.0 114-115 9.6625 0.0 0.0 0.0 0.0 116-117 10.162500000000001 0.0 0.0 0.0 0.0 118-119 11.0375 0.0 0.0 0.0 0.0 120-121 11.75 0.0 0.0 0.0 0.0 122-123 12.525 0.0 0.0 0.0 0.0 124-125 13.2375 0.0 0.0 0.0 0.0 126-127 14.175 0.0 0.0 0.0 0.0 128-129 14.9375 0.0 0.0 0.0 0.0 130-131 15.7625 0.0 0.0 0.0 0.0 132-133 16.362499999999997 0.0 0.0 0.0 0.0 134-135 17.049999999999997 0.0 0.0 0.0 0.0 136-137 17.700000000000003 0.0 0.0 0.0 0.0 138-139 18.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGGGG 45 2.4877938E-5 32.22222 145 >>END_MODULE SRR13165372 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165372_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.12025 37.0 37.0 37.0 37.0 37.0 2 36.2595 37.0 37.0 37.0 37.0 37.0 3 36.1865 37.0 37.0 37.0 37.0 37.0 4 36.299 37.0 37.0 37.0 37.0 37.0 5 36.372 37.0 37.0 37.0 37.0 37.0 6 36.305 37.0 37.0 37.0 37.0 37.0 7 36.2335 37.0 37.0 37.0 37.0 37.0 8 36.234 37.0 37.0 37.0 37.0 37.0 9 36.2955 37.0 37.0 37.0 37.0 37.0 10-14 36.2887 37.0 37.0 37.0 37.0 37.0 15-19 36.2305 37.0 37.0 37.0 37.0 37.0 20-24 36.18955 37.0 37.0 37.0 37.0 37.0 25-29 36.1383 37.0 37.0 37.0 37.0 37.0 30-34 36.11295 37.0 37.0 37.0 37.0 37.0 35-39 36.1161 37.0 37.0 37.0 37.0 37.0 40-44 36.100100000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.11215 37.0 37.0 37.0 37.0 37.0 50-54 36.00855 37.0 37.0 37.0 37.0 37.0 55-59 36.0342 37.0 37.0 37.0 37.0 37.0 60-64 35.970600000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.02765 37.0 37.0 37.0 37.0 37.0 70-74 35.94500000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.9273 37.0 37.0 37.0 37.0 37.0 80-84 35.892250000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.834050000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.7716 37.0 37.0 37.0 37.0 37.0 95-99 35.78375 37.0 37.0 37.0 37.0 37.0 100-104 35.754400000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.665350000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.53829999999999 37.0 37.0 37.0 37.0 37.0 115-119 35.427 37.0 37.0 37.0 37.0 37.0 120-124 35.3309 37.0 37.0 37.0 37.0 37.0 125-129 35.26715 37.0 37.0 37.0 37.0 37.0 130-134 35.03685 37.0 37.0 37.0 27.4 37.0 135-139 34.912699999999994 37.0 37.0 37.0 27.4 37.0 140-144 34.5356 37.0 37.0 37.0 25.0 37.0 145-149 34.2934 37.0 37.0 37.0 25.0 37.0 150-151 34.075625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 5.0 14 7.0 15 4.0 16 3.0 17 2.0 18 4.0 19 2.0 20 4.0 21 3.0 22 8.0 23 4.0 24 2.0 25 3.0 26 9.0 27 11.0 28 16.0 29 21.0 30 33.0 31 50.0 32 75.0 33 115.0 34 247.0 35 531.0 36 2576.0 37 264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.56461731493099 20.0 6.925972396486825 22.50941028858218 2 30.625000000000004 20.849999999999998 25.575 22.95 3 24.075 24.224999999999998 28.475 23.225 4 26.325 30.125 20.724999999999998 22.825 5 29.375 32.75 17.7 20.175 6 24.75 34.975 17.724999999999998 22.55 7 23.35 20.025000000000002 32.800000000000004 23.825 8 23.3 22.675 23.425 30.599999999999998 9 24.349999999999998 22.025 26.0 27.625 10-14 27.215 25.52 22.365 24.9 15-19 27.21 24.95 23.155 24.685000000000002 20-24 26.291572893223307 25.06626656664166 23.20580145036259 25.43635908977244 25-29 26.00300150075038 24.94247123561781 23.47173586793397 25.58279139569785 30-34 26.039113689791428 24.178462461861653 23.9183714300005 25.86405241834642 35-39 26.4629388816645 24.74742422726818 23.507052115634693 25.282584775432632 40-44 26.19547819127651 24.709883953581432 23.45438175270108 25.64025610244098 45-49 27.06447256539789 23.853348672035214 23.54824188465963 25.533936877907266 50-54 26.516629157289323 24.776194048512128 23.360840210052515 25.346336584146034 55-59 26.923461730865434 24.64232116058029 23.45672836418209 24.977488744372188 60-64 26.20786235870761 24.75742722816845 24.462338701610484 24.572371711513455 65-69 26.904035605340802 24.38865829874481 23.27349102365355 25.433815072260842 70-74 26.103051525762883 24.59729864932466 24.447223611805903 24.852426213106554 75-79 26.355542216886757 24.839935974389757 23.749499799919967 25.055022008803522 80-84 26.16654163540885 25.676419104776194 23.3408352088022 24.816204051012754 85-89 27.177229753389025 24.53103896753539 23.43054374468511 24.861187534390474 90-94 27.238171451435434 25.367610283084925 23.06191857557267 24.33229968990697 95-99 26.536634158539634 24.23605901475369 23.740935233808454 25.486371592898227 100-104 27.083541770885443 24.772386193096548 23.92696348174087 24.21710855427714 105-109 26.757040668300736 25.256365364413984 23.44054824671102 24.54604572057426 110-114 27.87336200860258 25.072521756526957 23.55706712013604 23.49704911473442 115-119 28.881552621048417 24.674869947979193 22.769107643057225 23.674469787915168 120-124 28.544272136068034 25.70785392696348 23.08654327163582 22.661330665332667 125-129 28.94802661197539 25.496473413035865 22.95032764744135 22.605172327547397 130-134 30.201610885987296 24.588523688028417 22.4973735554555 22.71249187052879 135-139 30.99549774887444 25.097548774387196 21.69584792396198 22.211105552776388 140-144 30.594178253476045 24.387316194858457 23.071921576472942 21.946583975192556 145-149 31.43071535767884 24.37718859429715 21.9959979989995 22.196098049024513 150-151 32.28267667292057 23.72732958098812 22.338961851156974 21.651031894934334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 1.0 9 1.5 10 1.0 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 1.0 25 1.0 26 0.5 27 1.5 28 3.0 29 3.0 30 6.5 31 8.0 32 12.5 33 19.0 34 26.5 35 31.0 36 30.0 37 39.5 38 53.0 39 73.5 40 105.0 41 129.5 42 150.0 43 163.5 44 167.5 45 161.5 46 168.0 47 181.0 48 166.0 49 143.0 50 136.5 51 143.5 52 128.5 53 110.0 54 112.0 55 116.0 56 105.5 57 85.5 58 96.5 59 106.0 60 96.5 61 87.0 62 77.5 63 73.0 64 82.5 65 78.0 66 52.0 67 50.0 68 70.0 69 62.5 70 49.5 71 49.0 72 39.5 73 34.0 74 23.0 75 21.5 76 18.0 77 10.0 78 6.5 79 3.5 80 2.5 81 1.5 82 0.5 83 0.5 84 1.0 85 0.5 86 0.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.5 99 1.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.015 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.045 130-134 0.055 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.55 #Duplication Level Percentage of deduplicated Percentage of total 1 73.89937106918238 52.87500000000001 2 17.26065688329839 24.7 3 5.83508036338225 12.525 4 1.9217330538085255 5.5 5 0.6988120195667366 2.5 6 0.2445842068483578 1.05 7 0.034940600978336823 0.17500000000000002 8 0.034940600978336823 0.2 9 0.034940600978336823 0.22499999999999998 >10 0.034940600978336823 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 10 0.25 No Hit GAATCCATTGTAGTGCTAAACATAAGTGGTGCTGAACAGCTGCTTGTTGC 9 0.22499999999999998 No Hit CCGCAAAGCGGTCAAGGCTGCGGAAGCAACAGCAACTCGAAGAACTTACC 8 0.2 No Hit AGAGAAGCCAAAGAGAGAGGGAGGGATTACAAGATGCAGGACTGGGCTGG 7 0.17500000000000002 No Hit AGAAGAAGGTGTCGAAGAAGCAGGAGGAAGGCGTGGTGACGAACAAGTAC 6 0.15 No Hit GCCTACTTCAAACAGAATAAATAGTTCCCAAGACTCTGGATGGGATGGTG 6 0.15 No Hit GCCCCTCAAGTTTGACAAGGCCTCTGCTTAAGGACTGGTACTGCTGCTGG 6 0.15 No Hit CCGCAAGGCCGCGGCGGGGCCTCTCAACGGCATCCTCGACGTCTGCGACG 6 0.15 No Hit AGCTAATGGGTGAATGGAGATTAATGGACGAACTCTTAGTATTACGAGCT 6 0.15 No Hit CTCTGATATCCCCACCACCGCACCATGACATCTATTCTATCGAGGATCTT 6 0.15 No Hit AGCACGGCATCGACAGGACCTTCGAGGTGGCGCAGAAGGTGTGGGCGGAG 6 0.15 No Hit GGGTAACTGAAGGAGTCTGACCATTGAAGTGCATGTGGGCGTTACACTTG 5 0.125 No Hit GGAGGATGATGGGTACGAGGATGTTGGATACGCTTACTGTGGGAGGGCGC 5 0.125 No Hit CCTTCCTCCGCCACAGTCCCCGCGCCACCATGGCCACGGCGACCCCGCAG 5 0.125 No Hit CCGCCGTCGCCGCAGAGCTTCCCCGAGAAGAAGCGGGGGAGCCGGCCGCC 5 0.125 No Hit GGGAGCGGCGAGTTCGAGGTGACCTACCTCGACGACGACACCCGCGTCAC 5 0.125 No Hit GTTCAGCAAGGTCGGCTTCGTCTTCCGCGAGCACAACAGCTCCCCTGGGT 5 0.125 No Hit CCTGATCCGGCCAACTTGGAGAAGTTCCTTGGAACCATACCAATGATGTT 5 0.125 No Hit CACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTC 5 0.125 No Hit CATCTATGTAAGCCTTTTAACAGGAAATGTAATGCATCAAGAAGCATGAA 5 0.125 No Hit AATCCCACCGCACAGCCCCATCCCCATCTCGCAACCTGCTTCGCTCCGGC 5 0.125 No Hit GCTGTTCTTGGGACTCTTGTGCGCTTCAAACAGCTTAAGAAATGGAATCT 5 0.125 No Hit GGAACATGGCAAACCACATGAAAGAACAGCTATCATTGATAAGTTGATTG 5 0.125 No Hit GCTCAAGACCGACGCCAGGAAGTACCGCAAGATGAAGGAGAGGCTCGTCG 5 0.125 No Hit GATTTTTATCATCAACCCGAAGGGTGAAGTAGCGATCAACAGTTCTGTTG 5 0.125 No Hit GGAAGGAGTCCAAAAACAAGATTAAAGGTTTGGTCGATTCCCTTAATGCA 5 0.125 No Hit GCCTCTTCTCGCTTGCTCTACCTGCTGCTTGCAACCATGGCACCCACCGT 5 0.125 No Hit GTGGAAAATCAACACTGATCCAGGCCTTGTTCAGAATAGTGGAGCCTTCG 5 0.125 No Hit AAAGCGAGCATTTCCCAGACCCAACTGGTCTTCGTGCTCGTTTGGAACAA 5 0.125 No Hit GTCTTGAGAGACAGAAAGAATATATGAAATCAAAACCTGAGAGAGATGCA 5 0.125 No Hit CCTGACAAGCTCTGCGACCAGGTCTCTGACGCCGTGCTTGACGCCTGCCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.1 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.1625 0.0 0.0 0.0 0.0 60-61 0.2375 0.0 0.0 0.0 0.0 62-63 0.25 0.0 0.0 0.0 0.0 64-65 0.3 0.0 0.0 0.0 0.0 66-67 0.36250000000000004 0.0 0.0 0.0 0.0 68-69 0.4375 0.0 0.0 0.0 0.0 70-71 0.5625 0.0 0.0 0.0 0.0 72-73 0.675 0.0 0.0 0.0 0.0 74-75 0.775 0.0 0.0 0.0 0.0 76-77 0.875 0.0 0.0 0.0 0.0 78-79 1.0375 0.0 0.0 0.0 0.0 80-81 1.15 0.0 0.0 0.0 0.0 82-83 1.4375 0.0 0.0 0.0 0.0 84-85 1.65 0.0 0.0 0.0 0.0 86-87 1.7374999999999998 0.0 0.0 0.0 0.0 88-89 1.9625 0.0 0.0 0.0 0.0 90-91 2.2625 0.0 0.0 0.0 0.0 92-93 2.675 0.0 0.0 0.0 0.0 94-95 3.1125 0.0 0.0 0.0 0.0 96-97 3.6375 0.0 0.0 0.0 0.0 98-99 4.387499999999999 0.0 0.0 0.0 0.0 100-101 4.887499999999999 0.0 0.0 0.0 0.0 102-103 5.5 0.0 0.0 0.0 0.0 104-105 6.1875 0.0 0.0 0.0 0.0 106-107 6.825 0.0 0.0 0.0 0.0 108-109 7.6125 0.0 0.0 0.0 0.0 110-111 8.275 0.0 0.0 0.0 0.0 112-113 8.8875 0.0 0.0 0.0 0.0 114-115 9.5875 0.0 0.0 0.0 0.0 116-117 10.087499999999999 0.0 0.0 0.0 0.0 118-119 10.9625 0.0 0.0 0.0 0.0 120-121 11.7125 0.0 0.0 0.0 0.0 122-123 12.5 0.0 0.0 0.0 0.0 124-125 13.212499999999999 0.0 0.0 0.0 0.0 126-127 14.149999999999999 0.0 0.0 0.0 0.0 128-129 14.925 0.0 0.0 0.0 0.0 130-131 15.7375 0.0 0.0 0.0 0.0 132-133 16.3375 0.0 0.0 0.0 0.0 134-135 17.025 0.0 0.0 0.0 0.0 136-137 17.674999999999997 0.0 0.0 0.0 0.0 138-139 18.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975634 spots for SRR13165372.sra Written 975634 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra Read 975618 spots for SRR13165372.sra Written 975618 spots for SRR13165372.sra SRR ids: ['SRR13165372.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rcmy7uto SRR13165372.sra spots: 19512376 blocks: [[1, 975618], [975619, 1951236], [1951237, 2926854], [2926855, 3902472], [3902473, 4878090], [4878091, 5853708], [5853709, 6829326], [6829327, 7804944], [7804945, 8780562], [8780563, 9756180], [9756181, 10731798], [10731799, 11707416], [11707417, 12683034], [12683035, 13658652], [13658653, 14634270], [14634271, 15609888], [15609889, 16585506], [16585507, 17561124], [17561125, 18536742], [18536743, 19512376]] SRR13165372 file size 6609458 SRR13165372 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165372 SRR13165372_1.fastq SRR13165372_2.fastq Input file: SRR13165372_1.fastq Paired file: SRR13165372_2.fastq trimmed: SRR13165372-trimmed-pair1.fastq, SRR13165372-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:36:52 2024 >> started Sat Dec 7 16:37:15 2024 >> done (22.156s) 19512376 read pairs processed; of these: 585 ( 0.00%) short read pairs filtered out after trimming by size control 27671 ( 0.14%) empty read pairs filtered out after trimming by size control 19484120 (99.86%) read pairs available; of these: 4045850 (20.76%) trimmed read pairs available after processing 15438270 (79.24%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 31 0.00% 19 33 0.00% 20 29 0.00% 21 43 0.00% 22 64 0.00% 23 75 0.00% 24 94 0.00% 25 72 0.00% 26 90 0.00% 27 135 0.00% 28 113 0.00% 29 128 0.00% 30 130 0.00% 31 145 0.00% 32 157 0.00% 33 144 0.00% 34 157 0.00% 35 150 0.00% 36 200 0.00% 37 180 0.00% 38 176 0.00% 39 231 0.00% 40 234 0.00% 41 248 0.00% 42 258 0.00% 43 309 0.00% 44 302 0.00% 45 353 0.00% 46 356 0.00% 47 396 0.00% 48 457 0.00% 49 499 0.00% 50 641 0.00% 51 735 0.00% 52 744 0.00% 53 890 0.00% 54 880 0.00% 55 969 0.00% 56 991 0.01% 57 1298 0.01% 58 1366 0.01% 59 1524 0.01% 60 1698 0.01% 61 1938 0.01% 62 2336 0.01% 63 2608 0.01% 64 2613 0.01% 65 2978 0.02% 66 3275 0.02% 67 3500 0.02% 68 3995 0.02% 69 4613 0.02% 70 5008 0.03% 71 5814 0.03% 72 6574 0.03% 73 7499 0.04% 74 8257 0.04% 75 9009 0.05% 76 9262 0.05% 77 10168 0.05% 78 11398 0.06% 79 12579 0.06% 80 13643 0.07% 81 15016 0.08% 82 16896 0.09% 83 18951 0.10% 84 20872 0.11% 85 21900 0.11% 86 22364 0.11% 87 23663 0.12% 88 24775 0.13% 89 26579 0.14% 90 28498 0.15% 91 30385 0.16% 92 32393 0.17% 93 34155 0.18% 94 35959 0.18% 95 37998 0.20% 96 39373 0.20% 97 40445 0.21% 98 41196 0.21% 99 43345 0.22% 100 44279 0.23% 101 46020 0.24% 102 47724 0.24% 103 48684 0.25% 104 50405 0.26% 105 51428 0.26% 106 52847 0.27% 107 53785 0.28% 108 54793 0.28% 109 55309 0.28% 110 54994 0.28% 111 56320 0.29% 112 58726 0.30% 113 60180 0.31% 114 61833 0.32% 115 62475 0.32% 116 63201 0.32% 117 63819 0.33% 118 63699 0.33% 119 64081 0.33% 120 64782 0.33% 121 65520 0.34% 122 65421 0.34% 123 67582 0.35% 124 69107 0.35% 125 68384 0.35% 126 69880 0.36% 127 69357 0.36% 128 68488 0.35% 129 69914 0.36% 130 69611 0.36% 131 70264 0.36% 132 71918 0.37% 133 71420 0.37% 134 71754 0.37% 135 73538 0.38% 136 73018 0.37% 137 71710 0.37% 138 72381 0.37% 139 73308 0.38% 140 73500 0.38% 141 73704 0.38% 142 74338 0.38% 143 73650 0.38% 144 74395 0.38% 145 76716 0.39% 146 77513 0.40% 147 80761 0.41% 148 76979 0.40% 149 77556 0.40% 150 75224 0.39% 151 15438270 79.24% 19484120 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=34 prefix-density=0.47 prefix-fanout=2.0 sequence=TAGGCGTCCGGGTACTCCTTCTTGACCTCCTCCAGCTCCTTGAGCACCTGTGTGGCGTCGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=101.77 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=8.8 sequence=GCATCAATGATAATCCCAAAGTAGGAAGTGTGTACGTAGTAGCAAGTGTTATACATACATAAAATTAAGCGATGCGTTTCGATCAAGTACTTGGCATCATCGATCACATACGTACATTTCACAGCAGGTAGCTACGTACATTACAGGCATACGTACATGCAGAGAGATACCCGGCCCTGTGTTGTGTTTGCAATTGCATAGATGAGAATGAGATGACTTGATTCTTAATTAGCCGGCGAGAGCGCTGGTGTTGTAGACGAGGAGGGCGCCGCCGCCGAGGAGGCCGGCCAGGGTGACGGCCCAGATCAGGAGCCCCGTCGTCCCACCGGCGTAGCGGTCGCCAGATTCGGACCATACCTCCGGCGTGTAGATCGGGCTGTAGCCGTCGACGTTGGCGCCGTACTTGTCAACAAACTGGTACACACCCTTTCCCGTGCCCTTCCTGCCGTTGGCGTCGATGTCCACCGTCAAGCCACCTCCGATCCCGAGGGGCTTGTCGACCTTGATCTTC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=3.41 fanout-score-rank=23 prefix-density=0.38 prefix-fanout=3.0 sequence=GAGTTCAGCAAGGTCGGCTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=30 fanout-score=90.73 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=8.9 sequence=GCAGCAGCCATTACTTGATCATCTGAAAAATCTTAATCCAGATCAGACCAAGCAGAGCAGAGATGTCTGCTACCTTCTCGTCCACCGTCGGAGCTCCGGCTTCTACGCCAACCAGCTTCCTTGGGAAGAAGCTCAAGAAGCAGGTGACCTCGGCCGTGAACTACCATGGCAAGAGCACCAAGGCCAACAGATTCACAGTCATGGCCAAGGAGGTGGACGAGTCAAAGCAGACTGACCAGGACAGGTGGAAGGGCCTCGCCTACGATATCTCCGACGACCAGCAGGACATCACCAGGGGGAAGGGTATCGTCGACTCGCTCTTCCAGGCGCCCATGGGCGACGGTACCCACGTGGCCGTCCTCAGCTCCCAAGAGTACATCAGCCAGGGCCTAAGGAAGTACGACTTCGACAACA SRR13165372 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:38:54 Started mapping on | Dec 07 16:38:54 Finished on | Dec 07 16:40:24 Mapping speed, Million of reads per hour | 779.36 Number of input reads | 19484120 Average input read length | 281 UNIQUE READS: Uniquely mapped reads number | 15317511 Uniquely mapped reads % | 78.62% Average mapped length | 285.93 Number of splices: Total | 15759140 Number of splices: Annotated (sjdb) | 14802829 Number of splices: GT/AG | 15557827 Number of splices: GC/AG | 169041 Number of splices: AT/AC | 7617 Number of splices: Non-canonical | 24655 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.01% Deletion average length | 2.91 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 155861 % of reads mapped to multiple loci | 0.80% Number of reads mapped to too many loci | 28444 % of reads mapped to too many loci | 0.15% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 19.93% % of reads unmapped: other | 0.51% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4010968 4010968 4010968 N_multimapping 155861 155861 155861 N_noFeature 495385 14917384 635891 N_ambiguous 345692 2980 86867 UnstrandedReadsAssigned:14476434 PositiveStrandReadsAssigned:397147 NegativeStrandReadsAssigned:14594753 Dataset is classified negative stranded MeadianReadLen=143 20thPercentileLength=141 echo kmer=137 SRR13165372 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165372-trimmed-pair1.fastq SRR13165372-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,484,120 reads, 17,849,197 reads pseudoaligned [quant] estimated average fragment length: 235.031 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,173 rounds 52973 SRR13165372.ke.tsv 35125 SRR13165372.se.tsv 88098 total ==> SRR13165372.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 702.469 0 0 PNS24247 1044 809.968 43.9706 4.57324 PNS24249 1928 1693.97 106.456 5.29412 PNS24246 1044 809.968 43.9706 4.57324 PNS24248 1044 809.968 43.9706 4.57324 PNS24244 1471 1236.97 69.6321 4.74221 PNS24243 293 119.879 0 0 KQK14069 1603 1368.97 427.811 26.3262 KQK14071 474 262.156 6.67261 2.1442 ==> SRR13165372.se.tsv <== BRADI_1g14170v3 367 BRADI_1g53295v3 103 BRADI_1g59795v3 327 BRADI_1g07683v3 0 BRADI_1g00485v3 9 BRADI_1g20270v3 434 BRADI_1g74790v3 258 BRADI_1g09890v3 0 BRADI_1g77505v3 185 BRADI_1g48960v3 0 SRR13165372 completed mapping pipeline successfully