Starting /dee2/code/volunteer_pipeline.sh SRR13165373 current disk space = 1541747359744 free memory = 1602317196 SRR13165373 SRAfilesize fa8026d451526c64be6b3d633a0a46ab SRR13165373.sra SRR13165373.sra file validated SRR13165373 is paired end SRR13165373 is conventional basespace SRR13165373 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165373_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5615 37.0 37.0 37.0 37.0 37.0 2 36.168 37.0 37.0 37.0 37.0 37.0 3 36.4275 37.0 37.0 37.0 37.0 37.0 4 36.57 37.0 37.0 37.0 37.0 37.0 5 36.595 37.0 37.0 37.0 37.0 37.0 6 36.5405 37.0 37.0 37.0 37.0 37.0 7 36.393 37.0 37.0 37.0 37.0 37.0 8 36.4795 37.0 37.0 37.0 37.0 37.0 9 36.5515 37.0 37.0 37.0 37.0 37.0 10-14 36.584 37.0 37.0 37.0 37.0 37.0 15-19 36.5283 37.0 37.0 37.0 37.0 37.0 20-24 36.5022 37.0 37.0 37.0 37.0 37.0 25-29 36.5033 37.0 37.0 37.0 37.0 37.0 30-34 36.466699999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.4146 37.0 37.0 37.0 37.0 37.0 40-44 36.3438 37.0 37.0 37.0 37.0 37.0 45-49 35.51819999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.658699999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.1212 37.0 37.0 37.0 37.0 37.0 60-64 35.2586 37.0 37.0 37.0 37.0 37.0 65-69 35.1817 37.0 37.0 37.0 37.0 37.0 70-74 35.4508 37.0 37.0 37.0 37.0 37.0 75-79 36.18770000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.234899999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.25 37.0 37.0 37.0 37.0 37.0 90-94 36.1821 37.0 37.0 37.0 37.0 37.0 95-99 36.2117 37.0 37.0 37.0 37.0 37.0 100-104 36.138799999999996 37.0 37.0 37.0 37.0 37.0 105-109 36.124399999999994 37.0 37.0 37.0 37.0 37.0 110-114 36.13009999999999 37.0 37.0 37.0 37.0 37.0 115-119 36.0869 37.0 37.0 37.0 37.0 37.0 120-124 35.9342 37.0 37.0 37.0 37.0 37.0 125-129 35.9345 37.0 37.0 37.0 37.0 37.0 130-134 35.8801 37.0 37.0 37.0 37.0 37.0 135-139 35.7971 37.0 37.0 37.0 37.0 37.0 140-144 35.542199999999994 37.0 37.0 37.0 37.0 37.0 145-149 35.32340000000001 37.0 37.0 37.0 37.0 37.0 150-151 35.0475 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 0.0 20 1.0 21 0.0 22 0.0 23 4.0 24 3.0 25 3.0 26 8.0 27 4.0 28 12.0 29 21.0 30 31.0 31 36.0 32 130.0 33 217.0 34 142.0 35 305.0 36 2688.0 37 393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.300000000000004 8.25 6.7250000000000005 31.724999999999998 2 22.92400603925516 14.796175138399597 30.77503774534474 31.504781077000505 3 18.95 13.5 28.425 39.125 4 26.325 19.175 21.975 32.525 5 30.375000000000004 25.0 21.375 23.25 6 28.925 30.2 19.475 21.4 7 18.975 29.025000000000002 34.725 17.275 8 19.8 28.449999999999996 26.650000000000002 25.1 9 25.575 19.6 31.2 23.625 10-14 22.85 27.435 24.349999999999998 25.365 15-19 23.849999999999998 24.385 25.215 26.55 20-24 23.18 26.345000000000002 25.105 25.369999999999997 25-29 23.62 25.255 25.245 25.88 30-34 23.235 24.805 24.875 27.084999999999997 35-39 22.814999999999998 26.085 25.385 25.715 40-44 24.865000000000002 24.709999999999997 24.43 25.995 45-49 24.785 23.535 25.94 25.740000000000002 50-54 25.09 24.175 24.205 26.529999999999998 55-59 24.555 22.725 26.275 26.445 60-64 25.4 23.24 25.985000000000003 25.374999999999996 65-69 24.175 27.005000000000003 24.4 24.42 70-74 28.17 24.37 23.025000000000002 24.435000000000002 75-79 28.88 23.22 23.119999999999997 24.779999999999998 80-84 28.405 24.14 22.220000000000002 25.235000000000003 85-89 28.675 22.96 23.119999999999997 25.245 90-94 28.904999999999998 23.365 23.01 24.72 95-99 29.415000000000003 23.105 23.064999999999998 24.415 100-104 29.015 23.775 22.305 24.905 105-109 29.665000000000003 24.235 22.27 23.830000000000002 110-114 29.060000000000002 23.849999999999998 22.115000000000002 24.975 115-119 28.71 24.240000000000002 22.695 24.355 120-124 28.67 23.895 22.325 25.11 125-129 28.939999999999998 24.085 22.835 24.14 130-134 29.095 23.375 22.869999999999997 24.66 135-139 28.37 24.445 22.275 24.91 140-144 29.4 22.895 23.064999999999998 24.64 145-149 29.715000000000003 22.86 22.665 24.759999999999998 150-151 30.049999999999997 22.475 23.1625 24.3125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 0.5 25 0.5 26 0.0 27 0.0 28 1.0 29 4.0 30 5.5 31 10.0 32 17.0 33 19.0 34 21.5 35 33.0 36 47.0 37 51.5 38 65.0 39 92.5 40 110.5 41 122.5 42 148.0 43 188.5 44 184.0 45 154.0 46 165.5 47 165.5 48 142.5 49 135.5 50 140.5 51 135.0 52 142.0 53 139.5 54 117.0 55 98.5 56 84.5 57 67.5 58 65.5 59 80.0 60 82.5 61 83.5 62 71.0 63 59.5 64 65.5 65 92.0 66 100.5 67 86.5 68 93.5 69 83.5 70 54.0 71 38.0 72 26.0 73 24.5 74 22.5 75 17.0 76 13.5 77 11.0 78 8.0 79 3.0 80 2.5 81 2.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.65 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.75 #Duplication Level Percentage of deduplicated Percentage of total 1 74.51612903225806 51.975 2 17.096774193548388 23.849999999999998 3 5.412186379928316 11.325000000000001 4 1.935483870967742 5.4 5 0.5734767025089605 2.0 6 0.10752688172043011 0.44999999999999996 7 0.035842293906810034 0.17500000000000002 8 0.0 0.0 9 0.035842293906810034 0.22499999999999998 >10 0.25089605734767023 3.2750000000000004 >50 0.035842293906810034 1.325 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCTCGTAT 53 1.325 TruSeq Adapter, Index 11 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCTCGTTT 32 0.8 TruSeq Adapter, Index 11 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCTCGGAT 28 0.7000000000000001 TruSeq Adapter, Index 11 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCTCGGTT 19 0.475 TruSeq Adapter, Index 11 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCGCGTTT 16 0.4 TruSeq Adapter, Index 11 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCGCGTAT 15 0.375 TruSeq Adapter, Index 11 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCGCGGAT 11 0.27499999999999997 TruSeq Adapter, Index 11 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCGCGGTT 10 0.25 TruSeq Adapter, Index 11 (97% over 37bp) GCCTCTAGGAATTTCAACCACACAGTTGAAAACTGCAGGAGCTCCTGGAC 9 0.22499999999999998 No Hit GCCACATACAAGAACCGGCTAGCAAATACTCCAGAACACAAAAAATTGAA 7 0.17500000000000002 No Hit GCTTACCGATCCATCGACTTTTTTAACTGATTACAATGCGAATGCAAATG 6 0.15 No Hit TCGGCACTGACATCCTACTACAGATGACTAGTATTAGTAATACTGTTGAT 6 0.15 No Hit GCCCCGACCAGCCGCCGCCACGCCCGACCTGCCCCGAACACGCCCTAGCT 6 0.15 No Hit GCTTGAGCTTGTAGAGGATGGTGGTCTTCCCGGCGGCGTCGAGCCCCACC 5 0.125 No Hit TGTGTATCTGAGTGGTGTCCTGTACTCCGCAGTTGGTGATTCTGAAAGAG 5 0.125 No Hit GCATCAACAAGCTTTGCGCCTTGGGCAAAAATCCGACCAGCACCAAGAGC 5 0.125 No Hit CCCACGGGATGATATAGATCGCTGCTGTTGAAACTGGAGATGGGCCAGAG 5 0.125 No Hit CGCTGTTGGTGATCTCAGCAACAGAAAGCTGCTCATGGTAAGCCTTCTCA 5 0.125 No Hit GTATCATTCCAACTAAGTAAAATGGCTCATTGTCACAAGGAGAACGCTAA 5 0.125 No Hit GTCCAGCCATATTTTCAGATAAATTTTTTCCATCGATCTTTTCTGGTTCA 5 0.125 No Hit GGCAAGTTCATTGAGATGGCAGGGGTGTTTGCTCCTAGTGTTTGCCAGAA 5 0.125 No Hit GCATTCATGATTCATGATTTTTTTTAACAAAATAAGTCCGCCAAATGAAA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTTAGGATCTCGGGT 5 0.125 TruSeq Adapter, Index 11 (97% over 37bp) CTCTACTGGCCGGCAGGCCCAACAAGAACACCCTGCGCGGAGGCTTCCTT 5 0.125 No Hit CTTGTTCATTCTGGTAGGAATTATTGACATGGATAAGTATGAGTTGATCC 5 0.125 No Hit GGCTGCGCGCAAGGGGGACGTATGAGGGGCTGCGCGGTGGCCGGCGACTC 5 0.125 No Hit CCACCTAGAAGTTGATATGTTCCTCATTGTTGGGTACTCCTCTTCCTCCA 5 0.125 No Hit GCCATACTCAGTGCTGTTAAAATTCAAGAGTCCGACTTTGAGCTTTTGGT 5 0.125 No Hit ATGGAGTAGTCGCTCGTCTTGTGGAAGCACAAAGCTCTCTAGCTCGCTGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.07500000000000001 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.16249999999999998 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.1875 0.0 0.0 0.0 0.0 74-75 0.2375 0.0 0.0 0.0 0.0 76-77 0.4 0.0 0.0 0.0 0.0 78-79 0.55 0.0 0.0 0.0 0.0 80-81 0.6375 0.0 0.0 0.0 0.0 82-83 0.6875 0.0 0.0 0.0 0.0 84-85 0.7875000000000001 0.0 0.0 0.0 0.0 86-87 0.8500000000000001 0.0 0.0 0.0 0.0 88-89 1.0125 0.0 0.0 0.0 0.0 90-91 1.3625 0.0 0.0 0.0 0.0 92-93 1.825 0.0 0.0 0.0 0.0 94-95 2.2249999999999996 0.0 0.0 0.0 0.0 96-97 2.625 0.0 0.0 0.0 0.0 98-99 2.95 0.0 0.0 0.0 0.0 100-101 3.5 0.0 0.0 0.0 0.0 102-103 3.8625 0.0 0.0 0.0 0.0 104-105 4.3125 0.0 0.0 0.0 0.0 106-107 5.0625 0.0 0.0 0.0 0.0 108-109 5.7125 0.0 0.0 0.0 0.0 110-111 6.2125 0.0 0.0 0.0 0.0 112-113 6.725 0.0 0.0 0.0 0.0 114-115 7.1875 0.0 0.0 0.0 0.0 116-117 7.775 0.0 0.0 0.0 0.0 118-119 8.3375 0.0 0.0 0.0 0.0 120-121 9.2125 0.0 0.0 0.0 0.0 122-123 10.125 0.0 0.0 0.0 0.0 124-125 10.575 0.0 0.0 0.0 0.0 126-127 11.0625 0.0 0.0 0.0 0.0 128-129 11.55 0.0 0.0 0.0 0.0 130-131 12.225000000000001 0.0 0.0 0.0 0.0 132-133 12.8875 0.0 0.0 0.0 0.0 134-135 13.6 0.0 0.0 0.0 0.0 136-137 14.2625 0.0 0.0 0.0 0.0 138-139 14.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCAG 10 0.006830828 145.0 9 CTCTACT 10 0.006830828 145.0 1 CTGGCCG 10 0.006830828 145.0 6 ATATGCA 10 0.006830828 145.0 8 GCTTGCA 10 0.006830828 145.0 2 AGCGCTT 10 0.006830828 145.0 145 ACTGGCC 10 0.006830828 145.0 5 CATATGC 10 0.006830828 145.0 7 CTACTGG 10 0.006830828 145.0 3 GCATATG 15 1.1411342E-4 145.0 6 GATCCTG 20 0.00593511 29.0 90-94 >>END_MODULE SRR13165373 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165373_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0985 37.0 37.0 37.0 37.0 37.0 2 36.116 37.0 37.0 37.0 37.0 37.0 3 35.9595 37.0 37.0 37.0 37.0 37.0 4 36.0075 37.0 37.0 37.0 37.0 37.0 5 36.087 37.0 37.0 37.0 37.0 37.0 6 36.0695 37.0 37.0 37.0 37.0 37.0 7 35.8755 37.0 37.0 37.0 37.0 37.0 8 35.5945 37.0 37.0 37.0 37.0 37.0 9 35.545 37.0 37.0 37.0 37.0 37.0 10-14 35.4208 37.0 37.0 37.0 37.0 37.0 15-19 35.4201 37.0 37.0 37.0 37.0 37.0 20-24 35.266000000000005 37.0 37.0 37.0 37.0 37.0 25-29 34.85080000000001 37.0 37.0 37.0 27.4 37.0 30-34 34.7242 37.0 37.0 37.0 25.0 37.0 35-39 34.623900000000006 37.0 37.0 37.0 25.0 37.0 40-44 34.744299999999996 37.0 37.0 37.0 25.0 37.0 45-49 34.6146 37.0 37.0 37.0 25.0 37.0 50-54 34.589 37.0 37.0 37.0 25.0 37.0 55-59 34.803399999999996 37.0 37.0 37.0 25.0 37.0 60-64 34.917899999999996 37.0 37.0 37.0 25.0 37.0 65-69 34.8332 37.0 37.0 37.0 25.0 37.0 70-74 34.48819999999999 37.0 37.0 37.0 25.0 37.0 75-79 34.449200000000005 37.0 37.0 37.0 25.0 37.0 80-84 34.69540000000001 37.0 37.0 37.0 25.0 37.0 85-89 35.1194 37.0 37.0 37.0 32.2 37.0 90-94 35.27419999999999 37.0 37.0 37.0 34.6 37.0 95-99 35.374700000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.470699999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.4814 37.0 37.0 37.0 37.0 37.0 110-114 35.3384 37.0 37.0 37.0 34.6 37.0 115-119 35.327600000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.148700000000005 37.0 37.0 37.0 34.6 37.0 125-129 35.2378 37.0 37.0 37.0 34.6 37.0 130-134 35.0179 37.0 37.0 37.0 27.4 37.0 135-139 34.83635 37.0 37.0 37.0 25.0 37.0 140-144 34.5841 37.0 37.0 37.0 25.0 37.0 145-149 34.45255 37.0 37.0 37.0 25.0 37.0 150-151 34.2275 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 8.0 14 10.0 15 9.0 16 8.0 17 8.0 18 3.0 19 16.0 20 7.0 21 20.0 22 22.0 23 24.0 24 27.0 25 19.0 26 39.0 27 43.0 28 47.0 29 40.0 30 36.0 31 45.0 32 72.0 33 115.0 34 233.0 35 534.0 36 2396.0 37 218.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.090225563909776 19.072681704260653 8.696741854636592 26.14035087719298 2 32.85 20.95 25.825 20.375 3 28.725 22.625 25.674999999999997 22.975 4 29.925 26.85 19.650000000000002 23.575 5 31.574999999999996 28.999999999999996 18.15 21.275 6 28.975 31.374999999999996 18.3 21.349999999999998 7 28.225 19.075 29.799999999999997 22.900000000000002 8 27.875 22.325 21.925 27.875 9 27.3 21.125 25.624999999999996 25.95 10-14 30.320000000000004 23.86 21.25 24.57 15-19 30.035 23.27 22.575 24.12 20-24 29.520000000000003 23.01 22.805 24.665 25-29 29.2 22.7 22.6 25.5 30-34 28.96 23.82 23.275000000000002 23.945 35-39 28.12 23.835 23.035 25.009999999999998 40-44 28.860000000000003 23.724999999999998 23.14 24.275 45-49 26.840000000000003 24.25 24.25 24.66 50-54 28.685 23.555 23.755000000000003 24.005000000000003 55-59 29.885 23.135 23.22 23.76 60-64 29.725 23.57 22.57 24.135 65-69 29.45 23.905 22.615 24.03 70-74 28.92 24.125 23.205000000000002 23.75 75-79 28.935 24.055 22.915 24.095 80-84 29.054999999999996 23.785 23.405 23.755000000000003 85-89 30.599999999999998 23.73 22.555 23.115 90-94 30.564999999999998 23.535 22.465 23.435 95-99 30.349999999999998 23.674999999999997 22.465 23.51 100-104 30.785 24.585 21.85 22.78 105-109 30.354999999999997 23.5 23.31 22.835 110-114 31.855 23.51 22.645 21.990000000000002 115-119 32.105 24.52 21.14 22.235 120-124 32.195 24.455 21.84 21.51 125-129 32.1 23.47 22.31 22.12 130-134 34.07840784078408 23.50735073507351 21.507150715071507 20.907090709070907 135-139 33.521676083804195 23.611180559027954 22.3861193059653 20.48102405120256 140-144 34.335 24.38 21.305 19.98 145-149 34.366718335916794 23.62618130906545 21.741087054352718 20.266013300665033 150-151 35.33383345836459 23.53088272068017 22.193048262065513 18.942235558889724 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.0 5 0.5 6 0.5 7 1.0 8 1.0 9 0.5 10 1.5 11 1.5 12 1.0 13 1.0 14 0.5 15 0.5 16 0.5 17 0.0 18 1.0 19 1.0 20 0.5 21 1.0 22 2.5 23 2.0 24 0.0 25 0.5 26 1.0 27 2.5 28 4.0 29 4.5 30 10.5 31 15.5 32 11.5 33 14.5 34 24.5 35 33.5 36 42.0 37 46.0 38 57.5 39 84.0 40 92.5 41 102.0 42 118.5 43 144.0 44 150.0 45 142.0 46 148.0 47 147.0 48 151.5 49 146.5 50 138.0 51 132.5 52 127.5 53 119.0 54 123.5 55 119.0 56 104.5 57 96.0 58 90.5 59 94.0 60 85.5 61 76.0 62 79.0 63 79.0 64 75.0 65 73.5 66 61.0 67 64.0 68 66.5 69 45.0 70 37.5 71 34.0 72 36.5 73 33.5 74 24.0 75 17.5 76 11.0 77 11.0 78 10.5 79 9.5 80 6.5 81 3.5 82 4.0 83 4.5 84 5.0 85 4.5 86 5.5 87 8.0 88 8.0 89 7.5 90 6.5 91 9.5 92 11.0 93 13.5 94 15.0 95 14.0 96 18.5 97 16.5 98 14.5 99 13.0 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.01 135-139 0.005 140-144 0.0 145-149 0.005 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.15 #Duplication Level Percentage of deduplicated Percentage of total 1 76.66891436277815 56.85 2 16.11598111935266 23.9 3 4.551584625758597 10.125 4 1.8880647336480108 5.6000000000000005 5 0.5731625084288604 2.125 6 0.10114632501685772 0.44999999999999996 7 0.033715441672285906 0.17500000000000002 8 0.0 0.0 9 0.033715441672285906 0.22499999999999998 >10 0.033715441672285906 0.5499999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 22 0.5499999999999999 No Hit GCTGGAGAAGCTGATGGGAACACCCGACAGACATCAAGGTTCCCCCCGGC 9 0.22499999999999998 No Hit CCCTGCAAGAAAAGGGGTTCATCCAGAAGGCTTCTGGCATCAAGGAGCTT 7 0.17500000000000002 No Hit GGAAACGCAGCCCAGGAAGCAACAGCCTCATCTCCCCCCCTCCCAAGAAC 6 0.15 No Hit GCGGAAGCCAGGAGAGCCAGCCCGTGCACAACGGGCAGCTGCCGCACTCA 6 0.15 No Hit GACTATCATGCCCAAGGACATTCAGCTGGCTAGGAGGATCCGTGGCGAGA 6 0.15 No Hit GTTCTTCTTGCTCCGCAAACCACAGGAAGAAAAGCGAAAGCAAAGGCGAT 5 0.125 No Hit GAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAA 5 0.125 No Hit CGGGAAGGAGAGGTCCAAGAGGAGGCAGGGTCCAGAGAGGGGTTGGAGAG 5 0.125 No Hit TCTGAAAACCGAAACCTATTTCCACTTGTTTGCAGGGACATGGGGGGAAG 5 0.125 No Hit CTAAAGCAGGTCAAGTCCTGCAAGCAATTGAAGTACCTGAGCTTCAGAGG 5 0.125 No Hit GGCCAGTTGAATCCCCATCAACATTCAGCAAAGTAAGTATTCGTGATGGA 5 0.125 No Hit CTCTGATATTGGCAAGACCGCTGTACAATACAAATCAGAGAGACGAGCAG 5 0.125 No Hit GTTACCGGCCACCGCTCGCTGCGCTTCCCTCTGCCCTAACCCACCAAGCG 5 0.125 No Hit CAGCGCGATTACACAATGGAACATGTCTTAAGCCAGCTGAAGAAAGATAT 5 0.125 No Hit ACCGTTCCCAGCCCCCTCGTGCGGCGGACCTCTTCGTCGCCGGCGGCGGC 5 0.125 No Hit GGAATCCTTGTCCGCTGATACAGCATCTGGGACGAAAGTGGTGGAATCCG 5 0.125 No Hit GCGGCGGCGCCGGGGGCGGAGGCGGCGACGACTTGCAACGCGCTGCAGCT 5 0.125 No Hit CGAGCACCAACTACAGTCCAGCTCTTTGTATGTGGATGTTGACTGGATAT 5 0.125 No Hit GTTAATGAGTTCCAGACCAACCTTGTGCCCTATCCAAGGATCCACTTCAT 5 0.125 No Hit GTTTAATGACATTTGAGCTTCAGGATAAGACATTTTTTCACTTTCCTAAT 5 0.125 No Hit ATTCAGGTGATCCAGATCGATGAGGCTGCTTTAAGAGAGGGCCTTCCGCT 5 0.125 No Hit GTTGCACCAGGTTCTGACATCATATTCACCGATGATGTTAGCTTCCAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1375 0.0 0.0 0.0 0.0 74-75 0.1875 0.0 0.0 0.0 0.0 76-77 0.35 0.0 0.0 0.0 0.0 78-79 0.5 0.0 0.0 0.0 0.0 80-81 0.6 0.0 0.0 0.0 0.0 82-83 0.6625000000000001 0.0 0.0 0.0 0.0 84-85 0.7625 0.0 0.0 0.0 0.0 86-87 0.825 0.0 0.0 0.0 0.0 88-89 0.9874999999999999 0.0 0.0 0.0 0.0 90-91 1.375 0.0 0.0 0.0 0.0 92-93 1.875 0.0 0.0 0.0 0.0 94-95 2.2625 0.0 0.0 0.0 0.0 96-97 2.675 0.0 0.0 0.0 0.0 98-99 3.0 0.0 0.0 0.0 0.0 100-101 3.575 0.0 0.0 0.0 0.0 102-103 3.9375 0.0 0.0 0.0 0.0 104-105 4.3875 0.0 0.0 0.0 0.0 106-107 5.1375 0.0 0.0 0.0 0.0 108-109 5.8125 0.0 0.0 0.0 0.0 110-111 6.3625 0.0 0.0 0.0 0.0 112-113 6.875 0.0 0.0 0.0 0.0 114-115 7.362500000000001 0.0 0.0 0.0 0.0 116-117 7.95 0.0 0.0 0.0 0.0 118-119 8.55 0.0 0.0 0.0 0.0 120-121 9.4125 0.0 0.0 0.0 0.0 122-123 10.25 0.0 0.0 0.0 0.0 124-125 10.7 0.0 0.0 0.0 0.0 126-127 11.1875 0.0 0.0 0.0 0.0 128-129 11.675 0.0 0.0 0.0 0.0 130-131 12.3375 0.0 0.0 0.0 0.0 132-133 12.9875 0.0 0.0 0.0 0.0 134-135 13.7 0.0 0.0 0.0 0.0 136-137 14.3625 0.0 0.0 0.0 0.0 138-139 14.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACCCC 10 0.006830828 145.0 3 ATGAATG 10 0.006830828 145.0 3 AAGGGGA 10 0.006830828 145.0 145 CCCATCA 10 0.006830828 145.0 7 CCGCAAC 10 0.006830828 145.0 1 GCAACCC 10 0.006830828 145.0 2 CCATCAG 10 0.006830828 145.0 8 CTTCGAG 10 0.006830828 145.0 5 CTATGAA 10 0.006830828 145.0 1 TGAATGA 10 0.006830828 145.0 4 CGCAACA 10 0.006830828 145.0 2 AATGATG 20 3.5877043E-4 108.75 6 AAAAAAA 20 0.00593511 29.0 70-74 >>END_MODULE Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572474 spots for SRR13165373.sra Written 1572474 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra Read 1572467 spots for SRR13165373.sra Written 1572467 spots for SRR13165373.sra SRR ids: ['SRR13165373.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0lnlbj7x SRR13165373.sra spots: 31449347 blocks: [[1, 1572467], [1572468, 3144934], [3144935, 4717401], [4717402, 6289868], [6289869, 7862335], [7862336, 9434802], [9434803, 11007269], [11007270, 12579736], [12579737, 14152203], [14152204, 15724670], [15724671, 17297137], [17297138, 18869604], [18869605, 20442071], [20442072, 22014538], [22014539, 23587005], [23587006, 25159472], [25159473, 26731939], [26731940, 28304406], [28304407, 29876873], [29876874, 31449347]] SRR13165373 file size 10666163 SRR13165373 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165373 SRR13165373_1.fastq SRR13165373_2.fastq Input file: SRR13165373_1.fastq Paired file: SRR13165373_2.fastq trimmed: SRR13165373-trimmed-pair1.fastq, SRR13165373-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:38:15 2024 >> started Sat Dec 7 16:38:50 2024 >> done (34.584s) 31449347 read pairs processed; of these: 1026 ( 0.00%) short read pairs filtered out after trimming by size control 1249074 ( 3.97%) empty read pairs filtered out after trimming by size control 30199247 (96.03%) read pairs available; of these: 5871100 (19.44%) trimmed read pairs available after processing 24328147 (80.56%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 35 0.00% 19 45 0.00% 20 69 0.00% 21 86 0.00% 22 85 0.00% 23 105 0.00% 24 90 0.00% 25 93 0.00% 26 107 0.00% 27 144 0.00% 28 138 0.00% 29 139 0.00% 30 146 0.00% 31 170 0.00% 32 187 0.00% 33 162 0.00% 34 225 0.00% 35 196 0.00% 36 218 0.00% 37 222 0.00% 38 255 0.00% 39 268 0.00% 40 286 0.00% 41 304 0.00% 42 356 0.00% 43 397 0.00% 44 413 0.00% 45 420 0.00% 46 423 0.00% 47 495 0.00% 48 630 0.00% 49 707 0.00% 50 753 0.00% 51 827 0.00% 52 962 0.00% 53 1018 0.00% 54 1157 0.00% 55 1219 0.00% 56 1380 0.00% 57 1549 0.01% 58 1747 0.01% 59 1950 0.01% 60 2334 0.01% 61 2760 0.01% 62 2955 0.01% 63 3370 0.01% 64 3861 0.01% 65 4083 0.01% 66 4508 0.01% 67 5017 0.02% 68 5529 0.02% 69 6065 0.02% 70 6920 0.02% 71 8075 0.03% 72 9159 0.03% 73 10432 0.03% 74 11412 0.04% 75 12534 0.04% 76 13647 0.05% 77 15055 0.05% 78 16212 0.05% 79 17960 0.06% 80 19299 0.06% 81 21447 0.07% 82 23963 0.08% 83 26038 0.09% 84 28829 0.10% 85 30896 0.10% 86 32112 0.11% 87 33994 0.11% 88 35921 0.12% 89 38169 0.13% 90 40982 0.14% 91 42460 0.14% 92 45108 0.15% 93 47959 0.16% 94 50884 0.17% 95 53335 0.18% 96 55941 0.19% 97 57521 0.19% 98 58847 0.19% 99 61690 0.20% 100 63294 0.21% 101 64312 0.21% 102 66994 0.22% 103 69341 0.23% 104 70834 0.23% 105 73659 0.24% 106 74729 0.25% 107 76579 0.25% 108 78185 0.26% 109 79801 0.26% 110 80445 0.27% 111 81419 0.27% 112 83086 0.28% 113 84259 0.28% 114 87396 0.29% 115 88573 0.29% 116 90630 0.30% 117 91990 0.30% 118 92017 0.30% 119 93278 0.31% 120 94645 0.31% 121 96356 0.32% 122 95066 0.31% 123 98270 0.33% 124 100045 0.33% 125 101141 0.33% 126 102329 0.34% 127 101237 0.34% 128 101196 0.34% 129 103206 0.34% 130 102499 0.34% 131 102871 0.34% 132 105408 0.35% 133 104812 0.35% 134 105361 0.35% 135 105683 0.35% 136 108456 0.36% 137 107167 0.35% 138 108109 0.36% 139 109858 0.36% 140 109634 0.36% 141 109188 0.36% 142 111371 0.37% 143 110771 0.37% 144 112016 0.37% 145 114984 0.38% 146 113177 0.37% 147 115242 0.38% 148 113482 0.38% 149 114641 0.38% 150 114597 0.38% 151 24328147 80.56% 30199247 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=4.08 fanout-score-rank=26 prefix-density=0.32 prefix-fanout=2.9 sequence=TCCTGGATCTTGGCCTTCACGTTGTCGATGGTGTC criterion=fanout-score sequence-density=0.04 sequence-density-rank=39 fanout-score=279.18 fanout-score-rank=1 prefix-density=0.58 prefix-fanout=18.3 sequence=GGCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAG criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=1.60 fanout-score-rank=41 prefix-density=0.11 prefix-fanout=1.6 sequence=ACACACACACAGGTGTGTAAGCCAGCTCAGTAGCTAGCTCCTCGAGCTTTTGATCAACCGGCGGCCATGGCGGAGGAAAAGAAGCACCACCTGTTCCACCACAAGAAGGAAGACACCGACGTGGTCGTCAACCCCACCACGGGCGCCGTCGACGAGTACGGGTACTCGGCGGAGACGGTGGTAACCCCAACCGGCGCCGACGGCGAGTACGAGCGCATCACC criterion=fanout-score sequence-density=0.13 sequence-density-rank=26 fanout-score=134.71 fanout-score-rank=1 prefix-density=0.85 prefix-fanout=21.0 sequence=CGCCGCCGCCGTC SRR13165373 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:39:36 Started mapping on | Dec 07 16:39:36 Finished on | Dec 07 16:42:21 Mapping speed, Million of reads per hour | 658.89 Number of input reads | 30199247 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 28384774 Uniquely mapped reads % | 93.99% Average mapped length | 288.91 Number of splices: Total | 26328659 Number of splices: Annotated (sjdb) | 24561894 Number of splices: GT/AG | 26015767 Number of splices: GC/AG | 258193 Number of splices: AT/AC | 14053 Number of splices: Non-canonical | 40646 Mismatch rate per base, % | 0.23% Deletion rate per base | 0.02% Deletion average length | 3.35 Insertion rate per base | 0.01% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 257215 % of reads mapped to multiple loci | 0.85% Number of reads mapped to too many loci | 98323 % of reads mapped to too many loci | 0.33% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.49% % of reads unmapped: other | 1.34% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1557579 1557579 1557579 N_multimapping 257215 257215 257215 N_noFeature 926853 27654020 1169310 N_ambiguous 555778 3607 69053 UnstrandedReadsAssigned:26902143 PositiveStrandReadsAssigned:727147 NegativeStrandReadsAssigned:27146411 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165373 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165373-trimmed-pair1.fastq SRR13165373-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 30,199,247 reads, 27,615,047 reads pseudoaligned [quant] estimated average fragment length: 247.566 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,188 rounds 52973 SRR13165373.ke.tsv 35125 SRR13165373.se.tsv 88098 total ==> SRR13165373.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 690.166 0 0 PNS24247 1044 797.434 50.6955 3.55286 PNS24249 1928 1681.43 96.1326 3.19517 PNS24246 1044 797.434 50.6955 3.55286 PNS24248 1044 797.434 50.6955 3.55286 PNS24244 1471 1224.43 158.781 7.24714 PNS24243 293 112.53 0 0 KQK14069 1603 1356.43 32 1.31842 KQK14071 474 252.307 0 0 ==> SRR13165373.se.tsv <== BRADI_1g14170v3 31 BRADI_1g53295v3 239 BRADI_1g59795v3 440 BRADI_1g07683v3 0 BRADI_1g00485v3 14 BRADI_1g20270v3 768 BRADI_1g74790v3 611 BRADI_1g09890v3 5 BRADI_1g77505v3 306 BRADI_1g48960v3 0 SRR13165373 completed mapping pipeline successfully