Starting /dee2/code/volunteer_pipeline.sh SRR13165375 current disk space = 1541703266304 free memory = 1415051076 SRR13165375 SRAfilesize 751dc98ad937564fbc8e637bfef41279 SRR13165375.sra SRR13165375.sra file validated SRR13165375 is paired end SRR13165375 is conventional basespace SRR13165375 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165375_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4805 37.0 37.0 37.0 37.0 37.0 2 36.005 37.0 37.0 37.0 37.0 37.0 3 36.415 37.0 37.0 37.0 37.0 37.0 4 36.478 37.0 37.0 37.0 37.0 37.0 5 36.5345 37.0 37.0 37.0 37.0 37.0 6 36.5835 37.0 37.0 37.0 37.0 37.0 7 36.3495 37.0 37.0 37.0 37.0 37.0 8 36.5655 37.0 37.0 37.0 37.0 37.0 9 36.508 37.0 37.0 37.0 37.0 37.0 10-14 36.542199999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.5056 37.0 37.0 37.0 37.0 37.0 20-24 36.4975 37.0 37.0 37.0 37.0 37.0 25-29 36.42210000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.393899999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.3212 37.0 37.0 37.0 37.0 37.0 40-44 36.388 37.0 37.0 37.0 37.0 37.0 45-49 36.335 37.0 37.0 37.0 37.0 37.0 50-54 36.39489999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.279199999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.2618 37.0 37.0 37.0 37.0 37.0 65-69 36.275999999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.253 37.0 37.0 37.0 37.0 37.0 75-79 36.278800000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.116499999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.1796 37.0 37.0 37.0 37.0 37.0 90-94 36.153200000000005 37.0 37.0 37.0 37.0 37.0 95-99 36.1049 37.0 37.0 37.0 37.0 37.0 100-104 36.12179999999999 37.0 37.0 37.0 37.0 37.0 105-109 36.1554 37.0 37.0 37.0 37.0 37.0 110-114 36.1052 37.0 37.0 37.0 37.0 37.0 115-119 36.0594 37.0 37.0 37.0 37.0 37.0 120-124 36.08 37.0 37.0 37.0 37.0 37.0 125-129 35.927499999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.9501 37.0 37.0 37.0 37.0 37.0 135-139 35.882400000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.730000000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.4892 37.0 37.0 37.0 37.0 37.0 150-151 35.32875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 1.0 25 1.0 26 7.0 27 12.0 28 18.0 29 23.0 30 27.0 31 35.0 32 50.0 33 92.0 34 137.0 35 331.0 36 2848.0 37 416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.65 10.375 5.3 32.675 2 23.713420787083752 9.889001009081735 33.4510595358224 32.94651866801211 3 22.3 14.124999999999998 24.875 38.7 4 28.925 21.925 21.25 27.900000000000002 5 28.799999999999997 26.1 22.55 22.55 6 24.725 30.875000000000004 20.674999999999997 23.724999999999998 7 20.775 26.174999999999997 36.15 16.900000000000002 8 21.575 24.4 29.325000000000003 24.7 9 22.85 19.875 32.95 24.325 10-14 24.25 26.35 25.105 24.295 15-19 24.575 24.435000000000002 24.87 26.119999999999997 20-24 24.79 24.735 25.080000000000002 25.395 25-29 24.55 24.58 24.97 25.900000000000002 30-34 24.675 23.77 24.345 27.21 35-39 24.6 23.47 25.91 26.02 40-44 25.174999999999997 23.835 24.95 26.040000000000003 45-49 24.48 23.455000000000002 24.345 27.72 50-54 23.735 24.68 24.86 26.724999999999998 55-59 24.57 24.175 24.285 26.97 60-64 24.385 23.56 25.665 26.39 65-69 25.019999999999996 24.245 24.69 26.045 70-74 24.775 23.880000000000003 24.315 27.029999999999998 75-79 25.465 23.799999999999997 24.3 26.435 80-84 24.75 24.145 24.834999999999997 26.27 85-89 25.085 24.205 24.805 25.905 90-94 25.525 24.64 23.855 25.979999999999997 95-99 25.395 24.93 23.765 25.91 100-104 25.585 24.4 23.51 26.505000000000003 105-109 25.71 25.124999999999996 23.62 25.545 110-114 25.365 25.124999999999996 23.62 25.89 115-119 25.119999999999997 24.585 23.935000000000002 26.36 120-124 26.305 24.905 22.814999999999998 25.974999999999998 125-129 25.045 24.87 23.36 26.724999999999998 130-134 24.22 25.345000000000002 23.24 27.195000000000004 135-139 24.645 24.33 23.845 27.18 140-144 24.565 25.025 23.06 27.35 145-149 24.404999999999998 25.34 23.435 26.82 150-151 24.325 24.087500000000002 23.775 27.8125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 1.5 26 2.0 27 2.5 28 4.5 29 5.5 30 6.0 31 6.5 32 7.5 33 10.5 34 17.0 35 28.5 36 44.0 37 52.0 38 67.0 39 85.0 40 91.0 41 111.5 42 143.0 43 148.5 44 169.5 45 181.5 46 172.5 47 173.5 48 160.0 49 169.0 50 159.5 51 138.0 52 136.5 53 129.0 54 121.5 55 127.5 56 120.5 57 111.5 58 114.5 59 102.5 60 87.5 61 72.5 62 71.5 63 72.5 64 64.0 65 63.0 66 61.0 67 51.5 68 54.5 69 56.5 70 39.0 71 28.5 72 28.0 73 25.5 74 28.5 75 24.0 76 14.5 77 8.5 78 7.5 79 8.0 80 5.5 81 3.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.8999999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.05 #Duplication Level Percentage of deduplicated Percentage of total 1 69.68680089485458 46.725 2 19.351230425055927 25.95 3 6.599552572706935 13.275 4 2.49813571961223 6.7 5 0.9321401938851605 3.125 6 0.5965697240865026 2.4 7 0.11185682326621924 0.525 8 0.14914243102162564 0.8 9 0.03728560775540641 0.22499999999999998 >10 0.03728560775540641 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTACATGTTGCAGCCGTTGCAGCCGCTGCCGCACTTGCAGGATGACCCG 11 0.27499999999999997 No Hit CCGCCATCTCAACCTCTGTCTCTAGAACTTTCTCGCGCGACTTGGGAGTT 9 0.22499999999999998 No Hit CCCTGAGCAAATTCGACGTACGAACAGGATATATATGAATTCCTTTTAAG 8 0.2 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 8 0.2 No Hit ACTTGCTCCGTGTCTCGTGCTACCCCTCCTGCTGCTTCTGGTTGTGAAGA 8 0.2 No Hit GCCCTTTGCATGCATACGTTCAATAATTTTGAGGGAGCAGCACGTAAAAC 8 0.2 No Hit GCAGTGTAATTAAATTTGGGATAATATAATCCTTACAAGTCCAACATTAG 7 0.17500000000000002 No Hit TCTTCTTGTAATTTTCAGTGGATATTGCATCATCGAGGGACTCGGGGCTA 7 0.17500000000000002 No Hit GTCAGGGATGTACACGTCGTCGGGGACCTCCATCTCCACCTCCCCCTCCG 7 0.17500000000000002 No Hit AAGAAAAGTGCGATCTGAATAACCAGAAACTTTTGTGGCCAAAATTACCT 6 0.15 No Hit GGGATGCAGGGTCTGGCGTGCGATTGGGTAGCCGTTGAACGGGAAGCGCA 6 0.15 No Hit GTCGTGAAGTTGGTAGAGAAGGCTGGCCGGCGGGCCAGGGTGGCGCCGGC 6 0.15 No Hit CTCCTCCGGCGCCGGCACGTTGGCTGATGCGCCGCGCGGCACCGCCGGGG 6 0.15 No Hit GAGGGAAATAAATGTCAAAGAAAAAGAGCCCATCATGGTAAGGAGTGCCT 6 0.15 No Hit CCCTAGCTAATATTGCCTCCATATGGGATTATGGGAGAGCAAAATCCAGA 6 0.15 No Hit GTTCGCTTGATCTGCAGTATCGGAGGGCAGAGCCCTGCATCGGTCATGCA 6 0.15 No Hit CCATGCACCAATATCTCTGAAGAAGGAAATGGTTGCAGGAGTAACAGTAC 6 0.15 No Hit CCTGCATACACCGATACGGTCTTCGCGGGTGGGGGCCCAGTAGAACTTCT 6 0.15 No Hit CTTCACGTTGTCGATGGTGTCGGAGCTCTCGACCTCCAAGGTGATGGTCT 6 0.15 No Hit TGTCTTTGCTGTTTCCAATTTTAAATATTGCAGCCACCCCTTCGTACTGA 6 0.15 No Hit AGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCC 6 0.15 No Hit GGTGTGTACAAGGCCCGGGAACGGATTCACCGCCGTATGGCTGACCGGCG 6 0.15 No Hit CTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCATTGCGGAAAATTCCC 6 0.15 No Hit CATGAATTGATGTAAGCTTGCTCTTTGAATTTGAAGCCTCTCCATTTGGA 6 0.15 No Hit CCAGTTAATTGTATCATCTGTATGGTCGTACCTGGAATAGCGGAATCGAA 6 0.15 No Hit CTCAACATTGAACCCGATGGTTGGGATGGTGGTGACGATCTCGCCGAGCT 5 0.125 No Hit AGAAATTTGAATGATGCGTCGCCGGCACGAGGGCCGTGCGATCCGTCGAG 5 0.125 No Hit TTCATCGATCCTCCTGACACCGGCCTGCAAATGCCACTCGTTTCCCCTCC 5 0.125 No Hit ACCGCGTCCTCCATCTCCGGCCGGCGGCCGCAAATTGAAGTGAACCCCCA 5 0.125 No Hit CTGGTTGTTGTCGTTGCTGTTCATCATGCCGCCGCCCTGGCTCGCGGCGT 5 0.125 No Hit GCTTCAAGTGCACTCTGGAATTTGTATGGCGCTTCTCCAAGCACCTGGGC 5 0.125 No Hit GTCTGCTAAGAAAGTCACGTACAGAAGTGGGAGGTAAAGAGAATGGTAAC 5 0.125 No Hit GTAGACACTATGCTCTCCTCATCTAAGCATCTTAGAGTTAAACGGTAGCC 5 0.125 No Hit GCCGAGTTTTCGAGTTATATACCAGCACGGTTTTGCAATTTGCACACTTG 5 0.125 No Hit GTTGGACCAGATAGAAACTTTGGAGAGGAAGGGCCAGTTGAATATGCTAG 5 0.125 No Hit GATGGGGTGGTCTGGCGACGCGCGACGGCGAGACCCGGAAGATTCTCAAA 5 0.125 No Hit TGTATGAAGTGGCATCTCCATGTCTGGAACTTCCCTTAGAGGCCTGTCAG 5 0.125 No Hit GTCGTTCGAGCTTTTCCTGGGAGTATGGCATCGGTTACATACTTCAGTGC 5 0.125 No Hit GGGTACATGTTGCAGCCGTTGCAGCCGCTGCCGCACTTGCAGGATGACCC 5 0.125 No Hit GTCGCCGAGCCCCCGGTCACCGTCGCCCTCGCCTTCTTCGTCGTGCCAAC 5 0.125 No Hit GTATCGAGTAGTTGGATCATAGTCTCTTACCATAAAAATTGCTGCATGTG 5 0.125 No Hit GTCACAATTGACAAAATGAATCGACAAACATACTGGGCTGCAATTCCAGT 5 0.125 No Hit GGATCGAGTTCGATACTTCGAATTCTCATTACAAAGAATCGATCGTCCGA 5 0.125 No Hit GCCCACTGAGATATGTCTAGCCAATTATCATTCCTTGGGACTTACTGACC 5 0.125 No Hit ATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATA 5 0.125 No Hit GCCGCAGGCTCCACGCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 5 0.125 No Hit GCTGTTGCTGCTGAAACAATAGAGAGGCTCGTAACTGATTCGACTAAGCC 5 0.125 No Hit CCTACATCCAGGCTTATTTTGGTTCATGCTACAACATTACAGATTACAGA 5 0.125 No Hit GTTCCCCATCAAGCCTAGAAAGCCCAAACGAGGTTGCAGACAAGAAACCT 5 0.125 No Hit GCGTAGCATTCTCGTCCAGGAAAGGTTCCACGGCGTTGGGGTTTCCCCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.3 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.4375 0.0 0.0 0.0 0.0 82-83 0.7 0.0 0.0 0.0 0.0 84-85 1.0125 0.0 0.0 0.0 0.0 86-87 1.375 0.0 0.0 0.0 0.0 88-89 1.85 0.0 0.0 0.0 0.0 90-91 2.4125 0.0 0.0 0.0 0.0 92-93 2.7375 0.0 0.0 0.0 0.0 94-95 3.075 0.0 0.0 0.0 0.0 96-97 3.75 0.0 0.0 0.0 0.0 98-99 4.375 0.0 0.0 0.0 0.0 100-101 4.975 0.0 0.0 0.0 0.0 102-103 5.4375 0.0 0.0 0.0 0.0 104-105 6.35 0.0 0.0 0.0 0.0 106-107 6.875 0.0 0.0 0.0 0.0 108-109 7.5875 0.0 0.0 0.0 0.0 110-111 8.6875 0.0 0.0 0.0 0.0 112-113 9.525 0.0 0.0 0.0 0.0 114-115 10.2375 0.0 0.0 0.0 0.0 116-117 11.100000000000001 0.0 0.0 0.0 0.0 118-119 12.0625 0.0 0.0 0.0 0.0 120-121 12.825 0.0 0.0 0.0 0.0 122-123 13.575 0.0 0.0 0.0 0.0 124-125 14.6 0.0 0.0 0.0 0.0 126-127 15.524999999999999 0.0 0.0 0.0 0.0 128-129 16.1 0.0 0.0 0.0 0.0 130-131 16.9625 0.0 0.0 0.0 0.0 132-133 18.0875 0.0 0.0 0.0 0.0 134-135 18.7125 0.0 0.0 0.0 0.0 136-137 19.7125 0.0 0.0 0.0 0.0 138-139 20.7875 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTTGA 10 0.006830828 145.0 4 GAGGACT 10 0.006830828 145.0 6 AGGACTT 10 0.006830828 145.0 7 GGACTTA 10 0.006830828 145.0 8 TCGGAGG 10 0.006830828 145.0 3 TAGTTAA 10 0.006830828 145.0 145 GGAGGAC 10 0.006830828 145.0 5 GACTTAA 10 0.006830828 145.0 9 CATGCAT 10 0.006830828 145.0 1 GGGGGGG 35 0.0035366106 41.428574 145 >>END_MODULE SRR13165375 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165375_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.11825 37.0 37.0 37.0 37.0 37.0 2 36.332 37.0 37.0 37.0 37.0 37.0 3 36.248 37.0 37.0 37.0 37.0 37.0 4 36.34 37.0 37.0 37.0 37.0 37.0 5 36.317 37.0 37.0 37.0 37.0 37.0 6 36.353 37.0 37.0 37.0 37.0 37.0 7 36.305 37.0 37.0 37.0 37.0 37.0 8 36.3375 37.0 37.0 37.0 37.0 37.0 9 36.341 37.0 37.0 37.0 37.0 37.0 10-14 36.3892 37.0 37.0 37.0 37.0 37.0 15-19 36.3022 37.0 37.0 37.0 37.0 37.0 20-24 36.2884 37.0 37.0 37.0 37.0 37.0 25-29 36.229400000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.209799999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.189099999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.1937 37.0 37.0 37.0 37.0 37.0 45-49 36.13295000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.1541 37.0 37.0 37.0 37.0 37.0 55-59 36.1687 37.0 37.0 37.0 37.0 37.0 60-64 36.0837 37.0 37.0 37.0 37.0 37.0 65-69 36.0894 37.0 37.0 37.0 37.0 37.0 70-74 36.0638 37.0 37.0 37.0 37.0 37.0 75-79 36.06225 37.0 37.0 37.0 37.0 37.0 80-84 36.038850000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.9707 37.0 37.0 37.0 37.0 37.0 90-94 35.90685 37.0 37.0 37.0 37.0 37.0 95-99 35.8823 37.0 37.0 37.0 37.0 37.0 100-104 35.9072 37.0 37.0 37.0 37.0 37.0 105-109 35.9534 37.0 37.0 37.0 37.0 37.0 110-114 35.77470000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.760400000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.630700000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.63195 37.0 37.0 37.0 37.0 37.0 130-134 35.4719 37.0 37.0 37.0 37.0 37.0 135-139 35.33005000000001 37.0 37.0 37.0 37.0 37.0 140-144 35.1625 37.0 37.0 37.0 29.8 37.0 145-149 34.900549999999996 37.0 37.0 37.0 25.0 37.0 150-151 34.6295 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 1.0 15 1.0 16 1.0 17 1.0 18 1.0 19 0.0 20 3.0 21 1.0 22 2.0 23 4.0 24 8.0 25 4.0 26 6.0 27 12.0 28 10.0 29 16.0 30 31.0 31 37.0 32 63.0 33 96.0 34 222.0 35 569.0 36 2634.0 37 274.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.285355438332076 19.643305702084906 8.666164280331575 27.405174579251444 2 28.799999999999997 22.625 27.325 21.25 3 23.5 23.275000000000002 29.65 23.575 4 27.700000000000003 29.825000000000003 19.45 23.025000000000002 5 27.6 33.225 19.225 19.950000000000003 6 22.85 36.825 18.325 22.0 7 23.95 19.1 32.225 24.725 8 24.325 22.475 23.150000000000002 30.049999999999997 9 24.3 21.099999999999998 25.2 29.4 10-14 25.795 26.1 22.25 25.855 15-19 25.929999999999996 25.615 23.215 25.240000000000002 20-24 24.702410723216964 25.672701810543163 23.15694708412524 26.467940382114634 25-29 26.123061530765384 24.99749874937469 23.1815907953977 25.697848924462228 30-34 25.740296118447382 24.754901960784316 23.739495798319325 25.765306122448976 35-39 26.14784435330599 25.137541262378715 23.39701910573172 25.31759527858358 40-44 26.665666266506605 24.439775910364144 23.169267707082835 25.725290116046416 45-49 27.01445505927074 24.698644525583955 23.103086080128044 25.183814335017257 50-54 26.582974892467742 24.582374712413724 23.642092627788337 25.192557767330197 55-59 26.43821910955478 24.262131065532767 23.70185092546273 25.597798899449725 60-64 26.20786235870761 23.867160148044412 23.687106131839553 26.23787136140842 65-69 26.47029405881176 24.20984196839368 23.869773954790958 25.4500900180036 70-74 26.028014007003502 24.8224112056028 23.326663331665834 25.822911455727866 75-79 26.161772797758992 24.85618528337752 23.360512230503726 25.62152968835976 80-84 26.576644161040257 24.63115778944736 23.520880220055012 25.271317829457363 85-89 27.483741870935468 24.482241120560282 23.261630815407706 24.772386193096548 90-94 27.8297404091432 24.55859550842795 23.14310008502976 24.46856399739909 95-99 27.608282484745423 25.17255176552966 22.7518255476643 24.46734020206062 100-104 28.789394697348676 23.866933466733368 22.651325662831415 24.692346173086545 105-109 27.443721860930463 24.54727363681841 24.09704852426213 23.911955977988995 110-114 28.023407022106632 25.8777633289987 22.131639491847555 23.967190157047114 115-119 28.65432716358179 24.987493746873437 22.256128064032016 24.102051025512754 120-124 28.829414707353678 24.742371185592795 22.521260630315158 23.90695347673837 125-129 27.997598919513784 25.24135861137512 23.405532489620327 23.355509979490773 130-134 30.138082849709825 24.474684810886533 22.798679207524515 22.588553131879127 135-139 30.506778728300567 24.64355395467507 22.442343288808843 22.40732402821552 140-144 32.16965089526858 23.987196158847652 22.206661998599582 21.636490947284184 145-149 32.913102206213416 24.393416379008453 20.65135824703587 22.042123167742258 150-151 34.50087565674256 23.805354015511636 21.128346259694773 20.565424068051037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.5 12 1.0 13 0.5 14 0.5 15 0.5 16 0.0 17 0.0 18 1.0 19 1.0 20 0.0 21 0.0 22 0.0 23 0.5 24 2.5 25 2.0 26 0.0 27 1.5 28 2.0 29 2.0 30 6.0 31 9.5 32 17.0 33 21.5 34 22.0 35 23.5 36 37.0 37 47.0 38 60.5 39 76.0 40 79.0 41 93.5 42 112.5 43 139.5 44 168.0 45 173.5 46 182.0 47 176.5 48 162.5 49 162.0 50 142.0 51 136.5 52 135.5 53 138.5 54 140.0 55 126.5 56 112.5 57 106.0 58 100.5 59 94.5 60 92.5 61 75.5 62 72.0 63 80.0 64 84.5 65 69.0 66 65.0 67 72.5 68 64.0 69 54.0 70 40.5 71 40.5 72 35.0 73 29.5 74 26.5 75 18.5 76 13.5 77 10.0 78 8.0 79 7.0 80 5.5 81 2.5 82 3.0 83 3.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.475 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.04 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.02 70-74 0.05 75-79 0.045 80-84 0.025 85-89 0.05 90-94 0.034999999999999996 95-99 0.03 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.06 135-139 0.055 140-144 0.03 145-149 0.055 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8 #Duplication Level Percentage of deduplicated Percentage of total 1 69.49850299401199 46.425 2 19.311377245508982 25.8 3 7.0359281437125745 14.099999999999998 4 2.282934131736527 6.1 5 0.93562874251497 3.125 6 0.561377245508982 2.25 7 0.18712574850299402 0.8750000000000001 8 0.07485029940119761 0.4 9 0.07485029940119761 0.44999999999999996 >10 0.037425149700598806 0.475 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 19 0.475 No Hit CATTTCACACGCCTACACAGATCGGAGGAACCGAGGACCCAACTCCCAAG 9 0.22499999999999998 No Hit AACCGGAGCAAAAGTTGGGCAGCAAACATGGCAATCTGCAGCGCACACAC 9 0.22499999999999998 No Hit AAGCATTAGAATGGAGACTCAAACATGCCAAACATTCCATTGAAGACTTG 8 0.2 No Hit CGGACGCGGTGGCGACGGCGAACGTGCTGGAGAGCGCGGCGCCGGTGGTG 8 0.2 No Hit GAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAA 7 0.17500000000000002 No Hit CACACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAG 7 0.17500000000000002 No Hit CACACAAGCAAGCAAGCAAGCTCTCAGCTCTCAGCAGCAATGGCGACCGC 7 0.17500000000000002 No Hit TGACTCTTTGGTTGCTAGTGCAGAGAACTCAGTTGTTGATCATGAAGTAA 7 0.17500000000000002 No Hit GCCCGGGGCAAGGCTCATCACCCAGTGCGTCACGCCTGACAGAGTAGCTG 7 0.17500000000000002 No Hit GTGAAGGCCAAGATCCAGGACAAGGAGGGCATTCCCCCGGACCAGCAGCG 6 0.15 No Hit CCGGAAGCGCCCATGGGGACGTTACGCCGCGGAGATCCGCGACCCGGCTA 6 0.15 No Hit AATCTCCTTTCCCCTCTCCGCCTCTCACCGTCGCGTCGCCCTGCCGTATT 6 0.15 No Hit ATTGCAGCCGCGAGCAGAACAACAGCTAGCTCACATGGCCGCCGCCGCCT 6 0.15 No Hit CCGGCGGGCGCGACCGAGACGCAGGTTCGCGAAGAAAAGTCTGGTGGCGT 6 0.15 No Hit GGGGAATCTTACCTTGATGTGATTCAGAGGTTGGAGCCTGTCATTATCGA 6 0.15 No Hit TATTGACTCACTTAATAAGTGCAAGACCTAAGCTTCAGAATGGCATGGTT 6 0.15 No Hit AAAGACTGATAACGAGATCACCGGCACTCCGGCAGGGTTGCGTCCACGAG 6 0.15 No Hit GCTCAACCGGAATAGAGAACATCGGCAAGAAGCTGGTGAACTCGAAGGAT 6 0.15 No Hit GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCAC 6 0.15 No Hit GTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTGCTAGGCGA 6 0.15 No Hit GACTCAGGGAAGGAGTGATTTGTATATAGGCAGGTGGATGCAATCCAAGC 6 0.15 No Hit ATATCCCTGTACTGCCTTGCCAGAGCCATCGAGAGTTTCTTCACATGCAT 6 0.15 No Hit GGAAAGTCTTAGAAAAAGACTTGCCAGATACCATATTTGTGAGGGCATAT 6 0.15 No Hit TGTAGATCCTAATGGTGACATCAAACTTGCGGATTTTGGTATGGCCAAGC 6 0.15 No Hit CAGGTATCAACAAGTTCTTCACCGACCCAAAGTGAAGGGTTTGGATTCGG 5 0.125 No Hit AGTTACTGCTGATGTCTTGCTCAAGACTATATATGTCTTTGGCTTTGGTG 5 0.125 No Hit CATGAAGATCTTTGGTTTTCTAATGCGAGCTATGACAGCTGCTGTTTGCA 5 0.125 No Hit GTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGA 5 0.125 No Hit GCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTTAAGGCCA 5 0.125 No Hit GGCTCTTCGCCAAGAAGGAGATGCGGATCCTCATGGTCGGTCTCGACGCC 5 0.125 No Hit ATAGCAAACTCCATATAGTTATTGGTAACCTTTTAACTAAAGCTAATATA 5 0.125 No Hit AGAGGATGAGGCCAAGTCATCATGCCCCTTATGCCCTGGGCGACACACGT 5 0.125 No Hit GTCACTGCATCGGGTGGCAAGTCTGGAGCATCTCCAGAAGAGGATGTGCG 5 0.125 No Hit GCCGTGTTTAGGTAGCCTAAACAAACAATGATACTCCCTCCGTTTCTAAA 5 0.125 No Hit GGAAGGTAGAGTTTTCCTCAAATCATCAATGAACATGTACTCATTTTCTC 5 0.125 No Hit CCCAGCTCCTTCCTGGGCCAGCCCAGCAACAACAAGCCGTTCCACGGCCT 5 0.125 No Hit CTCATAGCACAGTCGGTAACGAATGCGATAATAAAGGACGTATTAATCCT 5 0.125 No Hit GGGGGGTGCTGTAGTGTTCGAGGACTCGGCGGCGTCTGCAGCCACGGTCG 5 0.125 No Hit TGATAAACCCCGAGAGGCTGGCCATGATGAAGAAGGAGGCGGTGCTGGTG 5 0.125 No Hit GGTGAAGATCGTGATGTAGATGCAGAAAGTAATTGGACTGCTCGAACTCC 5 0.125 No Hit CTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTTGAACTGTGA 5 0.125 No Hit AGAGACAATAGAGAATGATCTTTCTCAGCTTCGTGAGAAGGGTGTTGATG 5 0.125 No Hit GAGCTTCTCATGGGTCAAAGACAAAGATTGCAGAAGTGATGACCTCCCCG 5 0.125 No Hit CAAAGATACAATGGCGTGCTCTTGATGAGATAAATTCTGGGGTATGTGAC 5 0.125 No Hit GTTGTAGCTCATCATATGTACTCTATGCCCCCCTATCCATACCTAGCTAC 5 0.125 No Hit GTCCGTGGTGGACCTGGTGGTGCAGGTCTCCAAGAAGACCCTGGCCGAGG 5 0.125 No Hit GGGTAAGAAGGGGTAGAGAAAATGCCTCGAGCCGAGGTCCGAGTACCAAG 5 0.125 No Hit ACCCCTTCCTCTACGTGCAGGTGAGGCAGGCAGCTGCTTTCTTTGGCCCA 5 0.125 No Hit CAACCACAATTACAACTACAACGGCAACAGAAAGCACGTCGTCTACAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.2375 0.0 0.0 0.0 0.0 76-77 0.325 0.0 0.0 0.0 0.0 78-79 0.375 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.725 0.0 0.0 0.0 0.0 84-85 1.0375 0.0 0.0 0.0 0.0 86-87 1.4 0.0 0.0 0.0 0.0 88-89 1.875 0.0 0.0 0.0 0.0 90-91 2.4625 0.0 0.0 0.0 0.0 92-93 2.8125 0.0 0.0 0.0 0.0 94-95 3.15 0.0 0.0 0.0 0.0 96-97 3.825 0.0 0.0 0.0 0.0 98-99 4.425000000000001 0.0 0.0 0.0 0.0 100-101 5.05 0.0 0.0 0.0 0.0 102-103 5.5375 0.0 0.0 0.0 0.0 104-105 6.449999999999999 0.0 0.0 0.0 0.0 106-107 6.987500000000001 0.0 0.0 0.0 0.0 108-109 7.7125 0.0 0.0 0.0 0.0 110-111 8.850000000000001 0.0 0.0 0.0 0.0 112-113 9.7 0.0 0.0 0.0 0.0 114-115 10.4 0.0 0.0 0.0 0.0 116-117 11.25 0.0 0.0 0.0 0.0 118-119 12.225 0.0 0.0 0.0 0.0 120-121 12.912500000000001 0.0 0.0 0.0 0.0 122-123 13.6875 0.0 0.0 0.0 0.0 124-125 14.7375 0.0 0.0 0.0 0.0 126-127 15.625 0.0 0.0 0.0 0.0 128-129 16.175 0.0 0.0 0.0 0.0 130-131 17.025 0.0 0.0 0.0 0.0 132-133 18.112499999999997 0.0 0.0 0.0 0.0 134-135 18.7125 0.0 0.0 0.0 0.0 136-137 19.7125 0.0 0.0 0.0 0.0 138-139 20.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACTCC 10 0.006830828 145.0 6 CTCCATA 10 0.006830828 145.0 9 CAACAAG 10 0.006830828 145.0 1 TAGCAAA 10 0.006830828 145.0 2 TTGGGTC 10 0.006830828 145.0 7 ATAGCAA 10 0.006830828 145.0 1 CTGCATT 10 0.006830828 145.0 145 CAAACTC 10 0.006830828 145.0 5 GGGGGGG 420 1.1641532E-10 10.357142 145 >>END_MODULE Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573453 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573453 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra Read 1573438 spots for SRR13165375.sra Written 1573438 spots for SRR13165375.sra SRR ids: ['SRR13165375.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_pzattka2 SRR13165375.sra spots: 31468775 blocks: [[1, 1573438], [1573439, 3146876], [3146877, 4720314], [4720315, 6293752], [6293753, 7867190], [7867191, 9440628], [9440629, 11014066], [11014067, 12587504], [12587505, 14160942], [14160943, 15734380], [15734381, 17307818], [17307819, 18881256], [18881257, 20454694], [20454695, 22028132], [22028133, 23601570], [23601571, 25175008], [25175009, 26748446], [26748447, 28321884], [28321885, 29895322], [29895323, 31468775]] SRR13165375 file size 10672766 SRR13165375 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165375 SRR13165375_1.fastq SRR13165375_2.fastq Input file: SRR13165375_1.fastq Paired file: SRR13165375_2.fastq trimmed: SRR13165375-trimmed-pair1.fastq, SRR13165375-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:50:35 2024 >> started Sat Dec 7 16:54:35 2024 >> done (239.732s) 31468775 read pairs processed; of these: 804 ( 0.00%) short read pairs filtered out after trimming by size control 23022 ( 0.07%) empty read pairs filtered out after trimming by size control 31444949 (99.92%) read pairs available; of these: 7781811 (24.75%) trimmed read pairs available after processing 23663138 (75.25%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 51 0.00% 19 40 0.00% 20 63 0.00% 21 48 0.00% 22 56 0.00% 23 46 0.00% 24 45 0.00% 25 76 0.00% 26 96 0.00% 27 79 0.00% 28 102 0.00% 29 115 0.00% 30 116 0.00% 31 145 0.00% 32 114 0.00% 33 167 0.00% 34 165 0.00% 35 182 0.00% 36 177 0.00% 37 216 0.00% 38 278 0.00% 39 307 0.00% 40 328 0.00% 41 392 0.00% 42 390 0.00% 43 451 0.00% 44 450 0.00% 45 505 0.00% 46 540 0.00% 47 652 0.00% 48 760 0.00% 49 931 0.00% 50 1094 0.00% 51 1220 0.00% 52 1300 0.00% 53 1389 0.00% 54 1679 0.01% 55 1779 0.01% 56 1914 0.01% 57 2343 0.01% 58 2539 0.01% 59 2790 0.01% 60 3297 0.01% 61 3913 0.01% 62 4344 0.01% 63 4980 0.02% 64 5670 0.02% 65 6107 0.02% 66 6504 0.02% 67 7175 0.02% 68 8041 0.03% 69 9067 0.03% 70 10305 0.03% 71 11763 0.04% 72 13236 0.04% 73 14972 0.05% 74 16326 0.05% 75 18274 0.06% 76 19737 0.06% 77 21615 0.07% 78 23062 0.07% 79 26065 0.08% 80 28043 0.09% 81 30685 0.10% 82 33849 0.11% 83 37322 0.12% 84 41456 0.13% 85 44429 0.14% 86 47983 0.15% 87 50137 0.16% 88 52628 0.17% 89 55472 0.18% 90 58667 0.19% 91 61914 0.20% 92 64599 0.21% 93 68847 0.22% 94 72797 0.23% 95 76959 0.24% 96 79563 0.25% 97 84396 0.27% 98 85262 0.27% 99 88438 0.28% 100 90714 0.29% 101 92736 0.29% 102 95595 0.30% 103 97579 0.31% 104 99621 0.32% 105 101282 0.32% 106 105186 0.33% 107 107106 0.34% 108 108677 0.35% 109 111451 0.35% 110 111463 0.35% 111 113384 0.36% 112 115442 0.37% 113 114246 0.36% 114 118093 0.38% 115 120121 0.38% 116 121490 0.39% 117 122946 0.39% 118 123689 0.39% 119 124876 0.40% 120 129208 0.41% 121 127274 0.40% 122 126702 0.40% 123 130349 0.41% 124 130271 0.41% 125 131200 0.42% 126 131465 0.42% 127 133445 0.42% 128 132516 0.42% 129 134660 0.43% 130 132308 0.42% 131 133216 0.42% 132 134356 0.43% 133 135596 0.43% 134 132728 0.42% 135 134585 0.43% 136 134040 0.43% 137 132064 0.42% 138 131027 0.42% 139 135013 0.43% 140 136002 0.43% 141 136349 0.43% 142 137932 0.44% 143 135414 0.43% 144 136820 0.44% 145 137035 0.44% 146 138474 0.44% 147 143254 0.46% 148 138714 0.44% 149 141108 0.45% 150 138960 0.44% 151 23663138 75.25% 31444949 reads passed initial QC criterion=sequence-density sequence-density=0.99 sequence-density-rank=1 fanout-score=2.31 fanout-score-rank=22 prefix-density=0.99 prefix-fanout=2.3 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=53.87 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=5.9 sequence=AATATCTTTATATATGAACAAAAACTTTTCATGCCCAGCAATTGCTTGGATGCAATGCGGTACTTAGGTACAAAGAGTGAAACATCAGAATAATTAAAGTGGCATGCTTAAAAGGTGTAAAGGCAGCTGCCGTCGTCACTCCTTGCTGTTGGGTCGTAGTTCTCGGCATTCCGGTCAGTGCAACCTTCTGGGACGGGCAAATTACCTTGTTGTGCTCCTTTACCTCCTCCTATGCAGCTAGAGATGGTGTGTGTATGAAGAGTGTTCTAACCGTAGAAGGAACCAGTCTTCATGGCATCTGAGTTAGCATCTCCCAGAGCAGCCTCGCTCATGTACTTGTCAGCAAGCTGCACACGCTTGACATTGTCCTGCTCTTGGACGAGCATGTGGCCGTACTCCAGGAGCTTCTCGATTGTCATCTTTGGCTGCTCAAAGGACACCGGTCCATCCTTCGAGTTCACCAGCTTCTTGCCGATGTTCTCTATTCCGGTTGAGCTGACCCACTTGCGCA criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=3.05 fanout-score-rank=29 prefix-density=0.44 prefix-fanout=2.6 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=41 fanout-score=244.94 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=13.0 sequence=AGAACAAGGAGTGCAAGAAGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTCGAGCACTCGACCGAAGATGTCTTGCTGCGGAGGAAACTGCAACTGCGGGTCATCCTGCAAGTGCGGCAGCGGCTGCAACGGCTGCAACATGTACCCTGAAGCCGAGGTCCAGACCTCCAGCCTCCTCGTCGTCGCCACCGCCGCCCACAAGGCGAGCTCCGGCGGGATGGAGATGGCCGCGGAGAACGGCGGCTGCGGCTGCAGCACCTGCAAGTGC SRR13165375 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:58:52 Started mapping on | Dec 07 16:58:53 Finished on | Dec 07 17:21:45 Mapping speed, Million of reads per hour | 82.51 Number of input reads | 31444949 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 28188265 Uniquely mapped reads % | 89.64% Average mapped length | 285.45 Number of splices: Total | 27410338 Number of splices: Annotated (sjdb) | 25699739 Number of splices: GT/AG | 27028626 Number of splices: GC/AG | 321615 Number of splices: AT/AC | 12598 Number of splices: Non-canonical | 47499 Mismatch rate per base, % | 0.21% Deletion rate per base | 0.01% Deletion average length | 2.60 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1070358 % of reads mapped to multiple loci | 3.40% Number of reads mapped to too many loci | 277206 % of reads mapped to too many loci | 0.88% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.68% % of reads unmapped: other | 3.40% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2186698 2186698 2186698 N_multimapping 1070358 1070358 1070358 N_noFeature 1461265 27432768 1712232 N_ambiguous 617573 4270 113436 UnstrandedReadsAssigned:26109427 PositiveStrandReadsAssigned:751227 NegativeStrandReadsAssigned:26362597 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=139 echo kmer=135 SRR13165375 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165375-trimmed-pair1.fastq SRR13165375-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,444,949 reads, 26,892,902 reads pseudoaligned [quant] estimated average fragment length: 228.822 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,211 rounds 52973 SRR13165375.ke.tsv 35125 SRR13165375.se.tsv 88098 total ==> SRR13165375.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 708.74 0 0 PNS24247 1044 816.178 72.6478 4.64164 PNS24249 1928 1700.18 217.135 6.65993 PNS24246 1044 816.178 72.6478 4.64164 PNS24248 1044 816.178 72.6478 4.64164 PNS24244 1471 1243.18 57.921 2.42961 PNS24243 293 118.806 0 0 KQK14069 1603 1375.18 708.06 26.85 KQK14071 474 263.968 10.6657 2.10704 ==> SRR13165375.se.tsv <== BRADI_1g14170v3 747 BRADI_1g53295v3 211 BRADI_1g59795v3 494 BRADI_1g07683v3 0 BRADI_1g00485v3 7 BRADI_1g20270v3 441 BRADI_1g74790v3 863 BRADI_1g09890v3 0 BRADI_1g77505v3 302 BRADI_1g48960v3 0 SRR13165375 completed mapping pipeline successfully