Starting /dee2/code/volunteer_pipeline.sh SRR13165376 current disk space = 1541704368128 free memory = 1599156192 SRR13165376 SRAfilesize 45cc287bee24cac6b9fa30278c7f529f SRR13165376.sra SRR13165376.sra file validated SRR13165376 is paired end SRR13165376 is conventional basespace SRR13165376 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165376_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.623 37.0 37.0 37.0 37.0 37.0 2 36.06725 37.0 37.0 37.0 37.0 37.0 3 36.482 37.0 37.0 37.0 37.0 37.0 4 36.507 37.0 37.0 37.0 37.0 37.0 5 36.5465 37.0 37.0 37.0 37.0 37.0 6 36.5795 37.0 37.0 37.0 37.0 37.0 7 36.397 37.0 37.0 37.0 37.0 37.0 8 36.589 37.0 37.0 37.0 37.0 37.0 9 36.554 37.0 37.0 37.0 37.0 37.0 10-14 36.5874 37.0 37.0 37.0 37.0 37.0 15-19 36.4465 37.0 37.0 37.0 37.0 37.0 20-24 36.4645 37.0 37.0 37.0 37.0 37.0 25-29 36.454499999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4205 37.0 37.0 37.0 37.0 37.0 35-39 36.433299999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.3407 37.0 37.0 37.0 37.0 37.0 45-49 36.2752 37.0 37.0 37.0 37.0 37.0 50-54 36.310500000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.276599999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.2961 37.0 37.0 37.0 37.0 37.0 65-69 36.2149 37.0 37.0 37.0 37.0 37.0 70-74 36.296299999999995 37.0 37.0 37.0 37.0 37.0 75-79 36.23989999999999 37.0 37.0 37.0 37.0 37.0 80-84 36.2094 37.0 37.0 37.0 37.0 37.0 85-89 36.1588 37.0 37.0 37.0 37.0 37.0 90-94 36.168699999999994 37.0 37.0 37.0 37.0 37.0 95-99 36.1698 37.0 37.0 37.0 37.0 37.0 100-104 36.1272 37.0 37.0 37.0 37.0 37.0 105-109 36.159200000000006 37.0 37.0 37.0 37.0 37.0 110-114 36.0869 37.0 37.0 37.0 37.0 37.0 115-119 36.0235 37.0 37.0 37.0 37.0 37.0 120-124 35.905199999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.921800000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.862700000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.7813 37.0 37.0 37.0 37.0 37.0 140-144 35.6769 37.0 37.0 37.0 37.0 37.0 145-149 35.4418 37.0 37.0 37.0 37.0 37.0 150-151 35.2645 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 1.0 24 2.0 25 4.0 26 5.0 27 8.0 28 17.0 29 18.0 30 26.0 31 42.0 32 53.0 33 83.0 34 189.0 35 320.0 36 2814.0 37 416.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.49999999999999 10.75 4.95 27.800000000000004 2 22.840594308738353 10.652228657768823 32.25887685721481 34.248300176278015 3 20.25 15.775 26.8 37.175000000000004 4 26.924999999999997 18.8 22.575 31.7 5 28.7 28.000000000000004 21.375 21.925 6 24.8 31.525 21.0 22.675 7 18.875 24.7 37.2 19.225 8 19.275000000000002 24.8 31.874999999999996 24.05 9 23.175 19.8 32.925 24.099999999999998 10-14 22.994999999999997 26.935 26.255 23.815 15-19 22.82 25.09 25.945 26.145000000000003 20-24 23.53 26.025 25.45 24.995 25-29 23.48 25.455 25.41 25.655 30-34 23.655 25.155 25.509999999999998 25.679999999999996 35-39 23.335 25.324999999999996 24.985 26.355 40-44 23.77 25.88 24.465 25.885 45-49 23.400000000000002 25.27 24.97 26.36 50-54 23.75 26.16 24.955 25.135 55-59 23.56 25.45 25.295 25.695 60-64 23.599999999999998 25.555 24.845 26.0 65-69 23.44 25.874999999999996 25.235000000000003 25.45 70-74 24.34 25.22 24.240000000000002 26.200000000000003 75-79 24.125 24.69 25.515 25.669999999999998 80-84 23.935000000000002 26.11 24.65 25.305 85-89 24.165 25.455 24.585 25.795 90-94 24.4 25.715 25.025 24.86 95-99 24.645 24.740000000000002 24.535 26.08 100-104 23.575 25.119999999999997 25.180000000000003 26.125 105-109 24.165 26.115 24.895 24.825 110-114 23.765 24.975 25.185000000000002 26.075 115-119 24.75 25.569999999999997 24.48 25.2 120-124 23.875 25.88 24.245 26.0 125-129 24.64 25.09 25.05 25.22 130-134 25.019999999999996 25.75 23.93 25.3 135-139 24.85 25.52 24.169999999999998 25.46 140-144 24.845 24.505 24.915000000000003 25.735000000000003 145-149 24.545 25.45 24.025 25.979999999999997 150-151 24.275 24.587500000000002 25.0 26.137500000000003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.0 24 0.0 25 1.0 26 2.5 27 2.5 28 1.5 29 1.5 30 5.0 31 10.0 32 10.0 33 13.5 34 31.0 35 42.0 36 49.0 37 65.0 38 69.5 39 82.0 40 119.0 41 155.5 42 171.0 43 163.5 44 182.5 45 212.5 46 208.5 47 204.0 48 184.5 49 173.5 50 170.0 51 151.5 52 144.0 53 127.5 54 126.5 55 116.0 56 88.5 57 69.5 58 74.0 59 76.0 60 62.5 61 62.0 62 56.5 63 52.5 64 48.5 65 53.5 66 47.0 67 42.0 68 52.0 69 49.5 70 36.0 71 27.5 72 24.0 73 20.0 74 18.5 75 18.5 76 12.0 77 5.0 78 4.5 79 1.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.7250000000000001 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.825 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89652703186538 50.9 2 17.257429287504475 24.099999999999998 3 6.1582527747941285 12.9 4 2.3988542785535265 6.7 5 0.7518796992481203 2.625 6 0.17901897601145722 0.75 7 0.17901897601145722 0.8750000000000001 8 0.03580379520229145 0.2 9 0.0716075904045829 0.44999999999999996 >10 0.0716075904045829 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTAGTAATCTCGTAT 10 0.25 TruSeq Adapter, Index 11 (97% over 38bp) GGGGTATACTATATTTTAACATCGGAACATCTCACAATACATACACAGGA 10 0.25 No Hit GGCAAACTTCACGGCAATGTGGGAGGTGTGGCAGTCCAGCACTGGGGCGT 9 0.22499999999999998 No Hit CCAGGGTAATCTTGCGAACATATGACTGTCTTCCCAATCCTTTCCGAAAA 9 0.22499999999999998 No Hit GCCAAATATTACAAGGACGTTAGATGTGCCTGACAGGGCACATGAATAGT 8 0.2 No Hit CACGAAACAATCTAATTAACCGACTCACAGTTAGCACGGAGACACACGGC 7 0.17500000000000002 No Hit ATGCATCATGTTGTACATACCCCAATTTTACCCACAAGGACAACACACAC 7 0.17500000000000002 No Hit GGCTCCTCCATCTTCTTCCGCCTCTTCTCCTCCGTCTCTTGGAAGCATAG 7 0.17500000000000002 No Hit GCTAGTATAACGCTAAGAATTTTCCTTCTATTTGATGCGTCAGGCAAATT 7 0.17500000000000002 No Hit GTAGAGTTGATTCATTTCGGTGCGCATATTGGCATAAATATTTGCAACTA 7 0.17500000000000002 No Hit GTCGTTCTCAAGGCTGTAGCTGCAATAGATCCAATCATTTTCCACATGAT 6 0.15 No Hit TCCGCACTTAATTTGGTCCTCTTGGAGTCTAGCTTCTCTTTCCTGGCCTT 6 0.15 No Hit GGTCAAATGGAACGTTGTAGCCATAGTTAGCATTGTTTGAATGAACAATC 6 0.15 No Hit CCTTGCTCTTGGCGGTTTTCAGGCCGTCCTGGATGATGGGCTCGCTCAGC 6 0.15 No Hit TGCACAACCACCAACACCTAGTATTTACACCTATAAAATCCCCGGGGAAG 6 0.15 No Hit GTTCCGTTGCTTCAGAGCAAGAAATCACTATTGTGCTCCCATCACCAGTC 5 0.125 No Hit CCACGACGTTACGCAATCAGCGTCTCAAGCCCATGGACCACAAGTCTTTG 5 0.125 No Hit GTGTGGAAGTAGCAAGGGTTGATGAAAACGGCACAGAACTGGCCGCATTG 5 0.125 No Hit GTCTTGCACCATTTTTCCCTGAATACATGCCTGGTATGTCTAGCGATGTA 5 0.125 No Hit GTCTCACTTCTATGCAACTGGCAAACATGACCATTACACAACGTAGTATT 5 0.125 No Hit GACGAGATCATTTCCTCTTCACAAGGATGCATATTTCGGCACTGGGGTGG 5 0.125 No Hit GACGTCAGGCACGTCGTCACCAGCATCACCGTGATCACCGCCAGCCCGGA 5 0.125 No Hit GCCGGATGTGCTGCTCCCTGGTCTGCATCGCCATGCGGAGCGCGGGCTTG 5 0.125 No Hit CCCTACTAGAGACATATTAGAAAGAAATATAAGTAAACAGGGTAGAAGAG 5 0.125 No Hit GGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTC 5 0.125 No Hit CGACGCTCTTGGAGGAGAGGGTGATGCGGATCCGGTGCTGCACCTCCTGC 5 0.125 No Hit GCTAGAGCCAGATGTTTTACAACACATATCATGACAGGCATTTGCAAACT 5 0.125 No Hit TGCCAATCCATAGATCCCAACACTGACAAGGACGAGCAGAGCCACCGATA 5 0.125 No Hit CAGGCGGTGGTCGGGTCCCTGAACTGCGTCGCGTTCATGCCGGGCTCCGG 5 0.125 No Hit GTCTTGGTTGGTGAACGTTGTAGTTATTGTAGTAGCAGAACTTTGTATCC 5 0.125 No Hit GTCTCTTTTAGCACGGATGCATCACTGGGAGCAGAAATAGGCAATATCGA 5 0.125 No Hit ATAGGTACCTCTGGTTTGTTAAGAAGCGCTAGCCTTCCCATAGGACCTTT 5 0.125 No Hit CACTGGTTGGTCCATTATAAGATATCCACTATATGCATGCCAGACAAAAT 5 0.125 No Hit AGTTATGATTCATGAAAACGGTACGCTATTAGAAAAGAAAAACTTGCCTA 5 0.125 No Hit GTCTAGCATAAGCTGTCAGCAAGCGTCTTAAAGCATTTCTGCCATCCTCA 5 0.125 No Hit ATCTTCTCATACTGTCCTTCACTAACAACTGGCCCAAGCCTGCAACCCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0125 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.1375 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.21250000000000002 0.0 0.0 0.0 0.0 68-69 0.2375 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.3625 0.0 0.0 0.0 0.0 74-75 0.4125 0.0 0.0 0.0 0.0 76-77 0.5125 0.0 0.0 0.0 0.0 78-79 0.65 0.0 0.0 0.0 0.0 80-81 0.8375 0.0 0.0 0.0 0.0 82-83 1.0499999999999998 0.0 0.0 0.0 0.0 84-85 1.1625 0.0 0.0 0.0 0.0 86-87 1.35 0.0 0.0 0.0 0.0 88-89 1.5875 0.0 0.0 0.0 0.0 90-91 1.8625 0.0 0.0 0.0 0.0 92-93 2.275 0.0 0.0 0.0 0.0 94-95 2.55 0.0 0.0 0.0 0.0 96-97 2.8125 0.0 0.0 0.0 0.0 98-99 3.125 0.0 0.0 0.0 0.0 100-101 3.4625 0.0 0.0 0.0 0.0 102-103 3.6875 0.0 0.0 0.0 0.0 104-105 3.9375 0.0 0.0 0.0 0.0 106-107 4.2875 0.0 0.0 0.0 0.0 108-109 4.825 0.0 0.0 0.0 0.0 110-111 5.8875 0.0 0.0 0.0 0.0 112-113 6.325 0.0 0.0 0.0 0.0 114-115 6.987500000000001 0.0 0.0 0.0 0.0 116-117 7.475 0.0 0.0 0.0 0.0 118-119 8.075 0.0 0.0 0.0 0.0 120-121 8.725 0.0 0.0 0.0 0.0 122-123 9.375 0.0 0.0 0.0 0.0 124-125 10.2625 0.0 0.0 0.0 0.0 126-127 10.962499999999999 0.0 0.0 0.0 0.0 128-129 11.45 0.0 0.0 0.0 0.0 130-131 12.1125 0.0 0.0 0.0 0.0 132-133 13.1125 0.0 0.0 0.0 0.0 134-135 13.75 0.0 0.0 0.0 0.0 136-137 14.375 0.0 0.0 0.0 0.0 138-139 14.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACA 10 0.006830828 145.0 6 ATTGGTG 10 0.006830828 145.0 6 GCGGTGT 10 0.006830828 145.0 1 GATTGGT 10 0.006830828 145.0 5 TACAAAG 10 0.006830828 145.0 9 CGGTGTG 10 0.006830828 145.0 2 GCAGATA 10 0.006830828 145.0 1 ATGCACA 10 0.006830828 145.0 9 GTGTGTA 10 0.006830828 145.0 4 TGTGTAC 10 0.006830828 145.0 5 GGTGTGT 10 0.006830828 145.0 3 GGTGCCA 10 0.006830828 145.0 9 >>END_MODULE SRR13165376 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165376_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.043 37.0 37.0 37.0 37.0 37.0 2 36.019 37.0 37.0 37.0 37.0 37.0 3 36.0675 37.0 37.0 37.0 37.0 37.0 4 36.1745 37.0 37.0 37.0 37.0 37.0 5 36.157 37.0 37.0 37.0 37.0 37.0 6 36.187 37.0 37.0 37.0 37.0 37.0 7 36.17 37.0 37.0 37.0 37.0 37.0 8 36.155 37.0 37.0 37.0 37.0 37.0 9 36.1565 37.0 37.0 37.0 37.0 37.0 10-14 36.139599999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.104299999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.057050000000004 37.0 37.0 37.0 37.0 37.0 25-29 35.99145 37.0 37.0 37.0 37.0 37.0 30-34 35.86455 37.0 37.0 37.0 37.0 37.0 35-39 35.89975 37.0 37.0 37.0 37.0 37.0 40-44 35.88845 37.0 37.0 37.0 37.0 37.0 45-49 35.82789999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.74365 37.0 37.0 37.0 37.0 37.0 55-59 35.77355 37.0 37.0 37.0 37.0 37.0 60-64 35.78335 37.0 37.0 37.0 37.0 37.0 65-69 35.7774 37.0 37.0 37.0 37.0 37.0 70-74 35.62075 37.0 37.0 37.0 37.0 37.0 75-79 35.66885 37.0 37.0 37.0 37.0 37.0 80-84 35.648399999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.65965 37.0 37.0 37.0 37.0 37.0 90-94 35.5584 37.0 37.0 37.0 37.0 37.0 95-99 35.66805000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.54585 37.0 37.0 37.0 37.0 37.0 105-109 35.545550000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.372049999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.281949999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.26245 37.0 37.0 37.0 34.6 37.0 125-129 35.0844 37.0 37.0 37.0 27.4 37.0 130-134 34.926249999999996 37.0 37.0 37.0 27.4 37.0 135-139 34.86685 37.0 37.0 37.0 25.0 37.0 140-144 34.599450000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.3664 37.0 37.0 37.0 25.0 37.0 150-151 33.963875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 4.0 15 8.0 16 6.0 17 4.0 18 0.0 19 4.0 20 6.0 21 8.0 22 5.0 23 7.0 24 8.0 25 8.0 26 7.0 27 10.0 28 22.0 29 33.0 30 38.0 31 65.0 32 67.0 33 139.0 34 246.0 35 605.0 36 2488.0 37 208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.1214859437751 21.460843373493976 7.981927710843372 21.43574297188755 2 29.45 22.8 24.25 23.5 3 24.325 23.7 30.7 21.275 4 28.15 29.825000000000003 18.825 23.200000000000003 5 27.224999999999998 33.775 19.375 19.625 6 24.099999999999998 34.425 19.0 22.475 7 23.525 20.375 33.15 22.95 8 24.175 22.775000000000002 23.9 29.15 9 24.8 20.849999999999998 27.750000000000004 26.6 10-14 25.874999999999996 25.46 23.835 24.83 15-19 26.005 25.040000000000003 24.560000000000002 24.395 20-24 25.326266313315664 25.841292064603234 24.07620381019051 24.756237811890593 25-29 26.83670917729432 24.751187796949235 24.051012753188296 24.36109027256814 30-34 26.258938840826122 24.993749062359356 24.43866579986998 24.30864629694454 35-39 26.86134306715336 24.62123106155308 24.286214310715536 24.23121156057803 40-44 26.714007101065164 25.348802320348053 24.628694304145622 23.308496274441165 45-49 26.472647264726472 24.392439243924393 24.792479247924792 24.342434243424343 50-54 25.696284814240713 25.371268563428174 24.8012400620031 24.131206560328017 55-59 26.74168542135534 25.371342835708926 23.940985246311577 23.945986496624155 60-64 26.82134106705335 25.171258562928145 24.666233311665582 23.34116705835292 65-69 27.279999999999998 25.14 24.645 22.935 70-74 26.5666416604151 25.581395348837212 24.38609652413103 23.465866466616657 75-79 26.694004100615093 25.403810571585737 24.158623793569035 23.743561534230135 80-84 26.700000000000003 24.709999999999997 24.51 24.08 85-89 26.721680420105027 25.08627156789197 24.336084021005252 23.85596399099775 90-94 26.877687768776877 24.752475247524753 24.672467246724672 23.697369736973698 95-99 26.651332566628334 25.00125006250313 24.10120506025301 24.24621231061553 100-104 27.536884221055264 25.196299074768692 24.411102775693923 22.85571392848212 105-109 27.60690172543136 24.081020255063766 24.801200300075017 23.510877719429857 110-114 27.011350567528375 25.76128806440322 23.826191309565477 23.401170058502927 115-119 28.35708927231808 24.85621405351338 24.151037759439863 22.635658914728683 120-124 27.991997999499873 24.731182795698924 24.186046511627907 23.090772693173292 125-129 28.575715143028606 24.65493098619724 23.90478095619124 22.86457291458292 130-134 30.043519583812717 25.19133610124556 22.690210594767645 22.074933720174077 135-139 29.845445906067127 25.754013904866703 23.24313509728405 21.157405091782124 140-144 29.976498824941245 24.71623581179059 23.771188559427973 21.536076803840192 145-149 31.339401820546165 23.67210163048915 23.46704011203361 21.52145643693108 150-151 31.232020012507817 24.590368980612883 21.938711694809257 22.238899312070043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.5 23 1.0 24 0.0 25 0.0 26 1.0 27 1.5 28 2.5 29 4.0 30 7.0 31 10.5 32 15.5 33 20.0 34 21.0 35 25.0 36 37.0 37 62.0 38 75.5 39 94.5 40 112.0 41 144.0 42 175.0 43 178.0 44 175.5 45 167.0 46 189.5 47 186.0 48 162.5 49 184.5 50 185.0 51 151.0 52 137.5 53 136.0 54 127.5 55 107.5 56 90.5 57 73.0 58 72.0 59 84.5 60 72.5 61 60.0 62 59.0 63 53.0 64 44.0 65 48.0 66 51.0 67 44.5 68 44.5 69 45.0 70 38.5 71 35.5 72 34.5 73 29.0 74 20.0 75 15.5 76 15.5 77 11.0 78 6.5 79 3.5 80 2.0 81 3.0 82 3.5 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.5 91 1.0 92 2.5 93 2.0 94 0.5 95 2.5 96 3.5 97 3.0 98 3.5 99 3.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.025 90-94 0.01 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.045 135-139 0.034999999999999996 140-144 0.005 145-149 0.03 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 74.01908801696713 52.349999999999994 2 16.82573347472605 23.799999999999997 3 5.655708731000353 12.0 4 2.156238953693885 6.1 5 0.7423117709437964 2.625 6 0.3534817956875221 1.5 7 0.07069635913750442 0.35000000000000003 8 0.03534817956875221 0.2 9 0.07069635913750442 0.44999999999999996 >10 0.07069635913750442 0.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 15 0.375 No Hit GTGAAAGTTCTTATTATGTTATGTCATATTTCCTATGGCAGAATAACACG 10 0.25 No Hit GTCTTCATCATTTTTGACTGCTCAATACTTCATATTGAAATATGTTACCC 9 0.22499999999999998 No Hit GAGGCTCTCCAGGAGGCCCTTCCTGGTGACAATGTTGGCTTCAACGTCAA 9 0.22499999999999998 No Hit CTACCGAGTCGCGACAACGAGCCAGGAGAGGGCGGTGGCCGGTGGGCGCG 8 0.2 No Hit TTGAAGCACATTTCAGCACCACCTCGAAGGATGATGACGCCTCTACCCTC 7 0.17500000000000002 No Hit GTGATGTCAGTCAGGATTGTTGTGTTCTCGCAAGCTGTAGACTATAACAT 7 0.17500000000000002 No Hit GTCCTTTTGGCCACTATCATGCACTCATTGTATGTGTATTCTATGTGGTG 6 0.15 No Hit GTTGCCTAAGAATTTCCTCTCGACCCAATCCTCTCGCCGCCGCCGTAGCA 6 0.15 No Hit GAAGGAGAGAAGTACGTGAAAGCTGTGTTTTCACTGGCAAGCAAAATTTC 6 0.15 No Hit TGGCGCCGAGAATTTTTTTGAAGCTGCAGTTCTCCAAGAGTATGGGCTCA 6 0.15 No Hit GCCAACAAACTCAAACTTACTCCTGCATATCTTGAGCTGAGGTTCATTGA 6 0.15 No Hit CGTCAACAACCTGGAAGGCAACTTCATCTACGCCACCAGGACACTCTTCT 6 0.15 No Hit AAGCAACCCTGATCCCAAATCACAGAGCCACATCACTTCTACTGCCTTGG 6 0.15 No Hit AGAGTTTCTGTGGAAGTGAATTCGAGGAGATACTGTTGTGCGGAGATGAA 6 0.15 No Hit AGTCTGTTGTGAAGCCCATCAACAAGGAGGTGAAGCGTAGGGAGGCTAGG 6 0.15 No Hit GCTAATTTTGTTGGCAACAACCATGCCGATGAGCAAGATAACATCCCTGA 6 0.15 No Hit TATGAAACCGGTAAAAAGATTATGATTGCTGCAGCTCCTACAGTGAAGCC 5 0.125 No Hit GTTCCTGCAGCCACAAACACAGCCTTTCTCTCTCTCAGAAGCTATACTCC 5 0.125 No Hit AATGATCCGAGGCAAGATGCCTCTTTCGGAGTTCAACCTGCCAGATGCCT 5 0.125 No Hit GCCCCCTCGCTCCGTGGATCTTCTTCCTCCTCGCGCGAGCGCCGCCACTC 5 0.125 No Hit GGACCGTTGTACTACATGGGATGGTACCATCTGTTCTACCAATACAACCC 5 0.125 No Hit GGATGGCATGAGGGTGGTGGTGGTGGAGAACCTGAAGAATCCTTATGAGG 5 0.125 No Hit GGTGCACGCTGACTTTGAGTTTTATGGTGGAGCATTTGGGCTTTCTACAT 5 0.125 No Hit CTTATTCTTCTACTAAGTGAGGTTATCCCTAGATGAATGTTTATAGACTT 5 0.125 No Hit CCAGCGCCGCCGCCGCCTCCTCCCTCGCCTCGCGAAACCCTAGCCCGCGG 5 0.125 No Hit GAGATGGCGTTGAGCGGGTGGAGGAGGAGCCCGGCGGCGAGGCGCCCGGC 5 0.125 No Hit GCTGCAATGGCTGCTGAGAGAACTGCATTCCGGGAACATTATCTTTCTCA 5 0.125 No Hit GGCAGCCAGTCCCTCTCTTTGCTGAGCCGGGATCTGCGAGAAGCCCATTT 5 0.125 No Hit GCTCGCGCTCACCACGCTGCGCCCCCCTGGCGAAATTGGGGCCGATATCG 5 0.125 No Hit GCGAGTTGATCAGAGAAAGGGTCAAAGCCACACTGTATAAGTATTACTAC 5 0.125 No Hit CCTTAACCCTCCCACTCGGGCAGCCTGGCACAGTTGAGTTTTCCGAAGAC 5 0.125 No Hit GCAGTTCAGAGCTTCAAATGATACTGTTGAACCAGTGGCTGCAAGTATCA 5 0.125 No Hit ACCCAACCAAGGTTGACCGCAGTGGTGCCTACATTGCCAGGCAGGCCGCC 5 0.125 No Hit GCAACCTGGCCACCGTGTGCGCTGATGGAGCTGTGAGAGTATTCAGGATC 5 0.125 No Hit CTCAAAAAACTTGTTTTTCATTGTCTTTTTTGTTGACACAAGAACAAGAA 5 0.125 No Hit GTGGCCGGTCCTGCTGCTGGCGATCCTGGCATCGATTGTAGGAAGCCAAG 5 0.125 No Hit GTGATACTACAAATGTTCAAAAATAAAACAGAATGGTGTGGTCCTGAAGG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0125 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.1375 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.21250000000000002 0.0 0.0 0.0 0.0 68-69 0.2375 0.0 0.0 0.0 0.0 70-71 0.275 0.0 0.0 0.0 0.0 72-73 0.3625 0.0 0.0 0.0 0.0 74-75 0.4125 0.0 0.0 0.0 0.0 76-77 0.5125 0.0 0.0 0.0 0.0 78-79 0.65 0.0 0.0 0.0 0.0 80-81 0.8375 0.0 0.0 0.0 0.0 82-83 1.0499999999999998 0.0 0.0 0.0 0.0 84-85 1.1625 0.0 0.0 0.0 0.0 86-87 1.35 0.0 0.0 0.0 0.0 88-89 1.6124999999999998 0.0 0.0 0.0 0.0 90-91 1.9 0.0 0.0 0.0 0.0 92-93 2.325 0.0 0.0 0.0 0.0 94-95 2.6 0.0 0.0 0.0 0.0 96-97 2.8625 0.0 0.0 0.0 0.0 98-99 3.175 0.0 0.0 0.0 0.0 100-101 3.4875 0.0 0.0 0.0 0.0 102-103 3.6875 0.0 0.0 0.0 0.0 104-105 3.9625 0.0 0.0 0.0 0.0 106-107 4.3125 0.0 0.0 0.0 0.0 108-109 4.8875 0.0 0.0 0.0 0.0 110-111 6.0 0.0 0.0 0.0 0.0 112-113 6.45 0.0 0.0 0.0 0.0 114-115 7.112500000000001 0.0 0.0 0.0 0.0 116-117 7.6 0.0 0.0 0.0 0.0 118-119 8.2 0.0 0.0 0.0 0.0 120-121 8.85 0.0 0.0 0.0 0.0 122-123 9.475000000000001 0.0 0.0 0.0 0.0 124-125 10.3375 0.0 0.0 0.0 0.0 126-127 11.05 0.0 0.0 0.0 0.0 128-129 11.5375 0.0 0.0 0.0 0.0 130-131 12.162500000000001 0.0 0.0 0.0 0.0 132-133 13.149999999999999 0.0 0.0 0.0 0.0 134-135 13.775 0.0 0.0 0.0 0.0 136-137 14.3875 0.0 0.0 0.0 0.0 138-139 14.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGCC 10 0.006830828 145.0 7 GAAACAG 10 0.006830828 145.0 7 AAACAGT 10 0.006830828 145.0 8 GAGCCAT 10 0.006830828 145.0 9 AACAGTA 10 0.006830828 145.0 9 CTATGCG 10 0.006830828 145.0 2 TATAGCC 10 0.006830828 145.0 145 AAATGGA 10 0.006830828 145.0 2 TGCGGAG 10 0.006830828 145.0 5 GGAGCCA 10 0.006830828 145.0 8 GGAAACA 10 0.006830828 145.0 6 ATGGAAA 20 3.5877043E-4 108.75 4 >>END_MODULE Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451270 spots for SRR13165376.sra Written 1451270 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra Read 1451257 spots for SRR13165376.sra Written 1451257 spots for SRR13165376.sra SRR ids: ['SRR13165376.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_qzqcc20e SRR13165376.sra spots: 29025153 blocks: [[1, 1451257], [1451258, 2902514], [2902515, 4353771], [4353772, 5805028], [5805029, 7256285], [7256286, 8707542], [8707543, 10158799], [10158800, 11610056], [11610057, 13061313], [13061314, 14512570], [14512571, 15963827], [15963828, 17415084], [17415085, 18866341], [18866342, 20317598], [20317599, 21768855], [21768856, 23220112], [23220113, 24671369], [24671370, 26122626], [26122627, 27573883], [27573884, 29025153]] SRR13165376 file size 9842316 SRR13165376 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165376 SRR13165376_1.fastq SRR13165376_2.fastq Input file: SRR13165376_1.fastq Paired file: SRR13165376_2.fastq trimmed: SRR13165376-trimmed-pair1.fastq, SRR13165376-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:46:45 2024 >> started Sat Dec 7 16:47:19 2024 >> done (33.955s) 29025153 read pairs processed; of these: 636 ( 0.00%) short read pairs filtered out after trimming by size control 77675 ( 0.27%) empty read pairs filtered out after trimming by size control 28946842 (99.73%) read pairs available; of these: 5385432 (18.60%) trimmed read pairs available after processing 23561410 (81.40%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 36 0.00% 19 51 0.00% 20 68 0.00% 21 59 0.00% 22 84 0.00% 23 102 0.00% 24 129 0.00% 25 139 0.00% 26 163 0.00% 27 183 0.00% 28 149 0.00% 29 169 0.00% 30 191 0.00% 31 215 0.00% 32 232 0.00% 33 193 0.00% 34 235 0.00% 35 217 0.00% 36 246 0.00% 37 247 0.00% 38 269 0.00% 39 297 0.00% 40 303 0.00% 41 307 0.00% 42 369 0.00% 43 330 0.00% 44 381 0.00% 45 405 0.00% 46 446 0.00% 47 512 0.00% 48 496 0.00% 49 617 0.00% 50 727 0.00% 51 836 0.00% 52 881 0.00% 53 987 0.00% 54 1031 0.00% 55 1116 0.00% 56 1206 0.00% 57 1271 0.00% 58 1462 0.01% 59 1619 0.01% 60 1893 0.01% 61 2443 0.01% 62 2514 0.01% 63 2769 0.01% 64 2940 0.01% 65 3271 0.01% 66 3580 0.01% 67 4005 0.01% 68 4262 0.01% 69 5025 0.02% 70 5535 0.02% 71 6385 0.02% 72 7126 0.02% 73 8162 0.03% 74 8774 0.03% 75 9780 0.03% 76 10905 0.04% 77 11534 0.04% 78 12796 0.04% 79 13964 0.05% 80 15477 0.05% 81 16943 0.06% 82 18671 0.06% 83 20534 0.07% 84 22239 0.08% 85 25010 0.09% 86 27053 0.09% 87 27919 0.10% 88 29717 0.10% 89 30635 0.11% 90 32376 0.11% 91 35259 0.12% 92 37287 0.13% 93 39349 0.14% 94 42162 0.15% 95 44644 0.15% 96 46980 0.16% 97 49108 0.17% 98 50642 0.17% 99 53272 0.18% 100 53794 0.19% 101 55454 0.19% 102 56604 0.20% 103 59330 0.20% 104 61605 0.21% 105 62644 0.22% 106 66146 0.23% 107 67894 0.23% 108 68920 0.24% 109 70470 0.24% 110 71505 0.25% 111 72562 0.25% 112 74315 0.26% 113 75114 0.26% 114 77934 0.27% 115 79421 0.27% 116 81423 0.28% 117 82376 0.28% 118 84583 0.29% 119 84760 0.29% 120 86288 0.30% 121 87553 0.30% 122 88259 0.30% 123 90491 0.31% 124 91103 0.31% 125 91571 0.32% 126 93513 0.32% 127 94925 0.33% 128 96327 0.33% 129 97506 0.34% 130 98402 0.34% 131 98780 0.34% 132 99978 0.35% 133 101476 0.35% 134 101156 0.35% 135 102389 0.35% 136 103394 0.36% 137 104463 0.36% 138 105386 0.36% 139 107757 0.37% 140 108280 0.37% 141 109470 0.38% 142 110610 0.38% 143 109123 0.38% 144 110682 0.38% 145 111497 0.39% 146 112152 0.39% 147 113788 0.39% 148 112010 0.39% 149 113490 0.39% 150 114843 0.40% 151 23561410 81.40% 28946842 reads passed initial QC criterion=sequence-density sequence-density=0.15 sequence-density-rank=1 fanout-score=21.13 fanout-score-rank=6 prefix-density=0.34 prefix-fanout=9.3 sequence=TCTCCAGCTCCTT criterion=fanout-score sequence-density=0.05 sequence-density-rank=28 fanout-score=171.67 fanout-score-rank=1 prefix-density=0.59 prefix-fanout=16.0 sequence=GGCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAG criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=2.25 fanout-score-rank=37 prefix-density=0.23 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.05 sequence-density-rank=32 fanout-score=274.32 fanout-score-rank=1 prefix-density=0.98 prefix-fanout=13.5 sequence=AAGAAGAAGGTGGAGTCCAAGAACGCCCTGGAGAACTACTCGTACAACATGCGCAACACCATCAAGGACGAGAAGATCGCCTCCAAGCTGCCGGCGGACGACAAGAAGAAGATCGAGGACGCCATTGA SRR13165376 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:48:36 Started mapping on | Dec 07 16:48:36 Finished on | Dec 07 16:51:34 Mapping speed, Million of reads per hour | 585.44 Number of input reads | 28946842 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 26833859 Uniquely mapped reads % | 92.70% Average mapped length | 289.91 Number of splices: Total | 25116869 Number of splices: Annotated (sjdb) | 23305234 Number of splices: GT/AG | 24731328 Number of splices: GC/AG | 323339 Number of splices: AT/AC | 15527 Number of splices: Non-canonical | 46675 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.01% Deletion average length | 2.36 Insertion rate per base | 0.01% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 343466 % of reads mapped to multiple loci | 1.19% Number of reads mapped to too many loci | 151423 % of reads mapped to too many loci | 0.52% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.03% % of reads unmapped: other | 2.56% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1769848 1769848 1769848 N_multimapping 343466 343466 343466 N_noFeature 1243790 26057906 1518569 N_ambiguous 593980 3406 93397 UnstrandedReadsAssigned:24996089 PositiveStrandReadsAssigned:772547 NegativeStrandReadsAssigned:25221893 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13165376 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165376-trimmed-pair1.fastq SRR13165376-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 28,946,842 reads, 25,656,626 reads pseudoaligned [quant] estimated average fragment length: 242.193 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,190 rounds 52973 SRR13165376.ke.tsv 35125 SRR13165376.se.tsv 88098 total ==> SRR13165376.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 695.327 0 0 PNS24247 1044 802.807 123.062 9.09572 PNS24249 1928 1686.81 224.856 7.90973 PNS24246 1044 802.807 123.062 9.09572 PNS24248 1044 802.807 123.062 9.09572 PNS24244 1471 1229.81 304.958 14.7139 PNS24243 293 109.803 0 0 KQK14069 1603 1361.81 33311 1451.43 KQK14071 474 253.59 386.375 90.4067 ==> SRR13165376.se.tsv <== BRADI_1g14170v3 35106 BRADI_1g53295v3 486 BRADI_1g59795v3 1145 BRADI_1g07683v3 0 BRADI_1g00485v3 17 BRADI_1g20270v3 952 BRADI_1g74790v3 709 BRADI_1g09890v3 0 BRADI_1g77505v3 430 BRADI_1g48960v3 0 SRR13165376 completed mapping pipeline successfully