Starting /dee2/code/volunteer_pipeline.sh SRR13165378 current disk space = 1541562986496 free memory = 1475880268 SRR13165378 SRAfilesize ba3981767763ad767f00dd1e91606203 SRR13165378.sra SRR13165378.sra file validated SRR13165378 is paired end SRR13165378 is conventional basespace SRR13165378 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165378_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.557 37.0 37.0 37.0 37.0 37.0 2 36.1815 37.0 37.0 37.0 37.0 37.0 3 36.478 37.0 37.0 37.0 37.0 37.0 4 36.4595 37.0 37.0 37.0 37.0 37.0 5 36.6045 37.0 37.0 37.0 37.0 37.0 6 36.5005 37.0 37.0 37.0 37.0 37.0 7 36.2905 37.0 37.0 37.0 37.0 37.0 8 36.552 37.0 37.0 37.0 37.0 37.0 9 36.39 37.0 37.0 37.0 37.0 37.0 10-14 36.5103 37.0 37.0 37.0 37.0 37.0 15-19 36.4849 37.0 37.0 37.0 37.0 37.0 20-24 36.46320000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.3909 37.0 37.0 37.0 37.0 37.0 30-34 36.4121 37.0 37.0 37.0 37.0 37.0 35-39 36.3974 37.0 37.0 37.0 37.0 37.0 40-44 36.37169999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.337900000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.2674 37.0 37.0 37.0 37.0 37.0 55-59 36.2943 37.0 37.0 37.0 37.0 37.0 60-64 36.2813 37.0 37.0 37.0 37.0 37.0 65-69 36.254599999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.217200000000005 37.0 37.0 37.0 37.0 37.0 75-79 36.2355 37.0 37.0 37.0 37.0 37.0 80-84 36.1108 37.0 37.0 37.0 37.0 37.0 85-89 36.095099999999995 37.0 37.0 37.0 37.0 37.0 90-94 36.1041 37.0 37.0 37.0 37.0 37.0 95-99 36.106700000000004 37.0 37.0 37.0 37.0 37.0 100-104 36.0765 37.0 37.0 37.0 37.0 37.0 105-109 36.137 37.0 37.0 37.0 37.0 37.0 110-114 36.0972 37.0 37.0 37.0 37.0 37.0 115-119 36.051300000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.893299999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.8839 37.0 37.0 37.0 37.0 37.0 130-134 35.8743 37.0 37.0 37.0 37.0 37.0 135-139 35.78680000000001 37.0 37.0 37.0 37.0 37.0 140-144 35.7676 37.0 37.0 37.0 37.0 37.0 145-149 35.486900000000006 37.0 37.0 37.0 37.0 37.0 150-151 35.29375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 1.0 24 2.0 25 2.0 26 9.0 27 9.0 28 9.0 29 20.0 30 30.0 31 61.0 32 60.0 33 78.0 34 151.0 35 337.0 36 2843.0 37 386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.125 10.5 4.05 23.325000000000003 2 22.40080361627323 9.919638372677046 32.59668508287293 35.0828729281768 3 19.85 17.25 27.625 35.275 4 24.975 22.275 23.875 28.875 5 27.725 26.174999999999997 23.1 23.0 6 22.2 31.275 21.85 24.675 7 18.224999999999998 25.0 38.324999999999996 18.45 8 18.825 24.05 31.0 26.125 9 20.0 21.475 33.900000000000006 24.625 10-14 23.205000000000002 26.63 25.345000000000002 24.82 15-19 22.509999999999998 25.495 26.39 25.605 20-24 23.615 25.835 25.629999999999995 24.92 25-29 24.09 25.095 25.53 25.285000000000004 30-34 22.665 26.005 25.765 25.564999999999998 35-39 22.17 25.779999999999998 25.4 26.650000000000002 40-44 23.11 25.56 25.405 25.924999999999997 45-49 23.905 24.89 26.44 24.765 50-54 22.705000000000002 25.369999999999997 25.919999999999998 26.005 55-59 23.535 25.245 26.224999999999998 24.995 60-64 23.25 25.845000000000002 25.56 25.345000000000002 65-69 23.945 25.855 25.224999999999998 24.975 70-74 23.06 25.765 25.655 25.52 75-79 23.845 24.834999999999997 25.580000000000002 25.740000000000002 80-84 23.630000000000003 25.095 25.91 25.365 85-89 24.3 24.68 25.97 25.05 90-94 24.395 25.56 24.765 25.28 95-99 23.935000000000002 25.369999999999997 24.88 25.814999999999998 100-104 23.580000000000002 26.3 25.15 24.97 105-109 24.65 25.505 24.044999999999998 25.8 110-114 23.735 25.03 24.42 26.815 115-119 23.794999999999998 26.724999999999998 23.435 26.045 120-124 23.98 26.115 24.075 25.83 125-129 24.38 25.040000000000003 24.725 25.855 130-134 23.71 25.555 24.755 25.979999999999997 135-139 23.22 26.474999999999998 24.035 26.27 140-144 24.245 24.945 24.845 25.965 145-149 24.36 24.33 24.435000000000002 26.875 150-151 23.1 23.9 25.362499999999997 27.6375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5 24 0.5 25 0.5 26 2.0 27 2.5 28 3.0 29 5.5 30 9.0 31 13.5 32 12.5 33 18.5 34 27.0 35 44.0 36 62.5 37 72.0 38 88.5 39 97.0 40 114.5 41 154.0 42 163.5 43 173.5 44 194.0 45 196.5 46 204.0 47 207.0 48 187.5 49 175.5 50 184.0 51 160.0 52 137.5 53 123.0 54 116.5 55 110.5 56 88.5 57 67.0 58 60.0 59 69.0 60 66.5 61 62.5 62 61.0 63 54.5 64 44.0 65 39.0 66 45.5 67 50.5 68 39.5 69 30.0 70 29.0 71 37.5 72 39.5 73 23.0 74 12.5 75 7.5 76 4.5 77 3.0 78 2.5 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.325 #Duplication Level Percentage of deduplicated Percentage of total 1 70.03341997772002 47.15 2 18.863720757519495 25.4 3 6.75826216115856 13.65 4 2.8592647604901598 7.7 5 0.7055328629780914 2.375 6 0.40846639435573706 1.6500000000000001 7 0.18566654288897141 0.8750000000000001 8 0.07426661715558856 0.4 9 0.03713330857779428 0.22499999999999998 >10 0.07426661715558856 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACTTTGAATCAAGATGATCAACATCCTTCAAGACAGAAAGAAGGTGTT 13 0.325 No Hit GAAACAGCATCAGATACCAACTTTTCTCCTTGAGTTCTTGCCTTCTTGGT 10 0.25 No Hit AGTCCGTCTGCAGCCACTCGCCGCACTTGTAGCCGCCGCTGGTGGAGCGC 9 0.22499999999999998 No Hit GTTCTTGGTATGCTCAACAATAGCACATAAATCACTGGAGAAGAATAATT 8 0.2 No Hit CACTGAGTAACAATCAGCCCTTCACTTCTCTGAGAAATCCCATGGTGCGA 8 0.2 No Hit GGCTGCAGCATCGCCACCTTGCGCGCTCTCCCGGGGTGCAGATCCAGAAG 7 0.17500000000000002 No Hit ACACCATCCAGCCCACATGTCGTCGTAGCGACCAATAGGCTGGCCATCTC 7 0.17500000000000002 No Hit TGGTGCTGCAGGATTCATAAATATCTGACTCATTAGCTCAACAACATCTT 7 0.17500000000000002 No Hit GGTTTCTTTTAACTTGGATACAATGATAACCGTACTTGTAGCCACTCTAA 7 0.17500000000000002 No Hit GGTCGATCCTCTGCTCCAGGTCGGTGCAGCAGCAGCTCCGCCTCATCAGA 7 0.17500000000000002 No Hit GCCCGTTGTTGTAGAACTCGTTGACGAGCTCCGAGAACTGCGCGAACATG 6 0.15 No Hit ATCCGCGCGGGGGGTCGGTAGGGGTCGCCGACCTCGATCTTGTAGGAGAC 6 0.15 No Hit CTGGCCTAAAAGCATCCTCTTCCAAAGCCTTGCAAATCTTTCTGATGCGG 6 0.15 No Hit GCGCCTCAGTACTCTGCTCCCAGGAGGGCAAGGAGCATATCCTCGTAGTC 6 0.15 No Hit CAGAGCGTACGTGCAATATTTCTGACCCTGAACCAAAATGTACCCATCTG 6 0.15 No Hit GAATGCTATAACATATGGGTTTACTCCTGTGACACAGCTTATCTGATCTG 6 0.15 No Hit GGCAACAACAGTTTTTTCTCTGGCAGAAAAGGACAGCAGTAACAAAGGCG 6 0.15 No Hit GTCGTCCTCACCACCACCCTCAGCATGCTTGCCTTTCTCCAACCTCTTGA 6 0.15 No Hit GGGTGCTGTAGAGAGTCGTACTTTTTTGCAGTGCTTCCTTCTCGTCCAAC 6 0.15 No Hit AGCAGAAGTCTTGAAGAAGACAGAGGCTTTCTTCTTGTCCAGCCCAGTCA 6 0.15 No Hit GTCTACACAATTCATCAAGTTCGATGGTTTTCATCACTAGGCAGTAGGTA 6 0.15 No Hit CCCAGATTGGCCGCAGCCAGTACTCAGGTTATCTGAAGTACATGATATCT 5 0.125 No Hit GGACTCTGGGAAGCAGGTTGAGATATGCTCAAGTTGAGGTCGACATCTGC 5 0.125 No Hit GCCATGAATCTGAATCACCAGAAGTGTACACTGTACAAGGACTATCAAGA 5 0.125 No Hit GGCTTCTCCTGCACCACGCGGCAGGCCAAGCAGTTGGGGTGGCACTTGGC 5 0.125 No Hit GCCTCTTCGGGGAAATTGACCTTTGCCTTCTTGCCACGAATCCTGCGTGC 5 0.125 No Hit GCCCACTTGTTATTCTTATCAGCAATGTCAGTCATGCTTTTCCATGTATC 5 0.125 No Hit AAGGCATCTGTGCGCAACAAATATTAATAGATTGGGTAATACTATTATTC 5 0.125 No Hit GCCACAGTTGCAGCTTGATCCACAGCTGCAAGACATCTTCCAAATCTCTC 5 0.125 No Hit ATGAACGGGATGCCAATCTCATCAGCAAATGCCTTTGCTGTCTCATACGA 5 0.125 No Hit CTCGACAAGAATCTTCAACACTGATCTGCTCTCCAGCCAGACTTCCTGTC 5 0.125 No Hit GCTGTTACACCACATACATCGGAAGAGAATTGAATATTGGAGATATTGAG 5 0.125 No Hit CACTATACAATTCTCCACAGCTACACTTTTGAGCGGCAGAACTTCATCAC 5 0.125 No Hit GCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCC 5 0.125 No Hit GGGGATAAAAGACAAGATGGATCAGATTTCTGGCTTGGAATCCTGATGGG 5 0.125 No Hit GTCACTTCTACAAACCTTGTATTCCATCTTTCCAGTTGCCGGATCAAGTA 5 0.125 No Hit GTTCTCTGGATACCCCTTTCTTGTTCCTGCTCAGATTTAACCAGACTGGT 5 0.125 No Hit GTTGTTGAAGCCAATAGTAACAGCCAGGCAGAGTATCATCAGGATCCAAT 5 0.125 No Hit GGACGACTTTGAGCGGGGCACCGTCGCCGATGACCAGCGATTTCAGCCAT 5 0.125 No Hit GCCAACTTTCCAAAGTAGCGGTCCAAGTTGTGCCACTGAGGATCCTTGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.2 0.0 0.0 0.0 0.0 58-59 0.21250000000000002 0.0 0.0 0.0 0.0 60-61 0.225 0.0 0.0 0.0 0.0 62-63 0.225 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.275 0.0 0.0 0.0 0.0 68-69 0.3625 0.0 0.0 0.0 0.0 70-71 0.4 0.0 0.0 0.0 0.0 72-73 0.425 0.0 0.0 0.0 0.0 74-75 0.5 0.0 0.0 0.0 0.0 76-77 0.5625 0.0 0.0 0.0 0.0 78-79 0.65 0.0 0.0 0.0 0.0 80-81 0.775 0.0 0.0 0.0 0.0 82-83 0.8875 0.0 0.0 0.0 0.0 84-85 1.0 0.0 0.0 0.0 0.0 86-87 1.2000000000000002 0.0 0.0 0.0 0.0 88-89 1.5125000000000002 0.0 0.0 0.0 0.0 90-91 1.75 0.0 0.0 0.0 0.0 92-93 2.2125 0.0 0.0 0.0 0.0 94-95 2.7 0.0 0.0 0.0 0.0 96-97 3.0875 0.0 0.0 0.0 0.0 98-99 3.65 0.0 0.0 0.0 0.0 100-101 4.475 0.0 0.0 0.0 0.0 102-103 4.887499999999999 0.0 0.0 0.0 0.0 104-105 5.324999999999999 0.0 0.0 0.0 0.0 106-107 6.0375 0.0 0.0 0.0 0.0 108-109 6.675000000000001 0.0 0.0 0.0 0.0 110-111 7.275 0.0 0.0 0.0 0.0 112-113 8.1875 0.0 0.0 0.0 0.0 114-115 9.1125 0.0 0.0 0.0 0.0 116-117 10.15 0.0 0.0 0.0 0.0 118-119 10.962499999999999 0.0 0.0 0.0 0.0 120-121 11.6125 0.0 0.0 0.0 0.0 122-123 12.275 0.0 0.0 0.0 0.0 124-125 13.212499999999999 0.0 0.0 0.0 0.0 126-127 14.100000000000001 0.0 0.0 0.0 0.0 128-129 14.8375 0.0 0.0 0.0 0.0 130-131 15.4625 0.0 0.0 0.0 0.0 132-133 16.2875 0.0 0.0 0.0 0.0 134-135 16.9375 0.0 0.0 0.0 0.0 136-137 17.625 0.0 0.0 0.0 0.0 138-139 18.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAAGGC 10 0.006830828 145.0 5 ACCATTC 10 0.006830828 145.0 9 GCTATCA 10 0.006830828 145.0 3 GGCCCTC 10 0.006830828 145.0 9 AGAGCAG 10 0.006830828 145.0 5 AGGCCCT 10 0.006830828 145.0 8 CACCATT 10 0.006830828 145.0 8 AAGGCCC 10 0.006830828 145.0 7 TGCTATC 10 0.006830828 145.0 2 TATCACC 10 0.006830828 145.0 5 TCACCAT 10 0.006830828 145.0 7 ATCAAGG 10 0.006830828 145.0 4 CTATCAC 10 0.006830828 145.0 4 GTGCTAT 15 1.1411342E-4 145.0 1 >>END_MODULE SRR13165378 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165378_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.04625 37.0 37.0 37.0 37.0 37.0 2 35.979 37.0 37.0 37.0 37.0 37.0 3 35.9205 37.0 37.0 37.0 37.0 37.0 4 35.988 37.0 37.0 37.0 37.0 37.0 5 36.1495 37.0 37.0 37.0 37.0 37.0 6 36.1205 37.0 37.0 37.0 37.0 37.0 7 36.0575 37.0 37.0 37.0 37.0 37.0 8 36.067 37.0 37.0 37.0 37.0 37.0 9 36.135 37.0 37.0 37.0 37.0 37.0 10-14 36.043099999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.095099999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.019850000000005 37.0 37.0 37.0 37.0 37.0 25-29 35.95995 37.0 37.0 37.0 37.0 37.0 30-34 35.948449999999994 37.0 37.0 37.0 37.0 37.0 35-39 35.892649999999996 37.0 37.0 37.0 37.0 37.0 40-44 35.903949999999995 37.0 37.0 37.0 37.0 37.0 45-49 35.89525 37.0 37.0 37.0 37.0 37.0 50-54 35.83055 37.0 37.0 37.0 37.0 37.0 55-59 35.86045 37.0 37.0 37.0 37.0 37.0 60-64 35.77885 37.0 37.0 37.0 37.0 37.0 65-69 35.68845 37.0 37.0 37.0 37.0 37.0 70-74 35.68275 37.0 37.0 37.0 37.0 37.0 75-79 35.72705 37.0 37.0 37.0 37.0 37.0 80-84 35.67095 37.0 37.0 37.0 37.0 37.0 85-89 35.611450000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.53745 37.0 37.0 37.0 37.0 37.0 95-99 35.61465 37.0 37.0 37.0 37.0 37.0 100-104 35.595349999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.49165000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.378949999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.37075 37.0 37.0 37.0 37.0 37.0 120-124 35.294050000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.28585 37.0 37.0 37.0 37.0 37.0 130-134 35.04185 37.0 37.0 37.0 29.8 37.0 135-139 34.908550000000005 37.0 37.0 37.0 27.4 37.0 140-144 34.661350000000006 37.0 37.0 37.0 25.0 37.0 145-149 34.44905 37.0 37.0 37.0 25.0 37.0 150-151 34.0715 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 5.0 14 7.0 15 7.0 16 4.0 17 1.0 18 3.0 19 6.0 20 3.0 21 8.0 22 15.0 23 8.0 24 3.0 25 9.0 26 10.0 27 16.0 28 19.0 29 17.0 30 38.0 31 45.0 32 84.0 33 116.0 34 252.0 35 550.0 36 2531.0 37 242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.048859934853425 20.871961914307192 5.437233775995991 18.641944374843398 2 28.075 21.05 29.225 21.65 3 25.0 21.525 31.324999999999996 22.15 4 27.35 31.900000000000002 18.875 21.875 5 28.849999999999998 33.45 19.7 18.0 6 23.525 35.275 18.125 23.075000000000003 7 24.95 19.1 33.550000000000004 22.400000000000002 8 23.825 21.875 27.325 26.974999999999998 9 24.675 22.45 25.674999999999997 27.200000000000003 10-14 27.22 25.855 22.615 24.310000000000002 15-19 26.424999999999997 24.66 24.47 24.445 20-24 25.621405351337835 25.156289072268066 24.60615153788447 24.616154038509627 25-29 25.906476619154787 25.55138784696174 23.970992748187047 24.571142785696424 30-34 26.301575393848463 25.116279069767444 24.351087771942986 24.23105776444111 35-39 25.621405351337835 25.061265316329084 24.61115278819705 24.706176544136035 40-44 26.81670417604401 25.561390347586897 23.265816454113526 24.356089022255563 45-49 25.61140285071268 25.566391597899475 24.776194048512128 24.046011502875718 50-54 25.331332833208304 25.52638159539885 24.751187796949235 24.391097774443608 55-59 25.726431607901972 25.301325331332837 24.36109027256814 24.61115278819705 60-64 25.626406601650416 25.731432858214554 25.07126781695424 23.570892723180794 65-69 26.293944091613742 26.048907336100413 24.208631294694204 23.448517277591638 70-74 26.60665166291573 24.776194048512128 24.726181545386346 23.890972743185795 75-79 25.646411602900727 25.42135533883471 25.10627656914228 23.82595648912228 80-84 26.331582895723933 25.26631657914479 24.786196549137284 23.615903975993998 85-89 26.556639159789945 24.66616654163541 24.436109027256812 24.34108527131783 90-94 26.466616654163538 24.801200300075017 24.976244061015255 23.755938984746187 95-99 25.916479119779943 25.68642160540135 24.8062015503876 23.59089772443111 100-104 26.716679169792446 25.511377844461116 25.1262815703926 22.64566141535384 105-109 27.24181045261315 24.63115778944736 24.36109027256814 23.765941485371343 110-114 26.851712928232057 26.66166541635409 23.88097024256064 22.605651412853213 115-119 27.246811702925733 26.576644161040257 24.06101525381345 22.115528882220556 120-124 28.132033008252062 25.63640910227557 24.111027756939237 22.120530132533133 125-129 27.421855463865967 25.496374093523382 24.376094023505875 22.705676419104776 130-134 29.203141413636136 25.726576959631835 24.060827372317544 21.009454254414486 135-139 28.6600310108538 24.943730305606962 24.70864802680938 21.687590656729856 140-144 30.017504376094024 24.77119279819955 24.326081520380093 20.885221305326333 145-149 30.445655979592857 24.933726804381536 23.54324013404692 21.077377081978693 150-151 30.860645484113086 25.193895421566175 24.06805103827871 19.877408056042032 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.5 10 0.5 11 0.5 12 0.5 13 0.5 14 0.5 15 0.5 16 1.0 17 2.0 18 1.5 19 0.0 20 1.0 21 2.5 22 2.5 23 2.0 24 2.0 25 2.0 26 1.0 27 2.0 28 3.0 29 4.5 30 10.0 31 13.0 32 16.0 33 19.0 34 25.0 35 36.0 36 39.0 37 51.0 38 64.0 39 66.5 40 96.0 41 137.0 42 167.0 43 198.0 44 193.0 45 198.0 46 213.0 47 189.0 48 159.0 49 163.5 50 165.5 51 150.0 52 148.0 53 112.0 54 90.5 55 103.5 56 95.0 57 91.0 58 93.0 59 81.0 60 71.5 61 65.5 62 58.0 63 57.5 64 58.0 65 54.0 66 48.0 67 48.5 68 45.0 69 42.5 70 46.5 71 44.0 72 33.0 73 27.0 74 24.5 75 14.0 76 10.5 77 7.5 78 5.5 79 2.5 80 0.5 81 0.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 1.0 90 1.5 91 1.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 1.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.015 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.045 135-139 0.034999999999999996 140-144 0.025 145-149 0.034999999999999996 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.925 #Duplication Level Percentage of deduplicated Percentage of total 1 70.887007729113 48.15 2 18.58667648141332 25.25 3 6.256900993743099 12.75 4 2.6499815973500183 7.199999999999999 5 0.7729112992270887 2.625 6 0.44166359955833645 1.7999999999999998 7 0.22083179977916823 1.05 8 0.11041589988958411 0.6 9 0.0 0.0 >10 0.0736105999263894 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCGTCACCGCCGGCCCATCTTCGCCGGCTCGCCTCGCCTCGATACATGA 13 0.325 No Hit GGAAAAGGGAAAAAAGGAGGATCAGAAGGGGATGGAAATGGTGAGGAGGA 10 0.25 No Hit GTGACATTTAGCCAAGTCGTTATGGAAGTTATGAGGCAACTTGAAGGAGC 8 0.2 No Hit ATCAAGAACCCCAATCCAAAGCCCAACCCGAAAAAATTCCAATAAATTCT 8 0.2 No Hit GCTCATCATCTTGTTTAATACCAAAGCTCTTCATATTCTCCTCCTTGATT 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 7 0.17500000000000002 No Hit GTTGGAATGGAACCAGAGGTGCCGCTGCCGGCGGTGGAGACGGGAACGGC 7 0.17500000000000002 No Hit GTTTTAGTCTCTGTTTTTCCTTATGAGGAGGCAGCTAAATTATGTGGAGG 7 0.17500000000000002 No Hit CAGCAGGTATGTTGATGCTGTCCTGACTATTCCAAAGGGAACCCTTTTCC 7 0.17500000000000002 No Hit AAGAAGGCAAATTAAGCGCGCGACACGAAGCCACCGCAAGAACTTTTTCA 7 0.17500000000000002 No Hit CTGCTACCATACGCACTCTCGCATCTGGACAAGAAGATGGCTGGAACTTT 7 0.17500000000000002 No Hit CTCTGGCACACTCTGAAGCCAAGATACTCCATGAGAAGATCAATGATGGG 6 0.15 No Hit GAAGTTCTTGGTGGATACATCTTTGTCTGCAACAATGATACCATGCAGGA 6 0.15 No Hit CCTTGTGTTCGTCGCACCGCCTTTTCTCTTTCTCAGATCTGCTCTTTCTC 6 0.15 No Hit GTTCTACAGGGCGGAGGAGTACCACCAACAGTACCTGGAGAAGGGCGGTC 6 0.15 No Hit GCTTATTGTACCGAGTGTGCAATTGATGGTAATAAATTTGTAGATGTACT 6 0.15 No Hit GGTATCATCATGTTCCAGAAAAGGATATAGAAGAATCATCAAAAACGACT 6 0.15 No Hit GCGAGAACCAGGCGGTAACAAGGTGGGAAGGTGATATCATGGATTTCAAG 6 0.15 No Hit CCTGCTCGAGATCGCCGCGCGCTCCGATGTCGTGACCGTCGTCCGTTGCG 6 0.15 No Hit TCAAACTGGGATATCTACCATTGATGTCATGAACTCCATTGCTCGAGGGC 6 0.15 No Hit AGGAGAGGGAATCTTTTGCAGAGAAACTCAGTGAGGTGCAAGATTGGTTG 6 0.15 No Hit CTGCAAGCACCACTGATCAGATCTCAGATGAGATGGCCCAACGAGATGCA 6 0.15 No Hit CCGTGGGCTCTGCGCTCGCGGCCACCGTGCTGTTTGACTGCAATGTCCTC 6 0.15 No Hit GCATGATCAGTCTAGCTGTATGTGCACTTAAATCCTTGTACTGCTGTTAG 5 0.125 No Hit CTGATGACGGACCTGTTTTAGGAAGAAAAGAAAAGGAGAACAATCAAAAA 5 0.125 No Hit GGAAGAGAAAGAACCAATTCAGGGGCATTCGACGCCGCCCTTGGGGTAAA 5 0.125 No Hit AGCGCATGGGCCAGTTCCTCGGCAAGAAGTACATATATCTTGGGCTATTC 5 0.125 No Hit TGAAAGTATACATAGGTGGATGCTTGTTTATGGGTTTGTCATCCATTATA 5 0.125 No Hit AATCCTTCAGCTGATCTGAAGTGCAAAATGTGTGTTCAGGAGTTCTCTGA 5 0.125 No Hit GTCGTGTATGTATCTTCTTGAGGATATCATAATTCTCCTTGAGGTCATAT 5 0.125 No Hit GGATGATATCATTGAAGAACTTGTCACTAAGGGGCAACAACTTGATGCAG 5 0.125 No Hit TGTACCACAAGATGCAGTCCAAAAGCATCCAGTGCCCGGCCGACAGCGAC 5 0.125 No Hit ATTGGAGTACGCCTCGCGGCTTCGCGATCTCGCCATGAATCCCGAGTATG 5 0.125 No Hit GCCAAAGGAAAGGTATCCTCCAATGCATGATGCTCTAAATACAACTGGGC 5 0.125 No Hit ATTGTGTCCGAGCTCTCCAACAAGGACTTTGACTCCTATGTTCTTTCTGA 5 0.125 No Hit ATACTATAGTTAAGGGTTCCAATCTGATGCGTTCTCTGTCGAAGGCTAGC 5 0.125 No Hit GTTCTATTTCGTGCTGGTGATATTCTAATTCTGGATCTCAGGGACCCAAA 5 0.125 No Hit GGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCT 5 0.125 No Hit GGAAAGTAGTGATAAAGCTCCTGAAAAACCTCCAATGTTGAAAGGCCCCA 5 0.125 No Hit GTAGACCGGACAAGGCGCGGCAGCTTTGCGCGGAAATCTAAGAAGCAACG 5 0.125 No Hit CCCAAGTCTGATCCGGCCATGGCCTCGAACGGGAGCAGCACGACGGTGGG 5 0.125 No Hit TTGTACTAAAACCACGACAAGAATTTAGGACCGGAGGGAGTAGCTGTAGG 5 0.125 No Hit GAAAGATGAGATGTGTGCGTTTGATTTGCTTGCGACCGTTGCAGGAACAC 5 0.125 No Hit CTTCTCTGTGTAACAGGCTCAGAAGCTATGTACGCAGATCTTGGACATTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.2 0.0 0.0 0.0 0.0 58-59 0.21250000000000002 0.0 0.0 0.0 0.0 60-61 0.225 0.0 0.0 0.0 0.0 62-63 0.225 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.275 0.0 0.0 0.0 0.0 68-69 0.3625 0.0 0.0 0.0 0.0 70-71 0.4 0.0 0.0 0.0 0.0 72-73 0.425 0.0 0.0 0.0 0.0 74-75 0.5 0.0 0.0 0.0 0.0 76-77 0.575 0.0 0.0 0.0 0.0 78-79 0.675 0.0 0.0 0.0 0.0 80-81 0.8 0.0 0.0 0.0 0.0 82-83 0.9125 0.0 0.0 0.0 0.0 84-85 1.0499999999999998 0.0 0.0 0.0 0.0 86-87 1.25 0.0 0.0 0.0 0.0 88-89 1.5875 0.0 0.0 0.0 0.0 90-91 1.85 0.0 0.0 0.0 0.0 92-93 2.3375 0.0 0.0 0.0 0.0 94-95 2.825 0.0 0.0 0.0 0.0 96-97 3.2 0.0 0.0 0.0 0.0 98-99 3.825 0.0 0.0 0.0 0.0 100-101 4.625 0.0 0.0 0.0 0.0 102-103 5.025 0.0 0.0 0.0 0.0 104-105 5.5 0.0 0.0 0.0 0.0 106-107 6.2125 0.0 0.0 0.0 0.0 108-109 6.9 0.0 0.0 0.0 0.0 110-111 7.574999999999999 0.0 0.0 0.0 0.0 112-113 8.5125 0.0 0.0 0.0 0.0 114-115 9.475 0.0 0.0 0.0 0.0 116-117 10.575 0.0 0.0 0.0 0.0 118-119 11.3875 0.0 0.0 0.0 0.0 120-121 12.05 0.0 0.0 0.0 0.0 122-123 12.725 0.0 0.0 0.0 0.0 124-125 13.7125 0.0 0.0 0.0 0.0 126-127 14.6875 0.0 0.0 0.0 0.0 128-129 15.425 0.0 0.0 0.0 0.0 130-131 16.0375 0.0 0.0 0.0 0.0 132-133 16.8625 0.0 0.0 0.0 0.0 134-135 17.512500000000003 0.0 0.0 0.0 0.0 136-137 18.275 0.0 0.0 0.0 0.0 138-139 18.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGG 10 0.006830828 145.0 9 ACACTGC 10 0.006830828 145.0 7 GCGAAAA 10 0.006830828 145.0 5 ACATTGC 10 0.006830828 145.0 8 TAACATT 10 0.006830828 145.0 6 CTTAACA 10 0.006830828 145.0 4 TTAACAT 10 0.006830828 145.0 5 AACATTG 10 0.006830828 145.0 7 GAGATAG 10 0.006830828 145.0 3 GTGCTTA 10 0.006830828 145.0 1 GCTTAAC 10 0.006830828 145.0 3 CACACAC 20 0.00593511 29.0 10-14 >>END_MODULE Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1726007 spots for SRR13165378.sra Written 1726007 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra Read 1725998 spots for SRR13165378.sra Written 1725998 spots for SRR13165378.sra SRR ids: ['SRR13165378.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6se0n2rj SRR13165378.sra spots: 34519969 blocks: [[1, 1725998], [1725999, 3451996], [3451997, 5177994], [5177995, 6903992], [6903993, 8629990], [8629991, 10355988], [10355989, 12081986], [12081987, 13807984], [13807985, 15533982], [15533983, 17259980], [17259981, 18985978], [18985979, 20711976], [20711977, 22437974], [22437975, 24163972], [24163973, 25889970], [25889971, 27615968], [27615969, 29341966], [29341967, 31067964], [31067965, 32793962], [32793963, 34519969]] SRR13165378 file size 11709695 SRR13165378 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165378 SRR13165378_1.fastq SRR13165378_2.fastq Input file: SRR13165378_1.fastq Paired file: SRR13165378_2.fastq trimmed: SRR13165378-trimmed-pair1.fastq, SRR13165378-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:58:12 2024 >> started Sat Dec 7 17:03:14 2024 >> done (301.756s) 34519969 read pairs processed; of these: 918 ( 0.00%) short read pairs filtered out after trimming by size control 29997 ( 0.09%) empty read pairs filtered out after trimming by size control 34489054 (99.91%) read pairs available; of these: 8206839 (23.80%) trimmed read pairs available after processing 26282215 (76.20%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 49 0.00% 19 38 0.00% 20 60 0.00% 21 78 0.00% 22 105 0.00% 23 91 0.00% 24 111 0.00% 25 131 0.00% 26 115 0.00% 27 136 0.00% 28 179 0.00% 29 185 0.00% 30 260 0.00% 31 213 0.00% 32 240 0.00% 33 234 0.00% 34 210 0.00% 35 277 0.00% 36 290 0.00% 37 298 0.00% 38 302 0.00% 39 328 0.00% 40 406 0.00% 41 445 0.00% 42 522 0.00% 43 494 0.00% 44 600 0.00% 45 611 0.00% 46 568 0.00% 47 822 0.00% 48 929 0.00% 49 1078 0.00% 50 1103 0.00% 51 1262 0.00% 52 1480 0.00% 53 1578 0.00% 54 1683 0.00% 55 1929 0.01% 56 2114 0.01% 57 2343 0.01% 58 2629 0.01% 59 3029 0.01% 60 3439 0.01% 61 4122 0.01% 62 4326 0.01% 63 5095 0.01% 64 5580 0.02% 65 6243 0.02% 66 6727 0.02% 67 7347 0.02% 68 8326 0.02% 69 9104 0.03% 70 10452 0.03% 71 11763 0.03% 72 13352 0.04% 73 15126 0.04% 74 17232 0.05% 75 18529 0.05% 76 20673 0.06% 77 22164 0.06% 78 24012 0.07% 79 25685 0.07% 80 28647 0.08% 81 31460 0.09% 82 34425 0.10% 83 38162 0.11% 84 42902 0.12% 85 45642 0.13% 86 48703 0.14% 87 51916 0.15% 88 54741 0.16% 89 56716 0.16% 90 61418 0.18% 91 64335 0.19% 92 68171 0.20% 93 72450 0.21% 94 77340 0.22% 95 80298 0.23% 96 85324 0.25% 97 87872 0.25% 98 90166 0.26% 99 93033 0.27% 100 95157 0.28% 101 96816 0.28% 102 99212 0.29% 103 104203 0.30% 104 105927 0.31% 105 110321 0.32% 106 113235 0.33% 107 114805 0.33% 108 118745 0.34% 109 119547 0.35% 110 120247 0.35% 111 122514 0.36% 112 122787 0.36% 113 123421 0.36% 114 126680 0.37% 115 128783 0.37% 116 130432 0.38% 117 133604 0.39% 118 134629 0.39% 119 133989 0.39% 120 135416 0.39% 121 136004 0.39% 122 134336 0.39% 123 135422 0.39% 124 138555 0.40% 125 137967 0.40% 126 140041 0.41% 127 138699 0.40% 128 139584 0.40% 129 141689 0.41% 130 139905 0.41% 131 139767 0.41% 132 142081 0.41% 133 141098 0.41% 134 141241 0.41% 135 142640 0.41% 136 141050 0.41% 137 142072 0.41% 138 142306 0.41% 139 143044 0.41% 140 142695 0.41% 141 143699 0.42% 142 143515 0.42% 143 142892 0.41% 144 144724 0.42% 145 144246 0.42% 146 142714 0.41% 147 142783 0.41% 148 142379 0.41% 149 143233 0.42% 150 143415 0.42% 151 26282215 76.20% 34489054 reads passed initial QC criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=5.95 fanout-score-rank=23 prefix-density=0.33 prefix-fanout=4.1 sequence=TGCAGTTGTCGC criterion=fanout-score sequence-density=0.04 sequence-density-rank=39 fanout-score=232.92 fanout-score-rank=1 prefix-density=0.48 prefix-fanout=17.7 sequence=CGCCGCCGCCACCACGGGACTGCGCTTCGTTGACGGTGATGTTGCGGCCATCCAGGTCCTTGCCGTTCATGCCCTCGATGGCGTCGCGCATCGACTGCTCGCTGGCGAAGGTGACGAAGCCGAACCCGCGGGAACGGCCAGTCTCCCTGTCGTTGATGATCTTGGAGTCGATGATCTCGCCGAAGGAGGAGAAGGCATCCTGGAGACCACGGTCGTCGGTAGCCCAGGCGAGGCCGCCCACGAAGCAACGGTACTCAACTTCCGCCATTCCTCCCACTAAACCCTAACGAACCGGAACC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=39 prefix-density=0.44 prefix-fanout=2.1 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.10 sequence-density-rank=27 fanout-score=119.94 fanout-score-rank=1 prefix-density=0.76 prefix-fanout=16.5 sequence=GAAGAAGAAGAAAATGAGCCCCAAAAAAGTTGACAGAGCTTCCATTGTTGTTCTCCTGCTCATCGTGCTTTCGGTTTGTGCCGCAGGAGGAAGGGAGCTGGCGGAACAAAAGCTACAAAAGGACTTCTACAGTGCTGCATCCAAAGAGGGAGCAACAGTTTCGAGCAACCATCCAAGGAACCTCATGGTTAAGACGAACGACTACGGCCGCTACGACCCTGCTCCAGCTTT SRR13165378 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:09:04 Started mapping on | Dec 07 17:09:04 Finished on | Dec 07 17:36:25 Mapping speed, Million of reads per hour | 75.66 Number of input reads | 34489054 Average input read length | 286 UNIQUE READS: Uniquely mapped reads number | 32371191 Uniquely mapped reads % | 93.86% Average mapped length | 285.31 Number of splices: Total | 30496320 Number of splices: Annotated (sjdb) | 28294316 Number of splices: GT/AG | 30019643 Number of splices: GC/AG | 402107 Number of splices: AT/AC | 16639 Number of splices: Non-canonical | 57931 Mismatch rate per base, % | 0.25% Deletion rate per base | 0.01% Deletion average length | 2.39 Insertion rate per base | 0.01% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 467692 % of reads mapped to multiple loci | 1.36% Number of reads mapped to too many loci | 60205 % of reads mapped to too many loci | 0.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.90% % of reads unmapped: other | 0.71% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1650577 1650577 1650577 N_multimapping 467692 467692 467692 N_noFeature 1549784 31449716 1904337 N_ambiguous 675283 3891 109059 UnstrandedReadsAssigned:30146124 PositiveStrandReadsAssigned:917584 NegativeStrandReadsAssigned:30357795 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=141 echo kmer=137 SRR13165378 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165378-trimmed-pair1.fastq SRR13165378-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 34,489,054 reads, 31,005,602 reads pseudoaligned [quant] estimated average fragment length: 232.867 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,213 rounds 52973 SRR13165378.ke.tsv 35125 SRR13165378.se.tsv 88098 total ==> SRR13165378.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 704.859 0 0 PNS24247 1044 812.133 165.032 10.3892 PNS24249 1928 1696.13 156.255 4.70991 PNS24246 1044 812.133 165.032 10.3892 PNS24248 1044 812.133 165.032 10.3892 PNS24244 1471 1239.13 325.65 13.4361 PNS24243 293 118.566 0 0 KQK14069 1603 1371.13 46620.6 1738.35 KQK14071 474 263.245 784.73 152.405 ==> SRR13165378.se.tsv <== BRADI_1g14170v3 50461 BRADI_1g53295v3 1025 BRADI_1g59795v3 1383 BRADI_1g07683v3 0 BRADI_1g00485v3 6 BRADI_1g20270v3 708 BRADI_1g74790v3 743 BRADI_1g09890v3 0 BRADI_1g77505v3 449 BRADI_1g48960v3 0 SRR13165378 completed mapping pipeline successfully