Starting /dee2/code/volunteer_pipeline.sh SRR13165379 current disk space = 1541553528832 free memory = 1599166184 SRR13165379 SRAfilesize 592df7bb89b3e4a3eb5a3952c78dd26a SRR13165379.sra SRR13165379.sra file validated SRR13165379 is paired end SRR13165379 is conventional basespace SRR13165379 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165379_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5555 37.0 37.0 37.0 37.0 37.0 2 36.19875 37.0 37.0 37.0 37.0 37.0 3 36.5375 37.0 37.0 37.0 37.0 37.0 4 36.514 37.0 37.0 37.0 37.0 37.0 5 36.5365 37.0 37.0 37.0 37.0 37.0 6 36.563 37.0 37.0 37.0 37.0 37.0 7 36.329 37.0 37.0 37.0 37.0 37.0 8 36.5485 37.0 37.0 37.0 37.0 37.0 9 36.511 37.0 37.0 37.0 37.0 37.0 10-14 36.5132 37.0 37.0 37.0 37.0 37.0 15-19 36.4045 37.0 37.0 37.0 37.0 37.0 20-24 36.4351 37.0 37.0 37.0 37.0 37.0 25-29 36.411699999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4043 37.0 37.0 37.0 37.0 37.0 35-39 36.3578 37.0 37.0 37.0 37.0 37.0 40-44 36.3135 37.0 37.0 37.0 37.0 37.0 45-49 36.2902 37.0 37.0 37.0 37.0 37.0 50-54 36.2855 37.0 37.0 37.0 37.0 37.0 55-59 36.2286 37.0 37.0 37.0 37.0 37.0 60-64 36.20270000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.165800000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.1434 37.0 37.0 37.0 37.0 37.0 75-79 36.166999999999994 37.0 37.0 37.0 37.0 37.0 80-84 36.1179 37.0 37.0 37.0 37.0 37.0 85-89 36.11829999999999 37.0 37.0 37.0 37.0 37.0 90-94 36.0613 37.0 37.0 37.0 37.0 37.0 95-99 36.1058 37.0 37.0 37.0 37.0 37.0 100-104 36.0124 37.0 37.0 37.0 37.0 37.0 105-109 36.039 37.0 37.0 37.0 37.0 37.0 110-114 35.992000000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.930499999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.7918 37.0 37.0 37.0 37.0 37.0 125-129 35.7531 37.0 37.0 37.0 37.0 37.0 130-134 35.653400000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.497 37.0 37.0 37.0 37.0 37.0 140-144 35.279799999999994 37.0 37.0 37.0 32.2 37.0 145-149 34.931200000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.7885 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 1.0 22 3.0 23 2.0 24 2.0 25 3.0 26 9.0 27 13.0 28 12.0 29 26.0 30 32.0 31 48.0 32 50.0 33 108.0 34 208.0 35 397.0 36 2696.0 37 388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.775000000000006 9.375 5.55 34.300000000000004 2 22.678077019884217 11.125094387113013 33.40045305814246 32.7963755348603 3 20.1 17.45 27.224999999999998 35.225 4 26.924999999999997 23.35 23.05 26.674999999999997 5 27.975 26.924999999999997 23.974999999999998 21.125 6 24.3 30.275000000000002 21.85 23.575 7 18.7 25.124999999999996 36.25 19.925 8 20.724999999999998 22.85 28.375 28.050000000000004 9 18.95 21.775 32.574999999999996 26.700000000000003 10-14 23.835 26.39 25.130000000000003 24.645 15-19 23.155 25.224999999999998 25.679999999999996 25.94 20-24 24.205 25.900000000000002 24.125 25.77 25-29 24.095 25.1 25.165 25.64 30-34 24.02 24.495 25.990000000000002 25.495 35-39 23.77 25.330000000000002 24.855 26.045 40-44 24.415 24.435000000000002 25.369999999999997 25.779999999999998 45-49 24.09 25.105 25.540000000000003 25.264999999999997 50-54 23.3 24.67 25.345000000000002 26.685 55-59 24.445 25.28 24.09 26.185000000000002 60-64 24.154999999999998 24.865000000000002 24.615000000000002 26.365 65-69 23.775 25.45 24.959999999999997 25.814999999999998 70-74 25.465 24.115000000000002 24.275 26.145000000000003 75-79 24.34 24.709999999999997 25.365 25.585 80-84 24.725 25.064999999999998 24.83 25.380000000000003 85-89 24.0 25.755 24.925 25.319999999999997 90-94 24.875 24.995 25.255 24.875 95-99 24.529999999999998 25.485000000000003 23.775 26.21 100-104 24.845 25.240000000000002 24.610000000000003 25.305 105-109 25.15 25.135 23.985 25.729999999999997 110-114 25.290000000000003 24.995 23.849999999999998 25.865 115-119 24.98 25.040000000000003 23.345 26.634999999999998 120-124 25.005 24.75 24.375 25.869999999999997 125-129 24.665 24.855 24.075 26.405 130-134 25.224999999999998 24.36 24.25 26.165 135-139 24.84 23.59 24.67 26.900000000000002 140-144 25.215 24.485 24.325 25.974999999999998 145-149 25.564999999999998 23.54 24.43 26.465 150-151 25.650000000000002 23.7 24.025 26.625 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 0.5 26 0.0 27 3.0 28 7.0 29 6.5 30 9.0 31 18.5 32 16.0 33 14.5 34 25.0 35 30.5 36 54.5 37 70.5 38 71.0 39 88.0 40 99.5 41 123.5 42 147.0 43 164.0 44 178.5 45 169.5 46 171.5 47 181.0 48 178.5 49 171.0 50 173.0 51 162.5 52 139.0 53 138.5 54 127.5 55 100.5 56 96.0 57 104.0 58 99.5 59 86.5 60 83.0 61 80.0 62 66.5 63 61.5 64 61.0 65 69.5 66 69.0 67 48.5 68 40.5 69 42.0 70 39.5 71 29.5 72 21.0 73 14.5 74 12.0 75 14.0 76 10.5 77 4.5 78 1.0 79 0.5 80 2.0 81 1.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.675 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.45 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8792270531401 54.25 2 16.977225672877847 24.6 3 5.175983436853002 11.25 4 1.8978605935127675 5.5 5 0.759144237405107 2.75 6 0.13802622498274672 0.6 7 0.06901311249137336 0.35000000000000003 8 0.03450655624568668 0.2 9 0.03450655624568668 0.22499999999999998 >10 0.03450655624568668 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTATGCATCTCGTAT 11 0.27499999999999997 TruSeq Adapter, Index 2 (97% over 37bp) GTCAGCGCAGTCGCAGTTGCCGCAGCCGCTCGACATGGTGGCCTTAACTT 9 0.22499999999999998 No Hit ATTTCTAAAGCATTGAAAAATAATAATCGGTCGCAAACATCTTGAGTATA 8 0.2 No Hit GTCCATCTTAATACGGAGCTGTCCTTGGTTGTTTATGCCTTGCCGGATTC 7 0.17500000000000002 No Hit CTTGAGGAGGATGCCCTCGAACAAGACGTTGTTCTGGGCCATGTAGTAGA 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTATGCATCGCGTAT 6 0.15 TruSeq Adapter, Index 2 (97% over 37bp) GGTAATGCGAGATGATGACGTTCATTTCATCGATGAATCCCCTACCACAA 6 0.15 No Hit GGATTCCTCGTTTTGTGGAAGGAAGACCATGCCTACACCTGTATTTGTTC 6 0.15 No Hit CTTGGCCCGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTG 6 0.15 No Hit GCCAAGTTCCCAAGGACCCCCTGCATGCTTGATTGAACTAATTGGGCTAG 5 0.125 No Hit CTTGCTATGGGACTTTCTCCTGTACCTTCGCTTGCTAGGGTTTGGAATGA 5 0.125 No Hit GTCAGCATTGGCACAAAGATCCCGCCGCCACCAACGCCGCCAACGCTCCC 5 0.125 No Hit GTCCAGAACATAACTGAGTCGACATAAGAACTAACATCAAGTTTAGAAGC 5 0.125 No Hit CACGCCCATGACGGCTAGTGGGGAAGCAAACATGGAGATGAGCGATGCCA 5 0.125 No Hit GCATCATCAACGTCGCTTCCACCTTTAAGTAGCCTCTTCGTTGGACCTTG 5 0.125 No Hit GCGCTGCCTCGCAAGCTCCCCATCACCGAGGCAACCGAGGTCACGCTGAC 5 0.125 No Hit CCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCA 5 0.125 No Hit CAGATGTTTTCTGATCCTTTTATATGTGGATTCTGGAATAGACGAAGTCA 5 0.125 No Hit AGAACATTTGCGGTCATGTCAATCTTGGATACGTCGATCAGTATGCGCGG 5 0.125 No Hit CCAGTCATTTGTGCTTGAATAGCACACTCTCTTGCGAGCCTCCTTAATTT 5 0.125 No Hit GCTCGGTGCAGTTCTAGACTAAAATTCCACGAGGGCAGGAATGAAGAGGC 5 0.125 No Hit GGAGGAGCGGCGGCTGAGGGGGAGGCCGGCGGTGGACTTGAGGCCCTGGA 5 0.125 No Hit CCTTTGCATCATTTCTCTTGGCACAACTGTCAGGCATGCTTCCGGTGTTC 5 0.125 No Hit TGTTGTTTCTGCTTCTGTTAAACCCAGGGAAATTCCTTGCCTGGGTAAGG 5 0.125 No Hit CAGTAGAATACGCTGTGCTGCCAGTTGGTGTTGCTACTATGACACCATCT 5 0.125 No Hit GGTACTTCTTCTTGATGCGCTCCCGCCGGTGGTATTTCGGGTGCGGGGCC 5 0.125 No Hit ATTCGATTCGTTCGTGAGGAAAGGGATGAAGGGAGACGAAGGGATTGGGA 5 0.125 No Hit CCGGTGATCGTGTTCAACAGCTCGCCCAAGTTATCAAACATTTCATGATA 5 0.125 No Hit GTCACATCAAAAAATGCTATTTCCTGGTTTATGAGATGACTAAATAAGTC 5 0.125 No Hit CTCGTGCACCTCATGCTTAATTACATTGCGCGGGGTTCACTCCACCATGG 5 0.125 No Hit CCGTAGAACATTGAAAGCCTTCTCAAGAATCAGTGACGATCTCTGTACGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0375 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.0875 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.2 0.0 0.0 0.0 0.0 58-59 0.21250000000000002 0.0 0.0 0.0 0.0 60-61 0.2875 0.0 0.0 0.0 0.0 62-63 0.4125 0.0 0.0 0.0 0.0 64-65 0.575 0.0 0.0 0.0 0.0 66-67 0.675 0.0 0.0 0.0 0.0 68-69 0.825 0.0 0.0 0.0 0.0 70-71 0.975 0.0 0.0 0.0 0.0 72-73 1.1749999999999998 0.0 0.0 0.0 0.0 74-75 1.475 0.0 0.0 0.0 0.0 76-77 1.775 0.0 0.0 0.0 0.0 78-79 2.1624999999999996 0.0 0.0 0.0 0.0 80-81 2.675 0.0 0.0 0.0 0.0 82-83 3.175 0.0 0.0 0.0 0.0 84-85 3.8125 0.0 0.0 0.0 0.0 86-87 4.4 0.0 0.0 0.0 0.0 88-89 4.975 0.0 0.0 0.0 0.0 90-91 5.625 0.0 0.0 0.0 0.0 92-93 6.4 0.0 0.0 0.0 0.0 94-95 7.425 0.0 0.0 0.0 0.0 96-97 8.3625 0.0 0.0 0.0 0.0 98-99 9.175 0.0 0.0 0.0 0.0 100-101 9.837499999999999 0.0 0.0 0.0 0.0 102-103 10.7 0.0 0.0 0.0 0.0 104-105 11.412500000000001 0.0 0.0 0.0 0.0 106-107 12.5625 0.0 0.0 0.0 0.0 108-109 13.45 0.0 0.0 0.0 0.0 110-111 14.325 0.0 0.0 0.0 0.0 112-113 15.3 0.0 0.0 0.0 0.0 114-115 16.3125 0.0 0.0 0.0 0.0 116-117 17.4375 0.0 0.0 0.0 0.0 118-119 18.35 0.0 0.0 0.0 0.0 120-121 19.262500000000003 0.0 0.0 0.0 0.0 122-123 20.0375 0.0 0.0 0.0 0.0 124-125 21.012500000000003 0.0 0.0 0.0 0.0 126-127 22.225 0.0 0.0 0.0 0.0 128-129 23.2625 0.0 0.0 0.0 0.0 130-131 24.200000000000003 0.0 0.0 0.0 0.0 132-133 25.1875 0.0 0.0 0.0 0.0 134-135 26.575 0.0 0.0 0.0 0.0 136-137 27.387500000000003 0.0 0.0 0.0 0.0 138-139 28.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCTGG 10 0.006830828 145.0 8 ACCTGGT 10 0.006830828 145.0 9 TGGGGGA 10 0.006830828 145.0 2 GGGAACC 10 0.006830828 145.0 5 TTGTGAC 10 0.006830828 145.0 145 GGAACCT 10 0.006830828 145.0 6 CGATCTC 10 0.006830828 145.0 145 GGGGGGG 80 0.0020131238 18.125 145 >>END_MODULE SRR13165379 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165379_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0575 37.0 37.0 37.0 37.0 37.0 2 36.0145 37.0 37.0 37.0 37.0 37.0 3 36.046 37.0 37.0 37.0 37.0 37.0 4 36.188 37.0 37.0 37.0 37.0 37.0 5 36.236 37.0 37.0 37.0 37.0 37.0 6 36.145 37.0 37.0 37.0 37.0 37.0 7 36.2 37.0 37.0 37.0 37.0 37.0 8 36.1235 37.0 37.0 37.0 37.0 37.0 9 36.15 37.0 37.0 37.0 37.0 37.0 10-14 36.1127 37.0 37.0 37.0 37.0 37.0 15-19 36.0578 37.0 37.0 37.0 37.0 37.0 20-24 36.037850000000006 37.0 37.0 37.0 37.0 37.0 25-29 35.936249999999994 37.0 37.0 37.0 37.0 37.0 30-34 35.91605 37.0 37.0 37.0 37.0 37.0 35-39 35.88055 37.0 37.0 37.0 37.0 37.0 40-44 35.93915 37.0 37.0 37.0 37.0 37.0 45-49 35.869099999999996 37.0 37.0 37.0 37.0 37.0 50-54 35.84845 37.0 37.0 37.0 37.0 37.0 55-59 35.872749999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.849450000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.8249 37.0 37.0 37.0 37.0 37.0 70-74 35.722750000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.7014 37.0 37.0 37.0 37.0 37.0 80-84 35.7082 37.0 37.0 37.0 37.0 37.0 85-89 35.65415 37.0 37.0 37.0 37.0 37.0 90-94 35.5987 37.0 37.0 37.0 37.0 37.0 95-99 35.59805 37.0 37.0 37.0 37.0 37.0 100-104 35.432249999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.35305 37.0 37.0 37.0 37.0 37.0 110-114 35.139149999999994 37.0 37.0 37.0 29.8 37.0 115-119 34.91615 37.0 37.0 37.0 25.0 37.0 120-124 34.622550000000004 37.0 37.0 37.0 25.0 37.0 125-129 34.3461 37.0 37.0 37.0 25.0 37.0 130-134 33.87645 37.0 37.0 37.0 25.0 37.0 135-139 33.69095 37.0 37.0 37.0 16.6 37.0 140-144 33.24885 37.0 37.0 37.0 11.0 37.0 145-149 32.96475 37.0 37.0 37.0 11.0 37.0 150-151 32.70275 37.0 37.0 37.0 11.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 8.0 15 2.0 16 4.0 17 2.0 18 5.0 19 5.0 20 7.0 21 7.0 22 7.0 23 13.0 24 16.0 25 12.0 26 8.0 27 13.0 28 19.0 29 36.0 30 69.0 31 79.0 32 135.0 33 235.0 34 238.0 35 556.0 36 2285.0 37 235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.43847312908087 18.533400301356103 8.83977900552486 25.18834756403817 2 30.625000000000004 20.225 26.85 22.3 3 24.224999999999998 23.125 28.875 23.775 4 26.35 30.225 20.4 23.025000000000002 5 27.075 32.5 18.75 21.675 6 25.424999999999997 33.725 19.625 21.224999999999998 7 23.05 20.65 32.925 23.375 8 25.0 22.75 23.474999999999998 28.775000000000002 9 25.4 22.075 24.875 27.650000000000002 10-14 26.645000000000003 25.369999999999997 23.395 24.59 15-19 26.14 25.165 23.965 24.73 20-24 26.161308065403272 25.711285564278214 24.206210310515523 23.92119605980299 25-29 25.736434108527135 24.896224056014006 24.141035258814703 25.22630657664416 30-34 26.003900585087763 25.358803820573083 23.603540531079663 25.033755063259488 35-39 26.216310815540776 24.946247312365617 23.69618480924046 25.141257062853146 40-44 25.923888583287493 25.15877381607241 23.603540531079663 25.313797069560433 45-49 25.987598759875986 26.037603760376037 23.2973297329733 24.677467746774678 50-54 25.67128356417821 26.036301815090756 23.711185559277965 24.581229061453072 55-59 26.571642910727682 24.79619904976244 23.470867716929234 25.161290322580644 60-64 26.3913195659783 25.591279563978198 23.51617580879044 24.501225061253063 65-69 26.985 25.095 23.705000000000002 24.215 70-74 25.96149037259315 25.171292823205803 24.251062765691422 24.616154038509627 75-79 26.875375075015 24.98499699939988 23.56971394278856 24.56991398279656 80-84 27.21 24.83 23.79 24.169999999999998 85-89 26.691672918229557 24.991247811952988 24.33108277069267 23.98599649912478 90-94 27.202720272027204 25.357535753575355 23.097309730973098 24.342434243424343 95-99 28.15640782039102 24.921246062303116 23.311165558277914 23.611180559027954 100-104 28.56714178544636 25.63640910227557 23.115778944736185 22.680670167541887 105-109 28.982245561390346 25.371342835708926 22.99574893723431 22.650662665666417 110-114 29.486474323716184 25.571278563928196 23.04115205760288 21.901095054752737 115-119 29.76244061015254 26.121530382595648 22.290572643160793 21.82545636409102 120-124 30.65266316579145 24.8062015503876 22.870717679419855 21.670417604401102 125-129 31.681336267253453 24.804960992198442 23.114622924584918 20.39907981596319 130-134 32.44311077769442 24.426106526631656 22.91572893223306 20.21505376344086 135-139 33.633408352088026 23.44586146536634 22.80570142535634 20.115028757189297 140-144 34.53172658632931 23.651182559127957 22.79113955697785 19.025951297564877 145-149 36.12403100775194 23.0207551887972 22.165541385346337 18.689672418104525 150-151 36.25906476619154 23.13078269567392 22.31807951987997 18.292073018254566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 1.5 10 1.5 11 1.0 12 0.5 13 0.5 14 1.0 15 0.5 16 0.0 17 0.5 18 0.5 19 1.5 20 2.0 21 1.0 22 1.5 23 2.0 24 1.5 25 2.5 26 4.5 27 6.5 28 6.0 29 5.0 30 8.0 31 9.0 32 13.0 33 20.5 34 31.5 35 41.0 36 37.5 37 36.5 38 68.0 39 96.5 40 101.5 41 111.5 42 135.0 43 155.5 44 172.5 45 162.5 46 148.0 47 169.5 48 186.5 49 175.0 50 136.0 51 131.5 52 132.0 53 122.5 54 129.0 55 115.0 56 93.5 57 101.0 58 103.5 59 87.5 60 81.0 61 83.0 62 89.0 63 73.0 64 61.0 65 71.0 66 75.0 67 60.0 68 51.5 69 62.0 70 51.0 71 32.5 72 28.0 73 26.5 74 20.5 75 11.0 76 6.5 77 4.5 78 3.5 79 1.0 80 0.0 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 1.5 93 1.5 94 1.0 95 1.0 96 2.0 97 4.0 98 4.0 99 3.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44999999999999996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.02 80-84 0.0 85-89 0.025 90-94 0.01 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.025 135-139 0.025 140-144 0.005 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.45 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14023144996597 55.925000000000004 2 16.33764465622873 24.0 3 4.4928522804629 9.9 4 2.042205582028591 6.0 5 0.7488087134104833 2.75 6 0.06807351940095302 0.3 7 0.03403675970047651 0.17500000000000002 8 0.06807351940095302 0.4 9 0.03403675970047651 0.22499999999999998 >10 0.03403675970047651 0.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 13 0.325 No Hit CACACAGGCAAAACACAGCTGATTCGTGTACTCGATCTCCCCAGCAAGTT 9 0.22499999999999998 No Hit CTACATTATATTTATGCTATTATTTTATGAGCTTTTTACTAGTCAAAGGT 8 0.2 No Hit CTTTACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGT 8 0.2 No Hit GCCTCTTCTCGCTTGCTCTACCTGCTGCTTGCAACCATGGCACCCACCGT 7 0.17500000000000002 No Hit GCGCACGACGCGTTCGTTGGTAACTGGAAGCCCACCAAGAACGACACGCT 6 0.15 No Hit GCAGGATTGATGAGTGGCATACCATGGGACTTGTTTAATGATTGGGCCAG 6 0.15 No Hit GAGATGTTCAGCAGCCAGAAGACAAGGCTCAAGCCCTGGCAGATGTGAAC 5 0.125 No Hit GCTAAAGGTTACAGCAGTGTGAAGGATTTCAAGGAGTTTGCTGATGCTGT 5 0.125 No Hit AGCAGCTAGCTGTAGTATATATCGGTCCATCCATCGGACGATCGATATAT 5 0.125 No Hit GGATGGAAATCCTGTGCGGTCACTACTTTAGGAAGGGGTGGTAGTGACTT 5 0.125 No Hit TGTGCCACAGTATGATCCCTCTTGGTTCTTGCACCATGAAACTAAACGCT 5 0.125 No Hit ATACAGCATATCTGAGTATGAAAATTTAGCTTTGAAGAAGATCAATTCAG 5 0.125 No Hit ATTCAACGGCACCTTTCTGCTGCTCATATTCTTTGACTTCGTCTATTCCA 5 0.125 No Hit TGTTTGAGCGTATTTGGGGTTTTCTAAACCTTGCCTGTTCTCTGTTCCAG 5 0.125 No Hit GGAAGATGATGAGGTTATTGTAAGAGGAATCCGCGTGCCGCCTTCTGTCC 5 0.125 No Hit CCGCCGCGCTGCAGGAGAGGAAGAAGCTGGTGAAGGGGTCGTTCAGGGAC 5 0.125 No Hit GGTATCCTTCTCAACTACTTCTCCCTGTCTAAATTAAATCAAATTAGAAA 5 0.125 No Hit GTTAATTTTTGACCTCCATCAGATCAATCCTAAAGCGAAGGTGTCGGTAA 5 0.125 No Hit CCCAAGATGATCTACGACTACTACGCCTCCGGCGCCGAGGATGAGTGGAC 5 0.125 No Hit GTCAACGGCACCGGAGCGGTGTTCCAGCTCGCTTACATTTCCCTCTTCTT 5 0.125 No Hit CAGCTCAATAACCTTATCCCCTCCCTCCTCGAGAAGAAGCAGTAAGACAC 5 0.125 No Hit CTTGAGAGTAAAAGATGGAATTCCTGACCTACGCCGTGCAGCTAGATCTA 5 0.125 No Hit GTTTGCAAGAGTGCACGTCAGCTTCTTCAGTGTCTTATGGCGTTTATAGA 5 0.125 No Hit CCTTGCGCAAGCCACAAGGCCGAACGAAGAGACCAACCCAGCTGAACCCG 5 0.125 No Hit GGGCACATGGCGGCAGCAATAATAACTACACCTCGCTGCTCCATCATCAT 5 0.125 No Hit GAGGCAGCCTACTACCAGCAAGGCGCCCGCTTCGCCAAGTGGCGCACTGT 5 0.125 No Hit TCTAGGATTCTTGACCTCACATAATTTTGAAGGTTTCAGACAAGACATGA 5 0.125 No Hit GGGAACAAAATCAAAGGAGTATTTTGACCGCCATGGAGATCTTAAGCGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.0625 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.1 0.0 0.0 0.0 0.0 50-51 0.1 0.0 0.0 0.0 0.0 52-53 0.1125 0.0 0.0 0.0 0.0 54-55 0.125 0.0 0.0 0.0 0.0 56-57 0.225 0.0 0.0 0.0 0.0 58-59 0.2375 0.0 0.0 0.0 0.0 60-61 0.3125 0.0 0.0 0.0 0.0 62-63 0.4375 0.0 0.0 0.0 0.0 64-65 0.6 0.0 0.0 0.0 0.0 66-67 0.7 0.0 0.0 0.0 0.0 68-69 0.8500000000000001 0.0 0.0 0.0 0.0 70-71 1.025 0.0 0.0 0.0 0.0 72-73 1.225 0.0 0.0 0.0 0.0 74-75 1.525 0.0 0.0 0.0 0.0 76-77 1.8250000000000002 0.0 0.0 0.0 0.0 78-79 2.2125000000000004 0.0 0.0 0.0 0.0 80-81 2.725 0.0 0.0 0.0 0.0 82-83 3.2249999999999996 0.0 0.0 0.0 0.0 84-85 3.9 0.0 0.0 0.0 0.0 86-87 4.487500000000001 0.0 0.0 0.0 0.0 88-89 5.0375 0.0 0.0 0.0 0.0 90-91 5.7 0.0 0.0 0.0 0.0 92-93 6.4625 0.0 0.0 0.0 0.0 94-95 7.4625 0.0 0.0 0.0 0.0 96-97 8.3625 0.0 0.0 0.0 0.0 98-99 9.1125 0.0 0.0 0.0 0.0 100-101 9.7625 0.0 0.0 0.0 0.0 102-103 10.600000000000001 0.0 0.0 0.0 0.0 104-105 11.337499999999999 0.0 0.0 0.0 0.0 106-107 12.5125 0.0 0.0 0.0 0.0 108-109 13.4125 0.0 0.0 0.0 0.0 110-111 14.274999999999999 0.0 0.0 0.0 0.0 112-113 15.225 0.0 0.0 0.0 0.0 114-115 16.2375 0.0 0.0 0.0 0.0 116-117 17.3625 0.0 0.0 0.0 0.0 118-119 18.2625 0.0 0.0 0.0 0.0 120-121 19.1625 0.0 0.0 0.0 0.0 122-123 19.9375 0.0 0.0 0.0 0.0 124-125 20.924999999999997 0.0 0.0 0.0 0.0 126-127 22.15 0.0 0.0 0.0 0.0 128-129 23.2125 0.0 0.0 0.0 0.0 130-131 24.1625 0.0 0.0 0.0 0.0 132-133 25.15 0.0 0.0 0.0 0.0 134-135 26.5625 0.0 0.0 0.0 0.0 136-137 27.375 0.0 0.0 0.0 0.0 138-139 27.950000000000003 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTTGGC 10 0.006830828 145.0 5 AGCGCCA 10 0.006830828 145.0 5 GGGGGGG 480 3.6379788E-12 12.083333 145 >>END_MODULE Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182263 spots for SRR13165379.sra Written 1182263 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra Read 1182260 spots for SRR13165379.sra Written 1182260 spots for SRR13165379.sra SRR ids: ['SRR13165379.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_pbq1kybh SRR13165379.sra spots: 23645203 blocks: [[1, 1182260], [1182261, 2364520], [2364521, 3546780], [3546781, 4729040], [4729041, 5911300], [5911301, 7093560], [7093561, 8275820], [8275821, 9458080], [9458081, 10640340], [10640341, 11822600], [11822601, 13004860], [13004861, 14187120], [14187121, 15369380], [15369381, 16551640], [16551641, 17733900], [17733901, 18916160], [18916161, 20098420], [20098421, 21280680], [21280681, 22462940], [22462941, 23645203]] SRR13165379 file size 8013974 SRR13165379 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165379 SRR13165379_1.fastq SRR13165379_2.fastq Input file: SRR13165379_1.fastq Paired file: SRR13165379_2.fastq trimmed: SRR13165379-trimmed-pair1.fastq, SRR13165379-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:55:50 2024 >> started Sat Dec 7 16:56:19 2024 >> done (29.390s) 23645203 read pairs processed; of these: 627 ( 0.00%) short read pairs filtered out after trimming by size control 72202 ( 0.31%) empty read pairs filtered out after trimming by size control 23572374 (99.69%) read pairs available; of these: 7692748 (32.63%) trimmed read pairs available after processing 15879626 (67.37%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 22 0.00% 19 39 0.00% 20 39 0.00% 21 34 0.00% 22 61 0.00% 23 70 0.00% 24 90 0.00% 25 96 0.00% 26 114 0.00% 27 160 0.00% 28 190 0.00% 29 189 0.00% 30 271 0.00% 31 241 0.00% 32 283 0.00% 33 314 0.00% 34 350 0.00% 35 355 0.00% 36 449 0.00% 37 486 0.00% 38 596 0.00% 39 647 0.00% 40 744 0.00% 41 904 0.00% 42 948 0.00% 43 1020 0.00% 44 1002 0.00% 45 1155 0.00% 46 1237 0.01% 47 1460 0.01% 48 1780 0.01% 49 2071 0.01% 50 2512 0.01% 51 2928 0.01% 52 3247 0.01% 53 3369 0.01% 54 3655 0.02% 55 3929 0.02% 56 4345 0.02% 57 4811 0.02% 58 5605 0.02% 59 6553 0.03% 60 7343 0.03% 61 8436 0.04% 62 9588 0.04% 63 10749 0.05% 64 11636 0.05% 65 12302 0.05% 66 12957 0.05% 67 14161 0.06% 68 15597 0.07% 69 17289 0.07% 70 19933 0.08% 71 22669 0.10% 72 25227 0.11% 73 28380 0.12% 74 30424 0.13% 75 32978 0.14% 76 34608 0.15% 77 36632 0.16% 78 39298 0.17% 79 42213 0.18% 80 45868 0.19% 81 49963 0.21% 82 54893 0.23% 83 60264 0.26% 84 64094 0.27% 85 67028 0.28% 86 69712 0.30% 87 71390 0.30% 88 73820 0.31% 89 75099 0.32% 90 78979 0.34% 91 82837 0.35% 92 86470 0.37% 93 90385 0.38% 94 95483 0.41% 95 99009 0.42% 96 98924 0.42% 97 101875 0.43% 98 100879 0.43% 99 103127 0.44% 100 103370 0.44% 101 105491 0.45% 102 106642 0.45% 103 107823 0.46% 104 110361 0.47% 105 111567 0.47% 106 111837 0.47% 107 112023 0.48% 108 111423 0.47% 109 112686 0.48% 110 111378 0.47% 111 111711 0.47% 112 112475 0.48% 113 112874 0.48% 114 115290 0.49% 115 115728 0.49% 116 116566 0.49% 117 115714 0.49% 118 115338 0.49% 119 114965 0.49% 120 115037 0.49% 121 112835 0.48% 122 112662 0.48% 123 113552 0.48% 124 115115 0.49% 125 115288 0.49% 126 114118 0.48% 127 113563 0.48% 128 112253 0.48% 129 112697 0.48% 130 111455 0.47% 131 110849 0.47% 132 110649 0.47% 133 110923 0.47% 134 108784 0.46% 135 109399 0.46% 136 109069 0.46% 137 107803 0.46% 138 106709 0.45% 139 106310 0.45% 140 107382 0.46% 141 105717 0.45% 142 106547 0.45% 143 105241 0.45% 144 105731 0.45% 145 105588 0.45% 146 107272 0.46% 147 110434 0.47% 148 104269 0.44% 149 105546 0.45% 150 103799 0.44% 151 15879626 67.37% 23572374 reads passed initial QC criterion=sequence-density sequence-density=0.87 sequence-density-rank=1 fanout-score=2.22 fanout-score-rank=27 prefix-density=0.88 prefix-fanout=2.2 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTCTT criterion=fanout-score sequence-density=0.12 sequence-density-rank=20 fanout-score=58.29 fanout-score-rank=1 prefix-density=0.61 prefix-fanout=11.6 sequence=GGCGGCGGCGGCCTCGCCGTCGCTGGTGTACTTCCCCAGCTGCGCCAGGGAGTTTGCCTTGGCGCGCAGCAGCAGTGCCTCCTGGGCCGCCGCCACGTTCTCCGGCCGTCCTCCCCACGTCTTCAGGCACGTGTTCTGCAGCGCCCTCGCGTATGAGAAGGACACGTGCCACGGGTTCGGCGACTGGTTCATCGCGTTCAGGTTCAGCGTTGCCTCCACCTCTGACTGCCCGCCCGACAGGAACATGATGCCGGGGACGGAAGGAGGGATCCTCCTCTGGAGGAGCTTGAGGG criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=2.98 fanout-score-rank=26 prefix-density=0.52 prefix-fanout=2.5 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.11 sequence-density-rank=15 fanout-score=82.32 fanout-score-rank=1 prefix-density=0.65 prefix-fanout=14.5 sequence=GCCGCCGCCGCCA SRR13165379 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 16:58:05 Started mapping on | Dec 07 16:58:05 Finished on | Dec 07 17:00:24 Mapping speed, Million of reads per hour | 610.51 Number of input reads | 23572374 Average input read length | 278 UNIQUE READS: Uniquely mapped reads number | 21482323 Uniquely mapped reads % | 91.13% Average mapped length | 276.55 Number of splices: Total | 19098365 Number of splices: Annotated (sjdb) | 17820670 Number of splices: GT/AG | 18831250 Number of splices: GC/AG | 225574 Number of splices: AT/AC | 9228 Number of splices: Non-canonical | 32313 Mismatch rate per base, % | 0.22% Deletion rate per base | 0.01% Deletion average length | 2.58 Insertion rate per base | 0.01% Insertion average length | 2.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 511968 % of reads mapped to multiple loci | 2.17% Number of reads mapped to too many loci | 139437 % of reads mapped to too many loci | 0.59% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.17% % of reads unmapped: other | 1.93% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1578317 1578317 1578317 N_multimapping 511968 511968 511968 N_noFeature 931549 20861759 1135458 N_ambiguous 500590 3152 84576 UnstrandedReadsAssigned:20050184 PositiveStrandReadsAssigned:617412 NegativeStrandReadsAssigned:20262289 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=124 echo kmer=119 SRR13165379 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165379-trimmed-pair1.fastq SRR13165379-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,572,374 reads, 20,668,458 reads pseudoaligned [quant] estimated average fragment length: 209.624 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,174 rounds 52973 SRR13165379.ke.tsv 35125 SRR13165379.se.tsv 88098 total ==> SRR13165379.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 727.932 0 0 PNS24247 1044 835.376 56.3781 4.63984 PNS24249 1928 1719.38 87.2385 3.48829 PNS24246 1044 835.376 56.3781 4.63984 PNS24248 1044 835.376 56.3781 4.63984 PNS24244 1471 1262.38 131.627 7.16856 PNS24243 293 131.055 1 0.52459 KQK14069 1603 1394.38 182.871 9.01656 KQK14071 474 282.267 6.38777 1.55583 ==> SRR13165379.se.tsv <== BRADI_1g14170v3 218 BRADI_1g53295v3 103 BRADI_1g59795v3 441 BRADI_1g07683v3 0 BRADI_1g00485v3 2 BRADI_1g20270v3 379 BRADI_1g74790v3 850 BRADI_1g09890v3 3 BRADI_1g77505v3 219 BRADI_1g48960v3 0 SRR13165379 completed mapping pipeline successfully