Starting /dee2/code/volunteer_pipeline.sh SRR13165381 current disk space = 1541555892224 free memory = 1602342992 SRR13165381 SRAfilesize c2c29ab0f73c2e3ead29fd926346d30c SRR13165381.sra SRR13165381.sra file validated SRR13165381 is paired end SRR13165381 is conventional basespace SRR13165381 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165381_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5595 37.0 37.0 37.0 37.0 37.0 2 36.31125 37.0 37.0 37.0 37.0 37.0 3 36.416 37.0 37.0 37.0 37.0 37.0 4 36.5625 37.0 37.0 37.0 37.0 37.0 5 36.5025 37.0 37.0 37.0 37.0 37.0 6 36.515 37.0 37.0 37.0 37.0 37.0 7 36.5895 37.0 37.0 37.0 37.0 37.0 8 36.4565 37.0 37.0 37.0 37.0 37.0 9 36.5005 37.0 37.0 37.0 37.0 37.0 10-14 36.6126 37.0 37.0 37.0 37.0 37.0 15-19 36.528600000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.548100000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.4875 37.0 37.0 37.0 37.0 37.0 30-34 36.4667 37.0 37.0 37.0 37.0 37.0 35-39 36.3489 37.0 37.0 37.0 37.0 37.0 40-44 35.753 37.0 37.0 37.0 34.6 37.0 45-49 33.5827 37.0 31.8 37.0 24.6 37.0 50-54 35.735400000000006 37.0 37.0 37.0 34.6 37.0 55-59 33.32600000000001 37.0 34.6 37.0 19.4 37.0 60-64 32.4381 37.0 29.8 37.0 16.2 37.0 65-69 30.608099999999997 37.0 21.8 37.0 16.2 37.0 70-74 32.599900000000005 37.0 29.4 37.0 19.4 37.0 75-79 35.968999999999994 37.0 37.0 37.0 37.0 37.0 80-84 36.157300000000006 37.0 37.0 37.0 37.0 37.0 85-89 36.2645 37.0 37.0 37.0 37.0 37.0 90-94 36.2538 37.0 37.0 37.0 37.0 37.0 95-99 36.2295 37.0 37.0 37.0 37.0 37.0 100-104 36.235299999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.257999999999996 37.0 37.0 37.0 37.0 37.0 110-114 36.2547 37.0 37.0 37.0 37.0 37.0 115-119 36.2474 37.0 37.0 37.0 37.0 37.0 120-124 36.181599999999996 37.0 37.0 37.0 37.0 37.0 125-129 36.189099999999996 37.0 37.0 37.0 37.0 37.0 130-134 36.160900000000005 37.0 37.0 37.0 37.0 37.0 135-139 36.0654 37.0 37.0 37.0 37.0 37.0 140-144 36.025099999999995 37.0 37.0 37.0 37.0 37.0 145-149 35.888400000000004 37.0 37.0 37.0 37.0 37.0 150-151 35.71275 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 1.0 20 1.0 21 2.0 22 2.0 23 2.0 24 1.0 25 3.0 26 11.0 27 18.0 28 18.0 29 31.0 30 29.0 31 54.0 32 58.0 33 125.0 34 658.0 35 837.0 36 1872.0 37 275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 64.75 8.425 4.3999999999999995 22.425 2 16.09253205934121 40.98566758863465 19.08473723912497 23.83706311289917 3 13.925 9.225 50.05 26.8 4 16.1 14.149999999999999 15.15 54.6 5 50.949999999999996 16.6 15.625 16.825000000000003 6 50.275000000000006 19.6 14.05 16.075 7 12.3 49.9 25.4 12.4 8 12.875 50.3 19.875 16.950000000000003 9 47.3 14.899999999999999 21.125 16.675 10-14 22.42 30.12 16.665 30.795 15-19 22.384999999999998 23.064999999999998 23.465 31.085 20-24 22.31 29.744999999999997 23.935000000000002 24.01 25-29 22.189999999999998 23.255 22.585 31.97 30-34 15.305 23.544999999999998 29.409999999999997 31.740000000000002 35-39 29.025000000000002 22.900000000000002 23.095 24.98 40-44 16.425 22.58 30.31 30.685000000000002 45-49 24.25 22.6 28.315 24.834999999999997 50-54 29.14 16.265 23.47 31.125000000000004 55-59 15.895000000000001 15.765 36.86 31.480000000000004 60-64 23.549999999999997 16.215 35.29 24.945 65-69 24.275 40.739999999999995 17.14 17.845 70-74 49.33 16.314999999999998 17.095 17.26 75-79 49.575 15.93 16.295 18.2 80-84 50.295 15.365 16.74 17.599999999999998 85-89 49.675000000000004 16.37 16.56 17.395 90-94 49.975 16.545 15.955 17.525 95-99 50.57000000000001 16.235 15.690000000000001 17.505000000000003 100-104 50.89 16.555 15.315000000000001 17.24 105-109 50.865 16.18 15.61 17.345 110-114 50.465 16.49 15.73 17.315 115-119 50.085 16.145 15.9 17.87 120-124 50.895 16.605 15.329999999999998 17.169999999999998 125-129 49.87 16.485 15.604999999999999 18.04 130-134 51.015 15.93 15.445 17.61 135-139 50.575 16.814999999999998 15.534999999999998 17.075000000000003 140-144 50.88 16.48 14.985000000000001 17.655 145-149 50.265 16.155 15.5 18.08 150-151 51.387499999999996 14.975 15.6375 18.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.0 23 1.5 24 1.5 25 0.0 26 0.0 27 2.0 28 4.0 29 5.0 30 3.0 31 4.0 32 9.5 33 9.5 34 10.5 35 19.0 36 22.5 37 30.0 38 39.5 39 41.5 40 50.0 41 69.0 42 90.0 43 96.5 44 106.0 45 118.0 46 120.5 47 127.5 48 114.0 49 104.0 50 120.5 51 112.0 52 96.5 53 98.0 54 107.0 55 103.5 56 86.5 57 77.5 58 72.0 59 68.5 60 65.0 61 59.5 62 45.0 63 38.5 64 70.5 65 362.5 66 556.0 67 339.0 68 127.5 69 46.5 70 27.0 71 24.5 72 24.5 73 17.5 74 10.0 75 9.0 76 8.5 77 9.0 78 7.0 79 4.0 80 3.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.575 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21674370826914 36.125 2 16.38418079096045 15.950000000000001 3 5.803800719054957 8.475000000000001 4 2.2598870056497176 4.3999999999999995 5 0.8731381612737544 2.125 6 0.1027221366204417 0.3 7 0.2054442732408834 0.7000000000000001 8 0.0 0.0 9 0.05136106831022085 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.05136106831022085 11.1 >500 0.05136106831022085 20.599999999999998 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGCTATCTCGTAT 824 20.599999999999998 TruSeq Adapter, Index 3 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGCTATCGCGTAT 444 11.1 TruSeq Adapter, Index 3 (97% over 38bp) GCAATATAACAAACAACTGAGACAACCAACAGAGCCATGTACCAGAACTG 9 0.22499999999999998 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGCTATCTCGTATGCC 7 0.17500000000000002 TruSeq Adapter, Index 3 (97% over 35bp) AGGTCTTGCAGCTTCCTTAGCCTCTTTCTGATCCTTGTTCTTCTTTCCCT 7 0.17500000000000002 No Hit CCCGCCGTTCTCATGAATGATCCTGCATATCTCATCAATGCCTTCTTCAT 7 0.17500000000000002 No Hit GNTCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGCTATCTCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 3 (97% over 36bp) GTCAATGTAGCGCCCAACTCCTAAGGACTGGACACCTGTGTCCTTGTCAC 6 0.15 No Hit ATGTAGTCAGCCAGGGTGCGGCCATCCTCAAGCTGCTTGCCAGCGAAGAT 6 0.15 No Hit TCAACATCTTTATTTGGTTCAAGGCAGAAAGCTTCAGGCCAGTCATATCC 5 0.125 No Hit GGGGTCCACACCCATAATGGAGTATGTTTGGTCAGCAAAAGCAAGAGCTG 5 0.125 No Hit TGCGATGACGATCATAATGTTGAGGAAGTAGTTAGACTGCAGAGCCTTCT 5 0.125 No Hit GCAAGAACCAGTACACAGTCTACAGACAGTAGTAACCCAAGCTCTATAAT 5 0.125 No Hit ATTTGAATCTTTGGAAGAGCCTTTGTTGCTTTCTTCAGGGGAAAGGAAGA 5 0.125 No Hit CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC 5 0.125 No Hit GTGTGGCGTGATGGAACCAGGCTTTGCTAACACCATGCCTCTCTCAATGT 5 0.125 No Hit CATAACTAAACCTGTCTGCTTCAACTGCATAAGACGATTCATTTGTCCCT 5 0.125 No Hit CCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAG 5 0.125 No Hit CCGGTCATCTCCTTCTTGATCTGGCACATCTTCCCTTCTTCGAGTTCGAT 5 0.125 No Hit GAGCTGGAATGTGTTCCGACAGGCAAGGCGAACACTGAGGTCATCATCAT 5 0.125 No Hit ATGCTATCACTCTCTTTGCAAGTTTTGAAGCTTTTCTACGAGGTGGGGCC 5 0.125 No Hit GCTAGACTGGCTGAGCACAACAGGGAAACGTTCCAATGAACAGAAAGCAC 5 0.125 No Hit GTTGTCGAAGCCGATGATGCGGACATAGGCGTCCGGGTACTCCTTCTTGA 5 0.125 No Hit GCTGGCTGCGGGCGTAGCTTGCGTGACGGGAGGAGGATGATGAGGAAATG 5 0.125 No Hit ACCTTGTTGTGCTCCTTTACCGTAGAAGGAACCAGTCTTCATGGCATCTG 5 0.125 No Hit CAGAATTATAAATGACCAGACTTTACAAATGACAAAAGGGTCAAAGAGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.0625 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.075 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.0875 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.1375 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.2 0.0 0.0 0.0 0.0 64-65 0.21250000000000002 0.0 0.0 0.0 0.0 66-67 0.2625 0.0 0.0 0.0 0.0 68-69 0.3125 0.0 0.0 0.0 0.0 70-71 0.4 0.0 0.0 0.0 0.0 72-73 0.425 0.0 0.0 0.0 0.0 74-75 0.4375 0.0 0.0 0.0 0.0 76-77 0.575 0.0 0.0 0.0 0.0 78-79 0.625 0.0 0.0 0.0 0.0 80-81 0.8375 0.0 0.0 0.0 0.0 82-83 0.9625 0.0 0.0 0.0 0.0 84-85 1.125 0.0 0.0 0.0 0.0 86-87 1.35 0.0 0.0 0.0 0.0 88-89 1.55 0.0 0.0 0.0 0.0 90-91 1.8125 0.0 0.0 0.0 0.0 92-93 2.125 0.0 0.0 0.0 0.0 94-95 2.3499999999999996 0.0 0.0 0.0 0.0 96-97 2.6875 0.0 0.0 0.0 0.0 98-99 2.9749999999999996 0.0 0.0 0.0 0.0 100-101 3.3375 0.0 0.0 0.0 0.0 102-103 3.9875000000000003 0.0 0.0 0.0 0.0 104-105 4.5375 0.0 0.0 0.0 0.0 106-107 5.012499999999999 0.0 0.0 0.0 0.0 108-109 5.475 0.0 0.0 0.0 0.0 110-111 6.2125 0.0 0.0 0.0 0.0 112-113 6.800000000000001 0.0 0.0 0.0 0.0 114-115 7.5125 0.0 0.0 0.0 0.0 116-117 8.1 0.0 0.0 0.0 0.0 118-119 8.5375 0.0 0.0 0.0 0.0 120-121 8.95 0.0 0.0 0.0 0.0 122-123 9.625 0.0 0.0 0.0 0.0 124-125 10.524999999999999 0.0 0.0 0.0 0.0 126-127 11.45 0.0 0.0 0.0 0.0 128-129 12.1 0.0 0.0 0.0 0.0 130-131 12.649999999999999 0.0 0.0 0.0 0.0 132-133 13.3125 0.0 0.0 0.0 0.0 134-135 14.375 0.0 0.0 0.0 0.0 136-137 15.3 0.0 0.0 0.0 0.0 138-139 16.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCACA 160 0.0 99.6875 9 AAGAGCA 165 0.0 96.66667 7 GAAGAGC 165 0.0 96.66667 6 CGGAAGA 165 0.0 96.66667 4 AGAGCAC 165 0.0 96.66667 8 GGAAGAG 165 0.0 96.66667 5 TCGGAAG 170 0.0 93.82353 3 ATCGGAA 170 0.0 93.82353 2 GATCGGA 175 0.0 91.14286 1 AGGGGGG 85 0.0 29.0 65-69 GAAATGG 20 0.00593511 29.0 65-69 TCGCGTA 40 2.9937655E-7 29.0 40-44 TATCGCG 40 2.9937655E-7 29.0 40-44 AAGGGGG 80 0.0 29.0 65-69 AAAGGGG 80 0.0 29.0 65-69 AATGGGG 20 0.00593511 29.0 65-69 CTGCTTG 100 0.0 29.0 55-59 GCGTATG 40 2.9937655E-7 29.0 45-49 ATCGCGT 40 2.9937655E-7 29.0 40-44 CGCGTAT 40 2.9937655E-7 29.0 40-44 >>END_MODULE SRR13165381 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165381_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 66 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.07075 37.0 37.0 37.0 37.0 37.0 2 35.8295 37.0 37.0 37.0 37.0 37.0 3 35.5465 37.0 37.0 37.0 37.0 37.0 4 35.382 37.0 37.0 37.0 37.0 37.0 5 35.719 37.0 37.0 37.0 37.0 37.0 6 35.881 37.0 37.0 37.0 37.0 37.0 7 35.0045 37.0 37.0 37.0 25.0 37.0 8 33.794 37.0 37.0 37.0 25.0 37.0 9 33.8785 37.0 37.0 37.0 25.0 37.0 10-14 33.1169 37.0 37.0 37.0 25.0 37.0 15-19 33.04600000000001 37.0 34.6 37.0 22.2 37.0 20-24 32.64445 37.0 32.2 37.0 16.6 37.0 25-29 29.872450000000004 37.0 25.0 37.0 11.0 37.0 30-34 28.933049999999998 37.0 16.6 37.0 11.0 37.0 35-39 28.10485 37.0 13.8 37.0 11.0 37.0 40-44 28.97565 37.0 19.4 37.0 11.0 37.0 45-49 27.603950000000005 37.0 11.0 37.0 11.0 37.0 50-54 28.096749999999997 37.0 11.0 37.0 11.0 37.0 55-59 29.937149999999995 37.0 25.0 37.0 11.0 37.0 60-64 31.17685 37.0 25.0 37.0 11.0 37.0 65-69 29.557 37.0 22.2 37.0 11.0 37.0 70-74 27.77035 37.0 11.0 37.0 11.0 37.0 75-79 27.913549999999997 37.0 11.0 37.0 11.0 37.0 80-84 30.634950000000003 37.0 25.0 37.0 11.0 37.0 85-89 33.005849999999995 37.0 37.0 37.0 13.8 37.0 90-94 34.094550000000005 37.0 37.0 37.0 25.0 37.0 95-99 34.55565 37.0 37.0 37.0 25.0 37.0 100-104 34.70315000000001 37.0 37.0 37.0 25.0 37.0 105-109 34.84235 37.0 37.0 37.0 25.0 37.0 110-114 34.91455 37.0 37.0 37.0 25.0 37.0 115-119 34.97285 37.0 37.0 37.0 27.4 37.0 120-124 34.917950000000005 37.0 37.0 37.0 25.0 37.0 125-129 34.94615 37.0 37.0 37.0 27.4 37.0 130-134 34.78085 37.0 37.0 37.0 25.0 37.0 135-139 34.8778 37.0 37.0 37.0 27.4 37.0 140-144 34.77325 37.0 37.0 37.0 25.0 37.0 145-149 34.55305 37.0 37.0 37.0 25.0 37.0 150-151 34.361125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 11.0 13 20.0 14 25.0 15 14.0 16 17.0 17 22.0 18 22.0 19 40.0 20 28.0 21 30.0 22 59.0 23 56.0 24 70.0 25 108.0 26 134.0 27 201.0 28 250.0 29 258.0 30 144.0 31 56.0 32 55.0 33 70.0 34 136.0 35 408.0 36 1630.0 37 135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 64.31618569636134 12.622333751568382 6.875784190715183 16.18569636135508 2 57.099999999999994 14.174999999999999 14.174999999999999 14.549999999999999 3 52.87500000000001 16.0 16.475 14.649999999999999 4 56.10000000000001 18.0 12.575 13.325000000000001 5 54.1 19.6 12.275 14.025000000000002 6 52.225 21.875 11.675 14.224999999999998 7 51.849999999999994 13.625000000000002 20.45 14.075 8 51.975 14.025000000000002 14.325 19.675 9 52.775000000000006 13.950000000000001 15.15 18.125 10-14 52.544999999999995 16.465 14.34 16.650000000000002 15-19 52.715 16.215 14.954999999999998 16.115 20-24 52.38309577394349 16.469117279319832 14.678669667416855 16.469117279319832 25-29 51.84296074018505 16.619154788697173 15.243810952738185 16.294073518379594 30-34 46.41660415103776 17.11927981995499 20.795198799699925 15.668917229307327 35-39 43.79594898724682 16.859214803700926 23.42585646411603 15.918979744936234 40-44 48.68217054263566 17.289322330582646 17.86946736684171 16.159039759939986 45-49 36.959239809952486 17.4143535883971 29.457364341085274 16.169042260565142 50-54 43.770942735683924 17.92448112028007 22.510627656914227 15.793948487121781 55-59 50.53263315828958 17.164291072768194 16.114028507126783 16.189047261815453 60-64 51.867966991747934 17.00425106276569 15.103775943985998 16.024006001500375 65-69 50.98019603920784 16.908381676335267 15.733146629325864 16.378275655131024 70-74 46.1565391347837 19.174793698424605 18.469617404351087 16.19904976244061 75-79 44.39109777444361 20.27006751687922 19.33483370842711 16.004001000250064 80-84 50.25756439109777 17.75943985996499 16.239059764941235 15.743935983996 85-89 51.127781945486376 17.349337334333583 15.933983495873969 15.588897224306075 90-94 52.398099524881225 16.864216054013504 15.198799699924981 15.538884721180295 95-99 52.56814203550888 16.454113528382095 15.193798449612403 15.783945986496626 100-104 52.73318329582396 17.164291072768194 14.788697174293574 15.313828457114278 105-109 52.38809702425606 16.35908977244311 15.49887471867967 15.753938484621155 110-114 52.563140785196296 17.099274818704675 14.723680920230056 15.613903475868968 115-119 53.18329582395599 16.22905726431608 14.95373843460865 15.63390847711928 120-124 54.058514628657164 16.664166041510377 14.988747186796699 14.288572143035758 125-129 53.97349337334334 17.16929232308077 14.443610902725682 14.413603400850212 130-134 54.4140449157205 16.910918821587558 14.54509078177362 14.129945480918321 135-139 54.37131139341802 16.77003100930279 15.134540362108634 13.724117235170553 140-144 54.848712178044515 16.44411102775694 14.913728432108028 13.79344836209052 145-149 55.09377344336084 16.43910977744436 14.973743435858966 13.493373343335834 150-151 56.15855945979742 15.243216206077278 15.093159934975617 13.50506439914968 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 0.5 9 0.0 10 0.5 11 0.5 12 0.5 13 2.5 14 2.5 15 1.0 16 1.0 17 1.0 18 1.5 19 2.0 20 1.5 21 1.0 22 1.0 23 1.0 24 1.5 25 2.5 26 3.0 27 2.5 28 2.0 29 4.0 30 5.0 31 4.5 32 5.5 33 7.0 34 12.0 35 16.5 36 19.0 37 26.5 38 39.5 39 41.5 40 50.5 41 67.5 42 84.0 43 89.5 44 88.5 45 94.0 46 109.0 47 107.0 48 105.5 49 118.5 50 115.0 51 103.5 52 89.0 53 90.5 54 96.5 55 95.0 56 76.5 57 71.0 58 88.0 59 80.0 60 61.0 61 44.5 62 41.5 63 49.0 64 49.5 65 44.0 66 31.5 67 42.0 68 57.5 69 53.5 70 41.5 71 29.0 72 22.5 73 24.5 74 21.0 75 19.5 76 15.5 77 9.5 78 15.0 79 14.5 80 17.0 81 20.5 82 20.5 83 23.0 84 21.5 85 20.5 86 24.5 87 28.5 88 28.0 89 31.0 90 37.0 91 56.0 92 78.0 93 102.5 94 130.0 95 130.0 96 122.5 97 112.0 98 91.0 99 73.0 100 73.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.02 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.034999999999999996 135-139 0.03 140-144 0.025 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 78.72869318181817 55.425000000000004 2 12.890625 18.15 3 4.6164772727272725 9.75 4 1.953125 5.5 5 0.9588068181818182 3.375 6 0.17755681818181818 0.75 7 0.14204545454545456 0.7000000000000001 8 0.2130681818181818 1.2 9 0.03551136363636364 0.22499999999999998 >10 0.24857954545454544 2.4250000000000003 >50 0.03551136363636364 2.5 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 100 2.5 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG 25 0.625 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGGGG 16 0.4 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG 15 0.375 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG 11 0.27499999999999997 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGTGG 10 0.25 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 10 0.25 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG 10 0.25 No Hit CTGGACTGTTCCTCCTTGTTCAAGTTGTTCTTTTGTTGGACTTCGTGCAT 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGT 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGTGG 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGTG 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGGGGT 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGTGGGGG 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGTGGGGT 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGTGTTGG 7 0.17500000000000002 No Hit CTACCTACAGCTGCAGCAGGACTCCGTCGCCTGCAGAGTCCGCGCCAAGC 7 0.17500000000000002 No Hit AGCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGT 7 0.17500000000000002 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGTGTGGG 6 0.15 No Hit GGAGTCCACACTCCACTTGGTGCTCAGGCTGCGTGGTGGCATGCAGATCT 6 0.15 No Hit TCTCACTGTGCCACAGTATGATCCCTCTTGGTTCTTGCACCATGAAACTA 6 0.15 No Hit GCCGTCGCCGCTTCCTTGGATGACCGAGGAAGAACTGGGCCAATACGCCG 6 0.15 No Hit AGCTGTCGGACACGATGAAGCAGAAGATCAGGGCGGAGTACGAGGGGCTT 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGTGTTGT 5 0.125 No Hit ATCGAACCGTGGTGCTATGGCTCCAAAGGTGGAACCAGACTATGCCAGTC 5 0.125 No Hit CTTGAGATTCCAGAACTAAAAGCAGCCAAAAACACCTCCGAAGCTAAGGA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTGGGGGGGGGGGGT 5 0.125 No Hit AACACGAATGCTCAATCCCCGCCCTGCAGAACGCTTGACAGCACATGAAG 5 0.125 No Hit GCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGTGTGGT 5 0.125 No Hit TCGTTTGGGGTTCGTCTACCTGACAGCACAGAACAGTCTAGTGGTTCTGT 5 0.125 No Hit TAATTATCGCTTCCACGCCGTTGTTACGGCACCTGCGTCCTGCACGTATG 5 0.125 No Hit GATTGCTTAAATTGGAGGCTTCAGAATGAGATTGACAGTGTTCTTGCTAA 5 0.125 No Hit GATGGTGTTTCCACATTCGAGTTCATTAGAAGTGGAGCAGTTGCAGCATT 5 0.125 No Hit CGTGGATGCCCACATCCCCGTCCCGCAGCGGCAGACCGACCTTCCTTTCT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTGT 5 0.125 No Hit GCATCTTTGTATATGGAGGGGCATGCAGCTTTATGGCTCCAAGCGTTTAA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGTGTG 5 0.125 No Hit GGACAAAGCAAATACTAGGTCACAGGGAACACGAACTTCTGAACTAAAGA 5 0.125 No Hit GGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGA 5 0.125 No Hit GAGGAAAACCCCCGTGTGCCCATCATCGTCACTGGTAACGATTTCTCCAC 5 0.125 No Hit GAGATTTGGAATGCAGAGAGCGTTGATTGTCCATTCAAAGGGGTTGGACG 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGTTGG 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGTTGT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGTGGGGT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGGGGGGGGTGGT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGT 5 0.125 No Hit AGCACAACAGCTCCCCTGGGTACTATGATGGCAGGTACTGGACAATGTGG 5 0.125 No Hit GTCGCCGTCGGCCTCGCCGCGCTTCCGCGTCTACGTCTTCGCGTCGTCGC 5 0.125 No Hit ATGCTATCACGCTTTGCCTTCAGATGATAACAGAGACTGGATTTCATCGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.0625 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.075 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.0875 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.1375 0.0 0.0 0.0 0.0 60-61 0.15 0.0 0.0 0.0 0.0 62-63 0.2 0.0 0.0 0.0 0.0 64-65 0.2 0.0 0.0 0.0 0.0 66-67 0.23750000000000002 0.0 0.0 0.0 0.0 68-69 0.2875 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.4125 0.0 0.0 0.0 0.0 74-75 0.4375 0.0 0.0 0.0 0.0 76-77 0.575 0.0 0.0 0.0 0.0 78-79 0.6125 0.0 0.0 0.0 0.0 80-81 0.8125 0.0 0.0 0.0 0.0 82-83 0.9375 0.0 0.0 0.0 0.0 84-85 1.1125 0.0 0.0 0.0 0.0 86-87 1.3 0.0 0.0 0.0 0.0 88-89 1.5 0.0 0.0 0.0 0.0 90-91 1.7625 0.0 0.0 0.0 0.0 92-93 2.075 0.0 0.0 0.0 0.0 94-95 2.3 0.0 0.0 0.0 0.0 96-97 2.6375 0.0 0.0 0.0 0.0 98-99 2.925 0.0 0.0 0.0 0.0 100-101 3.3125 0.0 0.0 0.0 0.0 102-103 3.9499999999999997 0.0 0.0 0.0 0.0 104-105 4.4875 0.0 0.0 0.0 0.0 106-107 4.975 0.0 0.0 0.0 0.0 108-109 5.4125 0.0 0.0 0.0 0.0 110-111 6.112500000000001 0.0 0.0 0.0 0.0 112-113 6.699999999999999 0.0 0.0 0.0 0.0 114-115 7.4375 0.0 0.0 0.0 0.0 116-117 8.025 0.0 0.0 0.0 0.0 118-119 8.4625 0.0 0.0 0.0 0.0 120-121 8.85 0.0 0.0 0.0 0.0 122-123 9.5 0.0 0.0 0.0 0.0 124-125 10.375 0.0 0.0 0.0 0.0 126-127 11.2875 0.0 0.0 0.0 0.0 128-129 11.9 0.0 0.0 0.0 0.0 130-131 12.45 0.0 0.0 0.0 0.0 132-133 13.087499999999999 0.0 0.0 0.0 0.0 134-135 14.2 0.0 0.0 0.0 0.0 136-137 15.1125 0.0 0.0 0.0 0.0 138-139 15.899999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGCTC 10 0.006830828 145.0 1 GGGTGTT 30 0.0014437955 24.166668 40-44 GGGTTTT 40 2.9585467E-4 21.75 40-44 GGGGTTT 70 3.8434082E-5 16.571428 40-44 GGGGGTT 110 1.3008325E-4 11.863637 40-44 GGGGGGT 260 9.640644E-11 10.596154 25-29 TGGGGGG 265 1.7192597E-6 8.207547 35-39 >>END_MODULE Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra Read 1210601 spots for SRR13165381.sra Written 1210601 spots for SRR13165381.sra Read 1210600 spots for SRR13165381.sra Written 1210600 spots for SRR13165381.sra SRR ids: ['SRR13165381.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_70lii00j SRR13165381.sra spots: 24212001 blocks: [[1, 1210600], [1210601, 2421200], [2421201, 3631800], [3631801, 4842400], [4842401, 6053000], [6053001, 7263600], [7263601, 8474200], [8474201, 9684800], [9684801, 10895400], [10895401, 12106000], [12106001, 13316600], [13316601, 14527200], [14527201, 15737800], [15737801, 16948400], [16948401, 18159000], [18159001, 19369600], [19369601, 20580200], [20580201, 21790800], [21790801, 23001400], [23001401, 24212001]] SRR13165381 file size 8206596 SRR13165381 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165381 SRR13165381_1.fastq SRR13165381_2.fastq Input file: SRR13165381_1.fastq Paired file: SRR13165381_2.fastq trimmed: SRR13165381-trimmed-pair1.fastq, SRR13165381-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 16:58:39 2024 >> started Sat Dec 7 16:59:04 2024 >> done (24.919s) 24212001 read pairs processed; of these: 1096 ( 0.00%) short read pairs filtered out after trimming by size control 7009341 (28.95%) empty read pairs filtered out after trimming by size control 17201564 (71.05%) read pairs available; of these: 4694484 (27.29%) trimmed read pairs available after processing 12507080 (72.71%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 89 0.00% 19 64 0.00% 20 131 0.00% 21 166 0.00% 22 223 0.00% 23 227 0.00% 24 351 0.00% 25 237 0.00% 26 293 0.00% 27 377 0.00% 28 580 0.00% 29 422 0.00% 30 670 0.00% 31 488 0.00% 32 464 0.00% 33 578 0.00% 34 471 0.00% 35 471 0.00% 36 634 0.00% 37 493 0.00% 38 827 0.00% 39 668 0.00% 40 714 0.00% 41 733 0.00% 42 702 0.00% 43 802 0.00% 44 743 0.00% 45 908 0.01% 46 974 0.01% 47 903 0.01% 48 1052 0.01% 49 1160 0.01% 50 1401 0.01% 51 1375 0.01% 52 1537 0.01% 53 1691 0.01% 54 1869 0.01% 55 1739 0.01% 56 2072 0.01% 57 2166 0.01% 58 2311 0.01% 59 2651 0.02% 60 3104 0.02% 61 3383 0.02% 62 3997 0.02% 63 4558 0.03% 64 5094 0.03% 65 4722 0.03% 66 4627 0.03% 67 5178 0.03% 68 6126 0.04% 69 6784 0.04% 70 7529 0.04% 71 8625 0.05% 72 9628 0.06% 73 11686 0.07% 74 11929 0.07% 75 13095 0.08% 76 13691 0.08% 77 15386 0.09% 78 15941 0.09% 79 17985 0.10% 80 19226 0.11% 81 20676 0.12% 82 23709 0.14% 83 25956 0.15% 84 28480 0.17% 85 29228 0.17% 86 32139 0.19% 87 32831 0.19% 88 34158 0.20% 89 35227 0.20% 90 38178 0.22% 91 39472 0.23% 92 41505 0.24% 93 44741 0.26% 94 47341 0.28% 95 49344 0.29% 96 52363 0.30% 97 52303 0.30% 98 52542 0.31% 99 55937 0.33% 100 55806 0.32% 101 57412 0.33% 102 59046 0.34% 103 60463 0.35% 104 61367 0.36% 105 61504 0.36% 106 63877 0.37% 107 64315 0.37% 108 65278 0.38% 109 66406 0.39% 110 66516 0.39% 111 68406 0.40% 112 68880 0.40% 113 69033 0.40% 114 70852 0.41% 115 72368 0.42% 116 73581 0.43% 117 72161 0.42% 118 72482 0.42% 119 71514 0.42% 120 74509 0.43% 121 74118 0.43% 122 74251 0.43% 123 74898 0.44% 124 76559 0.45% 125 76809 0.45% 126 78020 0.45% 127 78604 0.46% 128 77645 0.45% 129 77680 0.45% 130 77095 0.45% 131 77216 0.45% 132 75592 0.44% 133 78628 0.46% 134 77684 0.45% 135 78269 0.46% 136 79437 0.46% 137 77393 0.45% 138 78721 0.46% 139 77659 0.45% 140 77906 0.45% 141 77353 0.45% 142 79164 0.46% 143 79673 0.46% 144 79443 0.46% 145 80710 0.47% 146 78909 0.46% 147 79662 0.46% 148 77410 0.45% 149 78902 0.46% 150 78447 0.46% 151 12507080 72.71% 17201564 reads passed initial QC criterion=sequence-density sequence-density=1.67 sequence-density-rank=1 fanout-score=25.68 fanout-score-rank=1 prefix-density=0.99 prefix-fanout=25.7 sequence=GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAT criterion=fanout-score sequence-density=1.67 sequence-density-rank=1 fanout-score=25.68 fanout-score-rank=1 prefix-density=0.99 prefix-fanout=25.7 sequence=GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGCTATCTCGTATGCCGTCTTCTGCTTGAAAAT criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=2.76 fanout-score-rank=20 prefix-density=0.50 prefix-fanout=2.4 sequence=GCACCAGCTGCACCTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=73.43 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=5.2 sequence=GACCAAGGAGTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGGGCCGCACCGCTGGCCGACCCTGATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAA SRR13165381 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:00:00 Started mapping on | Dec 07 17:00:00 Finished on | Dec 07 17:02:33 Mapping speed, Million of reads per hour | 404.74 Number of input reads | 17201564 Average input read length | 283 UNIQUE READS: Uniquely mapped reads number | 14061884 Uniquely mapped reads % | 81.75% Average mapped length | 282.61 Number of splices: Total | 11552240 Number of splices: Annotated (sjdb) | 10729182 Number of splices: GT/AG | 11385541 Number of splices: GC/AG | 138741 Number of splices: AT/AC | 6037 Number of splices: Non-canonical | 21921 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.66 Insertion rate per base | 0.01% Insertion average length | 2.46 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 733498 % of reads mapped to multiple loci | 4.26% Number of reads mapped to too many loci | 217254 % of reads mapped to too many loci | 1.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.95% % of reads unmapped: other | 4.77% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2406384 2406384 2406384 N_multimapping 733498 733498 733498 N_noFeature 759887 13648176 903502 N_ambiguous 324548 2087 54798 UnstrandedReadsAssigned:12977449 PositiveStrandReadsAssigned:411621 NegativeStrandReadsAssigned:13103584 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=135 echo kmer=131 SRR13165381 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165381-trimmed-pair1.fastq SRR13165381-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,201,564 reads, 13,749,589 reads pseudoaligned [quant] estimated average fragment length: 213.175 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,241 rounds 52973 SRR13165381.ke.tsv 35125 SRR13165381.se.tsv 88098 total ==> SRR13165381.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 724.335 23.8929 3.30065 PNS24247 1044 831.825 59.3293 7.13685 PNS24249 1928 1715.83 101.284 5.90659 PNS24246 1044 831.825 59.3293 7.13685 PNS24248 1044 831.825 59.3293 7.13685 PNS24244 1471 1258.83 78.8353 6.2665 PNS24243 293 120.935 0 0 KQK14069 1603 1390.83 82.3177 5.9223 KQK14071 474 272.51 0 0 ==> SRR13165381.se.tsv <== BRADI_1g14170v3 93 BRADI_1g53295v3 61 BRADI_1g59795v3 241 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 343 BRADI_1g74790v3 692 BRADI_1g09890v3 0 BRADI_1g77505v3 117 BRADI_1g48960v3 0 SRR13165381 completed mapping pipeline successfully