Starting /dee2/code/volunteer_pipeline.sh SRR13165382 current disk space = 1541494243328 free memory = 1597586268 SRR13165382 SRAfilesize 9e24e29e2ace62cc26dce0e72dcaa4ff SRR13165382.sra SRR13165382.sra file validated SRR13165382 is paired end SRR13165382 is conventional basespace SRR13165382 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165382_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.49 37.0 37.0 37.0 37.0 37.0 2 36.0755 37.0 37.0 37.0 37.0 37.0 3 36.416 37.0 37.0 37.0 37.0 37.0 4 36.3505 37.0 37.0 37.0 37.0 37.0 5 36.533 37.0 37.0 37.0 37.0 37.0 6 36.5585 37.0 37.0 37.0 37.0 37.0 7 36.519 37.0 37.0 37.0 37.0 37.0 8 36.4355 37.0 37.0 37.0 37.0 37.0 9 36.6075 37.0 37.0 37.0 37.0 37.0 10-14 36.4914 37.0 37.0 37.0 37.0 37.0 15-19 36.47580000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.4988 37.0 37.0 37.0 37.0 37.0 25-29 36.390499999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.3669 37.0 37.0 37.0 37.0 37.0 35-39 36.3245 37.0 37.0 37.0 37.0 37.0 40-44 36.3731 37.0 37.0 37.0 37.0 37.0 45-49 36.0824 37.0 37.0 37.0 37.0 37.0 50-54 36.1963 37.0 37.0 37.0 37.0 37.0 55-59 36.073 37.0 37.0 37.0 37.0 37.0 60-64 36.054700000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.964600000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.04879999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.184999999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.1255 37.0 37.0 37.0 37.0 37.0 85-89 36.1349 37.0 37.0 37.0 37.0 37.0 90-94 36.0339 37.0 37.0 37.0 37.0 37.0 95-99 36.067099999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.99570000000001 37.0 37.0 37.0 37.0 37.0 105-109 36.039300000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.8872 37.0 37.0 37.0 37.0 37.0 115-119 35.8894 37.0 37.0 37.0 37.0 37.0 120-124 35.770599999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.7304 37.0 37.0 37.0 37.0 37.0 130-134 35.612 37.0 37.0 37.0 37.0 37.0 135-139 35.5305 37.0 37.0 37.0 37.0 37.0 140-144 35.2729 37.0 37.0 37.0 34.6 37.0 145-149 34.9485 37.0 37.0 37.0 27.4 37.0 150-151 34.69725 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 1.0 21 1.0 22 1.0 23 2.0 24 2.0 25 5.0 26 6.0 27 14.0 28 15.0 29 20.0 30 22.0 31 37.0 32 64.0 33 144.0 34 217.0 35 435.0 36 2657.0 37 354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.925000000000004 13.175 4.8500000000000005 24.05 2 25.930583501006037 12.726358148893361 33.5513078470825 27.791750503018108 3 22.650000000000002 25.275 31.674999999999997 20.4 4 27.375 25.900000000000002 23.200000000000003 23.525 5 25.374999999999996 33.225 21.75 19.650000000000002 6 26.275 31.424999999999997 20.7 21.6 7 21.55 22.675 36.225 19.55 8 19.900000000000002 22.075 26.950000000000003 31.075000000000003 9 25.025 19.85 26.700000000000003 28.425 10-14 25.165 24.36 23.799999999999997 26.674999999999997 15-19 25.235000000000003 23.77 24.79 26.205000000000002 20-24 25.45 24.545 24.13 25.874999999999996 25-29 24.990000000000002 23.96 24.404999999999998 26.645000000000003 30-34 25.255 24.095 23.919999999999998 26.729999999999997 35-39 24.92 24.205 24.65 26.224999999999998 40-44 25.64 24.740000000000002 23.7 25.919999999999998 45-49 26.395000000000003 24.505 22.935 26.165 50-54 26.009999999999998 24.465 23.69 25.835 55-59 24.654999999999998 24.215 24.77 26.36 60-64 26.06 23.34 24.555 26.045 65-69 25.509999999999998 24.865000000000002 23.52 26.105 70-74 26.875 24.265 23.225 25.635 75-79 26.75 23.97 23.32 25.96 80-84 26.705000000000002 23.605 23.56 26.13 85-89 25.96 23.35 23.515 27.175 90-94 27.185 23.919999999999998 23.064999999999998 25.83 95-99 27.215 24.175 22.375 26.235000000000003 100-104 27.265 23.665 22.775000000000002 26.295 105-109 27.139999999999997 23.94 23.32 25.6 110-114 27.445000000000004 23.69 22.405 26.46 115-119 27.495000000000005 24.015 22.74 25.75 120-124 27.185 24.59 22.439999999999998 25.785000000000004 125-129 27.529999999999998 23.785 21.834999999999997 26.85 130-134 27.32 23.94 22.405 26.334999999999997 135-139 26.58 23.810000000000002 22.485 27.125 140-144 26.405 23.775 23.24 26.58 145-149 27.084999999999997 23.07 23.155 26.69 150-151 28.675 23.0125 22.1 26.2125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.5 23 0.5 24 0.0 25 0.0 26 1.0 27 3.5 28 4.0 29 4.0 30 7.5 31 9.0 32 8.5 33 19.0 34 28.5 35 25.0 36 33.5 37 55.5 38 58.5 39 72.5 40 102.5 41 113.5 42 127.5 43 148.5 44 150.0 45 171.0 46 181.5 47 149.0 48 138.0 49 141.0 50 142.5 51 148.5 52 145.0 53 122.5 54 104.0 55 106.0 56 109.5 57 100.0 58 84.5 59 83.0 60 91.5 61 79.0 62 69.0 63 83.5 64 81.5 65 90.0 66 100.5 67 84.5 68 67.5 69 54.0 70 56.5 71 49.5 72 39.0 73 41.5 74 32.5 75 22.5 76 18.5 77 10.5 78 7.5 79 7.0 80 4.5 81 2.0 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.6 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.775 #Duplication Level Percentage of deduplicated Percentage of total 1 73.11903920876016 51.74999999999999 2 18.120805369127517 25.650000000000002 3 5.722359590250795 12.15 4 2.013422818791946 5.7 5 0.49452490286117984 1.7500000000000002 6 0.1412928293889085 0.6 7 0.282585658777817 1.4000000000000001 8 0.035323207347227124 0.2 9 0.035323207347227124 0.22499999999999998 >10 0.035323207347227124 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGAGATCTCGTAT 23 0.575 TruSeq Adapter, Index 4 (97% over 38bp) GCAGTCGCGGGCCATGTGGCCGGGCTCGCCGCAGTTGTAGCAGTCCCTGG 9 0.22499999999999998 No Hit CGAAGGCTGTCGCGTTGCAGTAAAAGAATACCTCAAATCGAGCAGCATAG 8 0.2 No Hit GGATGCCAATGTTGGGCTTTGGGCTCTCATAGCTTATTACCTCCCGTCCG 7 0.17500000000000002 No Hit CAGATGTTGAGTGAATCTCTTCAGTGCCGATCAGCAGTTCACGCCACACT 7 0.17500000000000002 No Hit CGGGATCCCACCACACAACACAACACAACAGGCCACCCACCGGTGACGGA 7 0.17500000000000002 No Hit GGGAGAGGAGGAGCACGGCGCCGTAGAGCGGCGCGCCCATGGCGGCGGCG 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGAGATCGCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 4 (97% over 38bp) GTACGGCCTCGCCGTCGTCGGCTGCCTCGGGTCGTTCGCCCCCGTGCTGG 7 0.17500000000000002 No Hit TTTACTTTCTCTTCTCCTTCCTTAGTACCTGATTGACCATCACCGGCAGC 7 0.17500000000000002 No Hit GTCCTCCTCGCCGCTGATGAACTTGCCGGTGCCGGGGTTGATGGCGGCGA 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGAGATCTGGTAT 6 0.15 TruSeq Adapter, Index 4 (97% over 38bp) CATGCATCTGCAAAATTCAAAAGGAGAAGAAAAGAAGGCCCAATCCAAAA 6 0.15 No Hit GGCATGTTGCAGCCGTTGCAGCCGCTGCCGCACTTGCAGGATGACCCGCA 6 0.15 No Hit GTTTCCTTGTAGTCGCAGACGCGCTTCTCTTGATCTTCTTTTTCGAGGTT 6 0.15 No Hit CGGGCTTCCCAGCATCCACTAGTTCCCTTTTCTCTTTTCCTTTGTCCTCT 5 0.125 No Hit GGCCAGATGCGGCAGCGGCCAAGCCGAGGGCGGCCGGATCCGGCGGTTAG 5 0.125 No Hit ACTCTGAGCACTATACACAGACGTTCCTGCGATACCTCTCTCTAGTCTTT 5 0.125 No Hit GGTTAACATAATCAAGCACACAGCCACAACAGTAAATAGATAAGCAAAGC 5 0.125 No Hit GGAACAAGAACGTCGAGAGCAGCGCGTTCTTGGACTTCCCAAGCAAGACA 5 0.125 No Hit GCGCGTTGTTGCGGGAGAAGAAGATCTCCACTGAGCCTGTAGCCCACCGG 5 0.125 No Hit CGATCACGTCCAGCGGCGAGCTGTCGCCGCGCCCGTGCCAGCAGCGGAGG 5 0.125 No Hit TAGGAAACGACTTCTTGGCCTGCTCGAGCGACCGCGCGTGGCCGAAGTGG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGAGATCGGGTAT 5 0.125 TruSeq Adapter, Index 4 (97% over 38bp) GTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCAT 5 0.125 No Hit TGTACAAACAAAAGTGAAGGAAAAAAAGAATACAAGTCTTGTAAGCAACA 5 0.125 No Hit GCTTTGACATGTTCAGTCAAGGCATTTACAAGAGATTCCCTTGTCTTAAT 5 0.125 No Hit GGGGCACTTGATGGCAATGACAGCCATTGTTGGGCCGCCGACATAGCCAG 5 0.125 No Hit GCCTGATCTCGTCGTCGTTGTCGAGGCAGGGAATGTACTCCCTCATGGTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.175 0.0 0.0 0.0 0.0 50-51 0.25 0.0 0.0 0.0 0.0 52-53 0.25 0.0 0.0 0.0 0.0 54-55 0.275 0.0 0.0 0.0 0.0 56-57 0.3125 0.0 0.0 0.0 0.0 58-59 0.35 0.0 0.0 0.0 0.0 60-61 0.4375 0.0 0.0 0.0 0.0 62-63 0.475 0.0 0.0 0.0 0.0 64-65 0.475 0.0 0.0 0.0 0.0 66-67 0.575 0.0 0.0 0.0 0.0 68-69 0.6 0.0 0.0 0.0 0.0 70-71 0.6625000000000001 0.0 0.0 0.0 0.0 72-73 0.7625 0.0 0.0 0.0 0.0 74-75 1.0 0.0 0.0 0.0 0.0 76-77 1.275 0.0 0.0 0.0 0.0 78-79 1.5125 0.0 0.0 0.0 0.0 80-81 1.7125 0.0 0.0 0.0 0.0 82-83 1.975 0.0 0.0 0.0 0.0 84-85 2.3125 0.0 0.0 0.0 0.0 86-87 2.8375000000000004 0.0 0.0 0.0 0.0 88-89 3.175 0.0 0.0 0.0 0.0 90-91 3.575 0.0 0.0 0.0 0.0 92-93 3.8125 0.0 0.0 0.0 0.0 94-95 4.387499999999999 0.0 0.0 0.0 0.0 96-97 5.0125 0.0 0.0 0.0 0.0 98-99 5.65 0.0 0.0 0.0 0.0 100-101 6.3625 0.0 0.0 0.0 0.0 102-103 6.9625 0.0 0.0 0.0 0.0 104-105 7.7125 0.0 0.0 0.0 0.0 106-107 8.35 0.0 0.0 0.0 0.0 108-109 9.0 0.0 0.0 0.0 0.0 110-111 9.600000000000001 0.0 0.0 0.0 0.0 112-113 10.4875 0.0 0.0 0.0 0.0 114-115 11.4375 0.0 0.0 0.0 0.0 116-117 12.2125 0.0 0.0 0.0 0.0 118-119 12.9625 0.0 0.0 0.0 0.0 120-121 13.899999999999999 0.0 0.0 0.0 0.0 122-123 14.9 0.0 0.0 0.0 0.0 124-125 15.837499999999999 0.0 0.0 0.0 0.0 126-127 16.5625 0.0 0.0 0.0 0.0 128-129 17.4 0.0 0.0 0.0 0.0 130-131 18.2 0.0 0.0 0.0 0.0 132-133 18.95 0.0 0.0 0.0 0.0 134-135 19.8625 0.0 0.0 0.0 0.0 136-137 20.7 0.0 0.0 0.0 0.0 138-139 21.3875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGA 10 0.006830828 145.0 4 GATCGAG 25 8.7132835E-4 87.0 5 >>END_MODULE SRR13165382 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165382_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.888 37.0 37.0 37.0 37.0 37.0 2 36.0145 37.0 37.0 37.0 37.0 37.0 3 36.027 37.0 37.0 37.0 37.0 37.0 4 35.9285 37.0 37.0 37.0 37.0 37.0 5 36.0415 37.0 37.0 37.0 37.0 37.0 6 36.027 37.0 37.0 37.0 37.0 37.0 7 35.9935 37.0 37.0 37.0 37.0 37.0 8 35.961 37.0 37.0 37.0 37.0 37.0 9 36.0045 37.0 37.0 37.0 37.0 37.0 10-14 35.822700000000005 37.0 37.0 37.0 37.0 37.0 15-19 35.7995 37.0 37.0 37.0 37.0 37.0 20-24 35.68545 37.0 37.0 37.0 37.0 37.0 25-29 35.48225 37.0 37.0 37.0 37.0 37.0 30-34 35.46665 37.0 37.0 37.0 37.0 37.0 35-39 35.32445 37.0 37.0 37.0 37.0 37.0 40-44 35.41785 37.0 37.0 37.0 37.0 37.0 45-49 35.3797 37.0 37.0 37.0 37.0 37.0 50-54 35.32165 37.0 37.0 37.0 37.0 37.0 55-59 35.35165 37.0 37.0 37.0 37.0 37.0 60-64 35.40405 37.0 37.0 37.0 37.0 37.0 65-69 35.3039 37.0 37.0 37.0 37.0 37.0 70-74 35.227549999999994 37.0 37.0 37.0 34.6 37.0 75-79 35.199850000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.257400000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.2555 37.0 37.0 37.0 37.0 37.0 90-94 35.30675 37.0 37.0 37.0 37.0 37.0 95-99 35.254200000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.23545 37.0 37.0 37.0 37.0 37.0 105-109 35.183350000000004 37.0 37.0 37.0 34.6 37.0 110-114 35.123949999999994 37.0 37.0 37.0 34.6 37.0 115-119 35.013549999999995 37.0 37.0 37.0 27.4 37.0 120-124 34.88875 37.0 37.0 37.0 25.0 37.0 125-129 34.8157 37.0 37.0 37.0 25.0 37.0 130-134 34.621449999999996 37.0 37.0 37.0 25.0 37.0 135-139 34.540299999999995 37.0 37.0 37.0 25.0 37.0 140-144 34.177550000000004 37.0 37.0 37.0 25.0 37.0 145-149 33.8514 37.0 37.0 37.0 25.0 37.0 150-151 33.567 37.0 37.0 37.0 18.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 16.0 14 18.0 15 12.0 16 10.0 17 10.0 18 5.0 19 11.0 20 12.0 21 7.0 22 12.0 23 12.0 24 9.0 25 14.0 26 17.0 27 21.0 28 25.0 29 24.0 30 31.0 31 45.0 32 85.0 33 140.0 34 235.0 35 603.0 36 2408.0 37 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 60.97744360902255 11.478696741854638 6.641604010025063 20.902255639097746 2 33.75 12.45 28.225 25.575 3 29.275000000000002 19.225 28.549999999999997 22.95 4 30.275000000000002 29.2 16.25 24.275 5 25.775 34.175 17.45 22.6 6 27.450000000000003 29.625 18.075 24.85 7 27.825 17.125 30.55 24.5 8 24.975 20.05 21.325 33.650000000000006 9 25.874999999999996 20.875 23.200000000000003 30.049999999999997 10-14 28.775000000000002 22.634999999999998 21.529999999999998 27.060000000000002 15-19 28.775000000000002 23.425 21.265 26.534999999999997 20-24 28.001400070003502 22.88114405720286 22.461123056152807 26.65633281664083 25-29 27.596899224806204 24.711177794448613 21.40035008752188 26.291572893223307 30-34 27.969195379306893 23.258488773315996 22.19832974946242 26.573986097914688 35-39 27.146357317865892 24.201210060503026 22.4011200560028 26.251312565628282 40-44 28.73431014652198 23.258488773315996 21.853277991698754 26.153923088463273 45-49 27.16771677167717 23.72237223722372 22.782278227822783 26.327632763276327 50-54 26.856342817140856 24.046202310115504 23.201160058002902 25.896294814740738 55-59 27.89697424356089 23.460865216304075 21.72043010752688 26.921730432608154 60-64 28.066403320166007 23.301165058252913 22.4011200560028 26.23131156557828 65-69 27.805000000000003 23.255 22.835 26.105 70-74 26.94673668417104 24.15603900975244 22.37559389847462 26.521630407601897 75-79 28.374256138420762 23.313497024553683 22.093313997099564 26.21893283992599 80-84 28.335 23.745 22.395 25.525 85-89 27.775555111022204 24.55991198239648 21.679335867173435 25.985197039407883 90-94 28.30641532076604 23.71618580929046 22.536126806340317 25.441272063603183 95-99 28.68 23.605 22.225 25.490000000000002 100-104 28.75718929732433 24.241060265066267 21.790447611902973 25.211302825706426 105-109 29.41235308827207 23.925981495373843 22.02050512628157 24.641160290072516 110-114 29.98649932496625 24.18620931046552 21.28606430321516 24.54122706135307 115-119 30.152538134533636 23.95598899724931 22.12553138284571 23.765941485371343 120-124 30.222555638909725 24.241060265066267 22.200550137534382 23.335833958489623 125-129 30.136027205441092 24.33986797359472 22.669533906781357 22.854570914182837 130-134 31.38098334417046 23.73330665733007 22.397839243735305 22.487870754764167 135-139 31.994598379513857 23.49704911473442 22.15664699409823 22.351705511653496 140-144 33.38166908345418 24.016200810040502 21.171058552927647 21.43107155357768 145-149 33.53506051815545 23.25697709312794 21.931579473842152 21.27638291487446 150-151 35.217608804402204 24.212106053026513 20.710355177588795 19.85992996498249 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.5 4 1.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 0.0 20 0.5 21 1.0 22 1.5 23 2.0 24 2.5 25 1.5 26 0.0 27 0.0 28 2.0 29 4.0 30 2.5 31 2.0 32 4.5 33 9.0 34 12.0 35 17.0 36 26.5 37 28.5 38 33.5 39 57.5 40 85.5 41 103.5 42 124.0 43 122.5 44 129.5 45 140.5 46 135.5 47 151.5 48 171.5 49 169.5 50 139.0 51 130.0 52 128.0 53 124.0 54 119.5 55 97.5 56 90.0 57 100.5 58 103.5 59 93.0 60 90.0 61 83.0 62 83.5 63 94.5 64 92.5 65 85.5 66 80.5 67 79.0 68 77.0 69 87.0 70 78.0 71 53.0 72 48.0 73 42.0 74 38.5 75 42.0 76 30.5 77 19.0 78 15.5 79 10.0 80 7.5 81 6.0 82 3.5 83 1.0 84 0.5 85 1.5 86 3.0 87 2.5 88 2.5 89 3.0 90 4.0 91 5.0 92 7.0 93 8.0 94 6.0 95 4.5 96 4.5 97 4.5 98 2.5 99 2.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.034999999999999996 135-139 0.03 140-144 0.005 145-149 0.03 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.02499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80041652204096 53.87499999999999 2 16.730301978479694 24.099999999999998 3 5.553627212773343 12.0 4 1.94376952447067 5.6000000000000005 5 0.4512322110378341 1.625 6 0.06942034015966678 0.3 7 0.3471017007983339 1.7500000000000002 8 0.03471017007983339 0.2 9 0.03471017007983339 0.22499999999999998 >10 0.03471017007983339 0.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 13 0.325 No Hit GGGGCCTGCTTCAAGTGCGGCGGCAACTGCTACAACTGCGGCGAGCCTGG 9 0.22499999999999998 No Hit TGAGTTTAGCATGGGCACTGGGAGCAACAGGCGAGATTCCATTGCTGGAT 8 0.2 No Hit GGGAAAGCAGAACCGCACGCCGACAATCGTCATTGTTTGGTCCATCCTCC 7 0.17500000000000002 No Hit GGAGAGAGAGAAGAGAGATGAAGGAGGCGACTACGCCGGGGGGCGCCAGC 7 0.17500000000000002 No Hit GCACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTT 7 0.17500000000000002 No Hit CACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTC 7 0.17500000000000002 No Hit GTTCTCGAAGGACTCGTCGGTGCCGGCGTCGGGCGCGGCGGCGATAGAGA 7 0.17500000000000002 No Hit GCGAGGGAGGGCCGCTGTTTGGGTTCACCAAGTCGAACGAGCTGTTCGTG 7 0.17500000000000002 No Hit GTTGCATCTAAACCAAGAGTAGAGGTCCAAGGGGGTGACTTGCGATCTTT 7 0.17500000000000002 No Hit GCTAGCACCACCAGATCCTTCCTCCACCACGGCAAACACAAGCAGCACCT 7 0.17500000000000002 No Hit CCAGACGCCAGTGGGTCCGAGAAGAAGATTTGGCTAAATCATTAAAGCTC 7 0.17500000000000002 No Hit ACACACAAGCCTAGAAGCCTTCAGCTTAATCTTCTTCCTAAGCAAGTTCG 7 0.17500000000000002 No Hit GGTAAGTGATAGTACATCAGTCACATCTACAGAAGATGTTTTAGAACCAA 6 0.15 No Hit GGAGACGACACAGAGTACGGTACGAGTGTACACAGAGAGGGAAGAGAGAG 6 0.15 No Hit CAGATCCACCACCTTGGCTCGCAAAGTCTTCCGGCTCCTCAAGTGGGTGA 5 0.125 No Hit GTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCT 5 0.125 No Hit CTGCATACAAGAAGTCGCCTCCGTTCCTTTTCTCCAGCAGCTCTTCTCAA 5 0.125 No Hit GGGACTTCCAGGGAGACCAAGAGGAAGAAGAAGCCATTTGTCTCAGGGTC 5 0.125 No Hit GTGGAGTCTCTCCTGAAGCAGATCGAGTACCTGATCCGCTCCAAGTGGGT 5 0.125 No Hit GCCCAAGGAGCCTGCACCGAAGCCTGAAGCGGAGGAGAGCGGCGGCGGTC 5 0.125 No Hit CAGAAGTGAAGCTGCCGTTGAGTTTTGTTGTCACGGTTTGCTCGAGATGT 5 0.125 No Hit CGGATCCTCGCTTGGACATGGCGTCGCCCATGCTCTCCACGGCCATGGCG 5 0.125 No Hit CTTGCGCTCTCCCCCGAACCTTCTCGAGCCGCTCCCATGGCCGACGCCAA 5 0.125 No Hit GATCAAGGAAGTTTAACATCAGAGACGACTACTGCAACCTGCAGCCTGTG 5 0.125 No Hit TGGGCTTGCAGGGAACCGACCTGCTCTGCGGCCCCCGCATCCTCCGCGCC 5 0.125 No Hit GTCAAGAATGGGCGTCCTCCTGGTGCTCTTACCGTCCCTCGGGACCTTCT 5 0.125 No Hit GTTGTTGTGTATGTGACAGTTCCAAATCGGGAAGCAGGCAAAAAGCTATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.075 0.0 0.0 0.0 0.0 40-41 0.075 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.1 0.0 0.0 0.0 0.0 46-47 0.1 0.0 0.0 0.0 0.0 48-49 0.175 0.0 0.0 0.0 0.0 50-51 0.25 0.0 0.0 0.0 0.0 52-53 0.25 0.0 0.0 0.0 0.0 54-55 0.275 0.0 0.0 0.0 0.0 56-57 0.3125 0.0 0.0 0.0 0.0 58-59 0.35 0.0 0.0 0.0 0.0 60-61 0.4375 0.0 0.0 0.0 0.0 62-63 0.475 0.0 0.0 0.0 0.0 64-65 0.475 0.0 0.0 0.0 0.0 66-67 0.6 0.0 0.0 0.0 0.0 68-69 0.625 0.0 0.0 0.0 0.0 70-71 0.7124999999999999 0.0 0.0 0.0 0.0 72-73 0.8125 0.0 0.0 0.0 0.0 74-75 1.0499999999999998 0.0 0.0 0.0 0.0 76-77 1.325 0.0 0.0 0.0 0.0 78-79 1.5625 0.0 0.0 0.0 0.0 80-81 1.7374999999999998 0.0 0.0 0.0 0.0 82-83 2.0 0.0 0.0 0.0 0.0 84-85 2.3375 0.0 0.0 0.0 0.0 86-87 2.8375000000000004 0.0 0.0 0.0 0.0 88-89 3.175 0.0 0.0 0.0 0.0 90-91 3.5999999999999996 0.0 0.0 0.0 0.0 92-93 3.8375000000000004 0.0 0.0 0.0 0.0 94-95 4.4125 0.0 0.0 0.0 0.0 96-97 5.0375 0.0 0.0 0.0 0.0 98-99 5.675 0.0 0.0 0.0 0.0 100-101 6.4125 0.0 0.0 0.0 0.0 102-103 7.012499999999999 0.0 0.0 0.0 0.0 104-105 7.7625 0.0 0.0 0.0 0.0 106-107 8.4 0.0 0.0 0.0 0.0 108-109 9.0625 0.0 0.0 0.0 0.0 110-111 9.675 0.0 0.0 0.0 0.0 112-113 10.587499999999999 0.0 0.0 0.0 0.0 114-115 11.6125 0.0 0.0 0.0 0.0 116-117 12.425 0.0 0.0 0.0 0.0 118-119 13.2 0.0 0.0 0.0 0.0 120-121 14.1375 0.0 0.0 0.0 0.0 122-123 15.15 0.0 0.0 0.0 0.0 124-125 16.1375 0.0 0.0 0.0 0.0 126-127 16.925 0.0 0.0 0.0 0.0 128-129 17.825 0.0 0.0 0.0 0.0 130-131 18.6375 0.0 0.0 0.0 0.0 132-133 19.4 0.0 0.0 0.0 0.0 134-135 20.3125 0.0 0.0 0.0 0.0 136-137 21.175 0.0 0.0 0.0 0.0 138-139 21.9125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253979 spots for SRR13165382.sra Written 1253979 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra Read 1253967 spots for SRR13165382.sra Written 1253967 spots for SRR13165382.sra SRR ids: ['SRR13165382.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rtdhpo_l SRR13165382.sra spots: 25079352 blocks: [[1, 1253967], [1253968, 2507934], [2507935, 3761901], [3761902, 5015868], [5015869, 6269835], [6269836, 7523802], [7523803, 8777769], [8777770, 10031736], [10031737, 11285703], [11285704, 12539670], [12539671, 13793637], [13793638, 15047604], [15047605, 16301571], [16301572, 17555538], [17555539, 18809505], [18809506, 20063472], [20063473, 21317439], [21317440, 22571406], [22571407, 23825373], [23825374, 25079352]] SRR13165382 file size 8501360 SRR13165382 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165382 SRR13165382_1.fastq SRR13165382_2.fastq Input file: SRR13165382_1.fastq Paired file: SRR13165382_2.fastq trimmed: SRR13165382-trimmed-pair1.fastq, SRR13165382-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 17:05:18 2024 >> started Sat Dec 7 17:05:48 2024 >> done (29.853s) 25079352 read pairs processed; of these: 2281 ( 0.01%) short read pairs filtered out after trimming by size control 332116 ( 1.32%) empty read pairs filtered out after trimming by size control 24744955 (98.67%) read pairs available; of these: 5937248 (23.99%) trimmed read pairs available after processing 18807707 (76.01%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 141 0.00% 19 108 0.00% 20 192 0.00% 21 192 0.00% 22 249 0.00% 23 314 0.00% 24 334 0.00% 25 303 0.00% 26 425 0.00% 27 421 0.00% 28 485 0.00% 29 464 0.00% 30 514 0.00% 31 430 0.00% 32 429 0.00% 33 523 0.00% 34 482 0.00% 35 502 0.00% 36 576 0.00% 37 613 0.00% 38 632 0.00% 39 841 0.00% 40 926 0.00% 41 964 0.00% 42 1072 0.00% 43 1067 0.00% 44 1065 0.00% 45 1249 0.01% 46 1241 0.01% 47 1403 0.01% 48 1778 0.01% 49 1968 0.01% 50 2395 0.01% 51 2603 0.01% 52 3057 0.01% 53 3141 0.01% 54 3588 0.01% 55 3738 0.02% 56 4022 0.02% 57 4513 0.02% 58 5257 0.02% 59 6033 0.02% 60 7049 0.03% 61 8186 0.03% 62 9307 0.04% 63 10076 0.04% 64 10528 0.04% 65 11023 0.04% 66 12231 0.05% 67 12962 0.05% 68 14868 0.06% 69 16676 0.07% 70 18680 0.08% 71 20270 0.08% 72 23456 0.09% 73 24973 0.10% 74 25932 0.10% 75 27102 0.11% 76 27972 0.11% 77 29781 0.12% 78 32413 0.13% 79 34914 0.14% 80 37300 0.15% 81 40697 0.16% 82 42792 0.17% 83 45820 0.19% 84 46778 0.19% 85 47495 0.19% 86 46823 0.19% 87 47947 0.19% 88 49879 0.20% 89 51489 0.21% 90 55242 0.22% 91 58487 0.24% 92 60972 0.25% 93 63519 0.26% 94 64749 0.26% 95 64867 0.26% 96 64745 0.26% 97 64217 0.26% 98 63820 0.26% 99 67147 0.27% 100 68011 0.27% 101 71112 0.29% 102 74942 0.30% 103 75345 0.30% 104 76741 0.31% 105 77256 0.31% 106 74988 0.30% 107 75380 0.30% 108 76041 0.31% 109 76552 0.31% 110 76641 0.31% 111 80674 0.33% 112 83268 0.34% 113 84163 0.34% 114 86970 0.35% 115 84857 0.34% 116 86137 0.35% 117 85530 0.35% 118 84084 0.34% 119 83649 0.34% 120 86126 0.35% 121 87199 0.35% 122 88122 0.36% 123 91652 0.37% 124 92271 0.37% 125 90705 0.37% 126 91858 0.37% 127 88970 0.36% 128 87890 0.36% 129 88752 0.36% 130 88429 0.36% 131 88100 0.36% 132 91174 0.37% 133 91853 0.37% 134 92720 0.37% 135 95497 0.39% 136 94301 0.38% 137 91526 0.37% 138 91677 0.37% 139 90593 0.37% 140 88521 0.36% 141 90395 0.37% 142 93419 0.38% 143 92761 0.37% 144 95072 0.38% 145 98721 0.40% 146 97156 0.39% 147 94612 0.38% 148 90470 0.37% 149 90084 0.36% 150 90847 0.37% 151 18807707 76.01% 24744955 reads passed initial QC criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=2.77 fanout-score-rank=25 prefix-density=0.61 prefix-fanout=2.6 sequence=TGCCGCACTTGCAGGATGACCCGCAGTTGCAGTTTCCTCCGCAGCAAGACATCTTCGGTCGAGTGCTCGAACTTGCTTAGGAAGAAGATTAAGCTGAAGGCTTCTAGGCTTGTGTGTGCTTC criterion=fanout-score sequence-density=0.03 sequence-density-rank=26 fanout-score=511.39 fanout-score-rank=1 prefix-density=0.80 prefix-fanout=20.0 sequence=GGCGGCGGCGAACCGCCCCCGTCGCCGCGATCCGAACACTTCACCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGCTATATACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTCGCCTAAACGGCGATAGTCCCTCTAAGAAGCTAGCTGCGGAGGGATGGCTCCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGACCTGGTAAG criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=7.05 fanout-score-rank=16 prefix-density=0.34 prefix-fanout=4.8 sequence=GGCAAGACCATCACCCTTGAGGTGGAGTCATCTGACACCATCGACAA criterion=fanout-score sequence-density=0.11 sequence-density-rank=19 fanout-score=196.61 fanout-score-rank=1 prefix-density=0.93 prefix-fanout=24.2 sequence=CGCCGCCGCCGTC SRR13165382 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:06:33 Started mapping on | Dec 07 17:06:34 Finished on | Dec 07 17:08:59 Mapping speed, Million of reads per hour | 614.36 Number of input reads | 24744955 Average input read length | 284 UNIQUE READS: Uniquely mapped reads number | 22793761 Uniquely mapped reads % | 92.11% Average mapped length | 282.95 Number of splices: Total | 20207413 Number of splices: Annotated (sjdb) | 18813910 Number of splices: GT/AG | 19941921 Number of splices: GC/AG | 217864 Number of splices: AT/AC | 9596 Number of splices: Non-canonical | 38032 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.85 Insertion rate per base | 0.01% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 292493 % of reads mapped to multiple loci | 1.18% Number of reads mapped to too many loci | 62135 % of reads mapped to too many loci | 0.25% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.41% % of reads unmapped: other | 1.04% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1658984 1658984 1658984 N_multimapping 292493 292493 292493 N_noFeature 743423 22129990 1028088 N_ambiguous 449242 3625 70296 UnstrandedReadsAssigned:21601096 PositiveStrandReadsAssigned:660146 NegativeStrandReadsAssigned:21695377 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=140 echo kmer=135 SRR13165382 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165382-trimmed-pair1.fastq SRR13165382-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 24,744,955 reads, 22,249,881 reads pseudoaligned [quant] estimated average fragment length: 234.794 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,213 rounds 52973 SRR13165382.ke.tsv 35125 SRR13165382.se.tsv 88098 total ==> SRR13165382.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 702.909 0 0 PNS24247 1044 810.206 65.8985 5.31924 PNS24249 1928 1694.21 251.247 9.69851 PNS24246 1044 810.206 65.8985 5.31924 PNS24248 1044 810.206 65.8985 5.31924 PNS24244 1471 1237.21 73.0572 3.86181 PNS24243 293 120.743 0 0 KQK14069 1603 1369.21 361.298 17.2571 KQK14071 474 262.307 2.47291 0.61655 ==> SRR13165382.se.tsv <== BRADI_1g14170v3 393 BRADI_1g53295v3 195 BRADI_1g59795v3 349 BRADI_1g07683v3 0 BRADI_1g00485v3 0 BRADI_1g20270v3 560 BRADI_1g74790v3 610 BRADI_1g09890v3 0 BRADI_1g77505v3 307 BRADI_1g48960v3 0 SRR13165382 completed mapping pipeline successfully