Starting /dee2/code/volunteer_pipeline.sh SRR13165383 current disk space = 1541468934144 free memory = 1599169892 SRR13165383 SRAfilesize e07bb52d1a2fb8f8c46ac78aaa19bad3 SRR13165383.sra SRR13165383.sra file validated SRR13165383 is paired end SRR13165383 is conventional basespace SRR13165383 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165383_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5635 37.0 37.0 37.0 37.0 37.0 2 35.92675 37.0 37.0 37.0 37.0 37.0 3 36.517 37.0 37.0 37.0 37.0 37.0 4 36.49 37.0 37.0 37.0 37.0 37.0 5 36.446 37.0 37.0 37.0 37.0 37.0 6 36.557 37.0 37.0 37.0 37.0 37.0 7 36.4655 37.0 37.0 37.0 37.0 37.0 8 36.5495 37.0 37.0 37.0 37.0 37.0 9 36.517 37.0 37.0 37.0 37.0 37.0 10-14 36.505 37.0 37.0 37.0 37.0 37.0 15-19 36.4609 37.0 37.0 37.0 37.0 37.0 20-24 36.4803 37.0 37.0 37.0 37.0 37.0 25-29 36.43300000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.365899999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.361599999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.3071 37.0 37.0 37.0 37.0 37.0 45-49 36.176700000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.2639 37.0 37.0 37.0 37.0 37.0 55-59 36.126799999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.094 37.0 37.0 37.0 37.0 37.0 65-69 35.9311 37.0 37.0 37.0 37.0 37.0 70-74 36.02909999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.194900000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.110200000000006 37.0 37.0 37.0 37.0 37.0 85-89 36.1913 37.0 37.0 37.0 37.0 37.0 90-94 36.1491 37.0 37.0 37.0 37.0 37.0 95-99 36.113099999999996 37.0 37.0 37.0 37.0 37.0 100-104 36.117200000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.04559999999999 37.0 37.0 37.0 37.0 37.0 110-114 36.0675 37.0 37.0 37.0 37.0 37.0 115-119 36.0991 37.0 37.0 37.0 37.0 37.0 120-124 35.98350000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.9609 37.0 37.0 37.0 37.0 37.0 130-134 35.8872 37.0 37.0 37.0 37.0 37.0 135-139 35.8164 37.0 37.0 37.0 37.0 37.0 140-144 35.7575 37.0 37.0 37.0 37.0 37.0 145-149 35.4884 37.0 37.0 37.0 37.0 37.0 150-151 35.305 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 2.0 23 2.0 24 3.0 25 6.0 26 11.0 27 9.0 28 13.0 29 28.0 30 27.0 31 43.0 32 44.0 33 95.0 34 175.0 35 351.0 36 2765.0 37 424.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.324999999999996 10.975 6.925000000000001 30.775000000000002 2 25.233881163084703 11.47914032869785 30.164348925410874 33.122629582806574 3 20.25 14.7 28.425 36.625 4 24.175 20.724999999999998 23.724999999999998 31.374999999999996 5 29.75 26.474999999999998 22.025 21.75 6 27.800000000000004 30.575000000000003 19.75 21.875 7 18.775 25.874999999999996 37.15 18.2 8 19.775000000000002 26.525 27.800000000000004 25.900000000000002 9 21.65 22.225 32.275 23.849999999999998 10-14 24.015 27.334999999999997 24.265 24.385 15-19 23.865 25.595000000000002 24.64 25.900000000000002 20-24 23.635 25.135 25.635 25.595000000000002 25-29 23.294999999999998 25.635 24.725 26.345000000000002 30-34 23.65 24.97 24.884999999999998 26.495 35-39 23.235 25.014999999999997 24.895 26.855 40-44 22.46 25.885 25.019999999999996 26.634999999999998 45-49 23.34 24.759999999999998 25.09 26.810000000000002 50-54 24.060000000000002 24.77 24.51 26.66 55-59 23.565 24.725 25.455 26.255 60-64 24.09 24.45 25.705 25.755 65-69 24.505 26.119999999999997 23.61 25.765 70-74 25.385 24.13 25.275 25.21 75-79 25.39 24.505 24.235 25.869999999999997 80-84 25.89 24.285 24.465 25.36 85-89 25.21 26.245 24.04 24.505 90-94 26.365 25.014999999999997 23.665 24.955 95-99 25.695 24.54 23.39 26.375 100-104 26.075 24.11 23.86 25.955000000000002 105-109 26.525 24.605 23.255 25.615 110-114 25.55 24.98 24.3 25.169999999999998 115-119 25.715 24.84 23.830000000000002 25.615 120-124 25.814999999999998 24.87 23.580000000000002 25.735000000000003 125-129 25.405 23.985 23.435 27.175 130-134 25.509999999999998 25.074999999999996 23.615 25.8 135-139 25.319999999999997 24.09 24.395 26.195 140-144 26.405 25.2 22.605 25.790000000000003 145-149 25.705 24.65 23.365 26.279999999999998 150-151 25.724999999999998 24.875 23.275000000000002 26.125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 0.5 24 0.5 25 1.5 26 2.0 27 1.0 28 4.0 29 4.0 30 3.5 31 6.5 32 9.0 33 16.0 34 23.0 35 22.5 36 31.5 37 44.0 38 70.0 39 98.0 40 106.0 41 112.0 42 153.0 43 177.0 44 178.5 45 191.0 46 192.0 47 199.5 48 198.5 49 201.0 50 197.5 51 171.0 52 130.5 53 115.5 54 125.0 55 105.5 56 88.5 57 81.5 58 73.5 59 67.5 60 57.0 61 56.5 62 63.0 63 58.5 64 61.5 65 71.5 66 75.5 67 70.5 68 54.5 69 39.5 70 40.5 71 41.0 72 25.5 73 23.0 74 17.5 75 11.0 76 8.0 77 4.5 78 4.0 79 5.5 80 3.5 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.875 #Duplication Level Percentage of deduplicated Percentage of total 1 69.7943925233645 46.675 2 20.037383177570092 26.8 3 6.429906542056074 12.9 4 2.130841121495327 5.7 5 0.7102803738317757 2.375 6 0.4485981308411215 1.7999999999999998 7 0.22429906542056074 1.05 8 0.07476635514018691 0.4 9 0.0 0.0 >10 0.14953271028037382 2.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACCTATATCTCGTAT 44 1.0999999999999999 TruSeq Adapter, Index 4 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACCTATATCGCGTAT 28 0.7000000000000001 TruSeq Adapter, Index 4 (97% over 38bp) GCCTGGTCAACGGCGTCCTCAATCTTCTTCTTGTCGTCCGCTGGCAGCTT 10 0.25 No Hit CCAGCTTCAATTTTCAGCATCAAATCATCTTGTGACAGCTCATAGTTATC 10 0.25 No Hit CCCCTCTGTAGTATGATCGAGTTATAGATCTGAATGATTCTTGGCCAGCC 8 0.2 No Hit GGTAGTAATCCGATCGATGCCTTGCCAGTCTGAAATCACAAAGCCCCTAA 8 0.2 No Hit GTTTCCTTATTATCCCTGATCTGCTGCTGGTTGTTGTCGAACTGGTCGGC 7 0.17500000000000002 No Hit GGTGCCGCCAAGCTTCAGTCTCAATGAGCCTTCCTTTTGTTTGCCAGGTG 7 0.17500000000000002 No Hit CTCCGACAGCGTGGCCGTGTCGTTGACGCTCTTGCACCTGGACTTGAGCT 7 0.17500000000000002 No Hit ATTTGGAAGCAATAGAACACGTGAACCATAAGCCACAATTAGCTTGCTAA 7 0.17500000000000002 No Hit CAATAGACTTGAAAAGTTGGGGATGGCGCAGTGGGAGTTCGACCAGCTCT 7 0.17500000000000002 No Hit GTCAAGTTATCACGGAGAAGTTGCATGATCAAGGTGCTGTCTTTGTAAGA 7 0.17500000000000002 No Hit CCTTCAAGCAATGGCAATGACAACTTTGCGATCGATTGCACAAAAATGCC 6 0.15 No Hit GGTAAACAGAATGAAGTGCTTGAAGCAGGTGTTAAGATGAGCCTCCTCCT 6 0.15 No Hit GGACGGAATTATGCACGTACGGCCGGCCGGCGCGGCGCGCGAGAGAACCC 6 0.15 No Hit CCAGCAGTGTCCCAGCAGTAGAAACGGATCTTTCCACAGTTGGTGGTGAA 6 0.15 No Hit CCTCAGGCTCGTCCTTCTTCTCCTCCTCAGGCTCGTCCTTCTTCTCCTCC 6 0.15 No Hit GTCTTTTTCAGTGGTGTAGCCAGCCATGACATTATCTCTGCCTCGAAGCT 6 0.15 No Hit TTAGAACAAATCTTACAATTGTTTTCTTCTTTCAAATTATGACTATTGAT 6 0.15 No Hit TACAGGCGTCTGTCACTGAAAATCCCCACGAACTACTTACTGTTTGAACC 6 0.15 No Hit GCCGAGTTCTCCTTCTGCTCCTGTTACGCAGGGGCAAGGGCATGGGCTGC 6 0.15 No Hit CCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAGGC 6 0.15 No Hit TGGCAAACGTGAGGAATGAGTGTGCCTGCCTTACACCCCATGTCAGGTGT 6 0.15 No Hit CGGGAAAACTGGATATCTTCTTCTCATGTATCAAGATTAAGGGATCCTCT 6 0.15 No Hit GTCCATTATTACGAGTGATAGATCATTAAGCGGAATACAAGCAAAAGGTA 5 0.125 No Hit GGGAGAGAAAATGCAGATTGCTGCTAAACACCACCAGGCCAACACAAAGT 5 0.125 No Hit GGCACATACAGACTGACCGACCGACCGACCGACGGAAGGATTCAATTCAA 5 0.125 No Hit TGCTTATTTAAAATGGCGGCAATAAAGTTCATATCCTGAATGTCGAATGT 5 0.125 No Hit AAGGAAGCAGTGCTGGTAAGATATGTCTAAGTCTAACAACTAAAATTCTA 5 0.125 No Hit GAGTCCTTCAACGGGAAACCACATAGCCCTGGATTGTTGCTGTATATTGA 5 0.125 No Hit GCATTGAACTCTGTAAGTTTCTTTAGGATTCCTTCGAACTGAGCCGTCTC 5 0.125 No Hit GCTCATAGCTAGCAAAACATGGAAGGAGCCCGGCACACGCATGCATGCAT 5 0.125 No Hit CGGTGAATGGGCATTTCTTGTCAATGTAGGTACCTTCGATCGCTTCCCTT 5 0.125 No Hit GGGCCAACTATTCCCAGTAGTTTCATCTCCAGTTAGTGGCAAAGCAGCGT 5 0.125 No Hit GGCCTAGTATATTTCCGGCAGCACCACTCTCAGGTGTTTCATTCACCAAG 5 0.125 No Hit CGTCACCAGGACCATCATCAGCGATAAGCTTCTCATGAACCCTAAAGAGA 5 0.125 No Hit GTTTGGTTCACTAGGCATTCTCTGGATACGCAATCCAATCAACCCTAGTT 5 0.125 No Hit CTATCGATGGCCCTCCGCCCAGTAATCAACTCAAGTAAGACAACTCCAAA 5 0.125 No Hit GTTCACTCTTGCACAGAGCTGGTCTCAAAGAATGCACAACAACACAACTG 5 0.125 No Hit GCATAATAAATTGACCTTGTATTCCATAGTCCAAACTACCAAGGGTAAGC 5 0.125 No Hit CCTTCTTAGACCATGAATGATGAGGCTGGTAGGCGCCCATTGCAATTTCA 5 0.125 No Hit GTTATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGC 5 0.125 No Hit GCCGGATGTGGCGTGGGACGATACGGGTCTTCTTGTTGTCCCTCGCGGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.0625 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.15 0.0 0.0 0.0 0.0 60-61 0.16249999999999998 0.0 0.0 0.0 0.0 62-63 0.21250000000000002 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.25 0.0 0.0 0.0 0.0 68-69 0.275 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.375 0.0 0.0 0.0 0.0 74-75 0.475 0.0 0.0 0.0 0.0 76-77 0.6875 0.0 0.0 0.0 0.0 78-79 0.7875 0.0 0.0 0.0 0.0 80-81 0.9624999999999999 0.0 0.0 0.0 0.0 82-83 1.4 0.0 0.0 0.0 0.0 84-85 1.7625000000000002 0.0 0.0 0.0 0.0 86-87 2.1625 0.0 0.0 0.0 0.0 88-89 2.4625 0.0 0.0 0.0 0.0 90-91 2.925 0.0 0.0 0.0 0.0 92-93 3.325 0.0 0.0 0.0 0.0 94-95 3.4875 0.0 0.0 0.0 0.0 96-97 3.8125 0.0 0.0 0.0 0.0 98-99 4.225 0.0 0.0 0.0 0.0 100-101 4.9125 0.0 0.0 0.0 0.0 102-103 5.275 0.0 0.0 0.0 0.0 104-105 5.725 0.0 0.0 0.0 0.0 106-107 6.4 0.0 0.0 0.0 0.0 108-109 6.925000000000001 0.0 0.0 0.0 0.0 110-111 7.7125 0.0 0.0 0.0 0.0 112-113 8.375 0.0 0.0 0.0 0.0 114-115 8.9875 0.0 0.0 0.0 0.0 116-117 9.6375 0.0 0.0 0.0 0.0 118-119 10.225 0.0 0.0 0.0 0.0 120-121 10.837499999999999 0.0 0.0 0.0 0.0 122-123 11.4125 0.0 0.0 0.0 0.0 124-125 12.0625 0.0 0.0 0.0 0.0 126-127 12.662500000000001 0.0 0.0 0.0 0.0 128-129 13.4625 0.0 0.0 0.0 0.0 130-131 14.4375 0.0 0.0 0.0 0.0 132-133 15.5 0.0 0.0 0.0 0.0 134-135 16.65 0.0 0.0 0.0 0.0 136-137 17.55 0.0 0.0 0.0 0.0 138-139 18.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTAAT 10 0.006830828 145.0 3 TAATCCG 10 0.006830828 145.0 6 TCCGATC 10 0.006830828 145.0 9 ATCCGAT 10 0.006830828 145.0 8 GTAGTAA 10 0.006830828 145.0 2 >>END_MODULE SRR13165383 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13165383_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.875 37.0 37.0 37.0 37.0 37.0 2 36.1765 37.0 37.0 37.0 37.0 37.0 3 36.0205 37.0 37.0 37.0 37.0 37.0 4 35.972 37.0 37.0 37.0 37.0 37.0 5 36.112 37.0 37.0 37.0 37.0 37.0 6 36.1805 37.0 37.0 37.0 37.0 37.0 7 36.011 37.0 37.0 37.0 37.0 37.0 8 35.969 37.0 37.0 37.0 37.0 37.0 9 35.889 37.0 37.0 37.0 37.0 37.0 10-14 35.8946 37.0 37.0 37.0 37.0 37.0 15-19 35.8082 37.0 37.0 37.0 37.0 37.0 20-24 35.763149999999996 37.0 37.0 37.0 37.0 37.0 25-29 35.56295 37.0 37.0 37.0 37.0 37.0 30-34 35.44015 37.0 37.0 37.0 37.0 37.0 35-39 35.46565 37.0 37.0 37.0 37.0 37.0 40-44 35.43795 37.0 37.0 37.0 37.0 37.0 45-49 35.3643 37.0 37.0 37.0 37.0 37.0 50-54 35.380849999999995 37.0 37.0 37.0 37.0 37.0 55-59 35.43765 37.0 37.0 37.0 37.0 37.0 60-64 35.39045 37.0 37.0 37.0 37.0 37.0 65-69 35.35889999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.26445 37.0 37.0 37.0 37.0 37.0 75-79 35.23175 37.0 37.0 37.0 37.0 37.0 80-84 35.28189999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.44879999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.46155 37.0 37.0 37.0 37.0 37.0 95-99 35.520799999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.490449999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.43035 37.0 37.0 37.0 37.0 37.0 110-114 35.409549999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.37495 37.0 37.0 37.0 37.0 37.0 120-124 35.144949999999994 37.0 37.0 37.0 34.6 37.0 125-129 35.0866 37.0 37.0 37.0 27.4 37.0 130-134 34.780950000000004 37.0 37.0 37.0 25.0 37.0 135-139 34.763999999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.53635 37.0 37.0 37.0 25.0 37.0 145-149 34.2508 37.0 37.0 37.0 25.0 37.0 150-151 33.772 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 3.0 13 6.0 14 11.0 15 10.0 16 6.0 17 8.0 18 5.0 19 5.0 20 10.0 21 9.0 22 12.0 23 11.0 24 17.0 25 28.0 26 29.0 27 23.0 28 24.0 29 16.0 30 32.0 31 50.0 32 69.0 33 131.0 34 233.0 35 453.0 36 2526.0 37 271.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.14934409687185 20.76185671039354 9.611503531786076 22.47729566094854 2 34.825 19.825 22.0 23.35 3 26.025 23.0 30.049999999999997 20.925 4 29.5 29.425 19.175 21.9 5 30.475 30.599999999999998 18.95 19.975 6 26.5 34.425 18.6 20.474999999999998 7 27.675 18.95 30.95 22.425 8 27.35 21.425 22.325 28.9 9 26.125 21.475 26.05 26.35 10-14 28.065 24.795 21.915000000000003 25.224999999999998 15-19 28.29 24.385 23.525 23.799999999999997 20-24 28.060433238281057 23.95317424583521 23.622992645955275 24.363399869928458 25-29 27.795846885163872 24.34325744308231 23.952964723542657 23.90793094821116 30-34 27.547906138990342 25.171361384900187 23.69540201130735 23.58533046480212 35-39 27.530141577867827 24.98374105758167 23.152734003702037 24.33338336084847 40-44 27.447841096712867 24.155701205783757 23.565317456346627 24.831140241156753 45-49 26.665999599759854 24.15449269561737 24.47968781268761 24.699819891935164 50-54 27.615188353594476 24.478463154735106 23.11771474310871 24.788633748561708 55-59 28.996747560670507 24.323242431823868 23.047285464098074 23.632724543407555 60-64 27.98539196558107 24.35839711841513 23.627995397468606 24.028215518535195 65-69 27.81112444977991 24.129651860744296 23.559423769507802 24.499799919967987 70-74 27.91593695271454 25.328996747560673 23.18739054290718 23.567675756817614 75-79 27.40781507980187 25.056286586281086 23.590333716915996 23.94556461700105 80-84 28.47423711855928 24.137068534267133 23.856928464232116 23.53176588294147 85-89 28.710097067947565 24.28700090063044 23.671570099069346 23.331331932352647 90-94 27.945369953474408 25.233878633248285 23.44289359147531 23.377857821801992 95-99 28.159079539769884 24.772386193096548 23.461730865432717 23.60680340170085 100-104 28.84663497623217 25.564173129847383 22.98223667750813 22.60695521641231 105-109 28.501376032024016 24.95871903927946 23.087315486614962 23.45258944208156 110-114 28.660763419880936 24.923708039421683 23.347841312721997 23.067687227975387 115-119 29.306980235176383 24.90367775831874 22.952214160620464 22.837127845884414 120-124 28.98173630222667 25.063797848386287 23.43757818363773 22.516887665749312 125-129 29.260482337636347 25.412788952266585 23.401380966676673 21.925347743420396 130-134 31.546814792573684 25.66681679427513 22.949507081018865 19.83686133213231 135-139 31.600280224179343 25.07005604483587 22.422938350680543 20.906725380304245 140-144 32.04262344289359 24.593526439541748 22.587423082695484 20.776427034869176 145-149 32.55104083266613 24.799839871897518 22.052642113690954 20.596477181745396 150-151 33.77127127127127 23.936436436436438 22.42242242242242 19.86986986986987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 1.0 9 0.5 10 0.5 11 2.0 12 2.5 13 2.0 14 2.0 15 1.0 16 0.0 17 0.5 18 1.5 19 1.5 20 1.0 21 0.5 22 1.0 23 1.5 24 1.0 25 2.0 26 2.5 27 3.0 28 3.0 29 2.0 30 3.0 31 4.5 32 5.0 33 9.0 34 15.0 35 17.0 36 27.0 37 40.5 38 56.0 39 79.5 40 94.5 41 94.5 42 125.5 43 179.0 44 188.5 45 174.0 46 191.0 47 215.0 48 208.0 49 187.5 50 169.5 51 141.5 52 118.0 53 130.5 54 125.5 55 99.5 56 88.0 57 75.0 58 73.0 59 62.5 60 59.0 61 71.5 62 72.0 63 62.5 64 57.5 65 58.5 66 64.0 67 69.5 68 67.5 69 62.0 70 46.0 71 32.0 72 33.5 73 35.5 74 21.5 75 13.0 76 15.5 77 13.5 78 6.0 79 4.0 80 5.0 81 4.0 82 2.0 83 0.5 84 0.0 85 1.0 86 1.0 87 2.5 88 4.0 89 5.0 90 6.5 91 6.5 92 7.0 93 6.5 94 6.5 95 7.5 96 7.0 97 5.5 98 5.0 99 5.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8999999999999999 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.075 30-34 0.065 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.04 70-74 0.075 75-79 0.065 80-84 0.05 85-89 0.06999999999999999 90-94 0.055 95-99 0.05 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.08499999999999999 135-139 0.08 140-144 0.055 145-149 0.08 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.6 #Duplication Level Percentage of deduplicated Percentage of total 1 71.91091954022988 50.05 2 18.929597701149426 26.35 3 5.998563218390805 12.525 4 1.7600574712643677 4.9 5 0.610632183908046 2.125 6 0.43103448275862066 1.7999999999999998 7 0.17959770114942528 0.8750000000000001 8 0.07183908045977011 0.4 9 0.07183908045977011 0.44999999999999996 >10 0.035919540229885055 0.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 21 0.525 No Hit AGAAGACGTGCAATTTTCTTGTGGAATCTGCTGAATCTGGCAATTTGCAG 9 0.22499999999999998 No Hit GGAAGGCTGAGCAAGGATGACATTGAGAAGATGGTTCAGGATGCTGAGAA 9 0.22499999999999998 No Hit AGCAAATTCCCCTTCCTTCTTCCCTCCGGCTCTGCCCTATTGCTATTGGA 8 0.2 No Hit GTTATGGTCTCTTACTCTAGTTGGAATGGACAGAAAATGCACGCCAACCA 8 0.2 No Hit GCTTGCGCTTAACTTCTCAGTGTTCTACTATGAAATTCTGAACTCGCCCG 7 0.17500000000000002 No Hit TACCGTCCTTTGAGAAAAGGAGACCTTTTCCTTGTGAGGGGTGGAATGAG 7 0.17500000000000002 No Hit GGCTCCAACGCGCCAACGGGCTGCAAATCGCCGTGCTTTATATGTCCCTC 7 0.17500000000000002 No Hit AGCCGCAGGACCTACAGCCTCCTATTTGACTGGTTGTATCCATCTCGCAT 7 0.17500000000000002 No Hit CGCCGTGCTCTCCGGCGCGCACACGCTCGGCACGGCGCACTGCCCGTCCT 7 0.17500000000000002 No Hit GAGAGTAATGGATCTGAAAGTGCTAGGGATACTGAGGTTCCTCTACAAGC 6 0.15 No Hit GTTTTGTTGCTCCTCCTCGCCGCCCCCCCTCCGCACCGCCGTCGCCGCCT 6 0.15 No Hit GGCACTTATTCCCGTTCCGGTACACGCCGTCGCCGCTGCCGAGGTGGGAG 6 0.15 No Hit GTTGCGTCCACTGTGATTTTCCTCAGTGCCACTACAACTGCAGCCTTGCA 6 0.15 No Hit AATTTGTCAAGACAATTGATCCACAGCTAACCAATTACGATGAAGAAGGC 6 0.15 No Hit GCCATCTTCCGCCGCTACAAGTGGTAAATTCCAGATCCAATCCATGGATC 6 0.15 No Hit CTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTT 6 0.15 No Hit AGGCCGTCGTGCTCTTCCGCGCCGTCGCGCTCCGGCACATGCTCATCATG 6 0.15 No Hit ATGTTTACTAGGGAGGAACTTGAATTCATTGCTACTCTCTGCAAGGAAAA 6 0.15 No Hit TAACCTGGGTGAAGTTTATGCTTTTGGATTTATAAATGGTTTGAGAAGGT 6 0.15 No Hit GGGGGCACAGCTCCGAAAGCACATAGATGCAACCCTTGGCAGTGGAAACC 6 0.15 No Hit GTTTATCAATGCATACAAGAATGTGCTTGCTATTGCTGTGGAGACTGATT 6 0.15 No Hit GTTGGCTCGGTATCTTGTTAGCATTATCAGTTTTTAGAATTTTAGTTGTT 5 0.125 No Hit CAGAGTTACCAGTAGATATACAGCTATGTTTGCGTATACATATATGCTTG 5 0.125 No Hit CTTCTTAAGCCTTCGGCTCCCCGCTTCTGTATCTCCCACCCGCGCCGCCG 5 0.125 No Hit AATGCGCTGAAGACTCTGCCTGTCCTGTTGGATGCATCAGATATGCTGGC 5 0.125 No Hit AAGTGCAGCGGCCCTGGCTAGAAAATATCGGCGGTTCATGATCTATGTTC 5 0.125 No Hit CAAGAAAGTAGCAGAGGCTAAAGCTCGGAAGAAGCGTGTTGCCATGAAGA 5 0.125 No Hit GCACTTCGTACCGCGCTTCGCTTCCACAGCCGCGCAGATTCTTCCCGTCG 5 0.125 No Hit GCTGGACAAAATTCTTACTTCGCGAACTACACTATTGCACAATTTAAGCA 5 0.125 No Hit GGGAAAGGGAGATTGGTGAGCTCATTGCAAAGGCTATGGAGAAGGTTGGT 5 0.125 No Hit CCTGACTGGACCGCATTCGATCATTGGCCGTGCTGTTGTTGTCCATGGTG 5 0.125 No Hit CCTGAACTTGTCAGGAAATCATCTATCAGGTTGTATCCCCAAAGATATTG 5 0.125 No Hit TGAGAGTGTCCCTGGCTGCGATGAGCGAGTCATAACAATATTTAGCTCAA 5 0.125 No Hit TGAAGGCAACCTCTCTGAACCTCTTAAATGATGACCCTGAAATGCTAGGG 5 0.125 No Hit ACAAATGGCTCCTGAAAGAAAATCTTCCTTTGACATATCGCCAACAAGTG 5 0.125 No Hit ATAACTCGACGGATCGCACGGCCCTCGTGCCGGCGACGCATCATTCAAAT 5 0.125 No Hit GGATGAGAGTTTCCATCCAAAGCTGTCTGACTTTGGCCTTGCAAAGTTGG 5 0.125 No Hit GTTGTGCGAGAGGACAGTTTTGACAAGCACAAAGTTTTTGAGCTTCCGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.0625 0.0 0.0 0.0 0.0 52-53 0.1 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.125 0.0 0.0 0.0 0.0 58-59 0.15 0.0 0.0 0.0 0.0 60-61 0.16249999999999998 0.0 0.0 0.0 0.0 62-63 0.21250000000000002 0.0 0.0 0.0 0.0 64-65 0.225 0.0 0.0 0.0 0.0 66-67 0.25 0.0 0.0 0.0 0.0 68-69 0.275 0.0 0.0 0.0 0.0 70-71 0.375 0.0 0.0 0.0 0.0 72-73 0.375 0.0 0.0 0.0 0.0 74-75 0.475 0.0 0.0 0.0 0.0 76-77 0.6875 0.0 0.0 0.0 0.0 78-79 0.8125 0.0 0.0 0.0 0.0 80-81 0.9875 0.0 0.0 0.0 0.0 82-83 1.4249999999999998 0.0 0.0 0.0 0.0 84-85 1.7875 0.0 0.0 0.0 0.0 86-87 2.1875 0.0 0.0 0.0 0.0 88-89 2.4625 0.0 0.0 0.0 0.0 90-91 2.925 0.0 0.0 0.0 0.0 92-93 3.3 0.0 0.0 0.0 0.0 94-95 3.5125 0.0 0.0 0.0 0.0 96-97 3.8375 0.0 0.0 0.0 0.0 98-99 4.25 0.0 0.0 0.0 0.0 100-101 4.987500000000001 0.0 0.0 0.0 0.0 102-103 5.35 0.0 0.0 0.0 0.0 104-105 5.85 0.0 0.0 0.0 0.0 106-107 6.525 0.0 0.0 0.0 0.0 108-109 7.050000000000001 0.0 0.0 0.0 0.0 110-111 7.875 0.0 0.0 0.0 0.0 112-113 8.524999999999999 0.0 0.0 0.0 0.0 114-115 9.15 0.0 0.0 0.0 0.0 116-117 9.8125 0.0 0.0 0.0 0.0 118-119 10.412500000000001 0.0 0.0 0.0 0.0 120-121 11.025 0.0 0.0 0.0 0.0 122-123 11.5875 0.0 0.0 0.0 0.0 124-125 12.212499999999999 0.0 0.0 0.0 0.0 126-127 12.8125 0.0 0.0 0.0 0.0 128-129 13.6125 0.0 0.0 0.0 0.0 130-131 14.600000000000001 0.0 0.0 0.0 0.0 132-133 15.6875 0.0 0.0 0.0 0.0 134-135 16.8375 0.0 0.0 0.0 0.0 136-137 17.725 0.0 0.0 0.0 0.0 138-139 18.3375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCTCT 10 0.006830828 145.0 6 CTCTTAC 10 0.006830828 145.0 9 ATGGTCT 10 0.006830828 145.0 4 TCTCTTA 10 0.006830828 145.0 8 >>END_MODULE Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra Read 1504301 spots for SRR13165383.sra Written 1504301 spots for SRR13165383.sra SRR ids: ['SRR13165383.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6b_sebou SRR13165383.sra spots: 30086020 blocks: [[1, 1504301], [1504302, 3008602], [3008603, 4512903], [4512904, 6017204], [6017205, 7521505], [7521506, 9025806], [9025807, 10530107], [10530108, 12034408], [12034409, 13538709], [13538710, 15043010], [15043011, 16547311], [16547312, 18051612], [18051613, 19555913], [19555914, 21060214], [21060215, 22564515], [22564516, 24068816], [24068817, 25573117], [25573118, 27077418], [27077419, 28581719], [28581720, 30086020]] SRR13165383 file size 10202845 SRR13165383 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13165383 SRR13165383_1.fastq SRR13165383_2.fastq Input file: SRR13165383_1.fastq Paired file: SRR13165383_2.fastq trimmed: SRR13165383-trimmed-pair1.fastq, SRR13165383-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Sat Dec 7 17:11:45 2024 >> started Sat Dec 7 17:12:33 2024 >> done (47.309s) 30086020 read pairs processed; of these: 610 ( 0.00%) short read pairs filtered out after trimming by size control 357550 ( 1.19%) empty read pairs filtered out after trimming by size control 29727860 (98.81%) read pairs available; of these: 6571813 (22.11%) trimmed read pairs available after processing 23156047 (77.89%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 44 0.00% 19 41 0.00% 20 85 0.00% 21 90 0.00% 22 115 0.00% 23 139 0.00% 24 140 0.00% 25 192 0.00% 26 226 0.00% 27 239 0.00% 28 285 0.00% 29 258 0.00% 30 322 0.00% 31 323 0.00% 32 320 0.00% 33 367 0.00% 34 321 0.00% 35 349 0.00% 36 395 0.00% 37 427 0.00% 38 469 0.00% 39 481 0.00% 40 546 0.00% 41 559 0.00% 42 577 0.00% 43 696 0.00% 44 631 0.00% 45 743 0.00% 46 749 0.00% 47 825 0.00% 48 964 0.00% 49 1121 0.00% 50 1256 0.00% 51 1452 0.00% 52 1537 0.01% 53 1652 0.01% 54 1631 0.01% 55 1880 0.01% 56 1991 0.01% 57 2400 0.01% 58 2638 0.01% 59 2981 0.01% 60 3589 0.01% 61 4065 0.01% 62 4568 0.02% 63 4898 0.02% 64 5442 0.02% 65 5859 0.02% 66 6443 0.02% 67 6946 0.02% 68 7835 0.03% 69 9026 0.03% 70 10262 0.03% 71 11737 0.04% 72 13289 0.04% 73 14937 0.05% 74 16272 0.05% 75 17654 0.06% 76 18837 0.06% 77 19850 0.07% 78 21572 0.07% 79 23596 0.08% 80 25892 0.09% 81 28701 0.10% 82 31439 0.11% 83 34646 0.12% 84 37598 0.13% 85 39317 0.13% 86 40758 0.14% 87 42598 0.14% 88 45218 0.15% 89 46027 0.15% 90 48560 0.16% 91 51992 0.17% 92 55025 0.19% 93 58331 0.20% 94 61238 0.21% 95 63502 0.21% 96 64551 0.22% 97 66546 0.22% 98 67887 0.23% 99 69749 0.23% 100 70071 0.24% 101 72245 0.24% 102 74639 0.25% 103 79123 0.27% 104 79968 0.27% 105 83351 0.28% 106 84507 0.28% 107 84651 0.28% 108 86093 0.29% 109 85906 0.29% 110 87809 0.30% 111 90058 0.30% 112 91288 0.31% 113 93746 0.32% 114 95508 0.32% 115 98379 0.33% 116 99433 0.33% 117 100473 0.34% 118 99824 0.34% 119 100354 0.34% 120 101558 0.34% 121 102514 0.34% 122 103702 0.35% 123 106211 0.36% 124 108619 0.37% 125 109715 0.37% 126 110807 0.37% 127 110702 0.37% 128 110970 0.37% 129 111515 0.38% 130 111481 0.38% 131 111278 0.37% 132 112717 0.38% 133 114729 0.39% 134 117639 0.40% 135 116895 0.39% 136 118353 0.40% 137 119188 0.40% 138 119921 0.40% 139 118638 0.40% 140 118634 0.40% 141 120204 0.40% 142 120673 0.41% 143 119509 0.40% 144 121793 0.41% 145 123000 0.41% 146 122362 0.41% 147 121961 0.41% 148 123481 0.42% 149 124186 0.42% 150 122723 0.41% 151 23156047 77.89% 29727860 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=20.72 fanout-score-rank=8 prefix-density=0.26 prefix-fanout=20.7 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACCTATATCTCGTATGCCGTCTTCTGCTTGAAAA criterion=fanout-score sequence-density=0.02 sequence-density-rank=38 fanout-score=405.95 fanout-score-rank=1 prefix-density=0.40 prefix-fanout=20.1 sequence=CCGCCGCCGCGTAGCTTCTGGTGGACGGGGCCAGCAGCTGGGCCAGCGCGCGGGCAGCAGCCGAGGAACCGGAGAGAGCGAGAGCCATCGATTGATCTGTGTGTTTTGATCGGATGGCTGGTGGCGCTCCGGCTCTCTGCTGCTGCTCCAACGTGGGTTGCTG criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=2.83 fanout-score-rank=36 prefix-density=0.33 prefix-fanout=2.5 sequence=CGGTTCCGGTTC criterion=fanout-score sequence-density=0.04 sequence-density-rank=34 fanout-score=502.64 fanout-score-rank=1 prefix-density=1.11 prefix-fanout=17.2 sequence=CGCCGCCGCCCCTCGTCCTCTGTGTTCCTTCTCCGAGTTTCAGCCATGGGTAAGGAGAAGACTCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGCAAGTCGACCACCACTGGCCACCTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTACGCGTGGGTGCTTGACAAGCTGAAGGCTGAGCGTGAGAGAGGTATCACCATCGATATTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGATGCCCCTGGACACCGTGACTTCATCAAGAACATGATTACCGGTACCTCCCAGGCTGACTGTGCCGTGCTTATCATTGACTCCACGACTGGAGGTTTTGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCATGCCCTCCTTGCTTTCACTCTTGGAGTGAAGCAGATGATCTGCTGCTGCAACAAGATGG SRR13165383 testing PE reads STAR mapping to Ensembl genome Started job on | Dec 07 17:13:22 Started mapping on | Dec 07 17:13:22 Finished on | Dec 07 17:16:16 Mapping speed, Million of reads per hour | 615.06 Number of input reads | 29727860 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 27463510 Uniquely mapped reads % | 92.38% Average mapped length | 286.54 Number of splices: Total | 26400013 Number of splices: Annotated (sjdb) | 24671115 Number of splices: GT/AG | 26016409 Number of splices: GC/AG | 320369 Number of splices: AT/AC | 20300 Number of splices: Non-canonical | 42935 Mismatch rate per base, % | 0.24% Deletion rate per base | 0.01% Deletion average length | 2.15 Insertion rate per base | 0.01% Insertion average length | 2.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 419558 % of reads mapped to multiple loci | 1.41% Number of reads mapped to too many loci | 89089 % of reads mapped to too many loci | 0.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.53% % of reads unmapped: other | 1.37% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1845123 1845123 1845123 N_multimapping 419558 419558 419558 N_noFeature 838962 26767108 1068978 N_ambiguous 548669 3417 83162 UnstrandedReadsAssigned:26075879 PositiveStrandReadsAssigned:692985 NegativeStrandReadsAssigned:26311370 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=145 echo kmer=141 SRR13165383 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,972 [index] number of k-mers: 66,720,672 [index] number of equivalence classes: 111,837 [quant] running in paired-end mode [quant] will process pair 1: SRR13165383-trimmed-pair1.fastq SRR13165383-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 29,727,860 reads, 27,039,363 reads pseudoaligned [quant] estimated average fragment length: 229.292 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,167 rounds 52973 SRR13165383.ke.tsv 35125 SRR13165383.se.tsv 88098 total ==> SRR13165383.ke.tsv <== target_id length eff_length est_counts tpm PNS24245 936 708.05 0 0 PNS24247 1044 815.708 130.892 8.5743 PNS24249 1928 1699.71 387.027 12.1671 PNS24246 1044 815.708 130.892 8.5743 PNS24248 1044 815.708 130.892 8.5743 PNS24244 1471 1242.71 224.296 9.64434 PNS24243 293 115.268 1 0.463563 KQK14069 1603 1374.71 16414.1 638.006 KQK14071 474 261.996 301.897 61.572 ==> SRR13165383.se.tsv <== BRADI_1g14170v3 17304 BRADI_1g53295v3 401 BRADI_1g59795v3 796 BRADI_1g07683v3 0 BRADI_1g00485v3 28 BRADI_1g20270v3 2471 BRADI_1g74790v3 386 BRADI_1g09890v3 0 BRADI_1g77505v3 442 BRADI_1g48960v3 0 SRR13165383 completed mapping pipeline successfully